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Sequences

Sequence HMGA1.vcAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCCATCCCAGCCATCACTCTTCCACCTGCTCCTT AGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGC GCAAGCAGCCTCCGGTGAGTCCCGGGACAGCGCTGGTAGGGAGTCAGAAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGG AAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCACCACAACTCCAGGAAGGAAACCAAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAG GGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCT CCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACA GCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATG CCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTT GGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCAT CCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACC ACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCT ACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGC CCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCA CCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACC TCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTT CTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGG CCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGT AGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG

HMGA1.u.f.5 (forward primer)
HMGA1.u.r.9 (reverse primer)
Exon skipping and alternative donor or acceptor

Frame 0

WRGRARPPCPWVALFKLP*AGAAIPAITLPPAP *RREDE*VELEVQPALGLQAGKGRH*EAGPGQAA QAASGESRDSAGRESEGAQRSANT*ETSGPTKG KQKQGCCQDPENHHNSRKETKGQTQKTGEGGRG GHLAGVLGGGAVTHACRLLLTGGAASFWDWTALL RSHRPHPFPRPTITTASGRHPHLPPVPSPPHYT AHQPLQGSHGLSGEQFSPGLSSQLPPPTHAYTH ALLDKANIPLSRTLHLLRPHSLGGGDIALWAFGL GAPSLLLHCSLWLPIVGPGRVPLALKGAQAPSH PGTPYSTALAAAGVANGGGCWPPGFPQPNCLCH HVGLTFHPSPTSLVPVLGWTAPFGYRKAGGVSPL LPLHCGHSPLALRLGSEYILW*WRCSHLLSR*G PRALWPPPEVGWGCSPNPTLLPPLSHFPLLRWG TNNKELTLPAPNPPPAPPCPPRFWFHFSSVHKLP LDSCVVFCSMFHSSTSVIAAATSAKCSSSLPPV LPTIPSPKILFVGKRGWGMAGWVTDYPSPREGG ALPLGCCSRVSKGAQIDHKGCRGHLLPLFCWGGV AMICPSLGLPLWFPICSYLNKKNILFWTG

Frame 1

GAGGPVPPAPGSLFLSSPEPVLPSQPSLFHLLL REGKMSESSSKSSQPLASKQEKDGTEKRGRGRP RKQPPVSPGTALVGSQKEPSEVPTPKRPRGRPKG SKNKGAAKTRKTTTTPGRKPRGRPKKLEKEEEE GISQESSEEEQ*PMRAACSSLEEQLPSGTGQLC SAPTAPTPSPGPPSPPPLAATPIFHLCPHHHTTQ HTSRCRAPMG*VGSSFPLASVPSSPRPPTHTHM PSWTRLTSHLAAPCTCCVPTPLVVGTLLSGLLV WGRPLCSFTVPSGFP*WGLGGFPWP*KGPKPHLI LARPTPLPWQQQVWPMEGGAGPQDSPSQTVFVT TWGSLFILPQLP*SPY*VGQPPSVTGRQEG*VP YSLFTVATAPLPSAWDLSTYCGDGDAVTYCPGEA QEPCGRHLRWAGAAPLTLLCFRHSAISPSSDGA PITRSSPCPLPTPLLLLPAPQGSGSIFPLFTNY LWTVVLFFVQCSILRHPSLLLLPAPNVHPHCLLF CPRSPPPRYSLWGRGAGAWQAG*PTTPVPGKVG PCP*DAAAE*ARGPKSTIKGVGATSSPCSVGEG *P*FVPAWGSLSGFLFAVT*IKKISFSGLE

Frame 2

ARAGPSPLPLGRSF*APLSRCCHPSHHSSTCSL EKGR*VSRARSPASPWPPSRKRTALRSGAGAGR ASSLR*VPGQRW*GVRRSPAKCQHLRDLGADQR EAKTRVLPRPGKPPQLQEGNQGADPKNWRRRKRR ASRRSPRRRSSDPCVPPAPHWRSSFLLGLDSFA PLPPPPPLPQAHHHHRLWPPPPSSTCALTTTLH STPAAAGLPWAEWGAVFPWPQFPAPPAHPRIHTC PPGQG*HPT*PHPAPAASPLPWWWGHCSLGFWF GGALSAPSLFPLASHSGAWEGSPGLKRGPSPIS SWHALLHCPGSSRCGQWRGVLAPRIPPAKLSLSP RGAHFSSFPNFPSPRTRLDSPLRLQEGRRGESP TPSSLWPQPPCPPPGI*VHIVVMEMQSLIVQVR PKSPVAAT*GGLGLLP*PYFASATQPFPPPQMGH Q*QGAHPARSQPPSCSSLPPKVLVPFFLCSQTT SGQLCCFLFNVPFFDIRHCCCYQRQMFILIASC SAHDPLPQDTLCGEEGLGHGRLGDRLPQSQGRWG PAPRMLQQSEQGGPNRP*RV*GPPPPPVLLGRG SHDLSQPGAPSLVSYLQLLE*KKYPFLDW

Sequence HMGA1.qAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCC CGGCAACCCGGAGCGCGCACCGCAGGCCGGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCG AGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGAAGGAGCCCA GCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGA GGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCT TCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTC CCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATC CCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTC ACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGC CCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCT TCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACA GCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACC TGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTG CCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTT GTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAA GATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGC AGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGG GGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG

HMGA1.u.f.5 (forward primer)
HMGA1.u.r.9 (reverse primer)
Exon skipping and alternative donor or acceptor

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA ISGAGAAPRRHPHLLPAAAAAEPGRSSAPSTAAP GNPERAPQAGGRARASQPSLFHLLLREGKMSES SSKSSQPLASKQEKDGTEKRGRGRPRKQPPKEP SEVPTPKRPRGRPKGSKNKGEKEEEEGISQESSE EEQ*PMRAACSSLEEQLPSGTGQLCSAPTAPTP SPGPPSPPPLAATPIFHLCPHHHTTQHTSRCRA PMG*VGSSFPLASVPSSPRPPTHTHMPSWTRLTS HLAAPCTCCVPTPLVVGTLLSGLLVWGRPLCSF TVPSGFP*WGLGGFPWP*KGPKPHLILARPTPL PWQQQVWPMEGGAGPQDSPSQTVFVTTWGSLFIL PQLP*SPY*VGQPPSVTGRQEG*VPYSLFTVAT APLPSAWDLSTYCGDGDAVTYCPGEAQEPCGRH LRWAGAAPLTLLCFRHSAISPSSDGAPITRSSPC PLPTPLLLLPAPQGSGSIFPLFTNYLWTVVLFF VQCSILRHPSLLLLPAPNVHPHCLLFCPRSPPP RYSLWGRGAGAWQAG*PTTPVPGKVGPCP*DAAA E*ARGPKSTIKGVGATSSPCSVGEG*P*FVPAW GSLSGFLFAVT*IKKISFSGLE

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL FLALARLQEGIRICYQRRPRRSQAGPQRPAPPL PATRSAHRRPAAELAHPSHHSSTCSLEKGR*VSR ARSPASPWPPSRKRTALRSGAGAGRASSLRRSP AKCQHLRDLGADQREAKTRVRRRKRRASRRSPR RRSSDPCVPPAPHWRSSFLLGLDSFAPLPPPPPL PQAHHHHRLWPPPPSSTCALTTTLHSTPAAAGL PWAEWGAVFPWPQFPAPPAHPRIHTCPPGQG*H PT*PHPAPAASPLPWWWGHCSLGFWFGGALSAPS LFPLASHSGAWEGSPGLKRGPSPISSWHALLHC PGSSRCGQWRGVLAPRIPPAKLSLSPRGAHFSS FPNFPSPRTRLDSPLRLQEGRRGESPTPSSLWPQ PPCPPPGI*VHIVVMEMQSLIVQVRPKSPVAAT *GGLGLLP*PYFASATQPFPPPQMGHQ*QGAHP ARSQPPSCSSLPPKVLVPFFLCSQTTSGQLCCFL FNVPFFDIRHCCCYQRQMFILIASCSAHDPLPQ DTLCGEEGLGHGRLGDRLPQSQGRWGPAPRMLQ QSEQGGPNRP*RV*GPPPPPVLLGRGSHDLSQPG APSLVSYLQLLE*KKYPFLDW

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY FWRWRGSKKASAFATSGGRGGARPVLSAQHRRS RQPGARTAGRRPSSRIPAITLPPAP*RREDE*V ELEVQPALGLQAGKGRH*EAGPGQAAQAASEGAQ RSANT*ETSGPTKGKQKQG*EGGRGGHLAGVLG GGAVTHACRLLLTGGAASFWDWTALLRSHRPHP FPRPTITTASGRHPHLPPVPSPPHYTAHQPLQGS HGLSGEQFSPGLSSQLPPPTHAYTHALLDKANI PLSRTLHLLRPHSLGGGDIALWAFGLGAPSLLL HCSLWLPIVGPGRVPLALKGAQAPSHPGTPYSTA LAAAGVANGGGCWPPGFPQPNCLCHHVGLTFHP SPTSLVPVLGWTAPFGYRKAGGVSPLLPLHCGH SPLALRLGSEYILW*WRCSHLLSR*GPRALWPPP EVGWGCSPNPTLLPPLSHFPLLRWGTNNKELTL PAPNPPPAPPCPPRFWFHFSSVHKLPLDSCVVF CSMFHSSTSVIAAATSAKCSSSLPPVLPTIPSPK ILFVGKRGWGMAGWVTDYPSPREGGALPLGCCS RVSKGAQIDHKGCRGHLLPLFCWGGVAMICPSL GLPLWFPICSYLNKKNILFWTG

Sequence HMGA1.oAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCC CGGCAACCCGGAGCGCGCACCGCAGGCCGGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCG AGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCG GGACAGCGCTGGTAGGGAGTCAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGAGAAGGAGGA AGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAG CTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACA CTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATAC ACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTT TTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCC ATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTT TGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGA GTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAG GTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGA TGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACA AACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCC TCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAA GGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGG GAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG

HMGA1.u.f.5 (forward primer)
HMGA1.u.r.9 (reverse primer)
Exon skipping and alternative donor or acceptor

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA ISGAGAAPRRHPHLLPAAAAAEPGRSSAPSTAAP GNPERAPQAGGRARASQPSLFHLLLREGKMSES SSKSSQPLASKQEKDGTEKRGRGRPRKQPPVSP GTALVGSQEPSEVPTPKRPRGRPKGSKNKGEKEE EEGISQESSEEEQ*PMRAACSSLEEQLPSGTGQ LCSAPTAPTPSPGPPSPPPLAATPIFHLCPHHH TTQHTSRCRAPMG*VGSSFPLASVPSSPRPPTHT HMPSWTRLTSHLAAPCTCCVPTPLVVGTLLSGL LVWGRPLCSFTVPSGFP*WGLGGFPWP*KGPKP HLILARPTPLPWQQQVWPMEGGAGPQDSPSQTVF VTTWGSLFILPQLP*SPY*VGQPPSVTGRQEG* VPYSLFTVATAPLPSAWDLSTYCGDGDAVTYCP GEAQEPCGRHLRWAGAAPLTLLCFRHSAISPSSD GAPITRSSPCPLPTPLLLLPAPQGSGSIFPLFT NYLWTVVLFFVQCSILRHPSLLLLPAPNVHPHC LLFCPRSPPPRYSLWGRGAGAWQAG*PTTPVPGK VGPCP*DAAAE*ARGPKSTIKGVGATSSPCSVG EG*P*FVPAWGSLSGFLFAVT*IKKISFSGLE

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL FLALARLQEGIRICYQRRPRRSQAGPQRPAPPL PATRSAHRRPAAELAHPSHHSSTCSLEKGR*VSR ARSPASPWPPSRKRTALRSGAGAGRASSLR*VP GQRW*GVRSPAKCQHLRDLGADQREAKTRVRRR KRRASRRSPRRRSSDPCVPPAPHWRSSFLLGLDS FAPLPPPPPLPQAHHHHRLWPPPPSSTCALTTT LHSTPAAAGLPWAEWGAVFPWPQFPAPPAHPRI HTCPPGQG*HPT*PHPAPAASPLPWWWGHCSLGF WFGGALSAPSLFPLASHSGAWEGSPGLKRGPSP ISSWHALLHCPGSSRCGQWRGVLAPRIPPAKLS LSPRGAHFSSFPNFPSPRTRLDSPLRLQEGRRGE SPTPSSLWPQPPCPPPGI*VHIVVMEMQSLIVQ VRPKSPVAAT*GGLGLLP*PYFASATQPFPPPQ MGHQ*QGAHPARSQPPSCSSLPPKVLVPFFLCSQ TTSGQLCCFLFNVPFFDIRHCCCYQRQMFILIA SCSAHDPLPQDTLCGEEGLGHGRLGDRLPQSQG RWGPAPRMLQQSEQGGPNRP*RV*GPPPPPVLLG RGSHDLSQPGAPSLVSYLQLLE*KKYPFLDW

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY FWRWRGSKKASAFATSGGRGGARPVLSAQHRRS RQPGARTAGRRPSSRIPAITLPPAP*RREDE*V ELEVQPALGLQAGKGRH*EAGPGQAAQAASGESR DSAGRESGAQRSANT*ETSGPTKGKQKQG*EGG RGGHLAGVLGGGAVTHACRLLLTGGAASFWDWT ALLRSHRPHPFPRPTITTASGRHPHLPPVPSPPH YTAHQPLQGSHGLSGEQFSPGLSSQLPPPTHAY THALLDKANIPLSRTLHLLRPHSLGGGDIALWA FGLGAPSLLLHCSLWLPIVGPGRVPLALKGAQAP SHPGTPYSTALAAAGVANGGGCWPPGFPQPNCL CHHVGLTFHPSPTSLVPVLGWTAPFGYRKAGGV SPLLPLHCGHSPLALRLGSEYILW*WRCSHLLSR *GPRALWPPPEVGWGCSPNPTLLPPLSHFPLLR WGTNNKELTLPAPNPPPAPPCPPRFWFHFSSVH KLPLDSCVVFCSMFHSSTSVIAAATSAKCSSSLP PVLPTIPSPKILFVGKRGWGMAGWVTDYPSPRE GGALPLGCCSRVSKGAQIDHKGCRGHLLPLFCW GGVAMICPSLGLPLWFPICSYLNKKNILFWTG

Sequence HMGA1.mAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCC CGGCAACCCGGAGCGCGCACCGCAGGCCGGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCG AGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCG GGACAGCGCTGGTAGGGAGTCAGAAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGAGAAGGA GGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGA CAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACC ACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCA TACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGG CTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGC CCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGT CTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGG TGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTC CAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTC AGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTC ACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATT GCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGG GAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTT GGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG

HMGA1.u.f.5 (forward primer)
HMGA1.u.r.9 (reverse primer)
Exon skipping and alternative donor or acceptor

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA ISGAGAAPRRHPHLLPAAAAAEPGRSSAPSTAAP GNPERAPQAGGRARASQPSLFHLLLREGKMSES SSKSSQPLASKQEKDGTEKRGRGRPRKQPPVSP GTALVGSQKEPSEVPTPKRPRGRPKGSKNKGEKE EEEGISQESSEEEQ*PMRAACSSLEEQLPSGTG QLCSAPTAPTPSPGPPSPPPLAATPIFHLCPHH HTTQHTSRCRAPMG*VGSSFPLASVPSSPRPPTH THMPSWTRLTSHLAAPCTCCVPTPLVVGTLLSG LLVWGRPLCSFTVPSGFP*WGLGGFPWP*KGPK PHLILARPTPLPWQQQVWPMEGGAGPQDSPSQTV FVTTWGSLFILPQLP*SPY*VGQPPSVTGRQEG *VPYSLFTVATAPLPSAWDLSTYCGDGDAVTYC PGEAQEPCGRHLRWAGAAPLTLLCFRHSAISPSS DGAPITRSSPCPLPTPLLLLPAPQGSGSIFPLF TNYLWTVVLFFVQCSILRHPSLLLLPAPNVHPH CLLFCPRSPPPRYSLWGRGAGAWQAG*PTTPVPG KVGPCP*DAAAE*ARGPKSTIKGVGATSSPCSV GEG*P*FVPAWGSLSGFLFAVT*IKKISFSGLE

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL FLALARLQEGIRICYQRRPRRSQAGPQRPAPPL PATRSAHRRPAAELAHPSHHSSTCSLEKGR*VSR ARSPASPWPPSRKRTALRSGAGAGRASSLR*VP GQRW*GVRRSPAKCQHLRDLGADQREAKTRVRR RKRRASRRSPRRRSSDPCVPPAPHWRSSFLLGLD SFAPLPPPPPLPQAHHHHRLWPPPPSSTCALTT TLHSTPAAAGLPWAEWGAVFPWPQFPAPPAHPR IHTCPPGQG*HPT*PHPAPAASPLPWWWGHCSLG FWFGGALSAPSLFPLASHSGAWEGSPGLKRGPS PISSWHALLHCPGSSRCGQWRGVLAPRIPPAKL SLSPRGAHFSSFPNFPSPRTRLDSPLRLQEGRRG ESPTPSSLWPQPPCPPPGI*VHIVVMEMQSLIV QVRPKSPVAAT*GGLGLLP*PYFASATQPFPPP QMGHQ*QGAHPARSQPPSCSSLPPKVLVPFFLCS QTTSGQLCCFLFNVPFFDIRHCCCYQRQMFILI ASCSAHDPLPQDTLCGEEGLGHGRLGDRLPQSQ GRWGPAPRMLQQSEQGGPNRP*RV*GPPPPPVLL GRGSHDLSQPGAPSLVSYLQLLE*KKYPFLDW

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY FWRWRGSKKASAFATSGGRGGARPVLSAQHRRS RQPGARTAGRRPSSRIPAITLPPAP*RREDE*V ELEVQPALGLQAGKGRH*EAGPGQAAQAASGESR DSAGRESEGAQRSANT*ETSGPTKGKQKQG*EG GRGGHLAGVLGGGAVTHACRLLLTGGAASFWDW TALLRSHRPHPFPRPTITTASGRHPHLPPVPSPP HYTAHQPLQGSHGLSGEQFSPGLSSQLPPPTHA YTHALLDKANIPLSRTLHLLRPHSLGGGDIALW AFGLGAPSLLLHCSLWLPIVGPGRVPLALKGAQA PSHPGTPYSTALAAAGVANGGGCWPPGFPQPNC LCHHVGLTFHPSPTSLVPVLGWTAPFGYRKAGG VSPLLPLHCGHSPLALRLGSEYILW*WRCSHLLS R*GPRALWPPPEVGWGCSPNPTLLPPLSHFPLL RWGTNNKELTLPAPNPPPAPPCPPRFWFHFSSV HKLPLDSCVVFCSMFHSSTSVIAAATSAKCSSSL PPVLPTIPSPKILFVGKRGWGMAGWVTDYPSPR EGGALPLGCCSRVSKGAQIDHKGCRGHLLPLFC WGGVAMICPSLGLPLWFPICSYLNKKNILFWTG

Sequence HMGA1.lAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCC CGGCAACCCGGAGCGCGCACCGCAGGCCGGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCG AGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGAGCCCAGCG AAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCACCACAACTCCAGGAAGGAAACC AAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCA CTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCA CCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTC AGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACT CCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAG GGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGC TGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAG CCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATT GTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTG CTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCC CAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGC TGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCA GGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGT GTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAA TAAAAAAAATATCCTTTTCTGGACTGGAG

HMGA1.u.f.5 (forward primer)
HMGA1.u.r.9 (reverse primer)
Exon skipping and alternative donor or acceptor

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA ISGAGAAPRRHPHLLPAAAAAEPGRSSAPSTAAP GNPERAPQAGGRARASQPSLFHLLLREGKMSES SSKSSQPLASKQEKDGTEKRGRGRPRKQPPEPS EVPTPKRPRGRPKGSKNKGAAKTRKTTTTPGRKP RGRPKKLEKEEEEGISQESSEEEQ*PMRAACSS LEEQLPSGTGQLCSAPTAPTPSPGPPSPPPLAA TPIFHLCPHHHTTQHTSRCRAPMG*VGSSFPLAS VPSSPRPPTHTHMPSWTRLTSHLAAPCTCCVPT PLVVGTLLSGLLVWGRPLCSFTVPSGFP*WGLG GFPWP*KGPKPHLILARPTPLPWQQQVWPMEGGA GPQDSPSQTVFVTTWGSLFILPQLP*SPY*VGQ PPSVTGRQEG*VPYSLFTVATAPLPSAWDLSTY CGDGDAVTYCPGEAQEPCGRHLRWAGAAPLTLLC FRHSAISPSSDGAPITRSSPCPLPTPLLLLPAP QGSGSIFPLFTNYLWTVVLFFVQCSILRHPSLL LLPAPNVHPHCLLFCPRSPPPRYSLWGRGAGAWQ AG*PTTPVPGKVGPCP*DAAAE*ARGPKSTIKG VGATSSPCSVGEG*P*FVPAWGSLSGFLFAVT* IKKISFSGLE

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL FLALARLQEGIRICYQRRPRRSQAGPQRPAPPL PATRSAHRRPAAELAHPSHHSSTCSLEKGR*VSR ARSPASPWPPSRKRTALRSGAGAGRASSLRSPA KCQHLRDLGADQREAKTRVLPRPGKPPQLQEGN QGADPKNWRRRKRRASRRSPRRRSSDPCVPPAPH WRSSFLLGLDSFAPLPPPPPLPQAHHHHRLWPP PPSSTCALTTTLHSTPAAAGLPWAEWGAVFPWP QFPAPPAHPRIHTCPPGQG*HPT*PHPAPAASPL PWWWGHCSLGFWFGGALSAPSLFPLASHSGAWE GSPGLKRGPSPISSWHALLHCPGSSRCGQWRGV LAPRIPPAKLSLSPRGAHFSSFPNFPSPRTRLDS PLRLQEGRRGESPTPSSLWPQPPCPPPGI*VHI VVMEMQSLIVQVRPKSPVAAT*GGLGLLP*PYF ASATQPFPPPQMGHQ*QGAHPARSQPPSCSSLPP KVLVPFFLCSQTTSGQLCCFLFNVPFFDIRHCC CYQRQMFILIASCSAHDPLPQDTLCGEEGLGHG RLGDRLPQSQGRWGPAPRMLQQSEQGGPNRP*RV *GPPPPPVLLGRGSHDLSQPGAPSLVSYLQLLE *KKYPFLDW

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY FWRWRGSKKASAFATSGGRGGARPVLSAQHRRS RQPGARTAGRRPSSRIPAITLPPAP*RREDE*V ELEVQPALGLQAGKGRH*EAGPGQAAQAASGAQR SANT*ETSGPTKGKQKQGCCQDPENHHNSRKET KGQTQKTGEGGRGGHLAGVLGGGAVTHACRLLL TGGAASFWDWTALLRSHRPHPFPRPTITTASGRH PHLPPVPSPPHYTAHQPLQGSHGLSGEQFSPGL SSQLPPPTHAYTHALLDKANIPLSRTLHLLRPH SLGGGDIALWAFGLGAPSLLLHCSLWLPIVGPGR VPLALKGAQAPSHPGTPYSTALAAAGVANGGGC WPPGFPQPNCLCHHVGLTFHPSPTSLVPVLGWT APFGYRKAGGVSPLLPLHCGHSPLALRLGSEYIL W*WRCSHLLSR*GPRALWPPPEVGWGCSPNPTL LPPLSHFPLLRWGTNNKELTLPAPNPPPAPPCP PRFWFHFSSVHKLPLDSCVVFCSMFHSSTSVIAA ATSAKCSSSLPPVLPTIPSPKILFVGKRGWGMA GWVTDYPSPREGGALPLGCCSRVSKGAQIDHKG CRGHLLPLFCWGGVAMICPSLGLPLWFPICSYLN KKNILFWTG

Sequence HMGA1.jAug05

HMGA1.u.f.5 (forward primer)
HMGA1.u.r.9 (reverse primer)
Exon skipping and alternative donor or acceptor

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA ISGAGAAPRRHPHLLPAAAAAEPGRSSAPSTAAP GNPERAPQAGGRARASQPSLFHLLLREGKMSES SSKSSQPLASKQEKDGTEKRGRGRPRKQPPKEP SEVPTPKRPRGRPKGSKNKGAAKTRKTTTTPGRK PRGRPKKLEKEEEEGISQESSEEEQ*PMRAACS SLEEQLPSGTGQLCSAPTAPLPSAWDLSTYCGD GDAVTYCPGEAQEPCGRHLRWAGAAPLTLLCFRH SAISPSSDGAPITRSSPCPLPTPLLLLPAPQGS GSIFPLFTNYLWTVVLFFVQCSILRHPSLLLLP APNVHPHCLLFCPRSPPPRYSLWGRGAGAWQAG* PTTPVPGKVGPCP*DAAAE*ARGPKSTIKGVGA TSSPCSVGEG*P*FVPAWGSLSGFLFAVT*IKK ISFSGLE

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL FLALARLQEGIRICYQRRPRRSQAGPQRPAPPL PATRSAHRRPAAELAHPSHHSSTCSLEKGR*VSR ARSPASPWPPSRKRTALRSGAGAGRASSLRRSP AKCQHLRDLGADQREAKTRVLPRPGKPPQLQEG NQGADPKNWRRRKRRASRRSPRRRSSDPCVPPAP HWRSSFLLGLDSFAPLPQPPCPPPGI*VHIVVM EMQSLIVQVRPKSPVAAT*GGLGLLP*PYFASA TQPFPPPQMGHQ*QGAHPARSQPPSCSSLPPKVL VPFFLCSQTTSGQLCCFLFNVPFFDIRHCCCYQ RQMFILIASCSAHDPLPQDTLCGEEGLGHGRLG DRLPQSQGRWGPAPRMLQQSEQGGPNRP*RV*GP PPPPVLLGRGSHDLSQPGAPSLVSYLQLLE*KK YPFLDW

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY FWRWRGSKKASAFATSGGRGGARPVLSAQHRRS RQPGARTAGRRPSSRIPAITLPPAP*RREDE*V ELEVQPALGLQAGKGRH*EAGPGQAAQAASEGAQ RSANT*ETSGPTKGKQKQGCCQDPENHHNSRKE TKGQTQKTGEGGRGGHLAGVLGGGAVTHACRLL LTGGAASFWDWTALLRSHSPLALRLGSEYILW*W RCSHLLSR*GPRALWPPPEVGWGCSPNPTLLPP LSHFPLLRWGTNNKELTLPAPNPPPAPPCPPRF WFHFSSVHKLPLDSCVVFCSMFHSSTSVIAAATS AKCSSSLPPVLPTIPSPKILFVGKRGWGMAGWV TDYPSPREGGALPLGCCSRVSKGAQIDHKGCRG HLLPLFCWGGVAMICPSLGLPLWFPICSYLNKKN ILFWTG

Sequence HMGA1.iAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCC CGGCAACCCGGAGCGCGCACCGCAGGCCGGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCG AGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGAAGGAGCCCA GCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCACCACAACTCCAGGAAGGAA ACCAAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCC TCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCG CCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGC CTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCC ACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGG GAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGG TGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGA CAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACAT ATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACT TTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCC CCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGC TGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATG GCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAG GGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTT GAATAAAAAAAATATCCTTTTCTGGACTGGAG

HMGA1.u.f.5 (forward primer)
HMGA1.u.r.9 (reverse primer)
Exon skipping and alternative donor or acceptor

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA ISGAGAAPRRHPHLLPAAAAAEPGRSSAPSTAAP GNPERAPQAGGRARASQPSLFHLLLREGKMSES SSKSSQPLASKQEKDGTEKRGRGRPRKQPPKEP SEVPTPKRPRGRPKGSKNKGAAKTRKTTTTPGRK PRGRPKKLEKEEEEGISQESSEEEQ*PMRAACS SLEEQLPSGTGQLCSAPTAPTPSPGPPSPPPLA ATPIFHLCPHHHTTQHTSRCRAPMG*VGSSFPLA SVPSSPRPPTHTHMPSWTRLTSHLAAPCTCCVP TPLVVGTLLSGLLVWGRPLCSFTVPSGFP*WGL GGFPWP*KGPKPHLILARPTPLPWQQQVWPMEGG AGPQDSPSQTVFVTTWGSLFILPQLP*SPY*VG QPPSVTGRQEG*VPYSLFTVATAPLPSAWDLST YCGDGDAVTYCPGEAQEPCGRHLRWAGAAPLTLL CFRHSAISPSSDGAPITRSSPCPLPTPLLLLPA PQGSGSIFPLFTNYLWTVVLFFVQCSILRHPSL LLLPAPNVHPHCLLFCPRSPPPRYSLWGRGAGAW QAG*PTTPVPGKVGPCP*DAAAE*ARGPKSTIK GVGATSSPCSVGEG*P*FVPAWGSLSGFLFAVT *IKKISFSGLE

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL FLALARLQEGIRICYQRRPRRSQAGPQRPAPPL PATRSAHRRPAAELAHPSHHSSTCSLEKGR*VSR ARSPASPWPPSRKRTALRSGAGAGRASSLRRSP AKCQHLRDLGADQREAKTRVLPRPGKPPQLQEG NQGADPKNWRRRKRRASRRSPRRRSSDPCVPPAP HWRSSFLLGLDSFAPLPPPPPLPQAHHHHRLWP PPPSSTCALTTTLHSTPAAAGLPWAEWGAVFPW PQFPAPPAHPRIHTCPPGQG*HPT*PHPAPAASP LPWWWGHCSLGFWFGGALSAPSLFPLASHSGAW EGSPGLKRGPSPISSWHALLHCPGSSRCGQWRG VLAPRIPPAKLSLSPRGAHFSSFPNFPSPRTRLD SPLRLQEGRRGESPTPSSLWPQPPCPPPGI*VH IVVMEMQSLIVQVRPKSPVAAT*GGLGLLP*PY FASATQPFPPPQMGHQ*QGAHPARSQPPSCSSLP PKVLVPFFLCSQTTSGQLCCFLFNVPFFDIRHC CCYQRQMFILIASCSAHDPLPQDTLCGEEGLGH GRLGDRLPQSQGRWGPAPRMLQQSEQGGPNRP*R V*GPPPPPVLLGRGSHDLSQPGAPSLVSYLQLL E*KKYPFLDW

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY FWRWRGSKKASAFATSGGRGGARPVLSAQHRRS RQPGARTAGRRPSSRIPAITLPPAP*RREDE*V ELEVQPALGLQAGKGRH*EAGPGQAAQAASEGAQ RSANT*ETSGPTKGKQKQGCCQDPENHHNSRKE TKGQTQKTGEGGRGGHLAGVLGGGAVTHACRLL LTGGAASFWDWTALLRSHRPHPFPRPTITTASGR HPHLPPVPSPPHYTAHQPLQGSHGLSGEQFSPG LSSQLPPPTHAYTHALLDKANIPLSRTLHLLRP HSLGGGDIALWAFGLGAPSLLLHCSLWLPIVGPG RVPLALKGAQAPSHPGTPYSTALAAAGVANGGG CWPPGFPQPNCLCHHVGLTFHPSPTSLVPVLGW TAPFGYRKAGGVSPLLPLHCGHSPLALRLGSEYI LW*WRCSHLLSR*GPRALWPPPEVGWGCSPNPT LLPPLSHFPLLRWGTNNKELTLPAPNPPPAPPC PPRFWFHFSSVHKLPLDSCVVFCSMFHSSTSVIA AATSAKCSSSLPPVLPTIPSPKILFVGKRGWGM AGWVTDYPSPREGGALPLGCCSRVSKGAQIDHK GCRGHLLPLFCWGGVAMICPSLGLPLWFPICSYL NKKNILFWTG

Sequence HMGA1.gAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCC CGGCAACCCGGAGCGCGCACCGCAGGCCGGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCG AGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCG GGACAGCGCTGGTAGGGAGTCAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGAC CCGGAAAACCACCACAACTCCAGGAAGGAAACCAAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAG GAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCC CAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCAT GGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCAC TTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTG TTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTG GCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCC CAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCC CCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAG GTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCG CTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTC AATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATA CTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAG TGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCT CCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG

HMGA1.u.f.5 (forward primer)
HMGA1.u.r.9 (reverse primer)
Exon skipping and alternative donor or acceptor

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA ISGAGAAPRRHPHLLPAAAAAEPGRSSAPSTAAP GNPERAPQAGGRARASQPSLFHLLLREGKMSES SSKSSQPLASKQEKDGTEKRGRGRPRKQPPVSP GTALVGSQEPSEVPTPKRPRGRPKGSKNKGAAKT RKTTTTPGRKPRGRPKKLEKEEEEGISQESSEE EQ*PMRAACSSLEEQLPSGTGQLCSAPTAPTPS PGPPSPPPLAATPIFHLCPHHHTTQHTSRCRAPM G*VGSSFPLASVPSSPRPPTHTHMPSWTRLTSH LAAPCTCCVPTPLVVGTLLSGLLVWGRPLCSFT VPSGFP*WGLGGFPWP*KGPKPHLILARPTPLPW QQQVWPMEGGAGPQDSPSQTVFVTTWGSLFILP QLP*SPY*VGQPPSVTGRQEG*VPYSLFTVATA PLPSAWDLSTYCGDGDAVTYCPGEAQEPCGRHLR WAGAAPLTLLCFRHSAISPSSDGAPITRSSPCP LPTPLLLLPAPQGSGSIFPLFTNYLWTVVLFFV QCSILRHPSLLLLPAPNVHPHCLLFCPRSPPPRY SLWGRGAGAWQAG*PTTPVPGKVGPCP*DAAAE *ARGPKSTIKGVGATSSPCSVGEG*P*FVPAWG SLSGFLFAVT*IKKISFSGLE

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL FLALARLQEGIRICYQRRPRRSQAGPQRPAPPL PATRSAHRRPAAELAHPSHHSSTCSLEKGR*VSR ARSPASPWPPSRKRTALRSGAGAGRASSLR*VP GQRW*GVRSPAKCQHLRDLGADQREAKTRVLPR PGKPPQLQEGNQGADPKNWRRRKRRASRRSPRRR SSDPCVPPAPHWRSSFLLGLDSFAPLPPPPPLP QAHHHHRLWPPPPSSTCALTTTLHSTPAAAGLP WAEWGAVFPWPQFPAPPAHPRIHTCPPGQG*HPT *PHPAPAASPLPWWWGHCSLGFWFGGALSAPSL FPLASHSGAWEGSPGLKRGPSPISSWHALLHCP GSSRCGQWRGVLAPRIPPAKLSLSPRGAHFSSFP NFPSPRTRLDSPLRLQEGRRGESPTPSSLWPQP PCPPPGI*VHIVVMEMQSLIVQVRPKSPVAAT* GGLGLLP*PYFASATQPFPPPQMGHQ*QGAHPAR SQPPSCSSLPPKVLVPFFLCSQTTSGQLCCFLF NVPFFDIRHCCCYQRQMFILIASCSAHDPLPQD TLCGEEGLGHGRLGDRLPQSQGRWGPAPRMLQQS EQGGPNRP*RV*GPPPPPVLLGRGSHDLSQPGA PSLVSYLQLLE*KKYPFLDW

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY FWRWRGSKKASAFATSGGRGGARPVLSAQHRRS RQPGARTAGRRPSSRIPAITLPPAP*RREDE*V ELEVQPALGLQAGKGRH*EAGPGQAAQAASGESR DSAGRESGAQRSANT*ETSGPTKGKQKQGCCQD PENHHNSRKETKGQTQKTGEGGRGGHLAGVLGG GAVTHACRLLLTGGAASFWDWTALLRSHRPHPFP RPTITTASGRHPHLPPVPSPPHYTAHQPLQGSH GLSGEQFSPGLSSQLPPPTHAYTHALLDKANIP LSRTLHLLRPHSLGGGDIALWAFGLGAPSLLLHC SLWLPIVGPGRVPLALKGAQAPSHPGTPYSTAL AAAGVANGGGCWPPGFPQPNCLCHHVGLTFHPS PTSLVPVLGWTAPFGYRKAGGVSPLLPLHCGHSP LALRLGSEYILW*WRCSHLLSR*GPRALWPPPE VGWGCSPNPTLLPPLSHFPLLRWGTNNKELTLP APNPPPAPPCPPRFWFHFSSVHKLPLDSCVVFCS MFHSSTSVIAAATSAKCSSSLPPVLPTIPSPKI LFVGKRGWGMAGWVTDYPSPREGGALPLGCCSR VSKGAQIDHKGCRGHLLPLFCWGGVAMICPSLGL PLWFPICSYLNKKNILFWTG

Sequence HMGA1.fAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCC CGGCAACCCGGAGCGCGCACCGCAGGCCGGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCG AGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCG GGACAGCGCTGGTAGGGAGTCAGAAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAA GACCCGGAAAACCACCACAACTCCAGGAAGGAAACCAAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAG GAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTT CCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCC CATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCC CACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCA CTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCC CTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTT CCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAG CCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCT GAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGC CCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTG TTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAG ATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCA GAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGG GCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG

HMGA1.u.f.5 (forward primer)
HMGA1.u.r.9 (reverse primer)
Exon skipping and alternative donor or acceptor

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA ISGAGAAPRRHPHLLPAAAAAEPGRSSAPSTAAP GNPERAPQAGGRARASQPSLFHLLLREGKMSES SSKSSQPLASKQEKDGTEKRGRGRPRKQPPVSP GTALVGSQKEPSEVPTPKRPRGRPKGSKNKGAAK TRKTTTTPGRKPRGRPKKLEKEEEEGISQESSE EEQ*PMRAACSSLEEQLPSGTGQLCSAPTAPTP SPGPPSPPPLAATPIFHLCPHHHTTQHTSRCRAP MG*VGSSFPLASVPSSPRPPTHTHMPSWTRLTS HLAAPCTCCVPTPLVVGTLLSGLLVWGRPLCSF TVPSGFP*WGLGGFPWP*KGPKPHLILARPTPLP WQQQVWPMEGGAGPQDSPSQTVFVTTWGSLFIL PQLP*SPY*VGQPPSVTGRQEG*VPYSLFTVAT APLPSAWDLSTYCGDGDAVTYCPGEAQEPCGRHL RWAGAAPLTLLCFRHSAISPSSDGAPITRSSPC PLPTPLLLLPAPQGSGSIFPLFTNYLWTVVLFF VQCSILRHPSLLLLPAPNVHPHCLLFCPRSPPPR YSLWGRGAGAWQAG*PTTPVPGKVGPCP*DAAA E*ARGPKSTIKGVGATSSPCSVGEG*P*FVPAW GSLSGFLFAVT*IKKISFSGLE

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL FLALARLQEGIRICYQRRPRRSQAGPQRPAPPL PATRSAHRRPAAELAHPSHHSSTCSLEKGR*VSR ARSPASPWPPSRKRTALRSGAGAGRASSLR*VP GQRW*GVRRSPAKCQHLRDLGADQREAKTRVLP RPGKPPQLQEGNQGADPKNWRRRKRRASRRSPRR RSSDPCVPPAPHWRSSFLLGLDSFAPLPPPPPL PQAHHHHRLWPPPPSSTCALTTTLHSTPAAAGL PWAEWGAVFPWPQFPAPPAHPRIHTCPPGQG*HP T*PHPAPAASPLPWWWGHCSLGFWFGGALSAPS LFPLASHSGAWEGSPGLKRGPSPISSWHALLHC PGSSRCGQWRGVLAPRIPPAKLSLSPRGAHFSSF PNFPSPRTRLDSPLRLQEGRRGESPTPSSLWPQ PPCPPPGI*VHIVVMEMQSLIVQVRPKSPVAAT *GGLGLLP*PYFASATQPFPPPQMGHQ*QGAHPA RSQPPSCSSLPPKVLVPFFLCSQTTSGQLCCFL FNVPFFDIRHCCCYQRQMFILIASCSAHDPLPQ DTLCGEEGLGHGRLGDRLPQSQGRWGPAPRMLQQ SEQGGPNRP*RV*GPPPPPVLLGRGSHDLSQPG APSLVSYLQLLE*KKYPFLDW

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY FWRWRGSKKASAFATSGGRGGARPVLSAQHRRS RQPGARTAGRRPSSRIPAITLPPAP*RREDE*V ELEVQPALGLQAGKGRH*EAGPGQAAQAASGESR DSAGRESEGAQRSANT*ETSGPTKGKQKQGCCQ DPENHHNSRKETKGQTQKTGEGGRGGHLAGVLG GGAVTHACRLLLTGGAASFWDWTALLRSHRPHPF PRPTITTASGRHPHLPPVPSPPHYTAHQPLQGS HGLSGEQFSPGLSSQLPPPTHAYTHALLDKANI PLSRTLHLLRPHSLGGGDIALWAFGLGAPSLLLH CSLWLPIVGPGRVPLALKGAQAPSHPGTPYSTA LAAAGVANGGGCWPPGFPQPNCLCHHVGLTFHP SPTSLVPVLGWTAPFGYRKAGGVSPLLPLHCGHS PLALRLGSEYILW*WRCSHLLSR*GPRALWPPP EVGWGCSPNPTLLPPLSHFPLLRWGTNNKELTL PAPNPPPAPPCPPRFWFHFSSVHKLPLDSCVVFC SMFHSSTSVIAAATSAKCSSSLPPVLPTIPSPK ILFVGKRGWGMAGWVTDYPSPREGGALPLGCCS RVSKGAQIDHKGCRGHLLPLFCWGGVAMICPSLG LPLWFPICSYLNKKNILFWTG

Sequence HMGA1.bAug05

HMGA1.u.f.5 (forward primer)
HMGA1.u.r.9 (reverse primer)
Exon skipping and alternative donor or acceptor

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA ISGAGAAPRRHPHLLPAAAAAEPGRSSAPSTAAP GNPERAPQAGGRARASQPSLFHLLLREGKMSES SSKSSQPLASKQEKDGTEKRGRGRPRKQPPVSP GTALVGSQEPSEVPTPKRPRGRPKGSKNKGAAKT RKTTTTPGRKPRGRPKKLALLDLLGHQILQKSR EGKVPGLGCAHEK*GVPGINQTTSPCPALP*LC VGVPPSPAPRILRTSRGDLGSHLACVPLN*R*GK EAIGGRFVLPMSLCRRETAKELGPGRGRCWSSD VTHHTALEGTIQFWVPKQLVERLGGLSQEAASR GPLQLGS

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL FLALARLQEGIRICYQRRPRRSQAGPQRPAPPL PATRSAHRRPAAELAHPSHHSSTCSLEKGR*VSR ARSPASPWPPSRKRTALRSGAGAGRASSLR*VP GQRW*GVRSPAKCQHLRDLGADQREAKTRVLPR PGKPPQLQEGNQGADPKNWLSLTCWDIRFYRSPE RGRYLAWAVPMRSEGSQV*IRPHPPALPCPSCV WGSLPLLLLEYSELLGEILEVI*PVSPSIRDEE RKP*GEGLSFL*ASAEEKQRRSWALGGVGAGVLM *PTTLHWRAPSNSGSPNSWWKGSVG*VKKLPLG GHCS*GH

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY FWRWRGSKKASAFATSGGRGGARPVLSAQHRRS RQPGARTAGRRPSSRIPAITLPPAP*RREDE*V ELEVQPALGLQAGKGRH*EAGPGQAAQAASGESR DSAGRESGAQRSANT*ETSGPTKGKQKQGCCQD PENHHNSRKETKGQTQKTGSP*PAGTSDFTEVQ RGEGTWPGLCP*EVRGPRYKSDHIPLPCPALVVC GGPSLSCS*NTQNF*GRSWKSSSLCPPQLEMRK GSHRGKVCPSYEPLQKRNSEGAGPWEGSVLEF* CDPPHCTGGHHPILGPQTAGGKARWAESRSCL*G ATAVRVT