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Sequences

Sequence SHMT1.dAug05

CGGGGGCGGGGCCAAGCCCAAGCCTGGCGCGCAGAGTGCACCTTCCTGAGCTCGAGCGGTCCAGCGCCAAGTTCGGGGTTTGGGGTTGGAGCGGCTGGTC ACGTGGCTGGCCCGCGGCGGTGCGCGGGGCGTTGGGTCAGCGGGTCTGGGACTGGTGGCACCGGCGGCGGCGTAGGACGGAGGCGTCGCTAGGCAGCTTC GAACCAGTGCAATGACGATGCCAGTCAACGGGGCCCACAAGGATGCTGACCTGTGGTCCTCACATGACAAGATGCTGGCACAACCCCTCAAAGACAGTGA TGTTGAGGTTTACAACATCATTAAGAAGGAGAGTAACCGGCAGAGGGTTGGATTGGAGCTGATTGCCTCGGAGAATTTCGCCAGCCGAGCAGTTTTGGAG GCCCTAGGCTCTTGCTTAAATAACAAATACTCTGAGGGGTACCCGGGCCAGAGATACTATGGCGGGACTGAGTTTATTGATGAACTGGAGACCCTCTGTC AGAAGCGAGCCCTGCAGGCCTATAAGCTGGACCCACAGTGCTGGGGGGTCAACGTCCAGCCCTACTCAGGCTCCCCTGCAAACTTTGCTGTGTACACTGC CCTGGTGGAACCCCATGGGCGCATCATGGGCCTGGACCTTCCGGATGGGGGCCACCTGACCCATGGGTTCATGACAGACAAGAAGAAAATCTCTGCCACG TCCATCTTCTTTGAATCTATGCCCTACAAGGTGAACCCAGATACTGGCTACATCAACTATGACCAGCTGGAGGAGAACGCACGCCTCTTCCACCCGAAGC TGATCATCGCAGGAACCAGCTGCTACTCCCGAAACCTGGAATATGCCCGGCTACGGAAGATTGCAGATGAGAACGGGGCGTATCTCATGGCGGACATGGC TCACATCAGCGGGCTGGTGGCGGCTGGCGTGGTGCCCTCCCCATTTGAACACTGCCATGTGGTGACCACCACCACTCACAAGACCCTGCGAGGCTGCCGA GCTGGCATGATCTTCTACAGGAAAGGGGTTGCTGTGGCACTGAAGCAAGCTATGACTCTGGAATTTAAAGTTTATCAACACCAGGTGGTGGCCAACTGCA GGGCTCTGTCTGAGGCCCTGACGGAGCTGGGCTACAAAATAGTCACAGGTGGTTCTGACAACCATTTGATCCTTGTGGATCTCCGTTCCAAAGGCACAGA TGGTGGAAGGGCTGAGAAGGTGCTAGAAGCCTGTTCTATTGCCTGCAACAAGAACACCTGTCCAGGTGACAGAAGCGCTCTGCGGCCCAGTGGACTGCGG CTGGGGACCCCAGCACTGACGTCCCGTGGACTTTTGGAAAAAGACTTCCAAAAAGTAGCCCACTTTATTCACAGAGGGATAGAGCTGACCCTGCAGATCC AGAGCGACACTGGTGTCAGAGCCACCCTGAAAGAGTTCAAGGAGAGACTGGCAGGGGATAAGTACCAGGCGGCCGTGCAGGCTCTCCGGGAGGAGGTTGA GAGCTTCGCCTCTCTCTTCCCTCTGCCTGGCCTGCCTGACTTCTAAAGGAGCGGGCCCACTCTGGACCCACCTGGCGCCACAGAGGAAGCTGCCTGCCGG AGGACCCCCACCTGAGAGATGGATGAGCTGCTCCAAAGGGGAACTGTTGACACTCGGGCCCTTTGAGGGGGTTTCTTTTGGACTTTTTTCATGTTTTCTT CACAAATCAAAATTTGTTTAAGTCTCATTGTTAGTAATTCTGGGACAGGTTATTAAAGGATTTAAATTTGAACCTGGCTTTCTCACAGCTGGACATAATT CTAGGAAAATAAAATACTATGTCGCCACTTGGTCATAATCATTTAGATGGTGGTGTAGGGCAAAGCTGTTAGAAAGATTGTAGCGTTTTACTCTCCCTGG GCTTTCCTCCGCCTTGCTGCAACAGAGAGGAAATGCCCATGTCCACAGCTTGTACACACTGCCCCCTCACTATCTTGTTATCCAGTGGCATGCCAAAGGA GAACTGAATTAGCTTCTGAGGCTTCTGCTGTAAATCAGAAGTGTATGTTAGTCAAGAGTAAACAAGATGCACCCAGTATGGTGGGAGGGTTTTGCTGTCA GTAGCTCAAAGTATGGTGTAGAAATGGCCTCCTCCCTCCATCCTGGGAAGTCCCAGTCCCATCCTGGTGTGAGAATCAACCAGGCTTTCCTGCTCCACCT GAGATAACCAACTCCCTCCCGTAATCAGGAAGCCAAATGTCACCTTCCCAAAGAAATTTTATTTTCACGTAGCTGAAGTGCAAAACATAGATGACCATTT TTAATAAGCACAATCAAATTTTTAACCACAGAATGTCTACAAGAATTATAGCTTTAAAAAATACAACCAATTTTTATATTTCAAAAATATTTGAACTCAA ATAAATTAATTTCTTAAAAAGTACACTTCTCATACTATGTGTTTGGCATTGACTCTGGTTAACTCTTGTGCAGCAACACCTACGAGATGCTATCACACAC GCGCCAGCTGCACTCCTGCACCAGCCGCTGCAACGACTGCACCTCACGTGGTGGCGGGGACTCACATTCACAAGACGGGACGGGAACATGCAGTAATAAA ATAGCTTTTTAAAAAATAACACATATAAATTCATCACATAGGATTATGCTTTTCACTGCAACCTTGAAGGTGGCATTTATCTCAATCTGTGACTCCAGCT ATTTAGTGGCAGACAATCCCGGTGAGGCAGCCTGTGGTCCCACCTTGTAGGTGGCAGCCCCTGCAATCTGGGGTCTAGCCTGTCGGCGTTTGCTTTATTG TGGGTGGAGCAGCTGCTGCAGAATCTGTGAGTTGACTCACCAGTCCCTACAGTTCTGCTACCAAAGTGGTCTTCAATTTAGGGGCCTGACCCCTCCCCAG CTGGAGACAAGCTGCCATCTCCAGGGCTCGCTGTAAACAGCCTCCACTCTTGGGCTGAATAAGCACTCCCAAGAAGCTGGGCCCCTACCAACAGATTAAT CGGCTGCAGAGTGCTCATCCCCTCCCCACACCCCCATCCACCCCTGCCTCCTCCTCCTTTCCACTGCTTTAACCAAACCTTAATGAGGTAAGGCCAGGCT AGGTCAGGGCTCAAAGGAAAATGGGCTCAGGAAAACTTAGTTTGCCCAGCCTTGTCAGCTGAGATTGGTTTTGCTTTCCAGCCAGAAGTAGAAACTTGAT GCTTTTTCCTTTTTGCAGTGTTAAAAACCATATCTCCAGTTTTATTATGTTCCATCACAGTTTTGGGGCAAAGCCAGAAAGCTGACTTGTTTGCTTCAAA ACACAAACCAAGGCACTGTCTTCTGTGCTTTTCAGGGAGGTGGGAAGGTAAAGGAGACTGCCCACTATAGGGGCTCAGTTCCCCCAAAGGCCTTGCACAC CTTCTGATAAACCCCCTGGCTGAGGCAATCCTACTGGCTGTGCAGACAAAGATGTGACAGCAGCACTAGATGCTGACCCAACAAGCAGTGCTGGGCCTGG GCGGTGGGGCATGTTCAGCACTGCGGGCAGCAGTCAGCACATGCAAGACATTCAGTGAGCAGAGGGCTGCCCTCGGCCTCCAACTGCAGCCGCCCTGGCG CGTGAAATGAAATTGGTGGGCCTTCCTAGGATTGTAAAATGGGACTTGTCATTTGTGTGAGGTTGAAATCTACTATACTTTGAATATGGCAACTGTTTTC AACCACTGAAGCAAAGAAGGTAAAGGCCAGATGGGGAATGAAAGGTAAGGTACTGTGGTCTATTTCCAAATTTGGGAGCAATGTGTGTAACTTCAACTGT TGGAGTAAATGAGTTATGGAAAAGCCACAATTTCTAAAATCCCAAGGTTCTACCAAAGGAGTGAGGTTCCTGGGGAAACAGGCATGAGGCAGCAGCTCAG GCTGATGTGTCCTGTGCGATATAAAAAGCTGTGGCCTGATCTCTAAAGTGGGGCTTTTGAAAATCACCAGTCCACAACTACCTGCCTGGCTAGTTTAACC TAGACTGCTTCAGAAGCAAGGGAGGGGGATAATGGACTGGAGAAAAAGCTGACACAAAACTCAAGACAACCAGTTTCAGTACCTGGGCCCTCAAAGCAAT AGTGAGGACTGAGAAAGTAGCAAGGGGCACTGATGTCATCTATTTTCTGGGAAGTGTAATTTTTAATGGTTTCATTAAAAACTTACTAACCTTCTACTGG AAGAATACCTCAAATGAAAAATTTTTTTTTTTTGAGACAGTTTTCGCTCTTGTTGCCCAGGGTGGAGTGCAGTGGCGCGATCTCGGCTCACTGTAACCTC TGCCTCCTGGGTTCAAGTGATTCTCCTGCCTCAGCCTCCCGAGTAAGTGGGATTACAGGCACGCACCACCAGACCTGGCTAACTTTCTTTGTATTTTTAG TAGAGATGGGGTTTCACCATGTTGGCCAGGCTGGTCTCCAACTCCTGACCTCAGGTGATCCGCCCACCTCGGCCTCCCAAAGTGTTGGGATTACAGGCGT GAGCCACTGCGCCCGGCCTCAACTGAAAATTCTTGATGAGATTAGTCCCACTGCATAGGTCTCAGCACTGAGGTTTAGAGATGTGAGACTCAGAGTAGGT TTCAGTTCCCCGGAGTGAAGGGGGCATAGGGAGGGGCAGGGCTTCCCAGTGTTAACTGCCTCTGCCCAAATCATGACTCAGTCCTGGTACCACGTCAAGA TCCCTTTGAAAGGAAACATACTTTGCATCAAGTAATCCTTTGAAACTGCCACATTGAAAAGTTAGTTTTTAACCCAAATATAAATCTGGGAGCTAAACAC TAGTCACGAAGCCATCTTTACTTAATGAAGAACCACCTTGAGGGAGACCAGCCCTGCTTGCCACTTACATGGCTGCCCCTGAGACGGTCTGTTCTGTTCA TGCCCCAGCAGCCTTTTGCCTTAGGTTTCAGGACCTTCTGAACCACCCTTGAGGGTGTGTGCCAGCAAATACCTCAAGTGGACTTCTACTTAGGACCACT TGAGGGCACAACTTCAGCTTCTAGAAGGCCAAGTGTGGCTGCAATTCAGGATCTGCCCCAAACTCATCAGCCCTTCCCAAGTTTCCCAGGTGAGAACAGT ATGAGCTGTGAATTTCTGTGTTGGTGTGACAGGAATGCCACAGTGGTGGTGCCAGTGATCACTTTCTGACCTTCCTTCCTTCTCAGATAAAATGTTGCAC AGTTATGGCCTTGGAGGCCTAAGTAGACAGCAATTAACCAGCCAGTGAGAAAACGAATCAGCATCTAGAACAGGAGGGGAGGGAACCGTAACAGATAAGG GAGCCCACGATTAGAAGCAGGCTCCCTGCACAGCCTCTGCCTCCAGCGCAAGTAACAGACGTTTCACATTTAAAGGCTGCCAAGGTATTGCTGGCGACTC TCCTGTCTGTGAACATGCGCAACGGGCACAGGTACAGAGAACCAATGAGATGTAGGCAGCACCATCTGCCACAAAAGGTTCTCCATCGCAGCCCCAGGAG CAAAATCAAACACCTCTGATGGGAGCAAGGACCCTGAACAGAAGCCAACGGCTGGAGCCAACGGGCCATGCCCTTAAAACGAGGACTTGGACAAGGAGGG AACCACTGGAGTGACGTGATGCAAGCCCTCTTCGGTCACTCTTGGCTTCCACCAGGGGTCTCTGCCTCCTTGATGAGAAGAGAAGCTGGCTGGCTCCATG ATGACCTCGATGGCGTACTACAGGCCGATGGGCAGGCCGGGCCTCAGCAGCAGGCGCCAACAGAAGCACACCCAGGGCTGATTCAGGTCCATTTCAGCAT AATTTTTGTGGAACAAATTAAAGAAGCAGTAATACCAGAGCTGTCCCCACTTAGGGAATAAAATTTTCAAGGCTGCAAGAAACTCTCTGTCTACAAACCA GTGGCCATTGCCTAAGGAGGCTCGGGGAGGCCAGGGGATCACTATCAGTGGGATAGGTGCTGGCGGAACCAGGGGGTGTCTCCTTCCCAGTTTCAAACTT GTCCTCAGAAGGGGGACTCAACTGGGGACGGCCGTGAGCTGTGGTGACTGAGGTGCACACAGCCTCCCAGAGGCAGAACCTGAAGAGCGCCACGTGGTAG CTCATCCAGGAAATCCGTGCTATTTGAGTTCTCATCGGCTGGGCCAACCACCTGCACCAGCTGACTAAACAGAACCGTTGCTGTCCCAAGCCCACGAGAC CCAGTGAGAGG

SHMT1.u.f.24 (forward primer)
SHMT1.u.r.18 (reverse primer)
Single exon skipping

Frame 0

RGRGQAQAWRAECTFLSSSGPAPSSGFGVGAAG HVAGPRRCAGRWVSGSGTGGTGGGVGRRRR*AAS NQCNDDASQRGPQGC*PVVLT*QDAGTTPQRQ* C*GLQHH*EGE*PAEGWIGADCLGEFRQPSSFG GPRLLLK*QIL*GVPGPEILWRD*VY**TGDPLS EASPAGL*AGPTVLGGQRPALLRLPCKLCCVHC PGGTPWAHHGPGPSGWGPPDPWVHDRQEENLCH VHLL*IYALQGEPRYWLHQL*PAGGERTPLPPEA DHRRNQLLLPKPGICPATEDCR*ERGVSHGGHG SHQRAGGGWRGALPI*TLPCGDHHHSQDPARLP SWHDLLQERGCCGTEASYDSGI*SLSTPGGGQLQ GSV*GPDGAGLQNSHRWF*QPFDPCGSPFQRHR WWKG*EGARSLFYCLQQEHLSR*QKRSAAQWTA AGDPSTDVPWTFGKRLPKSSPLYSQRDRADPADP ERHWCQSHPERVQGETGRG*VPGGRAGSPGGG* ELRLSLPSAWPA*LLKERAHSGPTWRHRGSCLP EDPHLRDG*AAPKGNC*HSGPLRGFLLDFFHVFF TNQNLFKSHC**FWDRLLKDLNLNLAFSQLDII LGK*NTMSPLGHNHLDGGVGQSC*KDCSVLLSL GFPPPCCNREEMPMSTACTHCPLTILLSSGMPKE N*ISF*GFCCKSEVYVSQE*TRCTQYGGRVLLS VAQSMV*KWPPPSILGSPSPILV*ESTRLSCST *DNQLPPVIRKPNVTFPKKFYFHVAEVQNIDDHF **AQSNF*PQNVYKNYSFKKYNQFLYFKNI*TQ IN*FLKKYTSHTMCLALTLVNSCAATPTRCYHT RASCTPAPAAATTAPHVVAGTHIHKTGREHAVIK *LFKK*HI*IHHIGLCFSLQP*RWHLSQSVTPA I*WQTIPVRQPVVPPCRWQPLQSGV*PVGVCFI VGGAAAAESVS*LTSPYSSATKVVFNLGA*PLPS WRQAAISRARCKQPPLLG*ISTPKKLGPYQQIN RLQSAHPLPTPPSTPASSSFPLL*PNLNEVRPG *VRAQRKMGSGKLSLPSLVS*DWFCFPARSRNLM LFPFCSVKNHISSFIMFHHSFGAKPES*LVCFK TQTKALSSVLFREVGR*RRLPTIGAQFPQRPCT PSDKPPG*GNPTGCADKDVTAALDADPTSSAGPG RWGMFSTAGSSQHMQDIQ*AEGCPRPPTAAALA REMKLVGLPRIVKWDLSFV*G*NLLYFEYGNCF QPLKQRR*RPDGE*KVRYCGLFPNLGAMCVTSTV GVNELWKSHNF*NPKVLPKE*GSWGNRHEAAAQ ADVSCAI*KAVA*SLKWGF*KSPVHNYLPG*FN LDCFRSKGGG*WTGEKADTKLKTTSFSTWALKAI VRTEKVARGTDVIYFLGSVIFNGFIKNLLTFYW KNTSNEKFFFFETVFALVAQGGVQWRDLGSL*P LPPGFK*FSCLSLPSKWDYRHAPPDLANFLCIFS RDGVSPCWPGWSPTPDLR*SAHLGLPKCWDYRR EPLRPASTENS**D*SHCIGLSTEV*RCETQSR FQFPGVKGA*GGAGLPSVNCLCPNHDSVLVPRQD PFERKHTLHQVIL*NCHIEKLVFNPNINLGAKH *SRSHLYLMKNHLEGDQPCLPLTWLPLRRSVLF MPQQPFALGFRTF*TTLEGVCQQIPQVDFYLGPL EGTTSASRRPSVAAIQDLPQTHQPFPSFPGENS MSCEFLCWCDRNATVVVPVITF*PSFLLR*NVA QLWPWRPK*TAINQPVRKRISI*NRRGGNRNR*G SPRLEAGSLHSLCLQRK*QTFHI*RLPRYCWRL SCL*TCATGTGTENQ*DVGSTICHKRFSIAAPG AKSNTSDGSKDPEQKPTAGANGPCP*NEDLDKEG TTGVT*CKPSSVTLGFHQGSLPP**EEKLAGSM MTSMAYYRPMGRPGLSSRRQQKHTQG*FRSISA *FLWNKLKKQ*YQSCPHLGNKIFKAARNSLSTNQ WPLPKEARGGQGITISGIGAGGTRGCLLPSFKL VLRRGTQLGTAVSCGD*GAHSLPEAEPEERHVV AHPGNPCYLSSHRLGQPPAPAD*TEPLLSQAHET Q*E

Frame 1

GGGAKPKPGAQSAPS*ARAVQRQVRGLGLERLV TWLARGGARGVGSAGLGLVAPAAA*DGGVARQL RTSAMTMPVNGAHKDADLWSSHDKMLAQPLKDSD VEVYNIIKKESNRQRVGLELIASENFASRAVLE ALGSCLNNKYSEGYPGQRYYGGTEFIDELETLC QKRALQAYKLDPQCWGVNVQPYSGSPANFAVYTA LVEPHGRIMGLDLPDGGHLTHGFMTDKKKISAT SIFFESMPYKVNPDTGYINYDQLEENARLFHPK LIIAGTSCYSRNLEYARLRKIADENGAYLMADMA HISGLVAAGVVPSPFEHCHVVTTTTHKTLRGCR AGMIFYRKGVAVALKQAMTLEFKVYQHQVVANC RALSEALTELGYKIVTGGSDNHLILVDLRSKGTD GGRAEKVLEACSIACNKNTCPGDRSALRPSGLR LGTPALTSRGLLEKDFQKVAHFIHRGIELTLQI QSDTGVRATLKEFKERLAGDKYQAAVQALREEVE SFASLFPLPGLPDF*RSGPTLDPPGATEEAACR RTPT*EMDELLQRGTVDTRAL*GGFFWTFFMFS SQIKICLSLIVSNSGTGY*RI*I*TWLSHSWT*F *ENKILCRHLVIII*MVV*GKAVRKIVAFYSPW AFLRLAATERKCPCPQLVHTAPSLSCYPVACQR RTELASEASAVNQKCMLVKSKQDAPSMVGGFCCQ *LKVWCRNGLLPPSWEVPVPSWCENQPGFPAPP EITNSLP*SGSQMSPSQRNFIFT*LKCKT*MTI FNKHNQIFNHRMSTRIIALKNTTNFYISKIFELK *INFLKSTLLILCVWH*LWLTLVQQHLRDAITH APAALLHQPLQRLHLTWWRGLTFTRRDGNMQ** NSFLKNNTYKFIT*DYAFHCNLEGGIYLNL*LQL FSGRQSR*GSLWSHLVGGSPCNLGSSLSAFALL WVEQLLQNL*VDSPVPTVLLPKWSSI*GPDPSP AGDKLPSPGLAVNSLHSWAE*ALPRSWAPTNRLI GCRVLIPSPHPHPPLPPPPFHCFNQTLMR*GQA RSGLKGKWAQENLVCPALSAEIGFAFQPEVET* CFFLFAVLKTISPVLLCSITVLGQSQKADLFASK HKPRHCLLCFSGRWEGKGDCPL*GLSSPKGLAH LLINPLAEAILLAVQTKM*QQH*MLTQQAVLGL GGGACSALRAAVSTCKTFSEQRAALGLQLQPPWR VK*NWWAFLGL*NGTCHLCEVEIYYTLNMATVF NH*SKEGKGQMGNER*GTVVYFQIWEQCV*LQL LE*MSYGKATISKIPRFYQRSEVPGETGMRQQLR LMCPVRYKKLWPDL*SGAFENHQSTTTCLASLT *TASEAREGDNGLEKKLTQNSRQPVSVPGPSKQ **GLRK*QGALMSSIFWEV*FLMVSLKTY*PSTG RIPQMKNFFFLRQFSLLLPRVECSGAISAHCNL CLLGSSDSPASASRVSGITGTHHQTWLTFFVFL VEMGFHHVGQAGLQLLTSGDPPTSASQSVGITGV SHCARPQLKILDEISPTA*VSALRFRDVRLRVG FSSPE*RGHREGQGFPVLTASAQIMTQSWYHVK IPLKGNILCIK*SFETATLKS*FLTQI*IWELNT SHEAIFT**RTTLRETSPACHLHGCP*DGLFCS CPSSLLP*VSGPSEPPLRVCASKYLKWTST*DH LRAQLQLLEGQVWLQFRICPKLISPSQVSQVRTV *AVNFCVGVTGMPQWWCQ*SLSDLPSFSDKMLH SYGLGGLSRQQLTSQ*ENESASRTGGEGTVTDK GAHD*KQAPCTASASSASNRRFTFKGCQGIAGDS PVCEHAQRAQVQRTNEM*AAPSATKGSPSQPQE QNQTPLMGARTLNRSQRLEPTGHALKTRTWTRR EPLE*RDASPLRSLLASTRGLCLLDEKRSWLAP* *PRWRTTGRWAGRASAAGANRSTPRADSGPFQH NFCGTN*RSSNTRAVPT*GIKFSRLQETLCLQT SGHCLRRLGEARGSLSVG*VLAEPGGVSFPVSNL SSEGGLNWGRP*AVVTEVHTASQRQNLKSATW* LIQEIRAI*VLIGWANHLHQLTKQNRCCPKPTR PSER

Frame 2

GAGPSPSLARRVHLPELERSSAKFGVWGWSGWS RGWPAAVRGALGQRVWDWWHRRRRRTEASLGSF EPVQ*RCQSTGPTRMLTCGPHMTRCWHNPSKTV MLRFTTSLRRRVTGRGLDWS*LPRRISPAEQFWR P*ALA*ITNTLRGTRARDTMAGLSLLMNWRPSV RSEPCRPISWTHSAGGSTSSPTQAPLQTLLCTL PWWNPMGASWAWTFRMGAT*PMGS*QTRRKSLPR PSSLNLCPTR*TQILATSTMTSWRRTHASSTRS *SSQEPAATPETWNMPGYGRLQMRTGRISWRTW LTSAGWWRLAWCPPHLNTAMW*PPPLTRPCEAAE LA*SSTGKGLLWH*SKL*LWNLKFINTRWWPTA GLCLRP*RSWATK*SQVVLTTI*SLWISVPKAQ MVEGLRRC*KPVLLPATRTPVQVTEALCGPVDCG WGPQH*RPVDFWKKTSKK*PTLFTEG*S*PCRS RATLVSEPP*KSSRRDWQGISTRRPCRLSGRRL RASPLSSLCLACLTSKGAGPLWTHLAPQRKLPAG GPPPERWMSCSKGELLTLGPFEGVSFGLFSCFL HKSKFV*VSLLVILGQVIKGFKFEPGFLTAGHN SRKIKYYVATWS*SFRWWCRAKLLERL*RFTLPG LSSALLQQRGNAHVHSLYTLPPHYLVIQWHAKG ELN*LLRLLL*IRSVC*SRVNKMHPVWWEGFAV SSSKYGVEMASSLHPGKSQSHPGVRINQAFLLHL R*PTPSRNQEAKCHLPKEILFSRS*SAKHR*PF LISTIKFLTTECLQEL*L*KIQPIFIFQKYLNS NKLIS*KVHFSYYVFGIDSG*LLCSNTYEMLSHT RQLHSCTSRCNDCTSRGGGDSHSQDGTGTCSNK IAF*KITHINSSHRIMLFTATLKVAFISICDSS YLVADNPGEAACGPTL*VAAPAIWGLACRRLLYC GWSSCCRICELTHQSLQFCYQSGLQFRGLTPPQ LETSCHLQGSL*TASTLGLNKHSQEAGPLPTD* SAAECSSPPHTPIHPCLLLLSTALTKP**GKARL GQGSKENGLRKT*FAQPCQLRLVLLSSQK*KLD AFSFLQC*KPYLQFYYVPSQFWGKARKLTCLLQ NTNQGTVFCAFQGGGKVKETAHYRGSVPPKALHT F**TPWLRQSYWLCRQRCDSSTRC*PNKQCWAW AVGHVQHCGQQSAHARHSVSRGLPSASNCSRPG A*NEIGGPS*DCKMGLVICVRLKSTIL*IWQLFS TTEAKKVKARWGMKGKVLWSISKFGSNVCNFNC WSK*VMEKPQFLKSQGSTKGVRFLGKQA*GSSS G*CVLCDIKSCGLISKVGLLKITSPQLPAWLV*P RLLQKQGRGIMDWRKS*HKTQDNQFQYLGPQSN SED*ESSKGH*CHLFSGKCNF*WFH*KLTNLLL EEYLK*KIFFF*DSFRSCCPGWSAVARSRLTVTS ASWVQVILLPQPPE*VGLQARTTRPG*LSLYF* *RWGFTMLARLVSNS*PQVIRPPRPPKVLGLQA *ATAPGLN*KFLMRLVPLHRSQH*GLEM*DSE*V SVPRSEGGIGRGRASQC*LPLPKS*LSPGTTSR SL*KETYFASSNPLKLPH*KVSF*PKYKSGS*T LVTKPSLLNEEPP*GRPALLATYMAAPETVCSVH APAAFCLRFQDLLNHP*GCVPANTSSGLLLRTT *GHNFSF*KAKCGCNSGSAPNSSALPKFPR*EQ YEL*ISVLV*QECHSGGASDHFLTFLPSQIKCCT VMALEA*VDSN*PASEKTNQHLEQEGREP*QIR EPTIRSRLPAQPLPPAQVTDVSHLKAAKVLLAT LLSVNMRNGHRYREPMRCRQHHLPQKVLHRSPRS KIKHL*WEQGP*TEANGWSQRAMPLKRGLGQGG NHWSDVMQALFGHSWLPPGVSASLMRREAGWLH DDLDGVLQADGQAGPQQQAPTEAHPGLIQVHFSI IFVEQIKEAVIPELSPLRE*NFQGCKKLSVYKP VAIA*GGSGRPGDHYQWDRCWRNQGVSPSQFQT CPQKGDSTGDGRELW*LRCTQPPRGRT*RAPRGS SSRKSVLFEFSSAGPTTCTS*LNRTVAVPSPRD PVR

Sequence SHMT1.eAug05

TTTTTTTTCAGATGGAGTCTCGCTCTGTTGCCCAGGCTGGAGTGCTGGAGTGCAGTGGCACAATCTCGGCTCACTGCAAGCTCCACCTCCCGGGTTCACG CCATTCTCCTGCCTCAGCCTCCCAAGTAGCTGGGATTACAGGCGCCCGCCACCATATCTGGCTAATTTTTTGCATTTTTAGTAGAGACGGGGTTTCACCG TGTTAGCCAGGATGGTCTCGATCTCCTGACCTCATGATCTGCCCGTCTCAGCCTCCCAAAGTGCTGGGATTACAGGTGTGAGCCACCGCGCCCAGCCCCT ACTTCTTGTTTTTCAATGTTGTATCCAGTGACCTTGCTAAATTTGCTTATTCTAACAGTTGGCCCCTAGCATCTTGATTTTCTAGGTATATTGTGTTGTC TGTGTGTAATGTCAGTTTTTAAATTTCTTCTTTCCTAATCGTTATGCCTTTTGTTTCTTTTTCTTGCCTCATTATACTTGGAATAGAAGTAGTATTAGCA GGCATTTTGGTCTTGTTCCTGAAGGTAACCATTTTGGTTTCCATTGTGTGGTCCCGTCACATTTGTTTCATTCTGCAGGTGCTTTTTGGTTCCTTCTCAG CAGGTAGTGCCCCAGCAGCAGGGCTCACTACAACCCTGGGACTCCTCAGGGACTCCTGGGTCTGTGAGCCATACTTGGTTGAGCAGGCTGAACACACCAG TATCCATCCTGTCTCACCCTCAAGTCCTCATCTAGGACATCATTCCTCTTGTCATGGTGGGTGGAGCCAGTTCAAGTGGGAGGACAGGAGGCAGGTGTGG TTGGAGGAAGCAGCCTGAACCTGCCTCCCTGACATTCCACAGGTGAACCCAGATACTGGCTACATCAACTATGACCAGCTGGAGGAGAACGCACGCCTCT TCCACCCGAAGCTGATCATCGCAGGAACCAGCTGCTACTCCCGAAACCTGGAATATGCCCGGCTACGGAAGATTGCAGATGAGAACGGGGCGTATCTCAT GGCGGACATGGCTCACATCAGCGGGCTGGTGGCGGCTGGCGTGGTGCCCTCCCCATTTGAACACTGCCATGTGGTGACCACCACCACTCACAAGACCCTG CGAGGCTGCCGAGCTGGCATGATCTTCTACAGGAAAGGAGTGAAAAGTGTGGATCCCAAGACTGGCAAAGAGATTCTGTACAACCTGGAGTCTCTTATCA ATTCTGCTGTGTTCCCTGGCCTGCAGGGAGGTCCCCACAACCACGCCATTGCTGGGGTTGCTGTGGCACTGAAGCAAGCTATGACTCTGGAATTTAAAGT TTATCAACACCAGGTGGTGGCCAACTGCAGGGCTCTGTCTGAGGCCCTGACGGAGCTGGGCTACAAAATAGTCACAGGTGGTTCTGACAACCATTTGATC CTTGTGGATCTCCGTTCCAAAGGCACAGATGGTGGAAGGGCTGAGAAGGTGCTAGAAGCCTGTTCTATTGCCTGCAACAAGAACACCTGTCCAGGTGACA GAAGCGCTCTGCGGCCCAGTGGACTGCGGCTGGGGACCCCAGCACTGACGTCCCGTGGACTTTTGGAAAAAGACTTCCAAAAAGTAGCCCACTTTATTCA CAGAGGGATAGAGCTGACCCTGCAGATCCAGAGCGACACTGGTGTCAGAGCCACCCTGAAAGAGTTCAAGGAGAGACTGGCAGGGGATAAGTACCAGGCG GCCGTGCAGGCTCTCCGGGAGGAGGTTGAGAGCTTCGCCTCTCTCTTCCCTCTGCCTGGCCTGCCTGACTTCTAAAGGAGCGGGCCCACTCTGGACCCAC CTGGCGCCACAGAGGAAGCTGCCTGCCGGAGGACCCCCACCTGAGAGATGGATGAGCTGCTCCAAAGGGGAACTGTTGACACTCGGGCCCTTTGAGGGGG TTTCTTTTGGACTTTTTTCATGTTTTCTTCACAAATCAAAATTTGTTTAAGTCTCATTGTTAGTAATTCTGGGACAGGTTATTAAAGGATTTAAATTTGA ACCTGGCTTTCTCACAGCTGGACATAATTCTAGGAAAATAAAATACTATGTCGCCACTTGGTCATAATCATTTAGATGGTGGTGTAGGGCAAAGCTGTTA GAAAGATTGTAGCGTTTTACTCTCCCTGGGCTTTCCTCCGCCTTGCTGCAACAGAGAGGAAATGCCCATGTCCACAGCTTGTACACACTGCCCCCTCACT ATCTTGTTATCCAGTGGCATGCCAAAGGAGAACTGAATTAGCTTCTGAGGCTTCTGCTGTAAATCAGAAGTGTATGTTAGTCAAGAGTAAACAAGATGCA CCCAGTATGGTGGGAGGGTTTTGCTGTCAGTAGCTCAAAGTATGGTGTAGAAATGGCCTCCTCCCTCCATCCTGGGAAGTCCCAGTCCCATCCTGGTGTG AGAATCAACCAGGCTTTCCTGCTCCACCTGAGATAACCAACTCCCTCCCGTAATCAGGAAGCCAAATGTCACCTTCCCAAAGAAATTTTATTTTCACGTA GCTGAAGTGCAAAACATAGATGACCATTTTTAATAAGCACAATCAAATTTTTAACCACAGAATGTCTACAAGAATTATAGCTTTAAAAAATACAACCAAT TTTTATATTTCAAAAATATTTGAACTCAAATAAATTAATTTCTTAAAAAGTACACTTCTCATACTATGTGTTTGGCATTGACTCTGGTTAACTCTTGTGC AGCAACACCTACGAGATGCTATCACACACGCGCCAGCTGCACTCCTGCACCAGCCGCTGCAACGACTGCACCTCACGTGGTGGCGGGGACTCACATTCAC AAGACGGGACGGGAACATGCAGTAATAAAATAGCTTTTTAAAAAATAACACATATAAATTCATCACATAGGATTATGCTTTTCACTGCAACCTTGAAGGT GGCATTTATCTCAATCTGTGACTCCAGCTATTTAGTGGCAGACAATCCCGGTGAGGCAGCCTGTGGTCCCACCTTGTAGGTGGCAGCCCCTGCAATCTGG GGTCTAGCCTGTCGGCGTTTGCTTTATTGTGGGTGGAGCAGCTGCTGCAGAATCTGTGAGTTGACTCACCAGTCCCTACAGTTCTGCTACCAAAGTGGTC TTCAATTTAGGGGCCTGACCCCTCCCCAGCTGGAGACAAGCTGCCATCTCCAGGGCTCGCTGTAAACAGCCTCCACTCTTGGGCTGAATAAGCACTCCCA AGAAGCTGGGCCCCTACCAACAGATTAATCGGCTGCAGAGTGCTCATCCCCTCCCCACACCCCCATCCACCCCTGCCTCCTCCTCCTTTCCACTGCTTTA ACCAAACCTTAATGAGGTAAGGCCAGGCTAGGTCAGGGCTCAAAGGAAAATGGGCTCAGGAAAACTTAGTTTGCCCAGCCTTGTCAGCTGAGATTGGTTT TGCTTTCCAGCCAGAAGTAGAAACTTGATGCTTTTTCCTTTTTGCAGTGTTAAAAACCATATCTCCAGTTTTATTATGTTCCATCACAGTTTTGGGGCAA AGCCAGAAAGCTGACTTGTTTGCTTCAAAACACAAACCAAGGCACTGTCTTCTGTGCTTTTCAGGGAGGTGGGAAGGTAAAGGAGACTGCCCACTATAGG GGCTCAGTTCCCCCAAAGGCCTTGCACACCTTCTGATAAACCCCCTGGCTGAGGCAATCCTACTGGCTGTGCAGACAAAGATGTGACAGCAGCACTAGAT GCTGACCCAACAAGCAGTGCTGGGCCTGGGCGGTGGGGCATGTTCAGCACTGCGGGCAGCAGTCAGCACATGCAAGACATTCAGTGAGCAGAGGGCTGCC CTCGGCCTCCAACTGCAGCCGCCCTGGCGCGTGAAATGAAATTGGTGGGCCTTCCTAGGATTGTAAAATGGGACTTGTCATTTGTGTGAGGTTGAAATCT ACTATACTTTGAATATGGCAACTGTTTTCAACCACTGAAGCAAAGAAGGTAAAGGCCAGATGGGGAATGAAAGGTAAGGTACTGTGGTCTATTTCCAAAT TTGGGAGCAATGTGTGTAACTTCAACTGTTGGAGTAAATGAGTTATGGAAAAGCCACAATTTCTAAAATCCCAAGGTTCTACCAAAGGAGTGAGGTTCCT GGGGAAACAGGCATGAGGCAGCAGCTCAGGCTGATGTGTCCTGTGCGATATAAAAAGCTGTGGCCTGATCTCTAAAGTGGGGCTTTTGAAAATCACCAGT CCACAACTACCTGCCTGGCTAGTTTAACCTAGACTGCTTCAGAAGCAAGGGAGGGGGATAATGGACTGGAGAAAAAGCTGACACAAAACTCAAGACAACC AGTTTCAGTACCTGGGCCCTCAAAGCAATAGTGAGGACTGAGAAAGTAGCAAGGGGCACTGATGTCATCTATTTTCTGGGAAGTGTAATTTTTAATGGTT TCATTAAAAACTTACTAACCTTCTACTGGAAGAATACCTCAAATGAAAAATTTTTTTTTTTTGAGACAGTTTTCGCTCTTGTTGCCCAGGGTGGAGTGCA GTGGCGCGATCTCGGCTCACTGTAACCTCTGCCTCCTGGGTTCAAGTGATTCTCCTGCCTCAGCCTCCCGAGTAAGTGGGATTACAGGCACGCACCACCA GACCTGGCTAACTTTCTTTGTATTTTTAGTAGAGATGGGGTTTCACCATGTTGGCCAGGCTGGTCTCCAACTCCTGACCTCAGGTGATCCGCCCACCTCG GCCTCCCAAAGTGTTGGGATTACAGGCGTGAGCCACTGCGCCCGGCCTCAACTGAAAATTCTTGATGAGATTAGTCCCACTGCATAGGTCTCAGCACTGA GGTTTAGAGATGTGAGACTCAGAGTAGGTTTCAGTTCCCCGGAGTGAAGGGGGCATAGGGAGGGGCAGGGCTTCCCAGTGTTAACTGCCTCTGCCCAAAT CATGACTCAGTCCTGGTACCACGTCAAGATCCCTTTGAAAGGAAACATACTTTGCATCAAGTAATCCTTTGAAACTGCCACATTGAAAAGTTAGTTTTTA ACCCAAATATAAATCTGGGAGCTAAACACTAGTCACGAAGCCATCTTTACTTAATGAAGAACCACCTTGAGGGAGACCAGCCCTGCTTGCCACTTACATG GCTGCCCCTGAGACGGTCTGTTCTGTTCATGCCCCAGCAGCCTTTTGCCTTAGGTTTCAGGACCTTCTGAACCACCCTTGAGGGTGTGTGCCAGCAAATA CCTCAAGTGGACTTCTACTTAGGACCACTTGAGGGCACAACTTCAGCTTCTAGAAGGCCAAGTGTGGCTGCAATTCAGGATCTGCCCCAAACTCATCAGC CCTTCCCAAGTTTCCCAGGTGAGAACAGTATGAGCTGTGAATTTCTGTGTTGGTGTGACAGGAATGCCACAGTGGTGGTGCCAGTGATCACTTTCTGACC TTCCTTCCTTCTCAGATAAAATGTTGCACAGTTATGGCCTTGGAGGCCTAAGTAGACAGCAATTAACCAGCCAGTGAGAAAACGAATCAGCATCTAGAAC AGGAGGGGAGGGAACCGTAACAGATAAGGGAGCCCACGATTAGAAGCAGGCTCCCTGCACAGCCTCTGCCTCCAGCGCAAGTAACAGACGTTTCACATTT AAAGGCTGCCAAGGTATTGCTGGCGACTCTCCTGTCTGTGAACATGCGCAACGGGCACAGGTACAGAGAACCAATGAGATGTAGGCAGCACCATCTGCCA CAAAAGGTTCTCCATCGCAGCCCCAGGAGCAAAATCAAACACCTCTGATGGGAGCAAGGACCCTGAACAGAAGCCAACGGCTGGAGCCAACGGGCCATGC CCTTAAAACGAGGACTTGGACAAGGAGGGAACCACTGGAGTGACGTGATGCAAGCCCTCTTCGGTCACTCTTGGCTTCCACCAGGGGTCTCTGCCTCCTT GATGAGAAGAGAAGCTGGCTGGCTCCATGATGACCTCGATGGCGTACTACAGGCCGATGGGCAGGCCGGGCCTCAGCAGCAGGCGCCAACAGAAGCACAC CCAGGGCTGATTCAGGTCCATTTCAGCATAATTTTTGTGGAACAAATTAAAGAAGCAGTAATACCAGAGCTGTCCCCACTTAGGGAATAAAATTTTCAAG GCTGCAAGAAACTCTCTGTCTACAAACCAGTGGCCATTGCCTAAGGAGGCTCGGGGAGGCCAGGGGATCACTATCAGTGGGATAGGTGCTGGCGGAACCA GGGGGTGTCTCCTTCCCAGTTTCAAACTTGTCCTCAGAAGGGGGACTCAACTGGGGACGGCCGTGAGCTGTGGTGACTGAGGTGCACACAGCCTCCCAGA GGCAGAACCTGAAGAGCGCCACGTGGTAGCTCATCCAGGAAATCCGTGCTATTTGAGTTCTCATCGGCTGGGCCAACCACCTGCACCAGCTGACTAAACA GAACCGTTGCTGTCCCAAGCCCACGAGACCCAGTGAGAGG

SHMT1.u.f.24 (forward primer)
SHMT1.u.r.18 (reverse primer)
Single exon skipping

Frame 0

FFFRWSLALLPRLECWSAVAQSRLTASSTSRVH AILLPQPPK*LGLQAPATISG*FFAFLVETGFHR VSQDGLDLLTS*SARLSLPKCWDYRCEPPRPAP TSCFSMLYPVTLLNLLILTVGP*HLDFLGILCC LCVMSVFKFLLS*SLCLLFLFLASLYLE*K*Y*Q AFWSCS*R*PFWFPLCGPVTFVSFCRCFLVPSQ QVVPQQQGSLQPWDSSGTPGSVSHTWLSRLNTP VSILSHPQVLI*DIIPLVMVGGASSSGRTGGRCG WRKQPEPASLTFHR*TQILATSTMTSWRRTHAS STRS*SSQEPAATPETWNMPGYGRLQMRTGRIS WRTWLTSAGWWRLAWCPPHLNTAMW*PPPLTRPC EAAELA*SSTGKE*KVWIPRLAKRFCTTWSLLS ILLCSLACREVPTTTPLLGLLWH*SKL*LWNLK FINTRWWPTAGLCLRP*RSWATK*SQVVLTTI*S LWISVPKAQMVEGLRRC*KPVLLPATRTPVQVT EALCGPVDCGWGPQH*RPVDFWKKTSKK*PTLF TEG*S*PCRSRATLVSEPP*KSSRRDWQGISTRR PCRLSGRRLRASPLSSLCLACLTSKGAGPLWTH LAPQRKLPAGGPPPERWMSCSKGELLTLGPFEG VSFGLFSCFLHKSKFV*VSLLVILGQVIKGFKFE PGFLTAGHNSRKIKYYVATWS*SFRWWCRAKLL ERL*RFTLPGLSSALLQQRGNAHVHSLYTLPPH YLVIQWHAKGELN*LLRLLL*IRSVC*SRVNKMH PVWWEGFAVSSSKYGVEMASSLHPGKSQSHPGV RINQAFLLHLR*PTPSRNQEAKCHLPKEILFSR S*SAKHR*PFLISTIKFLTTECLQEL*L*KIQPI FIFQKYLNSNKLIS*KVHFSYYVFGIDSG*LLC SNTYEMLSHTRQLHSCTSRCNDCTSRGGGDSHS QDGTGTCSNKIAF*KITHINSSHRIMLFTATLKV AFISICDSSYLVADNPGEAACGPTL*VAAPAIW GLACRRLLYCGWSSCCRICELTHQSLQFCYQSG LQFRGLTPPQLETSCHLQGSL*TASTLGLNKHSQ EAGPLPTD*SAAECSSPPHTPIHPCLLLLSTAL TKP**GKARLGQGSKENGLRKT*FAQPCQLRLV LLSSQK*KLDAFSFLQC*KPYLQFYYVPSQFWGK ARKLTCLLQNTNQGTVFCAFQGGGKVKETAHYR GSVPPKALHTF**TPWLRQSYWLCRQRCDSSTR C*PNKQCWAWAVGHVQHCGQQSAHARHSVSRGLP SASNCSRPGA*NEIGGPS*DCKMGLVICVRLKS TIL*IWQLFSTTEAKKVKARWGMKGKVLWSISK FGSNVCNFNCWSK*VMEKPQFLKSQGSTKGVRFL GKQA*GSSSG*CVLCDIKSCGLISKVGLLKITS PQLPAWLV*PRLLQKQGRGIMDWRKS*HKTQDN QFQYLGPQSNSED*ESSKGH*CHLFSGKCNF*WF H*KLTNLLLEEYLK*KIFFF*DSFRSCCPGWSA VARSRLTVTSASWVQVILLPQPPE*VGLQARTT RPG*LSLYF**RWGFTMLARLVSNS*PQVIRPPR PPKVLGLQA*ATAPGLN*KFLMRLVPLHRSQH* GLEM*DSE*VSVPRSEGGIGRGRASQC*LPLPK S*LSPGTTSRSL*KETYFASSNPLKLPH*KVSF* PKYKSGS*TLVTKPSLLNEEPP*GRPALLATYM AAPETVCSVHAPAAFCLRFQDLLNHP*GCVPAN TSSGLLLRTT*GHNFSF*KAKCGCNSGSAPNSSA LPKFPR*EQYEL*ISVLV*QECHSGGASDHFLT FLPSQIKCCTVMALEA*VDSN*PASEKTNQHLE QEGREP*QIREPTIRSRLPAQPLPPAQVTDVSHL KAAKVLLATLLSVNMRNGHRYREPMRCRQHHLP QKVLHRSPRSKIKHL*WEQGP*TEANGWSQRAM PLKRGLGQGGNHWSDVMQALFGHSWLPPGVSASL MRREAGWLHDDLDGVLQADGQAGPQQQAPTEAH PGLIQVHFSIIFVEQIKEAVIPELSPLRE*NFQ GCKKLSVYKPVAIA*GGSGRPGDHYQWDRCWRNQ GVSPSQFQTCPQKGDSTGDGRELW*LRCTQPPR GRT*RAPRGSSSRKSVLFEFSSAGPTTCTS*LN RTVAVPSPRDPVR

Frame 1

FFSDGVSLCCPGWSAGVQWHNLGSLQAPPPGFT PFSCLSLPSSWDYRRPPPYLANFLHF**RRGFT VLARMVSIS*PHDLPVSASQSAGITGVSHRAQPL LLVFQCCIQ*PC*ICLF*QLAPSILIF*VYCVV CV*CQFLNFFFPNRYAFCFFFLPHYTWNRSSIS RHFGLVPEGNHFGFHCVVPSHLFHSAGAFWFLLS R*CPSSRAHYNPGTPQGLLGL*AILG*AG*THQ YPSCLTLKSSSRTSFLLSWWVEPVQVGGQEAGV VGGSSLNLPP*HSTGEPRYWLHQL*PAGGERTPL PPEADHRRNQLLLPKPGICPATEDCR*ERGVSH GGHGSHQRAGGGWRGALPI*TLPCGDHHHSQDP ARLPSWHDLLQERSEKCGSQDWQRDSVQPGVSYQ FCCVPWPAGRSPQPRHCWGCCGTEASYDSGI*S LSTPGGGQLQGSV*GPDGAGLQNSHRWF*QPFD PCGSPFQRHRWWKG*EGARSLFYCLQQEHLSR*Q KRSAAQWTAAGDPSTDVPWTFGKRLPKSSPLYS QRDRADPADPERHWCQSHPERVQGETGRG*VPG GRAGSPGGG*ELRLSLPSAWPA*LLKERAHSGPT WRHRGSCLPEDPHLRDG*AAPKGNC*HSGPLRG FLLDFFHVFFTNQNLFKSHC**FWDRLLKDLNL NLAFSQLDIILGK*NTMSPLGHNHLDGGVGQSC* KDCSVLLSLGFPPPCCNREEMPMSTACTHCPLT ILLSSGMPKEN*ISF*GFCCKSEVYVSQE*TRC TQYGGRVLLSVAQSMV*KWPPPSILGSPSPILV* ESTRLSCST*DNQLPPVIRKPNVTFPKKFYFHV AEVQNIDDHF**AQSNF*PQNVYKNYSFKKYNQ FLYFKNI*TQIN*FLKKYTSHTMCLALTLVNSCA ATPTRCYHTRASCTPAPAAATTAPHVVAGTHIH KTGREHAVIK*LFKK*HI*IHHIGLCFSLQP*R WHLSQSVTPAI*WQTIPVRQPVVPPCRWQPLQSG V*PVGVCFIVGGAAAAESVS*LTSPYSSATKVV FNLGA*PLPSWRQAAISRARCKQPPLLG*ISTP KKLGPYQQINRLQSAHPLPTPPSTPASSSFPLL* PNLNEVRPG*VRAQRKMGSGKLSLPSLVS*DWF CFPARSRNLMLFPFCSVKNHISSFIMFHHSFGA KPES*LVCFKTQTKALSSVLFREVGR*RRLPTIG AQFPQRPCTPSDKPPG*GNPTGCADKDVTAALD ADPTSSAGPGRWGMFSTAGSSQHMQDIQ*AEGC PRPPTAAALAREMKLVGLPRIVKWDLSFV*G*NL LYFEYGNCFQPLKQRR*RPDGE*KVRYCGLFPN LGAMCVTSTVGVNELWKSHNF*NPKVLPKE*GS WGNRHEAAAQADVSCAI*KAVA*SLKWGF*KSPV HNYLPG*FNLDCFRSKGGG*WTGEKADTKLKTT SFSTWALKAIVRTEKVARGTDVIYFLGSVIFNG FIKNLLTFYWKNTSNEKFFFFETVFALVAQGGVQ WRDLGSL*PLPPGFK*FSCLSLPSKWDYRHAPP DLANFLCIFSRDGVSPCWPGWSPTPDLR*SAHL GLPKCWDYRREPLRPASTENS**D*SHCIGLSTE V*RCETQSRFQFPGVKGA*GGAGLPSVNCLCPN HDSVLVPRQDPFERKHTLHQVIL*NCHIEKLVF NPNINLGAKH*SRSHLYLMKNHLEGDQPCLPLTW LPLRRSVLFMPQQPFALGFRTF*TTLEGVCQQI PQVDFYLGPLEGTTSASRRPSVAAIQDLPQTHQ PFPSFPGENSMSCEFLCWCDRNATVVVPVITF*P SFLLR*NVAQLWPWRPK*TAINQPVRKRISI*N RRGGNRNR*GSPRLEAGSLHSLCLQRK*QTFHI *RLPRYCWRLSCL*TCATGTGTENQ*DVGSTICH KRFSIAAPGAKSNTSDGSKDPEQKPTAGANGPC P*NEDLDKEGTTGVT*CKPSSVTLGFHQGSLPP **EEKLAGSMMTSMAYYRPMGRPGLSSRRQQKHT QG*FRSISA*FLWNKLKKQ*YQSCPHLGNKIFK AARNSLSTNQWPLPKEARGGQGITISGIGAGGT RGCLLPSFKLVLRRGTQLGTAVSCGD*GAHSLPE AEPEERHVVAHPGNPCYLSSHRLGQPPAPAD*T EPLLSQAHETQ*E

Frame 2

FFQMESRSVAQAGVLECSGTISAHCKLHLPGSR HSPASASQVAGITGARHHIWLIFCIFSRDGVSP C*PGWSRSPDLMICPSQPPKVLGLQV*ATAPSP YFLFFNVVSSDLAKFAYSNSWPLAS*FSRYIVLS VCNVSF*ISSFLIVMPFVSFSCLIILGIEVVLA GILVLFLKVTILVSIVWSRHICFILQVLFGSFS AGSAPAAGLTTTLGLLRDSWVCEPYLVEQAEHTS IHPVSPSSPHLGHHSSCHGGWSQFKWEDRRQVW LEEAA*TCLPDIPQVNPDTGYINYDQLEENARL FHPKLIIAGTSCYSRNLEYARLRKIADENGAYLM ADMAHISGLVAAGVVPSPFEHCHVVTTTTHKTL RGCRAGMIFYRKGVKSVDPKTGKEILYNLESLI NSAVFPGLQGGPHNHAIAGVAVALKQAMTLEFKV YQHQVVANCRALSEALTELGYKIVTGGSDNHLI LVDLRSKGTDGGRAEKVLEACSIACNKNTCPGD RSALRPSGLRLGTPALTSRGLLEKDFQKVAHFIH RGIELTLQIQSDTGVRATLKEFKERLAGDKYQA AVQALREEVESFASLFPLPGLPDF*RSGPTLDP PGATEEAACRRTPT*EMDELLQRGTVDTRAL*GG FFWTFFMFSSQIKICLSLIVSNSGTGY*RI*I* TWLSHSWT*F*ENKILCRHLVIII*MVV*GKAV RKIVAFYSPWAFLRLAATERKCPCPQLVHTAPSL SCYPVACQRRTELASEASAVNQKCMLVKSKQDA PSMVGGFCCQ*LKVWCRNGLLPPSWEVPVPSWC ENQPGFPAPPEITNSLP*SGSQMSPSQRNFIFT* LKCKT*MTIFNKHNQIFNHRMSTRIIALKNTTN FYISKIFELK*INFLKSTLLILCVWH*LWLTLV QQHLRDAITHAPAALLHQPLQRLHLTWWRGLTFT RRDGNMQ**NSFLKNNTYKFIT*DYAFHCNLEG GIYLNL*LQLFSGRQSR*GSLWSHLVGGSPCNL GSSLSAFALLWVEQLLQNL*VDSPVPTVLLPKWS SI*GPDPSPAGDKLPSPGLAVNSLHSWAE*ALP RSWAPTNRLIGCRVLIPSPHPHPPLPPPPFHCF NQTLMR*GQARSGLKGKWAQENLVCPALSAEIGF AFQPEVET*CFFLFAVLKTISPVLLCSITVLGQ SQKADLFASKHKPRHCLLCFSGRWEGKGDCPL* GLSSPKGLAHLLINPLAEAILLAVQTKM*QQH*M LTQQAVLGLGGGACSALRAAVSTCKTFSEQRAA LGLQLQPPWRVK*NWWAFLGL*NGTCHLCEVEI YYTLNMATVFNH*SKEGKGQMGNER*GTVVYFQI WEQCV*LQLLE*MSYGKATISKIPRFYQRSEVP GETGMRQQLRLMCPVRYKKLWPDL*SGAFENHQ STTTCLASLT*TASEAREGDNGLEKKLTQNSRQP VSVPGPSKQ**GLRK*QGALMSSIFWEV*FLMV SLKTY*PSTGRIPQMKNFFFLRQFSLLLPRVEC SGAISAHCNLCLLGSSDSPASASRVSGITGTHHQ TWLTFFVFLVEMGFHHVGQAGLQLLTSGDPPTS ASQSVGITGVSHCARPQLKILDEISPTA*VSAL RFRDVRLRVGFSSPE*RGHREGQGFPVLTASAQI MTQSWYHVKIPLKGNILCIK*SFETATLKS*FL TQI*IWELNTSHEAIFT**RTTLRETSPACHLH GCP*DGLFCSCPSSLLP*VSGPSEPPLRVCASKY LKWTST*DHLRAQLQLLEGQVWLQFRICPKLIS PSQVSQVRTV*AVNFCVGVTGMPQWWCQ*SLSD LPSFSDKMLHSYGLGGLSRQQLTSQ*ENESASRT GGEGTVTDKGAHD*KQAPCTASASSASNRRFTF KGCQGIAGDSPVCEHAQRAQVQRTNEM*AAPSA TKGSPSQPQEQNQTPLMGARTLNRSQRLEPTGHA LKTRTWTRREPLE*RDASPLRSLLASTRGLCLL DEKRSWLAP**PRWRTTGRWAGRASAAGANRST PRADSGPFQHNFCGTN*RSSNTRAVPT*GIKFSR LQETLCLQTSGHCLRRLGEARGSLSVG*VLAEP GGVSFPVSNLSSEGGLNWGRP*AVVTEVHTASQ RQNLKSATW*LIQEIRAI*VLIGWANHLHQLTKQ NRCCPKPTRPSER

Sequence SHMT1.cAug05

CGGGGGCGGGGCCAAGCCCAAGCCTGGCGCGCAGAGTGCACCTTCCTGAGCTCGAGCGGTCCAGCGCCAAGTTCGGGGTTTGGGGTTGGAGCGGCTGGTC ACGTGGCTGGCCCGCGGCGGTGCGCGGGGCGTTGGGTCAGCGGGTCTGGGACTGGTGGCACCGGCGGCGGCGTAGGACGGAGGCGTCGCTAGGCAGCTTC GAACCAGTGCAATGACGATGCCAGTCAACGGGGCCCACAAGGATGCTGACCTGTGGTCCTCACATGACAAGATGCTGGCACAACCCCTCAAAGACAGTGA TGTTGAGGTTTACAACATCATTAAGAAGGAGAGTAACCGGCAGAGGGTTGGATTGGAGCTGATTGCCTCGGAGAATTTCGCCAGCCGAGCAGTTTTGGAG GCCCTAGGCTCTTGCTTAAATAACAAATACTCTGAGGGGTACCCGGGCCAGAGATACTATGGCGGGACTGAGTTTATTGATGAACTGGAGACCCTCTGTC AGAAGCGAGCCCTGCAGGCCTATAAGCTGGACCCACAGTGCTGGGGGGTCAACGTCCAGCCCTACTCAGGCTCCCCTGCAAACTTTGCTGTGTACACTGC CCTGGTGGAACCCCATGGGCGCATCATGGGCCTGGACCTTCCGGATGGGGGCCACCTGACCCATGGGTTCATGACAGACAAGAAGAAAATCTCTGCCACG TCCATCTTCTTTGAATCTATGCCCTACAAGGTGAACCCAGATACTGGCTACATCAACTATGACCAGCTGGAGGAGAACGCACGCCTCTTCCACCCGAAGC TGATCATCGCAGGAACCAGCTGCTACTCCCGAAACCTGGAATATGCCCGGCTACGGAAGATTGCAGATGAGAACGGGGCGTATCTCATGGCGGACATGGC TCACATCAGCGGGCTGGTGGCGGCTGGCGTGGTGCCCTCCCCATTTGAACACTGCCATGTGGTGACCACCACCACTCACAAGACCCTGCGAGGCTGCCGA GCTGGCATGATCTTCTACAGGAAAGGAGTGAAAAGTGTGGATCCCAAGACTGGCAAAGAGATTCTGTACAACCTGGAGTCTCTTATCAATTCTGCTGTGT TCCCTGGCCTGCAGGGAGGTCCCCACAACCACGCCATTGCTGGGGTTGCTGTGGCACTGAAGCAAGCTATGACTCTGGAATTTAAAGTTTATCAACACCA GGTGGTGGCCAACTGCAGGGCTCTGTCTGAGGCCCTGACGGAGCTGGGCTACAAAATAGTCACAGGTGGTTCTGACAACCATTTGATCCTTGTGGATCTC CGTTCCAAAGGCACAGATGGTGGAAGGGCTGAGAAGGTGCTAGAAGCCTGTTCTATTGCCTGCAACAAGAACACCTGTCCAGGGATAGAGCTGACCCTGC AGATCCAGAGCGACACTGGTGTCAGAGCCACCCTGAAAGAGTTCAAGGAGAGACTGGCAGGGGATAAGTACCAGGCGGCCGTGCAGGCTCTCCGGGAGGA GGTTGAGAGCTTCGCCTCTCTCTTCCCTCTGCCTGGCCTGCCTGACTTCTAAAGGAGCGGGCCCACTCTGGACCCACCTGGCGCCACAGAGGAAGCTGCC TGCCGGAGGACCCCCACCTGAGAGATGGATGAGCTGCTCCAAAGGGGAACTGTTGACACTCGGGCCCTTTGAGGGGGTTTCTTTTGGACTTTTTTCATGT TTTCTTCACAAATCAAAATTTGTTTAAGTCTCATTGTTAGTAATTCTGGGACAGGTTATTAAAGGATTTAAATTTGAACCTGGCTTTCTCACAGCTGGAC ATAATTCTAGGAAAATAAAATACTATGTCGCCACTTGGTCATAATCATTTAGATGGTGGTGTAGGGCAAAGCTGTTAGAAAGATTGTAGCGTTTTACTCT CCCTGGGCTTTCCTCCGCCTTGCTGCAACAGAGAGGAAATGCCCATGTCCACAGCTTGTACACACTGCCCCCTCACTATCTTGTTATCCAGTGGCATGCC AAAGGAGAACTGAATTAGCTTCTGAGGCTTCTGCTGTAAATCAGAAGTGTATGTTAGTCAAGAGTAAACAAGATGCACCCAGTATGGTGGGAGGGTTTTG CTGTCAGTAGCTCAAAGTATGGTGTAGAAATGGCCTCCTCCCTCCATCCTGGGAAGTCCCAGTCCCATCCTGGTGTGAGAATCAACCAGGCTTTCCTGCT CCACCTGAGATAACCAACTCCCTCCCGTAATCAGGAAGCCAAATGTCACCTTCCCAAAGAAATTTTATTTTCACGTAGCTGAAGTGCAAAACATAGATGA CCATTTTTAATAAGCACAATCAAATTTTTAACCACAGAATGTCTACAAGAATTATAGCTTTAAAAAATACAACCAATTTTTATATTTCAAAAATATTTGA ACTCAAATAAATTAATTTCTTAAAAAGTACACTTCTCATACTATGTGTTTGGCATTGACTCTGGTTAACTCTTGTGCAGCAACACCTACGAGATGCTATC ACACACGCGCCAGCTGCACTCCTGCACCAGCCGCTGCAACGACTGCACCTCACGTGGTGGCGGGGACTCACATTCACAAGACGGGACGGGAACATGCAGT AATAAAATAGCTTTTTAAAAAATAACACATATAAATTCATCACATAGGATTATGCTTTTCACTGCAACCTTGAAGGTGGCATTTATCTCAATCTGTGACT CCAGCTATTTAGTGGCAGACAATCCCGGTGAGGCAGCCTGTGGTCCCACCTTGTAGGTGGCAGCCCCTGCAATCTGGGGTCTAGCCTGTCGGCGTTTGCT TTATTGTGGGTGGAGCAGCTGCTGCAGAATCTGTGAGTTGACTCACCAGTCCCTACAGTTCTGCTACCAAAGTGGTCTTCAATTTAGGGGCCTGACCCCT CCCCAGCTGGAGACAAGCTGCCATCTCCAGGGCTCGCTGTAAACAGCCTCCACTCTTGGGCTGAATAAGCACTCCCAAGAAGCTGGGCCCCTACCAACAG ATTAATCGGCTGCAGAGTGCTCATCCCCTCCCCACACCCCCATCCACCCCTGCCTCCTCCTCCTTTCCACTGCTTTAACCAAACCTTAATGAGGTAAGGC CAGGCTAGGTCAGGGCTCAAAGGAAAATGGGCTCAGGAAAACTTAGTTTGCCCAGCCTTGTCAGCTGAGATTGGTTTTGCTTTCCAGCCAGAAGTAGAAA CTTGATGCTTTTTCCTTTTTGCAGTGTTAAAAACCATATCTCCAGTTTTATTATGTTCCATCACAGTTTTGGGGCAAAGCCAGAAAGCTGACTTGTTTGC TTCAAAACACAAACCAAGGCACTGTCTTCTGTGCTTTTCAGGGAGGTGGGAAGGTAAAGGAGACTGCCCACTATAGGGGCTCAGTTCCCCCAAAGGCCTT GCACACCTTCTGATAAACCCCCTGGCTGAGGCAATCCTACTGGCTGTGCAGACAAAGATGTGACAGCAGCACTAGATGCTGACCCAACAAGCAGTGCTGG GCCTGGGCGGTGGGGCATGTTCAGCACTGCGGGCAGCAGTCAGCACATGCAAGACATTCAGTGAGCAGAGGGCTGCCCTCGGCCTCCAACTGCAGCCGCC CTGGCGCGTGAAATGAAATTGGTGGGCCTTCCTAGGATTGTAAAATGGGACTTGTCATTTGTGTGAGGTTGAAATCTACTATACTTTGAATATGGCAACT GTTTTCAACCACTGAAGCAAAGAAGGTAAAGGCCAGATGGGGAATGAAAGGTAAGGTACTGTGGTCTATTTCCAAATTTGGGAGCAATGTGTGTAACTTC AACTGTTGGAGTAAATGAGTTATGGAAAAGCCACAATTTCTAAAATCCCAAGGTTCTACCAAAGGAGTGAGGTTCCTGGGGAAACAGGCATGAGGCAGCA GCTCAGGCTGATGTGTCCTGTGCGATATAAAAAGCTGTGGCCTGATCTCTAAAGTGGGGCTTTTGAAAATCACCAGTCCACAACTACCTGCCTGGCTAGT TTAACCTAGACTGCTTCAGAAGCAAGGGAGGGGGATAATGGACTGGAGAAAAAGCTGACACAAAACTCAAGACAACCAGTTTCAGTACCTGGGCCCTCAA AGCAATAGTGAGGACTGAGAAAGTAGCAAGGGGCACTGATGTCATCTATTTTCTGGGAAGTGTAATTTTTAATGGTTTCATTAAAAACTTACTAACCTTC TACTGGAAGAATACCTCAAATGAAAAATTTTTTTTTTTTGAGACAGTTTTCGCTCTTGTTGCCCAGGGTGGAGTGCAGTGGCGCGATCTCGGCTCACTGT AACCTCTGCCTCCTGGGTTCAAGTGATTCTCCTGCCTCAGCCTCCCGAGTAAGTGGGATTACAGGCACGCACCACCAGACCTGGCTAACTTTCTTTGTAT TTTTAGTAGAGATGGGGTTTCACCATGTTGGCCAGGCTGGTCTCCAACTCCTGACCTCAGGTGATCCGCCCACCTCGGCCTCCCAAAGTGTTGGGATTAC AGGCGTGAGCCACTGCGCCCGGCCTCAACTGAAAATTCTTGATGAGATTAGTCCCACTGCATAGGTCTCAGCACTGAGGTTTAGAGATGTGAGACTCAGA GTAGGTTTCAGTTCCCCGGAGTGAAGGGGGCATAGGGAGGGGCAGGGCTTCCCAGTGTTAACTGCCTCTGCCCAAATCATGACTCAGTCCTGGTACCACG TCAAGATCCCTTTGAAAGGAAACATACTTTGCATCAAGTAATCCTTTGAAACTGCCACATTGAAAAGTTAGTTTTTAACCCAAATATAAATCTGGGAGCT AAACACTAGTCACGAAGCCATCTTTACTTAATGAAGAACCACCTTGAGGGAGACCAGCCCTGCTTGCCACTTACATGGCTGCCCCTGAGACGGTCTGTTC TGTTCATGCCCCAGCAGCCTTTTGCCTTAGGTTTCAGGACCTTCTGAACCACCCTTGAGGGTGTGTGCCAGCAAATACCTCAAGTGGACTTCTACTTAGG ACCACTTGAGGGCACAACTTCAGCTTCTAGAAGGCCAAGTGTGGCTGCAATTCAGGATCTGCCCCAAACTCATCAGCCCTTCCCAAGTTTCCCAGGTGAG AACAGTATGAGCTGTGAATTTCTGTGTTGGTGTGACAGGAATGCCACAGTGGTGGTGCCAGTGATCACTTTCTGACCTTCCTTCCTTCTCAGATAAAATG TTGCACAGTTATGGCCTTGGAGGCCTAAGTAGACAGCAATTAACCAGCCAGTGAGAAAACGAATCAGCATCTAGAACAGGAGGGGAGGGAACCGTAACAG ATAAGGGAGCCCACGATTAGAAGCAGGCTCCCTGCACAGCCTCTGCCTCCAGCGCAAGTAACAGACGTTTCACATTTAAAGGCTGCCAAGGTATTGCTGG CGACTCTCCTGTCTGTGAACATGCGCAACGGGCACAGGTACAGAGAACCAATGAGATGTAGGCAGCACCATCTGCCACAAAAGGTTCTCCATCGCAGCCC CAGGAGCAAAATCAAACACCTCTGATGGGAGCAAGGACCCTGAACAGAAGCCAACGGCTGGAGCCAACGGGCCATGCCCTTAAAACGAGGACTTGGACAA GGAGGGAACCACTGGAGTGACGTGATGCAAGCCCTCTTCGGTCACTCTTGGCTTCCACCAGGGGTCTCTGCCTCCTTGATGAGAAGAGAAGCTGGCTGGC TCCATGATGACCTCGATGGCGTACTACAGGCCGATGGGCAGGCCGGGCCTCAGCAGCAGGCGCCAACAGAAGCACACCCAGGGCTGATTCAGGTCCATTT CAGCATAATTTTTGTGGAACAAATTAAAGAAGCAGTAATACCAGAGCTGTCCCCACTTAGGGAATAAAATTTTCAAGGCTGCAAGAAACTCTCTGTCTAC AAACCAGTGGCCATTGCCTAAGGAGGCTCGGGGAGGCCAGGGGATCACTATCAGTGGGATAGGTGCTGGCGGAACCAGGGGGTGTCTCCTTCCCAGTTTC AAACTTGTCCTCAGAAGGGGGACTCAACTGGGGACGGCCGTGAGCTGTGGTGACTGAGGTGCACACAGCCTCCCAGAGGCAGAACCTGAAGAGCGCCACG TGGTAGCTCATCCAGGAAATCCGTGCTATTTGAGTTCTCATCGGCTGGGCCAACCACCTGCACCAGCTGACTAAACAGAACCGTTGCTGTCCCAAGCCCA CGAGACCCAGTGAGAGG

SHMT1.u.f.24 (forward primer)
SHMT1.u.r.18 (reverse primer)
Single exon skipping

Frame 0

RGRGQAQAWRAECTFLSSSGPAPSSGFGVGAAG HVAGPRRCAGRWVSGSGTGGTGGGVGRRRR*AAS NQCNDDASQRGPQGC*PVVLT*QDAGTTPQRQ* C*GLQHH*EGE*PAEGWIGADCLGEFRQPSSFG GPRLLLK*QIL*GVPGPEILWRD*VY**TGDPLS EASPAGL*AGPTVLGGQRPALLRLPCKLCCVHC PGGTPWAHHGPGPSGWGPPDPWVHDRQEENLCH VHLL*IYALQGEPRYWLHQL*PAGGERTPLPPEA DHRRNQLLLPKPGICPATEDCR*ERGVSHGGHG SHQRAGGGWRGALPI*TLPCGDHHHSQDPARLP SWHDLLQERSEKCGSQDWQRDSVQPGVSYQFCCV PWPAGRSPQPRHCWGCCGTEASYDSGI*SLSTP GGGQLQGSV*GPDGAGLQNSHRWF*QPFDPCGS PFQRHRWWKG*EGARSLFYCLQQEHLSRDRADPA DPERHWCQSHPERVQGETGRG*VPGGRAGSPGG G*ELRLSLPSAWPA*LLKERAHSGPTWRHRGSC LPEDPHLRDG*AAPKGNC*HSGPLRGFLLDFFHV FFTNQNLFKSHC**FWDRLLKDLNLNLAFSQLD IILGK*NTMSPLGHNHLDGGVGQSC*KDCSVLL SLGFPPPCCNREEMPMSTACTHCPLTILLSSGMP KEN*ISF*GFCCKSEVYVSQE*TRCTQYGGRVL LSVAQSMV*KWPPPSILGSPSPILV*ESTRLSC ST*DNQLPPVIRKPNVTFPKKFYFHVAEVQNIDD HF**AQSNF*PQNVYKNYSFKKYNQFLYFKNI* TQIN*FLKKYTSHTMCLALTLVNSCAATPTRCY HTRASCTPAPAAATTAPHVVAGTHIHKTGREHAV IK*LFKK*HI*IHHIGLCFSLQP*RWHLSQSVT PAI*WQTIPVRQPVVPPCRWQPLQSGV*PVGVC FIVGGAAAAESVS*LTSPYSSATKVVFNLGA*PL PSWRQAAISRARCKQPPLLG*ISTPKKLGPYQQ INRLQSAHPLPTPPSTPASSSFPLL*PNLNEVR PG*VRAQRKMGSGKLSLPSLVS*DWFCFPARSRN LMLFPFCSVKNHISSFIMFHHSFGAKPES*LVC FKTQTKALSSVLFREVGR*RRLPTIGAQFPQRP CTPSDKPPG*GNPTGCADKDVTAALDADPTSSAG PGRWGMFSTAGSSQHMQDIQ*AEGCPRPPTAAA LAREMKLVGLPRIVKWDLSFV*G*NLLYFEYGN CFQPLKQRR*RPDGE*KVRYCGLFPNLGAMCVTS TVGVNELWKSHNF*NPKVLPKE*GSWGNRHEAA AQADVSCAI*KAVA*SLKWGF*KSPVHNYLPG* FNLDCFRSKGGG*WTGEKADTKLKTTSFSTWALK AIVRTEKVARGTDVIYFLGSVIFNGFIKNLLTF YWKNTSNEKFFFFETVFALVAQGGVQWRDLGSL *PLPPGFK*FSCLSLPSKWDYRHAPPDLANFLCI FSRDGVSPCWPGWSPTPDLR*SAHLGLPKCWDY RREPLRPASTENS**D*SHCIGLSTEV*RCETQ SRFQFPGVKGA*GGAGLPSVNCLCPNHDSVLVPR QDPFERKHTLHQVIL*NCHIEKLVFNPNINLGA KH*SRSHLYLMKNHLEGDQPCLPLTWLPLRRSV LFMPQQPFALGFRTF*TTLEGVCQQIPQVDFYLG PLEGTTSASRRPSVAAIQDLPQTHQPFPSFPGE NSMSCEFLCWCDRNATVVVPVITF*PSFLLR*N VAQLWPWRPK*TAINQPVRKRISI*NRRGGNRNR *GSPRLEAGSLHSLCLQRK*QTFHI*RLPRYCW RLSCL*TCATGTGTENQ*DVGSTICHKRFSIAA PGAKSNTSDGSKDPEQKPTAGANGPCP*NEDLDK EGTTGVT*CKPSSVTLGFHQGSLPP**EEKLAG SMMTSMAYYRPMGRPGLSSRRQQKHTQG*FRSI SA*FLWNKLKKQ*YQSCPHLGNKIFKAARNSLST NQWPLPKEARGGQGITISGIGAGGTRGCLLPSF KLVLRRGTQLGTAVSCGD*GAHSLPEAEPEERH VVAHPGNPCYLSSHRLGQPPAPAD*TEPLLSQAH ETQ*E

Frame 1

GGGAKPKPGAQSAPS*ARAVQRQVRGLGLERLV TWLARGGARGVGSAGLGLVAPAAA*DGGVARQL RTSAMTMPVNGAHKDADLWSSHDKMLAQPLKDSD VEVYNIIKKESNRQRVGLELIASENFASRAVLE ALGSCLNNKYSEGYPGQRYYGGTEFIDELETLC QKRALQAYKLDPQCWGVNVQPYSGSPANFAVYTA LVEPHGRIMGLDLPDGGHLTHGFMTDKKKISAT SIFFESMPYKVNPDTGYINYDQLEENARLFHPK LIIAGTSCYSRNLEYARLRKIADENGAYLMADMA HISGLVAAGVVPSPFEHCHVVTTTTHKTLRGCR AGMIFYRKGVKSVDPKTGKEILYNLESLINSAV FPGLQGGPHNHAIAGVAVALKQAMTLEFKVYQHQ VVANCRALSEALTELGYKIVTGGSDNHLILVDL RSKGTDGGRAEKVLEACSIACNKNTCPGIELTL QIQSDTGVRATLKEFKERLAGDKYQAAVQALREE VESFASLFPLPGLPDF*RSGPTLDPPGATEEAA CRRTPT*EMDELLQRGTVDTRAL*GGFFWTFFM FSSQIKICLSLIVSNSGTGY*RI*I*TWLSHSWT *F*ENKILCRHLVIII*MVV*GKAVRKIVAFYS PWAFLRLAATERKCPCPQLVHTAPSLSCYPVAC QRRTELASEASAVNQKCMLVKSKQDAPSMVGGFC CQ*LKVWCRNGLLPPSWEVPVPSWCENQPGFPA PPEITNSLP*SGSQMSPSQRNFIFT*LKCKT*M TIFNKHNQIFNHRMSTRIIALKNTTNFYISKIFE LK*INFLKSTLLILCVWH*LWLTLVQQHLRDAI THAPAALLHQPLQRLHLTWWRGLTFTRRDGNMQ **NSFLKNNTYKFIT*DYAFHCNLEGGIYLNL*L QLFSGRQSR*GSLWSHLVGGSPCNLGSSLSAFA LLWVEQLLQNL*VDSPVPTVLLPKWSSI*GPDP SPAGDKLPSPGLAVNSLHSWAE*ALPRSWAPTNR LIGCRVLIPSPHPHPPLPPPPFHCFNQTLMR*G QARSGLKGKWAQENLVCPALSAEIGFAFQPEVE T*CFFLFAVLKTISPVLLCSITVLGQSQKADLFA SKHKPRHCLLCFSGRWEGKGDCPL*GLSSPKGL AHLLINPLAEAILLAVQTKM*QQH*MLTQQAVL GLGGGACSALRAAVSTCKTFSEQRAALGLQLQPP WRVK*NWWAFLGL*NGTCHLCEVEIYYTLNMAT VFNH*SKEGKGQMGNER*GTVVYFQIWEQCV*L QLLE*MSYGKATISKIPRFYQRSEVPGETGMRQQ LRLMCPVRYKKLWPDL*SGAFENHQSTTTCLAS LT*TASEAREGDNGLEKKLTQNSRQPVSVPGPS KQ**GLRK*QGALMSSIFWEV*FLMVSLKTY*PS TGRIPQMKNFFFLRQFSLLLPRVECSGAISAHC NLCLLGSSDSPASASRVSGITGTHHQTWLTFFV FLVEMGFHHVGQAGLQLLTSGDPPTSASQSVGIT GVSHCARPQLKILDEISPTA*VSALRFRDVRLR VGFSSPE*RGHREGQGFPVLTASAQIMTQSWYH VKIPLKGNILCIK*SFETATLKS*FLTQI*IWEL NTSHEAIFT**RTTLRETSPACHLHGCP*DGLF CSCPSSLLP*VSGPSEPPLRVCASKYLKWTST* DHLRAQLQLLEGQVWLQFRICPKLISPSQVSQVR TV*AVNFCVGVTGMPQWWCQ*SLSDLPSFSDKM LHSYGLGGLSRQQLTSQ*ENESASRTGGEGTVT DKGAHD*KQAPCTASASSASNRRFTFKGCQGIAG DSPVCEHAQRAQVQRTNEM*AAPSATKGSPSQP QEQNQTPLMGARTLNRSQRLEPTGHALKTRTWT RREPLE*RDASPLRSLLASTRGLCLLDEKRSWLA P**PRWRTTGRWAGRASAAGANRSTPRADSGPF QHNFCGTN*RSSNTRAVPT*GIKFSRLQETLCL QTSGHCLRRLGEARGSLSVG*VLAEPGGVSFPVS NLSSEGGLNWGRP*AVVTEVHTASQRQNLKSAT W*LIQEIRAI*VLIGWANHLHQLTKQNRCCPKP TRPSER

Frame 2

GAGPSPSLARRVHLPELERSSAKFGVWGWSGWS RGWPAAVRGALGQRVWDWWHRRRRRTEASLGSF EPVQ*RCQSTGPTRMLTCGPHMTRCWHNPSKTV MLRFTTSLRRRVTGRGLDWS*LPRRISPAEQFWR P*ALA*ITNTLRGTRARDTMAGLSLLMNWRPSV RSEPCRPISWTHSAGGSTSSPTQAPLQTLLCTL PWWNPMGASWAWTFRMGAT*PMGS*QTRRKSLPR PSSLNLCPTR*TQILATSTMTSWRRTHASSTRS *SSQEPAATPETWNMPGYGRLQMRTGRISWRTW LTSAGWWRLAWCPPHLNTAMW*PPPLTRPCEAAE LA*SSTGKE*KVWIPRLAKRFCTTWSLLSILLC SLACREVPTTTPLLGLLWH*SKL*LWNLKFINT RWWPTAGLCLRP*RSWATK*SQVVLTTI*SLWIS VPKAQMVEGLRRC*KPVLLPATRTPVQG*S*PC RSRATLVSEPP*KSSRRDWQGISTRRPCRLSGR RLRASPLSSLCLACLTSKGAGPLWTHLAPQRKLP AGGPPPERWMSCSKGELLTLGPFEGVSFGLFSC FLHKSKFV*VSLLVILGQVIKGFKFEPGFLTAG HNSRKIKYYVATWS*SFRWWCRAKLLERL*RFTL PGLSSALLQQRGNAHVHSLYTLPPHYLVIQWHA KGELN*LLRLLL*IRSVC*SRVNKMHPVWWEGF AVSSSKYGVEMASSLHPGKSQSHPGVRINQAFLL HLR*PTPSRNQEAKCHLPKEILFSRS*SAKHR* PFLISTIKFLTTECLQEL*L*KIQPIFIFQKYL NSNKLIS*KVHFSYYVFGIDSG*LLCSNTYEMLS HTRQLHSCTSRCNDCTSRGGGDSHSQDGTGTCS NKIAF*KITHINSSHRIMLFTATLKVAFISICD SSYLVADNPGEAACGPTL*VAAPAIWGLACRRLL YCGWSSCCRICELTHQSLQFCYQSGLQFRGLTP PQLETSCHLQGSL*TASTLGLNKHSQEAGPLPT D*SAAECSSPPHTPIHPCLLLLSTALTKP**GKA RLGQGSKENGLRKT*FAQPCQLRLVLLSSQK*K LDAFSFLQC*KPYLQFYYVPSQFWGKARKLTCL LQNTNQGTVFCAFQGGGKVKETAHYRGSVPPKAL HTF**TPWLRQSYWLCRQRCDSSTRC*PNKQCW AWAVGHVQHCGQQSAHARHSVSRGLPSASNCSR PGA*NEIGGPS*DCKMGLVICVRLKSTIL*IWQL FSTTEAKKVKARWGMKGKVLWSISKFGSNVCNF NCWSK*VMEKPQFLKSQGSTKGVRFLGKQA*GS SSG*CVLCDIKSCGLISKVGLLKITSPQLPAWLV *PRLLQKQGRGIMDWRKS*HKTQDNQFQYLGPQ SNSED*ESSKGH*CHLFSGKCNF*WFH*KLTNL LLEEYLK*KIFFF*DSFRSCCPGWSAVARSRLTV TSASWVQVILLPQPPE*VGLQARTTRPG*LSLY F**RWGFTMLARLVSNS*PQVIRPPRPPKVLGL QA*ATAPGLN*KFLMRLVPLHRSQH*GLEM*DSE *VSVPRSEGGIGRGRASQC*LPLPKS*LSPGTT SRSL*KETYFASSNPLKLPH*KVSF*PKYKSGS *TLVTKPSLLNEEPP*GRPALLATYMAAPETVCS VHAPAAFCLRFQDLLNHP*GCVPANTSSGLLLR TT*GHNFSF*KAKCGCNSGSAPNSSALPKFPR* EQYEL*ISVLV*QECHSGGASDHFLTFLPSQIKC CTVMALEA*VDSN*PASEKTNQHLEQEGREP*Q IREPTIRSRLPAQPLPPAQVTDVSHLKAAKVLL ATLLSVNMRNGHRYREPMRCRQHHLPQKVLHRSP RSKIKHL*WEQGP*TEANGWSQRAMPLKRGLGQ GGNHWSDVMQALFGHSWLPPGVSASLMRREAGW LHDDLDGVLQADGQAGPQQQAPTEAHPGLIQVHF SIIFVEQIKEAVIPELSPLRE*NFQGCKKLSVY KPVAIA*GGSGRPGDHYQWDRCWRNQGVSPSQF QTCPQKGDSTGDGRELW*LRCTQPPRGRT*RAPR GSSSRKSVLFEFSSAGPTTCTS*LNRTVAVPSP RDPVR

Sequence SHMT1.bAug05

CGGGGGCGGGGCCAAGCCCAAGCCTGGCGCGCAGAGTGCACCTTCCTGAGCTCGAGCGGTCCAGCGCCAAGTTCGGGGTTTGGGGTTGGAGCGGCTGGTC ACGTGGCTGGCCCGCGGCGGTGCGCGGGGCGTTGGGTCAGCGGGTCTGGGACTGGTGGCACCGGCGGCGGCGTAGGACGGAGGCGTCGCTAGGGTCTTGT TCTCTTGGCCAGGCTGGAGTGCTGTGGGAAAATCTGGGCTCACTGCAGCCTCAACCTCCGGGACTCAAGTGATCATCCTGCCTCAGCCACCCAGAGTAGC TGAGAATACAGGCGTGCGCCACCAGGCTCGGGCAGCTTCGAACCAGTGCAATGACGATGCCAGTCAACGGGGCCCACAAGGATGCTGACCTGTGGTCCTC ACATGACAAGATGCTGGCACAACCCCTCAAAGACAGTGATGTTGAGGTTTACAACATCATTAAGAAGGAGAGTAACCGGCAGAGGGTTGGATTGGAGCTG ATTGCCTCGGAGAATTTCGCCAGCCGAGCAGTTTTGGAGGCCCTAGGCTCTTGCTTAAATAACAAATACTCTGAGGGGTACCCGGGCCAGAGATACTATG GCGGGACTGAGTTTATTGATGAACTGGAGACCCTCTGTCAGAAGCGAGCCCTGCAGGCCTATAAGCTGGACCCACAGTGCTGGGGGGTCAACGTCCAGCC CTACTCAGGCTCCCCTGCAAACTTTGCTGTGTACACTGCCCTGGTGGAACCCCATGGGCGCATCATGGGCCTGGACCTTCCGGATGGGGGCCACCTGACC CATGGGTTCATGACAGACAAGAAGAAAATCTCTGCCACGTCCATCTTCTTTGAATCTATGCCCTACAAGGTGAACCCAGATACTGGCTACATCAACTATG ACCAGCTGGAGGAGAACGCACGCCTCTTCCACCCGAAGCTGATCATCGCAGGAACCAGCTGCTACTCCCGAAACCTGGAATATGCCCGGCTACGGAAGAT TGCAGATGAGAACGGGGCGTATCTCATGGCGGACATGGCTCACATCAGCGGGCTGGTGGCGGCTGGCGTGGTGCCCTCCCCATTTGAACACTGCCATGTG GTGACCACCACCACTCACAAGACCCTGCGAGGCTGCCGAGCTGGCATGATCTTCTACAGGAAAGGAGTGAAAAGTGTGGATCCCAAGACTGGCAAAGAGA TTCTGTACAACCTGGAGTCTCTTATCAATTCTGCTGTGTTCCCTGGCCTGCAGGGAGGTCCCCACAACCACGCCATTGCTGGGGTTGCTGTGGCACTGAA GCAAGCTATGACTCTGGAATTTAAAGTTTATCAACACCAGGTGGTGGCCAACTGCAGGGCTCTGTCTGAGGCCCTGACGGAGCTGGGCTACAAAATAGTC ACAGGTGGTTCTGACAACCATTTGATCCTTGTGGATCTCCGTTCCAAAGGCACAGATGGTGGAAGGGCTGAGAAGGTGCTAGAAGCCTGTTCTATTGCCT GCAACAAGAACACCTGTCCAGGTGACAGAAGCGCTCTGCGGCCCAGTGGACTGCGGCTGGGGACCCCAGCACTGACGTCCCGTGGACTTTTGGAAAAAGA CTTCCACGACACTGGTGTCAGAGCCACCCTGAAAGAGTTCAAGGAGAGACTGGCAGGGGATAAGTACCAGGCGGCCGTGCAGGCTCTCCGGGAGGAGGTT GAGAGCTTCGCCTCTCTCTTCCCTCTGCCTGGCCTGCCTGACTTCTAAAGGAGCGGGCCCACTCTGGACCCACCTGGCGCCACAGAGGAAGCTGCCTGCC GGAGGACCCCCACCTGAGAGATGGATGAGCTGCTCCAAAGGGGAACTGTTGACACTCGGGCCCTTTGAGGGGGTTTCTTTTGGACTTTTTTCATGTTTTC TTCACAAATCAAAATTTGTTTAAGTCTCATTGTTAGTAATTCTGGGACAGGTTATTAAAGGATTTAAATTTGAACCTGGCTTTCTCACAGCTGGACATAA TTCTAGGAAAATAAAATACTATGTCGCCACTTGGTCATAATCATTTAGATGGTGGTGTAGGGCAAAGCTGTTAGAAAGATTGTAGCGTTTTACTCTCCCT GGGCTTTCCTCCGCCTTGCTGCAACAGAGAGGAAATGCCCATGTCCACAGCTTGTACACACTGCCCCCTCACTATCTTGTTATCCAGTGGCATGCCAAAG GAGAACTGAATTAGCTTCTGAGGCTTCTGCTGTAAATCAGAAGTGTATGTTAGTCAAGAGTAAACAAGATGCACCCAGTATGGTGGGAGGGTTTTGCTGT CAGTAGCTCAAAGTATGGTGTAGAAATGGCCTCCTCCCTCCATCCTGGGAAGTCCCAGTCCCATCCTGGTGTGAGAATCAACCAGGCTTTCCTGCTCCAC CTGAGATAACCAACTCCCTCCCGTAATCAGGAAGCCAAATGTCACCTTCCCAAAGAAATTTTATTTTCACGTAGCTGAAGTGCAAAACATAGATGACCAT TTTTAATAAGCACAATCAAATTTTTAACCACAGAATGTCTACAAGAATTATAGCTTTAAAAAATACAACCAATTTTTATATTTCAAAAATATTTGAACTC AAATAAATTAATTTCTTAAAAAGTACACTTCTCATACTATGTGTTTGGCATTGACTCTGGTTAACTCTTGTGCAGCAACACCTACGAGATGCTATCACAC ACGCGCCAGCTGCACTCCTGCACCAGCCGCTGCAACGACTGCACCTCACGTGGTGGCGGGGACTCACATTCACAAGACGGGACGGGAACATGCAGTAATA AAATAGCTTTTTAAAAAATAACACATATAAATTCATCACATAGGATTATGCTTTTCACTGCAACCTTGAAGGTGGCATTTATCTCAATCTGTGACTCCAG CTATTTAGTGGCAGACAATCCCGGTGAGGCAGCCTGTGGTCCCACCTTGTAGGTGGCAGCCCCTGCAATCTGGGGTCTAGCCTGTCGGCGTTTGCTTTAT TGTGGGTGGAGCAGCTGCTGCAGAATCTGTGAGTTGACTCACCAGTCCCTACAGTTCTGCTACCAAAGTGGTCTTCAATTTAGGGGCCTGACCCCTCCCC AGCTGGAGACAAGCTGCCATCTCCAGGGCTCGCTGTAAACAGCCTCCACTCTTGGGCTGAATAAGCACTCCCAAGAAGCTGGGCCCCTACCAACAGATTA ATCGGCTGCAGAGTGCTCATCCCCTCCCCACACCCCCATCCACCCCTGCCTCCTCCTCCTTTCCACTGCTTTAACCAAACCTTAATGAGGTAAGGCCAGG CTAGGTCAGGGCTCAAAGGAAAATGGGCTCAGGAAAACTTAGTTTGCCCAGCCTTGTCAGCTGAGATTGGTTTTGCTTTCCAGCCAGAAGTAGAAACTTG ATGCTTTTTCCTTTTTGCAGTGTTAAAAACCATATCTCCAGTTTTATTATGTTCCATCACAGTTTTGGGGCAAAGCCAGAAAGCTGACTTGTTTGCTTCA AAACACAAACCAAGGCACTGTCTTCTGTGCTTTTCAGGGAGGTGGGAAGGTAAAGGAGACTGCCCACTATAGGGGCTCAGTTCCCCCAAAGGCCTTGCAC ACCTTCTGATAAACCCCCTGGCTGAGGCAATCCTACTGGCTGTGCAGACAAAGATGTGACAGCAGCACTAGATGCTGACCCAACAAGCAGTGCTGGGCCT GGGCGGTGGGGCATGTTCAGCACTGCGGGCAGCAGTCAGCACATGCAAGACATTCAGTGAGCAGAGGGCTGCCCTCGGCCTCCAACTGCAGCCGCCCTGG CGCGTGAAATGAAATTGGTGGGCCTTCCTAGGATTGTAAAATGGGACTTGTCATTTGTGTGAGGTTGAAATCTACTATACTTTGAATATGGCAACTGTTT TCAACCACTGAAGCAAAGAAGGTAAAGGCCAGATGGGGAATGAAAGGTAAGGTACTGTGGTCTATTTCCAAATTTGGGAGCAATGTGTGTAACTTCAACT GTTGGAGTAAATGAGTTATGGAAAAGCCACAATTTCTAAAATCCCAAGGTTCTACCAAAGGAGTGAGGTTCCTGGGGAAACAGGCATGAGGCAGCAGCTC AGGCTGATGTGTCCTGTGCGATATAAAAAGCTGTGGCCTGATCTCTAAAGTGGGGCTTTTGAAAATCACCAGTCCACAACTACCTGCCTGGCTAGTTTAA CCTAGACTGCTTCAGAAGCAAGGGAGGGGGATAATGGACTGGAGAAAAAGCTGACACAAAACTCAAGACAACCAGTTTCAGTACCTGGGCCCTCAAAGCA ATAGTGAGGACTGAGAAAGTAGCAAGGGGCACTGATGTCATCTATTTTCTGGGAAGTGTAATTTTTAATGGTTTCATTAAAAACTTACTAACCTTCTACT GGAAGAATACCTCAAATGAAAAATTTTTTTTTTTTGAGACAGTTTTCGCTCTTGTTGCCCAGGGTGGAGTGCAGTGGCGCGATCTCGGCTCACTGTAACC TCTGCCTCCTGGGTTCAAGTGATTCTCCTGCCTCAGCCTCCCGAGTAAGTGGGATTACAGGCACGCACCACCAGACCTGGCTAACTTTCTTTGTATTTTT AGTAGAGATGGGGTTTCACCATGTTGGCCAGGCTGGTCTCCAACTCCTGACCTCAGGTGATCCGCCCACCTCGGCCTCCCAAAGTGTTGGGATTACAGGC GTGAGCCACTGCGCCCGGCCTCAACTGAAAATTCTTGATGAGATTAGTCCCACTGCATAGGTCTCAGCACTGAGGTTTAGAGATGTGAGACTCAGAGTAG GTTTCAGTTCCCCGGAGTGAAGGGGGCATAGGGAGGGGCAGGGCTTCCCAGTGTTAACTGCCTCTGCCCAAATCATGACTCAGTCCTGGTACCACGTCAA GATCCCTTTGAAAGGAAACATACTTTGCATCAAGTAATCCTTTGAAACTGCCACATTGAAAAGTTAGTTTTTAACCCAAATATAAATCTGGGAGCTAAAC ACTAGTCACGAAGCCATCTTTACTTAATGAAGAACCACCTTGAGGGAGACCAGCCCTGCTTGCCACTTACATGGCTGCCCCTGAGACGGTCTGTTCTGTT CATGCCCCAGCAGCCTTTTGCCTTAGGTTTCAGGACCTTCTGAACCACCCTTGAGGGTGTGTGCCAGCAAATACCTCAAGTGGACTTCTACTTAGGACCA CTTGAGGGCACAACTTCAGCTTCTAGAAGGCCAAGTGTGGCTGCAATTCAGGATCTGCCCCAAACTCATCAGCCCTTCCCAAGTTTCCCAGGTGAGAACA GTATGAGCTGTGAATTTCTGTGTTGGTGTGACAGGAATGCCACAGTGGTGGTGCCAGTGATCACTTTCTGACCTTCCTTCCTTCTCAGATAAAATGTTGC ACAGTTATGGCCTTGGAGGCCTAAGTAGACAGCAATTAACCAGCCAGTGAGAAAACGAATCAGCATCTAGAACAGGAGGGGAGGGAACCGTAACAGATAA GGGAGCCCACGATTAGAAGCAGGCTCCCTGCACAGCCTCTGCCTCCAGCGCAAGTAACAGACGTTTCACATTTAAAGGCTGCCAAGGTATTGCTGGCGAC TCTCCTGTCTGTGAACATGCGCAACGGGCACAGGTACAGAGAACCAATGAGATGTAGGCAGCACCATCTGCCACAAAAGGTTCTCCATCGCAGCCCCAGG AGCAAAATCAAACACCTCTGATGGGAGCAAGGACCCTGAACAGAAGCCAACGGCTGGAGCCAACGGGCCATGCCCTTAAAACGAGGACTTGGACAAGGAG GGAACCACTGGAGTGACGTGATGCAAGCCCTCTTCGGTCACTCTTGGCTTCCACCAGGGGTCTCTGCCTCCTTGATGAGAAGAGAAGCTGGCTGGCTCCA TGATGACCTCGATGGCGTACTACAGGCCGATGGGCAGGCCGGGCCTCAGCAGCAGGCGCCAACAGAAGCACACCCAGGGCTGATTCAGGTCCATTTCAGC ATAATTTTTGTGGAACAAATTAAAGAAGCAGTAATACCAGAGCTGTCCCCACTTAGGGAATAAAATTTTCAAGGCTGCAAGAAACTCTCTGTCTACAAAC CAGTGGCCATTGCCTAAGGAGGCTCGGGGAGGCCAGGGGATCACTATCAGTGGGATAGGTGCTGGCGGAACCAGGGGGTGTCTCCTTCCCAGTTTCAAAC TTGTCCTCAGAAGGGGGACTCAACTGGGGACGGCCGTGAGCTGTGGTGACTGAGGTGCACACAGCCTCCCAGAGGCAGAACCTGAAGAGCGCCACGTGGT AGCTCATCCAGGAAATCCGTGCTATTTGAGTTCTCATCGGCTGGGCCAACCACCTGCACCAGCTGACTAAACAGAACCGTTGCTGTCCCAAGCCCACGAG ACCCAGTGAGAGG

SHMT1.u.f.24 (forward primer)
SHMT1.u.r.18 (reverse primer)
Single exon skipping

Frame 0

RGRGQAQAWRAECTFLSSSGPAPSSGFGVGAAG HVAGPRRCAGRWVSGSGTGGTGGGVGRRRR*GLV LLARLECCGKIWAHCSLNLRDSSDHPASATQSS *EYRRAPPGSGSFEPVQ*RCQSTGPTRMLTCGP HMTRCWHNPSKTVMLRFTTSLRRRVTGRGLDWS* LPRRISPAEQFWRP*ALA*ITNTLRGTRARDTM AGLSLLMNWRPSVRSEPCRPISWTHSAGGSTSS PTQAPLQTLLCTLPWWNPMGASWAWTFRMGAT*P MGS*QTRRKSLPRPSSLNLCPTR*TQILATSTM TSWRRTHASSTRS*SSQEPAATPETWNMPGYGR LQMRTGRISWRTWLTSAGWWRLAWCPPHLNTAMW *PPPLTRPCEAAELA*SSTGKE*KVWIPRLAKR FCTTWSLLSILLCSLACREVPTTTPLLGLLWH* SKL*LWNLKFINTRWWPTAGLCLRP*RSWATK*S QVVLTTI*SLWISVPKAQMVEGLRRC*KPVLLP ATRTPVQVTEALCGPVDCGWGPQH*RPVDFWKK TSTTLVSEPP*KSSRRDWQGISTRRPCRLSGRRL RASPLSSLCLACLTSKGAGPLWTHLAPQRKLPA GGPPPERWMSCSKGELLTLGPFEGVSFGLFSCF LHKSKFV*VSLLVILGQVIKGFKFEPGFLTAGHN SRKIKYYVATWS*SFRWWCRAKLLERL*RFTLP GLSSALLQQRGNAHVHSLYTLPPHYLVIQWHAK GELN*LLRLLL*IRSVC*SRVNKMHPVWWEGFAV SSSKYGVEMASSLHPGKSQSHPGVRINQAFLLH LR*PTPSRNQEAKCHLPKEILFSRS*SAKHR*P FLISTIKFLTTECLQEL*L*KIQPIFIFQKYLNS NKLIS*KVHFSYYVFGIDSG*LLCSNTYEMLSH TRQLHSCTSRCNDCTSRGGGDSHSQDGTGTCSN KIAF*KITHINSSHRIMLFTATLKVAFISICDSS YLVADNPGEAACGPTL*VAAPAIWGLACRRLLY CGWSSCCRICELTHQSLQFCYQSGLQFRGLTPP QLETSCHLQGSL*TASTLGLNKHSQEAGPLPTD* SAAECSSPPHTPIHPCLLLLSTALTKP**GKAR LGQGSKENGLRKT*FAQPCQLRLVLLSSQK*KL DAFSFLQC*KPYLQFYYVPSQFWGKARKLTCLLQ NTNQGTVFCAFQGGGKVKETAHYRGSVPPKALH TF**TPWLRQSYWLCRQRCDSSTRC*PNKQCWA WAVGHVQHCGQQSAHARHSVSRGLPSASNCSRPG A*NEIGGPS*DCKMGLVICVRLKSTIL*IWQLF STTEAKKVKARWGMKGKVLWSISKFGSNVCNFN CWSK*VMEKPQFLKSQGSTKGVRFLGKQA*GSSS G*CVLCDIKSCGLISKVGLLKITSPQLPAWLV* PRLLQKQGRGIMDWRKS*HKTQDNQFQYLGPQS NSED*ESSKGH*CHLFSGKCNF*WFH*KLTNLLL EEYLK*KIFFF*DSFRSCCPGWSAVARSRLTVT SASWVQVILLPQPPE*VGLQARTTRPG*LSLYF **RWGFTMLARLVSNS*PQVIRPPRPPKVLGLQA *ATAPGLN*KFLMRLVPLHRSQH*GLEM*DSE* VSVPRSEGGIGRGRASQC*LPLPKS*LSPGTTS RSL*KETYFASSNPLKLPH*KVSF*PKYKSGS*T LVTKPSLLNEEPP*GRPALLATYMAAPETVCSV HAPAAFCLRFQDLLNHP*GCVPANTSSGLLLRT T*GHNFSF*KAKCGCNSGSAPNSSALPKFPR*EQ YEL*ISVLV*QECHSGGASDHFLTFLPSQIKCC TVMALEA*VDSN*PASEKTNQHLEQEGREP*QI REPTIRSRLPAQPLPPAQVTDVSHLKAAKVLLAT LLSVNMRNGHRYREPMRCRQHHLPQKVLHRSPR SKIKHL*WEQGP*TEANGWSQRAMPLKRGLGQG GNHWSDVMQALFGHSWLPPGVSASLMRREAGWLH DDLDGVLQADGQAGPQQQAPTEAHPGLIQVHFS IIFVEQIKEAVIPELSPLRE*NFQGCKKLSVYK PVAIA*GGSGRPGDHYQWDRCWRNQGVSPSQFQT CPQKGDSTGDGRELW*LRCTQPPRGRT*RAPRG SSSRKSVLFEFSSAGPTTCTS*LNRTVAVPSPR DPVR

Frame 1

GGGAKPKPGAQSAPS*ARAVQRQVRGLGLERLV TWLARGGARGVGSAGLGLVAPAAA*DGGVARVL FSWPGWSAVGKSGLTAASTSGTQVIILPQPPRVA ENTGVRHQARAASNQCNDDASQRGPQGC*PVVL T*QDAGTTPQRQ*C*GLQHH*EGE*PAEGWIGA DCLGEFRQPSSFGGPRLLLK*QIL*GVPGPEILW RD*VY**TGDPLSEASPAGL*AGPTVLGGQRPA LLRLPCKLCCVHCPGGTPWAHHGPGPSGWGPPD PWVHDRQEENLCHVHLL*IYALQGEPRYWLHQL* PAGGERTPLPPEADHRRNQLLLPKPGICPATED CR*ERGVSHGGHGSHQRAGGGWRGALPI*TLPC GDHHHSQDPARLPSWHDLLQERSEKCGSQDWQRD SVQPGVSYQFCCVPWPAGRSPQPRHCWGCCGTE ASYDSGI*SLSTPGGGQLQGSV*GPDGAGLQNS HRWF*QPFDPCGSPFQRHRWWKG*EGARSLFYCL QQEHLSR*QKRSAAQWTAAGDPSTDVPWTFGKR LPRHWCQSHPERVQGETGRG*VPGGRAGSPGGG *ELRLSLPSAWPA*LLKERAHSGPTWRHRGSCLP EDPHLRDG*AAPKGNC*HSGPLRGFLLDFFHVF FTNQNLFKSHC**FWDRLLKDLNLNLAFSQLDI ILGK*NTMSPLGHNHLDGGVGQSC*KDCSVLLSL GFPPPCCNREEMPMSTACTHCPLTILLSSGMPK EN*ISF*GFCCKSEVYVSQE*TRCTQYGGRVLL SVAQSMV*KWPPPSILGSPSPILV*ESTRLSCST *DNQLPPVIRKPNVTFPKKFYFHVAEVQNIDDH F**AQSNF*PQNVYKNYSFKKYNQFLYFKNI*T QIN*FLKKYTSHTMCLALTLVNSCAATPTRCYHT RASCTPAPAAATTAPHVVAGTHIHKTGREHAVI K*LFKK*HI*IHHIGLCFSLQP*RWHLSQSVTP AI*WQTIPVRQPVVPPCRWQPLQSGV*PVGVCFI VGGAAAAESVS*LTSPYSSATKVVFNLGA*PLP SWRQAAISRARCKQPPLLG*ISTPKKLGPYQQI NRLQSAHPLPTPPSTPASSSFPLL*PNLNEVRPG *VRAQRKMGSGKLSLPSLVS*DWFCFPARSRNL MLFPFCSVKNHISSFIMFHHSFGAKPES*LVCF KTQTKALSSVLFREVGR*RRLPTIGAQFPQRPCT PSDKPPG*GNPTGCADKDVTAALDADPTSSAGP GRWGMFSTAGSSQHMQDIQ*AEGCPRPPTAAAL AREMKLVGLPRIVKWDLSFV*G*NLLYFEYGNCF QPLKQRR*RPDGE*KVRYCGLFPNLGAMCVTST VGVNELWKSHNF*NPKVLPKE*GSWGNRHEAAA QADVSCAI*KAVA*SLKWGF*KSPVHNYLPG*FN LDCFRSKGGG*WTGEKADTKLKTTSFSTWALKA IVRTEKVARGTDVIYFLGSVIFNGFIKNLLTFY WKNTSNEKFFFFETVFALVAQGGVQWRDLGSL*P LPPGFK*FSCLSLPSKWDYRHAPPDLANFLCIF SRDGVSPCWPGWSPTPDLR*SAHLGLPKCWDYR REPLRPASTENS**D*SHCIGLSTEV*RCETQSR FQFPGVKGA*GGAGLPSVNCLCPNHDSVLVPRQ DPFERKHTLHQVIL*NCHIEKLVFNPNINLGAK H*SRSHLYLMKNHLEGDQPCLPLTWLPLRRSVLF MPQQPFALGFRTF*TTLEGVCQQIPQVDFYLGP LEGTTSASRRPSVAAIQDLPQTHQPFPSFPGEN SMSCEFLCWCDRNATVVVPVITF*PSFLLR*NVA QLWPWRPK*TAINQPVRKRISI*NRRGGNRNR* GSPRLEAGSLHSLCLQRK*QTFHI*RLPRYCWR LSCL*TCATGTGTENQ*DVGSTICHKRFSIAAPG AKSNTSDGSKDPEQKPTAGANGPCP*NEDLDKE GTTGVT*CKPSSVTLGFHQGSLPP**EEKLAGS MMTSMAYYRPMGRPGLSSRRQQKHTQG*FRSISA *FLWNKLKKQ*YQSCPHLGNKIFKAARNSLSTN QWPLPKEARGGQGITISGIGAGGTRGCLLPSFK LVLRRGTQLGTAVSCGD*GAHSLPEAEPEERHVV AHPGNPCYLSSHRLGQPPAPAD*TEPLLSQAHE TQ*E

Frame 2

GAGPSPSLARRVHLPELERSSAKFGVWGWSGWS RGWPAAVRGALGQRVWDWWHRRRRRTEASLGSC SLGQAGVLWENLGSLQPQPPGLK*SSCLSHPE* LRIQACATRLGQLRTSAMTMPVNGAHKDADLWSS HDKMLAQPLKDSDVEVYNIIKKESNRQRVGLEL IASENFASRAVLEALGSCLNNKYSEGYPGQRYY GGTEFIDELETLCQKRALQAYKLDPQCWGVNVQP YSGSPANFAVYTALVEPHGRIMGLDLPDGGHLT HGFMTDKKKISATSIFFESMPYKVNPDTGYINY DQLEENARLFHPKLIIAGTSCYSRNLEYARLRKI ADENGAYLMADMAHISGLVAAGVVPSPFEHCHV VTTTTHKTLRGCRAGMIFYRKGVKSVDPKTGKE ILYNLESLINSAVFPGLQGGPHNHAIAGVAVALK QAMTLEFKVYQHQVVANCRALSEALTELGYKIV TGGSDNHLILVDLRSKGTDGGRAEKVLEACSIA CNKNTCPGDRSALRPSGLRLGTPALTSRGLLEKD FHDTGVRATLKEFKERLAGDKYQAAVQALREEV ESFASLFPLPGLPDF*RSGPTLDPPGATEEAAC RRTPT*EMDELLQRGTVDTRAL*GGFFWTFFMFS SQIKICLSLIVSNSGTGY*RI*I*TWLSHSWT* F*ENKILCRHLVIII*MVV*GKAVRKIVAFYSP WAFLRLAATERKCPCPQLVHTAPSLSCYPVACQR RTELASEASAVNQKCMLVKSKQDAPSMVGGFCC Q*LKVWCRNGLLPPSWEVPVPSWCENQPGFPAP PEITNSLP*SGSQMSPSQRNFIFT*LKCKT*MTI FNKHNQIFNHRMSTRIIALKNTTNFYISKIFEL K*INFLKSTLLILCVWH*LWLTLVQQHLRDAIT HAPAALLHQPLQRLHLTWWRGLTFTRRDGNMQ** NSFLKNNTYKFIT*DYAFHCNLEGGIYLNL*LQ LFSGRQSR*GSLWSHLVGGSPCNLGSSLSAFAL LWVEQLLQNL*VDSPVPTVLLPKWSSI*GPDPSP AGDKLPSPGLAVNSLHSWAE*ALPRSWAPTNRL IGCRVLIPSPHPHPPLPPPPFHCFNQTLMR*GQ ARSGLKGKWAQENLVCPALSAEIGFAFQPEVET* CFFLFAVLKTISPVLLCSITVLGQSQKADLFAS KHKPRHCLLCFSGRWEGKGDCPL*GLSSPKGLA HLLINPLAEAILLAVQTKM*QQH*MLTQQAVLGL GGGACSALRAAVSTCKTFSEQRAALGLQLQPPW RVK*NWWAFLGL*NGTCHLCEVEIYYTLNMATV FNH*SKEGKGQMGNER*GTVVYFQIWEQCV*LQL LE*MSYGKATISKIPRFYQRSEVPGETGMRQQL RLMCPVRYKKLWPDL*SGAFENHQSTTTCLASL T*TASEAREGDNGLEKKLTQNSRQPVSVPGPSKQ **GLRK*QGALMSSIFWEV*FLMVSLKTY*PST GRIPQMKNFFFLRQFSLLLPRVECSGAISAHCN LCLLGSSDSPASASRVSGITGTHHQTWLTFFVFL VEMGFHHVGQAGLQLLTSGDPPTSASQSVGITG VSHCARPQLKILDEISPTA*VSALRFRDVRLRV GFSSPE*RGHREGQGFPVLTASAQIMTQSWYHVK IPLKGNILCIK*SFETATLKS*FLTQI*IWELN TSHEAIFT**RTTLRETSPACHLHGCP*DGLFC SCPSSLLP*VSGPSEPPLRVCASKYLKWTST*DH LRAQLQLLEGQVWLQFRICPKLISPSQVSQVRT V*AVNFCVGVTGMPQWWCQ*SLSDLPSFSDKML HSYGLGGLSRQQLTSQ*ENESASRTGGEGTVTDK GAHD*KQAPCTASASSASNRRFTFKGCQGIAGD SPVCEHAQRAQVQRTNEM*AAPSATKGSPSQPQ EQNQTPLMGARTLNRSQRLEPTGHALKTRTWTRR EPLE*RDASPLRSLLASTRGLCLLDEKRSWLAP **PRWRTTGRWAGRASAAGANRSTPRADSGPFQ HNFCGTN*RSSNTRAVPT*GIKFSRLQETLCLQT SGHCLRRLGEARGSLSVG*VLAEPGGVSFPVSN LSSEGGLNWGRP*AVVTEVHTASQRQNLKSATW *LIQEIRAI*VLIGWANHLHQLTKQNRCCPKPTR PSER

Sequence SHMT1.aAug05

CGGGGGCGGGGCCAAGCCCAAGCCTGGCGCGCAGAGTGCACCTTCCTGAGCTCGAGCGGTCCAGCGCCAAGTTCGGGGTTTGGGGTTGGAGCGGCTGGTC ACGTGGCTGGCCCGCGGCGGTGCGCGGGGCGTTGGGTCAGCGGGTCTGGGACTGGTGGCACCGGCGGCGGCGTAGGACGGAGGCGTCGCTAGGCAGCTTC GAACCAGTGCAATGACGATGCCAGTCAACGGGGCCCACAAGGATGCTGACCTGTGGTCCTCACATGACAAGATGCTGGCACAACCCCTCAAAGACAGTGA TGTTGAGGTTTACAACATCATTAAGAAGGAGAGTAACCGGCAGAGGGTTGGATTGGAGCTGATTGCCTCGGAGAATTTCGCCAGCCGAGCAGTTTTGGAG GCCCTAGGCTCTTGCTTAAATAACAAATACTCTGAGGGGTACCCGGGCCAGAGATACTATGGCGGGACTGAGTTTATTGATGAACTGGAGACCCTCTGTC AGAAGCGAGCCCTGCAGGCCTATAAGCTGGACCCACAGTGCTGGGGGGTCAACGTCCAGCCCTACTCAGGCTCCCCTGCAAACTTTGCTGTGTACACTGC CCTGGTGGAACCCCATGGGCGCATCATGGGCCTGGACCTTCCGGATGGGGGCCACCTGACCCATGGGTTCATGACAGACAAGAAGAAAATCTCTGCCACG TCCATCTTCTTTGAATCTATGCCCTACAAGGTGAACCCAGATACTGGCTACATCAACTATGACCAGCTGGAGGAGAACGCACGCCTCTTCCACCCGAAGC TGATCATCGCAGGAACCAGCTGCTACTCCCGAAACCTGGAATATGCCCGGCTACGGAAGATTGCAGATGAGAACGGGGCGTATCTCATGGCGGACATGGC TCACATCAGCGGGCTGGTGGCGGCTGGCGTGGTGCCCTCCCCATTTGAACACTGCCATGTGGTGACCACCACCACTCACAAGACCCTGCGAGGCTGCCGA GCTGGCATGATCTTCTACAGGAAAGGAGTGAAAAGTGTGGATCCCAAGACTGGCAAAGAGATTCTGTACAACCTGGAGTCTCTTATCAATTCTGCTGTGT TCCCTGGCCTGCAGGGAGGTCCCCACAACCACGCCATTGCTGGGGTTGCTGTGGCACTGAAGCAAGCTATGACTCTGGAATTTAAAGTTTATCAACACCA GGTGGTGGCCAACTGCAGGGCTCTGTCTGAGGCCCTGACGGAGCTGGGCTACAAAATAGTCACAGGTGGTTCTGACAACCATTTGATCCTTGTGGATCTC CGTTCCAAAGGCACAGATGGTGGAAGGGCTGAGAAGGTGCTAGAAGCCTGTTCTATTGCCTGCAACAAGAACACCTGTCCAGGTGACAGAAGCGCTCTGC GGCCCAGTGGACTGCGGCTGGGGACCCCAGCACTGACGTCCCGTGGACTTTTGGAAAAAGACTTCCAAAAAGTAGCCCACTTTATTCACAGAGGGATAGA GCTGACCCTGCAGATCCAGAGCGACACTGGTGTCAGAGCCACCCTGAAAGAGTTCAAGGAGAGACTGGCAGGGGATAAGTACCAGGCGGCCGTGCAGGCT CTCCGGGAGGAGGTTGAGAGCTTCGCCTCTCTCTTCCCTCTGCCTGGCCTGCCTGACTTCTAAAGGAGCGGGCCCACTCTGGACCCACCTGGCGCCACAG AGGAAGCTGCCTGCCGGAGGACCCCCACCTGAGAGATGGATGAGCTGCTCCAAAGGGGAACTGTTGACACTCGGGCCCTTTGAGGGGGTTTCTTTTGGAC TTTTTTCATGTTTTCTTCACAAATCAAAATTTGTTTAAGTCTCATTGTTAGTAATTCTGGGACAGGTTATTAAAGGATTTAAATTTGAACCTGGCTTTCT CACAGCTGGACATAATTCTAGGAAAATAAAATACTATGTCGCCACTTGGTCATAATCATTTAGATGGTGGTGTAGGGCAAAGCTGTTAGAAAGATTGTAG CGTTTTACTCTCCCTGGGCTTTCCTCCGCCTTGCTGCAACAGAGAGGAAATGCCCATGTCCACAGCTTGTACACACTGCCCCCTCACTATCTTGTTATCC AGTGGCATGCCAAAGGAGAACTGAATTAGCTTCTGAGGCTTCTGCTGTAAATCAGAAGTGTATGTTAGTCAAGAGTAAACAAGATGCACCCAGTATGGTG GGAGGGTTTTGCTGTCAGTAGCTCAAAGTATGGTGTAGAAATGGCCTCCTCCCTCCATCCTGGGAAGTCCCAGTCCCATCCTGGTGTGAGAATCAACCAG GCTTTCCTGCTCCACCTGAGATAACCAACTCCCTCCCGTAATCAGGAAGCCAAATGTCACCTTCCCAAAGAAATTTTATTTTCACGTAGCTGAAGTGCAA AACATAGATGACCATTTTTAATAAGCACAATCAAATTTTTAACCACAGAATGTCTACAAGAATTATAGCTTTAAAAAATACAACCAATTTTTATATTTCA AAAATATTTGAACTCAAATAAATTAATTTCTTAAAAAGTACACTTCTCATACTATGTGTTTGGCATTGACTCTGGTTAACTCTTGTGCAGCAACACCTAC GAGATGCTATCACACACGCGCCAGCTGCACTCCTGCACCAGCCGCTGCAACGACTGCACCTCACGTGGTGGCGGGGACTCACATTCACAAGACGGGACGG GAACATGCAGTAATAAAATAGCTTTTTAAAAAATAACACATATAAATTCATCACATAGGATTATGCTTTTCACTGCAACCTTGAAGGTGGCATTTATCTC AATCTGTGACTCCAGCTATTTAGTGGCAGACAATCCCGGTGAGGCAGCCTGTGGTCCCACCTTGTAGGTGGCAGCCCCTGCAATCTGGGGTCTAGCCTGT CGGCGTTTGCTTTATTGTGGGTGGAGCAGCTGCTGCAGAATCTGTGAGTTGACTCACCAGTCCCTACAGTTCTGCTACCAAAGTGGTCTTCAATTTAGGG GCCTGACCCCTCCCCAGCTGGAGACAAGCTGCCATCTCCAGGGCTCGCTGTAAACAGCCTCCACTCTTGGGCTGAATAAGCACTCCCAAGAAGCTGGGCC CCTACCAACAGATTAATCGGCTGCAGAGTGCTCATCCCCTCCCCACACCCCCATCCACCCCTGCCTCCTCCTCCTTTCCACTGCTTTAACCAAACCTTAA TGAGGTAAGGCCAGGCTAGGTCAGGGCTCAAAGGAAAATGGGCTCAGGAAAACTTAGTTTGCCCAGCCTTGTCAGCTGAGATTGGTTTTGCTTTCCAGCC AGAAGTAGAAACTTGATGCTTTTTCCTTTTTGCAGTGTTAAAAACCATATCTCCAGTTTTATTATGTTCCATCACAGTTTTGGGGCAAAGCCAGAAAGCT GACTTGTTTGCTTCAAAACACAAACCAAGGCACTGTCTTCTGTGCTTTTCAGGGAGGTGGGAAGGTAAAGGAGACTGCCCACTATAGGGGCTCAGTTCCC CCAAAGGCCTTGCACACCTTCTGATAAACCCCCTGGCTGAGGCAATCCTACTGGCTGTGCAGACAAAGATGTGACAGCAGCACTAGATGCTGACCCAACA AGCAGTGCTGGGCCTGGGCGGTGGGGCATGTTCAGCACTGCGGGCAGCAGTCAGCACATGCAAGACATTCAGTGAGCAGAGGGCTGCCCTCGGCCTCCAA CTGCAGCCGCCCTGGCGCGTGAAATGAAATTGGTGGGCCTTCCTAGGATTGTAAAATGGGACTTGTCATTTGTGTGAGGTTGAAATCTACTATACTTTGA ATATGGCAACTGTTTTCAACCACTGAAGCAAAGAAGGTAAAGGCCAGATGGGGAATGAAAGGTAAGGTACTGTGGTCTATTTCCAAATTTGGGAGCAATG TGTGTAACTTCAACTGTTGGAGTAAATGAGTTATGGAAAAGCCACAATTTCTAAAATCCCAAGGTTCTACCAAAGGAGTGAGGTTCCTGGGGAAACAGGC ATGAGGCAGCAGCTCAGGCTGATGTGTCCTGTGCGATATAAAAAGCTGTGGCCTGATCTCTAAAGTGGGGCTTTTGAAAATCACCAGTCCACAACTACCT GCCTGGCTAGTTTAACCTAGACTGCTTCAGAAGCAAGGGAGGGGGATAATGGACTGGAGAAAAAGCTGACACAAAACTCAAGACAACCAGTTTCAGTACC TGGGCCCTCAAAGCAATAGTGAGGACTGAGAAAGTAGCAAGGGGCACTGATGTCATCTATTTTCTGGGAAGTGTAATTTTTAATGGTTTCATTAAAAACT TACTAACCTTCTACTGGAAGAATACCTCAAATGAAAAATTTTTTTTTTTTGAGACAGTTTTCGCTCTTGTTGCCCAGGGTGGAGTGCAGTGGCGCGATCT CGGCTCACTGTAACCTCTGCCTCCTGGGTTCAAGTGATTCTCCTGCCTCAGCCTCCCGAGTAAGTGGGATTACAGGCACGCACCACCAGACCTGGCTAAC TTTCTTTGTATTTTTAGTAGAGATGGGGTTTCACCATGTTGGCCAGGCTGGTCTCCAACTCCTGACCTCAGGTGATCCGCCCACCTCGGCCTCCCAAAGT GTTGGGATTACAGGCGTGAGCCACTGCGCCCGGCCTCAACTGAAAATTCTTGATGAGATTAGTCCCACTGCATAGGTCTCAGCACTGAGGTTTAGAGATG TGAGACTCAGAGTAGGTTTCAGTTCCCCGGAGTGAAGGGGGCATAGGGAGGGGCAGGGCTTCCCAGTGTTAACTGCCTCTGCCCAAATCATGACTCAGTC CTGGTACCACGTCAAGATCCCTTTGAAAGGAAACATACTTTGCATCAAGTAATCCTTTGAAACTGCCACATTGAAAAGTTAGTTTTTAACCCAAATATAA ATCTGGGAGCTAAACACTAGTCACGAAGCCATCTTTACTTAATGAAGAACCACCTTGAGGGAGACCAGCCCTGCTTGCCACTTACATGGCTGCCCCTGAG ACGGTCTGTTCTGTTCATGCCCCAGCAGCCTTTTGCCTTAGGTTTCAGGACCTTCTGAACCACCCTTGAGGGTGTGTGCCAGCAAATACCTCAAGTGGAC TTCTACTTAGGACCACTTGAGGGCACAACTTCAGCTTCTAGAAGGCCAAGTGTGGCTGCAATTCAGGATCTGCCCCAAACTCATCAGCCCTTCCCAAGTT TCCCAGGTGAGAACAGTATGAGCTGTGAATTTCTGTGTTGGTGTGACAGGAATGCCACAGTGGTGGTGCCAGTGATCACTTTCTGACCTTCCTTCCTTCT CAGATAAAATGTTGCACAGTTATGGCCTTGGAGGCCTAAGTAGACAGCAATTAACCAGCCAGTGAGAAAACGAATCAGCATCTAGAACAGGAGGGGAGGG AACCGTAACAGATAAGGGAGCCCACGATTAGAAGCAGGCTCCCTGCACAGCCTCTGCCTCCAGCGCAAGTAACAGACGTTTCACATTTAAAGGCTGCCAA GGTATTGCTGGCGACTCTCCTGTCTGTGAACATGCGCAACGGGCACAGGTACAGAGAACCAATGAGATGTAGGCAGCACCATCTGCCACAAAAGGTTCTC CATCGCAGCCCCAGGAGCAAAATCAAACACCTCTGATGGGAGCAAGGACCCTGAACAGAAGCCAACGGCTGGAGCCAACGGGCCATGCCCTTAAAACGAG GACTTGGACAAGGAGGGAACCACTGGAGTGACGTGATGCAAGCCCTCTTCGGTCACTCTTGGCTTCCACCAGGGGTCTCTGCCTCCTTGATGAGAAGAGA AGCTGGCTGGCTCCATGATGACCTCGATGGCGTACTACAGGCCGATGGGCAGGCCGGGCCTCAGCAGCAGGCGCCAACAGAAGCACACCCAGGGCTGATT CAGGTCCATTTCAGCATAATTTTTGTGGAACAAATTAAAGAAGCAGTAATACCAGAGCTGTCCCCACTTAGGGAATAAAATTTTCAAGGCTGCAAGAAAC TCTCTGTCTACAAACCAGTGGCCATTGCCTAAGGAGGCTCGGGGAGGCCAGGGGATCACTATCAGTGGGATAGGTGCTGGCGGAACCAGGGGGTGTCTCC TTCCCAGTTTCAAACTTGTCCTCAGAAGGGGGACTCAACTGGGGACGGCCGTGAGCTGTGGTGACTGAGGTGCACACAGCCTCCCAGAGGCAGAACCTGA AGAGCGCCACGTGGTAGCTCATCCAGGAAATCCGTGCTATTTGAGTTCTCATCGGCTGGGCCAACCACCTGCACCAGCTGACTAAACAGAACCGTTGCTG TCCCAAGCCCACGAGACCCAGTGAGAGG

SHMT1.u.f.24 (forward primer)
SHMT1.u.r.18 (reverse primer)
Single exon skipping

Frame 0

RGRGQAQAWRAECTFLSSSGPAPSSGFGVGAAG HVAGPRRCAGRWVSGSGTGGTGGGVGRRRR*AAS NQCNDDASQRGPQGC*PVVLT*QDAGTTPQRQ* C*GLQHH*EGE*PAEGWIGADCLGEFRQPSSFG GPRLLLK*QIL*GVPGPEILWRD*VY**TGDPLS EASPAGL*AGPTVLGGQRPALLRLPCKLCCVHC PGGTPWAHHGPGPSGWGPPDPWVHDRQEENLCH VHLL*IYALQGEPRYWLHQL*PAGGERTPLPPEA DHRRNQLLLPKPGICPATEDCR*ERGVSHGGHG SHQRAGGGWRGALPI*TLPCGDHHHSQDPARLP SWHDLLQERSEKCGSQDWQRDSVQPGVSYQFCCV PWPAGRSPQPRHCWGCCGTEASYDSGI*SLSTP GGGQLQGSV*GPDGAGLQNSHRWF*QPFDPCGS PFQRHRWWKG*EGARSLFYCLQQEHLSR*QKRSA AQWTAAGDPSTDVPWTFGKRLPKSSPLYSQRDR ADPADPERHWCQSHPERVQGETGRG*VPGGRAG SPGGG*ELRLSLPSAWPA*LLKERAHSGPTWRHR GSCLPEDPHLRDG*AAPKGNC*HSGPLRGFLLD FFHVFFTNQNLFKSHC**FWDRLLKDLNLNLAF SQLDIILGK*NTMSPLGHNHLDGGVGQSC*KDCS VLLSLGFPPPCCNREEMPMSTACTHCPLTILLS SGMPKEN*ISF*GFCCKSEVYVSQE*TRCTQYG GRVLLSVAQSMV*KWPPPSILGSPSPILV*ESTR LSCST*DNQLPPVIRKPNVTFPKKFYFHVAEVQ NIDDHF**AQSNF*PQNVYKNYSFKKYNQFLYF KNI*TQIN*FLKKYTSHTMCLALTLVNSCAATPT RCYHTRASCTPAPAAATTAPHVVAGTHIHKTGR EHAVIK*LFKK*HI*IHHIGLCFSLQP*RWHLS QSVTPAI*WQTIPVRQPVVPPCRWQPLQSGV*PV GVCFIVGGAAAAESVS*LTSPYSSATKVVFNLG A*PLPSWRQAAISRARCKQPPLLG*ISTPKKLG PYQQINRLQSAHPLPTPPSTPASSSFPLL*PNLN EVRPG*VRAQRKMGSGKLSLPSLVS*DWFCFPA RSRNLMLFPFCSVKNHISSFIMFHHSFGAKPES *LVCFKTQTKALSSVLFREVGR*RRLPTIGAQFP QRPCTPSDKPPG*GNPTGCADKDVTAALDADPT SSAGPGRWGMFSTAGSSQHMQDIQ*AEGCPRPP TAAALAREMKLVGLPRIVKWDLSFV*G*NLLYFE YGNCFQPLKQRR*RPDGE*KVRYCGLFPNLGAM CVTSTVGVNELWKSHNF*NPKVLPKE*GSWGNR HEAAAQADVSCAI*KAVA*SLKWGF*KSPVHNYL PG*FNLDCFRSKGGG*WTGEKADTKLKTTSFST WALKAIVRTEKVARGTDVIYFLGSVIFNGFIKN LLTFYWKNTSNEKFFFFETVFALVAQGGVQWRDL GSL*PLPPGFK*FSCLSLPSKWDYRHAPPDLAN FLCIFSRDGVSPCWPGWSPTPDLR*SAHLGLPK CWDYRREPLRPASTENS**D*SHCIGLSTEV*RC ETQSRFQFPGVKGA*GGAGLPSVNCLCPNHDSV LVPRQDPFERKHTLHQVIL*NCHIEKLVFNPNI NLGAKH*SRSHLYLMKNHLEGDQPCLPLTWLPLR RSVLFMPQQPFALGFRTF*TTLEGVCQQIPQVD FYLGPLEGTTSASRRPSVAAIQDLPQTHQPFPS FPGENSMSCEFLCWCDRNATVVVPVITF*PSFLL R*NVAQLWPWRPK*TAINQPVRKRISI*NRRGG NRNR*GSPRLEAGSLHSLCLQRK*QTFHI*RLP RYCWRLSCL*TCATGTGTENQ*DVGSTICHKRFS IAAPGAKSNTSDGSKDPEQKPTAGANGPCP*NE DLDKEGTTGVT*CKPSSVTLGFHQGSLPP**EE KLAGSMMTSMAYYRPMGRPGLSSRRQQKHTQG*F RSISA*FLWNKLKKQ*YQSCPHLGNKIFKAARN SLSTNQWPLPKEARGGQGITISGIGAGGTRGCL LPSFKLVLRRGTQLGTAVSCGD*GAHSLPEAEPE ERHVVAHPGNPCYLSSHRLGQPPAPAD*TEPLL SQAHETQ*E

Frame 1

GGGAKPKPGAQSAPS*ARAVQRQVRGLGLERLV TWLARGGARGVGSAGLGLVAPAAA*DGGVARQL RTSAMTMPVNGAHKDADLWSSHDKMLAQPLKDSD VEVYNIIKKESNRQRVGLELIASENFASRAVLE ALGSCLNNKYSEGYPGQRYYGGTEFIDELETLC QKRALQAYKLDPQCWGVNVQPYSGSPANFAVYTA LVEPHGRIMGLDLPDGGHLTHGFMTDKKKISAT SIFFESMPYKVNPDTGYINYDQLEENARLFHPK LIIAGTSCYSRNLEYARLRKIADENGAYLMADMA HISGLVAAGVVPSPFEHCHVVTTTTHKTLRGCR AGMIFYRKGVKSVDPKTGKEILYNLESLINSAV FPGLQGGPHNHAIAGVAVALKQAMTLEFKVYQHQ VVANCRALSEALTELGYKIVTGGSDNHLILVDL RSKGTDGGRAEKVLEACSIACNKNTCPGDRSAL RPSGLRLGTPALTSRGLLEKDFQKVAHFIHRGIE LTLQIQSDTGVRATLKEFKERLAGDKYQAAVQA LREEVESFASLFPLPGLPDF*RSGPTLDPPGAT EEAACRRTPT*EMDELLQRGTVDTRAL*GGFFWT FFMFSSQIKICLSLIVSNSGTGY*RI*I*TWLS HSWT*F*ENKILCRHLVIII*MVV*GKAVRKIV AFYSPWAFLRLAATERKCPCPQLVHTAPSLSCYP VACQRRTELASEASAVNQKCMLVKSKQDAPSMV GGFCCQ*LKVWCRNGLLPPSWEVPVPSWCENQP GFPAPPEITNSLP*SGSQMSPSQRNFIFT*LKCK T*MTIFNKHNQIFNHRMSTRIIALKNTTNFYIS KIFELK*INFLKSTLLILCVWH*LWLTLVQQHL RDAITHAPAALLHQPLQRLHLTWWRGLTFTRRDG NMQ**NSFLKNNTYKFIT*DYAFHCNLEGGIYL NL*LQLFSGRQSR*GSLWSHLVGGSPCNLGSSL SAFALLWVEQLLQNL*VDSPVPTVLLPKWSSI*G PDPSPAGDKLPSPGLAVNSLHSWAE*ALPRSWA PTNRLIGCRVLIPSPHPHPPLPPPPFHCFNQTL MR*GQARSGLKGKWAQENLVCPALSAEIGFAFQP EVET*CFFLFAVLKTISPVLLCSITVLGQSQKA DLFASKHKPRHCLLCFSGRWEGKGDCPL*GLSS PKGLAHLLINPLAEAILLAVQTKM*QQH*MLTQQ AVLGLGGGACSALRAAVSTCKTFSEQRAALGLQ LQPPWRVK*NWWAFLGL*NGTCHLCEVEIYYTL NMATVFNH*SKEGKGQMGNER*GTVVYFQIWEQC V*LQLLE*MSYGKATISKIPRFYQRSEVPGETG MRQQLRLMCPVRYKKLWPDL*SGAFENHQSTTT CLASLT*TASEAREGDNGLEKKLTQNSRQPVSVP GPSKQ**GLRK*QGALMSSIFWEV*FLMVSLKT Y*PSTGRIPQMKNFFFLRQFSLLLPRVECSGAI SAHCNLCLLGSSDSPASASRVSGITGTHHQTWLT FFVFLVEMGFHHVGQAGLQLLTSGDPPTSASQS VGITGVSHCARPQLKILDEISPTA*VSALRFRD VRLRVGFSSPE*RGHREGQGFPVLTASAQIMTQS WYHVKIPLKGNILCIK*SFETATLKS*FLTQI* IWELNTSHEAIFT**RTTLRETSPACHLHGCP* DGLFCSCPSSLLP*VSGPSEPPLRVCASKYLKWT ST*DHLRAQLQLLEGQVWLQFRICPKLISPSQV SQVRTV*AVNFCVGVTGMPQWWCQ*SLSDLPSF SDKMLHSYGLGGLSRQQLTSQ*ENESASRTGGEG TVTDKGAHD*KQAPCTASASSASNRRFTFKGCQ GIAGDSPVCEHAQRAQVQRTNEM*AAPSATKGS PSQPQEQNQTPLMGARTLNRSQRLEPTGHALKTR TWTRREPLE*RDASPLRSLLASTRGLCLLDEKR SWLAP**PRWRTTGRWAGRASAAGANRSTPRAD SGPFQHNFCGTN*RSSNTRAVPT*GIKFSRLQET LCLQTSGHCLRRLGEARGSLSVG*VLAEPGGVS FPVSNLSSEGGLNWGRP*AVVTEVHTASQRQNL KSATW*LIQEIRAI*VLIGWANHLHQLTKQNRCC PKPTRPSER

Frame 2

GAGPSPSLARRVHLPELERSSAKFGVWGWSGWS RGWPAAVRGALGQRVWDWWHRRRRRTEASLGSF EPVQ*RCQSTGPTRMLTCGPHMTRCWHNPSKTV MLRFTTSLRRRVTGRGLDWS*LPRRISPAEQFWR P*ALA*ITNTLRGTRARDTMAGLSLLMNWRPSV RSEPCRPISWTHSAGGSTSSPTQAPLQTLLCTL PWWNPMGASWAWTFRMGAT*PMGS*QTRRKSLPR PSSLNLCPTR*TQILATSTMTSWRRTHASSTRS *SSQEPAATPETWNMPGYGRLQMRTGRISWRTW LTSAGWWRLAWCPPHLNTAMW*PPPLTRPCEAAE LA*SSTGKE*KVWIPRLAKRFCTTWSLLSILLC SLACREVPTTTPLLGLLWH*SKL*LWNLKFINT RWWPTAGLCLRP*RSWATK*SQVVLTTI*SLWIS VPKAQMVEGLRRC*KPVLLPATRTPVQVTEALC GPVDCGWGPQH*RPVDFWKKTSKK*PTLFTEG* S*PCRSRATLVSEPP*KSSRRDWQGISTRRPCRL SGRRLRASPLSSLCLACLTSKGAGPLWTHLAPQ RKLPAGGPPPERWMSCSKGELLTLGPFEGVSFG LFSCFLHKSKFV*VSLLVILGQVIKGFKFEPGFL TAGHNSRKIKYYVATWS*SFRWWCRAKLLERL* RFTLPGLSSALLQQRGNAHVHSLYTLPPHYLVI QWHAKGELN*LLRLLL*IRSVC*SRVNKMHPVWW EGFAVSSSKYGVEMASSLHPGKSQSHPGVRINQ AFLLHLR*PTPSRNQEAKCHLPKEILFSRS*SA KHR*PFLISTIKFLTTECLQEL*L*KIQPIFIFQ KYLNSNKLIS*KVHFSYYVFGIDSG*LLCSNTY EMLSHTRQLHSCTSRCNDCTSRGGGDSHSQDGT GTCSNKIAF*KITHINSSHRIMLFTATLKVAFIS ICDSSYLVADNPGEAACGPTL*VAAPAIWGLAC RRLLYCGWSSCCRICELTHQSLQFCYQSGLQFR GLTPPQLETSCHLQGSL*TASTLGLNKHSQEAGP LPTD*SAAECSSPPHTPIHPCLLLLSTALTKP* *GKARLGQGSKENGLRKT*FAQPCQLRLVLLSS QK*KLDAFSFLQC*KPYLQFYYVPSQFWGKARKL TCLLQNTNQGTVFCAFQGGGKVKETAHYRGSVP PKALHTF**TPWLRQSYWLCRQRCDSSTRC*PN KQCWAWAVGHVQHCGQQSAHARHSVSRGLPSASN CSRPGA*NEIGGPS*DCKMGLVICVRLKSTIL* IWQLFSTTEAKKVKARWGMKGKVLWSISKFGSN VCNFNCWSK*VMEKPQFLKSQGSTKGVRFLGKQA *GSSSG*CVLCDIKSCGLISKVGLLKITSPQLP AWLV*PRLLQKQGRGIMDWRKS*HKTQDNQFQY LGPQSNSED*ESSKGH*CHLFSGKCNF*WFH*KL TNLLLEEYLK*KIFFF*DSFRSCCPGWSAVARS RLTVTSASWVQVILLPQPPE*VGLQARTTRPG* LSLYF**RWGFTMLARLVSNS*PQVIRPPRPPKV LGLQA*ATAPGLN*KFLMRLVPLHRSQH*GLEM *DSE*VSVPRSEGGIGRGRASQC*LPLPKS*LS PGTTSRSL*KETYFASSNPLKLPH*KVSF*PKYK SGS*TLVTKPSLLNEEPP*GRPALLATYMAAPE TVCSVHAPAAFCLRFQDLLNHP*GCVPANTSSG LLLRTT*GHNFSF*KAKCGCNSGSAPNSSALPKF PR*EQYEL*ISVLV*QECHSGGASDHFLTFLPS QIKCCTVMALEA*VDSN*PASEKTNQHLEQEGR EP*QIREPTIRSRLPAQPLPPAQVTDVSHLKAAK VLLATLLSVNMRNGHRYREPMRCRQHHLPQKVL HRSPRSKIKHL*WEQGP*TEANGWSQRAMPLKR GLGQGGNHWSDVMQALFGHSWLPPGVSASLMRRE AGWLHDDLDGVLQADGQAGPQQQAPTEAHPGLI QVHFSIIFVEQIKEAVIPELSPLRE*NFQGCKK LSVYKPVAIA*GGSGRPGDHYQWDRCWRNQGVSP SQFQTCPQKGDSTGDGRELW*LRCTQPPRGRT* RAPRGSSSRKSVLFEFSSAGPTTCTS*LNRTVA VPSPRDPVR