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Sequences

Sequence CHEK2.nAug05

GCCTTCTCTTCCGCCAGCCAGATCCGTCCTCCCCTCTCGCAGGCCAGACCTCGACGTGTCTCTCGCCCGGGCCCGTTTCCCGAAACCGTCGCTCGTACCA
GTCCATAAGGCTGAAGTAGTCTCGAGTGGGGTCAGGTGCCTGCAGCGCTCGGCACTGTGGGCAGAAGAACCTGTCCTCCCGCCCGGGGCCCCATGGGCCG
CCGCAGTTCCAACAGCGGGGATAATTGCTTCCCGCCTGCGACGCAGCATCGCAGCTTAGCGGTCTCCTTCTGGGAACCCCTGTCGGCCAAAACCCCCACA
CCCGGAGCAAAGCCCCGGCTCTCCCCCGCCACATCTGGCCGGCGGCCTATCTAGCCGTGGTCACTCGTGGGGAAAAGCAAAGAGAGCGTCTAACCAGACT
AATGTTGCTGATTGGCTGGGGAGTCGAGGGGGCGGGATCACCCGAGGGGAACCCGGGTTCTAAGTTCCGCTCTCCCTTCTAAACTACAACTCCCAGGAGG
CATTGAGGCGGCGCCTGACGGCCACATCTGCTGCTCCTCATTGGTCCGGCGGCAGGGGAGGGGGTTTTGATTGGCTGAGGGTGGAGTTTGTATCTGCAGG
TTTAGCGCCACTCTGCTGGCTGAGGCTGCGGAGAGTGTGCGGCTCCAGGTGGGCTCACGCGGTCGTGATGTCTCGGGAGTCGGATGTTGAGGCTCAGCAG
TCTCATGGCAGCAGTGCCTGTTCACAGCCCCATGGCAGCGTTACCCAGTCCCAAGGCTCCTCCTCACAGTCCCAGGGCATATCCAGCTCCTCTACCAGCA
CGATGCCAAACTCCAGCCAGTCCTCTCACTCCAGCTCTGGGACACTGAGCTCCTTAGAGACAGTGTCCACTCAGGAACTCTATTCTATTCCTGAGGACCA
AGAACCTGAGGACCAAGAACCTGAGGAGCCTACCCCTGCCCCCTGGGCTCGATTATGGGCCCTTCAGGATGGATTTGCCAATCTTGAGACAGAGTCTGGC
CATGTTACCCAATCTGATCTTGAACTCCTGCTGTCATCTGATCCTCCTGCCTCAGCCTCCCAAAGTGCTGGGATAAGAGAATGTGTGAATGACAACTACT
GGTTTGGGAGGGACAAAAGCTGTGAATATTGCTTTGATGAACCACTGCTGAAAAGAACAGATAAATACCGAACATACAGCAAGAAACACTTTCGGATTTT
CAGGGAAGTGGGTCCTAAAAACTCTTACATTGCATACATAGAAGATCACAGTGGCAATGGAACCTTTGTAAATACAGAGCTTGTAGGGAAAGGAAAACGC
CGTCCTTTGAATAACAATTCTGAAATTGCACTGTCACTAAGCAGAAATAAAGTTTTTGTCTTTTTTGATCTGACTGTAGATGATCAGTCAGTTTATCCTA
AGGCATTAAGAGATGAATACATCATGTCAAAAACTCTTGGAAG
  • CHEK2.u.f.17 (forward primer)
  • CHEK2.u.r.17 (reverse primer)
  • Alternative donor

Frame 0

AFSSASQIRPPLSQARPRRVSRPGPFPETVART
SP*G*SSLEWGQVPAALGTVGRRTCPPARGPMGR
RSSNSGDNCFPPATQHRSLAVSFWEPLSAKTPT
PGAKPRLSPATSGRRPI*PWSLVGKSKESV*PD
*CC*LAGESRGRDHPRGTRVLSSALPSKLQLPGG
IEAAPDGHICCSSLVRRQGRGF*LAEGGVCICR
FSATLLAEAAESVRLQVGSRGRDVSGVGC*GSA
VSWQQCLFTAPWQRYPVPRLLLTVPGHIQLLYQH
DAKLQPVLSLQLWDTELLRDSVHSGTLFYS*GP
RT*GPRT*GAYPCPLGSIMGPSGWICQS*DRVW
PCYPI*S*TPAVI*SSCLSLPKCWDKRMCE*QLL
VWEGQKL*ILL**TTAEKNR*IPNIQQETLSDF
QGSGS*KLLHCIHRRSQWQWNLCKYRACRERKT
PSFE*QF*NCTVTKQK*SFCLF*SDCR*SVSLS*
GIKR*IHHVKNSWK

Frame 1

PSLPPARSVLPSRRPDLDVSLARARFPKPSLVP
VHKAEVVSSGVRCLQRSALWAEEPVLPPGAPWA
AAVPTAGIIASRLRRSIAA*RSPSGNPCRPKPPH
PEQSPGSPPPHLAGGLSSRGHSWGKAKRASNQT
NVADWLGSRGGGITRGEPGF*VPLSLLNYNSQE
ALRRRLTATSAAPHWSGGRGGGFDWLRVEFVSAG
LAPLCWLRLRRVCGSRWAHAVVMSRESDVEAQQ
SHGSSACSQPHGSVTQSQGSSSQSQGISSSSTS
TMPNSSQSSHSSSGTLSSLETVSTQELYSIPEDQ
EPEDQEPEEPTPAPWARLWALQDGFANLETESG
HVTQSDLELLLSSDPPASASQSAGIRECVNDNY
WFGRDKSCEYCFDEPLLKRTDKYRTYSKKHFRIF
REVGPKNSYIAYIEDHSGNGTFVNTELVGKGKR
RPLNNNSEIALSLSRNKVFVFFDLTVDDQSVYP
KALRDEYIMSKTLG

Frame 2

LLFRQPDPSSPLAGQTSTCLSPGPVSRNRRSYQ
SIRLK*SRVGSGACSARHCGQKNLSSRPGPHGP
PQFQQRG*LLPACDAASQLSGLLLGTPVGQNPH
TRSKAPALPRHIWPAAYLAVVTRGEKQRERLTRL
MLLIGWGVEGAGSPEGNPGSKFRSPF*TTTPRR
H*GGA*RPHLLLLIGPAAGEGVLIG*GWSLYLQ
V*RHSAG*GCGECAAPGGLTRS*CLGSRMLRLSS
LMAAVPVHSPMAALPSPKAPPHSPRAYPAPLPA
RCQTPASPLTPALGH*AP*RQCPLRNSILFLRT
KNLRTKNLRSLPLPPGLDYGPFRMDLPILRQSLA
MLPNLILNSCCHLILLPQPPKVLG*ENV*MTTT
GLGGTKAVNIALMNHC*KEQINTEHTARNTFGF
SGKWVLKTLTLHT*KITVAMEPL*IQSL*GKENA
VL*ITILKLHCH*AEIKFLSFLI*L*MISQFIL
RH*EMNTSCQKLLE

Sequence CHEK2.aAug05

GCCTTCTCTTCCGCCAGCCAGATCCGTCCTCCCCTCTCGCAGGCCAGACCTCGACGTGTCTCTCGCCCGGGCCCGTTTCCCGAAACCGTCGCTCGTACCA
GTCCATAAGGCTGAAGTAGTCTCGAGTGGGGTCAGGTGCCTGCAGCGCTCGGCACTGTGGGCAGAAGAACCTGTCCTCCCGCCCGGGGCCCCATGGGCCG
CCGCAGTTCCAACAGCGGGGATAATTGCTTCCCGCCTGCGACGCAGCATCGCAGCTTAGCGGTCTCCTTCTGGGAACCCCTGTCGGCCAAAACCCCCACA
CCCGGAGCAAAGCCCCGGCTCTCCCCCGCCACATCTGGCCGGCGGCCTATCTAGCCGTGGTCACTCGTGGGGAAAAGCAAAGAGAGCGTCTAACCAGACT
AATGTTGCTGATTGGCTGGGGAGTCGAGGGGGCGGGATCACCCGAGGGGAACCCGGGTTCTAAGTTCCGCTCTCCCTTCTAAACTACAACTCCCAGGAGG
CATTGAGGCGGCGCCTGACGGCCACATCTGCTGCTCCTCATTGGTCCGGCGGCAGGGGAGGGGGTTTTGATTGGCTGAGGGTGGAGTTTGTATCTGCAGG
TTTAGCGCCACTCTGCTGGCTGAGGCTGCGGAGAGTGTGCGGCTCCAGGTGGGCTCACGCGGTCGTGATGTCTCGGGAGTCGGATGTTGAGGCTCAGCAG
TCTCATGGCAGCAGTGCCTGTTCACAGCCCCATGGCAGCGTTACCCAGTCCCAAGGCTCCTCCTCACAGTCCCAGGGCATATCCAGCTCCTCTACCAGCA
CGATGCCAAACTCCAGCCAGTCCTCTCACTCCAGCTCTGGGACACTGAGCTCCTTAGAGACAGTGTCCACTCAGGAACTCTATTCTATTCCTGAGGACCA
AGAACCTGAGGACCAAGAACCTGAGGAGCCTACCCCTGCCCCCTGGGCTCGATTATGGGCCCTTCAGGATGGATTTGCCAATCTTGAGACAGAGTCTGGC
CATGTTACCCAATCTGATCTTGAACTCCTGCTGTCATCTGATCCTCCTGCCTCAGCCTCCCAAAGTGCTGGGATAAGAGGTGTGAGGCACCATCCCCGGC
CAGTTTGCAGTCTAAAATGTGTGAATGACAACTACTGGTTTGGGAGGGACAAAAGCTGTGAATATTGCTTTGATGAACCACTGCTGAAAAGAACAGATAA
ATACCGAACATACAGCAAGAAACACTTTCGGATTTTCAGGGAAGTGGGTCCTAAAAACTCTTACATTGCATACATAGAAGATCACAGTGGCAATGGAACC
TTTGTAAATACAGAGCTTGTAGGGAAAGGAAAACGCCGTCCTTTGAATAACAATTCTGAAATTGCACTGTCACTAAGCAGAAATAAAGTTTTTGTCTTTT
TTGATCTGACTGTAGATGATCAGTCAGTTTATCCTAAGGCATTAAGAGATGAATACATCATGTCAAAAACTCTTGGAAGTGGTGCCTGTGGAGAGGTAAA
GCTGGCTTTCGAGAGGAAAACATGTAAGAAAGTAGCCATAAAGATCATCAGCAAAAGGAAGTTTGCTATTGGTTCAGCAAGAGAGGCAGACCCAGCTCTC
AATGTTGAAACAGAAATAGAAATTTTGAAAAAGCTAAATCATCCTTGCATCATCAAGATTAAAAACTTTTTTGATGCAGAAGATTATTATATTGTTTTGG
AATTGATGGAAGGGGGAGAGCTGTTTGACAAAGTGGTGGGGAATAAACGCCTGAAAGAAGCTACCTGCAAGCTCTATTTTTACCAGATGCTCTTGGCTGT
GCAGTACCTTCATGAAAACGGTATTATACACCGTGACTTAAAGCCAGAGAATGTTTTACTGTCATCTCAAGAAGAGGACTGTCTTATAAAGATTACTGAT
TTTGGGCACTCCAAGATTTTGGGAGAGACCTCTCTCATGAGAACCTTATGTGGAACCCCCACCTACTTGGCGCCTGAAGTTCTTGTTTCTGTTGGGACTG
CTGGGTATAACCGTGCTGTGGACTGCTGGAGTTTAGGAGTTATTCTTTTTATCTGCCTTAGTGGGTATCCACCTTTCTCTGAGCATAGGACTCAAGTGTC
ACTGAAGGATCAGATCACCAGTGGAAAATACAACTTCATTCCTGAAGTCTGGGCAGAAGTCTCAGAGAAAGCTCTGGACCTTGTCAAGAAGTTGTTGGTA
GTGGATCCAAAGGCACGTTTTACGACAGAAGAAGCCTTAAGACACCCGTGGCTTCAGGATGAAGACATGAAGAGAAAGTTTCAAGATCTTCTGTCTGAGG
AAAATGAATCCACAGCTCTACCCCAGGTTCTAGCCCAGCCTTCTACTAGTCGAAAGCGGCCCCGTGAAGGGGAAGCCGAGGGTGCCGAGACCACAAAGCG
CCCAGCTGTGTGTGCTGCTGTGTTGTGAACTCCGTGGTTTGAACACGAAAGAAATGTACCTTCTTTCACTCTGTCATCTTTCTTTTCTTTGAGTCTGTTT
TTTTATAGTTTGTATTTTAATTATGGGAATAATTGCTTTTTCACAGTCACTGATGTACAATTAAAAACCTGATGGAACCTGGGCTTTGTGCTTCTGCTTG
ATAATCGGTTCTTTAGTTGAATGGCTTTATTATTTATTTATTTGAGATGGAGTCTCACTCTGTTGCCCAGCCTGAATGAAGTGCAGTGGTGCAGGCTTGG
CTCACTGCAACCTCTGCTTCCCAGGTTCAAGCGATTCTTGTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGTATGCACCACCATGCCTGTTAATTTTT
ATATATTTTG
  • CHEK2.u.f.17 (forward primer)
  • CHEK2.u.r.17 (reverse primer)
  • Alternative donor

Frame 0

AFSSASQIRPPLSQARPRRVSRPGPFPETVART
SP*G*SSLEWGQVPAALGTVGRRTCPPARGPMGR
RSSNSGDNCFPPATQHRSLAVSFWEPLSAKTPT
PGAKPRLSPATSGRRPI*PWSLVGKSKESV*PD
*CC*LAGESRGRDHPRGTRVLSSALPSKLQLPGG
IEAAPDGHICCSSLVRRQGRGF*LAEGGVCICR
FSATLLAEAAESVRLQVGSRGRDVSGVGC*GSA
VSWQQCLFTAPWQRYPVPRLLLTVPGHIQLLYQH
DAKLQPVLSLQLWDTELLRDSVHSGTLFYS*GP
RT*GPRT*GAYPCPLGSIMGPSGWICQS*DRVW
PCYPI*S*TPAVI*SSCLSLPKCWDKRCEAPSPA
SLQSKMCE*QLLVWEGQKL*ILL**TTAEKNR*
IPNIQQETLSDFQGSGS*KLLHCIHRRSQWQWN
LCKYRACRERKTPSFE*QF*NCTVTKQK*SFCLF
*SDCR*SVSLS*GIKR*IHHVKNSWKWCLWRGK
AGFREENM*ESSHKDHQQKEVCYWFSKRGRPSS
QC*NRNRNFEKAKSSLHHQD*KLF*CRRLLYCFG
IDGRGRAV*QSGGE*TPERSYLQALFLPDALGC
AVPS*KRYYTP*LKARECFTVISRRGLSYKDY*
FWALQDFGRDLSHENLMWNPHLLGA*SSCFCWDC
WV*PCCGLLEFRSYSFYLP*WVSTFL*A*DSSV
TEGSDHQWKIQLHS*SLGRSLRESSGPCQEVVG
SGSKGTFYDRRSLKTPVASG*RHEEKVSRSSV*G
K*IHSSTPGSSPAFY*SKAAP*RGSRGCRDHKA
PSCVCCCVVNSVV*TRKKCTFFHSVIFLFFESV
FL*FVF*LWE*LLFHSH*CTIKNLMEPGLCASA*
*SVL*LNGFIIYLFEMESHSVAQPE*SAVVQAW
LTATSASQVQAILVPQPPE*LGLQVCTTMPVNF
YIF

Frame 1

PSLPPARSVLPSRRPDLDVSLARARFPKPSLVP
VHKAEVVSSGVRCLQRSALWAEEPVLPPGAPWA
AAVPTAGIIASRLRRSIAA*RSPSGNPCRPKPPH
PEQSPGSPPPHLAGGLSSRGHSWGKAKRASNQT
NVADWLGSRGGGITRGEPGF*VPLSLLNYNSQE
ALRRRLTATSAAPHWSGGRGGGFDWLRVEFVSAG
LAPLCWLRLRRVCGSRWAHAVVMSRESDVEAQQ
SHGSSACSQPHGSVTQSQGSSSQSQGISSSSTS
TMPNSSQSSHSSSGTLSSLETVSTQELYSIPEDQ
EPEDQEPEEPTPAPWARLWALQDGFANLETESG
HVTQSDLELLLSSDPPASASQSAGIRGVRHHPR
PVCSLKCVNDNYWFGRDKSCEYCFDEPLLKRTDK
YRTYSKKHFRIFREVGPKNSYIAYIEDHSGNGT
FVNTELVGKGKRRPLNNNSEIALSLSRNKVFVF
FDLTVDDQSVYPKALRDEYIMSKTLGSGACGEVK
LAFERKTCKKVAIKIISKRKFAIGSAREADPAL
NVETEIEILKKLNHPCIIKIKNFFDAEDYYIVL
ELMEGGELFDKVVGNKRLKEATCKLYFYQMLLAV
QYLHENGIIHRDLKPENVLLSSQEEDCLIKITD
FGHSKILGETSLMRTLCGTPTYLAPEVLVSVGT
AGYNRAVDCWSLGVILFICLSGYPPFSEHRTQVS
LKDQITSGKYNFIPEVWAEVSEKALDLVKKLLV
VDPKARFTTEEALRHPWLQDEDMKRKFQDLLSE
ENESTALPQVLAQPSTSRKRPREGEAEGAETTKR
PAVCAAVL*TPWFEHERNVPSFTLSSFFSLSLF
FYSLYFNYGNNCFFTVTDVQLKT*WNLGFVLLL
DNRFFS*MALLFIYLRWSLTLLPSLNEVQWCRLG
SLQPLLPRFKRFLCLSLLSSWDYRYAPPCLLIF
IYF

Frame 2

LLFRQPDPSSPLAGQTSTCLSPGPVSRNRRSYQ
SIRLK*SRVGSGACSARHCGQKNLSSRPGPHGP
PQFQQRG*LLPACDAASQLSGLLLGTPVGQNPH
TRSKAPALPRHIWPAAYLAVVTRGEKQRERLTRL
MLLIGWGVEGAGSPEGNPGSKFRSPF*TTTPRR
H*GGA*RPHLLLLIGPAAGEGVLIG*GWSLYLQ
V*RHSAG*GCGECAAPGGLTRS*CLGSRMLRLSS
LMAAVPVHSPMAALPSPKAPPHSPRAYPAPLPA
RCQTPASPLTPALGH*AP*RQCPLRNSILFLRT
KNLRTKNLRSLPLPPGLDYGPFRMDLPILRQSLA
MLPNLILNSCCHLILLPQPPKVLG*EV*GTIPG
QFAV*NV*MTTTGLGGTKAVNIALMNHC*KEQI
NTEHTARNTFGFSGKWVLKTLTLHT*KITVAMEP
L*IQSL*GKENAVL*ITILKLHCH*AEIKFLSF
LI*L*MISQFILRH*EMNTSCQKLLEVVPVER*
SWLSRGKHVRK*P*RSSAKGSLLLVQQERQTQLS
MLKQK*KF*KS*IILASSRLKTFLMQKIIILFW
N*WKGESCLTKWWGINA*KKLPASSIFTRCSWL
CSTFMKTVLYTVT*SQRMFYCHLKKRTVL*RLLI
LGTPRFWERPLS*EPYVEPPPTWRLKFLFLLGL
LGITVLWTAGV*ELFFLSALVGIHLSLSIGLKC
H*RIRSPVENTTSFLKSGQKSQRKLWTLSRSCW*
WIQRHVLRQKKP*DTRGFRMKT*RESFKIFCLR
KMNPQLYPRF*PSLLLVESGPVKGKPRVPRPQS
AQLCVLLCCELRGLNTKEMYLLSLCHLSFL*VCF
FIVCILIMGIIAFSQSLMYN*KPDGTWALCFCL
IIGSLVEWLYYLFI*DGVSLCCPA*MKCSGAGL
AHCNLCFPGSSDSCASAS*VAGITGMHHHAC*FL
YIL

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