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Sequences

Sequence CHEK2.iAug05

GTCGTGATGTCTCGGGAGTCGGATGTTGAGGCTCAGCAGTCTCATGGCAGCAGTGCCTGTTCACAGCCCCATGGCAGCGTTACCCAGTCCCAAGGCTCCT
CCTCACAGTCCCAGGGCATATCCAGCTCCTCTACCAGCACGATGCCAAACTCCAGCCAGTCCTCTCACTCCAGCTCTGGGACACTGAGCTCCTTAGAGAC
AGTGTCCACTCAGGAACTCTATTCTATTCCTGAGGACCAAGAACCTGAGGACCAAGAACCTGAGGAGCCTACCCCTGCCCCCTGGGCTCGATTATGGGCC
CTTCAGGATGGATTTGCCAATCTTGAATGTGTGAATGACAACTACTGGTTTGGGAGGGACAAAAGCTGTGAATATTGCTTTGATGAACCACTGCTGAAAA
GAACAGATAAATACCGAACATACAGCAAGAAACACTTTCGGATTTTCAGGGAGGAAAATTTGTCCTGTCCTTACAGAATATGGTTTAATTTTTGTCTTTT
TTGATCTGACTGTAGATGATCAGTCAGTTTATCCTAAGGCATTAAGAGATGAATACATCATGTCAAAAACTCTTGGAAGTGGTGCCTGTGGAGAGGTAAA
GCTGGCTTTCGAGAGGAAAACATGTAAGAAAGTAGCCATAAAGATCATCAGCAAAAGGAAGTTTGCTATTGGTTCAGCAAGAGAGGCAGACCCAGCTCTC
AATGTTGAAACAGAAATAGAAATTTTGAAAAAGCTAAATCATCCTTGCATCATCAAGATTAAAAACTTTTTTGATGCAGAAGATTATTATATTGTTTTGG
AATTGATGGAAGGGGGAGAGCTGTTTGACAAAGTGGTGGGGAATAAACGCCTGAAAGAAGCTACCTGCAAGCTCTATTTTTACCAGATGCTCTTGGCTGT
GCAGTACCTTCATGAAAACGGTATTATACACCGTGACTTAAAGCCAGAGAATGTTTTACTGTCATCTCAAGAAGAGGACTGTCTTATAAAGATTACTGAT
TTTGGGCACTCCAAGATTTTGGGAGAGACCTCTCTCATGAGAACCTTATGTGGAACCCCCACCTACTTGGCGCCTGAAGTTCTTGTTTCTGTTGGGACTG
CTGGGTATAACCGTGCTGTGGACTGCTGGAGTTTAGGAGTTATTCTTTTTATCTGCCTTAGTGGGTATCCACCTTTCTCTGAGCATAGGACTCAAGTGTC
ACTGAAGGATCAGATCACCAGTGGAAAATACAACTTCATTCCTGAAGTCTGGGCAGAAGTCTCAGAGAAAGCTCTGGACCTTGTCAAGAAGTTGTTGGTA
GTGGATCCAAAGGCACGTTTTACGACAGAAGAAGCCTTAAGACACCCGTGGCTTCAGGATGAAGACATGAAGAGAAAGTTTCAAGATCTTCTGTCTGAGG
AAAATGAATCCACAGCTCTACCCCAGGTTCTAGCCCAGCCTTCTACTAGTCGAAAGCGGCCCCGTGAAGGGGAAGCCGAGGGTGCCGAGACCACAAAGCG
CCCAGCTGTGTGTGCTGCTGTGTTGTGAACTCCGTGGTTTGAACACGAAAGAAATGTACCTTCTTTCACTCTGTCATCTTTCTTTTCTTTGAGTCTGTTT
TTTTATAGTTTGTATTTTAATTATGGGAATAATTGCTTTTTCACAGTCACTGATGTACAATTAAAAACCTGATGGAACCTGGGCTTTGTGCTTCTGCTTG
ATAATCGGTTCTTTAGTTGAATGGCTTTATTATTTATTTATTTGAGATGGAGTCTCACTCTGTTGCCCAGCCTGAATGAAGTGCAGTGGTGCAGGCTTGG
CTCACTGCAACCTCTGCTTCCCAGGTTCAAGCGATTCTTGTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGTATGCACCACCATGCCTGTTAATTTTT
ATATATTTTG
  • CHEK2.u.f.13 (forward primer)
  • CHEK2.u.r.25 (reverse primer)
  • Mutually exclusive exon skipping

Frame 0

VVMSRESDVEAQQSHGSSACSQPHGSVTQSQGS
SSQSQGISSSSTSTMPNSSQSSHSSSGTLSSLET
VSTQELYSIPEDQEPEDQEPEEPTPAPWARLWA
LQDGFANLECVNDNYWFGRDKSCEYCFDEPLLK
RTDKYRTYSKKHFRIFREENLSCPYRIWFNFCLF
*SDCR*SVSLS*GIKR*IHHVKNSWKWCLWRGK
AGFREENM*ESSHKDHQQKEVCYWFSKRGRPSS
QC*NRNRNFEKAKSSLHHQD*KLF*CRRLLYCFG
IDGRGRAV*QSGGE*TPERSYLQALFLPDALGC
AVPS*KRYYTP*LKARECFTVISRRGLSYKDY*
FWALQDFGRDLSHENLMWNPHLLGA*SSCFCWDC
WV*PCCGLLEFRSYSFYLP*WVSTFL*A*DSSV
TEGSDHQWKIQLHS*SLGRSLRESSGPCQEVVG
SGSKGTFYDRRSLKTPVASG*RHEEKVSRSSV*G
K*IHSSTPGSSPAFY*SKAAP*RGSRGCRDHKA
PSCVCCCVVNSVV*TRKKCTFFHSVIFLFFESV
FL*FVF*LWE*LLFHSH*CTIKNLMEPGLCASA*
*SVL*LNGFIIYLFEMESHSVAQPE*SAVVQAW
LTATSASQVQAILVPQPPE*LGLQVCTTMPVNF
YIF

Frame 1

S*CLGSRMLRLSSLMAAVPVHSPMAALPSPKAP
PHSPRAYPAPLPARCQTPASPLTPALGH*AP*R
QCPLRNSILFLRTKNLRTKNLRSLPLPPGLDYGP
FRMDLPILNV*MTTTGLGGTKAVNIALMNHC*K
EQINTEHTARNTFGFSGRKICPVLTEYGLIFVF
FDLTVDDQSVYPKALRDEYIMSKTLGSGACGEVK
LAFERKTCKKVAIKIISKRKFAIGSAREADPAL
NVETEIEILKKLNHPCIIKIKNFFDAEDYYIVL
ELMEGGELFDKVVGNKRLKEATCKLYFYQMLLAV
QYLHENGIIHRDLKPENVLLSSQEEDCLIKITD
FGHSKILGETSLMRTLCGTPTYLAPEVLVSVGT
AGYNRAVDCWSLGVILFICLSGYPPFSEHRTQVS
LKDQITSGKYNFIPEVWAEVSEKALDLVKKLLV
VDPKARFTTEEALRHPWLQDEDMKRKFQDLLSE
ENESTALPQVLAQPSTSRKRPREGEAEGAETTKR
PAVCAAVL*TPWFEHERNVPSFTLSSFFSLSLF
FYSLYFNYGNNCFFTVTDVQLKT*WNLGFVLLL
DNRFFS*MALLFIYLRWSLTLLPSLNEVQWCRLG
SLQPLLPRFKRFLCLSLLSSWDYRYAPPCLLIF
IYF

Frame 2

RDVSGVGC*GSAVSWQQCLFTAPWQRYPVPRLL
LTVPGHIQLLYQHDAKLQPVLSLQLWDTELLRD
SVHSGTLFYS*GPRT*GPRT*GAYPCPLGSIMG
PSGWICQS*MCE*QLLVWEGQKL*ILL**TTAEK
NR*IPNIQQETLSDFQGGKFVLSLQNMV*FLSF
LI*L*MISQFILRH*EMNTSCQKLLEVVPVER*
SWLSRGKHVRK*P*RSSAKGSLLLVQQERQTQLS
MLKQK*KF*KS*IILASSRLKTFLMQKIIILFW
N*WKGESCLTKWWGINA*KKLPASSIFTRCSWL
CSTFMKTVLYTVT*SQRMFYCHLKKRTVL*RLLI
LGTPRFWERPLS*EPYVEPPPTWRLKFLFLLGL
LGITVLWTAGV*ELFFLSALVGIHLSLSIGLKC
H*RIRSPVENTTSFLKSGQKSQRKLWTLSRSCW*
WIQRHVLRQKKP*DTRGFRMKT*RESFKIFCLR
KMNPQLYPRF*PSLLLVESGPVKGKPRVPRPQS
AQLCVLLCCELRGLNTKEMYLLSLCHLSFL*VCF
FIVCILIMGIIAFSQSLMYN*KPDGTWALCFCL
IIGSLVEWLYYLFI*DGVSLCCPA*MKCSGAGL
AHCNLCFPGSSDSCASAS*VAGITGMHHHAC*FL
YIL

Sequence CHEK2.kAug05

CAAGTGATCCTCCCACCTCAGCTTCCCAAGTAGCTGGGACTACAGGTCGTGATGTCTCGGGAGTCGGATGTTGAGGCTCAGCAGTCTCATGGCAGCAGTG
CCTGTTCACAGCCCCATGGCAGCGTTACCCAGTCCCAAGGCTCCTCCTCACAGTCCCAGGGCATATCCAGCTCCTCTACCAGCACGATGCCAAACTCCAG
CCAGTCCTCTCACTCCAGCTCTGGGACACTGAGCTCCTTAGAGACAGTGTCCACTCAGGAACTCTATTCTATTCCTGAGGACCAAGAACCTGAGGACCAA
GAACCTGAGGAGCCTACCCCTGCCCCCTGGGCTCGATTATGGGCCCTTCAGGATGGATTTGCCAATCTTGAATGTGTGAATGACAACTACTGGTTTGGGA
GGGACAAAAGCTGTGAATATTGCTTTGATGAACCACTGCTGAAAAGAACAGATAAATACCGAACATACAGCAAGAAACACTTTCGGATTTTCAGGGAAGT
GGGTCCTAAAAACTCTTACATTGCATACATAGAAGATCACAGTGGCAATGGAACCTTTGTAAATACAGAGCTTGTAGGGAAAGGAAAACGCCGTCCTTTG
AATAACAATTCTGAAATTGCACTGTCACTAAGCAGAAATAAAGTGGTGCCTGTGGAGAGGTAAAGCTGGCTTTCGAGAGGAAAACATGTAAGAAAGTAGC
CATAAAGATCATCAGCAAAAGGAAGTTTGCTATTGGTTCAGCAAGAGAGGCAGACCCAGCTCTCAATGTTGAAACAGAAATAGAAATTTTGAAAAAGCTA
AATCATCCTTGCATCATCAAGATTAAAAACTTTTTTGATGCAGAAGATTATTATATTGTTTTGGAATTGATGGAAGGGGGAGAGCTGTTTGACAAAGTGG
TGGGGAATAAACGCCTGAAAGAAGCTACCTGCAAGCTCTATTTTTACCAGATGCTCTTGGCTGTGCAGTACCTTCATGAAAACGGTATTATACACCGTGA
CTTAAAGCCAGAGAATGTTTTACTGTCATCTCAAGAAGAGGACTGTCTTATAAAGATTACTGATTTTGGGCACTCCAAGATTTTGGGAGAGACCTCTCTC
ATGAGAACCTTATGTGGAACCCCCACCTACTTGGCGCCTGAAGTTCTTGTTTCTGTTGGGACTGCTGGGTATAACCGTGCTGTGGACTGCTGGAGTTTAG
GAGTTATTCTTTTTATCTGCCTTAGTGGGTATCCACCTTTCTCTGAGCATAGGACTCAAGTGTCACTGAAGGATCAGATCACCAGTGGAAAATACAACTT
CATTCCTGAAGTCTGGGCAGAAGTCTCAGAGAAAGCTCTGGACCTTGTCAAGAAGTTGTTGGTAGTGGATCCAAAGGCACGTTTTACGACAGAAGAAGCC
TTAAGACACCCGTGGCTTCAGGATGAAGACATGAAGAGAAAGTTTCAAGATCTTCTGTCTGAGGAAAATGAATCCACAGCTCTACCCCAGGTTCTAGCCC
AGCCTTCTACTAGTCGAAAGCGGCCCCGTGAAGGGGAAGCCGAGGGTGCCGAGACCACAAAGCGCCCAGCTGTGTGTGCTGCTGTGTTGTGAACTCCGTG
GTTTGAACACGAAAGAAATGTACCTTCTTTCACTCTGTCATCTTTCTTTTCTTTGAGTCTGTTTTTTTATAGTTTGTATTTTAATTATGGGAATAATTGC
TTTTTCACAGTCACTGATGTACAATTAAAAACCTGATGGAACCTGGGCTTTGTGCTTCTGCTTGATAATCGGTTCTTTAGTTGAATGGCTTTATTATTTA
TTTATTTGAGATGGAGTCTCACTCTGTTGCCCAGCCTGAATGAAGTGCAGTGGTGCAGGCTTGGCTCACTGCAACCTCTGCTTCCCAGGTTCAAGCGATT
CTTGTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGTATGCACCACCATGCCTGTTAATTTTTATATATTTTG
  • CHEK2.u.f.13 (forward primer)
  • CHEK2.u.r.25 (reverse primer)
  • Mutually exclusive exon skipping

Frame 0

QVILPPQLPK*LGLQVVMSRESDVEAQQSHGSS
ACSQPHGSVTQSQGSSSQSQGISSSSTSTMPNSS
QSSHSSSGTLSSLETVSTQELYSIPEDQEPEDQ
EPEEPTPAPWARLWALQDGFANLECVNDNYWFG
RDKSCEYCFDEPLLKRTDKYRTYSKKHFRIFREV
GPKNSYIAYIEDHSGNGTFVNTELVGKGKRRPL
NNNSEIALSLSRNKVVPVER*SWLSRGKHVRK*
P*RSSAKGSLLLVQQERQTQLSMLKQK*KF*KS*
IILASSRLKTFLMQKIIILFWN*WKGESCLTKW
WGINA*KKLPASSIFTRCSWLCSTFMKTVLYTV
T*SQRMFYCHLKKRTVL*RLLILGTPRFWERPLS
*EPYVEPPPTWRLKFLFLLGLLGITVLWTAGV*
ELFFLSALVGIHLSLSIGLKCH*RIRSPVENTT
SFLKSGQKSQRKLWTLSRSCW*WIQRHVLRQKKP
*DTRGFRMKT*RESFKIFCLRKMNPQLYPRF*P
SLLLVESGPVKGKPRVPRPQSAQLCVLLCCELR
GLNTKEMYLLSLCHLSFL*VCFFIVCILIMGIIA
FSQSLMYN*KPDGTWALCFCLIIGSLVEWLYYL
FI*DGVSLCCPA*MKCSGAGLAHCNLCFPGSSD
SCASAS*VAGITGMHHHAC*FLYIL

Frame 1

K*SSHLSFPSSWDYRS*CLGSRMLRLSSLMAAV
PVHSPMAALPSPKAPPHSPRAYPAPLPARCQTP
ASPLTPALGH*AP*RQCPLRNSILFLRTKNLRTK
NLRSLPLPPGLDYGPFRMDLPILNV*MTTTGLG
GTKAVNIALMNHC*KEQINTEHTARNTFGFSGK
WVLKTLTLHT*KITVAMEPL*IQSL*GKENAVL*
ITILKLHCH*AEIKWCLWRGKAGFREENM*ESS
HKDHQQKEVCYWFSKRGRPSSQC*NRNRNFEKA
KSSLHHQD*KLF*CRRLLYCFGIDGRGRAV*QSG
GE*TPERSYLQALFLPDALGCAVPS*KRYYTP*
LKARECFTVISRRGLSYKDY*FWALQDFGRDLS
HENLMWNPHLLGA*SSCFCWDCWV*PCCGLLEFR
SYSFYLP*WVSTFL*A*DSSVTEGSDHQWKIQL
HS*SLGRSLRESSGPCQEVVGSGSKGTFYDRRS
LKTPVASG*RHEEKVSRSSV*GK*IHSSTPGSSP
AFY*SKAAP*RGSRGCRDHKAPSCVCCCVVNSV
V*TRKKCTFFHSVIFLFFESVFL*FVF*LWE*L
LFHSH*CTIKNLMEPGLCASA**SVL*LNGFIIY
LFEMESHSVAQPE*SAVVQAWLTATSASQVQAI
LVPQPPE*LGLQVCTTMPVNFYIF

Frame 2

SDPPTSASQVAGTTGRDVSGVGC*GSAVSWQQC
LFTAPWQRYPVPRLLLTVPGHIQLLYQHDAKLQ
PVLSLQLWDTELLRDSVHSGTLFYS*GPRT*GP
RT*GAYPCPLGSIMGPSGWICQS*MCE*QLLVWE
GQKL*ILL**TTAEKNR*IPNIQQETLSDFQGS
GS*KLLHCIHRRSQWQWNLCKYRACRERKTPSF
E*QF*NCTVTKQK*SGACGEVKLAFERKTCKKVA
IKIISKRKFAIGSAREADPALNVETEIEILKKL
NHPCIIKIKNFFDAEDYYIVLELMEGGELFDKV
VGNKRLKEATCKLYFYQMLLAVQYLHENGIIHRD
LKPENVLLSSQEEDCLIKITDFGHSKILGETSL
MRTLCGTPTYLAPEVLVSVGTAGYNRAVDCWSL
GVILFICLSGYPPFSEHRTQVSLKDQITSGKYNF
IPEVWAEVSEKALDLVKKLLVVDPKARFTTEEA
LRHPWLQDEDMKRKFQDLLSEENESTALPQVLA
QPSTSRKRPREGEAEGAETTKRPAVCAAVL*TPW
FEHERNVPSFTLSSFFSLSLFFYSLYFNYGNNC
FFTVTDVQLKT*WNLGFVLLLDNRFFS*MALLF
IYLRWSLTLLPSLNEVQWCRLGSLQPLLPRFKRF
LCLSLLSSWDYRYAPPCLLIFIYF

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