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Sequences

Sequence CHEK2.lAug05

CHEK2.u.f.13 (forward primer)
CHEK2.u.r.25 (reverse primer)
Mutually exclusive exon skipping

Frame 0

VVMSRESDVEAQQSHGSSACSQPHGSVTQSQGS SSQSQGISSSSTSTMPNSSQSSHSSSGTLSSLET VSTQELYSIPEDQEPEDQEPEEPTPAPWARLWA LQDGFANLECVNDNYWFGRDKSCEYCFDEPLLK RTDKYRTYSKKHFRIFREENLSCPYRIWFNGACG EVKLAFERKTCKKVAIKIISKRKFAIGSAREAD PALNVETEIEILKKLNHPCIIKIKNFFDAEDYY IVLELMEGGELFDKVVGNKRLKEATCKLYFYQML LAVQYLHENGIIHRDLKPENVLLSSQEEDCLIK

Frame 1

S*CLGSRMLRLSSLMAAVPVHSPMAALPSPKAP PHSPRAYPAPLPARCQTPASPLTPALGH*AP*R QCPLRNSILFLRTKNLRTKNLRSLPLPPGLDYGP FRMDLPILNV*MTTTGLGGTKAVNIALMNHC*K EQINTEHTARNTFGFSGRKICPVLTEYGLMVPV ER*SWLSRGKHVRK*P*RSSAKGSLLLVQQERQT QLSMLKQK*KF*KS*IILASSRLKTFLMQKIII LFWN*WKGESCLTKWWGINA*KKLPASSIFTRC SWLCSTFMKTVLYTVT*SQRMFYCHLKKRTVL*

Frame 2

RDVSGVGC*GSAVSWQQCLFTAPWQRYPVPRLL LTVPGHIQLLYQHDAKLQPVLSLQLWDTELLRD SVHSGTLFYS*GPRT*GPRT*GAYPCPLGSIMG PSGWICQS*MCE*QLLVWEGQKL*ILL**TTAEK NR*IPNIQQETLSDFQGGKFVLSLQNMV*WCLW RGKAGFREENM*ESSHKDHQQKEVCYWFSKRGR PSSQC*NRNRNFEKAKSSLHHQD*KLF*CRRLLY CFGIDGRGRAV*QSGGE*TPERSYLQALFLPDA LGCAVPS*KRYYTP*LKARECFTVISRRGLSYK

Sequence CHEK2.aAug05

GCCTTCTCTTCCGCCAGCCAGATCCGTCCTCCCCTCTCGCAGGCCAGACCTCGACGTGTCTCTCGCCCGGGCCCGTTTCCCGAAACCGTCGCTCGTACCA GTCCATAAGGCTGAAGTAGTCTCGAGTGGGGTCAGGTGCCTGCAGCGCTCGGCACTGTGGGCAGAAGAACCTGTCCTCCCGCCCGGGGCCCCATGGGCCG CCGCAGTTCCAACAGCGGGGATAATTGCTTCCCGCCTGCGACGCAGCATCGCAGCTTAGCGGTCTCCTTCTGGGAACCCCTGTCGGCCAAAACCCCCACA CCCGGAGCAAAGCCCCGGCTCTCCCCCGCCACATCTGGCCGGCGGCCTATCTAGCCGTGGTCACTCGTGGGGAAAAGCAAAGAGAGCGTCTAACCAGACT AATGTTGCTGATTGGCTGGGGAGTCGAGGGGGCGGGATCACCCGAGGGGAACCCGGGTTCTAAGTTCCGCTCTCCCTTCTAAACTACAACTCCCAGGAGG CATTGAGGCGGCGCCTGACGGCCACATCTGCTGCTCCTCATTGGTCCGGCGGCAGGGGAGGGGGTTTTGATTGGCTGAGGGTGGAGTTTGTATCTGCAGG TTTAGCGCCACTCTGCTGGCTGAGGCTGCGGAGAGTGTGCGGCTCCAGGTGGGCTCACGCGGTCGTGATGTCTCGGGAGTCGGATGTTGAGGCTCAGCAG TCTCATGGCAGCAGTGCCTGTTCACAGCCCCATGGCAGCGTTACCCAGTCCCAAGGCTCCTCCTCACAGTCCCAGGGCATATCCAGCTCCTCTACCAGCA CGATGCCAAACTCCAGCCAGTCCTCTCACTCCAGCTCTGGGACACTGAGCTCCTTAGAGACAGTGTCCACTCAGGAACTCTATTCTATTCCTGAGGACCA AGAACCTGAGGACCAAGAACCTGAGGAGCCTACCCCTGCCCCCTGGGCTCGATTATGGGCCCTTCAGGATGGATTTGCCAATCTTGAGACAGAGTCTGGC CATGTTACCCAATCTGATCTTGAACTCCTGCTGTCATCTGATCCTCCTGCCTCAGCCTCCCAAAGTGCTGGGATAAGAGGTGTGAGGCACCATCCCCGGC CAGTTTGCAGTCTAAAATGTGTGAATGACAACTACTGGTTTGGGAGGGACAAAAGCTGTGAATATTGCTTTGATGAACCACTGCTGAAAAGAACAGATAA ATACCGAACATACAGCAAGAAACACTTTCGGATTTTCAGGGAAGTGGGTCCTAAAAACTCTTACATTGCATACATAGAAGATCACAGTGGCAATGGAACC TTTGTAAATACAGAGCTTGTAGGGAAAGGAAAACGCCGTCCTTTGAATAACAATTCTGAAATTGCACTGTCACTAAGCAGAAATAAAGTTTTTGTCTTTT TTGATCTGACTGTAGATGATCAGTCAGTTTATCCTAAGGCATTAAGAGATGAATACATCATGTCAAAAACTCTTGGAAGTGGTGCCTGTGGAGAGGTAAA GCTGGCTTTCGAGAGGAAAACATGTAAGAAAGTAGCCATAAAGATCATCAGCAAAAGGAAGTTTGCTATTGGTTCAGCAAGAGAGGCAGACCCAGCTCTC AATGTTGAAACAGAAATAGAAATTTTGAAAAAGCTAAATCATCCTTGCATCATCAAGATTAAAAACTTTTTTGATGCAGAAGATTATTATATTGTTTTGG AATTGATGGAAGGGGGAGAGCTGTTTGACAAAGTGGTGGGGAATAAACGCCTGAAAGAAGCTACCTGCAAGCTCTATTTTTACCAGATGCTCTTGGCTGT GCAGTACCTTCATGAAAACGGTATTATACACCGTGACTTAAAGCCAGAGAATGTTTTACTGTCATCTCAAGAAGAGGACTGTCTTATAAAGATTACTGAT TTTGGGCACTCCAAGATTTTGGGAGAGACCTCTCTCATGAGAACCTTATGTGGAACCCCCACCTACTTGGCGCCTGAAGTTCTTGTTTCTGTTGGGACTG CTGGGTATAACCGTGCTGTGGACTGCTGGAGTTTAGGAGTTATTCTTTTTATCTGCCTTAGTGGGTATCCACCTTTCTCTGAGCATAGGACTCAAGTGTC ACTGAAGGATCAGATCACCAGTGGAAAATACAACTTCATTCCTGAAGTCTGGGCAGAAGTCTCAGAGAAAGCTCTGGACCTTGTCAAGAAGTTGTTGGTA GTGGATCCAAAGGCACGTTTTACGACAGAAGAAGCCTTAAGACACCCGTGGCTTCAGGATGAAGACATGAAGAGAAAGTTTCAAGATCTTCTGTCTGAGG AAAATGAATCCACAGCTCTACCCCAGGTTCTAGCCCAGCCTTCTACTAGTCGAAAGCGGCCCCGTGAAGGGGAAGCCGAGGGTGCCGAGACCACAAAGCG CCCAGCTGTGTGTGCTGCTGTGTTGTGAACTCCGTGGTTTGAACACGAAAGAAATGTACCTTCTTTCACTCTGTCATCTTTCTTTTCTTTGAGTCTGTTT TTTTATAGTTTGTATTTTAATTATGGGAATAATTGCTTTTTCACAGTCACTGATGTACAATTAAAAACCTGATGGAACCTGGGCTTTGTGCTTCTGCTTG ATAATCGGTTCTTTAGTTGAATGGCTTTATTATTTATTTATTTGAGATGGAGTCTCACTCTGTTGCCCAGCCTGAATGAAGTGCAGTGGTGCAGGCTTGG CTCACTGCAACCTCTGCTTCCCAGGTTCAAGCGATTCTTGTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGTATGCACCACCATGCCTGTTAATTTTT ATATATTTTG

CHEK2.u.f.13 (forward primer)
CHEK2.u.r.25 (reverse primer)
Mutually exclusive exon skipping

Frame 0

AFSSASQIRPPLSQARPRRVSRPGPFPETVART SP*G*SSLEWGQVPAALGTVGRRTCPPARGPMGR RSSNSGDNCFPPATQHRSLAVSFWEPLSAKTPT PGAKPRLSPATSGRRPI*PWSLVGKSKESV*PD *CC*LAGESRGRDHPRGTRVLSSALPSKLQLPGG IEAAPDGHICCSSLVRRQGRGF*LAEGGVCICR FSATLLAEAAESVRLQVGSRGRDVSGVGC*GSA VSWQQCLFTAPWQRYPVPRLLLTVPGHIQLLYQH DAKLQPVLSLQLWDTELLRDSVHSGTLFYS*GP RT*GPRT*GAYPCPLGSIMGPSGWICQS*DRVW PCYPI*S*TPAVI*SSCLSLPKCWDKRCEAPSPA SLQSKMCE*QLLVWEGQKL*ILL**TTAEKNR* IPNIQQETLSDFQGSGS*KLLHCIHRRSQWQWN LCKYRACRERKTPSFE*QF*NCTVTKQK*SFCLF *SDCR*SVSLS*GIKR*IHHVKNSWKWCLWRGK AGFREENM*ESSHKDHQQKEVCYWFSKRGRPSS QC*NRNRNFEKAKSSLHHQD*KLF*CRRLLYCFG IDGRGRAV*QSGGE*TPERSYLQALFLPDALGC AVPS*KRYYTP*LKARECFTVISRRGLSYKDY* FWALQDFGRDLSHENLMWNPHLLGA*SSCFCWDC WV*PCCGLLEFRSYSFYLP*WVSTFL*A*DSSV TEGSDHQWKIQLHS*SLGRSLRESSGPCQEVVG SGSKGTFYDRRSLKTPVASG*RHEEKVSRSSV*G K*IHSSTPGSSPAFY*SKAAP*RGSRGCRDHKA PSCVCCCVVNSVV*TRKKCTFFHSVIFLFFESV FL*FVF*LWE*LLFHSH*CTIKNLMEPGLCASA* *SVL*LNGFIIYLFEMESHSVAQPE*SAVVQAW LTATSASQVQAILVPQPPE*LGLQVCTTMPVNF YIF

Frame 1

PSLPPARSVLPSRRPDLDVSLARARFPKPSLVP VHKAEVVSSGVRCLQRSALWAEEPVLPPGAPWA AAVPTAGIIASRLRRSIAA*RSPSGNPCRPKPPH PEQSPGSPPPHLAGGLSSRGHSWGKAKRASNQT NVADWLGSRGGGITRGEPGF*VPLSLLNYNSQE ALRRRLTATSAAPHWSGGRGGGFDWLRVEFVSAG LAPLCWLRLRRVCGSRWAHAVVMSRESDVEAQQ SHGSSACSQPHGSVTQSQGSSSQSQGISSSSTS TMPNSSQSSHSSSGTLSSLETVSTQELYSIPEDQ EPEDQEPEEPTPAPWARLWALQDGFANLETESG HVTQSDLELLLSSDPPASASQSAGIRGVRHHPR PVCSLKCVNDNYWFGRDKSCEYCFDEPLLKRTDK YRTYSKKHFRIFREVGPKNSYIAYIEDHSGNGT FVNTELVGKGKRRPLNNNSEIALSLSRNKVFVF FDLTVDDQSVYPKALRDEYIMSKTLGSGACGEVK LAFERKTCKKVAIKIISKRKFAIGSAREADPAL NVETEIEILKKLNHPCIIKIKNFFDAEDYYIVL ELMEGGELFDKVVGNKRLKEATCKLYFYQMLLAV QYLHENGIIHRDLKPENVLLSSQEEDCLIKITD FGHSKILGETSLMRTLCGTPTYLAPEVLVSVGT AGYNRAVDCWSLGVILFICLSGYPPFSEHRTQVS LKDQITSGKYNFIPEVWAEVSEKALDLVKKLLV VDPKARFTTEEALRHPWLQDEDMKRKFQDLLSE ENESTALPQVLAQPSTSRKRPREGEAEGAETTKR PAVCAAVL*TPWFEHERNVPSFTLSSFFSLSLF FYSLYFNYGNNCFFTVTDVQLKT*WNLGFVLLL DNRFFS*MALLFIYLRWSLTLLPSLNEVQWCRLG SLQPLLPRFKRFLCLSLLSSWDYRYAPPCLLIF IYF

Frame 2

LLFRQPDPSSPLAGQTSTCLSPGPVSRNRRSYQ SIRLK*SRVGSGACSARHCGQKNLSSRPGPHGP PQFQQRG*LLPACDAASQLSGLLLGTPVGQNPH TRSKAPALPRHIWPAAYLAVVTRGEKQRERLTRL MLLIGWGVEGAGSPEGNPGSKFRSPF*TTTPRR H*GGA*RPHLLLLIGPAAGEGVLIG*GWSLYLQ V*RHSAG*GCGECAAPGGLTRS*CLGSRMLRLSS LMAAVPVHSPMAALPSPKAPPHSPRAYPAPLPA RCQTPASPLTPALGH*AP*RQCPLRNSILFLRT KNLRTKNLRSLPLPPGLDYGPFRMDLPILRQSLA MLPNLILNSCCHLILLPQPPKVLG*EV*GTIPG QFAV*NV*MTTTGLGGTKAVNIALMNHC*KEQI NTEHTARNTFGFSGKWVLKTLTLHT*KITVAMEP L*IQSL*GKENAVL*ITILKLHCH*AEIKFLSF LI*L*MISQFILRH*EMNTSCQKLLEVVPVER* SWLSRGKHVRK*P*RSSAKGSLLLVQQERQTQLS MLKQK*KF*KS*IILASSRLKTFLMQKIIILFW N*WKGESCLTKWWGINA*KKLPASSIFTRCSWL CSTFMKTVLYTVT*SQRMFYCHLKKRTVL*RLLI LGTPRFWERPLS*EPYVEPPPTWRLKFLFLLGL LGITVLWTAGV*ELFFLSALVGIHLSLSIGLKC H*RIRSPVENTTSFLKSGQKSQRKLWTLSRSCW* WIQRHVLRQKKP*DTRGFRMKT*RESFKIFCLR KMNPQLYPRF*PSLLLVESGPVKGKPRVPRPQS AQLCVLLCCELRGLNTKEMYLLSLCHLSFL*VCF FIVCILIMGIIAFSQSLMYN*KPDGTWALCFCL IIGSLVEWLYYLFI*DGVSLCCPA*MKCSGAGL AHCNLCFPGSSDSCASAS*VAGITGMHHHAC*FL YIL

Sequence CHEK2.mAug05

GTCGTGATGTCTCGGGAGTCGGATGTTGAGGCTCAGCAGTCTCATGGCAGCAGTGCCTGTTCACAGCCCCATGGCAGCGTTACCCAGTCCCAAGGCTCCT CCTCACAGTCCCAGGGCATATCCAGCTCCTCTACCAGCACGATGCCAAACTCCAGCCAGTCCTCTCACTCCAGCTCTGGGACACTGAGCTCCTTAGAGAC AGTGTCCACTCAGGAACTCTATTCTATTCCTGAGGACCAAGAACCTGAGGACCAAGAACCTGAGGAGCCTACCCCTGCCCCCTGGGCTCGATTATGGGCC CTTCAGGATGGATTTGCCAATCTTGAATGTGTGAATGACAACTACTGGTTTGGGAGGGACAAAAGCTGTGAATATTGCTTTGATGAACCACTGCTGAAAA GAACAGATAAATACCGAACATACAGCAAGAAACACTTTCGGATTTTCAGGGAAGTGGGTCCTAAAAACTCTTACATTGCATACATAGAAGATCACAGTGG CAATGGAACCTTTGTAAATACAGAGCTTGTAGGGAAAGGAAAACGCCGTCCTTTGAATAACAATTCTGAAATTGCACTGTCACTAAGCAGAAATAAAGTT TTTGTCTTTTTTGATCTGACTGTAGATGATCAGTCAGTTTATCCTAAGGCATTAAGAGATGAATACATCATGTCAAAAACTCTTGGAAGTGGTGCCTGTG GAGAGGTAAAGCTGGCTTTCGAGAGGAAAACATGTAAGAAAGTAGCCATAAAGATCATCAGCAAAAGGAAGTTTGCTATTGGTTCAGCAAGAGAGGCAGA CCCAGCTCTCAATGTTGAAACAGAAATAGAAATTTTGAAAAAGCTAAATCATGATGGAAGGGGGAGAGCTGTTTGACAAAGTGGTGGGGAATAAACGCCT GAAAGAAGCTACCTGCAAGCTCTATTTTTACCAGATGCTCTTGGCTGTGCAGTACCTTCATGAAAACGGTATTATACACCGTGACTTAAAGCCAGAGAAT GTTTTACTGTCATCTCAAGAAGAGGACTGTCTTATAAAGATTACTGATTTTGGGCACTCCAAGATTTTGGGAGAGACCTCTCTCATGAGAACCTTATGTG GAACCCCCACCTACTTGGCGCCTGAAGTTCTTGTTTCTGTTGGGACTGCTGGGTATAACCGTGCTGTGGACTGCTGGAGTTTAGGAGTTATTCTTTTTAT CTGCCTTAGTGGGTATCCACCTTTCTCTGAGCATAGGACTCAAGTGTCACTGAAGGATCAGATCACCAGTGGAAAATACAACTTCATTCCTGAAGTCTGG GCAGAAGTCTCAGAGAAAGCTCTGGACCTTGTCAAGAAGTTGTTGGTAGTGGATCCAAAGGCACGTTTTACGACAGAAGAAGCCTTAAGACACCCGTGGC TTCAGGATGAAGACATGAAGAGAAAGTTTCAAGATCTTCTGTCTGAGGAAAATGAATCCACAGCTCTACCCCAGGTTCTAGCCCAGCCTTCTACTAGTCG AAAGCGGCCCCGTGAAGGGGAAGCCGAGGGTGCCGAGACCACAAAGCGCCCAGCTGTGTGTGCTGCTGTGTTGTGAACTCCGTGGTTTGAACACGAAAGA AATGTACCTTCTTTCACTCTGTCATCTTTCTTTTCTTTGAGTCTGTTTTTTTATAGTTTGTATTTTAATTATGGGAATAATTGCTTTTTCACAGTCACTG ATGTACAATTAAAAACCTGATGGAACCTGGGCTTTGTGCTTCTGCTTGATAATCGGTTCTTTAGTTGAATGGCTTTATTATTTATTTATTTGAGATGGAG TCTCACTCTGTTGCCCAGCCTGAATGAAGTGCAGTGGTGCAGGCTTGGCTCACTGCAACCTCTGCTTCCCAGGTTCAAGCGATTCTTGTGCCTCAGCCTC CTGAGTAGCTGGGATTACAGGTATGCACCACCATGCCTGTTAATTTTTATATATTTTG

CHEK2.u.f.13 (forward primer)
CHEK2.u.r.25 (reverse primer)
Mutually exclusive exon skipping

Frame 0

VVMSRESDVEAQQSHGSSACSQPHGSVTQSQGS SSQSQGISSSSTSTMPNSSQSSHSSSGTLSSLET VSTQELYSIPEDQEPEDQEPEEPTPAPWARLWA LQDGFANLECVNDNYWFGRDKSCEYCFDEPLLK RTDKYRTYSKKHFRIFREVGPKNSYIAYIEDHSG NGTFVNTELVGKGKRRPLNNNSEIALSLSRNKV FVFFDLTVDDQSVYPKALRDEYIMSKTLGSGAC GEVKLAFERKTCKKVAIKIISKRKFAIGSAREAD PALNVETEIEILKKLNHDGRGRAV*QSGGE*TP ERSYLQALFLPDALGCAVPS*KRYYTP*LKARE CFTVISRRGLSYKDY*FWALQDFGRDLSHENLMW NPHLLGA*SSCFCWDCWV*PCCGLLEFRSYSFY LP*WVSTFL*A*DSSVTEGSDHQWKIQLHS*SL GRSLRESSGPCQEVVGSGSKGTFYDRRSLKTPVA SG*RHEEKVSRSSV*GK*IHSSTPGSSPAFY*S KAAP*RGSRGCRDHKAPSCVCCCVVNSVV*TRK KCTFFHSVIFLFFESVFL*FVF*LWE*LLFHSH* CTIKNLMEPGLCASA**SVL*LNGFIIYLFEME SHSVAQPE*SAVVQAWLTATSASQVQAILVPQP PE*LGLQVCTTMPVNFYIF

Frame 1

S*CLGSRMLRLSSLMAAVPVHSPMAALPSPKAP PHSPRAYPAPLPARCQTPASPLTPALGH*AP*R QCPLRNSILFLRTKNLRTKNLRSLPLPPGLDYGP FRMDLPILNV*MTTTGLGGTKAVNIALMNHC*K EQINTEHTARNTFGFSGKWVLKTLTLHT*KITV AMEPL*IQSL*GKENAVL*ITILKLHCH*AEIKF LSFLI*L*MISQFILRH*EMNTSCQKLLEVVPV ER*SWLSRGKHVRK*P*RSSAKGSLLLVQQERQ TQLSMLKQK*KF*KS*IMMEGGELFDKVVGNKRL KEATCKLYFYQMLLAVQYLHENGIIHRDLKPEN VLLSSQEEDCLIKITDFGHSKILGETSLMRTLC GTPTYLAPEVLVSVGTAGYNRAVDCWSLGVILFI CLSGYPPFSEHRTQVSLKDQITSGKYNFIPEVW AEVSEKALDLVKKLLVVDPKARFTTEEALRHPW LQDEDMKRKFQDLLSEENESTALPQVLAQPSTSR KRPREGEAEGAETTKRPAVCAAVL*TPWFEHER NVPSFTLSSFFSLSLFFYSLYFNYGNNCFFTVT DVQLKT*WNLGFVLLLDNRFFS*MALLFIYLRWS LTLLPSLNEVQWCRLGSLQPLLPRFKRFLCLSL LSSWDYRYAPPCLLIFIYF

Frame 2

RDVSGVGC*GSAVSWQQCLFTAPWQRYPVPRLL LTVPGHIQLLYQHDAKLQPVLSLQLWDTELLRD SVHSGTLFYS*GPRT*GPRT*GAYPCPLGSIMG PSGWICQS*MCE*QLLVWEGQKL*ILL**TTAEK NR*IPNIQQETLSDFQGSGS*KLLHCIHRRSQW QWNLCKYRACRERKTPSFE*QF*NCTVTKQK*S FCLF*SDCR*SVSLS*GIKR*IHHVKNSWKWCLW RGKAGFREENM*ESSHKDHQQKEVCYWFSKRGR PSSQC*NRNRNFEKAKS*WKGESCLTKWWGINA *KKLPASSIFTRCSWLCSTFMKTVLYTVT*SQRM FYCHLKKRTVL*RLLILGTPRFWERPLS*EPYV EPPPTWRLKFLFLLGLLGITVLWTAGV*ELFFL SALVGIHLSLSIGLKCH*RIRSPVENTTSFLKSG QKSQRKLWTLSRSCW*WIQRHVLRQKKP*DTRG FRMKT*RESFKIFCLRKMNPQLYPRF*PSLLLV ESGPVKGKPRVPRPQSAQLCVLLCCELRGLNTKE MYLLSLCHLSFL*VCFFIVCILIMGIIAFSQSL MYN*KPDGTWALCFCLIIGSLVEWLYYLFI*DG VSLCCPA*MKCSGAGLAHCNLCFPGSSDSCASAS *VAGITGMHHHAC*FLYIL

Sequence CHEK2.gAug05

GCCTTCTCTTCCGCCAGCCAGATCCGTCCTCCCCTCTCGCAGGCCAGACCTCGACGTGTCTCTCGCCCGGGCCCGTTTCCCGAAACCGTCGCTCGTACCA GTCCATAAGGCTGAAGTAGTCTCGAGTGGGGTCAGGTGCCTGCAGCGCTCGGCACTGTGGGCAGAAGAACCTGTCCTCCCGCCCGGGGCCCCATGGGCCG CCGCAGTTCCAACAGCGGGGATAATTGCTTCCCGCCTGCGACGCAGCATCGCAGCTTAGCGGTCTCCTTCTGGGAACCCCTGTCGGCCAAAACCCCCACA CCCGGAGCAAAGCCCCGGCTCTCCCCCGCCACATCTGGCCGGCGGCCTATCTAGCCGTGGTCACTCGTGGGGAAAAGCAAAGAGAGCGTCTAACCAGACT AATGTTGCTGATTGGCTGGGGAGTCGAGGGGGCGGGATCACCCGAGGGGAACCCGGGTTCTAAGTTCCGCTCTCCCTTCTAAACTACAACTCCCAGGAGG CATTGAGGCGGCGCCTGACGGCCACATCTGCTGCTCCTCATTGGTCCGGCGGCAGGGGAGGGGGTTTTGATTGGCTGAGGGTGGAGTTTGTATCTGCAGG TTTAGCGCCACTCTGCTGGCTGAGGCTGCGGAGAGTGTGCGGCTCCAGGTGGGCTCACGCGATACTGGATTTCACCATGGTGCCCAGGCTGGTCTTGAAT TCCTGGGCTCAAGCAATCTACCTGCGTTGGCCTCCCAAAGTGCTGGGATTACAGACATGAACCACCACACCTGGCAGAACCTTTGTGAAAGATCTCAATT TAATATGGAAATAAAAAAATTTGTATGCTGAGGCTGCTAACGTCTATGGTCGTGATGTCTCGGGAGTCGGATGTTGAGGCTCAGCAGTCTCATGGCAGCA GTGCCTGTTCACAGCCCCATGGCAGCGTTACCCAGTCCCAAGGCTCCTCCTCACAGTCCCAGGGCATATCCAGCTCCTCTACCAGCACGATGCCAAACTC CAGCCAGTCCTCTCACTCCAGCTCTGGGACACTGAGCTCCTTAGAGACAGTGTCCACTCAGGAACTCTATTCTATTCCTGAGGACCAAGAACCTGAGGAC CAAGAACCTGAGGAGCCTACCCCTGCCCCCTGGGCTCGATTATGGGCCCTTCAGGATGGATTTGCCAATCTTGAATGTGTGAATGACAACTACTGGTTTG GGAGGGACAAAAGCTGTGAATATTGCTTTGATGAACCACTGCTGAAAAGAACAGATAAATACCGAACATACAGCAAGAAACACTTTCGGATTTTCAGGGA AGTGGGTCCTAAAAACTCTTACATTGCATACATAGAAGATCACAGTGGCAATGGAACCTTTGTAAATACAGAGCTTGTAGGGAAAGGAAAACGCCGTCCT TTGAATAACAATTCTGAAATTGCACTGTCACTAAGCAGAAATAAAGTTTTTGTCTTTTTTGATCTGACTGTAGATGATCAGTCAGTTTATCCTAAGGCAT TAAGAGATGAATACATCATGTCAAAAACTCTTGGAAGTGGTGCCTGTGGAGAGGTAAAGCTGGCTTTCGAGAGGAAAACATGTAAGAAAGTAGCCATAAA GATCATCAGCAAAAGGAAGTTTGCTATTGGTTCAGCAAGAGAGGCAGACCCAGCTCTCAATGTTGAAACAGAAATAGAAATTTTGAAAAAGCTAAATCAT CCTTGCATCATCAAGATTAAAAACTTTTTTGATGCAGAAGATTATTATATTGTTTTGGAATTGATGGAAGGGGGAGAGCTGTTTGACAAAGTGGTGGGGA ATAAACGCCTGAAAGAAGCTACCTGCAAGCTCTATTTTTACCAGATGCTCTTGGCTGTGCAGGATGAAGACATGAAGAGAAAGTTTCAAGATCTTCTGTC TGAGGAAAATGAATCCACAGCTCTACCCCAGGTTCTAGCCCAGCCTTCTACTAGTCGAAAGCGGCCCCGTGAAGGGGAAGCCGAGGGTGCCGAGACCACA AAGCGCCCAGCTGTGTGTGCTGCTGTGTTGTGAACTCCGTGGTTTGAACACGAAAGAAATGTACCTTCTTTCACTCTGTCATCTTTCTTTTCTTTGAGTC TGTTTTTTTATAGTTTGTATTTTAATTATGGGAATAATTGCTTTTTCACAGTCACTGATGTACAATTAAAAACCTGATGGAACCTGGGCTTTGTGCTTCT GCTTGATAATCGGTTCTTTAGTTGAATGGCTTTATTATTTATTTATTTGAGATGGAGTCTCACTCTGTTGCCCAGCCTGAATGAAGTGCAGTGGTGCAGG CTTGGCTCACTGCAACCTCTGCTTCCCAGGTTCAAGCGATTCTTGTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGTATGCACCACCATGCCTGTTAA TTTTTATATATTTTG

CHEK2.u.f.13 (forward primer)
CHEK2.u.r.25 (reverse primer)
Mutually exclusive exon skipping

Frame 0

AFSSASQIRPPLSQARPRRVSRPGPFPETVART SP*G*SSLEWGQVPAALGTVGRRTCPPARGPMGR RSSNSGDNCFPPATQHRSLAVSFWEPLSAKTPT PGAKPRLSPATSGRRPI*PWSLVGKSKESV*PD *CC*LAGESRGRDHPRGTRVLSSALPSKLQLPGG IEAAPDGHICCSSLVRRQGRGF*LAEGGVCICR FSATLLAEAAESVRLQVGSRDTGFHHGAQAGLE FLGSSNLPALASQSAGITDMNHHTWQNLCERSQF NMEIKKFVC*GC*RLWS*CLGSRMLRLSSLMAA VPVHSPMAALPSPKAPPHSPRAYPAPLPARCQT PASPLTPALGH*AP*RQCPLRNSILFLRTKNLRT KNLRSLPLPPGLDYGPFRMDLPILNV*MTTTGL GGTKAVNIALMNHC*KEQINTEHTARNTFGFSG KWVLKTLTLHT*KITVAMEPL*IQSL*GKENAVL *ITILKLHCH*AEIKFLSFLI*L*MISQFILRH *EMNTSCQKLLEVVPVER*SWLSRGKHVRK*P* RSSAKGSLLLVQQERQTQLSMLKQK*KF*KS*II LASSRLKTFLMQKIIILFWN*WKGESCLTKWWG INA*KKLPASSIFTRCSWLCRMKT*RESFKIFC LRKMNPQLYPRF*PSLLLVESGPVKGKPRVPRPQ SAQLCVLLCCELRGLNTKEMYLLSLCHLSFL*V CFFIVCILIMGIIAFSQSLMYN*KPDGTWALCF CLIIGSLVEWLYYLFI*DGVSLCCPA*MKCSGAG LAHCNLCFPGSSDSCASAS*VAGITGMHHHAC* FLYIL

Frame 1

PSLPPARSVLPSRRPDLDVSLARARFPKPSLVP VHKAEVVSSGVRCLQRSALWAEEPVLPPGAPWA AAVPTAGIIASRLRRSIAA*RSPSGNPCRPKPPH PEQSPGSPPPHLAGGLSSRGHSWGKAKRASNQT NVADWLGSRGGGITRGEPGF*VPLSLLNYNSQE ALRRRLTATSAAPHWSGGRGGGFDWLRVEFVSAG LAPLCWLRLRRVCGSRWAHAILDFTMVPRLVLN SWAQAIYLRWPPKVLGLQT*TTTPGRTFVKDLN LIWK*KNLYAEAANVYGRDVSGVGC*GSAVSWQQ CLFTAPWQRYPVPRLLLTVPGHIQLLYQHDAKL QPVLSLQLWDTELLRDSVHSGTLFYS*GPRT*G PRT*GAYPCPLGSIMGPSGWICQS*MCE*QLLVW EGQKL*ILL**TTAEKNR*IPNIQQETLSDFQG SGS*KLLHCIHRRSQWQWNLCKYRACRERKTPS FE*QF*NCTVTKQK*SFCLF*SDCR*SVSLS*GI KR*IHHVKNSWKWCLWRGKAGFREENM*ESSHK DHQQKEVCYWFSKRGRPSSQC*NRNRNFEKAKS SLHHQD*KLF*CRRLLYCFGIDGRGRAV*QSGGE *TPERSYLQALFLPDALGCAG*RHEEKVSRSSV *GK*IHSSTPGSSPAFY*SKAAP*RGSRGCRDH KAPSCVCCCVVNSVV*TRKKCTFFHSVIFLFFES VFL*FVF*LWE*LLFHSH*CTIKNLMEPGLCAS A**SVL*LNGFIIYLFEMESHSVAQPE*SAVVQ AWLTATSASQVQAILVPQPPE*LGLQVCTTMPVN FYIF

Frame 2

LLFRQPDPSSPLAGQTSTCLSPGPVSRNRRSYQ SIRLK*SRVGSGACSARHCGQKNLSSRPGPHGP PQFQQRG*LLPACDAASQLSGLLLGTPVGQNPH TRSKAPALPRHIWPAAYLAVVTRGEKQRERLTRL MLLIGWGVEGAGSPEGNPGSKFRSPF*TTTPRR H*GGA*RPHLLLLIGPAAGEGVLIG*GWSLYLQ V*RHSAG*GCGECAAPGGLTRYWISPWCPGWS*I PGLKQSTCVGLPKCWDYRHEPPHLAEPL*KISI *YGNKKICMLRLLTSMVVMSRESDVEAQQSHGS SACSQPHGSVTQSQGSSSQSQGISSSSTSTMPNS SQSSHSSSGTLSSLETVSTQELYSIPEDQEPED QEPEEPTPAPWARLWALQDGFANLECVNDNYWF GRDKSCEYCFDEPLLKRTDKYRTYSKKHFRIFRE VGPKNSYIAYIEDHSGNGTFVNTELVGKGKRRP LNNNSEIALSLSRNKVFVFFDLTVDDQSVYPKA LRDEYIMSKTLGSGACGEVKLAFERKTCKKVAIK IISKRKFAIGSAREADPALNVETEIEILKKLNH PCIIKIKNFFDAEDYYIVLELMEGGELFDKVVG NKRLKEATCKLYFYQMLLAVQDEDMKRKFQDLLS EENESTALPQVLAQPSTSRKRPREGEAEGAETT KRPAVCAAVL*TPWFEHERNVPSFTLSSFFSLS LFFYSLYFNYGNNCFFTVTDVQLKT*WNLGFVLL LDNRFFS*MALLFIYLRWSLTLLPSLNEVQWCR LGSLQPLLPRFKRFLCLSLLSSWDYRYAPPCLL IFIYF

Sequence CHEK2.eAug05

GCCTTCTCTTCCGCCAGCCAGATCCGTCCTCCCCTCTCGCAGGCCAGACCTCGACGTGTCTCTCGCCCGGGCCCGTTTCCCGAAACCGTCGCTCGTACCA GTCCATAAGGCTGAAGTAGTCTCGAGTGGGGTCAGGTGCCTGCAGCGCTCGGCACTGTGGGCAGAAGAACCTGTCCTCCCGCCCGGGGCCCCATGGGCCG CCGCAGTTCCAACAGCGGGGATAATTGCTTCCCGCCTGCGACGCAGCATCGCAGCTTAGCGGTCTCCTTCTGGGAACCCCTGTCGGCCAAAACCCCCACA CCCGGAGCAAAGCCCCGGCTCTCCCCCGCCACATCTGGCCGGCGGCCTATCTAGCCGTGGTCACTCGTGGGGAAAAGCAAAGAGAGCGTCTAACCAGACT AATGTTGCTGATTGGCTGGGGAGTCGAGGGGGCGGGATCACCCGAGGGGAACCCGGGTTCTAAGTTCCGCTCTCCCTTCTAAACTACAACTCCCAGGAGG CATTGAGGCGGCGCCTGACGGCCACATCTGCTGCTCCTCATTGGTCCGGCGGCAGGGGAGGGGGTTTTGATTGGCTGAGGGTGGAGTTTGTATCTGCAGG TTTAGCGCCACTCTGCTGGCTGAGGCTGCGGAGAGTGTGCGGCTCCAGGTGGGCTCACGCGGTCGTGATGTCTCGGGAGTCGGATGTTGAGGCTCAGCAG TCTCATGGCAGCAGTGCCTGTTCACAGCCCCATGGCAGCGTTACCCAGTCCCAAGGCTCCTCCTCACAGTCCCAGGGCATATCCAGCTCCTCTACCAGCA CGATGCCAAACTCCAGCCAGTCCTCTCACTCCAGCTCTGGGACACTGAGCTCCTTAGAGACAGTGTCCACTCAGGAACTCTATTCTATTCCTGAGGACCA AGAACCTGAGGACCAAGAACCTGAGGAGCCTACCCCTGCCCCCTGGGCTCGATTATGGGCCCTTCAGGATGGATTTGCCAATCTTGAATGTGTGAATGAC AACTACTGGTTTGGGAGGGACAAAAGCTGTGAATATTGCTTTGATGAACCACTGCTGAAAAGAACAGATAAATACCGAACATACAGCAAGAAACACTTTC GGATTTTCAGGGAAGTGGGTCCTAAAAACTCTTACATTGCATACATAGAAGATCACAGTGGCAATGGAACCTTTGTAAATACAGAGCTTGTAGGGAAAGG AAAACGCCGTCCTTTGAATAACAATTCTGAAATTGCACTGTCACTAAGCAGAAATAAAGTTTTTGTCTTTTTTGATCTGACTGTAGATGATCAGTCAGTT TATCCTAAGGCATTAAGAGATGAATACATCATGTCAAAAACTCTTGGAAGTGGTGCCTGTGGAGAGGTAAAGCTGGCTTTCGAGAGGAAAACATGTAAGA AAGTAGCCATAAAGATCATCAGCAAAAGGAAGTTTGCTATTGGTTCAGCAAGAGAGGCAGACCCAGCTCTCAATGTTGAAACAGAAATAGAAATTTTGAA AAAGCTAAATCATCCTTGCATCATCAAGATTAAAAACTTTTTTGATGCAGAAGATTATTATATTGTTTTGGAATTGATGGAAGGGGGAGAGCTGTTTGAC AAAGTGGTGGGGAATAAACGCCTGAAAGAAGCTACCTGCAAGCTCTATTTTTACCAGATGCTCTTGGCTGTGCAGATTACTGATTTTGGGCACTCCAAGA TTTTGGGAGAGACCTCTCTCATGAGAACCTTATGTGGAACCCCCACCTACTTGGCGCCTGAAGTTCTTGTTTCTGTTGGGACTGCTGGGTATAACCGTGC TGTGGACTGCTGGAGTTTAGGAGTTATTCTTTTTATCTGCCTTAGTGGGTATCCACCTTTCTCTGAGCATAGGACTCAAGTGTCACTGAAGGATCAGATC ACCAGTGGAAAATACAACTTCATTCCTGAAGTCTGGGCAGAAGTCTCAGAGAAAGCTCTGGACCTTGTCAAGAAGTTGTTGGTAGTGGATCCAAAGGCAC GTTTTACGACAGAAGAAGCCTTAAGACACCCGTGGCTTCAGGATGAAGACATGAAGAGAAAGTTTCAAGATCTTCTGTCTGAGGAAAATGAATCCACAGC TCTACCCCAGGTTCTAGCCCAGCCTTCTACTAGTCGAAAGCGGCCCCGTGAAGGGGAAGCCGAGGGTGCCGAGACCACAAAGCGCCCAGCTGTGTGTGCT GCTGTGTTGTGAACTCCGTGGTTTGAACACGAAAGAAATGTACCTTCTTTCACTCTGTCATCTTTCTTTTCTTTGAGTCTGTTTTTTTATAGTTTGTATT TTAATTATGGGAATAATTGCTTTTTCACAGTCACTGATGTACAATTAAAAACCTGATGGAACCTGGGCTTTGTGCTTCTGCTTGATAATCGGTTCTTTAG TTGAATGGCTTTATTATTTATTTATTTGAGATGGAGTCTCACTCTGTTGCCCAGCCTGAATGAAGTGCAGTGGTGCAGGCTTGGCTCACTGCAACCTCTG CTTCCCAGGTTCAAGCGATTCTTGTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGTATGCACCACCATGCCTGTTAATTTTTATATATTTTG

CHEK2.u.f.13 (forward primer)
CHEK2.u.r.25 (reverse primer)
Mutually exclusive exon skipping

Frame 0

AFSSASQIRPPLSQARPRRVSRPGPFPETVART SP*G*SSLEWGQVPAALGTVGRRTCPPARGPMGR RSSNSGDNCFPPATQHRSLAVSFWEPLSAKTPT PGAKPRLSPATSGRRPI*PWSLVGKSKESV*PD *CC*LAGESRGRDHPRGTRVLSSALPSKLQLPGG IEAAPDGHICCSSLVRRQGRGF*LAEGGVCICR FSATLLAEAAESVRLQVGSRGRDVSGVGC*GSA VSWQQCLFTAPWQRYPVPRLLLTVPGHIQLLYQH DAKLQPVLSLQLWDTELLRDSVHSGTLFYS*GP RT*GPRT*GAYPCPLGSIMGPSGWICQS*MCE* QLLVWEGQKL*ILL**TTAEKNR*IPNIQQETLS DFQGSGS*KLLHCIHRRSQWQWNLCKYRACRER KTPSFE*QF*NCTVTKQK*SFCLF*SDCR*SVS LS*GIKR*IHHVKNSWKWCLWRGKAGFREENM*E SSHKDHQQKEVCYWFSKRGRPSSQC*NRNRNFE KAKSSLHHQD*KLF*CRRLLYCFGIDGRGRAV* QSGGE*TPERSYLQALFLPDALGCADY*FWALQD FGRDLSHENLMWNPHLLGA*SSCFCWDCWV*PC CGLLEFRSYSFYLP*WVSTFL*A*DSSVTEGSD HQWKIQLHS*SLGRSLRESSGPCQEVVGSGSKGT FYDRRSLKTPVASG*RHEEKVSRSSV*GK*IHS STPGSSPAFY*SKAAP*RGSRGCRDHKAPSCVC CCVVNSVV*TRKKCTFFHSVIFLFFESVFL*FVF *LWE*LLFHSH*CTIKNLMEPGLCASA**SVL* LNGFIIYLFEMESHSVAQPE*SAVVQAWLTATS ASQVQAILVPQPPE*LGLQVCTTMPVNFYIF

Frame 1

PSLPPARSVLPSRRPDLDVSLARARFPKPSLVP VHKAEVVSSGVRCLQRSALWAEEPVLPPGAPWA AAVPTAGIIASRLRRSIAA*RSPSGNPCRPKPPH PEQSPGSPPPHLAGGLSSRGHSWGKAKRASNQT NVADWLGSRGGGITRGEPGF*VPLSLLNYNSQE ALRRRLTATSAAPHWSGGRGGGFDWLRVEFVSAG LAPLCWLRLRRVCGSRWAHAVVMSRESDVEAQQ SHGSSACSQPHGSVTQSQGSSSQSQGISSSSTS TMPNSSQSSHSSSGTLSSLETVSTQELYSIPEDQ EPEDQEPEEPTPAPWARLWALQDGFANLECVND NYWFGRDKSCEYCFDEPLLKRTDKYRTYSKKHF RIFREVGPKNSYIAYIEDHSGNGTFVNTELVGKG KRRPLNNNSEIALSLSRNKVFVFFDLTVDDQSV YPKALRDEYIMSKTLGSGACGEVKLAFERKTCK KVAIKIISKRKFAIGSAREADPALNVETEIEILK KLNHPCIIKIKNFFDAEDYYIVLELMEGGELFD KVVGNKRLKEATCKLYFYQMLLAVQITDFGHSK ILGETSLMRTLCGTPTYLAPEVLVSVGTAGYNRA VDCWSLGVILFICLSGYPPFSEHRTQVSLKDQI TSGKYNFIPEVWAEVSEKALDLVKKLLVVDPKA RFTTEEALRHPWLQDEDMKRKFQDLLSEENESTA LPQVLAQPSTSRKRPREGEAEGAETTKRPAVCA AVL*TPWFEHERNVPSFTLSSFFSLSLFFYSLY FNYGNNCFFTVTDVQLKT*WNLGFVLLLDNRFFS *MALLFIYLRWSLTLLPSLNEVQWCRLGSLQPL LPRFKRFLCLSLLSSWDYRYAPPCLLIFIYF

Frame 2

LLFRQPDPSSPLAGQTSTCLSPGPVSRNRRSYQ SIRLK*SRVGSGACSARHCGQKNLSSRPGPHGP PQFQQRG*LLPACDAASQLSGLLLGTPVGQNPH TRSKAPALPRHIWPAAYLAVVTRGEKQRERLTRL MLLIGWGVEGAGSPEGNPGSKFRSPF*TTTPRR H*GGA*RPHLLLLIGPAAGEGVLIG*GWSLYLQ V*RHSAG*GCGECAAPGGLTRS*CLGSRMLRLSS LMAAVPVHSPMAALPSPKAPPHSPRAYPAPLPA RCQTPASPLTPALGH*AP*RQCPLRNSILFLRT KNLRTKNLRSLPLPPGLDYGPFRMDLPILNV*MT TTGLGGTKAVNIALMNHC*KEQINTEHTARNTF GFSGKWVLKTLTLHT*KITVAMEPL*IQSL*GK ENAVL*ITILKLHCH*AEIKFLSFLI*L*MISQF ILRH*EMNTSCQKLLEVVPVER*SWLSRGKHVR K*P*RSSAKGSLLLVQQERQTQLSMLKQK*KF* KS*IILASSRLKTFLMQKIIILFWN*WKGESCLT KWWGINA*KKLPASSIFTRCSWLCRLLILGTPR FWERPLS*EPYVEPPPTWRLKFLFLLGLLGITV LWTAGV*ELFFLSALVGIHLSLSIGLKCH*RIRS PVENTTSFLKSGQKSQRKLWTLSRSCW*WIQRH VLRQKKP*DTRGFRMKT*RESFKIFCLRKMNPQ LYPRF*PSLLLVESGPVKGKPRVPRPQSAQLCVL LCCELRGLNTKEMYLLSLCHLSFL*VCFFIVCI LIMGIIAFSQSLMYN*KPDGTWALCFCLIIGSL VEWLYYLFI*DGVSLCCPA*MKCSGAGLAHCNLC FPGSSDSCASAS*VAGITGMHHHAC*FLYIL

Sequence CHEK2.dAug05

GCCTTCTCTTCCGCCAGCCAGATCCGTCCTCCCCTCTCGCAGGCCAGACCTCGACGTGTCTCTCGCCCGGGCCCGTTTCCCGAAACCGTCGCTCGTACCA GTCCATAAGGCTGAAGTAGTCTCGAGTGGGGTCAGGTGCCTGCAGCGCTCGGCACTGTGGGCAGAAGAACCTGTCCTCCCGCCCGGGGCCCCATGGGCCG CCGCAGTTCCAACAGCGGGGATAATTGCTTCCCGCCTGCGACGCAGCATCGCAGCTTAGCGGTCTCCTTCTGGGAACCCCTGTCGGCCAAAACCCCCACA CCCGGAGCAAAGCCCCGGCTCTCCCCCGCCACATCTGGCCGGCGGCCTATCTAGCCGTGGTCACTCGTGGGGAAAAGCAAAGAGAGCGTCTAACCAGACT AATGTTGCTGATTGGCTGGGGAGTCGAGGGGGCGGGATCACCCGAGGGGAACCCGGGTTCTAAGTTCCGCTCTCCCTTCTAAACTACAACTCCCAGGAGG CATTGAGGCGGCGCCTGACGGCCACATCTGCTGCTCCTCATTGGTCCGGCGGCAGGGGAGGGGGTTTTGATTGGCTGAGGGTGGAGTTTGTATCTGCAGG TTTAGCGCCACTCTGCTGGCTGAGGCTGCGGAGAGTGTGCGGCTCCAGGTGGGCTCACGCGGTCGTGATGTCTCGGGAGTCGGATGTTGAGGCTCAGCAG TCTCATGGCAGCAGTGCCTGTTCACAGCCCCATGGCAGCGTTACCCAGTCCCAAGGCTCCTCCTCACAGTCCCAGGGCATATCCAGCTCCTCTACCAGCA CGATGCCAAACTCCAGCCAGTCCTCTCACTCCAGCTCTGGGACACTGAGCTCCTTAGAGACAGTGTCCACTCAGGAACTCTATTCTATTCCTGAGGACCA AGAACCTGAGGACCAAGAACCTGAGGAGCCTACCCCTGCCCCCTGGGCTCGATTATGGGCCCTTCAGGATGGATTTGCCAATCTTGAATGTGTGAATGAC AACTACTGGTTTGGGAGGGACAAAAGCTGTGAATATTGCTTTGATGAACCACTGCTGAAAAGAACAGATAAATACCGAACATACAGCAAGAAACACTTTC GGATTTTCAGGGAAGTGGGTCCTAAAAACTCTTACATTGCATACATAGAAGATCACAGTGGCAATGGAACCTTTGTAAATACAGAGCTTGTAGGGAAAGG AAAACGCCGTCCTTTGAATAACAATTCTGAAATTGCACTGTCACTAAGCAGAAATAAAGTTTTTGTCTTTTTTGATCTGACTGTAGATGATCAGTCAGTT TATCCTAAGGCATTAAGAGATGAATACATCATGTCAAAAACTCTTGGAAGTGGTGCCTGTGGAGAGGTAAAGCTGGCTTTCGAGAGGAAAACATGTAAGA AAGTAGCCATAAAGATCATCAGCAAAAGGAAGTTTGCTATTGGTTCAGCAAGAGAGGCAGACCCAGCTCTCAATGTTGAAACAGAAATAGAAATTTTGAA AAAGCTAAATCATCCTTGCATCATCAAGATTAAAAACTTTTTTGATGCAGAAGATTATTATATTGTTTTGGAATTGATGGAAGGGGGAGAGCTGTTTGAC AAAGTGGTGGGGAATAAACGCCTGAAAGAAGCTACCTGCAAGCTCTATTTTTACCAGATGCTCTTGGCTGTGCAGTACCTTCATGAAAACGGTATTATAC ACCGTGACTTAAAGCCAGAGAATGTTTTACTGTCATCTCAAGAAGAGGACTGTCTTATAAAGATTACTGATTTTGGGCACTCCAAGATTTTGGGAGAGAC CTCTCTCATGAGAACCTTATGTGGAACCCCCACCTACTTGGCGCCTGAAGTTCTTGTTTCTGTTGGGACTGCTGGGTATAACCGTGCTGTGGACTGCTGG AGTTTAGGAGTTATTCTTTTTATCTGGACTCAAGTGTCACTGAAGGATCAGATCACCAGTGGAAAATACAACTTCATTCCTGAAGTCTGGGCAGAAGTCT CAGAGAAAGCTCTGGACCTTGTCAAGAAGTTGTTGGTAGTGGATCCAAAGGCACGTTTTACGACAGAAGAAGCCTTAAGACACCCGTGGCTTCAGGATGA AGACATGAAGAGAAAGTTTCAAGATCTTCTGTCTGAGGAAAATGAATCCACAGCTCTACCCCAGGTTCTAGCCCAGCCTTCTACTAGTCGAAAGCGGCCC CGTGAAGGGGAAGCCGAGGGTGCCGAGACCACAAAGCGCCCAGCTGTGTGTGCTGCTGTGTTGTGAACTCCGTGGTTTGAACACGAAAGAAATGTACCTT CTTTCACTCTGTCATCTTTCTTTTCTTTGAGTCTGTTTTTTTATAGTTTGTATTTTAATTATGGGAATAATTGCTTTTTCACAGTCACTGATGTACAATT AAAAACCTGATGGAACCTGGGCTTTGTGCTTCTGCTTGATAATCGGTTCTTTAGTTGAATGGCTTTATTATTTATTTATTTGAGATGGAGTCTCACTCTG TTGCCCAGCCTGAATGAAGTGCAGTGGTGCAGGCTTGGCTCACTGCAACCTCTGCTTCCCAGGTTCAAGCGATTCTTGTGCCTCAGCCTCCTGAGTAGCT GGGATTACAGGTATGCACCACCATGCCTGTTAATTTTTATATATTTTG

CHEK2.u.f.13 (forward primer)
CHEK2.u.r.25 (reverse primer)
Mutually exclusive exon skipping

Frame 0

AFSSASQIRPPLSQARPRRVSRPGPFPETVART SP*G*SSLEWGQVPAALGTVGRRTCPPARGPMGR RSSNSGDNCFPPATQHRSLAVSFWEPLSAKTPT PGAKPRLSPATSGRRPI*PWSLVGKSKESV*PD *CC*LAGESRGRDHPRGTRVLSSALPSKLQLPGG IEAAPDGHICCSSLVRRQGRGF*LAEGGVCICR FSATLLAEAAESVRLQVGSRGRDVSGVGC*GSA VSWQQCLFTAPWQRYPVPRLLLTVPGHIQLLYQH DAKLQPVLSLQLWDTELLRDSVHSGTLFYS*GP RT*GPRT*GAYPCPLGSIMGPSGWICQS*MCE* QLLVWEGQKL*ILL**TTAEKNR*IPNIQQETLS DFQGSGS*KLLHCIHRRSQWQWNLCKYRACRER KTPSFE*QF*NCTVTKQK*SFCLF*SDCR*SVS LS*GIKR*IHHVKNSWKWCLWRGKAGFREENM*E SSHKDHQQKEVCYWFSKRGRPSSQC*NRNRNFE KAKSSLHHQD*KLF*CRRLLYCFGIDGRGRAV* QSGGE*TPERSYLQALFLPDALGCAVPS*KRYYT P*LKARECFTVISRRGLSYKDY*FWALQDFGRD LSHENLMWNPHLLGA*SSCFCWDCWV*PCCGLL EFRSYSFYLDSSVTEGSDHQWKIQLHS*SLGRSL RESSGPCQEVVGSGSKGTFYDRRSLKTPVASG* RHEEKVSRSSV*GK*IHSSTPGSSPAFY*SKAA P*RGSRGCRDHKAPSCVCCCVVNSVV*TRKKCTF FHSVIFLFFESVFL*FVF*LWE*LLFHSH*CTI KNLMEPGLCASA**SVL*LNGFIIYLFEMESHS VAQPE*SAVVQAWLTATSASQVQAILVPQPPE*L GLQVCTTMPVNFYIF

Frame 1

PSLPPARSVLPSRRPDLDVSLARARFPKPSLVP VHKAEVVSSGVRCLQRSALWAEEPVLPPGAPWA AAVPTAGIIASRLRRSIAA*RSPSGNPCRPKPPH PEQSPGSPPPHLAGGLSSRGHSWGKAKRASNQT NVADWLGSRGGGITRGEPGF*VPLSLLNYNSQE ALRRRLTATSAAPHWSGGRGGGFDWLRVEFVSAG LAPLCWLRLRRVCGSRWAHAVVMSRESDVEAQQ SHGSSACSQPHGSVTQSQGSSSQSQGISSSSTS TMPNSSQSSHSSSGTLSSLETVSTQELYSIPEDQ EPEDQEPEEPTPAPWARLWALQDGFANLECVND NYWFGRDKSCEYCFDEPLLKRTDKYRTYSKKHF RIFREVGPKNSYIAYIEDHSGNGTFVNTELVGKG KRRPLNNNSEIALSLSRNKVFVFFDLTVDDQSV YPKALRDEYIMSKTLGSGACGEVKLAFERKTCK KVAIKIISKRKFAIGSAREADPALNVETEIEILK KLNHPCIIKIKNFFDAEDYYIVLELMEGGELFD KVVGNKRLKEATCKLYFYQMLLAVQYLHENGII HRDLKPENVLLSSQEEDCLIKITDFGHSKILGET SLMRTLCGTPTYLAPEVLVSVGTAGYNRAVDCW SLGVILFIWTQVSLKDQITSGKYNFIPEVWAEV SEKALDLVKKLLVVDPKARFTTEEALRHPWLQDE DMKRKFQDLLSEENESTALPQVLAQPSTSRKRP REGEAEGAETTKRPAVCAAVL*TPWFEHERNVP SFTLSSFFSLSLFFYSLYFNYGNNCFFTVTDVQL KT*WNLGFVLLLDNRFFS*MALLFIYLRWSLTL LPSLNEVQWCRLGSLQPLLPRFKRFLCLSLLSS WDYRYAPPCLLIFIYF

Frame 2

LLFRQPDPSSPLAGQTSTCLSPGPVSRNRRSYQ SIRLK*SRVGSGACSARHCGQKNLSSRPGPHGP PQFQQRG*LLPACDAASQLSGLLLGTPVGQNPH TRSKAPALPRHIWPAAYLAVVTRGEKQRERLTRL MLLIGWGVEGAGSPEGNPGSKFRSPF*TTTPRR H*GGA*RPHLLLLIGPAAGEGVLIG*GWSLYLQ V*RHSAG*GCGECAAPGGLTRS*CLGSRMLRLSS LMAAVPVHSPMAALPSPKAPPHSPRAYPAPLPA RCQTPASPLTPALGH*AP*RQCPLRNSILFLRT KNLRTKNLRSLPLPPGLDYGPFRMDLPILNV*MT TTGLGGTKAVNIALMNHC*KEQINTEHTARNTF GFSGKWVLKTLTLHT*KITVAMEPL*IQSL*GK ENAVL*ITILKLHCH*AEIKFLSFLI*L*MISQF ILRH*EMNTSCQKLLEVVPVER*SWLSRGKHVR K*P*RSSAKGSLLLVQQERQTQLSMLKQK*KF* KS*IILASSRLKTFLMQKIIILFWN*WKGESCLT KWWGINA*KKLPASSIFTRCSWLCSTFMKTVLY TVT*SQRMFYCHLKKRTVL*RLLILGTPRFWER PLS*EPYVEPPPTWRLKFLFLLGLLGITVLWTAG V*ELFFLSGLKCH*RIRSPVENTTSFLKSGQKS QRKLWTLSRSCW*WIQRHVLRQKKP*DTRGFRM KT*RESFKIFCLRKMNPQLYPRF*PSLLLVESGP VKGKPRVPRPQSAQLCVLLCCELRGLNTKEMYL LSLCHLSFL*VCFFIVCILIMGIIAFSQSLMYN *KPDGTWALCFCLIIGSLVEWLYYLFI*DGVSLC CPA*MKCSGAGLAHCNLCFPGSSDSCASAS*VA GITGMHHHAC*FLYIL

Sequence CHEK2.cAug05

GCCTTCTCTTCCGCCAGCCAGATCCGTCCTCCCCTCTCGCAGGCCAGACCTCGACGTGTCTCTCGCCCGGGCCCGTTTCCCGAAACCGTCGCTCGTACCA GTCCATAAGGCTGAAGTAGTCTCGAGTGGGGTCAGGTGCCTGCAGCGCTCGGCACTGTGGGCAGAAGAACCTGTCCTCCCGCCCGGGGCCCCATGGGCCG CCGCAGTTCCAACAGCGGGGATAATTGCTTCCCGCCTGCGACGCAGCATCGCAGCTTAGCGGTCTCCTTCTGGGAACCCCTGTCGGCCAAAACCCCCACA CCCGGAGCAAAGCCCCGGCTCTCCCCCGCCACATCTGGCCGGCGGCCTATCTAGCCGTGGTCACTCGTGGGGAAAAGCAAAGAGAGCGTCTAACCAGACT AATGTTGCTGATTGGCTGGGGAGTCGAGGGGGCGGGATCACCCGAGGGGAACCCGGGTTCTAAGTTCCGCTCTCCCTTCTAAACTACAACTCCCAGGAGG CATTGAGGCGGCGCCTGACGGCCACATCTGCTGCTCCTCATTGGTCCGGCGGCAGGGGAGGGGGTTTTGATTGGCTGAGGGTGGAGTTTGTATCTGCAGG TTTAGCGCCACTCTGCTGGCTGAGGCTGCGGAGAGTGTGCGGCTCCAGGTGGGCTCACGCGGTCGTGATGTCTCGGGAGTCGGATGTTGAGGCTCAGCAG TCTCATGGCAGCAGTGCCTGTTCACAGCCCCATGGCAGCGTTACCCAGTCCCAAGGCTCCTCCTCACAGTCCCAGGGCATATCCAGCTCCTCTACCAGCA CGATGCCAAACTCCAGCCAGTCCTCTCACTCCAGCTCTGGGACACTGAGCTCCTTAGAGACAGTGTCCACTCAGGAACTCTATTCTATTCCTGAGGACCA AGAACCTGAGGACCAAGAACCTGAGGAGCCTACCCCTGCCCCCTGGGCTCGATTATGGGCCCTTCAGGATGGATTTGCCAATCTTGAATGTGTGAATGAC AACTACTGGTTTGGGAGGGACAAAAGCTGTGAATATTGCTTTGATGAACCACTGCTGAAAAGAACAGATAAATACCGAACATACAGCAAGAAACACTTTC GGATTTTCAGGGAAGTGGGTCCTAAAAACTCTTACATTGCATACATAGAAGATCACAGTGGCAATGGAACCTTTGTAAATACAGAGCTTGTAGGGAAAGG AAAACGCCGTCCTTTGAATAACAATTCTGAAATTGCACTGTCACTAAGCAGAAATAAAGTTTTTGTCTTTTTTGATCTGACTGTAGATGATCAGTCAGTT TATCCTAAGGCATTAAGAGATGAATACATCATGTCAAAAACTCTTGGAAGTGGTGCCTGTGGAGAGGTAAAGCTGGCTTTCGAGAGGAAAACATGTAAGA AAGTAGCCATAAAGATCATCAGCAAAAGGAAGTTTGCTATTGGTTCAGCAAGAGAGGCAGACCCAGCTCTCAATGTTGAAACAGAAATAGAAATTTTGAA AAAGCTAAATCATCCTTGCATCATCAAGATTAAAAACTTTTTTGATGCAGAAGATTATTATATTGTTTTGGAATTGATGGAAGGGGGAGAGCTGTTTGAC AAAGTGGTGGGGAATAAACGCCTGAAAGAAGCTACCTGCAAGCTCTATTTTTACCAGATGCTCTTGGCTGTGCAGTACCTTCATGAAAACGGTATTATAC ACCGTGACTTAAAGCCAGAGAATGTTTTACTGTCATCTCAAGAAGAGGACTGTCTTATAAAGATTACTGATTTTGGGCACTCCAAGATTTTGGGAGAGAC CTCTCTCATGAGAACCTTATGTGGAACCCCCACCTACTTGGCGCCTGAAGTTCTTGTTTCTGTTGGGACTGCTGGGTATAACCGTGCTGTGGACTGCTGG AGTTTAGGAGTTATTCTTTTTATCTGCCTTAGTGGGTATCCACCTTTCTCTGAGCATAGGACTCAAGTGTCACTGAAGGATCAGATCACCAGTGGAAAAT ACAACTTCATTCCTGAAGTCTGGGCAGAAGTCTCAGAGAAAGCTCTGGACCTTGTCAAGAAGTTGTTGGTAGTGGATCCAAAGGCACGTTTTACGACAGA AGAAGCCTTAAGACACCCGTGGCTTCAGGATGAAGACATGAAGAGAAAGTTTCAAGATCTTCTGTCTGAGGAAAATGAATCCACAGCTCTACCCCAGGTT CTAGCCCAGCCTTCTACTAGTCGAAAGCGGCCCCGTGAAGGGGAAGCCGAGGGTGCCGAGACCACAAAGCGCCCAGCTGTGTGTGCTGCTGTGTTGTGAA CTCCGTGGTTTGAACACGAAAGAAATGTACCTTCTTTCACTCTGTCATCTTTCTTTTCTTTGAGTCTGTTTTTTTATAGTTTGTATTTTAATTATGGGAA TAATTGCTTTTTCACAGTCACTGATGTACAATTAAAAACCTGATGGAACCTGGGCTTTGTGCTTCTGCTTGATAATCGGTTCTTTAGTTGAATGGCTTTA TTATTTATTTATTTGAGATGGAGTCTCACTCTGTTGCCCAGCCTGAATGAAGTGCAGTGGTGCAGGCTTGGCTCACTGCAACCTCTGCTTCCCAGGTTCA AGCGATTCTTGTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGTATGCACCACCATGCCTGTTAATTTTTATATATTTTG

CHEK2.u.f.13 (forward primer)
CHEK2.u.r.25 (reverse primer)
Mutually exclusive exon skipping

Frame 0

AFSSASQIRPPLSQARPRRVSRPGPFPETVART SP*G*SSLEWGQVPAALGTVGRRTCPPARGPMGR RSSNSGDNCFPPATQHRSLAVSFWEPLSAKTPT PGAKPRLSPATSGRRPI*PWSLVGKSKESV*PD *CC*LAGESRGRDHPRGTRVLSSALPSKLQLPGG IEAAPDGHICCSSLVRRQGRGF*LAEGGVCICR FSATLLAEAAESVRLQVGSRGRDVSGVGC*GSA VSWQQCLFTAPWQRYPVPRLLLTVPGHIQLLYQH DAKLQPVLSLQLWDTELLRDSVHSGTLFYS*GP RT*GPRT*GAYPCPLGSIMGPSGWICQS*MCE* QLLVWEGQKL*ILL**TTAEKNR*IPNIQQETLS DFQGSGS*KLLHCIHRRSQWQWNLCKYRACRER KTPSFE*QF*NCTVTKQK*SFCLF*SDCR*SVS LS*GIKR*IHHVKNSWKWCLWRGKAGFREENM*E SSHKDHQQKEVCYWFSKRGRPSSQC*NRNRNFE KAKSSLHHQD*KLF*CRRLLYCFGIDGRGRAV* QSGGE*TPERSYLQALFLPDALGCAVPS*KRYYT P*LKARECFTVISRRGLSYKDY*FWALQDFGRD LSHENLMWNPHLLGA*SSCFCWDCWV*PCCGLL EFRSYSFYLP*WVSTFL*A*DSSVTEGSDHQWKI QLHS*SLGRSLRESSGPCQEVVGSGSKGTFYDR RSLKTPVASG*RHEEKVSRSSV*GK*IHSSTPG SSPAFY*SKAAP*RGSRGCRDHKAPSCVCCCVVN SVV*TRKKCTFFHSVIFLFFESVFL*FVF*LWE *LLFHSH*CTIKNLMEPGLCASA**SVL*LNGF IIYLFEMESHSVAQPE*SAVVQAWLTATSASQVQ AILVPQPPE*LGLQVCTTMPVNFYIF

Frame 1

PSLPPARSVLPSRRPDLDVSLARARFPKPSLVP VHKAEVVSSGVRCLQRSALWAEEPVLPPGAPWA AAVPTAGIIASRLRRSIAA*RSPSGNPCRPKPPH PEQSPGSPPPHLAGGLSSRGHSWGKAKRASNQT NVADWLGSRGGGITRGEPGF*VPLSLLNYNSQE ALRRRLTATSAAPHWSGGRGGGFDWLRVEFVSAG LAPLCWLRLRRVCGSRWAHAVVMSRESDVEAQQ SHGSSACSQPHGSVTQSQGSSSQSQGISSSSTS TMPNSSQSSHSSSGTLSSLETVSTQELYSIPEDQ EPEDQEPEEPTPAPWARLWALQDGFANLECVND NYWFGRDKSCEYCFDEPLLKRTDKYRTYSKKHF RIFREVGPKNSYIAYIEDHSGNGTFVNTELVGKG KRRPLNNNSEIALSLSRNKVFVFFDLTVDDQSV YPKALRDEYIMSKTLGSGACGEVKLAFERKTCK KVAIKIISKRKFAIGSAREADPALNVETEIEILK KLNHPCIIKIKNFFDAEDYYIVLELMEGGELFD KVVGNKRLKEATCKLYFYQMLLAVQYLHENGII HRDLKPENVLLSSQEEDCLIKITDFGHSKILGET SLMRTLCGTPTYLAPEVLVSVGTAGYNRAVDCW SLGVILFICLSGYPPFSEHRTQVSLKDQITSGK YNFIPEVWAEVSEKALDLVKKLLVVDPKARFTTE EALRHPWLQDEDMKRKFQDLLSEENESTALPQV LAQPSTSRKRPREGEAEGAETTKRPAVCAAVL* TPWFEHERNVPSFTLSSFFSLSLFFYSLYFNYGN NCFFTVTDVQLKT*WNLGFVLLLDNRFFS*MAL LFIYLRWSLTLLPSLNEVQWCRLGSLQPLLPRF KRFLCLSLLSSWDYRYAPPCLLIFIYF

Frame 2

LLFRQPDPSSPLAGQTSTCLSPGPVSRNRRSYQ SIRLK*SRVGSGACSARHCGQKNLSSRPGPHGP PQFQQRG*LLPACDAASQLSGLLLGTPVGQNPH TRSKAPALPRHIWPAAYLAVVTRGEKQRERLTRL MLLIGWGVEGAGSPEGNPGSKFRSPF*TTTPRR H*GGA*RPHLLLLIGPAAGEGVLIG*GWSLYLQ V*RHSAG*GCGECAAPGGLTRS*CLGSRMLRLSS LMAAVPVHSPMAALPSPKAPPHSPRAYPAPLPA RCQTPASPLTPALGH*AP*RQCPLRNSILFLRT KNLRTKNLRSLPLPPGLDYGPFRMDLPILNV*MT TTGLGGTKAVNIALMNHC*KEQINTEHTARNTF GFSGKWVLKTLTLHT*KITVAMEPL*IQSL*GK ENAVL*ITILKLHCH*AEIKFLSFLI*L*MISQF ILRH*EMNTSCQKLLEVVPVER*SWLSRGKHVR K*P*RSSAKGSLLLVQQERQTQLSMLKQK*KF* KS*IILASSRLKTFLMQKIIILFWN*WKGESCLT KWWGINA*KKLPASSIFTRCSWLCSTFMKTVLY TVT*SQRMFYCHLKKRTVL*RLLILGTPRFWER PLS*EPYVEPPPTWRLKFLFLLGLLGITVLWTAG V*ELFFLSALVGIHLSLSIGLKCH*RIRSPVEN TTSFLKSGQKSQRKLWTLSRSCW*WIQRHVLRQ KKP*DTRGFRMKT*RESFKIFCLRKMNPQLYPRF *PSLLLVESGPVKGKPRVPRPQSAQLCVLLCCE LRGLNTKEMYLLSLCHLSFL*VCFFIVCILIMG IIAFSQSLMYN*KPDGTWALCFCLIIGSLVEWLY YLFI*DGVSLCCPA*MKCSGAGLAHCNLCFPGS SDSCASAS*VAGITGMHHHAC*FLYIL

Sequence CHEK2.bAug05

GCCTTCTCTTCCGCCAGCCAGATCCGTCCTCCCCTCTCGCAGGCCAGACCTCGACGTGTCTCTCGCCCGGGCCCGTTTCCCGAAACCGTCGCTCGTACCA GTCCATAAGGCTGAAGTAGTCTCGAGTGGGGTCAGGTGCCTGCAGCGCTCGGCACTGTGGGCAGAAGAACCTGTCCTCCCGCCCGGGGCCCCATGGGCCG CCGCAGTTCCAACAGCGGGGATAATTGCTTCCCGCCTGCGACGCAGCATCGCAGCTTAGCGGTCTCCTTCTGGGAACCCCTGTCGGCCAAAACCCCCACA CCCGGAGCAAAGCCCCGGCTCTCCCCCGCCACATCTGGCCGGCGGCCTATCTAGCCGTGGTCACTCGTGGGGAAAAGCAAAGAGAGCGTCTAACCAGACT AATGTTGCTGATTGGCTGGGGAGTCGAGGGGGCGGGATCACCCGAGGGGAACCCGGGTTCTAAGTTCCGCTCTCCCTTCTAAACTACAACTCCCAGGAGG CATTGAGGCGGCGCCTGACGGCCACATCTGCTGCTCCTCATTGGTCCGGCGGCAGGGGAGGGGGTTTTGATTGGCTGAGGGTGGAGTTTGTATCTGCAGG TTTAGCGCCACTCTGCTGGCTGAGGCTGCGGAGAGTGTGCGGCTCCAGGTGGGCTCACGCGATACTGGATTTCACCATGGTGCCCAGGCTGGTCTTGAAT TCCTGGGCTCAAGCAATCTACCTGCGTTGGCCTCCCAAAGTGCTGGGATTACAGACATGAACCACCACACCTGGCAGAACCTTTGTATCTTGAAAGCTGT GTAAATACAATCAGTGTCGTGATGTCTCGGGAGTCGGATGTTGAGGCTCAGCAGTCTCATGGCAGCAGTGCCTGTTCACAGCCCCATGGCAGCGTTACCC AGTCCCAAGGCTCCTCCTCACAGTCCCAGGGCATATCCAGCTCCTCTACCAGCACGATGCCAAACTCCAGCCAGTCCTCTCACTCCAGCTCTGGGACACT GAGCTCCTTAGAGACAGTGTCCACTCAGGAACTCTATTCTATTCCTGAGGACCAAGAACCTGAGGACCAAGAACCTGAGGAGCCTACCCCTGCCCCCTGG GCTCGATTATGGGCCCTTCAGGATGGATTTGCCAATCTTGAATGTGTGAATGACAACTACTGGTTTGGGAGGGACAAAAGCTGTGAATATTGCTTTGATG AACCACTGCTGAAAAGAACAGATAAATACCGAACATACAGCAAGAAACACTTTCGGATTTTCAGGGAAGTGGGTCCTAAAAACTCTTACATTGCATACAT AGAAGATCACAGTGGCAATGGAACCTTTGTAAATACAGAGCTTGTAGGGAAAGGAAAACGCCGTCCTTTGAATAACAATTCTGAAATTGCACTGTCACTA AGCAGAAATAAAGTTTTTGTCTTTTTTGATCTGACTGTAGATGATCAGTCAGTTTATCCTAAGGCATTAAGAGATGAATACATCATGTCAAAAACTCTTG GAAGTGGTGCCTGTGGAGAGGTAAAGCTGGCTTTCGAGAGGAAAACATGTAAGAAAGTAGCCATAAAGATCATCAGCAAAAGGAAGTTTGCTATTGGTTC AGCAAGAGAGGCAGACCCAGCTCTCAATGTTGAAACAGAAATAGAAATTTTGAAAAAGCTAAATCATCCTTGCATCATCAAGATTAAAAACTTTTTTGAT GCAGAAGATTATTATATTGTTTTGGAATTGATGGAAGGGGGAGAGCTGTTTGACAAAGTGGTGGGGAATAAACGCCTGAAAGAAGCTACCTGCAAGCTCT ATTTTTACCAGATGCTCTTGGCTGTGCAGTACCTTCATGAAAACGGTATTATACACCGTGACTTAAAGCCAGAGAATGTTTTACTGTCATCTCAAGAAGA GGACTGTCTTATAAAGATTACTGATTTTGGGCACTCCAAGATTTTGGGAGAGACCTCTCTCATGAGAACCTTATGTGGAACCCCCACCTACTTGGCGCCT GAAGTTCTTGTTTCTGTTGGGACTGCTGGGTATAACCGTGCTGTGGACTGCTGGAGTTTAGGAGTTATTCTTTTTATCTGCCTTAGTGGGTATCCACCTT TCTCTGAGCATAGGACTCAAGTGTCACTGAAGGATCAGATCACCAGTGGAAAATACAACTTCATTCCTGAAGTCTGGGCAGAAGTCTCAGAGAAAGCTCT GGACCTTGTCAAGAAGTTGTTGGTAGTGGATCCAAAGGCACGTTTTACGACAGAAGAAGCCTTAAGACACCCGTGGCTTCAGGATGAAGACATGAAGAGA AAGTTTCAAGATCTTCTGTCTGAGGAAAATGAATCCACAGCTCTACCCCAGGTTCTAGCCCAGCCTTCTACTAGTCGAAAGCGGCCCCGTGAAGGGGAAG CCGAGGGTGCCGAGACCACAAAGCGCCCAGCTGTGTGTGCTGCTGTGTTGTGAACTCCGTGGTTTGAACACGAAAGAAATGTACCTTCTTTCACTCTGTC ATCTTTCTTTTCTTTGAGTCTGTTTTTTTATAGTTTGTATTTTAATTATGGGAATAATTGCTTTTTCACAGTCACTGATGTACAATTAAAAACCTGATGG AACCTGGGCTTTGTGCTTCTGCTTGATAATCGGTTCTTTAGTTGAATGGCTTTATTATTTATTTATTTGAGATGGAGTCTCACTCTGTTGCCCAGCCTGA ATGAAGTGCAGTGGTGCAGGCTTGGCTCACTGCAACCTCTGCTTCCCAGGTTCAAGCGATTCTTGTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGTA TGCACCACCATGCCTGTTAATTTTTATATATTTTG

CHEK2.u.f.13 (forward primer)
CHEK2.u.r.25 (reverse primer)
Mutually exclusive exon skipping

Frame 0

AFSSASQIRPPLSQARPRRVSRPGPFPETVART SP*G*SSLEWGQVPAALGTVGRRTCPPARGPMGR RSSNSGDNCFPPATQHRSLAVSFWEPLSAKTPT PGAKPRLSPATSGRRPI*PWSLVGKSKESV*PD *CC*LAGESRGRDHPRGTRVLSSALPSKLQLPGG IEAAPDGHICCSSLVRRQGRGF*LAEGGVCICR FSATLLAEAAESVRLQVGSRDTGFHHGAQAGLE FLGSSNLPALASQSAGITDMNHHTWQNLCILKAV *IQSVS*CLGSRMLRLSSLMAAVPVHSPMAALP SPKAPPHSPRAYPAPLPARCQTPASPLTPALGH *AP*RQCPLRNSILFLRTKNLRTKNLRSLPLPPG LDYGPFRMDLPILNV*MTTTGLGGTKAVNIALM NHC*KEQINTEHTARNTFGFSGKWVLKTLTLHT *KITVAMEPL*IQSL*GKENAVL*ITILKLHCH* AEIKFLSFLI*L*MISQFILRH*EMNTSCQKLL EVVPVER*SWLSRGKHVRK*P*RSSAKGSLLLV QQERQTQLSMLKQK*KF*KS*IILASSRLKTFLM QKIIILFWN*WKGESCLTKWWGINA*KKLPASS IFTRCSWLCSTFMKTVLYTVT*SQRMFYCHLKK RTVL*RLLILGTPRFWERPLS*EPYVEPPPTWRL KFLFLLGLLGITVLWTAGV*ELFFLSALVGIHL SLSIGLKCH*RIRSPVENTTSFLKSGQKSQRKL WTLSRSCW*WIQRHVLRQKKP*DTRGFRMKT*RE SFKIFCLRKMNPQLYPRF*PSLLLVESGPVKGK PRVPRPQSAQLCVLLCCELRGLNTKEMYLLSLC HLSFL*VCFFIVCILIMGIIAFSQSLMYN*KPDG TWALCFCLIIGSLVEWLYYLFI*DGVSLCCPA* MKCSGAGLAHCNLCFPGSSDSCASAS*VAGITG MHHHAC*FLYIL

Frame 1

PSLPPARSVLPSRRPDLDVSLARARFPKPSLVP VHKAEVVSSGVRCLQRSALWAEEPVLPPGAPWA AAVPTAGIIASRLRRSIAA*RSPSGNPCRPKPPH PEQSPGSPPPHLAGGLSSRGHSWGKAKRASNQT NVADWLGSRGGGITRGEPGF*VPLSLLNYNSQE ALRRRLTATSAAPHWSGGRGGGFDWLRVEFVSAG LAPLCWLRLRRVCGSRWAHAILDFTMVPRLVLN SWAQAIYLRWPPKVLGLQT*TTTPGRTFVS*KL CKYNQCRDVSGVGC*GSAVSWQQCLFTAPWQRYP VPRLLLTVPGHIQLLYQHDAKLQPVLSLQLWDT ELLRDSVHSGTLFYS*GPRT*GPRT*GAYPCPL GSIMGPSGWICQS*MCE*QLLVWEGQKL*ILL** TTAEKNR*IPNIQQETLSDFQGSGS*KLLHCIH RRSQWQWNLCKYRACRERKTPSFE*QF*NCTVT KQK*SFCLF*SDCR*SVSLS*GIKR*IHHVKNSW KWCLWRGKAGFREENM*ESSHKDHQQKEVCYWF SKRGRPSSQC*NRNRNFEKAKSSLHHQD*KLF* CRRLLYCFGIDGRGRAV*QSGGE*TPERSYLQAL FLPDALGCAVPS*KRYYTP*LKARECFTVISRR GLSYKDY*FWALQDFGRDLSHENLMWNPHLLGA *SSCFCWDCWV*PCCGLLEFRSYSFYLP*WVSTF L*A*DSSVTEGSDHQWKIQLHS*SLGRSLRESS GPCQEVVGSGSKGTFYDRRSLKTPVASG*RHEE KVSRSSV*GK*IHSSTPGSSPAFY*SKAAP*RGS RGCRDHKAPSCVCCCVVNSVV*TRKKCTFFHSV IFLFFESVFL*FVF*LWE*LLFHSH*CTIKNLM EPGLCASA**SVL*LNGFIIYLFEMESHSVAQPE *SAVVQAWLTATSASQVQAILVPQPPE*LGLQV CTTMPVNFYIF

Frame 2

LLFRQPDPSSPLAGQTSTCLSPGPVSRNRRSYQ SIRLK*SRVGSGACSARHCGQKNLSSRPGPHGP PQFQQRG*LLPACDAASQLSGLLLGTPVGQNPH TRSKAPALPRHIWPAAYLAVVTRGEKQRERLTRL MLLIGWGVEGAGSPEGNPGSKFRSPF*TTTPRR H*GGA*RPHLLLLIGPAAGEGVLIG*GWSLYLQ V*RHSAG*GCGECAAPGGLTRYWISPWCPGWS*I PGLKQSTCVGLPKCWDYRHEPPHLAEPLYLESC VNTISVVMSRESDVEAQQSHGSSACSQPHGSVT QSQGSSSQSQGISSSSTSTMPNSSQSSHSSSGTL SSLETVSTQELYSIPEDQEPEDQEPEEPTPAPW ARLWALQDGFANLECVNDNYWFGRDKSCEYCFD EPLLKRTDKYRTYSKKHFRIFREVGPKNSYIAYI EDHSGNGTFVNTELVGKGKRRPLNNNSEIALSL SRNKVFVFFDLTVDDQSVYPKALRDEYIMSKTL GSGACGEVKLAFERKTCKKVAIKIISKRKFAIGS AREADPALNVETEIEILKKLNHPCIIKIKNFFD AEDYYIVLELMEGGELFDKVVGNKRLKEATCKL YFYQMLLAVQYLHENGIIHRDLKPENVLLSSQEE DCLIKITDFGHSKILGETSLMRTLCGTPTYLAP EVLVSVGTAGYNRAVDCWSLGVILFICLSGYPP FSEHRTQVSLKDQITSGKYNFIPEVWAEVSEKAL DLVKKLLVVDPKARFTTEEALRHPWLQDEDMKR KFQDLLSEENESTALPQVLAQPSTSRKRPREGE AEGAETTKRPAVCAAVL*TPWFEHERNVPSFTLS SFFSLSLFFYSLYFNYGNNCFFTVTDVQLKT*W NLGFVLLLDNRFFS*MALLFIYLRWSLTLLPSL NEVQWCRLGSLQPLLPRFKRFLCLSLLSSWDYRY APPCLLIFIYF