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Sequences

Sequence PTK2.mAug05

GTTTCTGGCTACCCTGGTTCACATGGAATCACAGCCATGGCTGGCAGCATCTATCCAGGTCAGGCATCTCTTTTGGACCAAACAGATTCATGGAATCATA GACCTCAGGAGATAGCAATGTGGCAGCCCAATGTGGAGGACTCTACAGTATTGGACCTGCGAGGGATTGGGCAAGTGTTGCCAACCCATCTGATGGAAGA GCGTCTAATCCGACAGCAACAGGAAATGGAAGAAGATCAGCGCTGGCTGGAAAAAGAGGAAAGATTTCTGATTGGAAACCAACATATATATCAGCCTGTG GGTAAACCAGATCCTGCAGCTCCACCAAAGAAACCGCCTCGCCCTGGAGCTCCCGGTCATCTGGGAAGCCTTGCCAGCCTCAGCAGCCCTGCTGACAGCT ACAACGAGGGTGTCAAGCTTCAGCCCCAGGAAATCAGCCCCCCTCCTACTGCCAACCTGGACCGGTCGAATGATAAGGTGTACGAGAATGTGACGGGCCT GGTGAAAGCTGTCATCGAGATGTCCAGTAAAATCCAGCCAGCCCCACCAGAGGAGTATGTCCCTATGGTGAAGGAAGTCGGCTTGGCCCTGAGGACATTA TTGGCCACTGTGGATGAGACCATTCCCCTCCTACCAGCCAGCACCCACCGAGAGATTGAGATGGCACAGAAGCTATTGAACTCTGACCTGGGTGAGCTCA TCAACAAGATGAAACTGGCCCAGCAGTATGTCATGACCAGCCTCCAGCAAGAGTACAAAAAGCAAATGCTGACTGCTGCTCACGCCCTGGCTGTGGATGC CAAAAACTTACTCGATGTCATTGACCAAGCAAGACTGAAAATGCTTGGGCAGACGAGACCACACTGAGCCTCCCCTAGGAGCACGTCTTGCTACCCTCTT TTGAAGATGTTCTCTAGCCTTCCACCAGCAGCGAGGAATTAACCCTGTGTCCTCAGTCGCCAGCACTTACAGCTCCAACTTTTTTGAATGACCATCTGGT TGAAAAATCTTTCTCATATAAGTTTAACCACACTTTGATTTGGGTTCATTTTTTGTTTTGTTTTTTTCAATCATGATATTCAGAAAAATCCAGGATCCAA AATGTGGCGTTTTTCTAAGAATGAAAATTATATGTAAGCTTTTAAGCATCATGAAGAACAATTTATGTTCACATTAAGATACGTTCTAAAGGGGGATGGC CAAGGGGTGACATCTTAATTCCTAAACTACCTTAGCTGCATAGTGGAAGAGGAGAGCATGAAGCAAAGAATTCCAGGAAACCCAAGAGGCTGAGAATTCT TTTGTCTACCATAGAATTATTATCCAGACTGGAATTTTTGTTTGTTAGAACACCCTTCAGTTGCAATATGCTAATCCCACTTTACAAAGAATATAAAAGC TATATTTTGAAGACTTGAGTTATTTCAGAAAAAACTACAGCCCTTTTTGTCTTACCTGCCTTTTACTTTCGTGTGGATATGTGAAGCATTGGGTCGGGAA CTAGCTGTAGAACACAACTAAAAACTCATGTCTTTTTTCACAGAATAATGTGCCAGTTTTTTGTAGCAATGTTATTTCTCTTGGAAGCAGAAATGCTTTG TACCAGAGCACCTCCAAACTGCATTGAGGAGAAGTTCCAGAACCATCCCCTTTTTCCATTTTTATATAATTTATAAAGAAAGATTAAAGCCATGTTGACT ATTTTACAGCCACTGGAGTTAACTAACCCTTCCTTGTATCTGTCTTCCCAGGAGAGAATGAAGCAAAACAGGAATTTGGTTTTCTTTTGATGTCCAGTTA CACCATCCATTCTGTTAATTTTGAAAAAATATACCCTCCCTTTAGTTTGTTGGGGGATATAAATTATTCTCAGGAAGAATATAATGAACTGTACAGTTAC TTTGACCTATTAAAAAGGTGTTACCAGTAAAGTTCTTGTTGTAATATCCTTTCTTTTGGTTCTGTTTCTTCAGATGGCTTTTCAGGTGACTTGTCAGATA AGATAACATACAAGAGAGTTCCCAATAATTATCAGGAAGCCTCTCACTAATTAGTTTCTTTTTTTTTATTTAATCTTCTAAACAAGTCAAGGATGTTACC ACAGAAGGGTAGCGTGGCGTAAGTTAACCTTTTCATTCCATCGTCACCTATTAAATGCAGAGCTCAGGATGGAAGTAGCAATGGTCTTCAATGAAAGAAC CTTCCCCTGCGCAGGGTCTCACTATTCCGCTAACTCGGACTCAAAGCATAATTCACTTGTCAAGGAAATTGTTAGCATTTCCCAGGCCCACCCTAGTCTT GCTAGAATCTCTGTGAGCCACAGAGCAGGGAGCAAATAGAACTGAGATCTCCAGCACTAGGGACTCAGACTCCAAAGGGAGGGATTTGAGTGAATTTTTT CCAGGTAAATACATGCTCTTATGTGC

PTK2.au.8.f (forward primer)
PTK2.u.r.53 (reverse primer)
Single exon skipping

Frame 0

VSGYPGSHGITAMAGSIYPGQASLLDQTDSWNH RPQEIAMWQPNVEDSTVLDLRGIGQVLPTHLMEE RLIRQQQEMEEDQRWLEKEERFLIGNQHIYQPV GKPDPAAPPKKPPRPGAPGHLGSLASLSSPADS YNEGVKLQPQEISPPPTANLDRSNDKVYENVTGL VKAVIEMSSKIQPAPPEEYVPMVKEVGLALRTL LATVDETIPLLPASTHREIEMAQKLLNSDLGEL INKMKLAQQYVMTSLQQEYKKQMLTAAHALAVDA KNLLDVIDQARLKMLGQTRPH*ASPRSTSCYPL LKMFSSLPPAARN*PCVLSRQHLQLQLF*MTIW LKNLSHISLTTL*FGFIFCFVFFNHDIQKNPGSK MWRFSKNENYM*AFKHHEEQFMFTLRYVLKGDG QGVTS*FLNYLSCIVEEESMKQRIPGNPRG*EF FCLP*NYYPDWNFCLLEHPSVAIC*SHFTKNIKA IF*RLELFQKKLQPFLSYLPFTFVWICEALGRE LAVEHN*KLMSFFTE*CASFL*QCYFSWKQKCF VPEHLQTALRRSSRTIPFFHFYIIYKERLKPC*L FYSHWS*LTLPCICLPRRE*SKTGIWFSFDVQL HHPFC*F*KNIPSL*FVGGYKLFSGRI**TVQL L*PIKKVLPVKFLL*YPFFWFCFFRWLFR*LVR* DNIQESSQ*LSGSLSLISFFFFI*SSKQVKDVT TEG*RGVS*PFHSIVTY*MQSSGWK*QWSSMKE PSPAQGLTIPLTRTQSIIHLSRKLLAFPRPTLVL LESL*ATEQGANRTEISSTRDSDSKGRDLSEFF PGKYMLLC

Frame 1

FLATLVHMESQPWLAASIQVRHLFWTKQIHGII DLRR*QCGSPMWRTLQYWTCEGLGKCCQPI*WK SV*SDSNRKWKKISAGWKKRKDF*LETNIYISLW VNQILQLHQRNRLALELPVIWEALPASAALLTA TTRVSSFSPRKSAPLLLPTWTGRMIRCTRM*RA W*KLSSRCPVKSSQPHQRSMSLW*RKSAWP*GHY WPLWMRPFPSYQPAPTERLRWHRSY*TLTWVSS STR*NWPSSMS*PASSKSTKSKC*LLLTPWLWM PKTYSMSLTKQD*KCLGRRDHTEPPLGARLATLF *RCSLAFHQQRGINPVSSVASTYSSNFFE*PSG *KIFLI*V*PHFDLGSFFVLFFSIMIFRKIQDP KCGVFLRMKIICKLLSIMKNNLCSH*DTF*RGMA KG*HLNS*TTLAA*WKRRA*SKEFQETQEAENS FVYHRIIIQTGIFVC*NTLQLQYANPTLQRI*K LYFEDLSYFRKNYSPFCLTCLLLSCGYVKHWVGN *L*NTTKNSCLFSQNNVPVFCSNVISLGSRNAL YQSTSKLH*GEVPEPSPFSIFI*FIKKD*SHVD YFTATGVN*PFLVSVFPGENEAKQEFGFLLMSSY TIHSVNFEKIYPPFSLLGDINYSQEEYNELYSY FDLLKRCYQ*SSCCNILSFGSVSSDGFSGDLSD KITYKRVPNNYQEASH*LVSFFLFNLLNKSRMLP QKGSVA*VNLFIPSSPIKCRAQDGSSNGLQ*KN LPLRRVSLFR*LGLKA*FTCQGNC*HFPGPP*S C*NLCEPQSREQIELRSPALGTQTPKGGI*VNFF QVNTCSYV

Frame 2

FWLPWFTWNHSHGWQHLSRSGISFGPNRFMES* TSGDSNVAAQCGGLYSIGPARDWASVANPSDGR ASNPTATGNGRRSALAGKRGKISDWKPTYISAC G*TRSCSSTKETASPWSSRSSGKPCQPQQPC*QL QRGCQASAPGNQPPSYCQPGPVE**GVRECDGP GESCHRDVQ*NPASPTRGVCPYGEGSRLGPEDI IGHCG*DHSPPTSQHPPRD*DGTEAIEL*PG*AH QQDETGPAVCHDQPPARVQKANADCCSRPGCGC QKLTRCH*PSKTENAWADETTLSLP*EHVLLPS FEDVL*PSTSSEELTLCPQSPALTAPTFLNDHLV EKSFSYKFNHTLIWVHFLFCFFQS*YSEKSRIQ NVAFF*E*KLYVSF*AS*RTIYVHIKIRSKGGW PRGDILIPKLP*LHSGRGEHEAKNSRKPKRLRIL LSTIELLSRLEFLFVRTPFSCNMLIPLYKEYKS YILKT*VISEKTTALFVLPAFYFRVDM*SIGSG TSCRTQLKTHVFFHRIMCQFFVAMLFLLEAEMLC TRAPPNCIEEKFQNHPLFPFLYNL*RKIKAMLT ILQPLELTNPSLYLSSQERMKQNRNLVFF*CPV TPSILLILKKYTLPLVCWGI*IILRKNIMNCTVT LTY*KGVTSKVLVVISFLLVLFLQMAFQVTCQI R*HTREFPIIIRKPLTN*FLFFYLIF*TSQGCY HRRVAWRKLTFSFHRHLLNAELRMEVAMVFNERT FPCAGSHYSANSDSKHNSLVKEIVSISQAHPSL ARISVSHRAGSK*N*DLQH*GLRLQREGFE*IF SR*IHALMC

Sequence PTK2.lAug05

TTGTCTCTCTAGCACAATCCTGGAGGAAGAGAAGGCTCAGCAAGAAGAGCGCATGAGGATGGAGTCCAGAAGACAGGCCACAGTGTCCTGGGACTCCGGA GGGTCTGATGAAGCACCGCCCAAGCCCAGCAGACCGGGTTATCCCAGTCCGAGGTCCAGCGAAGGATTTTATCCCAGCCCACAGCACATGGTACAAACCA ATCATTACCAGGTTTCTGGCTACCCTGGTTCACATGGAATCACAGCCATGGCTGGCAGCATCTATCCAGGTCAGGCATCTCTTTTGGACCAAACAGATTC ATGGAATCATAGACCTCAGGAGATAGCAATGTGGCAGCCCAATGTGGAGGACTCTACAGTATTGGACCTGCGAGGGATTGGGCAAGTGTTGCCAACCCAT CTGATGGAAGAGCGTCTAATCCGACAGCAACAGGAAATGGAAGAAGATCAGCGCTGGCTGGAAAAAGAGGAAAGATTTCTGAAACCTGATGTGAGACTCT CTCGAGGCAGTATTGACAGGGAGGATGGAAGTCTTCAGGGTCCGATTGGAAACCAACATATATATCAGCCTGTGGGTAAACCAGATCCTGCAGCTCCACC AAAGAAACCGCCTCGCCCTGGAGCTCCCGGTCATCTGGGAAGCCTTGCCAGCCTCAGCAGCCCTGCTGACAGCTACAACGAGGGTGTCAAGCTTCAGCCC CAGGAAATCAGCCCCCCTCCTACTGCCAACCTGGACCGGTCGAATGATAAGGTGTACGAGAATGTGACGGGCCTGGTGAAAGCTGTCATCGAGATGTCCA GTAAAATCCAGCCAGCCCCACCAGAGGAGTATGTCCCTATGGTGAAGGAAGTCGGCTTGGCCCTGAGGACATTATTGGCCACTGTGGATGAGACCATTCC CCTCCTACCAGCCAGCACCCACCGAGAGATTGAGATGGCACAGAAGCTATTGAACTCTGACCTGGGTGAGCTCATCAACAAGATGAAACTGGCCCAGCAG TATGTCATGACCAGCCTCCAGCAAGAGTACAAAAAGCAAATGCTGACTGCTGCTCACGCCCTGGCTGTGGATGCCAAAAACTTACTCGATGTCATTGACC AAGCAAGACTGAAAATGCTTGGGCAGACGAGACCACACTGAGCCTCCCCTAGGAGCACGTCTTGCTACCCTCTTTTGAAGATGTTCTCTAGCCTTCCACC AGCAGCGAGGAATTAACCCTGTGTCCTCAGTCGCCAGCACTTACAGCTCCAACTTTTTTGAATGACCATCTGGTTGAAAAATCTTTCTCATATAAGTTTA ACCACACTTTGATTTGGGTTCATTTTTTGTTTTGTTTTTTTCAATCATGATATTCAGAAAAATCCAGGATCCAAAATGTGGCGTTTTTCTAAGAATGAAA ATTATATGTAAGCTTTTAAGCATCATGAAGAACAATTTATGTTCACATTAAGATACGTTCTAAAGGGGGATGGCCAAGGGGTGACATCTTAATTCCTAAA CTACCTTAGCTGCATAGTGGAAGAGGAGAGCATGAAGCAAAGAATTCCAGGAAACCCAAGAGGCTGAGAATTCTTTTGTCTACCATAGAATTATTATCCA GACTGGAATTTTTGTTTGTTAGAACACCCTTCAGTTGCAATATGCTAATCCCACTTTACAAAGAATATAAAAGCTATATTTTGAAGACTTGAGTTATTTC AGAAAAAACTACAGCCCTTTTTGTCTTACCTGCCTTTTACTTTCGTGTGGATATGTGAAGCATTGGGTCGGGAACTAGCTGTAGAACACAACTAAAAACT CATGTCTTTTTTCACAGAATAATGTGCCAGTTTTTTGTAGCAATGTTATTTCTCTTGGAAGCAGAAATGCTTTGTACCAGAGCACCTCCAAACTGCATTG AGGAGAAGTTCCAGAACCATCCCCTTTTTCCATTTTTATATAATTTATAAAGAAAGATTAAAGCCATGTTGACTATTTTACAGCCACTGGAGTTAACTAA CCCTTCCTTGTATCTGTCTTCCCAGGAGAGAATGAAGCAAAACAGGAATTTGGTTTTCTTTTGATGTCCAGTTACACCATCCATTCTGTTAATTTTGAAA AAATATACCCTCCCTTTAGTTTGTTGGGGGATATAAATTATTCTCAGGAAGAATATAATGAACTGTACAGTTACTTTGACCTATTAAAAAGGTGTTACCA GTAAAGTTCTTGTTGTAATATCCTTTCTTTTGGTTCTGTTTCTTCAGATGGCTTTTCAGGTGACTTGTCAGATAAGATAACATACAAGAGAGTTCCCAAT AATTATCAGGAAGCCTCTCACTAATTAGTTTCTTTTTTTTTATTTAATCTTCTAAACAAGTCAAGGATGTTACCACAGAAGGGTAGCGTGGCGTAAGTTA ACCTTTTCATTCCATCGTCACCTATTAAATGCAGAGCTCAGGATGGAAGTAGCAATGGTCTTCAATGAAAGAACCTTCCCCTGCGCAGGGTCTCACTATT CCGCTAACTCGGACTCAAAGCATAATTCACTTGTCAAGGAAATTGTTAGCATTTCCCAGGCCCACCCTAGTCTTGCTAGAATCTCTGTGAGCCACAGAGC AGGGAGCAAATAGAACTGAGATCTCCAGCACTAGGGACTCAGACTCCAAAGGGAGGGATTTGAGTGAATTTTTTCCAGGTAAATACATGCTCTTATGTGC

PTK2.au.8.f (forward primer)
PTK2.u.r.53 (reverse primer)
Single exon skipping

Frame 0

LSL*HNPGGREGSARRAHEDGVQKTGHSVLGLR RV**STAQAQQTGLSQSEVQRRILSQPTAHGTNQ SLPGFWLPWFTWNHSHGWQHLSRSGISFGPNRF MES*TSGDSNVAAQCGGLYSIGPARDWASVANP SDGRASNPTATGNGRRSALAGKRGKISET*CETL SRQY*QGGWKSSGSDWKPTYISACG*TRSCSST KETASPWSSRSSGKPCQPQQPC*QLQRGCQASA PGNQPPSYCQPGPVE**GVRECDGPGESCHRDVQ *NPASPTRGVCPYGEGSRLGPEDIIGHCG*DHS PPTSQHPPRD*DGTEAIEL*PG*AHQQDETGPA VCHDQPPARVQKANADCCSRPGCGCQKLTRCH*P SKTENAWADETTLSLP*EHVLLPSFEDVL*PST SSEELTLCPQSPALTAPTFLNDHLVEKSFSYKF NHTLIWVHFLFCFFQS*YSEKSRIQNVAFF*E*K LYVSF*AS*RTIYVHIKIRSKGGWPRGDILIPK LP*LHSGRGEHEAKNSRKPKRLRILLSTIELLS RLEFLFVRTPFSCNMLIPLYKEYKSYILKT*VIS EKTTALFVLPAFYFRVDM*SIGSGTSCRTQLKT HVFFHRIMCQFFVAMLFLLEAEMLCTRAPPNCI EEKFQNHPLFPFLYNL*RKIKAMLTILQPLELTN PSLYLSSQERMKQNRNLVFF*CPVTPSILLILK KYTLPLVCWGI*IILRKNIMNCTVTLTY*KGVT SKVLVVISFLLVLFLQMAFQVTCQIR*HTREFPI IIRKPLTN*FLFFYLIF*TSQGCYHRRVAWRKL TFSFHRHLLNAELRMEVAMVFNERTFPCAGSHY SANSDSKHNSLVKEIVSISQAHPSLARISVSHRA GSK*N*DLQH*GLRLQREGFE*IFSR*IHALMC

Frame 1

CLSSTILEEEKAQQEERMRMESRRQATVSWDSG GSDEAPPKPSRPGYPSPRSSEGFYPSPQHMVQT NHYQVSGYPGSHGITAMAGSIYPGQASLLDQTDS WNHRPQEIAMWQPNVEDSTVLDLRGIGQVLPTH LMEERLIRQQQEMEEDQRWLEKEERFLKPDVRL SRGSIDREDGSLQGPIGNQHIYQPVGKPDPAAPP KKPPRPGAPGHLGSLASLSSPADSYNEGVKLQP QEISPPPTANLDRSNDKVYENVTGLVKAVIEMS SKIQPAPPEEYVPMVKEVGLALRTLLATVDETIP LLPASTHREIEMAQKLLNSDLGELINKMKLAQQ YVMTSLQQEYKKQMLTAAHALAVDAKNLLDVID QARLKMLGQTRPH*ASPRSTSCYPLLKMFSSLPP AARN*PCVLSRQHLQLQLF*MTIWLKNLSHISL TTL*FGFIFCFVFFNHDIQKNPGSKMWRFSKNE NYM*AFKHHEEQFMFTLRYVLKGDGQGVTS*FLN YLSCIVEEESMKQRIPGNPRG*EFFCLP*NYYP DWNFCLLEHPSVAIC*SHFTKNIKAIF*RLELF QKKLQPFLSYLPFTFVWICEALGRELAVEHN*KL MSFFTE*CASFL*QCYFSWKQKCFVPEHLQTAL RRSSRTIPFFHFYIIYKERLKPC*LFYSHWS*L TLPCICLPRRE*SKTGIWFSFDVQLHHPFC*F*K NIPSL*FVGGYKLFSGRI**TVQLL*PIKKVLP VKFLL*YPFFWFCFFRWLFR*LVR*DNIQESSQ *LSGSLSLISFFFFI*SSKQVKDVTTEG*RGVS* PFHSIVTY*MQSSGWK*QWSSMKEPSPAQGLTI PLTRTQSIIHLSRKLLAFPRPTLVLLESL*ATE QGANRTEISSTRDSDSKGRDLSEFFPGKYMLLC

Frame 2

VSLAQSWRKRRLSKKSA*GWSPEDRPQCPGTPE GLMKHRPSPADRVIPVRGPAKDFIPAHSTWYKP IITRFLATLVHMESQPWLAASIQVRHLFWTKQI HGIIDLRR*QCGSPMWRTLQYWTCEGLGKCCQPI *WKSV*SDSNRKWKKISAGWKKRKDF*NLM*DS LEAVLTGRMEVFRVRLETNIYISLWVNQILQLH QRNRLALELPVIWEALPASAALLTATTRVSSFSP RKSAPLLLPTWTGRMIRCTRM*RAW*KLSSRCP VKSSQPHQRSMSLW*RKSAWP*GHYWPLWMRPF PSYQPAPTERLRWHRSY*TLTWVSSSTR*NWPSS MS*PASSKSTKSKC*LLLTPWLWMPKTYSMSLT KQD*KCLGRRDHTEPPLGARLATLF*RCSLAFH QQRGINPVSSVASTYSSNFFE*PSG*KIFLI*V* PHFDLGSFFVLFFSIMIFRKIQDPKCGVFLRMK IICKLLSIMKNNLCSH*DTF*RGMAKG*HLNS* TTLAA*WKRRA*SKEFQETQEAENSFVYHRIIIQ TGIFVC*NTLQLQYANPTLQRI*KLYFEDLSYF RKNYSPFCLTCLLLSCGYVKHWVGN*L*NTTKN SCLFSQNNVPVFCSNVISLGSRNALYQSTSKLH* GEVPEPSPFSIFI*FIKKD*SHVDYFTATGVN* PFLVSVFPGENEAKQEFGFLLMSSYTIHSVNFE KIYPPFSLLGDINYSQEEYNELYSYFDLLKRCYQ *SSCCNILSFGSVSSDGFSGDLSDKITYKRVPN NYQEASH*LVSFFLFNLLNKSRMLPQKGSVA*V NLFIPSSPIKCRAQDGSSNGLQ*KNLPLRRVSLF R*LGLKA*FTCQGNC*HFPGPP*SC*NLCEPQS REQIELRSPALGTQTPKGGI*VNFFQVNTCSYV

Sequence PTK2.kAug05

GAGTAATTTTTGGGTGGTTGTTTACTGCACACTATCTACCAGGAGCCTAGAAACTTTGAATGAGTGTCATTATTAGAACTCTAGAGTTGTAGGAATGTAG GAAGAGCTGTTTGCCACAGTTAGGACGAGGGTTTCAAACTGTCTGCTGAGCCCTGGGGCATCTGCGTGGAATGGGAAACTAGCAAAGTCTCGCTTTGGCT GCATTCTGAGGGGTTTATGTGATGGCAAACAGCCAAGATGAAGAAGCTGCCTAGCAGTCGCTGCAGCGGCTGCAGCGTTATGTGGGACTTCAGATAGCTT CTGAGTAGTCAAGCAGTGCACAATCCTGGAGGAAGAGAAGGCTCAGCAAGAAGAGCGCATGAGGATGGAGTCCAGAAGACAGGCCACAGTGTCCTGGGAC TCCGGAGGGTCTGATGAAGCACCGCCCAAGCCCAGCAGACCGGGTTATCCCAGTCCGAGGTCCAGCGAAGGATTTTATCCCAGCCCACAGCACATGGTAC AAACCAATCATTACCAGGTTTCTGGCTACCCTGGTTCACATGGAATCACAGCCATGGCTGGCAGCATCTATCCAGGTCAGGCATCTCTTTTGGACCAAAC AGATTCATGGAATCATAGACCTCAGGAGATAGCAATGTGGCAGCCCAATGTGGAGGACTCTACAGTATTGGACCTGCGAGGGATTGGGCAAGTGTTGCCA ACCCATCTGATGGAAGAGCGTCTAATCCGACAGCAACAGGAAATGGAAGAAGATCAGCGCTGGCTGGAAAAAGAGGAAAGATTTCTGAAACCTGATGTGA GACTCTCTCGAGGCAGTATTGACAGGGAGGATGGAAGTCTTCAGGGTCCGATTGGAAACCAACATATATATCAGCCTGTGGGTAAACCAGATCCTGCAGC TCCACCAAAGAAACCGCCTCGCCCTGGAGCTCCCGGTCATCTGGGAAGCCTTGCCAGCCTCAGCAGCCCTGCTGACAGCTACAACGAGGGTGTCAAGCTT CAGCCCCAGGAAATCAGCCCCCCTCCTACTGCCAACCTGGACCGGTCGAATGATAAGGTGTACGAGAATGTGACGGGCCTGGTGAAAGCTGTCATCGAGA TGTCCAGTAAAATCCAGCCAGCCCCACCAGAGGAGTATGTCCCTATGGTGAAGATTGAGATGGCACAGAAGCTATTGAACTCTGACCTGGGTGAGCTCAT CAACAAGATGAAACTGGCCCAGCAGTATGTCATGACCAGCCTCCAGCAAGAGTACAAAAAGCAAATGCTGACTGCTGCTCACGCCCTGGCTGTGGATGCC AAAAACTTACTCGATGTCATTGACCAAGCAAGACTGAAAATGCTTGGGCAGACGAGACCACACTGAGCCTCCCCTAGGAGCACGTCTTGCTACCCTCTTT TGAAGATGTTCTCTAGCCTTCCACCAGCAGCGAGGAATTAACCCTGTGTCCTCAGTCGCCAGCACTTACAGCTCCAACTTTTTTGAATGACCATCTGGTT GAAAAATCTTTCTCATATAAGTTTAACCACACTTTGATTTGGGTTCATTTTTTGTTTTGTTTTTTTCAATCATGATATTCAGAAAAATCCAGGATCCAAA ATGTGGCGTTTTTCTAAGAATGAAAATTATATGTAAGCTTTTAAGCATCATGAAGAACAATTTATGTTCACATTAAGATACGTTCTAAAGGGGGATGGCC AAGGGGTGACATCTTAATTCCTAAACTACCTTAGCTGCATAGTGGAAGAGGAGAGCATGAAGCAAAGAATTCCAGGAAACCCAAGAGGCTGAGAATTCTT TTGTCTACCATAGAATTATTATCCAGACTGGAATTTTTGTTTGTTAGAACACCCTTCAGTTGCAATATGCTAATCCCACTTTACAAAGAATATAAAAGCT ATATTTTGAAGACTTGAGTTATTTCAGAAAAAACTACAGCCCTTTTTGTCTTACCTGCCTTTTACTTTCGTGTGGATATGTGAAGCATTGGGTCGGGAAC TAGCTGTAGAACACAACTAAAAACTCATGTCTTTTTTCACAGAATAATGTGCCAGTTTTTTGTAGCAATGTTATTTCTCTTGGAAGCAGAAATGCTTTGT ACCAGAGCACCTCCAAACTGCATTGAGGAGAAGTTCCAGAACCATCCCCTTTTTCCATTTTTATATAATTTATAAAGAAAGATTAAAGCCATGTTGACTA TTTTACAGCCACTGGAGTTAACTAACCCTTCCTTGTATCTGTCTTCCCAGGAGAGAATGAAGCAAAACAGGAATTTGGTTTTCTTTTGATGTCCAGTTAC ACCATCCATTCTGTTAATTTTGAAAAAATATACCCTCCCTTTAGTTTGTTGGGGGATATAAATTATTCTCAGGAAGAATATAATGAACTGTACAGTTACT TTGACCTATTAAAAAGGTGTTACCAGTAAAGTTCTTGTTGTAATATCCTTTCTTTTGGTTCTGTTTCTTCAGATGGCTTTTCAGGTGACTTGTCAGATAA GATAACATACAAGAGAGTTCCCAATAATTATCAGGAAGCCTCTCACTAATTAGTTTCTTTTTTTTTATTTAATCTTCTAAACAAGTCAAGGATGTTACCA CAGAAGGGTAGCGTGGCGTAAGTTAACCTTTTCATTCCATCGTCACCTATTAAATGCAGAGCTCAGGATGGAAGTAGCAATGGTCTTCAATGAAAGAACC TTCCCCTGCGCAGGGTCTCACTATTCCGCTAACTCGGACTCAAAGCATAATTCACTTGTCAAGGAAATTGTTAGCATTTCCCAGGCCCACCCTAGTCTTG CTAGAATCTCTGTGAGCCACAGAGCAGGGAGCAAATAGAACTGAGATCTCCAGCACTAGGGACTCAGACTCCAAAGGGAGGGATTTGAGTGAATTTTTTC CAGGTAAATACATGCTCTTATGTGC

PTK2.au.8.f (forward primer)
PTK2.u.r.53 (reverse primer)
Single exon skipping

Frame 0

E*FLGGCLLHTIYQEPRNFE*VSLLEL*SCRNV GRAVCHS*DEGFKLSAEPWGICVEWETSKVSLWL HSEGFM*WQTAKMKKLPSSRCSGCSVMWDFR*L LSSQAVHNPGGREGSARRAHEDGVQKTGHSVLG LRRV**STAQAQQTGLSQSEVQRRILSQPTAHGT NQSLPGFWLPWFTWNHSHGWQHLSRSGISFGPN RFMES*TSGDSNVAAQCGGLYSIGPARDWASVA NPSDGRASNPTATGNGRRSALAGKRGKISET*CE TLSRQY*QGGWKSSGSDWKPTYISACG*TRSCS STKETASPWSSRSSGKPCQPQQPC*QLQRGCQA SAPGNQPPSYCQPGPVE**GVRECDGPGESCHRD VQ*NPASPTRGVCPYGED*DGTEAIEL*PG*AH QQDETGPAVCHDQPPARVQKANADCCSRPGCGC QKLTRCH*PSKTENAWADETTLSLP*EHVLLPSF EDVL*PSTSSEELTLCPQSPALTAPTFLNDHLV EKSFSYKFNHTLIWVHFLFCFFQS*YSEKSRIQ NVAFF*E*KLYVSF*AS*RTIYVHIKIRSKGGWP RGDILIPKLP*LHSGRGEHEAKNSRKPKRLRIL LSTIELLSRLEFLFVRTPFSCNMLIPLYKEYKS YILKT*VISEKTTALFVLPAFYFRVDM*SIGSGT SCRTQLKTHVFFHRIMCQFFVAMLFLLEAEMLC TRAPPNCIEEKFQNHPLFPFLYNL*RKIKAMLT ILQPLELTNPSLYLSSQERMKQNRNLVFF*CPVT PSILLILKKYTLPLVCWGI*IILRKNIMNCTVT LTY*KGVTSKVLVVISFLLVLFLQMAFQVTCQI R*HTREFPIIIRKPLTN*FLFFYLIF*TSQGCYH RRVAWRKLTFSFHRHLLNAELRMEVAMVFNERT FPCAGSHYSANSDSKHNSLVKEIVSISQAHPSL ARISVSHRAGSK*N*DLQH*GLRLQREGFE*IFS R*IHALMC

Frame 1

SNFWVVVYCTLSTRSLETLNECHY*NSRVVGM* EELFATVRTRVSNCLLSPGASAWNGKLAKSRFG CILRGLCDGKQPR*RSCLAVAAAAAALCGTSDSF *VVKQCTILEEEKAQQEERMRMESRRQATVSWD SGGSDEAPPKPSRPGYPSPRSSEGFYPSPQHMV QTNHYQVSGYPGSHGITAMAGSIYPGQASLLDQT DSWNHRPQEIAMWQPNVEDSTVLDLRGIGQVLP THLMEERLIRQQQEMEEDQRWLEKEERFLKPDV RLSRGSIDREDGSLQGPIGNQHIYQPVGKPDPAA PPKKPPRPGAPGHLGSLASLSSPADSYNEGVKL QPQEISPPPTANLDRSNDKVYENVTGLVKAVIE MSSKIQPAPPEEYVPMVKIEMAQKLLNSDLGELI NKMKLAQQYVMTSLQQEYKKQMLTAAHALAVDA KNLLDVIDQARLKMLGQTRPH*ASPRSTSCYPL LKMFSSLPPAARN*PCVLSRQHLQLQLF*MTIWL KNLSHISLTTL*FGFIFCFVFFNHDIQKNPGSK MWRFSKNENYM*AFKHHEEQFMFTLRYVLKGDG QGVTS*FLNYLSCIVEEESMKQRIPGNPRG*EFF CLP*NYYPDWNFCLLEHPSVAIC*SHFTKNIKA IF*RLELFQKKLQPFLSYLPFTFVWICEALGRE LAVEHN*KLMSFFTE*CASFL*QCYFSWKQKCFV PEHLQTALRRSSRTIPFFHFYIIYKERLKPC*L FYSHWS*LTLPCICLPRRE*SKTGIWFSFDVQL HHPFC*F*KNIPSL*FVGGYKLFSGRI**TVQLL *PIKKVLPVKFLL*YPFFWFCFFRWLFR*LVR* DNIQESSQ*LSGSLSLISFFFFI*SSKQVKDVT TEG*RGVS*PFHSIVTY*MQSSGWK*QWSSMKEP SPAQGLTIPLTRTQSIIHLSRKLLAFPRPTLVL LESL*ATEQGANRTEISSTRDSDSKGRDLSEFF PGKYMLLC

Frame 2

VIFGWLFTAHYLPGA*KL*MSVIIRTLEL*ECR KSCLPQLGRGFQTVC*ALGHLRGMGN*QSLALA AF*GVYVMANSQDEEAA*QSLQRLQRYVGLQIA SE*SSSAQSWRKRRLSKKSA*GWSPEDRPQCPGT PEGLMKHRPSPADRVIPVRGPAKDFIPAHSTWY KPIITRFLATLVHMESQPWLAASIQVRHLFWTK QIHGIIDLRR*QCGSPMWRTLQYWTCEGLGKCCQ PI*WKSV*SDSNRKWKKISAGWKKRKDF*NLM* DSLEAVLTGRMEVFRVRLETNIYISLWVNQILQ LHQRNRLALELPVIWEALPASAALLTATTRVSSF SPRKSAPLLLPTWTGRMIRCTRM*RAW*KLSSR CPVKSSQPHQRSMSLW*RLRWHRSY*TLTWVSS STR*NWPSSMS*PASSKSTKSKC*LLLTPWLWMP KTYSMSLTKQD*KCLGRRDHTEPPLGARLATLF *RCSLAFHQQRGINPVSSVASTYSSNFFE*PSG *KIFLI*V*PHFDLGSFFVLFFSIMIFRKIQDPK CGVFLRMKIICKLLSIMKNNLCSH*DTF*RGMA KG*HLNS*TTLAA*WKRRA*SKEFQETQEAENS FVYHRIIIQTGIFVC*NTLQLQYANPTLQRI*KL YFEDLSYFRKNYSPFCLTCLLLSCGYVKHWVGN *L*NTTKNSCLFSQNNVPVFCSNVISLGSRNAL YQSTSKLH*GEVPEPSPFSIFI*FIKKD*SHVDY FTATGVN*PFLVSVFPGENEAKQEFGFLLMSSY TIHSVNFEKIYPPFSLLGDINYSQEEYNELYSY FDLLKRCYQ*SSCCNILSFGSVSSDGFSGDLSDK ITYKRVPNNYQEASH*LVSFFLFNLLNKSRMLP QKGSVA*VNLFIPSSPIKCRAQDGSSNGLQ*KN LPLRRVSLFR*LGLKA*FTCQGNC*HFPGPP*SC *NLCEPQSREQIELRSPALGTQTPKGGI*VNFF QVNTCSYV

Sequence PTK2.iAug05

GAGTAATAGTGTTTTAAGAATATTTCAGCGTAGTAGAGGATTTAAGCAGATCGCTTTGCCTACTTTAATTATATGAAATACCAGGAGGTCAGATGTTTAA CCTCTTTCAACATATCTGTTTCTTTTCCTGCAGAAACAGATGATTATGCTGAGATTATAGATGAAGAAGATACTTACACCATGCCCTCAACCAGGGATTA TGAGATTCAAAGAGAAAGAATAGAACTTGGACGATGTATTGGAGAAGGCCAATTTGGAGATGTACATCAAGGCATTTATATGAGTCCAGAGAATCCAGCT TTGGCGGTTGCAATTAAAACATGTAAAAACTGTACTTCGGACAGCGTGAGAGAGAAATTTCTTCAAGAAGCCTTAACAATGCGTCAGTTTGACCATCCTC ATATTGTGAAGCTGATTGGAGTCATCACAGAGAATCCTGTCTGGATAATCATGGAGCTGTGCACACTTGGAGAGCTGAGGTCATTTTTGCAAGTAAGGAA ATACAGTTTGGATCTAGCATCTTTGATCCTGTATGCCTATCAGCTTAGTACAGCTCTTGCATATCTAGAGAGCAAAAGATTTGTACACAGGGACATTGCT GCTCGGAATGTTCTGGTGTCCTCAAATGATTGTGTAAAATTAGGAGACTTTGGATTATCCCGATATATGGAAGATAGTACTTACTACAAAGCTTCCAAAG GAAAATTGCCTATTAAATGGATGGCTCCAGAGTCAATCAATTTTCGACGTTTTACCTCAGCTAGTGACGTATGGATGTTTGGTGTGTGTATGTGGGAGAT ACTGATGCATGGTGTGAAGCCTTTTCAAGGAGTGAAGAACAATGATGTAATCGGTCGAATTGAAAATGGGGAAAGATTACCAATGCCTCCAAATTGTCCT CCTACCCTCTACAGCCTTATGACGAAATGCTGGGCCTATGACCCCAGCAGGCGGCCCAGGTTTACTGAACTTAAAGCTCAGCTCAGCACAATCCTGGAGG AAGAGAAGGCTCAGCAAGAAGAGCGCATGAGGATGGAGTCCAGAAGACAGGCCACAGTGTCCTGGGACTCCGGAGGGTCTGATGAAGCACCGCCCAAGCC CAGCAGACCGGGTTATCCCAGTCCGAGGTCCAGCGAAGGATTTTATCCCAGCCCACAGCACATGGTACAAACCAATCATTACCAGGTTTCTGGCTACCCT GGTTCACATGGAATCACAGCCATGGCTGGCAGCATCTATCCAGGTCAGGCATCTCTTTTGGACCAAACAGATTCATGGAATCATAGACCTCAGGAGATAG CAATGTGGCAGCCCAATGTGGAGGACTCTACAGTATTGGACCTGCGAGGGATTGGGCAAGTGTTGCCAACCCATCTGATGGAAGAGCGTCTAATCCGACA GCAACAGGAAATGGAAGAAGATCAGCGCTGGCTGGAAAAAGAGGAAAGATTTCTGAAACCTGATGTGAGACTCTCTCGAGGCAGTATTGACAGGGAGGAT GGAAGTCTTCAGGGTCCGATTGGAAACCAACATATATATCAGCCTGTGGGTAAACCAGATCCTGCAGCTCCACCAAAGAAACCGCCTCGCCCTGGAGCTC CCGGTCATCTGGGAAGCCTTGCCAGCCTCAGCAGCCCTGCTGACAGCTACAACGAGGGTGTCAAGCTTCAGCCCCAGGAAATCAGCCCCCCTCCTACTGC CAACCTGGACCGGTCGAATGATAAGGTGTACGAGAATGTGACGGGCCTGGTGAAAGCTGTCATCGAGATGTCCAGTAAAATCCAGCCAGCCCCACCAGAG GAGTATGTCCCTATGGTGAAGGAAGTCGGCTTGGCCCTGAGGACATTATTGGCCACTGTGGATGAGACCATTCCCCTCCTACCAGCCAGCACCCACCGAG AGATTGAGATGGCACAGAAGCTATTGAACTCTGACCTGGGTGAGCTCATCAACAAGATGAAACTGGCCCAGCAGTATGTCATGACCAGCCTCCAGCAAGA GTACAAAAAGCAAATGCTGACTGCTGCTCACGCCCTGGCTGTGGATGCCAAAAACTTACTCGATGTCATTGACCAAGCAAGACTGAAAATGCTTGGGCAG ACGAGACCACACTGAGCCTCCCCTAGGAGCACGTCTTGCTACCCTCTTTTGAAGATGTTCTCTAGCCTTCCACCAGCAGCGAGGAATTAACCCTGTGTCC TCAGTCGCCAGCACTTACAGCTCCAACTTTTTTGAATGACCATCTGGTTGAAAAATCTTTCTCATATAAGTTTAACCACACTTTGATTTGGGTTCATTTT TTGTTTTGTTTTTTTCAATCATGATATTCAGAAAAATCCAGGATCCAAAATGTGGCGTTTTTCTAAGAATGAAAATTATATGTAAGCTTTTAAGCATCAT GAAGAACAATTTATGTTCACATTAAGATACGTTCTAAAGGGGGATGGCCAAGGGGTGACATCTTAATTCCTAAACTACCTTAGCTGCATAGTGGAAGAGG AGAGCATGAAGCAAAGAATTCCAGGAAACCCAAGAGGCTGAGAATTCTTTTGTCTACCATAGAATTATTATCCAGACTGGAATTTTTGTTTGTTAGAACA CCCTTCAGTTGCAATATGCTAATCCCACTTTACAAAGAATATAAAAGCTATATTTTGAAGACTTGAGTTATTTCAGAAAAAACTACAGCCCTTTTTGTCT TACCTGCCTTTTACTTTCGTGTGGATATGTGAAGCATTGGGTCGGGAACTAGCTGTAGAACACAACTAAAAACTCATGTCTTTTTTCACAGAATAATGTG CCAGTTTTTTGTAGCAATGTTATTTCTCTTGGAAGCAGAAATGCTTTGTACCAGAGCACCTCCAAACTGCATTGAGGAGAAGTTCCAGAACCATCCCCTT TTTCCATTTTTATATAATTTATAAAGAAAGATTAAAGCCATGTTGACTATTTTACAGCCACTGGAGTTAACTAACCCTTCCTTGTATCTGTCTTCCCAGG AGAGAATGAAGCAAAACAGGAATTTGGTTTTCTTTTGATGTCCAGTTACACCATCCATTCTGTTAATTTTGAAAAAATATACCCTCCCTTTAGTTTGTTG GGGGATATAAATTATTCTCAGGAAGAATATAATGAACTGTACAGTTACTTTGACCTATTAAAAAGGTGTTACCAGTAAAGTTCTTGTTGTAATATCCTTT CTTTTGGTTCTGTTTCTTCAGATGGCTTTTCAGGTGACTTGTCAGATAAGATAACATACAAGAGAGTTCCCAATAATTATCAGGAAGCCTCTCACTAATT AGTTTCTTTTTTTTTATTTAATCTTCTAAACAAGTCAAGGATGTTACCACAGAAGGGTAGCGTGGCGTAAGTTAACCTTTTCATTCCATCGTCACCTATT AAATGCAGAGCTCAGGATGGAAGTAGCAATGGTCTTCAATGAAAGAACCTTCCCCTGCGCAGGGTCTCACTATTCCGCTAACTCGGACTCAAAGCATAAT TCACTTGTCAAGGAAATTGTTAGCATTTCCCAGGCCCACCCTAGTCTTGCTAGAATCTCTGTGAGCCACAGAGCAGGGAGCAAATAGAACTGAGATCTCC AGCACTAGGGACTCAGACTCCAAAGGGAGGGATTTGAGTGAATTTTTTCCAGGTAAATACATGCTCTTATGTGC

PTK2.au.8.f (forward primer)
PTK2.u.r.53 (reverse primer)
Single exon skipping

Frame 0

E**CFKNISA**RI*ADRFAYFNYMKYQEVRCL TSFNISVSFPAETDDYAEIIDEEDTYTMPSTRDY EIQRERIELGRCIGEGQFGDVHQGIYMSPENPA LAVAIKTCKNCTSDSVREKFLQEALTMRQFDHP HIVKLIGVITENPVWIIMELCTLGELRSFLQVRK YSLDLASLILYAYQLSTALAYLESKRFVHRDIA ARNVLVSSNDCVKLGDFGLSRYMEDSTYYKASK GKLPIKWMAPESINFRRFTSASDVWMFGVCMWEI LMHGVKPFQGVKNNDVIGRIENGERLPMPPNCP PTLYSLMTKCWAYDPSRRPRFTELKAQLSTILE EEKAQQEERMRMESRRQATVSWDSGGSDEAPPKP SRPGYPSPRSSEGFYPSPQHMVQTNHYQVSGYP GSHGITAMAGSIYPGQASLLDQTDSWNHRPQEI AMWQPNVEDSTVLDLRGIGQVLPTHLMEERLIRQ QQEMEEDQRWLEKEERFLKPDVRLSRGSIDRED GSLQGPIGNQHIYQPVGKPDPAAPPKKPPRPGA PGHLGSLASLSSPADSYNEGVKLQPQEISPPPTA NLDRSNDKVYENVTGLVKAVIEMSSKIQPAPPE EYVPMVKEVGLALRTLLATVDETIPLLPASTHR EIEMAQKLLNSDLGELINKMKLAQQYVMTSLQQE YKKQMLTAAHALAVDAKNLLDVIDQARLKMLGQ TRPH*ASPRSTSCYPLLKMFSSLPPAARN*PCV LSRQHLQLQLF*MTIWLKNLSHISLTTL*FGFIF CFVFFNHDIQKNPGSKMWRFSKNENYM*AFKHH EEQFMFTLRYVLKGDGQGVTS*FLNYLSCIVEE ESMKQRIPGNPRG*EFFCLP*NYYPDWNFCLLEH PSVAIC*SHFTKNIKAIF*RLELFQKKLQPFLS YLPFTFVWICEALGRELAVEHN*KLMSFFTE*C ASFL*QCYFSWKQKCFVPEHLQTALRRSSRTIPF FHFYIIYKERLKPC*LFYSHWS*LTLPCICLPR RE*SKTGIWFSFDVQLHHPFC*F*KNIPSL*FV GGYKLFSGRI**TVQLL*PIKKVLPVKFLL*YPF FWFCFFRWLFR*LVR*DNIQESSQ*LSGSLSLI SFFFFI*SSKQVKDVTTEG*RGVS*PFHSIVTY *MQSSGWK*QWSSMKEPSPAQGLTIPLTRTQSII HLSRKLLAFPRPTLVLLESL*ATEQGANRTEIS STRDSDSKGRDLSEFFPGKYMLLC

Frame 1

SNSVLRIFQRSRGFKQIALPTLII*NTRRSDV* PLSTYLFLFLQKQMIMLRL*MKKILTPCPQPGI MRFKEKE*NLDDVLEKANLEMYIKAFI*VQRIQL WRLQLKHVKTVLRTA*ERNFFKKP*QCVSLTIL IL*S*LESSQRILSG*SWSCAHLES*GHFCK*G NTVWI*HL*SCMPISLVQLLHI*RAKDLYTGTLL LGMFWCPQMIV*N*ETLDYPDIWKIVLTTKLPK ENCLLNGWLQSQSIFDVLPQLVTYGCLVCVCGR Y*CMV*SLFKE*RTMM*SVELKMGKDYQCLQIVL LPSTAL*RNAGPMTPAGGPGLLNLKLSSAQSWR KRRLSKKSA*GWSPEDRPQCPGTPEGLMKHRPS PADRVIPVRGPAKDFIPAHSTWYKPIITRFLATL VHMESQPWLAASIQVRHLFWTKQIHGIIDLRR* QCGSPMWRTLQYWTCEGLGKCCQPI*WKSV*SD SNRKWKKISAGWKKRKDF*NLM*DSLEAVLTGRM EVFRVRLETNIYISLWVNQILQLHQRNRLALEL PVIWEALPASAALLTATTRVSSFSPRKSAPLLL PTWTGRMIRCTRM*RAW*KLSSRCPVKSSQPHQR SMSLW*RKSAWP*GHYWPLWMRPFPSYQPAPTE RLRWHRSY*TLTWVSSSTR*NWPSSMS*PASSK STKSKC*LLLTPWLWMPKTYSMSLTKQD*KCLGR RDHTEPPLGARLATLF*RCSLAFHQQRGINPVS SVASTYSSNFFE*PSG*KIFLI*V*PHFDLGSF FVLFFSIMIFRKIQDPKCGVFLRMKIICKLLSIM KNNLCSH*DTF*RGMAKG*HLNS*TTLAA*WKR RA*SKEFQETQEAENSFVYHRIIIQTGIFVC*N TLQLQYANPTLQRI*KLYFEDLSYFRKNYSPFCL TCLLLSCGYVKHWVGN*L*NTTKNSCLFSQNNV PVFCSNVISLGSRNALYQSTSKLH*GEVPEPSP FSIFI*FIKKD*SHVDYFTATGVN*PFLVSVFPG ENEAKQEFGFLLMSSYTIHSVNFEKIYPPFSLL GDINYSQEEYNELYSYFDLLKRCYQ*SSCCNIL SFGSVSSDGFSGDLSDKITYKRVPNNYQEASH*L VSFFLFNLLNKSRMLPQKGSVA*VNLFIPSSPI KCRAQDGSSNGLQ*KNLPLRRVSLFR*LGLKA* FTCQGNC*HFPGPP*SC*NLCEPQSREQIELRSP ALGTQTPKGGI*VNFFQVNTCSYV

Frame 2

VIVF*EYFSVVEDLSRSLCLL*LYEIPGGQMFN LFQHICFFSCRNR*LC*DYR*RRYLHHALNQGL *DSKRKNRTWTMYWRRPIWRCTSRHLYESRESS FGGCN*NM*KLYFGQREREISSRSLNNASV*PSS YCEADWSHHRESCLDNHGAVHTWRAEVIFASKE IQFGSSIFDPVCLSA*YSSCISREQKICTQGHC CSECSGVLK*LCKIRRLWIIPIYGR*YLLQSFQR KIAY*MDGSRVNQFSTFYLS**RMDVWCVYVGD TDAWCEAFSRSEEQ*CNRSN*KWGKITNASKLS SYPLQPYDEMLGL*PQQAAQVY*T*SSAQHNPGG REGSARRAHEDGVQKTGHSVLGLRRV**STAQA QQTGLSQSEVQRRILSQPTAHGTNQSLPGFWLP WFTWNHSHGWQHLSRSGISFGPNRFMES*TSGDS NVAAQCGGLYSIGPARDWASVANPSDGRASNPT ATGNGRRSALAGKRGKISET*CETLSRQY*QGG WKSSGSDWKPTYISACG*TRSCSSTKETASPWSS RSSGKPCQPQQPC*QLQRGCQASAPGNQPPSYC QPGPVE**GVRECDGPGESCHRDVQ*NPASPTR GVCPYGEGSRLGPEDIIGHCG*DHSPPTSQHPPR D*DGTEAIEL*PG*AHQQDETGPAVCHDQPPAR VQKANADCCSRPGCGCQKLTRCH*PSKTENAWA DETTLSLP*EHVLLPSFEDVL*PSTSSEELTLCP QSPALTAPTFLNDHLVEKSFSYKFNHTLIWVHF LFCFFQS*YSEKSRIQNVAFF*E*KLYVSF*AS *RTIYVHIKIRSKGGWPRGDILIPKLP*LHSGRG EHEAKNSRKPKRLRILLSTIELLSRLEFLFVRT PFSCNMLIPLYKEYKSYILKT*VISEKTTALFV LPAFYFRVDM*SIGSGTSCRTQLKTHVFFHRIMC QFFVAMLFLLEAEMLCTRAPPNCIEEKFQNHPL FPFLYNL*RKIKAMLTILQPLELTNPSLYLSSQ ERMKQNRNLVFF*CPVTPSILLILKKYTLPLVCW GI*IILRKNIMNCTVTLTY*KGVTSKVLVVISF LLVLFLQMAFQVTCQIR*HTREFPIIIRKPLTN *FLFFYLIF*TSQGCYHRRVAWRKLTFSFHRHLL NAELRMEVAMVFNERTFPCAGSHYSANSDSKHN SLVKEIVSISQAHPSLARISVSHRAGSK*N*DL QH*GLRLQREGFE*IFSR*IHALMC

Sequence PTK2.hAug05

ATTGCGGAGAATATGGCTGACCTAATAGATGGGTACTGCCGGCTGGTGAATGGAACCTCGCAGTCATTTATCATCAGACCTCAGAAAGAAGGTGAACGGG CTTTGCCATCAATACCAAAGTTGGCCAACAGCGAAAAGCAAGGCATGCGGACACACGCCGTCTCTGTGTCAGAAACAGATGATTATGCTGAGATTATAGA TGAAGAAGATACTTACACCATGCCCTCAAAAAGCTATGGAATAGATGAAGCCAGGGATTATGAGATTCAAAGAGAAAGAATAGAACTTGGACGATGTATT GGAGAAGGCCAATTTGGAGATGTACATCAAGGCATTTATATGAGTCCAGAGAATCCAGCTTTGGCGGTTGCAATTAAAACATGTAAAAACTGTACTTCGG ACAGCGTGAGAGAGAAATTTCTTCAAGAAGCCTTAACAATGCGTCAGTTTGACCATCCTCATATTGTGAAGCTGATTGGAGTCATCACAGAGAATCCTGT CTGGATAATCATGGAGCTGTGCACACTTGGAGAGCTGAGGTCATTTTTGCAAGTAAGGAAATACAGTTTGGATCTAGCATCTTTGATCCTGTATGCCTAT CAGCTTAGTACAGCTCTTGCATATCTAGAGAGCAAAAGATTTGTACACAGGGACATTGCTGCTCGGAATGTTCTGGTGTCCTCAAATGATTGTGTAAAAT TAGGAGACTTTGGATTATCCCGATATATGGAAGATAGTACTTACTACAAAGCTTCCAAAGGAAAATTGCCTATTAAATGGATGGCTCCAGAGTCAATCAA TTTTCGACGTTTTACCTCAGCTAGTGACGTATGGATGTTTGGTGTGTGTATGTGGGAGATACTGATGCATGGTGTGAAGCCTTTTCAAGGAGTGAAGAAC AATGATGTAATCGGTCGAATTGAAAATGGGGAAAGATTACCAATGCCTCCAAATTGTCCTCCTACCCTCTACAGCCTTATGACGAAATGCTGGGCCTATG ACCCCAGCAGGCGGCCCAGGTTTACTGAACTTAAAGCTCAGCTCAGCACAATCCTGGAGGAAGAGAAGGCTCAGCAAGAAGAGCGCATGAGGATGGAGTC CAGAAGACAGGCCACAGTGTCCTGGGACTCCGGAGGGTCTGATGAAGCACCGCCCAAGCCCAGCAGACCGGGTTATCCCAGTCCGAGGTCCAGCGAAGGA TTTTATCCCAGCCCACAGCACATGGTACAAACCAATCATTACCAGGTTTCTGGCTACCCTGGTTCACATGGAATCACAGCCATGGCTGGCAGCATCTATC CAGGTCAGGCATCTCTTTTGGACCAAACAGATTCATGGAATCATAGACCTCAGGAGATAGCAATGTGGCAGCCCAATGTGGAGGACTCTACAGTATTGGA CCTGCGAGGGATTGGGCAAGTGTTGCCAACCCATCTGATGGAAGAGCGTCTAATCCGACAGCAACAGGAAATGGAAGAAGATCAGCGCTGGCTGGAAAAA GAGGAAAGATTTCTGAAACCTGATGTGAGACTCTCTCGAGGCAGTATTGACAGGGAGGATGGAAGTCTTCAGGGTCCGATTGGAAACCAACATATATATC AGCCTGTGGGTAAACCAGATCCTGCAGCTCCACCAAAGAAACCGCCTCGCCCTGGAGCTCCCGGTCATCTGGGAAGCCTTGCCAGCCTCAGCAGCCCTGC TGACAGCTACAACGAGGGTGTCAAGCTTCAGCCCCAGGAAATCAGCCCCCCTCCTACTGCCAACCTGGACCGGTCGAATGATAAGGTGTACGAGAATGTG ACGGGCCTGGTGAAAGCTGTCATCGAGATGTCCAGTAAAATCCAGCCAGCCCCACCAGAGGAGTATGTCCCTATGGTGAAGGAAGTCGGCTTGGCCCTGA GGACATTATTGGCCACTGTGGATGAGACCATTCCCCTCCTACCAGCCAGCACCCACCGAGAGATTGAGATGGCACAGAAGCTATTGAACTCTGACCTGGG TGAGCTCATCAACAAGATGAAACTGGCCCAGCAGTATGTCATGACCAGCCTCCAGCAAGAGTACAAAAAGCAAATGCTGACTGCTGCTCACGCCCTGGCT GTGGATGCCAAAAACTTACTCGATGTCATTGACCAAGCAAGACTGAAAATGCTTGGGCAGACGAGACCACACTGAGCCTCCCCTAGGAGCACGTCTTGCT ACCCTCTTTTGAAGATGTTCTCTAGCCTTCCACCAGCAGCGAGGAATTAACCCTGTGTCCTCAGTCGCCAGCACTTACAGCTCCAACTTTTTTGAATGAC CATCTGGTTGAAAAATCTTTCTCATATAAGTTTAACCACACTTTGATTTGGGTTCATTTTTTGTTTTGTTTTTTTCAATCATGATATTCAGAAAAATCCA GGATCCAAAATGTGGCGTTTTTCTAAGAATGAAAATTATATGTAAGCTTTTAAGCATCATGAAGAACAATTTATGTTCACATTAAGATACGTTCTAAAGG GGGATGGCCAAGGGGTGACATCTTAATTCCTAAACTACCTTAGCTGCATAGTGGAAGAGGAGAGCATGAAGCAAAGAATTCCAGGAAACCCAAGAGGCTG AGAATTCTTTTGTCTACCATAGAATTATTATCCAGACTGGAATTTTTGTTTGTTAGAACACCCTTCAGTTGCAATATGCTAATCCCACTTTACAAAGAAT ATAAAAGCTATATTTTGAAGACTTGAGTTATTTCAGAAAAAACTACAGCCCTTTTTGTCTTACCTGCCTTTTACTTTCGTGTGGATATGTGAAGCATTGG GTCGGGAACTAGCTGTAGAACACAACTAAAAACTCATGTCTTTTTTCACAGAATAATGTGCCAGTTTTTTGTAGCAATGTTATTTCTCTTGGAAGCAGAA ATGCTTTGTACCAGAGCACCTCCAAACTGCATTGAGGAGAAGTTCCAGAACCATCCCCTTTTTCCATTTTTATATAATTTATAAAGAAAGATTAAAGCCA TGTTGACTATTTTACAGCCACTGGAGTTAACTAACCCTTCCTTGTATCTGTCTTCCCAGGAGAGAATGAAGCAAAACAGGAATTTGGTTTTCTTTTGATG TCCAGTTACACCATCCATTCTGTTAATTTTGAAAAAATATACCCTCCCTTTAGTTTGTTGGGGGATATAAATTATTCTCAGGAAGAATATAATGAACTGT ACAGTTACTTTGACCTATTAAAAAGGTGTTACCAGTAAAGTTCTTGTTGTAATATCCTTTCTTTTGGTTCTGTTTCTTCAGATGGCTTTTCAGGTGACTT GTCAGATAAGATAACATACAAGAGAGTTCCCAATAATTATCAGGAAGCCTCTCACTAATTAGTTTCTTTTTTTTTATTTAATCTTCTAAACAAGTCAAGG ATGTTACCACAGAAGGGTAGCGTGGCGTAAGTTAACCTTTTCATTCCATCGTCACCTATTAAATGCAGAGCTCAGGATGGAAGTAGCAATGGTCTTCAAT GAAAGAACCTTCCCCTGCGCAGGGTCTCACTATTCCGCTAACTCGGACTCAAAGCATAATTCACTTGTCAAGGAAATTGTTAGCATTTCCCAGGCCCACC CTAGTCTTGCTAGAATCTCTGTGAGCCACAGAGCAGGGAGCAAATAGAACTGAGATCTCCAGCACTAGGGACTCAGACTCCAAAGGGAGGGATTTGAGTG AATTTTTTCCAGGTAAATACATGCTCTTATGTGC

PTK2.au.8.f (forward primer)
PTK2.u.r.53 (reverse primer)
Single exon skipping

Frame 0

IAENMADLIDGYCRLVNGTSQSFIIRPQKEGER ALPSIPKLANSEKQGMRTHAVSVSETDDYAEIID EEDTYTMPSKSYGIDEARDYEIQRERIELGRCI GEGQFGDVHQGIYMSPENPALAVAIKTCKNCTS DSVREKFLQEALTMRQFDHPHIVKLIGVITENPV WIIMELCTLGELRSFLQVRKYSLDLASLILYAY QLSTALAYLESKRFVHRDIAARNVLVSSNDCVK LGDFGLSRYMEDSTYYKASKGKLPIKWMAPESIN FRRFTSASDVWMFGVCMWEILMHGVKPFQGVKN NDVIGRIENGERLPMPPNCPPTLYSLMTKCWAY DPSRRPRFTELKAQLSTILEEEKAQQEERMRMES RRQATVSWDSGGSDEAPPKPSRPGYPSPRSSEG FYPSPQHMVQTNHYQVSGYPGSHGITAMAGSIY PGQASLLDQTDSWNHRPQEIAMWQPNVEDSTVLD LRGIGQVLPTHLMEERLIRQQQEMEEDQRWLEK EERFLKPDVRLSRGSIDREDGSLQGPIGNQHIY QPVGKPDPAAPPKKPPRPGAPGHLGSLASLSSPA DSYNEGVKLQPQEISPPPTANLDRSNDKVYENV TGLVKAVIEMSSKIQPAPPEEYVPMVKEVGLAL RTLLATVDETIPLLPASTHREIEMAQKLLNSDLG ELINKMKLAQQYVMTSLQQEYKKQMLTAAHALA VDAKNLLDVIDQARLKMLGQTRPH*ASPRSTSC YPLLKMFSSLPPAARN*PCVLSRQHLQLQLF*MT IWLKNLSHISLTTL*FGFIFCFVFFNHDIQKNP GSKMWRFSKNENYM*AFKHHEEQFMFTLRYVLK GDGQGVTS*FLNYLSCIVEEESMKQRIPGNPRG* EFFCLP*NYYPDWNFCLLEHPSVAIC*SHFTKN IKAIF*RLELFQKKLQPFLSYLPFTFVWICEAL GRELAVEHN*KLMSFFTE*CASFL*QCYFSWKQK CFVPEHLQTALRRSSRTIPFFHFYIIYKERLKP C*LFYSHWS*LTLPCICLPRRE*SKTGIWFSFD VQLHHPFC*F*KNIPSL*FVGGYKLFSGRI**TV QLL*PIKKVLPVKFLL*YPFFWFCFFRWLFR*L VR*DNIQESSQ*LSGSLSLISFFFFI*SSKQVK DVTTEG*RGVS*PFHSIVTY*MQSSGWK*QWSSM KEPSPAQGLTIPLTRTQSIIHLSRKLLAFPRPT LVLLESL*ATEQGANRTEISSTRDSDSKGRDLS EFFPGKYMLLC

Frame 1

LRRIWLT**MGTAGW*MEPRSHLSSDLRKKVNG LCHQYQSWPTAKSKACGHTPSLCQKQMIMLRL* MKKILTPCPQKAME*MKPGIMRFKEKE*NLDDVL EKANLEMYIKAFI*VQRIQLWRLQLKHVKTVLR TA*ERNFFKKP*QCVSLTILIL*S*LESSQRIL SG*SWSCAHLES*GHFCK*GNTVWI*HL*SCMPI SLVQLLHI*RAKDLYTGTLLLGMFWCPQMIV*N *ETLDYPDIWKIVLTTKLPKENCLLNGWLQSQS IFDVLPQLVTYGCLVCVCGRY*CMV*SLFKE*RT MM*SVELKMGKDYQCLQIVLLPSTAL*RNAGPM TPAGGPGLLNLKLSSAQSWRKRRLSKKSA*GWS PEDRPQCPGTPEGLMKHRPSPADRVIPVRGPAKD FIPAHSTWYKPIITRFLATLVHMESQPWLAASI QVRHLFWTKQIHGIIDLRR*QCGSPMWRTLQYW TCEGLGKCCQPI*WKSV*SDSNRKWKKISAGWKK RKDF*NLM*DSLEAVLTGRMEVFRVRLETNIYI SLWVNQILQLHQRNRLALELPVIWEALPASAAL LTATTRVSSFSPRKSAPLLLPTWTGRMIRCTRM* RAW*KLSSRCPVKSSQPHQRSMSLW*RKSAWP* GHYWPLWMRPFPSYQPAPTERLRWHRSY*TLTW VSSSTR*NWPSSMS*PASSKSTKSKC*LLLTPWL WMPKTYSMSLTKQD*KCLGRRDHTEPPLGARLA TLF*RCSLAFHQQRGINPVSSVASTYSSNFFE* PSG*KIFLI*V*PHFDLGSFFVLFFSIMIFRKIQ DPKCGVFLRMKIICKLLSIMKNNLCSH*DTF*R GMAKG*HLNS*TTLAA*WKRRA*SKEFQETQEA ENSFVYHRIIIQTGIFVC*NTLQLQYANPTLQRI *KLYFEDLSYFRKNYSPFCLTCLLLSCGYVKHW VGN*L*NTTKNSCLFSQNNVPVFCSNVISLGSR NALYQSTSKLH*GEVPEPSPFSIFI*FIKKD*SH VDYFTATGVN*PFLVSVFPGENEAKQEFGFLLM SSYTIHSVNFEKIYPPFSLLGDINYSQEEYNEL YSYFDLLKRCYQ*SSCCNILSFGSVSSDGFSGDL SDKITYKRVPNNYQEASH*LVSFFLFNLLNKSR MLPQKGSVA*VNLFIPSSPIKCRAQDGSSNGLQ *KNLPLRRVSLFR*LGLKA*FTCQGNC*HFPGPP *SC*NLCEPQSREQIELRSPALGTQTPKGGI*V NFFQVNTCSYV

Frame 2

CGEYG*PNRWVLPAGEWNLAVIYHQTSERR*TG FAINTKVGQQRKARHADTRRLCVRNR*LC*DYR *RRYLHHALKKLWNR*SQGL*DSKRKNRTWTMY WRRPIWRCTSRHLYESRESSFGGCN*NM*KLYFG QREREISSRSLNNASV*PSSYCEADWSHHRESC LDNHGAVHTWRAEVIFASKEIQFGSSIFDPVCL SA*YSSCISREQKICTQGHCCSECSGVLK*LCKI RRLWIIPIYGR*YLLQSFQRKIAY*MDGSRVNQ FSTFYLS**RMDVWCVYVGDTDAWCEAFSRSEE Q*CNRSN*KWGKITNASKLSSYPLQPYDEMLGL* PQQAAQVY*T*SSAQHNPGGREGSARRAHEDGV QKTGHSVLGLRRV**STAQAQQTGLSQSEVQRR ILSQPTAHGTNQSLPGFWLPWFTWNHSHGWQHLS RSGISFGPNRFMES*TSGDSNVAAQCGGLYSIG PARDWASVANPSDGRASNPTATGNGRRSALAGK RGKISET*CETLSRQY*QGGWKSSGSDWKPTYIS ACG*TRSCSSTKETASPWSSRSSGKPCQPQQPC *QLQRGCQASAPGNQPPSYCQPGPVE**GVREC DGPGESCHRDVQ*NPASPTRGVCPYGEGSRLGPE DIIGHCG*DHSPPTSQHPPRD*DGTEAIEL*PG *AHQQDETGPAVCHDQPPARVQKANADCCSRPG CGCQKLTRCH*PSKTENAWADETTLSLP*EHVLL PSFEDVL*PSTSSEELTLCPQSPALTAPTFLND HLVEKSFSYKFNHTLIWVHFLFCFFQS*YSEKS RIQNVAFF*E*KLYVSF*AS*RTIYVHIKIRSKG GWPRGDILIPKLP*LHSGRGEHEAKNSRKPKRL RILLSTIELLSRLEFLFVRTPFSCNMLIPLYKE YKSYILKT*VISEKTTALFVLPAFYFRVDM*SIG SGTSCRTQLKTHVFFHRIMCQFFVAMLFLLEAE MLCTRAPPNCIEEKFQNHPLFPFLYNL*RKIKA MLTILQPLELTNPSLYLSSQERMKQNRNLVFF*C PVTPSILLILKKYTLPLVCWGI*IILRKNIMNC TVTLTY*KGVTSKVLVVISFLLVLFLQMAFQVT CQIR*HTREFPIIIRKPLTN*FLFFYLIF*TSQG CYHRRVAWRKLTFSFHRHLLNAELRMEVAMVFN ERTFPCAGSHYSANSDSKHNSLVKEIVSISQAH PSLARISVSHRAGSK*N*DLQH*GLRLQREGFE* IFSR*IHALMC

Sequence PTK2.gAug05

CAGTTTCCCCAGAGCTCCTCAAGAATAACGGAAGGGAGAATATGACAGATACCTAGCATCTAGCAAAATAATGGCAGCTGCTTACCTTGACCCCAACTTG AATCACACACCAAATTCGAGTACTAAGACTCACCTGGGTACTGGTATGGAACGTTCTCCTGGTGCAATGGAGCGAGTATTAAAGGTCTTTCATTATTTTG AAAGCAATAGTGAGCCAACCACCTGGGCCAGTATTATCAGGCATGGAGATGCTACTGATGTCAGGATATGAATTGAGAATTCGTTATTTGCCAAAAGGAT TTCTAAACCAGTTTACTGAAGATAAGCCAACTTTGAATTTCTTCTATCAACAGGTGAAGAGCGATTATATGTTAGAGATAGCTGATCAAGTGGACCAGGA AATTGCTTTGAAGTTGGGTTGTCTAGAAATACGGCGATCATACTGGGAGATGCGGGGCAATGCACTAGAAAAGAAGTCTAACTATGAAGTATTAGAAAAA GATGTTGGTTTAAAGCGATTTTTTCCTAAGAGTTTACTGGATTCTGTCAAGGCCAAAACACTAAGAAAACTGATCCAACAAACATTTAGACAATTTGCCA ACCTTAATAGAGAAGAAAGTATTCTGAAATTCTTTGAGATCCTGTCTCCAGTCTACAGATTTGATAAGGAATGCTTCAAGTGTGCTCTTGGTTCAAGCTG GATTATTTCAGTGGAACTGGCAATCGGCCCAGAAGAAGGAATCAGTTACCTAACGGACAAGGGCTGCAATCCCACACATCTTGCTGACTTCACTCAAGTG CAAACCATTCAGTATTCAAACAGTGAAGACAAGGACAGAAAAGGAATGCTACAACTAAAAATAGCAGGTGCACCCGAGCCTCTGACAGTGACGGCACCAT CCCTAACCATTGCGGAGAATATGGCTGACCTAATAGATGGGTACTGCCGGCTGGTGAATGGAACCTCGCAGTCATTTATCATCAGACCTCAGAAAGAAGG TGAACGGGCTTTGCCATCAATACCAAAGTTGGCCAACAGCGAAAAGCAAGGCATGCGGACACACGCCGTCTCTGTGTCAGAAACAGATGATTATGCTGAG ATTATAGATGAAGAAGATACTTACACCATGCCCTCAACCAGGGATTATGAGATTCAAAGAGAAAGAATAGAACTTGGACGATGTATTGGAGAAGGCCAAT TTGGAGATGTACATCAAGGCATTTATATGAGTCCAGAGAATCCAGCTTTGGCGGTTGCAATTAAAACATGTAAAAACTGTACTTCGGACAGCGTGAGAGA GAAATTTCTTCAAGAAGCCTGCCATTACACATCTTTGCACTGGAATTGGTGCAGATATATAAGTGATCCTAATGTTGATGCTGCCCAGACCCCAGGAATG CAGAGGTAACAATGCGTCAGTTTGACCATCCTCATATTGTGAAGCTGATTGGAGTCATCACAGAGAATCCTGTCTGGATAATCATGGAGCTGTGCACACT TGGAGAGCTGAGGTCATTTTTGCAAGTAAGGAAATACAGTTTGGATCTAGCATCTTTGATCCTGTATGCCTATCAGCTTAGTACAGCTCTTGCATATCTA GAGAGCAAAAGATTTGTACACAGGGACATTGCTGCTCGGAATGTTCTGGTGTCCTCAAATGATTGTGTAAAATTAGGAGACTTTGGATTATCCCGATATA TGGAAGATAGTACTTACTACAAAGCTTCCAAAGGAAAATTGCCTATTAAATGGATGGCTCCAGAGTCAATCAATTTTCGACGTTTTACCTCAGCTAGTGA CGTATGGATGTTTGGTGTGTGTATGTGGGAGATACTGATGCATGGTGTGAAGCCTTTTCAAGGAGTGAAGAACAATGATGTAATCGGTCGAATTGAAAAT GGGGAAAGATTACCAATGCCTCCAAATTGTCCTCCTACCCTCTACAGCCTTATGACGAAATGCTGGGCCTATGACCCCAGCAGGCGGCCCAGGTTTACTG AACTTAAAGCTCAGCTCAGCACAATCCTGGAGGAAGAGAAGGCTCAGCAAGAAGAGCGCATGAGGATGGAGTCCAGAAGACAGGCCACAGTGTCCTGGGA CTCCGGAGGGTCTGATGAAGCACCGCCCAAGCCCAGCAGACCGGGTTATCCCAGTCCGAGGTCCAGCGAAGGATTTTATCCCAGCCCACAGCACATGGTA CAAACCAATCATTACCAGGTTTCTGGCTACCCTGGTTCACATGGAATCACAGCCATGGCTGGCAGCATCTATCCAGGTCAGGCATCTCTTTTGGACCAAA CAGATTCATGGAATCATAGACCTCAGGAGATAGCAATGTGGCAGCCCAATGTGGAGGACTCTACAGTATTGGACCTGCGAGGGATTGGGCAAGTGTTGCC AACCCATCTGATGGAAGAGCGTCTAATCCGACAGCAACAGGAAATGGAAGAAGATCAGCGCTGGCTGGAAAAAGAGGAAAGATTTCTGATTGGAAACCAA CATATATATCAGCCTGTGGGTAAACCAGATCCTGCAGCTCCACCAAAGAAACCGCCTCGCCCTGGAGCTCCCGGTCATCTGGGAAGCCTTGCCAGCCTCA GCAGCCCTGCTGACAGCTACAACGAGGGTGTCAAGCTTCAGCCCCAGGAAATCAGCCCCCCTCCTACTGCCAACCTGGACCGGTCGAATGATAAGGTGTA CGAGAATGTGACGGGCCTGGTGAAAGCTGTCATCGAGATGTCCAGTAAAATCCAGCCAGCCCCACCAGAGGAGTATGTCCCTATGGTGAAGGAAGTCGGC TTGGCCCTGAGGACATTATTGGCCACTGTGGATGAGACCATTCCCCTCCTACCAGCCAGCACCCACCGAGAGATTGAGATGGCACAGAAGCTATTGAACT CTGACCTGGGTGAGCTCATCAACAAGATGAAACTGGCCCAGCAGTATGTCATGACCAGCCTCCAGCAAGAGTACAAAAAGCAAATGCTGACTGCTGCTCA CGCCCTGGCTGTGGATGCCAAAAACTTACTCGATGTCATTGACCAAGCAAGACTGAAAATGCTTGGGCAGACGAGACCACACTGAGCCTCCCCTAGGAGC ACGTCTTGCTACCCTCTTTTGAAGATGTTCTCTAGCCTTCCACCAGCAGCGAGGAATTAACCCTGTGTCCTCAGTCGCCAGCACTTACAGCTCCAACTTT TTTGAATGACCATCTGGTTGAAAAATCTTTCTCATATAAGTTTAACCACACTTTGATTTGGGTTCATTTTTTGTTTTGTTTTTTTCAATCATGATATTCA GAAAAATCCAGGATCCAAAATGTGGCGTTTTTCTAAGAATGAAAATTATATGTAAGCTTTTAAGCATCATGAAGAACAATTTATGTTCACATTAAGATAC GTTCTAAAGGGGGATGGCCAAGGGGTGACATCTTAATTCCTAAACTACCTTAGCTGCATAGTGGAAGAGGAGAGCATGAAGCAAAGAATTCCAGGAAACC CAAGAGGCTGAGAATTCTTTTGTCTACCATAGAATTATTATCCAGACTGGAATTTTTGTTTGTTAGAACACCCTTCAGTTGCAATATGCTAATCCCACTT TACAAAGAATATAAAAGCTATATTTTGAAGACTTGAGTTATTTCAGAAAAAACTACAGCCCTTTTTGTCTTACCTGCCTTTTACTTTCGTGTGGATATGT GAAGCATTGGGTCGGGAACTAGCTGTAGAACACAACTAAAAACTCATGTCTTTTTTCACAGAATAATGTGCCAGTTTTTTGTAGCAATGTTATTTCTCTT GGAAGCAGAAATGCTTTGTACCAGAGCACCTCCAAACTGCATTGAGGAGAAGTTCCAGAACCATCCCCTTTTTCCATTTTTATATAATTTATAAAGAAAG ATTAAAGCCATGTTGACTATTTTACAGCCACTGGAGTTAACTAACCCTTCCTTGTATCTGTCTTCCCAGGAGAGAATGAAGCAAAACAGGAATTTGGTTT TCTTTTGATGTCCAGTTACACCATCCATTCTGTTAATTTTGAAAAAATATACCCTCCCTTTAGTTTGTTGGGGGATATAAATTATTCTCAGGAAGAATAT AATGAACTGTACAGTTACTTTGACCTATTAAAAAGGTGTTACCAGTAAAGTTCTTGTTGTAATATCCTTTCTTTTGGTTCTGTTTCTTCAGATGGCTTTT CAGGTGACTTGTCAGATAAGATAACATACAAGAGAGTTCCCAATAATTATCAGGAAGCCTCTCACTAATTAGTTTCTTTTTTTTTATTTAATCTTCTAAA CAAGTCAAGGATGTTACCACAGAAGGGTAGCGTGGCGTAAGTTAACCTTTTCATTCCATCGTCACCTATTAAATGCAGAGCTCAGGATGGAAGTAGCAAT GGTCTTCAATGAAAGAACCTTCCCCTGCGCAGGGTCTCACTATTCCGCTAACTCGGACTCAAAGCATAATTCACTTGTCAAGGAAATTGTTAGCATTTCC CAGGCCCACCCTAGTCTTGCTAGAATCTCTGTGAGCCACAGAGCAGGGAGCAAATAGAACTGAGATCTCCAGCACTAGGGACTCAGACTCCAAAGGGAGG GATTTGAGTGAATTTTTTCCAGGTAAATACATGCTCTTATGTGC

PTK2.au.8.f (forward primer)
PTK2.u.r.53 (reverse primer)
Single exon skipping

Frame 0

QFPQSSSRITEGRI*QIPSI*QNNGSCLP*PQL ESHTKFEY*DSPGYWYGTFSWCNGASIKGLSLF* KQ**ANHLGQYYQAWRCY*CQDMN*EFVICQKD F*TSLLKISQL*ISSINR*RAIIC*R*LIKWTR KLL*SWVV*KYGDHTGRCGAMH*KRSLTMKY*KK MLV*SDFFLRVYWILSRPKH*EN*SNKHLDNLP TLIEKKVF*NSLRSCLQSTDLIRNASSVLLVQA GLFQWNWQSAQKKESVT*RTRAAIPHILLTSLKC KPFSIQTVKTRTEKECYN*K*QVHPSL*Q*RHH P*PLRRIWLT**MGTAGW*MEPRSHLSSDLRKK VNGLCHQYQSWPTAKSKACGHTPSLCQKQMIMLR L*MKKILTPCPQPGIMRFKEKE*NLDDVLEKAN LEMYIKAFI*VQRIQLWRLQLKHVKTVLRTA*E RNFFKKPAITHLCTGIGADI*VILMLMLPRPQEC RGNNASV*PSSYCEADWSHHRESCLDNHGAVHT WRAEVIFASKEIQFGSSIFDPVCLSA*YSSCIS REQKICTQGHCCSECSGVLK*LCKIRRLWIIPIY GR*YLLQSFQRKIAY*MDGSRVNQFSTFYLS** RMDVWCVYVGDTDAWCEAFSRSEEQ*CNRSN*K WGKITNASKLSSYPLQPYDEMLGL*PQQAAQVY* T*SSAQHNPGGREGSARRAHEDGVQKTGHSVLG LRRV**STAQAQQTGLSQSEVQRRILSQPTAHG TNQSLPGFWLPWFTWNHSHGWQHLSRSGISFGPN RFMES*TSGDSNVAAQCGGLYSIGPARDWASVA NPSDGRASNPTATGNGRRSALAGKRGKISDWKP TYISACG*TRSCSSTKETASPWSSRSSGKPCQPQ QPC*QLQRGCQASAPGNQPPSYCQPGPVE**GV RECDGPGESCHRDVQ*NPASPTRGVCPYGEGSR LGPEDIIGHCG*DHSPPTSQHPPRD*DGTEAIEL *PG*AHQQDETGPAVCHDQPPARVQKANADCCS RPGCGCQKLTRCH*PSKTENAWADETTLSLP*E HVLLPSFEDVL*PSTSSEELTLCPQSPALTAPTF LNDHLVEKSFSYKFNHTLIWVHFLFCFFQS*YS EKSRIQNVAFF*E*KLYVSF*AS*RTIYVHIKI RSKGGWPRGDILIPKLP*LHSGRGEHEAKNSRKP KRLRILLSTIELLSRLEFLFVRTPFSCNMLIPL YKEYKSYILKT*VISEKTTALFVLPAFYFRVDM *SIGSGTSCRTQLKTHVFFHRIMCQFFVAMLFLL EAEMLCTRAPPNCIEEKFQNHPLFPFLYNL*RK IKAMLTILQPLELTNPSLYLSSQERMKQNRNLV FF*CPVTPSILLILKKYTLPLVCWGI*IILRKNI MNCTVTLTY*KGVTSKVLVVISFLLVLFLQMAF QVTCQIR*HTREFPIIIRKPLTN*FLFFYLIF* TSQGCYHRRVAWRKLTFSFHRHLLNAELRMEVAM VFNERTFPCAGSHYSANSDSKHNSLVKEIVSIS QAHPSLARISVSHRAGSK*N*DLQH*GLRLQRE GFE*IFSR*IHALMC

Frame 1

SFPRAPQE*RKGEYDRYLASSKIMAAAYLDPNL NHTPNSSTKTHLGTGMERSPGAMERVLKVFHYF ESNSEPTTWASIIRHGDATDVRI*IENSLFAKRI SKPVY*R*ANFEFLLSTGEERLYVRDS*SSGPG NCFEVGLSRNTAIILGDAGQCTRKEV*L*SIRK RCWFKAIFS*EFTGFCQGQNTKKTDPTNI*TICQ P**RRKYSEIL*DPVSSLQI**GMLQVCSWFKL DYFSGTGNRPRRRNQLPNGQGLQSHTSC*LHSS ANHSVFKQ*RQGQKRNATTKNSRCTRASDSDGTI PNHCGEYG*PNRWVLPAGEWNLAVIYHQTSERR *TGFAINTKVGQQRKARHADTRRLCVRNR*LC* DYR*RRYLHHALNQGL*DSKRKNRTWTMYWRRPI WRCTSRHLYESRESSFGGCN*NM*KLYFGQRER EISSRSLPLHIFALELVQIYK*S*C*CCPDPRN AEVTMRQFDHPHIVKLIGVITENPVWIIMELCTL GELRSFLQVRKYSLDLASLILYAYQLSTALAYL ESKRFVHRDIAARNVLVSSNDCVKLGDFGLSRY MEDSTYYKASKGKLPIKWMAPESINFRRFTSASD VWMFGVCMWEILMHGVKPFQGVKNNDVIGRIEN GERLPMPPNCPPTLYSLMTKCWAYDPSRRPRFT ELKAQLSTILEEEKAQQEERMRMESRRQATVSWD SGGSDEAPPKPSRPGYPSPRSSEGFYPSPQHMV QTNHYQVSGYPGSHGITAMAGSIYPGQASLLDQ TDSWNHRPQEIAMWQPNVEDSTVLDLRGIGQVLP THLMEERLIRQQQEMEEDQRWLEKEERFLIGNQ HIYQPVGKPDPAAPPKKPPRPGAPGHLGSLASL SSPADSYNEGVKLQPQEISPPPTANLDRSNDKVY ENVTGLVKAVIEMSSKIQPAPPEEYVPMVKEVG LALRTLLATVDETIPLLPASTHREIEMAQKLLN SDLGELINKMKLAQQYVMTSLQQEYKKQMLTAAH ALAVDAKNLLDVIDQARLKMLGQTRPH*ASPRS TSCYPLLKMFSSLPPAARN*PCVLSRQHLQLQL F*MTIWLKNLSHISLTTL*FGFIFCFVFFNHDIQ KNPGSKMWRFSKNENYM*AFKHHEEQFMFTLRY VLKGDGQGVTS*FLNYLSCIVEEESMKQRIPGN PRG*EFFCLP*NYYPDWNFCLLEHPSVAIC*SHF TKNIKAIF*RLELFQKKLQPFLSYLPFTFVWIC EALGRELAVEHN*KLMSFFTE*CASFL*QCYFS WKQKCFVPEHLQTALRRSSRTIPFFHFYIIYKER LKPC*LFYSHWS*LTLPCICLPRRE*SKTGIWF SFDVQLHHPFC*F*KNIPSL*FVGGYKLFSGRI **TVQLL*PIKKVLPVKFLL*YPFFWFCFFRWLF R*LVR*DNIQESSQ*LSGSLSLISFFFFI*SSK QVKDVTTEG*RGVS*PFHSIVTY*MQSSGWK*Q WSSMKEPSPAQGLTIPLTRTQSIIHLSRKLLAFP RPTLVLLESL*ATEQGANRTEISSTRDSDSKGR DLSEFFPGKYMLLC

Frame 2

VSPELLKNNGRENMTDT*HLAK*WQLLTLTPT* ITHQIRVLRLTWVLVWNVLLVQWSEY*RSFIIL KAIVSQPPGPVLSGMEMLLMSGYELRIRYLPKG FLNQFTEDKPTLNFFYQQVKSDYMLEIADQVDQE IALKLGCLEIRRSYWEMRGNALEKKSNYEVLEK DVGLKRFFPKSLLDSVKAKTLRKLIQQTFRQFA NLNREESILKFFEILSPVYRFDKECFKCALGSSW IISVELAIGPEEGISYLTDKGCNPTHLADFTQV QTIQYSNSEDKDRKGMLQLKIAGAPEPLTVTAP SLTIAENMADLIDGYCRLVNGTSQSFIIRPQKEG ERALPSIPKLANSEKQGMRTHAVSVSETDDYAE IIDEEDTYTMPSTRDYEIQRERIELGRCIGEGQ FGDVHQGIYMSPENPALAVAIKTCKNCTSDSVRE KFLQEACHYTSLHWNWCRYISDPNVDAAQTPGM QR*QCVSLTILIL*S*LESSQRILSG*SWSCAH LES*GHFCK*GNTVWI*HL*SCMPISLVQLLHI* RAKDLYTGTLLLGMFWCPQMIV*N*ETLDYPDI WKIVLTTKLPKENCLLNGWLQSQSIFDVLPQLV TYGCLVCVCGRY*CMV*SLFKE*RTMM*SVELKM GKDYQCLQIVLLPSTAL*RNAGPMTPAGGPGLL NLKLSSAQSWRKRRLSKKSA*GWSPEDRPQCPG TPEGLMKHRPSPADRVIPVRGPAKDFIPAHSTWY KPIITRFLATLVHMESQPWLAASIQVRHLFWTK QIHGIIDLRR*QCGSPMWRTLQYWTCEGLGKCC QPI*WKSV*SDSNRKWKKISAGWKKRKDF*LETN IYISLWVNQILQLHQRNRLALELPVIWEALPAS AALLTATTRVSSFSPRKSAPLLLPTWTGRMIRC TRM*RAW*KLSSRCPVKSSQPHQRSMSLW*RKSA WP*GHYWPLWMRPFPSYQPAPTERLRWHRSY*T LTWVSSSTR*NWPSSMS*PASSKSTKSKC*LLL TPWLWMPKTYSMSLTKQD*KCLGRRDHTEPPLGA RLATLF*RCSLAFHQQRGINPVSSVASTYSSNF FE*PSG*KIFLI*V*PHFDLGSFFVLFFSIMIF RKIQDPKCGVFLRMKIICKLLSIMKNNLCSH*DT F*RGMAKG*HLNS*TTLAA*WKRRA*SKEFQET QEAENSFVYHRIIIQTGIFVC*NTLQLQYANPT LQRI*KLYFEDLSYFRKNYSPFCLTCLLLSCGYV KHWVGN*L*NTTKNSCLFSQNNVPVFCSNVISL GSRNALYQSTSKLH*GEVPEPSPFSIFI*FIKK D*SHVDYFTATGVN*PFLVSVFPGENEAKQEFGF LLMSSYTIHSVNFEKIYPPFSLLGDINYSQEEY NELYSYFDLLKRCYQ*SSCCNILSFGSVSSDGF SGDLSDKITYKRVPNNYQEASH*LVSFFLFNLLN KSRMLPQKGSVA*VNLFIPSSPIKCRAQDGSSN GLQ*KNLPLRRVSLFR*LGLKA*FTCQGNC*HF PGPP*SC*NLCEPQSREQIELRSPALGTQTPKGG I*VNFFQVNTCSYV

Sequence PTK2.fAug05

TCCCGGACCACTGTGAGCCCGCGGCGTGAGGCGTGGGAGGAAGCGCGGCTGCTGTCGCCCAGCGCCGCCCCGTCGTCGTCTGCCTTCGCTTCACGGCGCC GAGCCGCGGTCCGAAATATGACAGATACCTAGCATCTAGCAAAATAATGGCAGCTGCTTACCTTGACCCCAACTTGAATCACACACCAAATTCGAGTACT AAGACTCACCTGGGTACTGGTATGGAACGTTCTCCTGGTGCAATGGAGCGAGTATTAAAGGTCTTTCATTATTTTGAAAGCAATAGTGAGCCAACCACCT GGGCCAGTATTATCAGGCATGGAGATGCTACTGATGTCAGGATATGAATTGAGAATTCGTTATTTGCCAAAAGGATTTCTAAACCAGTTTACTGAAGATA AGCCAACTTTGAATTTCTTCTATCAACAGGTGAAGAGCGATTATATGTTAGAGATAGCTGATCAAGTGGACCAGGAAATTGCTTTGAAGTTGGGTTGTCT AGAAATACGGCGATCATACTGGGAGATGCGGGGCAATGCACTAGAAAAGAAGTCTAACTATGAAGTATTAGAAAAAGATGTTGGTTTAAAGCGATTTTTT CCTAAGAGTTTACTGGATTCTGTCAAGGCCAAAACACTAAGAAAACTGATCCAACAAACATTTAGACAATTTGCCAACCTTAATAGAGAAGAAAGTATTC TGAAATTCTTTGAGATCCTGTCTCCAGTCTACAGATTTGATAAGGAATGCTTCAAGTGTGCTCTTGGTTCAAGCTGGATTATTTCAGTGGAACTGGCAAT CGGCCCAGAAGAAGGAATCAGTTACCTAACGGACAAGGGCTGCAATCCCACACATCTTGCTGACTTCACTCAAGTGCAAACCATTCAGTATTCAAACAGT GAAGACAAGGACAGAAAAGGAATGCTACAACTAAAAATAGCAGGTGCACCCGAGCCTCTGACAGTGACGGCACCATCCCTAACCATTGCGGAGAATATGG CTGACCTAATAGATGGGTACTGCCGGCTGGTGAATGGAACCTCGCAGTCATTTATCATCAGACCTCAGAAAGAAGGTGAACGGGCTTTGCCATCAATACC AAAGTTGGCCAACAGCGAAAAGCAAGGCATGCGGACACACGCCGTCTCTGTGTCAGATGAAATTAGTGGGGACGAAACAGATGATTATGCTGAGATTATA GATGAAGAAGATACTTACACCATGCCCTCAAAAAGCTATGGAATAGATGAAGCCAGGGATTATGAGATTCAAAGAGAAAGAATAGAACTTGGACGATGTA TTGGAGAAGGCCAATTTGGAGATGTACATCAAGGCATTTATATGAGTCCAGAGAATCCAGCTTTGGCGGTTGCAATTAAAACATGTAAAAACTGTACTTC GGACAGCGTGAGAGAGAAATTTCTTCAAGAAGCCTTAACAATGCGTCAGTTTGACCATCCTCATATTGTGAAGCTGATTGGAGTCATCACAGAGAATCCT GTCTGGATAATCATGGAGCTGTGCACACTTGGAGAGCTGAGGTCATTTTTGCAAGTAAGGAAATACAGTTTGGATCTAGCATCTTTGATCCTGTATGCCT ATCAGCTTAGTACAGCTCTTGCATATCTAGAGAGCAAAAGATTTGTACACAGGGACATTGCTGCTCGGAATGTTCTGGTGTCCTCAAATGATTGTGTAAA ATTAGGAGACTTTGGATTATCCCGATATATGGAAGATAGTACTTACTACAAAGCTTCCAAAGGAAAATTGCCTATTAAATGGATGGCTCCAGAGTCAATC AATTTTCGACGTTTTACCTCAGCTAGTGACGTATGGATGTTTGGTGTGTGTATGTGGGAGATACTGATGCATGGTGTGAAGCCTTTTCAAGGAGTGAAGA ACAATGATGTAATCGGTCGAATTGAAAATGGGGAAAGATTACCAATGCCTCCAAATTGTCCTCCTACCCTCTACAGCCTTATGACGAAATGCTGGGCCTA TGACCCCAGCAGGCGGCCCAGGTTTACTGAACTTAAAGCTCAGCTCAGCACAATCCTGGAGGAAGAGAAGGCTCAGCAAGAAGAGCGCATGAGGATGGAG TCCAGAAGACAGGCCACAGTGTCCTGGGACTCCGGAGGGTCTGATGAAGCACCGCCCAAGCCCAGCAGACCGGGTTATCCCAGTCCGAGGTCCAGCGAAG GATTTTATCCCAGCCCACAGCACATGGTACAAACCAATCATTACCAGGTTTCTGGCTACCCTGGTTCACATGGAATCACAGCCATGGCTGGCAGCATCTA TCCAGGTCAGGCATCTCTTTTGGACCAAACAGATTCATGGAATCATAGACCTCAGGAGATAGCAATGTGGCAGCCCAATGTGGAGGACTCTACAGTATTG GACCTGCGAGGGATTGGGCAAGTGTTGCCAACCCATCTGATGGAAGAGCGTCTAATCCGACAGCAACAGGAAATGGAAGAAGATCAGCGCTGGCTGGAAA AAGAGGAAAGATTTCTGAAACCTGATGTGAGACTCTCTCGAGGCAGTATTGACAGGGAGGATGGAAGTCTTCAGGGTCCGATTGGAAACCAACATATATA TCAGCCTGTGGGTAAACCAGATCCTGCAGCTCCACCAAAGAAACCGCCTCGCCCTGGAGCTCCCGGTCATCTGGGAAGCCTTGCCAGCCTCAGCAGCCCT GCTGACAGCTACAACGAGGGTGTCAAGCTTCAGCCCCAGGAAATCAGCCCCCCTCCTACTGCCAACCTGGACCGGTCGAATGATAAGGTGTACGAGAATG TGACGGGCCTGGTGAAAGCTGTCATCGAGATGTCCAGTAAAATCCAGCCAGCCCCACCAGAGGAGTATGTCCCTATGGTGAAGGAAGTCGGCTTGGCCCT GAGGACATTATTGGCCACTGTGGATGAGACCATTCCCCTCCTACCAGCCAGCACCCACCGAGAGATTGAGATGGCACAGAAGCTATTGAACTCTGACCTG GGTGAGCTCATCAACAAGATGAAACTGGCCCAGCAGTATGTCATGACCAGCCTCCAGCAAGAGTACAAAAAGCAAATGCTGACTGCTGCTCACGCCCTGG CTGTGGATGCCAAAAACTTACTCGATGTCATTGACCAAGCAAGACTGAAAATGCTTGGGCAGACGAGACCACACTGAGCCTCCCCTAGGAGCACGTCTTG CTACCCTCTTTTGAAGATGTTCTCTAGCCTTCCACCAGCAGCGAGGAATTAACCCTGTGTCCTCAGTCGCCAGCACTTACAGCTCCAACTTTTTTGAATG ACCATCTGGTTGAAAAATCTTTCTCATATAAGTTTAACCACACTTTGATTTGGGTTCATTTTTTGTTTTGTTTTTTTCAATCATGATATTCAGAAAAATC CAGGATCCAAAATGTGGCGTTTTTCTAAGAATGAAAATTATATGTAAGCTTTTAAGCATCATGAAGAACAATTTATGTTCACATTAAGATACGTTCTAAA GGGGGATGGCCAAGGGGTGACATCTTAATTCCTAAACTACCTTAGCTGCATAGTGGAAGAGGAGAGCATGAAGCAAAGAATTCCAGGAAACCCAAGAGGC TGAGAATTCTTTTGTCTACCATAGAATTATTATCCAGACTGGAATTTTTGTTTGTTAGAACACCCTTCAGTTGCAATATGCTAATCCCACTTTACAAAGA ATATAAAAGCTATATTTTGAAGACTTGAGTTATTTCAGAAAAAACTACAGCCCTTTTTGTCTTACCTGCCTTTTACTTTCGTGTGGATATGTGAAGCATT GGGTCGGGAACTAGCTGTAGAACACAACTAAAAACTCATGTCTTTTTTCACAGAATAATGTGCCAGTTTTTTGTAGCAATGTTATTTCTCTTGGAAGCAG AAATGCTTTGTACCAGAGCACCTCCAAACTGCATTGAGGAGAAGTTCCAGAACCATCCCCTTTTTCCATTTTTATATAATTTATAAAGAAAGATTAAAGC CATGTTGACTATTTTACAGCCACTGGAGTTAACTAACCCTTCCTTGTATCTGTCTTCCCAGGAGAGAATGAAGCAAAACAGGAATTTGGTTTTCTTTTGA TGTCCAGTTACACCATCCATTCTGTTAATTTTGAAAAAATATACCCTCCCTTTAGTTTGTTGGGGGATATAAATTATTCTCAGGAAGAATATAATGAACT GTACAGTTACTTTGACCTATTAAAAAGGTGTTACCAGTAAAGTTCTTGTTGTAATATCCTTTCTTTTGGTTCTGTTTCTTCAGATGGCTTTTCAGGTGAC TTGTCAGATAAGATAACATACAAGAGAGTTCCCAATAATTATCAGGAAGCCTCTCACTAATTAGTTTCTTTTTTTTTATTTAATCTTCTAAACAAGTCAA GGATGTTACCACAGAAGGGTAGCGTGGCGTAAGTTAACCTTTTCATTCCATCGTCACCTATTAAATGCAGAGCTCAGGATGGAAGTAGCAATGGTCTTCA ATGAAAGAACCTTCCCCTGCGCAGGGTCTCACTATTCCGCTAACTCGGACTCAAAGCATAATTCACTTGTCAAGGAAATTGTTAGCATTTCCCAGGCCCA CCCTAGTCTTGCTAGAATCTCTGTGAGCCACAGAGCAGGGAGCAAATAGAACTGAGATCTCCAGCACTAGGGACTCAGACTCCAAAGGGAGGGATTTGAG TGAATTTTTTCCAGGTAAATACATGCTCTTATGTGC

PTK2.au.8.f (forward primer)
PTK2.u.r.53 (reverse primer)
Single exon skipping

Frame 0

SRTTVSPRREAWEEARLLSPSAAPSSSAFASRR RAAVRNMTDT*HLAK*WQLLTLTPT*ITHQIRVL RLTWVLVWNVLLVQWSEY*RSFIILKAIVSQPP GPVLSGMEMLLMSGYELRIRYLPKGFLNQFTED KPTLNFFYQQVKSDYMLEIADQVDQEIALKLGCL EIRRSYWEMRGNALEKKSNYEVLEKDVGLKRFF PKSLLDSVKAKTLRKLIQQTFRQFANLNREESI LKFFEILSPVYRFDKECFKCALGSSWIISVELAI GPEEGISYLTDKGCNPTHLADFTQVQTIQYSNS EDKDRKGMLQLKIAGAPEPLTVTAPSLTIAENM ADLIDGYCRLVNGTSQSFIIRPQKEGERALPSIP KLANSEKQGMRTHAVSVSDEISGDETDDYAEII DEEDTYTMPSKSYGIDEARDYEIQRERIELGRC IGEGQFGDVHQGIYMSPENPALAVAIKTCKNCTS DSVREKFLQEALTMRQFDHPHIVKLIGVITENP VWIIMELCTLGELRSFLQVRKYSLDLASLILYA YQLSTALAYLESKRFVHRDIAARNVLVSSNDCVK LGDFGLSRYMEDSTYYKASKGKLPIKWMAPESI NFRRFTSASDVWMFGVCMWEILMHGVKPFQGVK NNDVIGRIENGERLPMPPNCPPTLYSLMTKCWAY DPSRRPRFTELKAQLSTILEEEKAQQEERMRME SRRQATVSWDSGGSDEAPPKPSRPGYPSPRSSE GFYPSPQHMVQTNHYQVSGYPGSHGITAMAGSIY PGQASLLDQTDSWNHRPQEIAMWQPNVEDSTVL DLRGIGQVLPTHLMEERLIRQQQEMEEDQRWLE KEERFLKPDVRLSRGSIDREDGSLQGPIGNQHIY QPVGKPDPAAPPKKPPRPGAPGHLGSLASLSSP ADSYNEGVKLQPQEISPPPTANLDRSNDKVYEN VTGLVKAVIEMSSKIQPAPPEEYVPMVKEVGLAL RTLLATVDETIPLLPASTHREIEMAQKLLNSDL GELINKMKLAQQYVMTSLQQEYKKQMLTAAHAL AVDAKNLLDVIDQARLKMLGQTRPH*ASPRSTSC YPLLKMFSSLPPAARN*PCVLSRQHLQLQLF*M TIWLKNLSHISLTTL*FGFIFCFVFFNHDIQKN PGSKMWRFSKNENYM*AFKHHEEQFMFTLRYVLK GDGQGVTS*FLNYLSCIVEEESMKQRIPGNPRG *EFFCLP*NYYPDWNFCLLEHPSVAIC*SHFTK NIKAIF*RLELFQKKLQPFLSYLPFTFVWICEAL GRELAVEHN*KLMSFFTE*CASFL*QCYFSWKQ KCFVPEHLQTALRRSSRTIPFFHFYIIYKERLK PC*LFYSHWS*LTLPCICLPRRE*SKTGIWFSFD VQLHHPFC*F*KNIPSL*FVGGYKLFSGRI**T VQLL*PIKKVLPVKFLL*YPFFWFCFFRWLFR* LVR*DNIQESSQ*LSGSLSLISFFFFI*SSKQVK DVTTEG*RGVS*PFHSIVTY*MQSSGWK*QWSS MKEPSPAQGLTIPLTRTQSIIHLSRKLLAFPRP TLVLLESL*ATEQGANRTEISSTRDSDSKGRDLS EFFPGKYMLLC

Frame 1

PGPL*ARGVRRGRKRGCCRPAPPRRRLPSLHGA EPRSEI*QIPSI*QNNGSCLP*PQLESHTKFEY *DSPGYWYGTFSWCNGASIKGLSLF*KQ**ANHL GQYYQAWRCY*CQDMN*EFVICQKDF*TSLLKI SQL*ISSINR*RAIIC*R*LIKWTRKLL*SWVV *KYGDHTGRCGAMH*KRSLTMKY*KKMLV*SDFF LRVYWILSRPKH*EN*SNKHLDNLPTLIEKKVF *NSLRSCLQSTDLIRNASSVLLVQAGLFQWNWQ SAQKKESVT*RTRAAIPHILLTSLKCKPFSIQTV KTRTEKECYN*K*QVHPSL*Q*RHHP*PLRRIW LT**MGTAGW*MEPRSHLSSDLRKKVNGLCHQY QSWPTAKSKACGHTPSLCQMKLVGTKQMIMLRL* MKKILTPCPQKAME*MKPGIMRFKEKE*NLDDV LEKANLEMYIKAFI*VQRIQLWRLQLKHVKTVL RTA*ERNFFKKP*QCVSLTILIL*S*LESSQRIL SG*SWSCAHLES*GHFCK*GNTVWI*HL*SCMP ISLVQLLHI*RAKDLYTGTLLLGMFWCPQMIV* N*ETLDYPDIWKIVLTTKLPKENCLLNGWLQSQS IFDVLPQLVTYGCLVCVCGRY*CMV*SLFKE*R TMM*SVELKMGKDYQCLQIVLLPSTAL*RNAGP MTPAGGPGLLNLKLSSAQSWRKRRLSKKSA*GWS PEDRPQCPGTPEGLMKHRPSPADRVIPVRGPAK DFIPAHSTWYKPIITRFLATLVHMESQPWLAAS IQVRHLFWTKQIHGIIDLRR*QCGSPMWRTLQYW TCEGLGKCCQPI*WKSV*SDSNRKWKKISAGWK KRKDF*NLM*DSLEAVLTGRMEVFRVRLETNIY ISLWVNQILQLHQRNRLALELPVIWEALPASAAL LTATTRVSSFSPRKSAPLLLPTWTGRMIRCTRM *RAW*KLSSRCPVKSSQPHQRSMSLW*RKSAWP *GHYWPLWMRPFPSYQPAPTERLRWHRSY*TLTW VSSSTR*NWPSSMS*PASSKSTKSKC*LLLTPW LWMPKTYSMSLTKQD*KCLGRRDHTEPPLGARL ATLF*RCSLAFHQQRGINPVSSVASTYSSNFFE* PSG*KIFLI*V*PHFDLGSFFVLFFSIMIFRKI QDPKCGVFLRMKIICKLLSIMKNNLCSH*DTF* RGMAKG*HLNS*TTLAA*WKRRA*SKEFQETQEA ENSFVYHRIIIQTGIFVC*NTLQLQYANPTLQR I*KLYFEDLSYFRKNYSPFCLTCLLLSCGYVKH WVGN*L*NTTKNSCLFSQNNVPVFCSNVISLGSR NALYQSTSKLH*GEVPEPSPFSIFI*FIKKD*S HVDYFTATGVN*PFLVSVFPGENEAKQEFGFLL MSSYTIHSVNFEKIYPPFSLLGDINYSQEEYNEL YSYFDLLKRCYQ*SSCCNILSFGSVSSDGFSGD LSDKITYKRVPNNYQEASH*LVSFFLFNLLNKS RMLPQKGSVA*VNLFIPSSPIKCRAQDGSSNGLQ *KNLPLRRVSLFR*LGLKA*FTCQGNC*HFPGP P*SC*NLCEPQSREQIELRSPALGTQTPKGGI* VNFFQVNTCSYV

Frame 2

PDHCEPAA*GVGGSAAAVAQRRPVVVCLRFTAP SRGPKYDRYLASSKIMAAAYLDPNLNHTPNSST KTHLGTGMERSPGAMERVLKVFHYFESNSEPTT WASIIRHGDATDVRI*IENSLFAKRISKPVY*R* ANFEFLLSTGEERLYVRDS*SSGPGNCFEVGLS RNTAIILGDAGQCTRKEV*L*SIRKRCWFKAIF S*EFTGFCQGQNTKKTDPTNI*TICQP**RRKYS EIL*DPVSSLQI**GMLQVCSWFKLDYFSGTGN RPRRRNQLPNGQGLQSHTSC*LHSSANHSVFKQ *RQGQKRNATTKNSRCTRASDSDGTIPNHCGEYG *PNRWVLPAGEWNLAVIYHQTSERR*TGFAINT KVGQQRKARHADTRRLCVR*N*WGRNR*LC*DY R*RRYLHHALKKLWNR*SQGL*DSKRKNRTWTMY WRRPIWRCTSRHLYESRESSFGGCN*NM*KLYF GQREREISSRSLNNASV*PSSYCEADWSHHRES CLDNHGAVHTWRAEVIFASKEIQFGSSIFDPVCL SA*YSSCISREQKICTQGHCCSECSGVLK*LCK IRRLWIIPIYGR*YLLQSFQRKIAY*MDGSRVN QFSTFYLS**RMDVWCVYVGDTDAWCEAFSRSEE Q*CNRSN*KWGKITNASKLSSYPLQPYDEMLGL *PQQAAQVY*T*SSAQHNPGGREGSARRAHEDG VQKTGHSVLGLRRV**STAQAQQTGLSQSEVQRR ILSQPTAHGTNQSLPGFWLPWFTWNHSHGWQHL SRSGISFGPNRFMES*TSGDSNVAAQCGGLYSI GPARDWASVANPSDGRASNPTATGNGRRSALAGK RGKISET*CETLSRQY*QGGWKSSGSDWKPTYI SACG*TRSCSSTKETASPWSSRSSGKPCQPQQP C*QLQRGCQASAPGNQPPSYCQPGPVE**GVREC DGPGESCHRDVQ*NPASPTRGVCPYGEGSRLGP EDIIGHCG*DHSPPTSQHPPRD*DGTEAIEL*P G*AHQQDETGPAVCHDQPPARVQKANADCCSRPG CGCQKLTRCH*PSKTENAWADETTLSLP*EHVL LPSFEDVL*PSTSSEELTLCPQSPALTAPTFLN DHLVEKSFSYKFNHTLIWVHFLFCFFQS*YSEKS RIQNVAFF*E*KLYVSF*AS*RTIYVHIKIRSK GGWPRGDILIPKLP*LHSGRGEHEAKNSRKPKR LRILLSTIELLSRLEFLFVRTPFSCNMLIPLYKE YKSYILKT*VISEKTTALFVLPAFYFRVDM*SI GSGTSCRTQLKTHVFFHRIMCQFFVAMLFLLEA EMLCTRAPPNCIEEKFQNHPLFPFLYNL*RKIKA MLTILQPLELTNPSLYLSSQERMKQNRNLVFF* CPVTPSILLILKKYTLPLVCWGI*IILRKNIMN CTVTLTY*KGVTSKVLVVISFLLVLFLQMAFQVT CQIR*HTREFPIIIRKPLTN*FLFFYLIF*TSQ GCYHRRVAWRKLTFSFHRHLLNAELRMEVAMVF NERTFPCAGSHYSANSDSKHNSLVKEIVSISQAH PSLARISVSHRAGSK*N*DLQH*GLRLQREGFE *IFSR*IHALMC

Sequence PTK2.eAug05

TCCCGGACCACTGTGAGCCCGCGGCGTGAGGCGTGGGAGGAAGCGCGGCTGCTGTCGCCCAGCGCCGCCCCGTCGTCGTCTGCCTTCGCTTCACGGCGCC GAGCCGCGGTCCGAGCAGAACTGGGGCTCCCTTGCATCTTCCAGTTACAAATTCAGTGCCTTCTGCAGTTTCCCCAGAGCTCCTCAAGAATAACGGAAGG GAGAATATGACAGATACCTAGCATCTAGCAAAATAATGGCAGCTGCTTACCTTGACCCCAACTTGAATCACACACCAAATTCGAGTACATATGAATTGAG AATTCGTTATTTGCCAAAAGGATTTCTAAACCAGTTTACTGAAGATAAGCCAACTTTGAATTTCTTCTATCAACAGGTGAAGAGCGATTATATGTTAGAG ATAGCTGATCAAGTGGACCAGGAAATTGCTTTGAAGTTGGGTTGTCTAGAAATACGGCGATCATACTGGGAGATGCGGGGCAATGCACTAGAAAAGAAGT CTAACTATGAAGTATTAGAAAAAGATGTTGGTTTAAAGCGATTTTTTCCTAAGAGTTTACTGGATTCTGTCAAGGCCAAAACACTAAGAAAACTGATCCA ACAAACATTTAGACAATTTGCCAACCTTAATAGAGAAGAAAGTATTCTGAAATTCTTTGAGATCCTGTCTCCAGTCTACAGATTTGATAAGGAATGCTTC AAGTGTGCTCTTGGTTCAAGCTGGATTATTTCAGTGGAACTGGCAATCGGCCCAGAAGAAGGAATCAGTTACCTAACGGACAAGGGCTGCAATCCCACAC ATCTTGCTGACTTCACTCAAGTGCAAACCATTCAGTATTCAAACAGTGAAGACAAGGACAGAAAAGGAATGCTACAACTAAAAATAGCAGGTGCACCCGA GCCTCTGACAGTGACGGCACCATCCCTAACCATTGCGGAGAATATGGCTGACCTAATAGATGGGTACTGCCGGCTGGTGAATGGAACCTCGCAGTCATTT ATCATCAGACCTCAGAAAGAAGGTGAACGGGCTTTGCCATCAATACCAAAGTTGGCCAACAGCGAAAAGCAAGGCATGCGGACACACGCCGTCTCTGTGT CAGGAGTCAGTCACTGCCAACATAAAGTTAAGAAAGCTAGGCGCTTTCTCCCTTTGGTCTTCTGTTCCCATGATCCTCCTTCTACGGATGAAATTAGTGG GGACGAAACAGATGATTATGCTGAGATTATAGATGAAGAAGATACTTACACCATGCCCTCAAAAAGCTATGGAATAGATGAAGCCAGGGATTATGAGATT CAAAGAGAAAGAATAGAACTTGGACGATGTATTGGAGAAGGCCAATTTGGAGATGTACATCAAGGCATTTATATGAGTCCAGAGAATCCAGCTTTGGCGG TTGCAATTAAAACATGTAAAAACTGTACTTCGGACAGCGTGAGAGAGAAATTTCTTCAAGAAGCCTTAACAATGCGTCAGTTTGACCATCCTCATATTGT GAAGCTGATTGGAGTCATCACAGAGAATCCTGTCTGGATAATCATGGAGCTGTGCACACTTGGAGAGCTGAGGTCATTTTTGCAAGTAAGGAAATACAGT TTGGATCTAGCATCTTTGATCCTGTATGCCTATCAGCTTAGTACAGCTCTTGCATATCTAGAGAGCAAAAGATTTGTACACAGGGACATTGCTGCTCGGA ATGTTCTGGTGTCCTCAAATGATTGTGTAAAATTAGGAGACTTTGGATTATCCCGATATATGGAAGATAGTACTTACTACAAAGCTTCCAAAGGAAAATT GCCTATTAAATGGATGGCTCCAGAGTCAATCAATTTTCGACGTTTTACCTCAGCTAGTGACGTATGGATGTTTGGTGTGTGTATGTGGGAGATACTGATG CATGGTGTGAAGCCTTTTCAAGGAGTGAAGAACAATGATGTAATCGGTCGAATTGAAAATGGGGAAAGATTACCAATGCCTCCAAATTGTCCTCCTACCC TCTACAGCCTTATGACGAAATGCTGGGCCTATGACCCCAGCAGGCGGCCCAGGTTTACTGAACTTAAAGCTCAGCTCAGCACAATCCTGGAGGAAGAGAA GGCTCAGCAAGAAGAGCGCATGAGGATGGAGTCCAGAAGACAGGCCACAGTGTCCTGGGACTCCGGAGGGTCTGATGAAGCACCGCCCAAGCCCAGCAGA CCGGGTTATCCCAGTCCGAGGTCCAGCGAAGGATTTTATCCCAGCCCACAGCACATGGTACAAACCAATCATTACCAGGTTTCTGGCTACCCTGGTTCAC ATGGAATCACAGCCATGGCTGGCAGCATCTATCCAGGTCAGGCATCTCTTTTGGACCAAACAGATTCATGGAATCATAGACCTCAGGAGATAGCAATGTG GCAGCCCAATGTGGAGGACTCTACAGTATTGGACCTGCGAGGGATTGGGCAAGTGTTGCCAACCCATCTGATGGAAGAGCGTCTAATCCGACAGCAACAG GAAATGGAAGAAGATCAGCGCTGGCTGGAAAAAGAGGAAAGATTTCTGAAACCTGATGTGAGACTCTCTCGAGGCAGTATTGACAGGGAGGATGGAAGTC TTCAGGGTCCGATTGGAAACCAACATATATATCAGCCTGTGGGTAAACCAGATCCTGCAGCTCCACCAAAGAAACCGCCTCGCCCTGGAGCTCCCGGTCA TCTGGGAAGCCTTGCCAGCCTCAGCAGCCCTGCTGACAGCTACAACGAGGGTGTCAAGCTTCAGCCCCAGGAAATCAGCCCCCCTCCTACTGCCAACCTG GACCGGTCGAATGATAAGGTGTACGAGAATGTGACGGGCCTGGTGAAAGCTGTCATCGAGATGTCCAGTAAAATCCAGCCAGCCCCACCAGAGGAGTATG TCCCTATGGTGAAGGAAGTCGGCTTGGCCCTGAGGACATTATTGGCCACTGTGGATGAGACCATTCCCCTCCTACCAGCCAGCACCCACCGAGAGATTGA GATGGCACAGAAGCTATTGAACTCTGACCTGGGTGAGCTCATCAACAAGATGAAACTGGCCCAGCAGTATGTCATGACCAGCCTCCAGCAAGAGTACAAA AAGCAAATGCTGACTGCTGCTCACGCCCTGGCTGTGGATGCCAAAAACTTACTCGATGTCATTGACCAAGCAAGACTGAAAATGCTTGGGCAGACGAGAC CACACTGAGCCTCCCCTAGGAGCACGTCTTGCTACCCTCTTTTGAAGATGTTCTCTAGCCTTCCACCAGCAGCGAGGAATTAACCCTGTGTCCTCAGTCG CCAGCACTTACAGCTCCAACTTTTTTGAATGACCATCTGGTTGAAAAATCTTTCTCATATAAGTTTAACCACACTTTGATTTGGGTTCATTTTTTGTTTT GTTTTTTTCAATCATGATATTCAGAAAAATCCAGGATCCAAAATGTGGCGTTTTTCTAAGAATGAAAATTATATGTAAGCTTTTAAGCATCATGAAGAAC AATTTATGTTCACATTAAGATACGTTCTAAAGGGGGATGGCCAAGGGGTGACATCTTAATTCCTAAACTACCTTAGCTGCATAGTGGAAGAGGAGAGCAT GAAGCAAAGAATTCCAGGAAACCCAAGAGGCTGAGAATTCTTTTGTCTACCATAGAATTATTATCCAGACTGGAATTTTTGTTTGTTAGAACACCCTTCA GTTGCAATATGCTAATCCCACTTTACAAAGAATATAAAAGCTATATTTTGAAGACTTGAGTTATTTCAGAAAAAACTACAGCCCTTTTTGTCTTACCTGC CTTTTACTTTCGTGTGGATATGTGAAGCATTGGGTCGGGAACTAGCTGTAGAACACAACTAAAAACTCATGTCTTTTTTCACAGAATAATGTGCCAGTTT TTTGTAGCAATGTTATTTCTCTTGGAAGCAGAAATGCTTTGTACCAGAGCACCTCCAAACTGCATTGAGGAGAAGTTCCAGAACCATCCCCTTTTTCCAT TTTTATATAATTTATAAAGAAAGATTAAAGCCATGTTGACTATTTTACAGCCACTGGAGTTAACTAACCCTTCCTTGTATCTGTCTTCCCAGGAGAGAAT GAAGCAAAACAGGAATTTGGTTTTCTTTTGATGTCCAGTTACACCATCCATTCTGTTAATTTTGAAAAAATATACCCTCCCTTTAGTTTGTTGGGGGATA TAAATTATTCTCAGGAAGAATATAATGAACTGTACAGTTACTTTGACCTATTAAAAAGGTGTTACCAGTAAAGTTCTTGTTGTAATATCCTTTCTTTTGG TTCTGTTTCTTCAGATGGCTTTTCAGGTGACTTGTCAGATAAGATAACATACAAGAGAGTTCCCAATAATTATCAGGAAGCCTCTCACTAATTAGTTTCT TTTTTTTTATTTAATCTTCTAAACAAGTCAAGGATGTTACCACAGAAGGGTAGCGTGGCGTAAGTTAACCTTTTCATTCCATCGTCACCTATTAAATGCA GAGCTCAGGATGGAAGTAGCAATGGTCTTCAATGAAAGAACCTTCCCCTGCGCAGGGTCTCACTATTCCGCTAACTCGGACTCAAAGCATAATTCACTTG TCAAGGAAATTGTTAGCATTTCCCAGGCCCACCCTAGTCTTGCTAGAATCTCTGTGAGCCACAGAGCAGGGAGCAAATAGAACTGAGATCTCCAGCACTA GGGACTCAGACTCCAAAGGGAGGGATTTGAGTGAATTTTTTCCAGGTAAATACATGCTCTTATGTGC

PTK2.au.8.f (forward primer)
PTK2.u.r.53 (reverse primer)
Single exon skipping

Frame 0

SRTTVSPRREAWEEARLLSPSAAPSSSAFASRR RAAVRAELGLPCIFQLQIQCLLQFPQSSSRITEG RI*QIPSI*QNNGSCLP*PQLESHTKFEYI*IE NSLFAKRISKPVY*R*ANFEFLLSTGEERLYVR DS*SSGPGNCFEVGLSRNTAIILGDAGQCTRKEV *L*SIRKRCWFKAIFS*EFTGFCQGQNTKKTDP TNI*TICQP**RRKYSEIL*DPVSSLQI**GML QVCSWFKLDYFSGTGNRPRRRNQLPNGQGLQSHT SC*LHSSANHSVFKQ*RQGQKRNATTKNSRCTR ASDSDGTIPNHCGEYG*PNRWVLPAGEWNLAVI YHQTSERR*TGFAINTKVGQQRKARHADTRRLCV RSQSLPT*S*ES*ALSPFGLLFP*SSFYG*N*W GRNR*LC*DYR*RRYLHHALKKLWNR*SQGL*D SKRKNRTWTMYWRRPIWRCTSRHLYESRESSFGG CN*NM*KLYFGQREREISSRSLNNASV*PSSYC EADWSHHRESCLDNHGAVHTWRAEVIFASKEIQ FGSSIFDPVCLSA*YSSCISREQKICTQGHCCSE CSGVLK*LCKIRRLWIIPIYGR*YLLQSFQRKI AY*MDGSRVNQFSTFYLS**RMDVWCVYVGDTD AWCEAFSRSEEQ*CNRSN*KWGKITNASKLSSYP LQPYDEMLGL*PQQAAQVY*T*SSAQHNPGGRE GSARRAHEDGVQKTGHSVLGLRRV**STAQAQQ TGLSQSEVQRRILSQPTAHGTNQSLPGFWLPWFT WNHSHGWQHLSRSGISFGPNRFMES*TSGDSNV AAQCGGLYSIGPARDWASVANPSDGRASNPTAT GNGRRSALAGKRGKISET*CETLSRQY*QGGWKS SGSDWKPTYISACG*TRSCSSTKETASPWSSRS SGKPCQPQQPC*QLQRGCQASAPGNQPPSYCQP GPVE**GVRECDGPGESCHRDVQ*NPASPTRGVC PYGEGSRLGPEDIIGHCG*DHSPPTSQHPPRD* DGTEAIEL*PG*AHQQDETGPAVCHDQPPARVQ KANADCCSRPGCGCQKLTRCH*PSKTENAWADET TLSLP*EHVLLPSFEDVL*PSTSSEELTLCPQS PALTAPTFLNDHLVEKSFSYKFNHTLIWVHFLF CFFQS*YSEKSRIQNVAFF*E*KLYVSF*AS*RT IYVHIKIRSKGGWPRGDILIPKLP*LHSGRGEH EAKNSRKPKRLRILLSTIELLSRLEFLFVRTPF SCNMLIPLYKEYKSYILKT*VISEKTTALFVLPA FYFRVDM*SIGSGTSCRTQLKTHVFFHRIMCQF FVAMLFLLEAEMLCTRAPPNCIEEKFQNHPLFP FLYNL*RKIKAMLTILQPLELTNPSLYLSSQERM KQNRNLVFF*CPVTPSILLILKKYTLPLVCWGI *IILRKNIMNCTVTLTY*KGVTSKVLVVISFLL VLFLQMAFQVTCQIR*HTREFPIIIRKPLTN*FL FFYLIF*TSQGCYHRRVAWRKLTFSFHRHLLNA ELRMEVAMVFNERTFPCAGSHYSANSDSKHNSL VKEIVSISQAHPSLARISVSHRAGSK*N*DLQH* GLRLQREGFE*IFSR*IHALMC

Frame 1

PGPL*ARGVRRGRKRGCCRPAPPRRRLPSLHGA EPRSEQNWGSLASSSYKFSAFCSFPRAPQE*RK GEYDRYLASSKIMAAAYLDPNLNHTPNSSTYELR IRYLPKGFLNQFTEDKPTLNFFYQQVKSDYMLE IADQVDQEIALKLGCLEIRRSYWEMRGNALEKK SNYEVLEKDVGLKRFFPKSLLDSVKAKTLRKLIQ QTFRQFANLNREESILKFFEILSPVYRFDKECF KCALGSSWIISVELAIGPEEGISYLTDKGCNPT HLADFTQVQTIQYSNSEDKDRKGMLQLKIAGAPE PLTVTAPSLTIAENMADLIDGYCRLVNGTSQSF IIRPQKEGERALPSIPKLANSEKQGMRTHAVSV SGVSHCQHKVKKARRFLPLVFCSHDPPSTDEISG DETDDYAEIIDEEDTYTMPSKSYGIDEARDYEI QRERIELGRCIGEGQFGDVHQGIYMSPENPALA VAIKTCKNCTSDSVREKFLQEALTMRQFDHPHIV KLIGVITENPVWIIMELCTLGELRSFLQVRKYS LDLASLILYAYQLSTALAYLESKRFVHRDIAAR NVLVSSNDCVKLGDFGLSRYMEDSTYYKASKGKL PIKWMAPESINFRRFTSASDVWMFGVCMWEILM HGVKPFQGVKNNDVIGRIENGERLPMPPNCPPT LYSLMTKCWAYDPSRRPRFTELKAQLSTILEEEK AQQEERMRMESRRQATVSWDSGGSDEAPPKPSR PGYPSPRSSEGFYPSPQHMVQTNHYQVSGYPGS HGITAMAGSIYPGQASLLDQTDSWNHRPQEIAMW QPNVEDSTVLDLRGIGQVLPTHLMEERLIRQQQ EMEEDQRWLEKEERFLKPDVRLSRGSIDREDGS LQGPIGNQHIYQPVGKPDPAAPPKKPPRPGAPGH LGSLASLSSPADSYNEGVKLQPQEISPPPTANL DRSNDKVYENVTGLVKAVIEMSSKIQPAPPEEY VPMVKEVGLALRTLLATVDETIPLLPASTHREIE MAQKLLNSDLGELINKMKLAQQYVMTSLQQEYK KQMLTAAHALAVDAKNLLDVIDQARLKMLGQTR PH*ASPRSTSCYPLLKMFSSLPPAARN*PCVLSR QHLQLQLF*MTIWLKNLSHISLTTL*FGFIFCF VFFNHDIQKNPGSKMWRFSKNENYM*AFKHHEE QFMFTLRYVLKGDGQGVTS*FLNYLSCIVEEESM KQRIPGNPRG*EFFCLP*NYYPDWNFCLLEHPS VAIC*SHFTKNIKAIF*RLELFQKKLQPFLSYL PFTFVWICEALGRELAVEHN*KLMSFFTE*CASF L*QCYFSWKQKCFVPEHLQTALRRSSRTIPFFH FYIIYKERLKPC*LFYSHWS*LTLPCICLPRRE *SKTGIWFSFDVQLHHPFC*F*KNIPSL*FVGGY KLFSGRI**TVQLL*PIKKVLPVKFLL*YPFFW FCFFRWLFR*LVR*DNIQESSQ*LSGSLSLISF FFFI*SSKQVKDVTTEG*RGVS*PFHSIVTY*MQ SSGWK*QWSSMKEPSPAQGLTIPLTRTQSIIHL SRKLLAFPRPTLVLLESL*ATEQGANRTEISST RDSDSKGRDLSEFFPGKYMLLC

Frame 2

PDHCEPAA*GVGGSAAAVAQRRPVVVCLRFTAP SRGPSRTGAPLHLPVTNSVPSAVSPELLKNNGR ENMTDT*HLAK*WQLLTLTPT*ITHQIRVHMN* EFVICQKDF*TSLLKISQL*ISSINR*RAIIC*R *LIKWTRKLL*SWVV*KYGDHTGRCGAMH*KRS LTMKY*KKMLV*SDFFLRVYWILSRPKH*EN*S NKHLDNLPTLIEKKVF*NSLRSCLQSTDLIRNAS SVLLVQAGLFQWNWQSAQKKESVT*RTRAAIPH ILLTSLKCKPFSIQTVKTRTEKECYN*K*QVHP SL*Q*RHHP*PLRRIWLT**MGTAGW*MEPRSHL SSDLRKKVNGLCHQYQSWPTAKSKACGHTPSLC QESVTANIKLRKLGAFSLWSSVPMILLLRMKLV GTKQMIMLRL*MKKILTPCPQKAME*MKPGIMRF KEKE*NLDDVLEKANLEMYIKAFI*VQRIQLWR LQLKHVKTVLRTA*ERNFFKKP*QCVSLTILIL *S*LESSQRILSG*SWSCAHLES*GHFCK*GNTV WI*HL*SCMPISLVQLLHI*RAKDLYTGTLLLG MFWCPQMIV*N*ETLDYPDIWKIVLTTKLPKEN CLLNGWLQSQSIFDVLPQLVTYGCLVCVCGRY*C MV*SLFKE*RTMM*SVELKMGKDYQCLQIVLLP STAL*RNAGPMTPAGGPGLLNLKLSSAQSWRKR RLSKKSA*GWSPEDRPQCPGTPEGLMKHRPSPAD RVIPVRGPAKDFIPAHSTWYKPIITRFLATLVH MESQPWLAASIQVRHLFWTKQIHGIIDLRR*QC GSPMWRTLQYWTCEGLGKCCQPI*WKSV*SDSNR KWKKISAGWKKRKDF*NLM*DSLEAVLTGRMEV FRVRLETNIYISLWVNQILQLHQRNRLALELPV IWEALPASAALLTATTRVSSFSPRKSAPLLLPTW TGRMIRCTRM*RAW*KLSSRCPVKSSQPHQRSM SLW*RKSAWP*GHYWPLWMRPFPSYQPAPTERL RWHRSY*TLTWVSSSTR*NWPSSMS*PASSKSTK SKC*LLLTPWLWMPKTYSMSLTKQD*KCLGRRD HTEPPLGARLATLF*RCSLAFHQQRGINPVSSV ASTYSSNFFE*PSG*KIFLI*V*PHFDLGSFFVL FFSIMIFRKIQDPKCGVFLRMKIICKLLSIMKN NLCSH*DTF*RGMAKG*HLNS*TTLAA*WKRRA *SKEFQETQEAENSFVYHRIIIQTGIFVC*NTLQ LQYANPTLQRI*KLYFEDLSYFRKNYSPFCLTC LLLSCGYVKHWVGN*L*NTTKNSCLFSQNNVPV FCSNVISLGSRNALYQSTSKLH*GEVPEPSPFSI FI*FIKKD*SHVDYFTATGVN*PFLVSVFPGEN EAKQEFGFLLMSSYTIHSVNFEKIYPPFSLLGD INYSQEEYNELYSYFDLLKRCYQ*SSCCNILSFG SVSSDGFSGDLSDKITYKRVPNNYQEASH*LVS FFLFNLLNKSRMLPQKGSVA*VNLFIPSSPIKC RAQDGSSNGLQ*KNLPLRRVSLFR*LGLKA*FTC QGNC*HFPGPP*SC*NLCEPQSREQIELRSPAL GTQTPKGGI*VNFFQVNTCSYV

Sequence PTK2.dAug05

AGGAGGGGAGGCCGGTGGCCGTGAGGAGGTTGGGGGCCCTCGCGGAGGCCGAGAGGCTTAAGGAGGCAGAACTGGGGCTCCCTTGCATCTTCCAGTTACA AATTCAGTGCCTTCTGCAGTTTCCCCAGAGCTCCTCAAGAATAACGGAAGGGAGGTATAGTAATGGTTAAGAAACTTTGCAAAGAAAGTGAATAATGCCA GTACTGTCACCTTTTGGAACTTGTTGGTATTTCCTGTTGTCTGTCTTTGGAAGAATATGACAGATACCTAGCATCTAGCAAAATAATGGCAGCTGCTTAC CTTGACCCCAACTTGAATCACACACCAAATTCGAGTACTAAGACTCACCTGGGTACTGGTATGGAACGTTCTCCTGGTGCAATGGAGCGAGTATTAAAGG TCTTTCATTATTTTGAAAGCAATAGTGAGCCAACCACCTGGGCCAGTATTATCAGGCATGGAGATGCTACTGATGTCAGGGGCATCATTCAGAAGATAGT GGACAGTCACAAAGTAAAGCATGTGGCCTGCTATGGATTCCGCCTCAGTCACCTGCGGTCAGAGGAGGTTCACTGGCTTCACGTGGATATGGGCGTCTCC AGTGTGAGGGAGAAGTATGAGCTTGCTCACCCACCAGAGGAGTGGAAATATGAATTGAGAATTCGTTATTTGCCAAAAGGATTTCTAAACCAGTTTACTG AAGATAAGCCAACTTTGAATTTCTTCTATCAACAGGTGAAGAGCGATTATATGTTAGAGATAGCTGATCAAGTGGACCAGGAAATTGCTTTGAAGTTGGG TTGTCTAGAAATACGGCGATCATACTGGGAGATGCGGGGCAATGCACTAGAAAAGAAGTCTAACTATGAAGTATTAGAAAAAGATGTTGGTTTAAAGCGA TTTTTTCCTAAGAGTTTACTGGATTCTGTCAAGGCCAAAACACTAAGAAAACTGATCCAACAAACATTTAGACAATTTGCCAACCTTAATAGAGAAGAAA GTATTCTGAAATTCTTTGAGATCCTGTCTCCAGTCTACAGATTTGATAAGGAATGCTTCAAGTGTGCTCTTGGTTCAAGCTGGATTATTTCAGTGGAACT GGCAATCGGCCCAGAAGAAGGAATCAGTTACCTAACGGACAAGGGCTGCAATCCCACACATCTTGCTGACTTCACTCAAGTGCAAACCATTCAGTATTCA AACAGTGAAGACAAGGACAGAAAAGGAATGCTACAACTAAAAATAGCAGGTGCACCCGAGCCTCTGACAGTGACGGCACCATCCCTAACCATTGCGGAGA ATATGGCTGACCTAATAGATGGGTACTGCCGGCTGGTGAATGGAACCTCGCAGTCATTTATCATCAGACCTCAGAAAGAAGGTGAACGGGCTTTGCCATC AATACCAAAGTTGGCCAACAGCGAAAAGCAAGGCATGCGGACACACGCCGTCTCTGTGTCAGAAACAGATGATTATGCTGAGATTATAGATGAAGAAGAT ACTTACACCATGCCCTCAACCAGGGATTATGAGATTCAAAGAGAAAGAATAGAACTTGGACGATGTATTGGAGAAGGCCAATTTGGAGATGTACATCAAG GCATTTATATGAGTCCAGAGAATCCAGCTTTGGCGGTTGCAATTAAAACATGTAAAAACTGTACTTCGGACAGCGTGAGAGAGAAATTTCTTCAAGAAGC CTTAACAATGCGTCAGTTTGACCATCCTCATATTGTGAAGCTGATTGGAGTCATCACAGAGAATCCTGTCTGGATAATCATGGAGCTGTGCACACTTGGA GAGCTGAGGTCATTTTTGCAAGTAAGGAAATACAGTTTGGATCTAGCATCTTTGATCCTGTATGCCTATCAGCTTAGTACAGCTCTTGCATATCTAGAGA GCAAAAGATTTGTACACAGGGACATTGCTGCTCGGAATGTTCTGGTGTCCTCAAATGATTGTGTAAAATTAGGAGACTTTGGATTATCCCGATATATGGA AGATAGTACTTACTACAAAGCTTCCAAAGGAAAATTGCCTATTAAATGGATGGCTCCAGAGTCAATCAATTTTCGACGTTTTACCTCAGCTAGTGACGTA TGGATGTTTGGTGTGTGTATGTGGGAGATACTGATGCATGGTGTGAAGCCTTTTCAAGGAGTGAAGAACAATGATGTAATCGGTCGAATTGAAAATGGGG AAAGATTACCAATGCCTCCAAATTGTCCTCCTACCCTCTACAGCCTTATGACGAAATGCTGGGCCTATGACCCCAGCAGGCGGCCCAGGTTTACTGAACT TAAAGCTCAGCTCAGCACAATCCTGGAGGAAGAGAAGGCTCAGCAAGAAGAGCGCATGAGGATGGAGTCCAGAAGACAGGCCACAGTGTCCTGGGACTCC GGAGGGTCTGATGAAGCACCGCCCAAGCCCAGCAGACCGGGTTATCCCAGTCCGAGGTCCAGCGAAGGATTTTATCCCAGCCCACAGCACATGGTACAAA CCAATCATTACCAGGACTCTACAGTATTGGACCTGCGAGGGATTGGGCAAGTGTTGCCAACCCATCTGATGGAAGAGCGTCTAATCCGACAGCAACAGGA AATGGAAGAAGATCAGCGCTGGCTGGAAAAAGAGGAAAGATTTCTGAAACCTGATGTGAGACTCTCTCGAGGCAGTATTGACAGGGAGGATGGAAGTCTT CAGGGTCCGATTGGAAACCAACATATATATCAGCCTGTGGGTAAACCAGATCCTGCAGCTCCACCAAAGAAACCGCCTCGCCCTGGAGCTCCCGGTCATC TGGGAAGCCTTGCCAGCCTCAGCAGCCCTGCTGACAGCTACAACGAGGGTGTCAAGCTTCAGCCCCAGGAAATCAGCCCCCCTCCTACTGCCAACCTGGA CCGGTCGAATGATAAGGTGTACGAGAATGTGACGGGCCTGGTGAAAGCTGTCATCGAGATGTCCAGTAAAATCCAGCCAGCCCCACCAGAGGAGTATGTC CCTATGGTGAAGGAAGTCGGCTTGGCCCTGAGGACATTATTGGCCACTGTGGATGAGACCATTCCCCTCCTACCAGCCAGCACCCACCGAGAGATTGAGA TGGCACAGAAGCTATTGAACTCTGACCTGGGTGAGCTCATCAACAAGATGAAACTGGCCCAGCAGTATGTCATGACCAGCCTCCAGCAAGAGTACAAAAA GCAAATGCTGACTGCTGCTCACGCCCTGGCTGTGGATGCCAAAAACTTACTCGATGTCATTGACCAAGCAAGACTGAAAATGCTTGGGCAGACGAGACCA CACTGAGCCTCCCCTAGGAGCACGTCTTGCTACCCTCTTTTGAAGATGTTCTCTAGCCTTCCACCAGCAGCGAGGAATTAACCCTGTGTCCTCAGTCGCC AGCACTTACAGCTCCAACTTTTTTGAATGACCATCTGGTTGAAAAATCTTTCTCATATAAGTTTAACCACACTTTGATTTGGGTTCATTTTTTGTTTTGT TTTTTTCAATCATGATATTCAGAAAAATCCAGGATCCAAAATGTGGCGTTTTTCTAAGAATGAAAATTATATGTAAGCTTTTAAGCATCATGAAGAACAA TTTATGTTCACATTAAGATACGTTCTAAAGGGGGATGGCCAAGGGGTGACATCTTAATTCCTAAACTACCTTAGCTGCATAGTGGAAGAGGAGAGCATGA AGCAAAGAATTCCAGGAAACCCAAGAGGCTGAGAATTCTTTTGTCTACCATAGAATTATTATCCAGACTGGAATTTTTGTTTGTTAGAACACCCTTCAGT TGCAATATGCTAATCCCACTTTACAAAGAATATAAAAGCTATATTTTGAAGACTTGAGTTATTTCAGAAAAAACTACAGCCCTTTTTGTCTTACCTGCCT TTTACTTTCGTGTGGATATGTGAAGCATTGGGTCGGGAACTAGCTGTAGAACACAACTAAAAACTCATGTCTTTTTTCACAGAATAATGTGCCAGTTTTT TGTAGCAATGTTATTTCTCTTGGAAGCAGAAATGCTTTGTACCAGAGCACCTCCAAACTGCATTGAGGAGAAGTTCCAGAACCATCCCCTTTTTCCATTT TTATATAATTTATAAAGAAAGATTAAAGCCATGTTGACTATTTTACAGCCACTGGAGTTAACTAACCCTTCCTTGTATCTGTCTTCCCAGGAGAGAATGA AGCAAAACAGGAATTTGGTTTTCTTTTGATGTCCAGTTACACCATCCATTCTGTTAATTTTGAAAAAATATACCCTCCCTTTAGTTTGTTGGGGGATATA AATTATTCTCAGGAAGAATATAATGAACTGTACAGTTACTTTGACCTATTAAAAAGGTGTTACCAGTAAAGTTCTTGTTGTAATATCCTTTCTTTTGGTT CTGTTTCTTCAGATGGCTTTTCAGGTGACTTGTCAGATAAGATAACATACAAGAGAGTTCCCAATAATTATCAGGAAGCCTCTCACTAATTAGTTTCTTT TTTTTTATTTAATCTTCTAAACAAGTCAAGGATGTTACCACAGAAGGGTAGCGTGGCGTAAGTTAACCTTTTCATTCCATCGTCACCTATTAAATGCAGA GCTCAGGATGGAAGTAGCAATGGTCTTCAATGAAAGAACCTTCCCCTGCGCAGGGTCTCACTATTCCGCTAACTCGGACTCAAAGCATAATTCACTTGTC AAGGAAATTGTTAGCATTTCCCAGGCCCACCCTAGTCTTGCTAGAATCTCTGTGAGCCACAGAGCAGGGAGCAAATAGAACTGAGATCTCCAGCACTAGG GACTCAGACTCCAAAGGGAGGGATTTGAGTGAATTTTTTCCAGGTAAATACATGCTCTTATGTGC

PTK2.au.8.f (forward primer)
PTK2.u.r.53 (reverse primer)
Single exon skipping

Frame 0

RRGGRWP*GGWGPSRRPRGLRRQNWGSLASSSY KFSAFCSFPRAPQE*RKGGIVMVKKLCKESE*CQ YCHLLELVGISCCLSLEEYDRYLASSKIMAAAY LDPNLNHTPNSSTKTHLGTGMERSPGAMERVLK VFHYFESNSEPTTWASIIRHGDATDVRGIIQKIV DSHKVKHVACYGFRLSHLRSEEVHWLHVDMGVS SVREKYELAHPPEEWKYELRIRYLPKGFLNQFT EDKPTLNFFYQQVKSDYMLEIADQVDQEIALKLG CLEIRRSYWEMRGNALEKKSNYEVLEKDVGLKR FFPKSLLDSVKAKTLRKLIQQTFRQFANLNREE SILKFFEILSPVYRFDKECFKCALGSSWIISVEL AIGPEEGISYLTDKGCNPTHLADFTQVQTIQYS NSEDKDRKGMLQLKIAGAPEPLTVTAPSLTIAE NMADLIDGYCRLVNGTSQSFIIRPQKEGERALPS IPKLANSEKQGMRTHAVSVSETDDYAEIIDEED TYTMPSTRDYEIQRERIELGRCIGEGQFGDVHQ GIYMSPENPALAVAIKTCKNCTSDSVREKFLQEA LTMRQFDHPHIVKLIGVITENPVWIIMELCTLG ELRSFLQVRKYSLDLASLILYAYQLSTALAYLE SKRFVHRDIAARNVLVSSNDCVKLGDFGLSRYME DSTYYKASKGKLPIKWMAPESINFRRFTSASDV WMFGVCMWEILMHGVKPFQGVKNNDVIGRIENG ERLPMPPNCPPTLYSLMTKCWAYDPSRRPRFTEL KAQLSTILEEEKAQQEERMRMESRRQATVSWDS GGSDEAPPKPSRPGYPSPRSSEGFYPSPQHMVQ TNHYQDSTVLDLRGIGQVLPTHLMEERLIRQQQE MEEDQRWLEKEERFLKPDVRLSRGSIDREDGSL QGPIGNQHIYQPVGKPDPAAPPKKPPRPGAPGH LGSLASLSSPADSYNEGVKLQPQEISPPPTANLD RSNDKVYENVTGLVKAVIEMSSKIQPAPPEEYV PMVKEVGLALRTLLATVDETIPLLPASTHREIE MAQKLLNSDLGELINKMKLAQQYVMTSLQQEYKK QMLTAAHALAVDAKNLLDVIDQARLKMLGQTRP H*ASPRSTSCYPLLKMFSSLPPAARN*PCVLSR QHLQLQLF*MTIWLKNLSHISLTTL*FGFIFCFV FFNHDIQKNPGSKMWRFSKNENYM*AFKHHEEQ FMFTLRYVLKGDGQGVTS*FLNYLSCIVEEESM KQRIPGNPRG*EFFCLP*NYYPDWNFCLLEHPSV AIC*SHFTKNIKAIF*RLELFQKKLQPFLSYLP FTFVWICEALGRELAVEHN*KLMSFFTE*CASF L*QCYFSWKQKCFVPEHLQTALRRSSRTIPFFHF YIIYKERLKPC*LFYSHWS*LTLPCICLPRRE* SKTGIWFSFDVQLHHPFC*F*KNIPSL*FVGGY KLFSGRI**TVQLL*PIKKVLPVKFLL*YPFFWF CFFRWLFR*LVR*DNIQESSQ*LSGSLSLISFF FFI*SSKQVKDVTTEG*RGVS*PFHSIVTY*MQ SSGWK*QWSSMKEPSPAQGLTIPLTRTQSIIHLS RKLLAFPRPTLVLLESL*ATEQGANRTEISSTR DSDSKGRDLSEFFPGKYMLLC

Frame 1

GGEAGGREEVGGPRGGREA*GGRTGAPLHLPVT NSVPSAVSPELLKNNGREV**WLRNFAKKVNNA STVTFWNLLVFPVVCLWKNMTDT*HLAK*WQLLT LTPT*ITHQIRVLRLTWVLVWNVLLVQWSEY*R SFIILKAIVSQPPGPVLSGMEMLLMSGASFRR* WTVTK*SMWPAMDSASVTCGQRRFTGFTWIWASP V*GRSMSLLTHQRSGNMN*EFVICQKDF*TSLL KISQL*ISSINR*RAIIC*R*LIKWTRKLL*SW VV*KYGDHTGRCGAMH*KRSLTMKY*KKMLV*SD FFLRVYWILSRPKH*EN*SNKHLDNLPTLIEKK VF*NSLRSCLQSTDLIRNASSVLLVQAGLFQWN WQSAQKKESVT*RTRAAIPHILLTSLKCKPFSIQ TVKTRTEKECYN*K*QVHPSL*Q*RHHP*PLRR IWLT**MGTAGW*MEPRSHLSSDLRKKVNGLCH QYQSWPTAKSKACGHTPSLCQKQMIMLRL*MKKI LTPCPQPGIMRFKEKE*NLDDVLEKANLEMYIK AFI*VQRIQLWRLQLKHVKTVLRTA*ERNFFKK P*QCVSLTILIL*S*LESSQRILSG*SWSCAHLE S*GHFCK*GNTVWI*HL*SCMPISLVQLLHI*R AKDLYTGTLLLGMFWCPQMIV*N*ETLDYPDIW KIVLTTKLPKENCLLNGWLQSQSIFDVLPQLVTY GCLVCVCGRY*CMV*SLFKE*RTMM*SVELKMG KDYQCLQIVLLPSTAL*RNAGPMTPAGGPGLLN LKLSSAQSWRKRRLSKKSA*GWSPEDRPQCPGTP EGLMKHRPSPADRVIPVRGPAKDFIPAHSTWYK PIITRTLQYWTCEGLGKCCQPI*WKSV*SDSNR KWKKISAGWKKRKDF*NLM*DSLEAVLTGRMEVF RVRLETNIYISLWVNQILQLHQRNRLALELPVI WEALPASAALLTATTRVSSFSPRKSAPLLLPTW TGRMIRCTRM*RAW*KLSSRCPVKSSQPHQRSMS LW*RKSAWP*GHYWPLWMRPFPSYQPAPTERLR WHRSY*TLTWVSSSTR*NWPSSMS*PASSKSTK SKC*LLLTPWLWMPKTYSMSLTKQD*KCLGRRDH TEPPLGARLATLF*RCSLAFHQQRGINPVSSVA STYSSNFFE*PSG*KIFLI*V*PHFDLGSFFVL FFSIMIFRKIQDPKCGVFLRMKIICKLLSIMKNN LCSH*DTF*RGMAKG*HLNS*TTLAA*WKRRA* SKEFQETQEAENSFVYHRIIIQTGIFVC*NTLQ LQYANPTLQRI*KLYFEDLSYFRKNYSPFCLTCL LLSCGYVKHWVGN*L*NTTKNSCLFSQNNVPVF CSNVISLGSRNALYQSTSKLH*GEVPEPSPFSI FI*FIKKD*SHVDYFTATGVN*PFLVSVFPGENE AKQEFGFLLMSSYTIHSVNFEKIYPPFSLLGDI NYSQEEYNELYSYFDLLKRCYQ*SSCCNILSFG SVSSDGFSGDLSDKITYKRVPNNYQEASH*LVSF FLFNLLNKSRMLPQKGSVA*VNLFIPSSPIKCR AQDGSSNGLQ*KNLPLRRVSLFR*LGLKA*FTC QGNC*HFPGPP*SC*NLCEPQSREQIELRSPALG TQTPKGGI*VNFFQVNTCSYV

Frame 2

EGRPVAVRRLGALAEAERLKEAELGLPCIFQLQ IQCLLQFPQSSSRITEGRYSNG*ETLQRK*IMP VLSPFGTCWYFLLSVFGRI*QIPSI*QNNGSCL P*PQLESHTKFEY*DSPGYWYGTFSWCNGASIKG LSLF*KQ**ANHLGQYYQAWRCY*CQGHHSEDS GQSQSKACGLLWIPPQSPAVRGGSLASRGYGRL QCEGEV*ACSPTRGVEI*IENSLFAKRISKPVY* R*ANFEFLLSTGEERLYVRDS*SSGPGNCFEVG LSRNTAIILGDAGQCTRKEV*L*SIRKRCWFKA IFS*EFTGFCQGQNTKKTDPTNI*TICQP**RRK YSEIL*DPVSSLQI**GMLQVCSWFKLDYFSGT GNRPRRRNQLPNGQGLQSHTSC*LHSSANHSVF KQ*RQGQKRNATTKNSRCTRASDSDGTIPNHCGE YG*PNRWVLPAGEWNLAVIYHQTSERR*TGFAI NTKVGQQRKARHADTRRLCVRNR*LC*DYR*RR YLHHALNQGL*DSKRKNRTWTMYWRRPIWRCTSR HLYESRESSFGGCN*NM*KLYFGQREREISSRS LNNASV*PSSYCEADWSHHRESCLDNHGAVHTW RAEVIFASKEIQFGSSIFDPVCLSA*YSSCISRE QKICTQGHCCSECSGVLK*LCKIRRLWIIPIYG R*YLLQSFQRKIAY*MDGSRVNQFSTFYLS**R MDVWCVYVGDTDAWCEAFSRSEEQ*CNRSN*KWG KITNASKLSSYPLQPYDEMLGL*PQQAAQVY*T *SSAQHNPGGREGSARRAHEDGVQKTGHSVLGL RRV**STAQAQQTGLSQSEVQRRILSQPTAHGTN QSLPGLYSIGPARDWASVANPSDGRASNPTATG NGRRSALAGKRGKISET*CETLSRQY*QGGWKS SGSDWKPTYISACG*TRSCSSTKETASPWSSRSS GKPCQPQQPC*QLQRGCQASAPGNQPPSYCQPG PVE**GVRECDGPGESCHRDVQ*NPASPTRGVC PYGEGSRLGPEDIIGHCG*DHSPPTSQHPPRD*D GTEAIEL*PG*AHQQDETGPAVCHDQPPARVQK ANADCCSRPGCGCQKLTRCH*PSKTENAWADET TLSLP*EHVLLPSFEDVL*PSTSSEELTLCPQSP ALTAPTFLNDHLVEKSFSYKFNHTLIWVHFLFC FFQS*YSEKSRIQNVAFF*E*KLYVSF*AS*RT IYVHIKIRSKGGWPRGDILIPKLP*LHSGRGEHE AKNSRKPKRLRILLSTIELLSRLEFLFVRTPFS CNMLIPLYKEYKSYILKT*VISEKTTALFVLPA FYFRVDM*SIGSGTSCRTQLKTHVFFHRIMCQFF VAMLFLLEAEMLCTRAPPNCIEEKFQNHPLFPF LYNL*RKIKAMLTILQPLELTNPSLYLSSQERM KQNRNLVFF*CPVTPSILLILKKYTLPLVCWGI* IILRKNIMNCTVTLTY*KGVTSKVLVVISFLLV LFLQMAFQVTCQIR*HTREFPIIIRKPLTN*FL FFYLIF*TSQGCYHRRVAWRKLTFSFHRHLLNAE LRMEVAMVFNERTFPCAGSHYSANSDSKHNSLV KEIVSISQAHPSLARISVSHRAGSK*N*DLQH* GLRLQREGFE*IFSR*IHALMC

Sequence PTK2.cAug05

TCCCGGACCACTGTGAGCCCGCGGCGTGAGGCGTGGGAGGAAGCGCGGCTGCTGTCGCCCAGCGCCGCCCCGTCGTCGTCTGCCTTCGCTTCACGGCGCC GAGCCGCGGTCCGAGCAGAACTGGGGCTCCCTTGCATCTTCCAGTTACAAATTCAGTGCCTTCTGCAGTTTCCCCAGAGCTCCTCAAGAATAACGGAAGG GAGAATATGACAGATACCTAGCATCTAGCAAAATAATGGCAGCTGCTTACCTTGACCCCAACTTGAATCACACACCAAATTCGAGTACTAAGACTCACCT GGGTACTGGTATGGAACGTTCTCCTGGTGCAATGGAGCGAGTATTAAAGGTCTTTCATTATTTTGAAAGCAATAGTGAGCCAACCACCTGGGCCAGTATT ATCAGGCATGGAGATGCTACTGATGTCAGGGGCATCATTCAGAAGATAGTGGACAGTCACAAAGTAAAGCATGTGGCCTGCTATGGATTCCGCCTCAGTC ACCTGCGGTCAGAGGAGGTTCACTGGCTTCACGTGGATATGGGCGTCTCCAGTGTGAGGGAGAAGTATGAGCTTGCTCACCCACCAGAGGAGTGGAAATA TGAATTGAGAATTCGTTATTTGCCAAAAGGATTTCTAAACCAGTTTACTGAAGATAAGCCAACTTTGAATTTCTTCTATCAACAGGTGAAGAGCGATTAT ATGTTAGAGATAGCTGATCAAGTGGACCAGGAAATTGCTTTGAAGTTGGGTTGTCTAGAAATACGGCGATCATACTGGGAGATGCGGGGCAATGCACTAG AAAAGAAGTCTAACTATGAAGTATTAGAAAAAGATGTTGGTTTAAAGCGATTTTTTCCTAAGAGTTTACTGGATTCTGTCAAGGCCAAAACACTAAGAAA ACTGATCCAACAAACATTTAGACAATTTGCCAACCTTAATAGAGAAGAAAGTATTCTGAAATTCTTTGAGATCCTGTCTCCAGTCTACAGATTTGATAAG GAATGCTTCAAGTGTGCTCTTGGTTCAAGCTGGATTATTTCAGTGGAACTGGCAATCGGCCCAGAAGAAGGAATCAGTTACCTAACGGACAAGGGCTGCA ATCCCACACATCTTGCTGACTTCACTCAAGTGCAAACCATTCAGTATTCAAACAGTGAAGACAAGGACAGAAAAGGAATGCTACAACTAAAAATAGCAGG TGCACCCGAGCCTCTGACAGTGACGGCACCATCCCTAACCATTGCGGAGAATATGGCTGACCTAATAGATGGGTACTGCCGGCTGGTGAATGGAACCTCG CAGTCATTTATCATCAGACCTCAGAAAGAAGGTGAACGGGCTTTGCCATCAATACCAAAGTTGGCCAACAGCGAAAAGCAAGGCATGCGGACACACGCCG TCTCTGTGTCAGAAACAGATGATTATGCTGAGATTATAGATGAAGAAGATACTTACACCATGCCCTCAACCAGGGATTATGAGATTCAAAGAGAAAGAAT AGAACTTGGACGATGTATTGGAGAAGGCCAATTTGGAGATGTACATCAAGGCATTTATATGAGTCCAGAGAATCCAGCTTTGGCGGTTGCAATTAAAACA TGTAAAAACTGTACTTCGGACAGCGTGAGAGAGAAATTTCTTCAAGAAGCCTTAACAATGCGTCAGTTTGACCATCCTCATATTGTGAAGCTGATTGGAG TCATCACAGAGAATCCTGTCTGGATAATCATGGAGCTGTGCACACTTGGAGAGCTGAGGTCATTTTTGCAAGTAAGGAAATACAGTTTGGATCTAGCATC TTTGATCCTGTATGCCTATCAGCTTAGTACAGCTCTTGCATATCTAGAGAGCAAAAGATTTGTACACAGGGACATTGCTGCTCGGAATGTTCTGGTGTCC TCAAATGATTGTGTAAAATTAGGAGACTTTGGATTATCCCGATATATGGAAGATAGTACTTACTACAAAGCTTCCAAAGGAAAATTGCCTATTAAATGGA TGGCTCCAGAGTCAATCAATTTTCGACGTTTTACCTCAGCTAGTGACGTATGGATGTTTGGTGTGTGTATGTGGGAGATACTGATGCATGGTGTGAAGCC TTTTCAAGGAGTGAAGAACAATGATGTAATCGGTCGAATTGAAAATGGGGAAAGATTACCAATGCCTCCAAATTGTCCTCCTACCCTCTACAGCCTTATG ACGAAATGCTGGGCCTATGACCCCAGCAGGCGGCCCAGGTTTACTGAACTTAAAGCTCAGCTCAGCACAATCCTGGAGGAAGAGAAGGCTCAGCAAGAAG AGCGCATGAGGATGGAGTCCAGAAGACAGGCCACAGTGTCCTGGGACTCCGGAGGGTCTGATGAAGCACCGCCCAAGCCCAGCAGACCGGGTTATCCCAG TCCGAGGTCCAGCGAAGGATTTTATCCCAGCCCACAGCACATGGTACAAACCAATCATTACCAGGTTTCTGGCTACCCTGGTTCACATGGAATCACAGCC ATGGCTGGCAGCATCTATCCAGGTCAGGCATCTCTTTTGGACCAAACAGATTCATGGAATCATAGACCTCAGGAGATAGCAATGTGGCAGCCCAATGTGG AGGACTCTACAGTATTGGACCTGCGAGGGATTGGGCAAGTGTTGCCAACCCATCTGATGGAAGAGCGTCTAATCCGACAGCAACAGGAAATGGAAGAAGA TCAGCGCTGGCTGGAAAAAGAGGAAAGATTTCTGAAACCTGATGTGAGACTCTCTCGAGGCAGTATTGACAGGGAGGATGGAAGTCTTCAGGGTCCGATT GGAAACCAACATATATATCAGCCTGTGGGTAAACCAGATCCTGCAGCTCCACCAAAGAAACCGCCTCGCCCTGGAGCTCCCGGTCATCTGGGAAGCCTTG CCAGCCTCAGCAGCCCTGCTGACAGCTACAACGAGGGTGTCAAGCTTCAGCCCCAGGAAATCAGCCCCCCTCCTACTGCCAACCTGGACCGGTCGAATGA TAAGGTGTACGAGAATGTGACGGGCCTGGTGAAAGCTGTCATCGAGATGTCCAGTAAAATCCAGCCAGCCCCACCAGAGGAGTATGTCCCTATGGTGAAG GAAGTCGGCTTGGCCCTGAGGACATTATTGGCCACTGTGGATGAGACCATTCCCCTCCTACCAGCCAGCACCCACCGAGAGATTGAGATGGCACAGAAGC TATTGAACTCTGACCTGGGTGAGCTCATCAACAAGATGAAACTGGCCCAGCAGTATGTCATGACCAGCCTCCAGCAAGAGTACAAAAAGCAAATGCTGAC TGCTGCTCACGCCCTGGCTGTGGATGCCAAAAACTTACTCGATGTCATTGACCAAGCAAGACTGAAAATGCTTGGGCAGACGAGACCACACTGAGCCTCC CCTAGGAGCACGTCTTGCTACCCTCTTTTGAAGATGTTCTCTAGCCTTCCACCAGCAGCGAGGAATTAACCCTGTGTCCTCAGTCGCCAGCACTTACAGC TCCAACTTTTTTGAATGACCATCTGGTTGAAAAATCTTTCTCATATAAGTTTAACCACACTTTGATTTGGGTTCATTTTTTGTTTTGTTTTTTTCAATCA TGATATTCAGAAAAATCCAGGATCCAAAATGTGGCGTTTTTCTAAGAATGAAAATTATATGTAAGCTTTTAAGCATCATGAAGAACAATTTATGTTCACA TTAAGATACGTTCTAAAGGGGGATGGCCAAGGGGTGACATCTTAATTCCTAAACTACCTTAGCTGCATAGTGGAAGAGGAGAGCATGAAGCAAAGAATTC CAGGAAACCCAAGAGGCTGAGAATTCTTTTGTCTACCATAGAATTATTATCCAGACTGGAATTTTTGTTTGTTAGAACACCCTTCAGTTGCAATATGCTA ATCCCACTTTACAAAGAATATAAAAGCTATATTTTGAAGACTTGAGTTATTTCAGAAAAAACTACAGCCCTTTTTGTCTTACCTGCCTTTTACTTTCGTG TGGATATGTGAAGCATTGGGTCGGGAACTAGCTGTAGAACACAACTAAAAACTCATGTCTTTTTTCACAGAATAATGTGCCAGTTTTTTGTAGCAATGTT ATTTCTCTTGGAAGCAGAAATGCTTTGTACCAGAGCACCTCCAAACTGCATTGAGGAGAAGTTCCAGAACCATCCCCTTTTTCCATTTTTATATAATTTA TAAAGAAAGATTAAAGCCATGTTGACTATTTTACAGCCACTGGAGTTAACTAACCCTTCCTTGTATCTGTCTTCCCAGGAGAGAATGAAGCAAAACAGGA ATTTGGTTTTCTTTTGATGTCCAGTTACACCATCCATTCTGTTAATTTTGAAAAAATATACCCTCCCTTTAGTTTGTTGGGGGATATAAATTATTCTCAG GAAGAATATAATGAACTGTACAGTTACTTTGACCTATTAAAAAGGTGTTACCAGTAAAGTTCTTGTTGTAATATCCTTTCTTTTGGTTCTGTTTCTTCAG ATGGCTTTTCAGGTGACTTGTCAGATAAGATAACATACAAGAGAGTTCCCAATAATTATCAGGAAGCCTCTCACTAATTAGTTTCTTTTTTTTTATTTAA TCTTCTAAACAAGTCAAGGATGTTACCACAGAAGGGTAGCGTGGCGTAAGTTAACCTTTTCATTCCATCGTCACCTATTAAATGCAGAGCTCAGGATGGA AGTAGCAATGGTCTTCAATGAAAGAACCTTCCCCTGCGCAGGGTCTCACTATTCCGCTAACTCGGACTCAAAGCATAATTCACTTGTCAAGGAAATTGTT AGCATTTCCCAGGCCCACCCTAGTCTTGCTAGAATCTCTGTGAGCCACAGAGCAGGGAGCAAATAGAACTGAGATCTCCAGCACTAGGGACTCAGACTCC AAAGGGAGGGATTTGAGTGAATTTTTTCCAGGTAAATACATGCTCTTATGTGC

PTK2.au.8.f (forward primer)
PTK2.u.r.53 (reverse primer)
Single exon skipping

Frame 0

SRTTVSPRREAWEEARLLSPSAAPSSSAFASRR RAAVRAELGLPCIFQLQIQCLLQFPQSSSRITEG RI*QIPSI*QNNGSCLP*PQLESHTKFEY*DSP GYWYGTFSWCNGASIKGLSLF*KQ**ANHLGQY YQAWRCY*CQGHHSEDSGQSQSKACGLLWIPPQS PAVRGGSLASRGYGRLQCEGEV*ACSPTRGVEI *IENSLFAKRISKPVY*R*ANFEFLLSTGEERL YVRDS*SSGPGNCFEVGLSRNTAIILGDAGQCTR KEV*L*SIRKRCWFKAIFS*EFTGFCQGQNTKK TDPTNI*TICQP**RRKYSEIL*DPVSSLQI** GMLQVCSWFKLDYFSGTGNRPRRRNQLPNGQGLQ SHTSC*LHSSANHSVFKQ*RQGQKRNATTKNSR CTRASDSDGTIPNHCGEYG*PNRWVLPAGEWNL AVIYHQTSERR*TGFAINTKVGQQRKARHADTRR LCVRNR*LC*DYR*RRYLHHALNQGL*DSKRKN RTWTMYWRRPIWRCTSRHLYESRESSFGGCN*N M*KLYFGQREREISSRSLNNASV*PSSYCEADWS HHRESCLDNHGAVHTWRAEVIFASKEIQFGSSI FDPVCLSA*YSSCISREQKICTQGHCCSECSGV LK*LCKIRRLWIIPIYGR*YLLQSFQRKIAY*MD GSRVNQFSTFYLS**RMDVWCVYVGDTDAWCEA FSRSEEQ*CNRSN*KWGKITNASKLSSYPLQPY DEMLGL*PQQAAQVY*T*SSAQHNPGGREGSARR AHEDGVQKTGHSVLGLRRV**STAQAQQTGLSQ SEVQRRILSQPTAHGTNQSLPGFWLPWFTWNHS HGWQHLSRSGISFGPNRFMES*TSGDSNVAAQCG GLYSIGPARDWASVANPSDGRASNPTATGNGRR SALAGKRGKISET*CETLSRQY*QGGWKSSGSD WKPTYISACG*TRSCSSTKETASPWSSRSSGKPC QPQQPC*QLQRGCQASAPGNQPPSYCQPGPVE* *GVRECDGPGESCHRDVQ*NPASPTRGVCPYGE GSRLGPEDIIGHCG*DHSPPTSQHPPRD*DGTEA IEL*PG*AHQQDETGPAVCHDQPPARVQKANAD CCSRPGCGCQKLTRCH*PSKTENAWADETTLSL P*EHVLLPSFEDVL*PSTSSEELTLCPQSPALTA PTFLNDHLVEKSFSYKFNHTLIWVHFLFCFFQS *YSEKSRIQNVAFF*E*KLYVSF*AS*RTIYVH IKIRSKGGWPRGDILIPKLP*LHSGRGEHEAKNS RKPKRLRILLSTIELLSRLEFLFVRTPFSCNML IPLYKEYKSYILKT*VISEKTTALFVLPAFYFR VDM*SIGSGTSCRTQLKTHVFFHRIMCQFFVAML FLLEAEMLCTRAPPNCIEEKFQNHPLFPFLYNL *RKIKAMLTILQPLELTNPSLYLSSQERMKQNR NLVFF*CPVTPSILLILKKYTLPLVCWGI*IILR KNIMNCTVTLTY*KGVTSKVLVVISFLLVLFLQ MAFQVTCQIR*HTREFPIIIRKPLTN*FLFFYL IF*TSQGCYHRRVAWRKLTFSFHRHLLNAELRME VAMVFNERTFPCAGSHYSANSDSKHNSLVKEIV SISQAHPSLARISVSHRAGSK*N*DLQH*GLRL QREGFE*IFSR*IHALMC

Frame 1

PGPL*ARGVRRGRKRGCCRPAPPRRRLPSLHGA EPRSEQNWGSLASSSYKFSAFCSFPRAPQE*RK GEYDRYLASSKIMAAAYLDPNLNHTPNSSTKTHL GTGMERSPGAMERVLKVFHYFESNSEPTTWASI IRHGDATDVRGIIQKIVDSHKVKHVACYGFRLS HLRSEEVHWLHVDMGVSSVREKYELAHPPEEWKY ELRIRYLPKGFLNQFTEDKPTLNFFYQQVKSDY MLEIADQVDQEIALKLGCLEIRRSYWEMRGNAL EKKSNYEVLEKDVGLKRFFPKSLLDSVKAKTLRK LIQQTFRQFANLNREESILKFFEILSPVYRFDK ECFKCALGSSWIISVELAIGPEEGISYLTDKGC NPTHLADFTQVQTIQYSNSEDKDRKGMLQLKIAG APEPLTVTAPSLTIAENMADLIDGYCRLVNGTS QSFIIRPQKEGERALPSIPKLANSEKQGMRTHA VSVSETDDYAEIIDEEDTYTMPSTRDYEIQRERI ELGRCIGEGQFGDVHQGIYMSPENPALAVAIKT CKNCTSDSVREKFLQEALTMRQFDHPHIVKLIG VITENPVWIIMELCTLGELRSFLQVRKYSLDLAS LILYAYQLSTALAYLESKRFVHRDIAARNVLVS SNDCVKLGDFGLSRYMEDSTYYKASKGKLPIKW MAPESINFRRFTSASDVWMFGVCMWEILMHGVKP FQGVKNNDVIGRIENGERLPMPPNCPPTLYSLM TKCWAYDPSRRPRFTELKAQLSTILEEEKAQQE ERMRMESRRQATVSWDSGGSDEAPPKPSRPGYPS PRSSEGFYPSPQHMVQTNHYQVSGYPGSHGITA MAGSIYPGQASLLDQTDSWNHRPQEIAMWQPNV EDSTVLDLRGIGQVLPTHLMEERLIRQQQEMEED QRWLEKEERFLKPDVRLSRGSIDREDGSLQGPI GNQHIYQPVGKPDPAAPPKKPPRPGAPGHLGSL ASLSSPADSYNEGVKLQPQEISPPPTANLDRSND KVYENVTGLVKAVIEMSSKIQPAPPEEYVPMVK EVGLALRTLLATVDETIPLLPASTHREIEMAQK LLNSDLGELINKMKLAQQYVMTSLQQEYKKQMLT AAHALAVDAKNLLDVIDQARLKMLGQTRPH*AS PRSTSCYPLLKMFSSLPPAARN*PCVLSRQHLQ LQLF*MTIWLKNLSHISLTTL*FGFIFCFVFFNH DIQKNPGSKMWRFSKNENYM*AFKHHEEQFMFT LRYVLKGDGQGVTS*FLNYLSCIVEEESMKQRI PGNPRG*EFFCLP*NYYPDWNFCLLEHPSVAIC* SHFTKNIKAIF*RLELFQKKLQPFLSYLPFTFV WICEALGRELAVEHN*KLMSFFTE*CASFL*QC YFSWKQKCFVPEHLQTALRRSSRTIPFFHFYIIY KERLKPC*LFYSHWS*LTLPCICLPRRE*SKTG IWFSFDVQLHHPFC*F*KNIPSL*FVGGYKLFS GRI**TVQLL*PIKKVLPVKFLL*YPFFWFCFFR WLFR*LVR*DNIQESSQ*LSGSLSLISFFFFI* SSKQVKDVTTEG*RGVS*PFHSIVTY*MQSSGW K*QWSSMKEPSPAQGLTIPLTRTQSIIHLSRKLL AFPRPTLVLLESL*ATEQGANRTEISSTRDSDS KGRDLSEFFPGKYMLLC

Frame 2

PDHCEPAA*GVGGSAAAVAQRRPVVVCLRFTAP SRGPSRTGAPLHLPVTNSVPSAVSPELLKNNGR ENMTDT*HLAK*WQLLTLTPT*ITHQIRVLRLT WVLVWNVLLVQWSEY*RSFIILKAIVSQPPGPVL SGMEMLLMSGASFRR*WTVTK*SMWPAMDSASV TCGQRRFTGFTWIWASPV*GRSMSLLTHQRSGN MN*EFVICQKDF*TSLLKISQL*ISSINR*RAII C*R*LIKWTRKLL*SWVV*KYGDHTGRCGAMH* KRSLTMKY*KKMLV*SDFFLRVYWILSRPKH*E N*SNKHLDNLPTLIEKKVF*NSLRSCLQSTDLIR NASSVLLVQAGLFQWNWQSAQKKESVT*RTRAA IPHILLTSLKCKPFSIQTVKTRTEKECYN*K*Q VHPSL*Q*RHHP*PLRRIWLT**MGTAGW*MEPR SHLSSDLRKKVNGLCHQYQSWPTAKSKACGHTP SLCQKQMIMLRL*MKKILTPCPQPGIMRFKEKE *NLDDVLEKANLEMYIKAFI*VQRIQLWRLQLKH VKTVLRTA*ERNFFKKP*QCVSLTILIL*S*LE SSQRILSG*SWSCAHLES*GHFCK*GNTVWI*H L*SCMPISLVQLLHI*RAKDLYTGTLLLGMFWCP QMIV*N*ETLDYPDIWKIVLTTKLPKENCLLNG WLQSQSIFDVLPQLVTYGCLVCVCGRY*CMV*S LFKE*RTMM*SVELKMGKDYQCLQIVLLPSTAL* RNAGPMTPAGGPGLLNLKLSSAQSWRKRRLSKK SA*GWSPEDRPQCPGTPEGLMKHRPSPADRVIP VRGPAKDFIPAHSTWYKPIITRFLATLVHMESQP WLAASIQVRHLFWTKQIHGIIDLRR*QCGSPMW RTLQYWTCEGLGKCCQPI*WKSV*SDSNRKWKK ISAGWKKRKDF*NLM*DSLEAVLTGRMEVFRVRL ETNIYISLWVNQILQLHQRNRLALELPVIWEAL PASAALLTATTRVSSFSPRKSAPLLLPTWTGRM IRCTRM*RAW*KLSSRCPVKSSQPHQRSMSLW*R KSAWP*GHYWPLWMRPFPSYQPAPTERLRWHRS Y*TLTWVSSSTR*NWPSSMS*PASSKSTKSKC* LLLTPWLWMPKTYSMSLTKQD*KCLGRRDHTEPP LGARLATLF*RCSLAFHQQRGINPVSSVASTYS SNFFE*PSG*KIFLI*V*PHFDLGSFFVLFFSI MIFRKIQDPKCGVFLRMKIICKLLSIMKNNLCSH *DTF*RGMAKG*HLNS*TTLAA*WKRRA*SKEF QETQEAENSFVYHRIIIQTGIFVC*NTLQLQYA NPTLQRI*KLYFEDLSYFRKNYSPFCLTCLLLSC GYVKHWVGN*L*NTTKNSCLFSQNNVPVFCSNV ISLGSRNALYQSTSKLH*GEVPEPSPFSIFI*F IKKD*SHVDYFTATGVN*PFLVSVFPGENEAKQE FGFLLMSSYTIHSVNFEKIYPPFSLLGDINYSQ EEYNELYSYFDLLKRCYQ*SSCCNILSFGSVSS DGFSGDLSDKITYKRVPNNYQEASH*LVSFFLFN LLNKSRMLPQKGSVA*VNLFIPSSPIKCRAQDG SSNGLQ*KNLPLRRVSLFR*LGLKA*FTCQGNC *HFPGPP*SC*NLCEPQSREQIELRSPALGTQTP KGGI*VNFFQVNTCSYV

Sequence PTK2.bAug05

TCCCGGACCACTGTGAGCCCGCGGCGTGAGGCGTGGGAGGAAGCGCGGCTGCTGTCGCCCAGCGCCGCCCCGTCGTCGTCTGCCTTCGCTTCACGGCGCC GAGCCGCGGTCCGAAGTCTTGCTGTGTCGCCCAGGCTGCCAGGCTGGAGTGCAATGGCATCATCTCGGCTCACTGCAACCTCTGCCTCCAATATGACAGA TACCTAGCATCTAGCAAAATAATGGCAGCTGCTTACCTTGACCCCAACTTGAATCACACACCAAATTCGAGTACTAAGACTCACCTGGGTACTGGTATGG AACGTTCTCCTGGTGCAATGGAGCGAGTATTAAAGGTCTTTCATTATTTTGAAAGCAATAGTGAGCCAACCACCTGGGCCAGTATTATCAGGCATGGAGA TGCTACTGATGTCAGGGGCATCATTCAGAAGATAGTGGACAGTCACAAAGTAAAGCATGTGGCCTGCTATGGATTCCGCCTCAGTCACCTGCGGTCAGAG GAGGTTCACTGGCTTCACGTGGATATGGGCGTCTCCAGTGTGAGGGAGAAGTATGAGCTTGCTCACCCACCAGAGGAGTGGAAATATGAATTGAGAATTC GTTATTTGCCAAAAGGATTTCTAAACCAGTTTACTGAAGATAAGCCAACTTTGAATTTCTTCTATCAACAGGTGAAGAGCGATTATATGTTAGAGATAGC TGATCAAGTGGACCAGGAAATTGCTTTGAAGTTGGGTTGTCTAGAAATACGGCGATCATACTGGGAGATGCGGGGCAATGCACTAGAAAAGAAGTCTAAC TATGAAGTATTAGAAAAAGATGTTGGTTTAAAGCGATTTTTTCCTAAGAGTTTACTGGATTCTGTCAAGGCCAAAACACTAAGAAAACTGATCCAACAAA CATTTAGACAATTTGCCAACCTTAATAGAGAAGAAAGTATTCTGAAATTCTTTGAGATCCTGTCTCCAGTCTACAGATTTGATAAGGAATGCTTCAAGTG TGCTCTTGGTTCAAGCTGGATTATTTCAGTGGAACTGGCAATCGGCCCAGAAGAAGGAATCAGTTACCTAACGGACAAGGGCTGCAATCCCACACATCTT GCTGACTTCACTCAAGTGCAAACCATTCAGTATTCAAACAGTGAAGACAAGGACAGAAAAGGAATGCTACAACTAAAAATAGCAGGTGCACCCGAGCCTC TGACAGTGACGGCACCATCCCTAACCATTGCGGAGAATATGGCTGACCTAATAGATGGGTACTGCCGGCTGGTGAATGGAACCTCGCAGTCATTTATCAT CAGACCTCAGAAAGAAGGTGAACGGGCTTTGCCATCAATACCAAAGTTGGCCAACAGCGAAAAGCAAGGCATGCGGACACACGCCGTCTCTGTGTCAGAA ACAGATGATTATGCTGAGATTATAGATGAAGAAGATACTTACACCATGCCCTCAACCAGGGATTATGAGATTCAAAGAGAAAGAATAGAACTTGGACGAT GTATTGGAGAAGGCCAATTTGGAGATGTACATCAAGGCATTTATATGAGTCCAGAGAATCCAGCTTTGGCGGTTGCAATTAAAACATGTAAAAACTGTAC TTCGGACAGCGTGAGAGAGAAATTTCTTCAAGAAGCCTTAACAATGCGTCAGTTTGACCATCCTCATATTGTGAAGCTGATTGGAGTCATCACAGAGAAT CCTGTCTGGATAATCATGGAGCTGTGCACACTTGGAGAGCTGAGGTCATTTTTGCAAGTAAGGAAATACAGTTTGGATCTAGCATCTTTGATCCTGTATG CCTATCAGCTTAGTACAGCTCTTGCATATCTAGAGAGCAAAAGATTTGTACACAGGGACATTGCTGCTCGGAATGTTCTGGTGTCCTCAAATGATTGTGT AAAATTAGGAGACTTTGGATTATCCCGATATATGGAAGATAGTACTTACTACAAAGCTTCCAAAGGAAAATTGCCTATTAAATGGATGGCTCCAGAGTCA ATCAATTTTCGACGTTTTACCTCAGCTAGTGACGTATGGATGTTTGGTGTGTGTATGTGGGAGATACTGATGCATGGTGTGAAGCCTTTTCAAGGAGTGA AGAACAATGATGTAATCGGTCGAATTGAAAATGGGGAAAGATTACCAATGCCTCCAAATTGTCCTCCTACCCTCTACAGCCTTATGACGAAATGCTGGGC CTATGACCCCAGCAGGCGGCCCAGGTTTACTGAACTTAAAGCTCAGCTCAGCACAATCCTGGAGGAAGAGAAGGCTCAGCAAGAAGAGCGCATGAGGATG GAGTCCAGAAGACAGGCCACAGTGTCCTGGGACTCCGGAGGGTCTGATGAAGCACCGCCCAAGCCCAGCAGACCGGGTTATCCCAGTCCGAGGTCCAGCG AAGGATTTTATCCCAGCCCACAGCACATGGTACAAACCAATCATTACCAGGTTTCTGGCTACCCTGGTTCACATGGAATCACAGCCATGGCTGGCAGCAT CTATCCAGGTCAGGCATCTCTTTTGGACCAAACAGATTCATGGAATCATAGACCTCAGGAGATAGCAATGTGGCAGCCCAATGTGGAGGACTCTACAGTA TTGGACCTGCGAGGGATTGGGCAAGTGTTGCCAACCCATCTGATGGAAGAGCGTCTAATCCGACAGCAACAGGAAATGGAAGAAGATCAGCGCTGGCTGG AAAAAGAGGAAAGATTTCTGAAACCTGATGTGAGACTCTCTCGAGGCAGTATTGACAGGGAGGATGGAAGTCTTCAGGGTCCGATTGGAAACCAACATAT ATATCAGCCTGTGGGTAAACCAGATCCTGCAGCTCCACCAAAGAAACCGCCTCGCCCTGGAGCTCCCGGTCATCTGGGAAGCCTTGCCAGCCTCAGCAGC CCTGCTGACAGCTACAACGAGGGTGTCAAGCTTCAGCCCCAGGAAATCAGCCCCCCTCCTACTGCCAACCTGGACCGGTCGAATGATAAGGTGTACGAGA ATGTGACGGGCCTGGTGAAAGCTGTCATCGAGATGTCCAGTAAAATCCAGCCAGCCCCACCAGAGGAGTATGTCCCTATGGTGAAGGAAGTCGGCTTGGC CCTGAGGACATTATTGGCCACTGTGGATGAGACCATTCCCCTCCTACCAGCCAGCACCCACCGAGAGATTGAGATGGCACAGAAGCTATTGAACTCTGAC CTGGGTGAGCTCATCAACAAGATGAAACTGGCCCAGCAGTATGTCATGACCAGCCTCCAGCAAGAGTACAAAAAGCAAATGCTGACTGCTGCTCACGCCC TGGCTGTGGATGCCAAAAACTTACTCGATGTCATTGACCAAGCAAGACTGAAAATGCTTGGGCAGACGAGACCACACTGAGCCTCCCCTAGGAGCACGTC TTGCTACCCTCTTTTGAAGATGTTCTCTAGCCTTCCACCAGCAGCGAGGAATTAACCCTGTGTCCTCAGTCGCCAGCACTTACAGCTCCAACTTTTTTGA ATGACCATCTGGTTGAAAAATCTTTCTCATATAAGTTTAACCACACTTTGATTTGGGTTCATTTTTTGTTTTGTTTTTTTCAATCATGATATTCAGAAAA ATCCAGGATCCAAAATGTGGCGTTTTTCTAAGAATGAAAATTATATGTAAGCTTTTAAGCATCATGAAGAACAATTTATGTTCACATTAAGATACGTTCT AAAGGGGGATGGCCAAGGGGTGACATCTTAATTCCTAAACTACCTTAGCTGCATAGTGGAAGAGGAGAGCATGAAGCAAAGAATTCCAGGAAACCCAAGA GGCTGAGAATTCTTTTGTCTACCATAGAATTATTATCCAGACTGGAATTTTTGTTTGTTAGAACACCCTTCAGTTGCAATATGCTAATCCCACTTTACAA AGAATATAAAAGCTATATTTTGAAGACTTGAGTTATTTCAGAAAAAACTACAGCCCTTTTTGTCTTACCTGCCTTTTACTTTCGTGTGGATATGTGAAGC ATTGGGTCGGGAACTAGCTGTAGAACACAACTAAAAACTCATGTCTTTTTTCACAGAATAATGTGCCAGTTTTTTGTAGCAATGTTATTTCTCTTGGAAG CAGAAATGCTTTGTACCAGAGCACCTCCAAACTGCATTGAGGAGAAGTTCCAGAACCATCCCCTTTTTCCATTTTTATATAATTTATAAAGAAAGATTAA AGCCATGTTGACTATTTTACAGCCACTGGAGTTAACTAACCCTTCCTTGTATCTGTCTTCCCAGGAGAGAATGAAGCAAAACAGGAATTTGGTTTTCTTT TGATGTCCAGTTACACCATCCATTCTGTTAATTTTGAAAAAATATACCCTCCCTTTAGTTTGTTGGGGGATATAAATTATTCTCAGGAAGAATATAATGA ACTGTACAGTTACTTTGACCTATTAAAAAGGTGTTACCAGTAAAGTTCTTGTTGTAATATCCTTTCTTTTGGTTCTGTTTCTTCAGATGGCTTTTCAGGT GACTTGTCAGATAAGATAACATACAAGAGAGTTCCCAATAATTATCAGGAAGCCTCTCACTAATTAGTTTCTTTTTTTTTATTTAATCTTCTAAACAAGT CAAGGATGTTACCACAGAAGGGTAGCGTGGCGTAAGTTAACCTTTTCATTCCATCGTCACCTATTAAATGCAGAGCTCAGGATGGAAGTAGCAATGGTCT TCAATGAAAGAACCTTCCCCTGCGCAGGGTCTCACTATTCCGCTAACTCGGACTCAAAGCATAATTCACTTGTCAAGGAAATTGTTAGCATTTCCCAGGC CCACCCTAGTCTTGCTAGAATCTCTGTGAGCCACAGAGCAGGGAGCAAATAGAACTGAGATCTCCAGCACTAGGGACTCAGACTCCAAAGGGAGGGATTT GAGTGAATTTTTTCCAGGTAAATACATGCTCTTATGTGC

PTK2.au.8.f (forward primer)
PTK2.u.r.53 (reverse primer)
Single exon skipping

Frame 0

SRTTVSPRREAWEEARLLSPSAAPSSSAFASRR RAAVRSLAVSPRLPGWSAMASSRLTATSASNMTD T*HLAK*WQLLTLTPT*ITHQIRVLRLTWVLVW NVLLVQWSEY*RSFIILKAIVSQPPGPVLSGME MLLMSGASFRR*WTVTK*SMWPAMDSASVTCGQR RFTGFTWIWASPV*GRSMSLLTHQRSGNMN*EF VICQKDF*TSLLKISQL*ISSINR*RAIIC*R* LIKWTRKLL*SWVV*KYGDHTGRCGAMH*KRSLT MKY*KKMLV*SDFFLRVYWILSRPKH*EN*SNK HLDNLPTLIEKKVF*NSLRSCLQSTDLIRNASS VLLVQAGLFQWNWQSAQKKESVT*RTRAAIPHIL LTSLKCKPFSIQTVKTRTEKECYN*K*QVHPSL *Q*RHHP*PLRRIWLT**MGTAGW*MEPRSHLS SDLRKKVNGLCHQYQSWPTAKSKACGHTPSLCQK QMIMLRL*MKKILTPCPQPGIMRFKEKE*NLDD VLEKANLEMYIKAFI*VQRIQLWRLQLKHVKTV LRTA*ERNFFKKP*QCVSLTILIL*S*LESSQRI LSG*SWSCAHLES*GHFCK*GNTVWI*HL*SCM PISLVQLLHI*RAKDLYTGTLLLGMFWCPQMIV *N*ETLDYPDIWKIVLTTKLPKENCLLNGWLQSQ SIFDVLPQLVTYGCLVCVCGRY*CMV*SLFKE* RTMM*SVELKMGKDYQCLQIVLLPSTAL*RNAG PMTPAGGPGLLNLKLSSAQSWRKRRLSKKSA*GW SPEDRPQCPGTPEGLMKHRPSPADRVIPVRGPA KDFIPAHSTWYKPIITRFLATLVHMESQPWLAA SIQVRHLFWTKQIHGIIDLRR*QCGSPMWRTLQY WTCEGLGKCCQPI*WKSV*SDSNRKWKKISAGW KKRKDF*NLM*DSLEAVLTGRMEVFRVRLETNI YISLWVNQILQLHQRNRLALELPVIWEALPASAA LLTATTRVSSFSPRKSAPLLLPTWTGRMIRCTR M*RAW*KLSSRCPVKSSQPHQRSMSLW*RKSAW P*GHYWPLWMRPFPSYQPAPTERLRWHRSY*TLT WVSSSTR*NWPSSMS*PASSKSTKSKC*LLLTP WLWMPKTYSMSLTKQD*KCLGRRDHTEPPLGAR LATLF*RCSLAFHQQRGINPVSSVASTYSSNFFE *PSG*KIFLI*V*PHFDLGSFFVLFFSIMIFRK IQDPKCGVFLRMKIICKLLSIMKNNLCSH*DTF *RGMAKG*HLNS*TTLAA*WKRRA*SKEFQETQE AENSFVYHRIIIQTGIFVC*NTLQLQYANPTLQ RI*KLYFEDLSYFRKNYSPFCLTCLLLSCGYVK HWVGN*L*NTTKNSCLFSQNNVPVFCSNVISLGS RNALYQSTSKLH*GEVPEPSPFSIFI*FIKKD* SHVDYFTATGVN*PFLVSVFPGENEAKQEFGFL LMSSYTIHSVNFEKIYPPFSLLGDINYSQEEYNE LYSYFDLLKRCYQ*SSCCNILSFGSVSSDGFSG DLSDKITYKRVPNNYQEASH*LVSFFLFNLLNK SRMLPQKGSVA*VNLFIPSSPIKCRAQDGSSNGL Q*KNLPLRRVSLFR*LGLKA*FTCQGNC*HFPG PP*SC*NLCEPQSREQIELRSPALGTQTPKGGI *VNFFQVNTCSYV

Frame 1

PGPL*ARGVRRGRKRGCCRPAPPRRRLPSLHGA EPRSEVLLCRPGCQAGVQWHHLGSLQPLPPI*Q IPSI*QNNGSCLP*PQLESHTKFEY*DSPGYWYG TFSWCNGASIKGLSLF*KQ**ANHLGQYYQAWR CY*CQGHHSEDSGQSQSKACGLLWIPPQSPAVR GGSLASRGYGRLQCEGEV*ACSPTRGVEI*IENS LFAKRISKPVY*R*ANFEFLLSTGEERLYVRDS *SSGPGNCFEVGLSRNTAIILGDAGQCTRKEV* L*SIRKRCWFKAIFS*EFTGFCQGQNTKKTDPTN I*TICQP**RRKYSEIL*DPVSSLQI**GMLQV CSWFKLDYFSGTGNRPRRRNQLPNGQGLQSHTS C*LHSSANHSVFKQ*RQGQKRNATTKNSRCTRAS DSDGTIPNHCGEYG*PNRWVLPAGEWNLAVIYH QTSERR*TGFAINTKVGQQRKARHADTRRLCVR NR*LC*DYR*RRYLHHALNQGL*DSKRKNRTWTM YWRRPIWRCTSRHLYESRESSFGGCN*NM*KLY FGQREREISSRSLNNASV*PSSYCEADWSHHRE SCLDNHGAVHTWRAEVIFASKEIQFGSSIFDPVC LSA*YSSCISREQKICTQGHCCSECSGVLK*LC KIRRLWIIPIYGR*YLLQSFQRKIAY*MDGSRV NQFSTFYLS**RMDVWCVYVGDTDAWCEAFSRSE EQ*CNRSN*KWGKITNASKLSSYPLQPYDEMLG L*PQQAAQVY*T*SSAQHNPGGREGSARRAHED GVQKTGHSVLGLRRV**STAQAQQTGLSQSEVQR RILSQPTAHGTNQSLPGFWLPWFTWNHSHGWQH LSRSGISFGPNRFMES*TSGDSNVAAQCGGLYS IGPARDWASVANPSDGRASNPTATGNGRRSALAG KRGKISET*CETLSRQY*QGGWKSSGSDWKPTY ISACG*TRSCSSTKETASPWSSRSSGKPCQPQQ PC*QLQRGCQASAPGNQPPSYCQPGPVE**GVRE CDGPGESCHRDVQ*NPASPTRGVCPYGEGSRLG PEDIIGHCG*DHSPPTSQHPPRD*DGTEAIEL* PG*AHQQDETGPAVCHDQPPARVQKANADCCSRP GCGCQKLTRCH*PSKTENAWADETTLSLP*EHV LLPSFEDVL*PSTSSEELTLCPQSPALTAPTFL NDHLVEKSFSYKFNHTLIWVHFLFCFFQS*YSEK SRIQNVAFF*E*KLYVSF*AS*RTIYVHIKIRS KGGWPRGDILIPKLP*LHSGRGEHEAKNSRKPK RLRILLSTIELLSRLEFLFVRTPFSCNMLIPLYK EYKSYILKT*VISEKTTALFVLPAFYFRVDM*S IGSGTSCRTQLKTHVFFHRIMCQFFVAMLFLLE AEMLCTRAPPNCIEEKFQNHPLFPFLYNL*RKIK AMLTILQPLELTNPSLYLSSQERMKQNRNLVFF *CPVTPSILLILKKYTLPLVCWGI*IILRKNIM NCTVTLTY*KGVTSKVLVVISFLLVLFLQMAFQV TCQIR*HTREFPIIIRKPLTN*FLFFYLIF*TS QGCYHRRVAWRKLTFSFHRHLLNAELRMEVAMV FNERTFPCAGSHYSANSDSKHNSLVKEIVSISQA HPSLARISVSHRAGSK*N*DLQH*GLRLQREGF E*IFSR*IHALMC

Frame 2

PDHCEPAA*GVGGSAAAVAQRRPVVVCLRFTAP SRGPKSCCVAQAARLECNGIISAHCNLCLQYDR YLASSKIMAAAYLDPNLNHTPNSSTKTHLGTGM ERSPGAMERVLKVFHYFESNSEPTTWASIIRHGD ATDVRGIIQKIVDSHKVKHVACYGFRLSHLRSE EVHWLHVDMGVSSVREKYELAHPPEEWKYELRI RYLPKGFLNQFTEDKPTLNFFYQQVKSDYMLEIA DQVDQEIALKLGCLEIRRSYWEMRGNALEKKSN YEVLEKDVGLKRFFPKSLLDSVKAKTLRKLIQQ TFRQFANLNREESILKFFEILSPVYRFDKECFKC ALGSSWIISVELAIGPEEGISYLTDKGCNPTHL ADFTQVQTIQYSNSEDKDRKGMLQLKIAGAPEP LTVTAPSLTIAENMADLIDGYCRLVNGTSQSFII RPQKEGERALPSIPKLANSEKQGMRTHAVSVSE TDDYAEIIDEEDTYTMPSTRDYEIQRERIELGR CIGEGQFGDVHQGIYMSPENPALAVAIKTCKNCT SDSVREKFLQEALTMRQFDHPHIVKLIGVITEN PVWIIMELCTLGELRSFLQVRKYSLDLASLILY AYQLSTALAYLESKRFVHRDIAARNVLVSSNDCV KLGDFGLSRYMEDSTYYKASKGKLPIKWMAPES INFRRFTSASDVWMFGVCMWEILMHGVKPFQGV KNNDVIGRIENGERLPMPPNCPPTLYSLMTKCWA YDPSRRPRFTELKAQLSTILEEEKAQQEERMRM ESRRQATVSWDSGGSDEAPPKPSRPGYPSPRSS EGFYPSPQHMVQTNHYQVSGYPGSHGITAMAGSI YPGQASLLDQTDSWNHRPQEIAMWQPNVEDSTV LDLRGIGQVLPTHLMEERLIRQQQEMEEDQRWL EKEERFLKPDVRLSRGSIDREDGSLQGPIGNQHI YQPVGKPDPAAPPKKPPRPGAPGHLGSLASLSS PADSYNEGVKLQPQEISPPPTANLDRSNDKVYE NVTGLVKAVIEMSSKIQPAPPEEYVPMVKEVGLA LRTLLATVDETIPLLPASTHREIEMAQKLLNSD LGELINKMKLAQQYVMTSLQQEYKKQMLTAAHA LAVDAKNLLDVIDQARLKMLGQTRPH*ASPRSTS CYPLLKMFSSLPPAARN*PCVLSRQHLQLQLF* MTIWLKNLSHISLTTL*FGFIFCFVFFNHDIQK NPGSKMWRFSKNENYM*AFKHHEEQFMFTLRYVL KGDGQGVTS*FLNYLSCIVEEESMKQRIPGNPR G*EFFCLP*NYYPDWNFCLLEHPSVAIC*SHFT KNIKAIF*RLELFQKKLQPFLSYLPFTFVWICEA LGRELAVEHN*KLMSFFTE*CASFL*QCYFSWK QKCFVPEHLQTALRRSSRTIPFFHFYIIYKERL KPC*LFYSHWS*LTLPCICLPRRE*SKTGIWFSF DVQLHHPFC*F*KNIPSL*FVGGYKLFSGRI** TVQLL*PIKKVLPVKFLL*YPFFWFCFFRWLFR *LVR*DNIQESSQ*LSGSLSLISFFFFI*SSKQV KDVTTEG*RGVS*PFHSIVTY*MQSSGWK*QWS SMKEPSPAQGLTIPLTRTQSIIHLSRKLLAFPR PTLVLLESL*ATEQGANRTEISSTRDSDSKGRDL SEFFPGKYMLLC

Sequence PTK2.aAug05

TCCCGGACCACTGTGAGCCCGCGGCGTGAGGCGTGGGAGGAAGCGCGGCTGCTGTCGCCCAGCGCCGCCCCGTCGTCGTCTGCCTTCGCTTCACGGCGCC GAGCCGCGGTCCGAAGTCTTGCTGTGTCACCCAGGCTGCCAGGCTGGAGTGGAGTGGCATGATCTCGGCTGACTGCAACCTCTGCCTCCCAGAATATGAC AGATACCTAGCATCTAGCAAAATAATGGCAGCTGCTTACCTTGACCCCAACTTGAATCACACACCAAATTCGAGTACTAAGACTCACCTGGGTACTGGTA TGGAACGTTCTCCTGGTGCAATGGAGCGAGTATTAAAGGTCTTTCATTATTTTGAAAGCAATAGTGAGCCAACCACCTGGGCCAGTATTATCAGGCATGG AGATGCTACTGATGTCAGGGGCATCATTCAGAAGATAGTGGACAGTCACAAAGTAAAGCATGTGGCCTGCTATGGATTCCGCCTCAGTCACCTGCGGTCA GAGGAGGTTCACTGGCTTCACGTGGATATGGGCGTCTCCAGTGTGAGGGAGAAGTATGAGCTTGCTCACCCACCAGAGGAGTGGAAATATGAATTGAGAA TTCGTTATTTGCCAAAAGGATTTCTAAACCAGTTTACTGAAGATAAGCCAACTTTGAATTTCTTCTATCAACAGGTGAAGAGCGATTATATGTTAGAGAT AGCTGATCAAGTGGACCAGGAAATTGCTTTGAAGTTGGGTTGTCTAGAAATACGGCGATCATACTGGGAGATGCGGGGCAATGCACTAGAAAAGAAGTCT AACTATGAAGTATTAGAAAAAGATGTTGGTTTAAAGCGATTTTTTCCTAAGAGTTTACTGGATTCTGTCAAGGCCAAAACACTAAGAAAACTGATCCAAC AAACATTTAGACAATTTGCCAACCTTAATAGAGAAGAAAGTATTCTGAAATTCTTTGAGATCCTGTCTCCAGTCTACAGATTTGATAAGGAATGCTTCAA GTGTGCTCTTGGTTCAAGCTGGATTATTTCAGTGGAACTGGCAATCGGCCCAGAAGAAGGAATCAGTTACCTAACGGACAAGGGCTGCAATCCCACACAT CTTGCTGACTTCACTCAAGTGCAAACCATTCAGTATTCAAACAGTGAAGACAAGGACAGAAAAGGAATGCTACAACTAAAAATAGCAGGTGCACCCGAGC CTCTGACAGTGACGGCACCATCCCTAACCATTGCGGAGAATATGGCTGACCTAATAGATGGGTACTGCCGGCTGGTGAATGGAACCTCGCAGTCATTTAT CATCAGACCTCAGAAAGAAGGTGAACGGGCTTTGCCATCAATACCAAAGTTGGCCAACAGCGAAAAGCAAGGCATGCGGACACACGCCGTCTCTGTGTCA GAAACAGATGATTATGCTGAGATTATAGATGAAGAAGATACTTACACCATGCCCTCAACCAGGGATTATGAGATTCAAAGAGAAAGAATAGAACTTGGAC GATGTATTGGAGAAGGCCAATTTGGAGATGTACATCAAGGCATTTATATGAGTCCAGAGAATCCAGCTTTGGCGGTTGCAATTAAAACATGTAAAAACTG TACTTCGGACAGCGTGAGAGAGAAATTTCTTCAAGAAGCCTTAACAATGCGTCAGTTTGACCATCCTCATATTGTGAAGCTGATTGGAGTCATCACAGAG AATCCTGTCTGGATAATCATGGAGCTGTGCACACTTGGAGAGCTGAGGTCATTTTTGCAAGTAAGGAAATACAGTTTGGATCTAGCATCTTTGATCCTGT ATGCCTATCAGCTTAGTACAGCTCTTGCATATCTAGAGAGCAAAAGATTTGTACACAGGGACATTGCTGCTCGGAATGTTCTGGTGTCCTCAAATGATTG TGTAAAATTAGGAGACTTTGGATTATCCCGATATATGGAAGATAGTACTTACTACAAAGCTTCCAAAGGAAAATTGCCTATTAAATGGATGGCTCCAGAG TCAATCAATTTTCGACGTTTTACCTCAGCTAGTGACGTATGGATGTTTGGTGTGTGTATGTGGGAGATACTGATGCATGGTGTGAAGCCTTTTCAAGGAG TGAAGAACAATGATGTAATCGGTCGAATTGAAAATGGGGAAAGATTACCAATGCCTCCAAATTGTCCTCCTACCCTCTACAGCCTTATGACGAAATGCTG GGCCTATGACCCCAGCAGGCGGCCCAGGTTTACTGAACTTAAAGCTCAGCTCAGCACAATCCTGGAGGAAGAGAAGGCTCAGCAAGAAGAGCGCATGAGG ATGGAGTCCAGAAGACAGGCCACAGTGTCCTGGGACTCCGGAGGGTCTGATGAAGCACCGCCCAAGCCCAGCAGACCGGGTTATCCCAGTCCGAGGTCCA GCGAAGGATTTTATCCCAGCCCACAGCACATGGTACAAACCAATCATTACCAGGTTTCTGGCTACCCTGGTTCACATGGAATCACAGCCATGGCTGGCAG CATCTATCCAGGTCAGGCATCTCTTTTGGACCAAACAGATTCATGGAATCATAGACCTCAGGAGATAGCAATGTGGCAGCCCAATGTGGAGGACTCTACA GTATTGGACCTGCGAGGGATTGGGCAAGTGTTGCCAACCCATCTGATGGAAGAGCGTCTAATCCGACAGCAACAGGAAATGGAAGAAGATCAGCGCTGGC TGGAAAAAGAGGAAAGATTTCTGAAACCTGATGTGAGACTCTCTCGAGGCAGTATTGACAGGGAGGATGGAAGTCTTCAGGGTCCGATTGGAAACCAACA TATATATCAGCCTGTGGGTAAACCAGATCCTGCAGCTCCACCAAAGAAACCGCCTCGCCCTGGAGCTCCCGGTCATCTGGGAAGCCTTGCCAGCCTCAGC AGCCCTGCTGACAGCTACAACGAGGGTGTCAAGCTTCAGCCCCAGGAAATCAGCCCCCCTCCTACTGCCAACCTGGACCGGTCGAATGATAAGGTGTACG AGAATGTGACGGGCCTGGTGAAAGCTGTCATCGAGATGTCCAGTAAAATCCAGCCAGCCCCACCAGAGGAGTATGTCCCTATGGTGAAGGAAGTCGGCTT GGCCCTGAGGACATTATTGGCCACTGTGGATGAGACCATTCCCCTCCTACCAGCCAGCACCCACCGAGAGATTGAGATGGCACAGAAGCTATTGAACTCT GACCTGGGTGAGCTCATCAACAAGATGAAACTGGCCCAGCAGTATGTCATGACCAGCCTCCAGCAAGAGTACAAAAAGCAAATGCTGACTGCTGCTCACG CCCTGGCTGTGGATGCCAAAAACTTACTCGATGTCATTGACCAAGCAAGACTGAAAATGCTTGGGCAGACGAGACCACACTGAGCCTCCCCTAGGAGCAC GTCTTGCTACCCTCTTTTGAAGATGTTCTCTAGCCTTCCACCAGCAGCGAGGAATTAACCCTGTGTCCTCAGTCGCCAGCACTTACAGCTCCAACTTTTT TGAATGACCATCTGGTTGAAAAATCTTTCTCATATAAGTTTAACCACACTTTGATTTGGGTTCATTTTTTGTTTTGTTTTTTTCAATCATGATATTCAGA AAAATCCAGGATCCAAAATGTGGCGTTTTTCTAAGAATGAAAATTATATGTAAGCTTTTAAGCATCATGAAGAACAATTTATGTTCACATTAAGATACGT TCTAAAGGGGGATGGCCAAGGGGTGACATCTTAATTCCTAAACTACCTTAGCTGCATAGTGGAAGAGGAGAGCATGAAGCAAAGAATTCCAGGAAACCCA AGAGGCTGAGAATTCTTTTGTCTACCATAGAATTATTATCCAGACTGGAATTTTTGTTTGTTAGAACACCCTTCAGTTGCAATATGCTAATCCCACTTTA CAAAGAATATAAAAGCTATATTTTGAAGACTTGAGTTATTTCAGAAAAAACTACAGCCCTTTTTGTCTTACCTGCCTTTTACTTTCGTGTGGATATGTGA AGCATTGGGTCGGGAACTAGCTGTAGAACACAACTAAAAACTCATGTCTTTTTTCACAGAATAATGTGCCAGTTTTTTGTAGCAATGTTATTTCTCTTGG AAGCAGAAATGCTTTGTACCAGAGCACCTCCAAACTGCATTGAGGAGAAGTTCCAGAACCATCCCCTTTTTCCATTTTTATATAATTTATAAAGAAAGAT TAAAGCCATGTTGACTATTTTACAGCCACTGGAGTTAACTAACCCTTCCTTGTATCTGTCTTCCCAGGAGAGAATGAAGCAAAACAGGAATTTGGTTTTC TTTTGATGTCCAGTTACACCATCCATTCTGTTAATTTTGAAAAAATATACCCTCCCTTTAGTTTGTTGGGGGATATAAATTATTCTCAGGAAGAATATAA TGAACTGTACAGTTACTTTGACCTATTAAAAAGGTGTTACCAGTAAAGTTCTTGTTGTAATATCCTTTCTTTTGGTTCTGTTTCTTCAGATGGCTTTTCA GGTGACTTGTCAGATAAGATAACATACAAGAGAGTTCCCAATAATTATCAGGAAGCCTCTCACTAATTAGTTTCTTTTTTTTTATTTAATCTTCTAAACA AGTCAAGGATGTTACCACAGAAGGGTAGCGTGGCGTAAGTTAACCTTTTCATTCCATCGTCACCTATTAAATGCAGAGCTCAGGATGGAAGTAGCAATGG TCTTCAATGAAAGAACCTTCCCCTGCGCAGGGTCTCACTATTCCGCTAACTCGGACTCAAAGCATAATTCACTTGTCAAGGAAATTGTTAGCATTTCCCA GGCCCACCCTAGTCTTGCTAGAATCTCTGTGAGCCACAGAGCAGGGAGCAAATAGAACTGAGATCTCCAGCACTAGGGACTCAGACTCCAAAGGGAGGGA TTTGAGTGAATTTTTTCCAGGTAAATACATGCTCTTATGTGC

PTK2.au.8.f (forward primer)
PTK2.u.r.53 (reverse primer)
Single exon skipping

Frame 0

SRTTVSPRREAWEEARLLSPSAAPSSSAFASRR RAAVRSLAVSPRLPGWSGVA*SRLTATSASQNMT DT*HLAK*WQLLTLTPT*ITHQIRVLRLTWVLV WNVLLVQWSEY*RSFIILKAIVSQPPGPVLSGM EMLLMSGASFRR*WTVTK*SMWPAMDSASVTCGQ RRFTGFTWIWASPV*GRSMSLLTHQRSGNMN*E FVICQKDF*TSLLKISQL*ISSINR*RAIIC*R *LIKWTRKLL*SWVV*KYGDHTGRCGAMH*KRSL TMKY*KKMLV*SDFFLRVYWILSRPKH*EN*SN KHLDNLPTLIEKKVF*NSLRSCLQSTDLIRNAS SVLLVQAGLFQWNWQSAQKKESVT*RTRAAIPHI LLTSLKCKPFSIQTVKTRTEKECYN*K*QVHPS L*Q*RHHP*PLRRIWLT**MGTAGW*MEPRSHL SSDLRKKVNGLCHQYQSWPTAKSKACGHTPSLCQ KQMIMLRL*MKKILTPCPQPGIMRFKEKE*NLD DVLEKANLEMYIKAFI*VQRIQLWRLQLKHVKT VLRTA*ERNFFKKP*QCVSLTILIL*S*LESSQR ILSG*SWSCAHLES*GHFCK*GNTVWI*HL*SC MPISLVQLLHI*RAKDLYTGTLLLGMFWCPQMI V*N*ETLDYPDIWKIVLTTKLPKENCLLNGWLQS QSIFDVLPQLVTYGCLVCVCGRY*CMV*SLFKE *RTMM*SVELKMGKDYQCLQIVLLPSTAL*RNA GPMTPAGGPGLLNLKLSSAQSWRKRRLSKKSA*G WSPEDRPQCPGTPEGLMKHRPSPADRVIPVRGP AKDFIPAHSTWYKPIITRFLATLVHMESQPWLA ASIQVRHLFWTKQIHGIIDLRR*QCGSPMWRTLQ YWTCEGLGKCCQPI*WKSV*SDSNRKWKKISAG WKKRKDF*NLM*DSLEAVLTGRMEVFRVRLETN IYISLWVNQILQLHQRNRLALELPVIWEALPASA ALLTATTRVSSFSPRKSAPLLLPTWTGRMIRCT RM*RAW*KLSSRCPVKSSQPHQRSMSLW*RKSA WP*GHYWPLWMRPFPSYQPAPTERLRWHRSY*TL TWVSSSTR*NWPSSMS*PASSKSTKSKC*LLLT PWLWMPKTYSMSLTKQD*KCLGRRDHTEPPLGA RLATLF*RCSLAFHQQRGINPVSSVASTYSSNFF E*PSG*KIFLI*V*PHFDLGSFFVLFFSIMIFR KIQDPKCGVFLRMKIICKLLSIMKNNLCSH*DT F*RGMAKG*HLNS*TTLAA*WKRRA*SKEFQETQ EAENSFVYHRIIIQTGIFVC*NTLQLQYANPTL QRI*KLYFEDLSYFRKNYSPFCLTCLLLSCGYV KHWVGN*L*NTTKNSCLFSQNNVPVFCSNVISLG SRNALYQSTSKLH*GEVPEPSPFSIFI*FIKKD *SHVDYFTATGVN*PFLVSVFPGENEAKQEFGF LLMSSYTIHSVNFEKIYPPFSLLGDINYSQEEYN ELYSYFDLLKRCYQ*SSCCNILSFGSVSSDGFS GDLSDKITYKRVPNNYQEASH*LVSFFLFNLLN KSRMLPQKGSVA*VNLFIPSSPIKCRAQDGSSNG LQ*KNLPLRRVSLFR*LGLKA*FTCQGNC*HFP GPP*SC*NLCEPQSREQIELRSPALGTQTPKGG I*VNFFQVNTCSYV

Frame 1

PGPL*ARGVRRGRKRGCCRPAPPRRRLPSLHGA EPRSEVLLCHPGCQAGVEWHDLG*LQPLPPRI* QIPSI*QNNGSCLP*PQLESHTKFEY*DSPGYWY GTFSWCNGASIKGLSLF*KQ**ANHLGQYYQAW RCY*CQGHHSEDSGQSQSKACGLLWIPPQSPAV RGGSLASRGYGRLQCEGEV*ACSPTRGVEI*IEN SLFAKRISKPVY*R*ANFEFLLSTGEERLYVRD S*SSGPGNCFEVGLSRNTAIILGDAGQCTRKEV *L*SIRKRCWFKAIFS*EFTGFCQGQNTKKTDPT NI*TICQP**RRKYSEIL*DPVSSLQI**GMLQ VCSWFKLDYFSGTGNRPRRRNQLPNGQGLQSHT SC*LHSSANHSVFKQ*RQGQKRNATTKNSRCTRA SDSDGTIPNHCGEYG*PNRWVLPAGEWNLAVIY HQTSERR*TGFAINTKVGQQRKARHADTRRLCV RNR*LC*DYR*RRYLHHALNQGL*DSKRKNRTWT MYWRRPIWRCTSRHLYESRESSFGGCN*NM*KL YFGQREREISSRSLNNASV*PSSYCEADWSHHR ESCLDNHGAVHTWRAEVIFASKEIQFGSSIFDPV CLSA*YSSCISREQKICTQGHCCSECSGVLK*L CKIRRLWIIPIYGR*YLLQSFQRKIAY*MDGSR VNQFSTFYLS**RMDVWCVYVGDTDAWCEAFSRS EEQ*CNRSN*KWGKITNASKLSSYPLQPYDEML GL*PQQAAQVY*T*SSAQHNPGGREGSARRAHE DGVQKTGHSVLGLRRV**STAQAQQTGLSQSEVQ RRILSQPTAHGTNQSLPGFWLPWFTWNHSHGWQ HLSRSGISFGPNRFMES*TSGDSNVAAQCGGLY SIGPARDWASVANPSDGRASNPTATGNGRRSALA GKRGKISET*CETLSRQY*QGGWKSSGSDWKPT YISACG*TRSCSSTKETASPWSSRSSGKPCQPQ QPC*QLQRGCQASAPGNQPPSYCQPGPVE**GVR ECDGPGESCHRDVQ*NPASPTRGVCPYGEGSRL GPEDIIGHCG*DHSPPTSQHPPRD*DGTEAIEL *PG*AHQQDETGPAVCHDQPPARVQKANADCCSR PGCGCQKLTRCH*PSKTENAWADETTLSLP*EH VLLPSFEDVL*PSTSSEELTLCPQSPALTAPTF LNDHLVEKSFSYKFNHTLIWVHFLFCFFQS*YSE KSRIQNVAFF*E*KLYVSF*AS*RTIYVHIKIR SKGGWPRGDILIPKLP*LHSGRGEHEAKNSRKP KRLRILLSTIELLSRLEFLFVRTPFSCNMLIPLY KEYKSYILKT*VISEKTTALFVLPAFYFRVDM* SIGSGTSCRTQLKTHVFFHRIMCQFFVAMLFLL EAEMLCTRAPPNCIEEKFQNHPLFPFLYNL*RKI KAMLTILQPLELTNPSLYLSSQERMKQNRNLVF F*CPVTPSILLILKKYTLPLVCWGI*IILRKNI MNCTVTLTY*KGVTSKVLVVISFLLVLFLQMAFQ VTCQIR*HTREFPIIIRKPLTN*FLFFYLIF*T SQGCYHRRVAWRKLTFSFHRHLLNAELRMEVAM VFNERTFPCAGSHYSANSDSKHNSLVKEIVSISQ AHPSLARISVSHRAGSK*N*DLQH*GLRLQREG FE*IFSR*IHALMC

Frame 2

PDHCEPAA*GVGGSAAAVAQRRPVVVCLRFTAP SRGPKSCCVTQAARLEWSGMISADCNLCLPEYD RYLASSKIMAAAYLDPNLNHTPNSSTKTHLGTG MERSPGAMERVLKVFHYFESNSEPTTWASIIRHG DATDVRGIIQKIVDSHKVKHVACYGFRLSHLRS EEVHWLHVDMGVSSVREKYELAHPPEEWKYELR IRYLPKGFLNQFTEDKPTLNFFYQQVKSDYMLEI ADQVDQEIALKLGCLEIRRSYWEMRGNALEKKS NYEVLEKDVGLKRFFPKSLLDSVKAKTLRKLIQ QTFRQFANLNREESILKFFEILSPVYRFDKECFK CALGSSWIISVELAIGPEEGISYLTDKGCNPTH LADFTQVQTIQYSNSEDKDRKGMLQLKIAGAPE PLTVTAPSLTIAENMADLIDGYCRLVNGTSQSFI IRPQKEGERALPSIPKLANSEKQGMRTHAVSVS ETDDYAEIIDEEDTYTMPSTRDYEIQRERIELG RCIGEGQFGDVHQGIYMSPENPALAVAIKTCKNC TSDSVREKFLQEALTMRQFDHPHIVKLIGVITE NPVWIIMELCTLGELRSFLQVRKYSLDLASLIL YAYQLSTALAYLESKRFVHRDIAARNVLVSSNDC VKLGDFGLSRYMEDSTYYKASKGKLPIKWMAPE SINFRRFTSASDVWMFGVCMWEILMHGVKPFQG VKNNDVIGRIENGERLPMPPNCPPTLYSLMTKCW AYDPSRRPRFTELKAQLSTILEEEKAQQEERMR MESRRQATVSWDSGGSDEAPPKPSRPGYPSPRS SEGFYPSPQHMVQTNHYQVSGYPGSHGITAMAGS IYPGQASLLDQTDSWNHRPQEIAMWQPNVEDST VLDLRGIGQVLPTHLMEERLIRQQQEMEEDQRW LEKEERFLKPDVRLSRGSIDREDGSLQGPIGNQH IYQPVGKPDPAAPPKKPPRPGAPGHLGSLASLS SPADSYNEGVKLQPQEISPPPTANLDRSNDKVY ENVTGLVKAVIEMSSKIQPAPPEEYVPMVKEVGL ALRTLLATVDETIPLLPASTHREIEMAQKLLNS DLGELINKMKLAQQYVMTSLQQEYKKQMLTAAH ALAVDAKNLLDVIDQARLKMLGQTRPH*ASPRST SCYPLLKMFSSLPPAARN*PCVLSRQHLQLQLF *MTIWLKNLSHISLTTL*FGFIFCFVFFNHDIQ KNPGSKMWRFSKNENYM*AFKHHEEQFMFTLRYV LKGDGQGVTS*FLNYLSCIVEEESMKQRIPGNP RG*EFFCLP*NYYPDWNFCLLEHPSVAIC*SHF TKNIKAIF*RLELFQKKLQPFLSYLPFTFVWICE ALGRELAVEHN*KLMSFFTE*CASFL*QCYFSW KQKCFVPEHLQTALRRSSRTIPFFHFYIIYKER LKPC*LFYSHWS*LTLPCICLPRRE*SKTGIWFS FDVQLHHPFC*F*KNIPSL*FVGGYKLFSGRI* *TVQLL*PIKKVLPVKFLL*YPFFWFCFFRWLF R*LVR*DNIQESSQ*LSGSLSLISFFFFI*SSKQ VKDVTTEG*RGVS*PFHSIVTY*MQSSGWK*QW SSMKEPSPAQGLTIPLTRTQSIIHLSRKLLAFP RPTLVLLESL*ATEQGANRTEISSTRDSDSKGRD LSEFFPGKYMLLC

Sequence PTK2.jAug05

TGAACTTAAAGCTCAGCTCAGCACAATCCTGGAGGAAGAGAAGGCTCAGCAAGAAGAGCGCATGAGGATGGAGTCCAGAAGACAGGCCACAGTGTCCTGG GACTCCGGAGGGTCTGATGAAGCACCGCCCAAGCCCAGCAGACCGGGTTATCCCAGTCCGAGGTCCAGCGAAGGATTTTATCCCAGCCCACAGCACATGG TACAAACCAATCATTACCAGGTTTCTGGCTACCCTGGTTCACATGGAATCACAGCCATGGCTGGCAGCATCTATCCAGGTCAGGCATCTCTTTTGGACCA AACAGATTCATGGAATCATAGACCTCAGGAGATAGCAATGTGGCAGCCCAATGTGGAGGACTCTACAGTATTGGACCTGCGAGGGATTGGGCAAGTGTTG CCAACCCATCTGATGGAAGAGCGTCTAATCCGACAGCAACAGGAAATGGAAGAAGATCAGCGCTGGCTGGAAAAAGAGGAAAGATTTCTGAAACCTGATG TGAGACTCTCTCGAGGCAGTATTGACAGGGAGGATGGAAGTCTTCAGGGTCCGATTGGAAACCAACATATATATCAGCCTGTGGGTAAACCAGATCCTGC AGCTCCACCAAAGAAACCGCCTCGCCCTGGAGCTCCCGGTCATCTGGGAAGCCTTGCCAGCCTCAGCAGCCCTGCTGACAGCTACAACGAGGGTGTCAAG CCATGGAGGCTTCAGCCCCAGGAAATCAGCCCCCCTCCTACTGCCAACCTGGACCGGTCGAATGATAAGGTGTACGAGAATGTGACGGGCCTGGTGAAAG CTGTCATCGAGATGTCCAGTAAAATCCAGCCAGCCCCACCAGAGGAGTATGTCCCTATGGTGAAGGAAGTCGGCTTGGCCCTGAGGACATTATTGGCCAC TGTGGATGAGACCATTCCCCTCCTACCAGCCAGCACCCACCGAGAGATTGAGATGGCACAGAAGCTATTGAACTCTGACCTGGGTGAGCTCATCAACAAG ATGAAACTGGCCCAGCAGTATGTCATGACCAGCCTCCAGCAAGAGTACAAAAACTTACTCGATGTCATTGACCAAGCAAGACTGAAAATGCTTGGGCAGA CGAGACCACACTGAGCCTCCCCTAGGAGCACGTCTTGCTACCCTCTTTTGAAGATGTTCTCTAGCCTTCCACCAGCAGCGAGGAATTAACCCTGTGTCCT CAGTCGCCAGCACTTACAGCTCCAACTTTTTTGAATGACCATCTGGTTGAAAAATCTTTCTCATATAAGTTTAACCACACTTTGATTTGGGTTCATTTTT TGTTTTGTTTTTTTCAATCATGATATTCAGAAAAATCCAGGATCCAAAATGTGGCGTTTTTCTAAGAATGAAAATTATATGTAAGCTTTTAAGCATCATG AAGAACAATTTATGTTCACATTAAGATACGTTCTAAAGGGGGATGGCCAAGGGGTGACATCTTAATTCCTAAACTACCTTAGCTGCATAGTGGAAGAGGA GAGCATGAAGCAAAGAATTCCAGGAAACCCAAGAGGCTGAGAATTCTTTTGTCTACCATAGAATTATTATCCAGACTGGAATTTTTGTTTGTTAGAACAC CCTTCAGTTGCAATATGCTAATCCCACTTTACAAAGAATATAAAAGCTATATTTTGAAGACTTGAGTTATTTCAGAAAAAACTACAGCCCTTTTTGTCTT ACCTGCCTTTTACTTTCGTGTGGATATGTGAAGCATTGGGTCGGGAACTAGCTGTAGAACACAACTAAAAACTCATGTCTTTTTTCACAGAATAATGTGC CAGTTTTTTGTAGCAATGTTATTTCTCTTGGAAGCAGAAATGCTTTGTACCAGAGCACCTCCAAACTGCATTGAGGAGAAGTTCCAGAACCATCCCCTTT TTCCATTTTTATATAATTTATAAAGAAAGATTAAAGCCATGTTGACTATTTTACAGCCACTGGAGTTAACTAACCCTTCCTTGTATCTGTCTTCCCAGGA GAGAATGAAGCAAAACAGGAATTTGGTTTTCTTTTGATGTCCAGTTACACCATCCATTCTGTTAATTTTGAAAAAATATACCCTCCCTTTAGTTTGTTGG GGGATATAAATTATTCTCAGGAAGAATATAATGAACTGTACAGTTACTTTGACCTATTAAAAAGGTGTTACCAGTAAAGTTCTTGTTGTAATATCCTTTC TTTTGGTTCTGTTTCTTCAGATGGCTTTTCAGGTGACTTGTCAGATAAGATAACATACAAGAGAGTTCCCAATAATTATCAGGAAGCCTCTCACTAATTA GTTTCTTTTTTTTTATTTAATCTTCTAAACAAGTCAAGGATGTTACCACAGAAGGGTAGCGTGGCGTAAGTTAACCTTTTCATTCCATCGTCACCTATTA AATGCAGAGCTCAGGATGGAAGTAGCAATGGTCTTCAATGAAAGAACCTTCCCCTGCGCAGGGTCTCACTATTCCGCTAACTCGGACTCAAAGCATAATT CACTTGTCAAGGAAATTGTTAGCATTTCCCAGGCCCACCCTAGTCTTGCTAGAATCTCTGTGAGCCACAGAGCAGGGAGCAAATAGAACTGAGATCTCCA GCACTAGGGACTCAGACTCCAAAGGGAGGGATTTGAGTGAATTTTTTCCAGGTAAATACATGCTCTTATGTGC

PTK2.au.8.f (forward primer)
PTK2.u.r.53 (reverse primer)
Single exon skipping

Frame 0

*T*SSAQHNPGGREGSARRAHEDGVQKTGHSVL GLRRV**STAQAQQTGLSQSEVQRRILSQPTAHG TNQSLPGFWLPWFTWNHSHGWQHLSRSGISFGP NRFMES*TSGDSNVAAQCGGLYSIGPARDWASV ANPSDGRASNPTATGNGRRSALAGKRGKISET*C ETLSRQY*QGGWKSSGSDWKPTYISACG*TRSC SSTKETASPWSSRSSGKPCQPQQPC*QLQRGCQ AMEASAPGNQPPSYCQPGPVE**GVRECDGPGES CHRDVQ*NPASPTRGVCPYGEGSRLGPEDIIGH CG*DHSPPTSQHPPRD*DGTEAIEL*PG*AHQQ DETGPAVCHDQPPARVQKLTRCH*PSKTENAWAD ETTLSLP*EHVLLPSFEDVL*PSTSSEELTLCP QSPALTAPTFLNDHLVEKSFSYKFNHTLIWVHF LFCFFQS*YSEKSRIQNVAFF*E*KLYVSF*AS* RTIYVHIKIRSKGGWPRGDILIPKLP*LHSGRG EHEAKNSRKPKRLRILLSTIELLSRLEFLFVRT PFSCNMLIPLYKEYKSYILKT*VISEKTTALFVL PAFYFRVDM*SIGSGTSCRTQLKTHVFFHRIMC QFFVAMLFLLEAEMLCTRAPPNCIEEKFQNHPL FPFLYNL*RKIKAMLTILQPLELTNPSLYLSSQE RMKQNRNLVFF*CPVTPSILLILKKYTLPLVCW GI*IILRKNIMNCTVTLTY*KGVTSKVLVVISF LLVLFLQMAFQVTCQIR*HTREFPIIIRKPLTN* FLFFYLIF*TSQGCYHRRVAWRKLTFSFHRHLL NAELRMEVAMVFNERTFPCAGSHYSANSDSKHN SLVKEIVSISQAHPSLARISVSHRAGSK*N*DLQ H*GLRLQREGFE*IFSR*IHALMC

Frame 1

ELKAQLSTILEEEKAQQEERMRMESRRQATVSW DSGGSDEAPPKPSRPGYPSPRSSEGFYPSPQHM VQTNHYQVSGYPGSHGITAMAGSIYPGQASLLDQ TDSWNHRPQEIAMWQPNVEDSTVLDLRGIGQVL PTHLMEERLIRQQQEMEEDQRWLEKEERFLKPD VRLSRGSIDREDGSLQGPIGNQHIYQPVGKPDPA APPKKPPRPGAPGHLGSLASLSSPADSYNEGVK PWRLQPQEISPPPTANLDRSNDKVYENVTGLVK AVIEMSSKIQPAPPEEYVPMVKEVGLALRTLLAT VDETIPLLPASTHREIEMAQKLLNSDLGELINK MKLAQQYVMTSLQQEYKNLLDVIDQARLKMLGQ TRPH*ASPRSTSCYPLLKMFSSLPPAARN*PCVL SRQHLQLQLF*MTIWLKNLSHISLTTL*FGFIF CFVFFNHDIQKNPGSKMWRFSKNENYM*AFKHH EEQFMFTLRYVLKGDGQGVTS*FLNYLSCIVEEE SMKQRIPGNPRG*EFFCLP*NYYPDWNFCLLEH PSVAIC*SHFTKNIKAIF*RLELFQKKLQPFLS YLPFTFVWICEALGRELAVEHN*KLMSFFTE*CA SFL*QCYFSWKQKCFVPEHLQTALRRSSRTIPF FHFYIIYKERLKPC*LFYSHWS*LTLPCICLPR RE*SKTGIWFSFDVQLHHPFC*F*KNIPSL*FVG GYKLFSGRI**TVQLL*PIKKVLPVKFLL*YPF FWFCFFRWLFR*LVR*DNIQESSQ*LSGSLSLI SFFFFI*SSKQVKDVTTEG*RGVS*PFHSIVTY* MQSSGWK*QWSSMKEPSPAQGLTIPLTRTQSII HLSRKLLAFPRPTLVLLESL*ATEQGANRTEIS STRDSDSKGRDLSEFFPGKYMLLC

Frame 2

NLKLSSAQSWRKRRLSKKSA*GWSPEDRPQCPG TPEGLMKHRPSPADRVIPVRGPAKDFIPAHSTW YKPIITRFLATLVHMESQPWLAASIQVRHLFWT KQIHGIIDLRR*QCGSPMWRTLQYWTCEGLGKCC QPI*WKSV*SDSNRKWKKISAGWKKRKDF*NLM *DSLEAVLTGRMEVFRVRLETNIYISLWVNQIL QLHQRNRLALELPVIWEALPASAALLTATTRVSS HGGFSPRKSAPLLLPTWTGRMIRCTRM*RAW*K LSSRCPVKSSQPHQRSMSLW*RKSAWP*GHYWP LWMRPFPSYQPAPTERLRWHRSY*TLTWVSSSTR *NWPSSMS*PASSKSTKTYSMSLTKQD*KCLGR RDHTEPPLGARLATLF*RCSLAFHQQRGINPVS SVASTYSSNFFE*PSG*KIFLI*V*PHFDLGSFF VLFFSIMIFRKIQDPKCGVFLRMKIICKLLSIM KNNLCSH*DTF*RGMAKG*HLNS*TTLAA*WKR RA*SKEFQETQEAENSFVYHRIIIQTGIFVC*NT LQLQYANPTLQRI*KLYFEDLSYFRKNYSPFCL TCLLLSCGYVKHWVGN*L*NTTKNSCLFSQNNV PVFCSNVISLGSRNALYQSTSKLH*GEVPEPSPF SIFI*FIKKD*SHVDYFTATGVN*PFLVSVFPG ENEAKQEFGFLLMSSYTIHSVNFEKIYPPFSLL GDINYSQEEYNELYSYFDLLKRCYQ*SSCCNILS FGSVSSDGFSGDLSDKITYKRVPNNYQEASH*L VSFFLFNLLNKSRMLPQKGSVA*VNLFIPSSPI KCRAQDGSSNGLQ*KNLPLRRVSLFR*LGLKA*F TCQGNC*HFPGPP*SC*NLCEPQSREQIELRSP ALGTQTPKGGI*VNFFQVNTCSYV