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Sequences

Sequence CHEK2.lAug05

CHEK2.u.f.13 (forward primer)
CHEK2.u.r.25 (reverse primer)
Single exon skipping

Frame 0

VVMSRESDVEAQQSHGSSACSQPHGSVTQSQGS SSQSQGISSSSTSTMPNSSQSSHSSSGTLSSLET VSTQELYSIPEDQEPEDQEPEEPTPAPWARLWA LQDGFANLECVNDNYWFGRDKSCEYCFDEPLLK RTDKYRTYSKKHFRIFREENLSCPYRIWFNGACG EVKLAFERKTCKKVAIKIISKRKFAIGSAREAD PALNVETEIEILKKLNHPCIIKIKNFFDAEDYY IVLELMEGGELFDKVVGNKRLKEATCKLYFYQML LAVQYLHENGIIHRDLKPENVLLSSQEEDCLIK

Frame 1

S*CLGSRMLRLSSLMAAVPVHSPMAALPSPKAP PHSPRAYPAPLPARCQTPASPLTPALGH*AP*R QCPLRNSILFLRTKNLRTKNLRSLPLPPGLDYGP FRMDLPILNV*MTTTGLGGTKAVNIALMNHC*K EQINTEHTARNTFGFSGRKICPVLTEYGLMVPV ER*SWLSRGKHVRK*P*RSSAKGSLLLVQQERQT QLSMLKQK*KF*KS*IILASSRLKTFLMQKIII LFWN*WKGESCLTKWWGINA*KKLPASSIFTRC SWLCSTFMKTVLYTVT*SQRMFYCHLKKRTVL*

Frame 2

RDVSGVGC*GSAVSWQQCLFTAPWQRYPVPRLL LTVPGHIQLLYQHDAKLQPVLSLQLWDTELLRD SVHSGTLFYS*GPRT*GPRT*GAYPCPLGSIMG PSGWICQS*MCE*QLLVWEGQKL*ILL**TTAEK NR*IPNIQQETLSDFQGGKFVLSLQNMV*WCLW RGKAGFREENM*ESSHKDHQQKEVCYWFSKRGR PSSQC*NRNRNFEKAKSSLHHQD*KLF*CRRLLY CFGIDGRGRAV*QSGGE*TPERSYLQALFLPDA LGCAVPS*KRYYTP*LKARECFTVISRRGLSYK

Sequence CHEK2.iAug05

GTCGTGATGTCTCGGGAGTCGGATGTTGAGGCTCAGCAGTCTCATGGCAGCAGTGCCTGTTCACAGCCCCATGGCAGCGTTACCCAGTCCCAAGGCTCCT CCTCACAGTCCCAGGGCATATCCAGCTCCTCTACCAGCACGATGCCAAACTCCAGCCAGTCCTCTCACTCCAGCTCTGGGACACTGAGCTCCTTAGAGAC AGTGTCCACTCAGGAACTCTATTCTATTCCTGAGGACCAAGAACCTGAGGACCAAGAACCTGAGGAGCCTACCCCTGCCCCCTGGGCTCGATTATGGGCC CTTCAGGATGGATTTGCCAATCTTGAATGTGTGAATGACAACTACTGGTTTGGGAGGGACAAAAGCTGTGAATATTGCTTTGATGAACCACTGCTGAAAA GAACAGATAAATACCGAACATACAGCAAGAAACACTTTCGGATTTTCAGGGAGGAAAATTTGTCCTGTCCTTACAGAATATGGTTTAATTTTTGTCTTTT TTGATCTGACTGTAGATGATCAGTCAGTTTATCCTAAGGCATTAAGAGATGAATACATCATGTCAAAAACTCTTGGAAGTGGTGCCTGTGGAGAGGTAAA GCTGGCTTTCGAGAGGAAAACATGTAAGAAAGTAGCCATAAAGATCATCAGCAAAAGGAAGTTTGCTATTGGTTCAGCAAGAGAGGCAGACCCAGCTCTC AATGTTGAAACAGAAATAGAAATTTTGAAAAAGCTAAATCATCCTTGCATCATCAAGATTAAAAACTTTTTTGATGCAGAAGATTATTATATTGTTTTGG AATTGATGGAAGGGGGAGAGCTGTTTGACAAAGTGGTGGGGAATAAACGCCTGAAAGAAGCTACCTGCAAGCTCTATTTTTACCAGATGCTCTTGGCTGT GCAGTACCTTCATGAAAACGGTATTATACACCGTGACTTAAAGCCAGAGAATGTTTTACTGTCATCTCAAGAAGAGGACTGTCTTATAAAGATTACTGAT TTTGGGCACTCCAAGATTTTGGGAGAGACCTCTCTCATGAGAACCTTATGTGGAACCCCCACCTACTTGGCGCCTGAAGTTCTTGTTTCTGTTGGGACTG CTGGGTATAACCGTGCTGTGGACTGCTGGAGTTTAGGAGTTATTCTTTTTATCTGCCTTAGTGGGTATCCACCTTTCTCTGAGCATAGGACTCAAGTGTC ACTGAAGGATCAGATCACCAGTGGAAAATACAACTTCATTCCTGAAGTCTGGGCAGAAGTCTCAGAGAAAGCTCTGGACCTTGTCAAGAAGTTGTTGGTA GTGGATCCAAAGGCACGTTTTACGACAGAAGAAGCCTTAAGACACCCGTGGCTTCAGGATGAAGACATGAAGAGAAAGTTTCAAGATCTTCTGTCTGAGG AAAATGAATCCACAGCTCTACCCCAGGTTCTAGCCCAGCCTTCTACTAGTCGAAAGCGGCCCCGTGAAGGGGAAGCCGAGGGTGCCGAGACCACAAAGCG CCCAGCTGTGTGTGCTGCTGTGTTGTGAACTCCGTGGTTTGAACACGAAAGAAATGTACCTTCTTTCACTCTGTCATCTTTCTTTTCTTTGAGTCTGTTT TTTTATAGTTTGTATTTTAATTATGGGAATAATTGCTTTTTCACAGTCACTGATGTACAATTAAAAACCTGATGGAACCTGGGCTTTGTGCTTCTGCTTG ATAATCGGTTCTTTAGTTGAATGGCTTTATTATTTATTTATTTGAGATGGAGTCTCACTCTGTTGCCCAGCCTGAATGAAGTGCAGTGGTGCAGGCTTGG CTCACTGCAACCTCTGCTTCCCAGGTTCAAGCGATTCTTGTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGTATGCACCACCATGCCTGTTAATTTTT ATATATTTTG

CHEK2.u.f.13 (forward primer)
CHEK2.u.r.25 (reverse primer)
Single exon skipping

Frame 0

VVMSRESDVEAQQSHGSSACSQPHGSVTQSQGS SSQSQGISSSSTSTMPNSSQSSHSSSGTLSSLET VSTQELYSIPEDQEPEDQEPEEPTPAPWARLWA LQDGFANLECVNDNYWFGRDKSCEYCFDEPLLK RTDKYRTYSKKHFRIFREENLSCPYRIWFNFCLF *SDCR*SVSLS*GIKR*IHHVKNSWKWCLWRGK AGFREENM*ESSHKDHQQKEVCYWFSKRGRPSS QC*NRNRNFEKAKSSLHHQD*KLF*CRRLLYCFG IDGRGRAV*QSGGE*TPERSYLQALFLPDALGC AVPS*KRYYTP*LKARECFTVISRRGLSYKDY* FWALQDFGRDLSHENLMWNPHLLGA*SSCFCWDC WV*PCCGLLEFRSYSFYLP*WVSTFL*A*DSSV TEGSDHQWKIQLHS*SLGRSLRESSGPCQEVVG SGSKGTFYDRRSLKTPVASG*RHEEKVSRSSV*G K*IHSSTPGSSPAFY*SKAAP*RGSRGCRDHKA PSCVCCCVVNSVV*TRKKCTFFHSVIFLFFESV FL*FVF*LWE*LLFHSH*CTIKNLMEPGLCASA* *SVL*LNGFIIYLFEMESHSVAQPE*SAVVQAW LTATSASQVQAILVPQPPE*LGLQVCTTMPVNF YIF

Frame 1

S*CLGSRMLRLSSLMAAVPVHSPMAALPSPKAP PHSPRAYPAPLPARCQTPASPLTPALGH*AP*R QCPLRNSILFLRTKNLRTKNLRSLPLPPGLDYGP FRMDLPILNV*MTTTGLGGTKAVNIALMNHC*K EQINTEHTARNTFGFSGRKICPVLTEYGLIFVF FDLTVDDQSVYPKALRDEYIMSKTLGSGACGEVK LAFERKTCKKVAIKIISKRKFAIGSAREADPAL NVETEIEILKKLNHPCIIKIKNFFDAEDYYIVL ELMEGGELFDKVVGNKRLKEATCKLYFYQMLLAV QYLHENGIIHRDLKPENVLLSSQEEDCLIKITD FGHSKILGETSLMRTLCGTPTYLAPEVLVSVGT AGYNRAVDCWSLGVILFICLSGYPPFSEHRTQVS LKDQITSGKYNFIPEVWAEVSEKALDLVKKLLV VDPKARFTTEEALRHPWLQDEDMKRKFQDLLSE ENESTALPQVLAQPSTSRKRPREGEAEGAETTKR PAVCAAVL*TPWFEHERNVPSFTLSSFFSLSLF FYSLYFNYGNNCFFTVTDVQLKT*WNLGFVLLL DNRFFS*MALLFIYLRWSLTLLPSLNEVQWCRLG SLQPLLPRFKRFLCLSLLSSWDYRYAPPCLLIF IYF

Frame 2

RDVSGVGC*GSAVSWQQCLFTAPWQRYPVPRLL LTVPGHIQLLYQHDAKLQPVLSLQLWDTELLRD SVHSGTLFYS*GPRT*GPRT*GAYPCPLGSIMG PSGWICQS*MCE*QLLVWEGQKL*ILL**TTAEK NR*IPNIQQETLSDFQGGKFVLSLQNMV*FLSF LI*L*MISQFILRH*EMNTSCQKLLEVVPVER* SWLSRGKHVRK*P*RSSAKGSLLLVQQERQTQLS MLKQK*KF*KS*IILASSRLKTFLMQKIIILFW N*WKGESCLTKWWGINA*KKLPASSIFTRCSWL CSTFMKTVLYTVT*SQRMFYCHLKKRTVL*RLLI LGTPRFWERPLS*EPYVEPPPTWRLKFLFLLGL LGITVLWTAGV*ELFFLSALVGIHLSLSIGLKC H*RIRSPVENTTSFLKSGQKSQRKLWTLSRSCW* WIQRHVLRQKKP*DTRGFRMKT*RESFKIFCLR KMNPQLYPRF*PSLLLVESGPVKGKPRVPRPQS AQLCVLLCCELRGLNTKEMYLLSLCHLSFL*VCF FIVCILIMGIIAFSQSLMYN*KPDGTWALCFCL IIGSLVEWLYYLFI*DGVSLCCPA*MKCSGAGL AHCNLCFPGSSDSCASAS*VAGITGMHHHAC*FL YIL