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Sequences

Sequence WISP1.bAug05

ATATCTGGTGCTCCTGATGGGCCGGCCAGTCTGGGCCCAGCTCCCCCGAGAGGTGGTCGGATCCTCTGGGCTGCTCGGTCGATGCCTGTGCCACTGACGT CCAGGCATGAGGTGGTTCCTGCCCTGGACGCTGGCAGCAGTGACAGCAGCAGCCGCCAGCACCGTCCTGGCCACGGCCCTCTCTCCAGCCCCTACGACCA TGGACTTTACCCCAGCTCCACTGGAGGACACCTCCTCACGCCCCCAATTCTGCAAGTGGCCATGTGAGTGCCCGCCATCCCCACCCCGCTGCCCGCTGGG GGTCAGCCTCATCACAGATGGCTGTGAGTGCTGTAAGATGTGCGCTCAGCAGCTTGGGGACAACTGCACGGAGGCTGCCATCTGTGACCCCCACCGGGGC CTCTACTGTGACTACAGCGGGGACCGCCCGAGGTACGCAATAGGAGTGTGTGCACATGCTGTGGGTGAGGTGGAGGCATGGCACAGGAACTGCATAGCCT ACACAAGCCCCTGGAGCCCTTGCTCCACCAGCTGCGGCCTGGGGGTCTCCACTCGGATCTCCAATGTTAACGCCCAGTGCTGGCCTGAGCAAGAGAGCCG CCTCTGCAACTTGCGGCCATGCGATGTGGACATCCATACACTCATTAAGGCAGGGAAGAAGTGTCTGGCTGTGTACCAGCCAGAGGCATCCATGAACTTC ACACTTGCGGGCTGCATCAGCACACGCTCCTATCAACCCAAGTACTGTGGAGTTTGCATGGACAATAGGTGCTGCATCCCCTACAAGTCTAAGACTATCG ACGTGTCCTTCCAGTGTCCTGATGGGCTTGGCTTCTCCCGCCAGGTCCTATGGATTAATGCCTGCTTCTGTAACCTGAGCTGTAGGAATCCCAATGACAT CTTTGCTGACTTGGAATCCTACCCTGACTTCTCAGAAATTGCCAACTAGGCAGGCACAAATCTTGGGTCTTGGGGACTAACCCAATGCCTGTGAAGCAGT CAGCCCTTATGGCCAATAACTTTTCACCAATGAGCCTTAGTTACCCTGATCTGGACCCTTGGCCTCCATTTCTGTCTCTAACCATTCAAATGACGCCTGA TGGTGCTGCTCAGGCCCATGCTATGAGTTTTCTCCTTGATATCATTCAGCATCTACTCTAAAGAAAAATGCCTGTCTCTAGCTGTTCTGGACTACACCCA AGCCTGATCCAGCCTTTCCAAGTCACTAGAAGTCCTGCTGGATCTTGCCTAAATCCCAAGAAATGGAATCAGGTAGACTTTTAATATCACTAATTTCTTC TTTAGATGCCAAACCACAAGACTCTTTGGGTCCATTCAGATGAATAGATGGAATTTGGAACAATAGAATAATCTATTATTTGGAGCCTGCCAAGAGGTAC TGTAATGGGTAATTCTGACGTCAGCGCACCAAAACTATCCTGATTCCAAATATGTATGCACCTCAAGGTCATCAAACATTTGCCAAGTGAGTTGAATAGT TGCTTAATTTTGATTTTTAATGGAAAGTTGTATCCATTAACCTGGGCATTGTTGAGGTTAAGTTTCTCTTCACCCCTACACTGTGAAGGGTACAGATTAG GTTTGTCCCAGTCAGAAATAAAATTTGATAAACATTCCTGTTGATGGGAAAAGCCCCCAGTTAATACTCCAGAGACAGGGAAAGGTCAGCCCGTTTCAGA AGGACCAATTGACTCTCACACTGAATCAGCTGCTGACTGGCAGGGCTTTGGGCAGTTGGCCAGGCTCTTCCTTGAATCTTCTCCCTTGTCCTGCTTGGGG TTCATAGGAATTGGTAAGGCCTCTGGACTGGCCTGTCTGGCCCCTGAGAGTGGTGCCCTGGAACACTCCTCTACTCTTACAGAGCCTTGAGAGACCCAGC TGCAGACCATGCCAGACCCACTGAAATGACCAAGACAGGTTCAGGTAGGGGTGTGGGTCAAACCAAGAAGTGGGTGCCCTTGGTAGCAGCCTGGGGTGAC CTCTAGAGCTGGAGGCTGTGGGACTCCAGGGGCCCCCGTGTTCAGGACACATCTATTGCAGAGACTCATTTCACAGCCTTTCGTTCTGCTGACCAAATGG CCAGTTTTCTGGTAGGAAGATGGAGGTTTACCGGTTGTTTAGAAACAGAAATAGACTTAATAAAGGTTTAAAGCTGAAGAGGTTGAAGCTAAAAGGAAAA GGTTGTTGTTAATGAATATCAGGCTATTATTTATTGTATTAGGAAAATATAATATTTACTGTTAGAATTCTTTTATTTAGGGCCTTTTCTGTGCCAGACA TTGCTCTCAGTGCTTTGCATGTATTAGCTCACTGAATCTTCACGACAATGTTGAGAAGTTCCCATTATTATTTCTGTTCTTACAAATGTGAAACGGAAGC TCATAGAGGTGAGAAAACTCAACCAGAGTCACCCAGTTGGTGACTGGGAAAGTTAGGATTCAGATCGAAATTGGACTGTCTTTATAACCCATATTTTCCC CCTGTTTTTAGAGCTTCCAAATGTGTCAGAATAGGAAAACATTGCAATAAATGGCTTGATTTTTTAATGTCATTTTTCCCTCTTATAGTCTTTCTAGCTC CTTTTCAAAAGACGAGAATATCTGATTTTCTGATAATTTAGGTGCTTAAGCATCCAAAATACATGGGACACACAAAAATCCAGGAATCCCCTGTAGCTTA TTCCCTCTTTCCCATCGGAACCAGCTCTCATCACACATTTAAAAGATGATTCTGTTTACCCAATGCTGCATATTGAATGTTGTGTAGTTATTCACAGGGA ATTCTGTGCAGTGTGCAGAGAGATTCCTAAACGGGAAAAGGACTGGGAATACATCCTCCTTACTGTGACCTCCCCAAAACCTAGTCCAGTGCAAGGTATA CAGTGGTGCTCATTAAATACTTGATGAATACAGGAAGCTGTGCATGTGTTCCTACTTTTATTCGAAGCTCTCTTCTTCCAAAGCTACATGAAAATAGAAT TTTAACAGTCAAAATTTTATATTAAGTGCCTTAGCAAAAGAGACATTTAATATTTCAAAGAAATGCATATGTATGTATACATATATTTGTGTATGCGTAT GCAAGAATTCTTGTATAAAGAGAATTCACTCCATGAATGATCTCTTCTGTAAGTCAGTGTGAATCATGTTAGATTTTCTGAGAGTGAAAACACCTGCCAT CTACAAATTACAAGGCTGGATAACAGCTCACTCCATTTGAAATTCAGTGGAAACCCAAGAGCTAGGTTCTTACTGAATTTGCATCTCAATTTGGGAAACT GAACTTAGCTTTCAAAGATCATAGGAAGTCTGGTTGGAGAAACTAGGGATTATTCTGGCAATGGGTGCAGGAAGGTGGTCAGAATAACCCAGTCGCCATT GGTTTTGAGAAACGGAACTATCTTATGCAGAGCCCGGAGGGCAAGTCTCAGACCCATGGGTTGAAGCCATGGAGAAGGAAATTTGGATCCAATGTAATGA AGCGCTTTCTAAGTCAGAATTTCCCTGCAATGGTGTGGCCTGATTCAATAAAAATTAAGAATAATAAATATAATGGAAAAAAATCTCCACTGATTGAGTG TTTACTTGGTGCCAAGCACTATGCTAAGTTGTTCATTATTTTATTTAATTGTTACAGCAATTTTGAGTATGCATCTTTCACTATTTTATAAGTGGAAAAG AGAAGTGCCCCCAAAAAGTTAGAGCTCAAACAGCAGCTTATTCTACCAGCCCCTGCTCTTGCGGAGGCCTCTGGAAAAGACCTGAATGACACCTATTGGA GAATTACATCTACAAGGGGCTTCAAACAGACCAAATAGATCATCACCTCTGTGGTCCCTTGTTAACTATATGTTCTGAGACAAAGGAAAGCTACCCTAAG GGTTAGTTAACCTTTGCTGAGGAAATTTACATTCATACTTAGAGTGAATTACTCAGGTGTGCTTAGGTGTGCAAAAGGGAAGGAGACCTGAATTCACCAA GTTAAATCTTGCTAAACCTTATCATAAGCATTTTTTGAGCGCTTAGCATACACCAAGCCTTGTGGAAGGTGCTTTCCTGCCATATCTCATTTAATCCTCA CAGCAAACCTATAGAATATGGCATTATCATCTGAGTCTCACAGAAGTTTAGTCGTGTACTCAAGGTCTTACCAGCTAGTGAACAGCAGACCAAGACTGGA AACCCAGGATAGTCTGATACCTGAGCCATCTCTTCTTGTGCTACGCCTAGTTATTCTGTCCCCCAAATCAAAAGGCATGACCTTTATAAGAGGCGCTTTA CTGACAATAGCTGCAATTTTAACTTTGAAAATGATTCAGAATTATCAAAGATAGTAGATTCGAATGACATGATTGTCTATAATCTCGCTAGCCTTGTACT GTGTGTGCATAGCAATTACAGGGAAGTAATCTAGCTCCTGACTATTATGTTGAACTATGTCGCTGCTTTTTACAAACTTGTCTTGATCCAAAGCAGTCAC AATGATAACCCTGCATATCTGGGAATCATAAGTCAACTATGTATCCCTGTGTGTGTATATATATGTATGTATGTATCTATTTTCAAACTGTGATTTAATA TTTAAATATTCCTACTGCCATTTTTGTGACTGAAAAACTACACATGAGGAAACGTCTTAGAATTTTCCAATAGAGGAAAAATAACACTTGGGCAATCTGT CATGTTTCACAACAGTTCTCATTTTTCTCATGATTTGTGTAGCGTGGAATGTGTTTGCTCAATGTGAAGGGTTTTCATTGCTCAATTTCTCTGTGTAAGT CTTTTCCTTAAGGTAATAAACCATCAGCAAAGTCACATACTGGAGTTGGTGGCTTTTCTTGTACAGGCAGTTGTTATGAGACAATGATGGAGCATTGAGC ATGTTCAATAAATGTGCAGATGGTGGAAA

WISP1.u.f.4 (forward primer)
WISP1.u.r.4 (reverse primer)
Single exon skipping

Frame 0

ISGAPDGPASLGPAPPRGGRILWAARSMPVPLT SRHEVVPALDAGSSDSSSRQHRPGHGPLSSPYDH GLYPSSTGGHLLTPPILQVAM*VPAIPTPLPAG GQPHHRWL*VL*DVRSAAWGQLHGGCHL*PPPG PLL*LQRGPPEVRNRSVCTCCG*GGGMAQELHSL HKPLEPLLHQLRPGGLHSDLQC*RPVLA*AREP PLQLAAMRCGHPYTH*GREEVSGCVPARGIHEL HTCGLHQHTLLSTQVLWSLHGQ*VLHPLQV*DYR RVLPVS*WAWLLPPGPMD*CLLL*PEL*ESQ*H LC*LGILP*LLRNCQLGRHKSWVLGTNPMPVKQ SALMANNFSPMSLSYPDLDPWPPFLSLTIQMTPD GAAQAHAMSFLLDIIQHLL*RKMPVSSCSGLHP SLIQPFQVTRSPAGSCLNPKKWNQVDF*YH*FL L*MPNHKTLWVHSDE*MEFGTIE*SIIWSLPRGT VMGNSDVSAPKLS*FQICMHLKVIKHLPSELNS CLILIFNGKLYPLTWALLRLSFSSPLHCEGYRL GLSQSEIKFDKHSC*WEKPPVNTPETGKGQPVSE GPIDSHTESAADWQGFGQLARLFLESSPLSCLG FIGIGKASGLACLAPESGALEHSSTLTEP*ETQ LQTMPDPLK*PRQVQVGVWVKPRSGCPW*QPGVT SRAGGCGTPGAPVFRTHLLQRLISQPFVLLTKW PVFW*EDGGLPVV*KQK*T**RFKAEEVEAKRK RLLLMNIRLLFIVLGKYNIYC*NSFI*GLFCARH CSQCFACISSLNLHDNVEKFPLLFLFLQM*NGS S*R*ENSTRVTQLVTGKVRIQIEIGLSL*PIFS PCF*SFQMCQNRKTLQ*MA*FFNVIFPSYSLSSS FSKDENI*FSDNLGA*ASKIHGTHKNPGIPCSL FPLSHRNQLSSHI*KMILFTQCCILNVV*LFTG NSVQCAERFLNGKRTGNTSSLL*PPQNLVQCKVY SGAH*ILDEYRKLCMCSYFYSKLSSSKAT*K*N FNSQNFILSALAKETFNISKKCICMYTYICVCV CKNSCIKRIHSMNDLFCKSV*IMLDFLRVKTPAI YKLQGWITAHSI*NSVETQELGSY*ICISIWET ELSFQRS*EVWLEKLGIILAMGAGRWSE*PSRH WF*ETELSYAEPGGQVSDPWVEAMEKEIWIQCNE ALSKSEFPCNGVA*FNKN*E**I*WKKISTD*V FTWCQALC*VVHYFI*LLQQF*VCIFHYFISGK EKCPQKVRAQTAAYSTSPCSCGGLWKRPE*HLLE NYIYKGLQTDQIDHHLCGPLLTICSETKESYPK G*LTFAEEIYIHT*SELLRCA*VCKREGDLNSP S*ILLNLIISIF*ALSIHQALWKVLSCHISFNPH SKPIEYGIII*VSQKFSRVLKVLPASEQQTKTG NPG*SDT*AISSCATPSYSVPQIKRHDLYKRRF TDNSCNFNFENDSELSKIVDSNDMIVYNLASLVL CVHSNYREVI*LLTIMLNYVAAFYKLVLIQSSH NDNPAYLGIISQLCIPVCVYICMYVSIFKL*FN I*IFLLPFL*LKNYT*GNVLEFSNRGKITLGQSV MFHNSSHFSHDLCSVECVCSM*RVFIAQFLCVS LFLKVINHQQSHILELVAFLVQAVVMRQ*WSIE HVQ*MCRWWK

Frame 1

YLVLLMGRPVWAQLPREVVGSSGLLGRCLCH*R PGMRWFLPWTLAAVTAAAASTVLATALSPAPTT MDFTPAPLEDTSSRPQFCKWPCECPPSPPRCPLG VSLITDGCECCKMCAQQLGDNCTEAAICDPHRG LYCDYSGDRPRYAIGVCAHAVGEVEAWHRNCIA YTSPWSPCSTSCGLGVSTRISNVNAQCWPEQESR LCNLRPCDVDIHTLIKAGKKCLAVYQPEASMNF TLAGCISTRSYQPKYCGVCMDNRCCIPYKSKTI DVSFQCPDGLGFSRQVLWINACFCNLSCRNPNDI FADLESYPDFSEIAN*AGTNLGSWGLTQCL*SS QPLWPITFHQ*ALVTLIWTLGLHFCL*PFK*RL MVLLRPML*VFSLISFSIYSKEKCLSLAVLDYTQ A*SSLSKSLEVLLDLA*IPRNGIR*TFNITNFF FRCQTTRLFGSIQMNRWNLEQ*NNLLFGACQEV L*WVILTSAHQNYPDSKYVCTSRSSNICQVS*IV A*F*FLMESCIH*PGHC*G*VSLHPYTVKGTD* VCPSQK*NLINIPVDGKSPQLILQRQGKVSPFQ KDQLTLTLNQLLTGRALGSWPGSSLNLLPCPAWG S*ELVRPLDWPVWPLRVVPWNTPLLLQSLERPS CRPCQTH*NDQDRFR*GCGSNQEVGALGSSLG* PLELEAVGLQGPPCSGHIYCRDSFHSLSFC*PNG QFSGRKMEVYRLFRNRNRLNKGLKLKRLKLKGK GCC**ISGYYLLY*ENIIFTVRILLFRAFSVPD IALSALHVLAH*IFTTMLRSSHYYFCSYKCETEA HRGEKTQPESPSW*LGKLGFRSKLDCLYNPYFP PVFRASKCVRIGKHCNKWLDFLMSFFPLIVFLA PFQKTRISDFLII*VLKHPKYMGHTKIQESPVAY SLFPIGTSSHHTFKR*FCLPNAAY*MLCSYSQG ILCSVQRDS*TGKGLGIHPPYCDLPKT*SSARY TVVLIKYLMNTGSCACVPTFIRSSLLPKLHENRI LTVKILY*VP*QKRHLIFQRNAYVCIHIFVYAY ARILV*REFTP*MISSVSQCESC*IF*E*KHLP STNYKAG*QLTPFEIQWKPKS*VLTEFASQFGKL NLAFKDHRKSGWRN*GLFWQWVQEGGQNNPVAI GFEKRNYLMQSPEGKSQTHGLKPWRRKFGSNVM KRFLSQNFPAMVWPDSIKIKNNKYNGKKSPLIEC LLGAKHYAKLFIILFNCYSNFEYASFTIL*VEK RSAPKKLELKQQLILPAPALAEASGKDLNDTYW RITSTRGFKQTK*IITSVVPC*LYVLRQRKATLR VS*PLLRKFTFILRVNYSGVLRCAKGKET*IHQ VKSC*TLS*AFFERLAYTKPCGRCFPAISHLIL TANL*NMALSSESHRSLVVYSRSYQLVNSRPRLE TQDSLIPEPSLLVLRLVILSPKSKGMTFIRGAL LTIAAILTLKMIQNYQR**IRMT*LSIISLALY CVCIAITGK*SSS*LLC*TMSLLFTNLS*SKAVT MITLHIWES*VNYVSLCVYIYVCMYLFSNCDLI FKYSYCHFCD*KTTHEETS*NFPIEEK*HLGNL SCFTTVLIFLMICVAWNVFAQCEGFSLLNFSV*V FSLR**TISKVTYWSWWLFLYRQLL*DNDGALS MFNKCADGG

Frame 2

IWCS*WAGQSGPSSPERWSDPLGCSVDACATDV QA*GGSCPGRWQQ*QQQPPAPSWPRPSLQPLRP WTLPQLHWRTPPHAPNSASGHVSARHPHPAARW GSASSQMAVSAVRCALSSLGTTARRLPSVTPTGA STVTTAGTARGTQ*ECVHMLWVRWRHGTGTA*P TQAPGALAPPAAAWGSPLGSPMLTPSAGLSKRA ASATCGHAMWTSIHSLRQGRSVWLCTSQRHP*TS HLRAASAHAPINPSTVEFAWTIGAASPTSLRLS TCPSSVLMGLASPARSYGLMPASVT*AVGIPMT SLLTWNPTLTSQKLPTRQAQILGLGD*PNACEAV SPYGQ*LFTNEP*LP*SGPLASISVSNHSNDA* WCCSGPCYEFSP*YHSASTLKKNACL*LFWTTP KPDPAFPSH*KSCWILPKSQEMESGRLLISLISS LDAKPQDSLGPFR*IDGIWNNRIIYYLEPAKRY CNG*F*RQRTKTILIPNMYAPQGHQTFAK*VE* LLNFDF*WKVVSINLGIVEVKFLFTPTL*RVQIR FVPVRNKI**TFLLMGKAPS*YSRDRERSARFR RTN*LSH*ISC*LAGLWAVGQALP*IFSLVLLG VHRNW*GLWTGLSGP*EWCPGTLLYSYRALRDPA ADHARPTEMTKTGSGRGVGQTKKWVPLVAAWGD L*SWRLWDSRGPRVQDTSIAETHFTAFRSADQM ASFLVGRWRFTGCLETEIDLIKV*S*RG*S*KEK VVVNEYQAIIYCIRKI*YLLLEFFYLGPFLCQT LLSVLCMY*LTESSRQC*EVPIIISVLTNVKRK LIEVRKLNQSHPVGDWES*DSDRNWTVFITHIFP LFLELPNVSE*ENIAINGLIF*CHFSLL*SF*L LFKRREYLIF**FRCLSIQNTWDTQKSRNPL*L IPSFPSEPALITHLKDDSVYPMLHIECCVVIHRE FCAVCREIPKREKDWEYILLTVTSPKPSPVQGI QWCSLNT**IQEAVHVFLLLFEALFFQSYMKIE F*QSKFYIKCLSKRDI*YFKEMHMYVYIYLCMRM QEFLYKENSLHE*SLL*VSVNHVRFSESENTCH LQITRLDNSSLHLKFSGNPRARFLLNLHLNLGN *T*LSKIIGSLVGETRDYSGNGCRKVVRITQSPL VLRNGTILCRARRASLRPMG*SHGEGNLDPM** SAF*VRISLQWCGLIQ*KLRIINIMEKNLH*LS VYLVPSTMLSCSLFYLIVTAILSMHLSLFYKWKR EVPPKS*SSNSSLFYQPLLLRRPLEKT*MTPIG ELHLQGASNRPNRSSPLWSLVNYMF*DKGKLP* GLVNLC*GNLHSYLE*ITQVCLGVQKGRRPEFTK LNLAKPYHKHFLSA*HTPSLVEGAFLPYLI*SS QQTYRIWHYHLSLTEV*SCTQGLTS**TADQDW KPRIV*YLSHLFLCYA*LFCPPNQKA*PL*EALY *Q*LQF*L*K*FRIIKDSRFE*HDCL*SR*PCT VCA*QLQGSNLAPDYYVELCRCFLQTCLDPKQS Q**PCISGNHKSTMYPCVCIYMYVCIYFQTVI*Y LNIPTAIFVTEKLHMRKRLRIFQ*RKNNTWAIC HVSQQFSFFS*FV*RGMCLLNVKGFHCSISLCK SFP*GNKPSAKSHTGVGGFSCTGSCYETMMEH*A CSINVQMVE

Sequence WISP1.aAug05

ATATCTGGTGCTCCTGATGGGCCGGCCAGTCTGGGCCCAGCTCCCCCGAGAGGTGGTCGGATCCTCTGGGCTGCTCGGTCGATGCCTGTGCCACTGACGT CCAGGCATGAGGTGGTTCCTGCCCTGGACGCTGGCAGCAGTGACAGCAGCAGCCGCCAGCACCGTCCTGGCCACGGCCCTCTCTCCAGCCCCTACGACCA TGGACTTTACCCCAGCTCCACTGGAGGACACCTCCTCACGCCCCCAATTCTGCAAGTGGCCATGTGAGTGCCCGCCATCCCCACCCCGCTGCCCGCTGGG GGTCAGCCTCATCACAGATGGCTGTGAGTGCTGTAAGATGTGCGCTCAGCAGCTTGGGGACAACTGCACGGAGGCTGCCATCTGTGACCCCCACCGGGGC CTCTACTGTGACTACAGCGGGGACCGCCCGAGGTACGCAATAGGAGTGTGTGCACAGGTGGTCGGTGTGGGCTGCGTCCTGGATGGGGTGCGCTACAACA ACGGCCAGTCCTTCCAGCCTAACTGCAAGTACAACTGCACGTGCATCGACGGCGCGGTGGGCTGCACACCACTGTGCCTCCGAGTGCGCCCCCCGCGTCT CTGGTGCCCCCACCCGCGGCGCGTGAGCATACCTGGCCACTGCTGTGAGCAGTGGGTATGTGAGGACGACGCCAAGAGGCCACGCAAGACCGCACCCCGT GACACAGGAGCCTTCGATGCTGTGGGTGAGGTGGAGGCATGGCACAGGAACTGCATAGCCTACACAAGCCCCTGGAGCCCTTGCTCCACCAGCTGCGGCC TGGGGGTCTCCACTCGGATCTCCAATGTTAACGCCCAGTGCTGGCCTGAGCAAGAGAGCCGCCTCTGCAACTTGCGGCCATGCGATGTGGACATCCATAC ACTCATTAAGGCAGGGAAGAAGTGTCTGGCTGTGTACCAGCCAGAGGCATCCATGAACTTCACACTTGCGGGCTGCATCAGCACACGCTCCTATCAACCC AAGTACTGTGGAGTTTGCATGGACAATAGGTGCTGCATCCCCTACAAGTCTAAGACTATCGACGTGTCCTTCCAGTGTCCTGATGGGCTTGGCTTCTCCC GCCAGGTCCTATGGATTAATGCCTGCTTCTGTAACCTGAGCTGTAGGAATCCCAATGACATCTTTGCTGACTTGGAATCCTACCCTGACTTCTCAGAAAT TGCCAACTAGGCAGGCACAAATCTTGGGTCTTGGGGACTAACCCAATGCCTGTGAAGCAGTCAGCCCTTATGGCCAATAACTTTTCACCAATGAGCCTTA GTTACCCTGATCTGGACCCTTGGCCTCCATTTCTGTCTCTAACCATTCAAATGACGCCTGATGGTGCTGCTCAGGCCCATGCTATGAGTTTTCTCCTTGA TATCATTCAGCATCTACTCTAAAGAAAAATGCCTGTCTCTAGCTGTTCTGGACTACACCCAAGCCTGATCCAGCCTTTCCAAGTCACTAGAAGTCCTGCT GGATCTTGCCTAAATCCCAAGAAATGGAATCAGGTAGACTTTTAATATCACTAATTTCTTCTTTAGATGCCAAACCACAAGACTCTTTGGGTCCATTCAG ATGAATAGATGGAATTTGGAACAATAGAATAATCTATTATTTGGAGCCTGCCAAGAGGTACTGTAATGGGTAATTCTGACGTCAGCGCACCAAAACTATC CTGATTCCAAATATGTATGCACCTCAAGGTCATCAAACATTTGCCAAGTGAGTTGAATAGTTGCTTAATTTTGATTTTTAATGGAAAGTTGTATCCATTA ACCTGGGCATTGTTGAGGTTAAGTTTCTCTTCACCCCTACACTGTGAAGGGTACAGATTAGGTTTGTCCCAGTCAGAAATAAAATTTGATAAACATTCCT GTTGATGGGAAAAGCCCCCAGTTAATACTCCAGAGACAGGGAAAGGTCAGCCCGTTTCAGAAGGACCAATTGACTCTCACACTGAATCAGCTGCTGACTG GCAGGGCTTTGGGCAGTTGGCCAGGCTCTTCCTTGAATCTTCTCCCTTGTCCTGCTTGGGGTTCATAGGAATTGGTAAGGCCTCTGGACTGGCCTGTCTG GCCCCTGAGAGTGGTGCCCTGGAACACTCCTCTACTCTTACAGAGCCTTGAGAGACCCAGCTGCAGACCATGCCAGACCCACTGAAATGACCAAGACAGG TTCAGGTAGGGGTGTGGGTCAAACCAAGAAGTGGGTGCCCTTGGTAGCAGCCTGGGGTGACCTCTAGAGCTGGAGGCTGTGGGACTCCAGGGGCCCCCGT GTTCAGGACACATCTATTGCAGAGACTCATTTCACAGCCTTTCGTTCTGCTGACCAAATGGCCAGTTTTCTGGTAGGAAGATGGAGGTTTACCGGTTGTT TAGAAACAGAAATAGACTTAATAAAGGTTTAAAGCTGAAGAGGTTGAAGCTAAAAGGAAAAGGTTGTTGTTAATGAATATCAGGCTATTATTTATTGTAT TAGGAAAATATAATATTTACTGTTAGAATTCTTTTATTTAGGGCCTTTTCTGTGCCAGACATTGCTCTCAGTGCTTTGCATGTATTAGCTCACTGAATCT TCACGACAATGTTGAGAAGTTCCCATTATTATTTCTGTTCTTACAAATGTGAAACGGAAGCTCATAGAGGTGAGAAAACTCAACCAGAGTCACCCAGTTG GTGACTGGGAAAGTTAGGATTCAGATCGAAATTGGACTGTCTTTATAACCCATATTTTCCCCCTGTTTTTAGAGCTTCCAAATGTGTCAGAATAGGAAAA CATTGCAATAAATGGCTTGATTTTTTAATGTCATTTTTCCCTCTTATAGTCTTTCTAGCTCCTTTTCAAAAGACGAGAATATCTGATTTTCTGATAATTT AGGTGCTTAAGCATCCAAAATACATGGGACACACAAAAATCCAGGAATCCCCTGTAGCTTATTCCCTCTTTCCCATCGGAACCAGCTCTCATCACACATT TAAAAGATGATTCTGTTTACCCAATGCTGCATATTGAATGTTGTGTAGTTATTCACAGGGAATTCTGTGCAGTGTGCAGAGAGATTCCTAAACGGGAAAA GGACTGGGAATACATCCTCCTTACTGTGACCTCCCCAAAACCTAGTCCAGTGCAAGGTATACAGTGGTGCTCATTAAATACTTGATGAATACAGGAAGCT GTGCATGTGTTCCTACTTTTATTCGAAGCTCTCTTCTTCCAAAGCTACATGAAAATAGAATTTTAACAGTCAAAATTTTATATTAAGTGCCTTAGCAAAA GAGACATTTAATATTTCAAAGAAATGCATATGTATGTATACATATATTTGTGTATGCGTATGCAAGAATTCTTGTATAAAGAGAATTCACTCCATGAATG ATCTCTTCTGTAAGTCAGTGTGAATCATGTTAGATTTTCTGAGAGTGAAAACACCTGCCATCTACAAATTACAAGGCTGGATAACAGCTCACTCCATTTG AAATTCAGTGGAAACCCAAGAGCTAGGTTCTTACTGAATTTGCATCTCAATTTGGGAAACTGAACTTAGCTTTCAAAGATCATAGGAAGTCTGGTTGGAG AAACTAGGGATTATTCTGGCAATGGGTGCAGGAAGGTGGTCAGAATAACCCAGTCGCCATTGGTTTTGAGAAACGGAACTATCTTATGCAGAGCCCGGAG GGCAAGTCTCAGACCCATGGGTTGAAGCCATGGAGAAGGAAATTTGGATCCAATGTAATGAAGCGCTTTCTAAGTCAGAATTTCCCTGCAATGGTGTGGC CTGATTCAATAAAAATTAAGAATAATAAATATAATGGAAAAAAATCTCCACTGATTGAGTGTTTACTTGGTGCCAAGCACTATGCTAAGTTGTTCATTAT TTTATTTAATTGTTACAGCAATTTTGAGTATGCATCTTTCACTATTTTATAAGTGGAAAAGAGAAGTGCCCCCAAAAAGTTAGAGCTCAAACAGCAGCTT ATTCTACCAGCCCCTGCTCTTGCGGAGGCCTCTGGAAAAGACCTGAATGACACCTATTGGAGAATTACATCTACAAGGGGCTTCAAACAGACCAAATAGA TCATCACCTCTGTGGTCCCTTGTTAACTATATGTTCTGAGACAAAGGAAAGCTACCCTAAGGGTTAGTTAACCTTTGCTGAGGAAATTTACATTCATACT TAGAGTGAATTACTCAGGTGTGCTTAGGTGTGCAAAAGGGAAGGAGACCTGAATTCACCAAGTTAAATCTTGCTAAACCTTATCATAAGCATTTTTTGAG CGCTTAGCATACACCAAGCCTTGTGGAAGGTGCTTTCCTGCCATATCTCATTTAATCCTCACAGCAAACCTATAGAATATGGCATTATCATCTGAGTCTC ACAGAAGTTTAGTCGTGTACTCAAGGTCTTACCAGCTAGTGAACAGCAGACCAAGACTGGAAACCCAGGATAGTCTGATACCTGAGCCATCTCTTCTTGT GCTACGCCTAGTTATTCTGTCCCCCAAATCAAAAGGCATGACCTTTATAAGAGGCGCTTTACTGACAATAGCTGCAATTTTAACTTTGAAAATGATTCAG AATTATCAAAGATAGTAGATTCGAATGACATGATTGTCTATAATCTCGCTAGCCTTGTACTGTGTGTGCATAGCAATTACAGGGAAGTAATCTAGCTCCT GACTATTATGTTGAACTATGTCGCTGCTTTTTACAAACTTGTCTTGATCCAAAGCAGTCACAATGATAACCCTGCATATCTGGGAATCATAAGTCAACTA TGTATCCCTGTGTGTGTATATATATGTATGTATGTATCTATTTTCAAACTGTGATTTAATATTTAAATATTCCTACTGCCATTTTTGTGACTGAAAAACT ACACATGAGGAAACGTCTTAGAATTTTCCAATAGAGGAAAAATAACACTTGGGCAATCTGTCATGTTTCACAACAGTTCTCATTTTTCTCATGATTTGTG TAGCGTGGAATGTGTTTGCTCAATGTGAAGGGTTTTCATTGCTCAATTTCTCTGTGTAAGTCTTTTCCTTAAGGTAATAAACCATCAGCAAAGTCACATA CTGGAGTTGGTGGCTTTTCTTGTACAGGCAGTTGTTATGAGACAATGATGGAGCATTGAGCATGTTCAATAAATGTGCAGATGGTGGAAA

WISP1.u.f.4 (forward primer)
WISP1.u.r.4 (reverse primer)
Single exon skipping

Frame 0

ISGAPDGPASLGPAPPRGGRILWAARSMPVPLT SRHEVVPALDAGSSDSSSRQHRPGHGPLSSPYDH GLYPSSTGGHLLTPPILQVAM*VPAIPTPLPAG GQPHHRWL*VL*DVRSAAWGQLHGGCHL*PPPG PLL*LQRGPPEVRNRSVCTGGRCGLRPGWGALQQ RPVLPA*LQVQLHVHRRRGGLHTTVPPSAPPAS LVPPPAAREHTWPLL*AVGM*GRRQEATQDRTP *HRSLRCCG*GGGMAQELHSLHKPLEPLLHQLRP GGLHSDLQC*RPVLA*AREPPLQLAAMRCGHPY TH*GREEVSGCVPARGIHELHTCGLHQHTLLST QVLWSLHGQ*VLHPLQV*DYRRVLPVS*WAWLLP PGPMD*CLLL*PEL*ESQ*HLC*LGILP*LLRN CQLGRHKSWVLGTNPMPVKQSALMANNFSPMSL SYPDLDPWPPFLSLTIQMTPDGAAQAHAMSFLLD IIQHLL*RKMPVSSCSGLHPSLIQPFQVTRSPA GSCLNPKKWNQVDF*YH*FLL*MPNHKTLWVHS DE*MEFGTIE*SIIWSLPRGTVMGNSDVSAPKLS *FQICMHLKVIKHLPSELNSCLILIFNGKLYPL TWALLRLSFSSPLHCEGYRLGLSQSEIKFDKHS C*WEKPPVNTPETGKGQPVSEGPIDSHTESAADW QGFGQLARLFLESSPLSCLGFIGIGKASGLACL APESGALEHSSTLTEP*ETQLQTMPDPLK*PRQ VQVGVWVKPRSGCPW*QPGVTSRAGGCGTPGAPV FRTHLLQRLISQPFVLLTKWPVFW*EDGGLPVV *KQK*T**RFKAEEVEAKRKRLLLMNIRLLFIV LGKYNIYC*NSFI*GLFCARHCSQCFACISSLNL HDNVEKFPLLFLFLQM*NGSS*R*ENSTRVTQL VTGKVRIQIEIGLSL*PIFSPCF*SFQMCQNRK TLQ*MA*FFNVIFPSYSLSSSFSKDENI*FSDNL GA*ASKIHGTHKNPGIPCSLFPLSHRNQLSSHI *KMILFTQCCILNVV*LFTGNSVQCAERFLNGK RTGNTSSLL*PPQNLVQCKVYSGAH*ILDEYRKL CMCSYFYSKLSSSKAT*K*NFNSQNFILSALAK ETFNISKKCICMYTYICVCVCKNSCIKRIHSMN DLFCKSV*IMLDFLRVKTPAIYKLQGWITAHSI* NSVETQELGSY*ICISIWETELSFQRS*EVWLE KLGIILAMGAGRWSE*PSRHWF*ETELSYAEPG GQVSDPWVEAMEKEIWIQCNEALSKSEFPCNGVA *FNKN*E**I*WKKISTD*VFTWCQALC*VVHY FI*LLQQF*VCIFHYFISGKEKCPQKVRAQTAA YSTSPCSCGGLWKRPE*HLLENYIYKGLQTDQID HHLCGPLLTICSETKESYPKG*LTFAEEIYIHT *SELLRCA*VCKREGDLNSPS*ILLNLIISIF* ALSIHQALWKVLSCHISFNPHSKPIEYGIII*VS QKFSRVLKVLPASEQQTKTGNPG*SDT*AISSC ATPSYSVPQIKRHDLYKRRFTDNSCNFNFENDS ELSKIVDSNDMIVYNLASLVLCVHSNYREVI*LL TIMLNYVAAFYKLVLIQSSHNDNPAYLGIISQL CIPVCVYICMYVSIFKL*FNI*IFLLPFL*LKN YT*GNVLEFSNRGKITLGQSVMFHNSSHFSHDLC SVECVCSM*RVFIAQFLCVSLFLKVINHQQSHI LELVAFLVQAVVMRQ*WSIEHVQ*MCRWWK

Frame 1

YLVLLMGRPVWAQLPREVVGSSGLLGRCLCH*R PGMRWFLPWTLAAVTAAAASTVLATALSPAPTT MDFTPAPLEDTSSRPQFCKWPCECPPSPPRCPLG VSLITDGCECCKMCAQQLGDNCTEAAICDPHRG LYCDYSGDRPRYAIGVCAQVVGVGCVLDGVRYN NGQSFQPNCKYNCTCIDGAVGCTPLCLRVRPPRL WCPHPRRVSIPGHCCEQWVCEDDAKRPRKTAPR DTGAFDAVGEVEAWHRNCIAYTSPWSPCSTSCG LGVSTRISNVNAQCWPEQESRLCNLRPCDVDIHT LIKAGKKCLAVYQPEASMNFTLAGCISTRSYQP KYCGVCMDNRCCIPYKSKTIDVSFQCPDGLGFS RQVLWINACFCNLSCRNPNDIFADLESYPDFSEI AN*AGTNLGSWGLTQCL*SSQPLWPITFHQ*AL VTLIWTLGLHFCL*PFK*RLMVLLRPML*VFSL ISFSIYSKEKCLSLAVLDYTQA*SSLSKSLEVLL DLA*IPRNGIR*TFNITNFFFRCQTTRLFGSIQ MNRWNLEQ*NNLLFGACQEVL*WVILTSAHQNY PDSKYVCTSRSSNICQVS*IVA*F*FLMESCIH* PGHC*G*VSLHPYTVKGTD*VCPSQK*NLINIP VDGKSPQLILQRQGKVSPFQKDQLTLTLNQLLT GRALGSWPGSSLNLLPCPAWGS*ELVRPLDWPVW PLRVVPWNTPLLLQSLERPSCRPCQTH*NDQDR FR*GCGSNQEVGALGSSLG*PLELEAVGLQGPP CSGHIYCRDSFHSLSFC*PNGQFSGRKMEVYRLF RNRNRLNKGLKLKRLKLKGKGCC**ISGYYLLY *ENIIFTVRILLFRAFSVPDIALSALHVLAH*I FTTMLRSSHYYFCSYKCETEAHRGEKTQPESPSW *LGKLGFRSKLDCLYNPYFPPVFRASKCVRIGK HCNKWLDFLMSFFPLIVFLAPFQKTRISDFLII *VLKHPKYMGHTKIQESPVAYSLFPIGTSSHHTF KR*FCLPNAAY*MLCSYSQGILCSVQRDS*TGK GLGIHPPYCDLPKT*SSARYTVVLIKYLMNTGS CACVPTFIRSSLLPKLHENRILTVKILY*VP*QK RHLIFQRNAYVCIHIFVYAYARILV*REFTP*M ISSVSQCESC*IF*E*KHLPSTNYKAG*QLTPF EIQWKPKS*VLTEFASQFGKLNLAFKDHRKSGWR N*GLFWQWVQEGGQNNPVAIGFEKRNYLMQSPE GKSQTHGLKPWRRKFGSNVMKRFLSQNFPAMVW PDSIKIKNNKYNGKKSPLIECLLGAKHYAKLFII LFNCYSNFEYASFTIL*VEKRSAPKKLELKQQL ILPAPALAEASGKDLNDTYWRITSTRGFKQTK* IITSVVPC*LYVLRQRKATLRVS*PLLRKFTFIL RVNYSGVLRCAKGKET*IHQVKSC*TLS*AFFE RLAYTKPCGRCFPAISHLILTANL*NMALSSES HRSLVVYSRSYQLVNSRPRLETQDSLIPEPSLLV LRLVILSPKSKGMTFIRGALLTIAAILTLKMIQ NYQR**IRMT*LSIISLALYCVCIAITGK*SSS *LLC*TMSLLFTNLS*SKAVTMITLHIWES*VNY VSLCVYIYVCMYLFSNCDLIFKYSYCHFCD*KT THEETS*NFPIEEK*HLGNLSCFTTVLIFLMIC VAWNVFAQCEGFSLLNFSV*VFSLR**TISKVTY WSWWLFLYRQLL*DNDGALSMFNKCADGG

Frame 2

IWCS*WAGQSGPSSPERWSDPLGCSVDACATDV QA*GGSCPGRWQQ*QQQPPAPSWPRPSLQPLRP WTLPQLHWRTPPHAPNSASGHVSARHPHPAARW GSASSQMAVSAVRCALSSLGTTARRLPSVTPTGA STVTTAGTARGTQ*ECVHRWSVWAASWMGCATT TASPSSLTASTTARASTARWAAHHCASECAPRV SGAPTRGA*AYLATAVSSGYVRTTPRGHARPHPV TQEPSMLWVRWRHGTGTA*PTQAPGALAPPAAA WGSPLGSPMLTPSAGLSKRAASATCGHAMWTSI HSLRQGRSVWLCTSQRHP*TSHLRAASAHAPINP STVEFAWTIGAASPTSLRLSTCPSSVLMGLASP ARSYGLMPASVT*AVGIPMTSLLTWNPTLTSQK LPTRQAQILGLGD*PNACEAVSPYGQ*LFTNEP* LP*SGPLASISVSNHSNDA*WCCSGPCYEFSP* YHSASTLKKNACL*LFWTTPKPDPAFPSH*KSC WILPKSQEMESGRLLISLISSLDAKPQDSLGPFR *IDGIWNNRIIYYLEPAKRYCNG*F*RQRTKTI LIPNMYAPQGHQTFAK*VE*LLNFDF*WKVVSI NLGIVEVKFLFTPTL*RVQIRFVPVRNKI**TFL LMGKAPS*YSRDRERSARFRRTN*LSH*ISC*L AGLWAVGQALP*IFSLVLLGVHRNW*GLWTGLS GP*EWCPGTLLYSYRALRDPAADHARPTEMTKTG SGRGVGQTKKWVPLVAAWGDL*SWRLWDSRGPR VQDTSIAETHFTAFRSADQMASFLVGRWRFTGC LETEIDLIKV*S*RG*S*KEKVVVNEYQAIIYCI RKI*YLLLEFFYLGPFLCQTLLSVLCMY*LTES SRQC*EVPIIISVLTNVKRKLIEVRKLNQSHPV GDWES*DSDRNWTVFITHIFPLFLELPNVSE*EN IAINGLIF*CHFSLL*SF*LLFKRREYLIF**F RCLSIQNTWDTQKSRNPL*LIPSFPSEPALITH LKDDSVYPMLHIECCVVIHREFCAVCREIPKREK DWEYILLTVTSPKPSPVQGIQWCSLNT**IQEA VHVFLLLFEALFFQSYMKIEF*QSKFYIKCLSK RDI*YFKEMHMYVYIYLCMRMQEFLYKENSLHE* SLL*VSVNHVRFSESENTCHLQITRLDNSSLHL KFSGNPRARFLLNLHLNLGN*T*LSKIIGSLVG ETRDYSGNGCRKVVRITQSPLVLRNGTILCRARR ASLRPMG*SHGEGNLDPM**SAF*VRISLQWCG LIQ*KLRIINIMEKNLH*LSVYLVPSTMLSCSL FYLIVTAILSMHLSLFYKWKREVPPKS*SSNSSL FYQPLLLRRPLEKT*MTPIGELHLQGASNRPNR SSPLWSLVNYMF*DKGKLP*GLVNLC*GNLHSY LE*ITQVCLGVQKGRRPEFTKLNLAKPYHKHFLS A*HTPSLVEGAFLPYLI*SSQQTYRIWHYHLSL TEV*SCTQGLTS**TADQDWKPRIV*YLSHLFL CYA*LFCPPNQKA*PL*EALY*Q*LQF*L*K*FR IIKDSRFE*HDCL*SR*PCTVCA*QLQGSNLAP DYYVELCRCFLQTCLDPKQSQ**PCISGNHKST MYPCVCIYMYVCIYFQTVI*YLNIPTAIFVTEKL HMRKRLRIFQ*RKNNTWAICHVSQQFSFFS*FV *RGMCLLNVKGFHCSISLCKSFP*GNKPSAKSH TGVGGFSCTGSCYETMMEH*ACSINVQMVE