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Sequences

Sequence WISP1.dAug05

ATATCTGGTGCTCCTGATGGGCCGGCCAGTCTGGGCCCAGCTCCCCCGAGAGGTGGTCGGATCCTCTGGGCTGCTCGGTCGATGCCTGTGCCACTGACGT CCAGGCATGAGGTGGTTCCTGCCCTGGACGCTGGCAGCAGTGACAGCAGCAGCCGCCAGCACCGTCCTGGCCACGGCCCTCTCTCCAGCCCCTACGACCA TGGACTTTACCCCAGCTCCACTGGAGGACACCTCCTCACGCCCCCAATTCTGCAAGTGGCCATGTGAGTGCCCGCCATCCCCACCCCGCTGCCCGCTGGG GGTCAGCCTCATCACAGATGGCTGTGAGTGCTGTAAGATGTGCGCTCAGCAGCTTGGGGACAACTGCACGGAGGCTGCCATCTGTGACCCCCACCGGGGC CTCTACTGTGACTACAGCGGGGACCGCCCGAGGTACGCAATAGGAGTGTGTGCACGCAGGGAAGAAGTGTCTGGCTGTGTACCAGCCAGAGGCATCCATG AACTTCACACTTGCGGGCTGCATCAGCACACGCTCCTATCAACCCAAGTACTGTGGAGTTTGCATGGACAATAGGTGCTGCATCCCCTACAAGTCTAAGA CTATCGACGTGTCCTTCCAGTGTCCTGATGGGCTTGGCTTCTCCCGCCAGGTCCTATGGATTAATGCCTGCTTCTGTAACCTGAGCTGTAGGAATCCCAA TGACATCTTTGCTGACTTGGAATCCTACCCTGACTTCTCAGAAATTGCCAACTAGGCAGGCACAAATCTTGGGTCTTGGGGACTAACCCAATGCCTGTGA AGCAGTCAGCCCTTATGGCCAATAACTTTTCACCAATGAGCCTTAGTTACCCTGATCTGGACCCTTGGCCTCCATTTCTGTCTCTAACCATTCAAATGAC GCCTGATGGTGCTGCTCAGGCCCATGCTATGAGTTTTCTCCTTGATATCATTCAGCATCTACTCTAAAGAAAAATGCCTGTCTCTAGCTGTTCTGGACTA CACCCAAGCCTGATCCAGCCTTTCCAAGTCACTAGAAGTCCTGCTGGATCTTGCCTAAATCCCAAGAAATGGAATCAGGTAGACTTTTAATATCACTAAT TTCTTCTTTAGATGCCAAACCACAAGACTCTTTGGGTCCATTCAGATGAATAGATGGAATTTGGAACAATAGAATAATCTATTATTTGGAGCCTGCCAAG AGGTACTGTAATGGGTAATTCTGACGTCAGCGCACCAAAACTATCCTGATTCCAAATATGTATGCACCTCAAGGTCATCAAACATTTGCCAAGTGAGTTG AATAGTTGCTTAATTTTGATTTTTAATGGAAAGTTGTATCCATTAACCTGGGCATTGTTGAGGTTAAGTTTCTCTTCACCCCTACACTGTGAAGGGTACA GATTAGGTTTGTCCCAGTCAGAAATAAAATTTGATAAACATTCCTGTTGATGGGAAAAGCCCCCAGTTAATACTCCAGAGACAGGGAAAGGTCAGCCCGT TTCAGAAGGACCAATTGACTCTCACACTGAATCAGCTGCTGACTGGCAGGGCTTTGGGCAGTTGGCCAGGCTCTTCCTTGAATCTTCTCCCTTGTCCTGC TTGGGGTTCATAGGAATTGGTAAGGCCTCTGGACTGGCCTGTCTGGCCCCTGAGAGTGGTGCCCTGGAACACTCCTCTACTCTTACAGAGCCTTGAGAGA CCCAGCTGCAGACCATGCCAGACCCACTGAAATGACCAAGACAGGTTCAGGTAGGGGTGTGGGTCAAACCAAGAAGTGGGTGCCCTTGGTAGCAGCCTGG GGTGACCTCTAGAGCTGGAGGCTGTGGGACTCCAGGGGCCCCCGTGTTCAGGACACATCTATTGCAGAGACTCATTTCACAGCCTTTCGTTCTGCTGACC AAATGGCCAGTTTTCTGGTAGGAAGATGGAGGTTTACCGGTTGTTTAGAAACAGAAATAGACTTAATAAAGGTTTAAAGCTGAAGAGGTTGAAGCTAAAA GGAAAAGGTTGTTGTTAATGAATATCAGGCTATTATTTATTGTATTAGGAAAATATAATATTTACTGTTAGAATTCTTTTATTTAGGGCCTTTTCTGTGC CAGACATTGCTCTCAGTGCTTTGCATGTATTAGCTCACTGAATCTTCACGACAATGTTGAGAAGTTCCCATTATTATTTCTGTTCTTACAAATGTGAAAC GGAAGCTCATAGAGGTGAGAAAACTCAACCAGAGTCACCCAGTTGGTGACTGGGAAAGTTAGGATTCAGATCGAAATTGGACTGTCTTTATAACCCATAT TTTCCCCCTGTTTTTAGAGCTTCCAAATGTGTCAGAATAGGAAAACATTGCAATAAATGGCTTGATTTTTTAATGTCATTTTTCCCTCTTATAGTCTTTC TAGCTCCTTTTCAAAAGACGAGAATATCTGATTTTCTGATAATTTAGGTGCTTAAGCATCCAAAATACATGGGACACACAAAAATCCAGGAATCCCCTGT AGCTTATTCCCTCTTTCCCATCGGAACCAGCTCTCATCACACATTTAAAAGATGATTCTGTTTACCCAATGCTGCATATTGAATGTTGTGTAGTTATTCA CAGGGAATTCTGTGCAGTGTGCAGAGAGATTCCTAAACGGGAAAAGGACTGGGAATACATCCTCCTTACTGTGACCTCCCCAAAACCTAGTCCAGTGCAA GGTATACAGTGGTGCTCATTAAATACTTGATGAATACAGGAAGCTGTGCATGTGTTCCTACTTTTATTCGAAGCTCTCTTCTTCCAAAGCTACATGAAAA TAGAATTTTAACAGTCAAAATTTTATATTAAGTGCCTTAGCAAAAGAGACATTTAATATTTCAAAGAAATGCATATGTATGTATACATATATTTGTGTAT GCGTATGCAAGAATTCTTGTATAAAGAGAATTCACTCCATGAATGATCTCTTCTGTAAGTCAGTGTGAATCATGTTAGATTTTCTGAGAGTGAAAACACC TGCCATCTACAAATTACAAGGCTGGATAACAGCTCACTCCATTTGAAATTCAGTGGAAACCCAAGAGCTAGGTTCTTACTGAATTTGCATCTCAATTTGG GAAACTGAACTTAGCTTTCAAAGATCATAGGAAGTCTGGTTGGAGAAACTAGGGATTATTCTGGCAATGGGTGCAGGAAGGTGGTCAGAATAACCCAGTC GCCATTGGTTTTGAGAAACGGAACTATCTTATGCAGAGCCCGGAGGGCAAGTCTCAGACCCATGGGTTGAAGCCATGGAGAAGGAAATTTGGATCCAATG TAATGAAGCGCTTTCTAAGTCAGAATTTCCCTGCAATGGTGTGGCCTGATTCAATAAAAATTAAGAATAATAAATATAATGGAAAAAAATCTCCACTGAT TGAGTGTTTACTTGGTGCCAAGCACTATGCTAAGTTGTTCATTATTTTATTTAATTGTTACAGCAATTTTGAGTATGCATCTTTCACTATTTTATAAGTG GAAAAGAGAAGTGCCCCCAAAAAGTTAGAGCTCAAACAGCAGCTTATTCTACCAGCCCCTGCTCTTGCGGAGGCCTCTGGAAAAGACCTGAATGACACCT ATTGGAGAATTACATCTACAAGGGGCTTCAAACAGACCAAATAGATCATCACCTCTGTGGTCCCTTGTTAACTATATGTTCTGAGACAAAGGAAAGCTAC CCTAAGGGTTAGTTAACCTTTGCTGAGGAAATTTACATTCATACTTAGAGTGAATTACTCAGGTGTGCTTAGGTGTGCAAAAGGGAAGGAGACCTGAATT CACCAAGTTAAATCTTGCTAAACCTTATCATAAGCATTTTTTGAGCGCTTAGCATACACCAAGCCTTGTGGAAGGTGCTTTCCTGCCATATCTCATTTAA TCCTCACAGCAAACCTATAGAATATGGCATTATCATCTGAGTCTCACAGAAGTTTAGTCGTGTACTCAAGGTCTTACCAGCTAGTGAACAGCAGACCAAG ACTGGAAACCCAGGATAGTCTGATACCTGAGCCATCTCTTCTTGTGCTACGCCTAGTTATTCTGTCCCCCAAATCAAAAGGCATGACCTTTATAAGAGGC GCTTTACTGACAATAGCTGCAATTTTAACTTTGAAAATGATTCAGAATTATCAAAGATAGTAGATTCGAATGACATGATTGTCTATAATCTCGCTAGCCT TGTACTGTGTGTGCATAGCAATTACAGGGAAGTAATCTAGCTCCTGACTATTATGTTGAACTATGTCGCTGCTTTTTACAAACTTGTCTTGATCCAAAGC AGTCACAATGATAACCCTGCATATCTGGGAATCATAAGTCAACTATGTATCCCTGTGTGTGTATATATATGTATGTATGTATCTATTTTCAAACTGTGAT TTAATATTTAAATATTCCTACTGCCATTTTTGTGACTGAAAAACTACACATGAGGAAACGTCTTAGAATTTTCCAATAGAGGAAAAATAACACTTGGGCA ATCTGTCATGTTTCACAACAGTTCTCATTTTTCTCATGATTTGTGTAGCGTGGAATGTGTTTGCTCAATGTGAAGGGTTTTCATTGCTCAATTTCTCTGT GTAAGTCTTTTCCTTAAGGTAATAAACCATCAGCAAAGTCACATACTGGAGTTGGTGGCTTTTCTTGTACAGGCAGTTGTTATGAGACAATGATGGAGCA TTGAGCATGTTCAATAAATGTGCAGATGGTGGAAA

WISP1.u.f.4 (forward primer)
WISP1.u.r.4 (reverse primer)
Single exon skipping

Frame 0

ISGAPDGPASLGPAPPRGGRILWAARSMPVPLT SRHEVVPALDAGSSDSSSRQHRPGHGPLSSPYDH GLYPSSTGGHLLTPPILQVAM*VPAIPTPLPAG GQPHHRWL*VL*DVRSAAWGQLHGGCHL*PPPG PLL*LQRGPPEVRNRSVCTQGRSVWLCTSQRHP* TSHLRAASAHAPINPSTVEFAWTIGAASPTSLR LSTCPSSVLMGLASPARSYGLMPASVT*AVGIP MTSLLTWNPTLTSQKLPTRQAQILGLGD*PNACE AVSPYGQ*LFTNEP*LP*SGPLASISVSNHSND A*WCCSGPCYEFSP*YHSASTLKKNACL*LFWT TPKPDPAFPSH*KSCWILPKSQEMESGRLLISLI SSLDAKPQDSLGPFR*IDGIWNNRIIYYLEPAK RYCNG*F*RQRTKTILIPNMYAPQGHQTFAK*V E*LLNFDF*WKVVSINLGIVEVKFLFTPTL*RVQ IRFVPVRNKI**TFLLMGKAPS*YSRDRERSAR FRRTN*LSH*ISC*LAGLWAVGQALP*IFSLVL LGVHRNW*GLWTGLSGP*EWCPGTLLYSYRALRD PAADHARPTEMTKTGSGRGVGQTKKWVPLVAAW GDL*SWRLWDSRGPRVQDTSIAETHFTAFRSAD QMASFLVGRWRFTGCLETEIDLIKV*S*RG*S*K EKVVVNEYQAIIYCIRKI*YLLLEFFYLGPFLC QTLLSVLCMY*LTESSRQC*EVPIIISVLTNVK RKLIEVRKLNQSHPVGDWES*DSDRNWTVFITHI FPLFLELPNVSE*ENIAINGLIF*CHFSLL*SF *LLFKRREYLIF**FRCLSIQNTWDTQKSRNPL *LIPSFPSEPALITHLKDDSVYPMLHIECCVVIH REFCAVCREIPKREKDWEYILLTVTSPKPSPVQ GIQWCSLNT**IQEAVHVFLLLFEALFFQSYMK IEF*QSKFYIKCLSKRDI*YFKEMHMYVYIYLCM RMQEFLYKENSLHE*SLL*VSVNHVRFSESENT CHLQITRLDNSSLHLKFSGNPRARFLLNLHLNL GN*T*LSKIIGSLVGETRDYSGNGCRKVVRITQS PLVLRNGTILCRARRASLRPMG*SHGEGNLDPM **SAF*VRISLQWCGLIQ*KLRIINIMEKNLH* LSVYLVPSTMLSCSLFYLIVTAILSMHLSLFYKW KREVPPKS*SSNSSLFYQPLLLRRPLEKT*MTP IGELHLQGASNRPNRSSPLWSLVNYMF*DKGKL P*GLVNLC*GNLHSYLE*ITQVCLGVQKGRRPEF TKLNLAKPYHKHFLSA*HTPSLVEGAFLPYLI* SSQQTYRIWHYHLSLTEV*SCTQGLTS**TADQ DWKPRIV*YLSHLFLCYA*LFCPPNQKA*PL*EA LY*Q*LQF*L*K*FRIIKDSRFE*HDCL*SR*P CTVCA*QLQGSNLAPDYYVELCRCFLQTCLDPK QSQ**PCISGNHKSTMYPCVCIYMYVCIYFQTVI *YLNIPTAIFVTEKLHMRKRLRIFQ*RKNNTWA ICHVSQQFSFFS*FV*RGMCLLNVKGFHCSISL CKSFP*GNKPSAKSHTGVGGFSCTGSCYETMMEH *ACSINVQMVE

Frame 1

YLVLLMGRPVWAQLPREVVGSSGLLGRCLCH*R PGMRWFLPWTLAAVTAAAASTVLATALSPAPTT MDFTPAPLEDTSSRPQFCKWPCECPPSPPRCPLG VSLITDGCECCKMCAQQLGDNCTEAAICDPHRG LYCDYSGDRPRYAIGVCARREEVSGCVPARGIH ELHTCGLHQHTLLSTQVLWSLHGQ*VLHPLQV*D YRRVLPVS*WAWLLPPGPMD*CLLL*PEL*ESQ *HLC*LGILP*LLRNCQLGRHKSWVLGTNPMPV KQSALMANNFSPMSLSYPDLDPWPPFLSLTIQMT PDGAAQAHAMSFLLDIIQHLL*RKMPVSSCSGL HPSLIQPFQVTRSPAGSCLNPKKWNQVDF*YH* FLL*MPNHKTLWVHSDE*MEFGTIE*SIIWSLPR GTVMGNSDVSAPKLS*FQICMHLKVIKHLPSEL NSCLILIFNGKLYPLTWALLRLSFSSPLHCEGY RLGLSQSEIKFDKHSC*WEKPPVNTPETGKGQPV SEGPIDSHTESAADWQGFGQLARLFLESSPLSC LGFIGIGKASGLACLAPESGALEHSSTLTEP*E TQLQTMPDPLK*PRQVQVGVWVKPRSGCPW*QPG VTSRAGGCGTPGAPVFRTHLLQRLISQPFVLLT KWPVFW*EDGGLPVV*KQK*T**RFKAEEVEAK RKRLLLMNIRLLFIVLGKYNIYC*NSFI*GLFCA RHCSQCFACISSLNLHDNVEKFPLLFLFLQM*N GSS*R*ENSTRVTQLVTGKVRIQIEIGLSL*PI FSPCF*SFQMCQNRKTLQ*MA*FFNVIFPSYSLS SSFSKDENI*FSDNLGA*ASKIHGTHKNPGIPC SLFPLSHRNQLSSHI*KMILFTQCCILNVV*LF TGNSVQCAERFLNGKRTGNTSSLL*PPQNLVQCK VYSGAH*ILDEYRKLCMCSYFYSKLSSSKAT*K *NFNSQNFILSALAKETFNISKKCICMYTYICV CVCKNSCIKRIHSMNDLFCKSV*IMLDFLRVKTP AIYKLQGWITAHSI*NSVETQELGSY*ICISIW ETELSFQRS*EVWLEKLGIILAMGAGRWSE*PS RHWF*ETELSYAEPGGQVSDPWVEAMEKEIWIQC NEALSKSEFPCNGVA*FNKN*E**I*WKKISTD *VFTWCQALC*VVHYFI*LLQQF*VCIFHYFIS GKEKCPQKVRAQTAAYSTSPCSCGGLWKRPE*HL LENYIYKGLQTDQIDHHLCGPLLTICSETKESY PKG*LTFAEEIYIHT*SELLRCA*VCKREGDLN SPS*ILLNLIISIF*ALSIHQALWKVLSCHISFN PHSKPIEYGIII*VSQKFSRVLKVLPASEQQTK TGNPG*SDT*AISSCATPSYSVPQIKRHDLYKR RFTDNSCNFNFENDSELSKIVDSNDMIVYNLASL VLCVHSNYREVI*LLTIMLNYVAAFYKLVLIQS SHNDNPAYLGIISQLCIPVCVYICMYVSIFKL* FNI*IFLLPFL*LKNYT*GNVLEFSNRGKITLGQ SVMFHNSSHFSHDLCSVECVCSM*RVFIAQFLC VSLFLKVINHQQSHILELVAFLVQAVVMRQ*WS IEHVQ*MCRWWK

Frame 2

IWCS*WAGQSGPSSPERWSDPLGCSVDACATDV QA*GGSCPGRWQQ*QQQPPAPSWPRPSLQPLRP WTLPQLHWRTPPHAPNSASGHVSARHPHPAARW GSASSQMAVSAVRCALSSLGTTARRLPSVTPTGA STVTTAGTARGTQ*ECVHAGKKCLAVYQPEASM NFTLAGCISTRSYQPKYCGVCMDNRCCIPYKSK TIDVSFQCPDGLGFSRQVLWINACFCNLSCRNPN DIFADLESYPDFSEIAN*AGTNLGSWGLTQCL* SSQPLWPITFHQ*ALVTLIWTLGLHFCL*PFK* RLMVLLRPML*VFSLISFSIYSKEKCLSLAVLDY TQA*SSLSKSLEVLLDLA*IPRNGIR*TFNITN FFFRCQTTRLFGSIQMNRWNLEQ*NNLLFGACQ EVL*WVILTSAHQNYPDSKYVCTSRSSNICQVS* IVA*F*FLMESCIH*PGHC*G*VSLHPYTVKGT D*VCPSQK*NLINIPVDGKSPQLILQRQGKVSP FQKDQLTLTLNQLLTGRALGSWPGSSLNLLPCPA WGS*ELVRPLDWPVWPLRVVPWNTPLLLQSLER PSCRPCQTH*NDQDRFR*GCGSNQEVGALGSSL G*PLELEAVGLQGPPCSGHIYCRDSFHSLSFC*P NGQFSGRKMEVYRLFRNRNRLNKGLKLKRLKLK GKGCC**ISGYYLLY*ENIIFTVRILLFRAFSV PDIALSALHVLAH*IFTTMLRSSHYYFCSYKCET EAHRGEKTQPESPSW*LGKLGFRSKLDCLYNPY FPPVFRASKCVRIGKHCNKWLDFLMSFFPLIVF LAPFQKTRISDFLII*VLKHPKYMGHTKIQESPV AYSLFPIGTSSHHTFKR*FCLPNAAY*MLCSYS QGILCSVQRDS*TGKGLGIHPPYCDLPKT*SSA RYTVVLIKYLMNTGSCACVPTFIRSSLLPKLHEN RILTVKILY*VP*QKRHLIFQRNAYVCIHIFVY AYARILV*REFTP*MISSVSQCESC*IF*E*KH LPSTNYKAG*QLTPFEIQWKPKS*VLTEFASQFG KLNLAFKDHRKSGWRN*GLFWQWVQEGGQNNPV AIGFEKRNYLMQSPEGKSQTHGLKPWRRKFGSN VMKRFLSQNFPAMVWPDSIKIKNNKYNGKKSPLI ECLLGAKHYAKLFIILFNCYSNFEYASFTIL*V EKRSAPKKLELKQQLILPAPALAEASGKDLNDT YWRITSTRGFKQTK*IITSVVPC*LYVLRQRKAT LRVS*PLLRKFTFILRVNYSGVLRCAKGKET*I HQVKSC*TLS*AFFERLAYTKPCGRCFPAISHL ILTANL*NMALSSESHRSLVVYSRSYQLVNSRPR LETQDSLIPEPSLLVLRLVILSPKSKGMTFIRG ALLTIAAILTLKMIQNYQR**IRMT*LSIISLA LYCVCIAITGK*SSS*LLC*TMSLLFTNLS*SKA VTMITLHIWES*VNYVSLCVYIYVCMYLFSNCD LIFKYSYCHFCD*KTTHEETS*NFPIEEK*HLG NLSCFTTVLIFLMICVAWNVFAQCEGFSLLNFSV *VFSLR**TISKVTYWSWWLFLYRQLL*DNDGA LSMFNKCADGG

Sequence WISP1.bAug05

ATATCTGGTGCTCCTGATGGGCCGGCCAGTCTGGGCCCAGCTCCCCCGAGAGGTGGTCGGATCCTCTGGGCTGCTCGGTCGATGCCTGTGCCACTGACGT CCAGGCATGAGGTGGTTCCTGCCCTGGACGCTGGCAGCAGTGACAGCAGCAGCCGCCAGCACCGTCCTGGCCACGGCCCTCTCTCCAGCCCCTACGACCA TGGACTTTACCCCAGCTCCACTGGAGGACACCTCCTCACGCCCCCAATTCTGCAAGTGGCCATGTGAGTGCCCGCCATCCCCACCCCGCTGCCCGCTGGG GGTCAGCCTCATCACAGATGGCTGTGAGTGCTGTAAGATGTGCGCTCAGCAGCTTGGGGACAACTGCACGGAGGCTGCCATCTGTGACCCCCACCGGGGC CTCTACTGTGACTACAGCGGGGACCGCCCGAGGTACGCAATAGGAGTGTGTGCACATGCTGTGGGTGAGGTGGAGGCATGGCACAGGAACTGCATAGCCT ACACAAGCCCCTGGAGCCCTTGCTCCACCAGCTGCGGCCTGGGGGTCTCCACTCGGATCTCCAATGTTAACGCCCAGTGCTGGCCTGAGCAAGAGAGCCG CCTCTGCAACTTGCGGCCATGCGATGTGGACATCCATACACTCATTAAGGCAGGGAAGAAGTGTCTGGCTGTGTACCAGCCAGAGGCATCCATGAACTTC ACACTTGCGGGCTGCATCAGCACACGCTCCTATCAACCCAAGTACTGTGGAGTTTGCATGGACAATAGGTGCTGCATCCCCTACAAGTCTAAGACTATCG ACGTGTCCTTCCAGTGTCCTGATGGGCTTGGCTTCTCCCGCCAGGTCCTATGGATTAATGCCTGCTTCTGTAACCTGAGCTGTAGGAATCCCAATGACAT CTTTGCTGACTTGGAATCCTACCCTGACTTCTCAGAAATTGCCAACTAGGCAGGCACAAATCTTGGGTCTTGGGGACTAACCCAATGCCTGTGAAGCAGT CAGCCCTTATGGCCAATAACTTTTCACCAATGAGCCTTAGTTACCCTGATCTGGACCCTTGGCCTCCATTTCTGTCTCTAACCATTCAAATGACGCCTGA TGGTGCTGCTCAGGCCCATGCTATGAGTTTTCTCCTTGATATCATTCAGCATCTACTCTAAAGAAAAATGCCTGTCTCTAGCTGTTCTGGACTACACCCA AGCCTGATCCAGCCTTTCCAAGTCACTAGAAGTCCTGCTGGATCTTGCCTAAATCCCAAGAAATGGAATCAGGTAGACTTTTAATATCACTAATTTCTTC TTTAGATGCCAAACCACAAGACTCTTTGGGTCCATTCAGATGAATAGATGGAATTTGGAACAATAGAATAATCTATTATTTGGAGCCTGCCAAGAGGTAC TGTAATGGGTAATTCTGACGTCAGCGCACCAAAACTATCCTGATTCCAAATATGTATGCACCTCAAGGTCATCAAACATTTGCCAAGTGAGTTGAATAGT TGCTTAATTTTGATTTTTAATGGAAAGTTGTATCCATTAACCTGGGCATTGTTGAGGTTAAGTTTCTCTTCACCCCTACACTGTGAAGGGTACAGATTAG GTTTGTCCCAGTCAGAAATAAAATTTGATAAACATTCCTGTTGATGGGAAAAGCCCCCAGTTAATACTCCAGAGACAGGGAAAGGTCAGCCCGTTTCAGA AGGACCAATTGACTCTCACACTGAATCAGCTGCTGACTGGCAGGGCTTTGGGCAGTTGGCCAGGCTCTTCCTTGAATCTTCTCCCTTGTCCTGCTTGGGG TTCATAGGAATTGGTAAGGCCTCTGGACTGGCCTGTCTGGCCCCTGAGAGTGGTGCCCTGGAACACTCCTCTACTCTTACAGAGCCTTGAGAGACCCAGC TGCAGACCATGCCAGACCCACTGAAATGACCAAGACAGGTTCAGGTAGGGGTGTGGGTCAAACCAAGAAGTGGGTGCCCTTGGTAGCAGCCTGGGGTGAC CTCTAGAGCTGGAGGCTGTGGGACTCCAGGGGCCCCCGTGTTCAGGACACATCTATTGCAGAGACTCATTTCACAGCCTTTCGTTCTGCTGACCAAATGG CCAGTTTTCTGGTAGGAAGATGGAGGTTTACCGGTTGTTTAGAAACAGAAATAGACTTAATAAAGGTTTAAAGCTGAAGAGGTTGAAGCTAAAAGGAAAA GGTTGTTGTTAATGAATATCAGGCTATTATTTATTGTATTAGGAAAATATAATATTTACTGTTAGAATTCTTTTATTTAGGGCCTTTTCTGTGCCAGACA TTGCTCTCAGTGCTTTGCATGTATTAGCTCACTGAATCTTCACGACAATGTTGAGAAGTTCCCATTATTATTTCTGTTCTTACAAATGTGAAACGGAAGC TCATAGAGGTGAGAAAACTCAACCAGAGTCACCCAGTTGGTGACTGGGAAAGTTAGGATTCAGATCGAAATTGGACTGTCTTTATAACCCATATTTTCCC CCTGTTTTTAGAGCTTCCAAATGTGTCAGAATAGGAAAACATTGCAATAAATGGCTTGATTTTTTAATGTCATTTTTCCCTCTTATAGTCTTTCTAGCTC CTTTTCAAAAGACGAGAATATCTGATTTTCTGATAATTTAGGTGCTTAAGCATCCAAAATACATGGGACACACAAAAATCCAGGAATCCCCTGTAGCTTA TTCCCTCTTTCCCATCGGAACCAGCTCTCATCACACATTTAAAAGATGATTCTGTTTACCCAATGCTGCATATTGAATGTTGTGTAGTTATTCACAGGGA ATTCTGTGCAGTGTGCAGAGAGATTCCTAAACGGGAAAAGGACTGGGAATACATCCTCCTTACTGTGACCTCCCCAAAACCTAGTCCAGTGCAAGGTATA CAGTGGTGCTCATTAAATACTTGATGAATACAGGAAGCTGTGCATGTGTTCCTACTTTTATTCGAAGCTCTCTTCTTCCAAAGCTACATGAAAATAGAAT TTTAACAGTCAAAATTTTATATTAAGTGCCTTAGCAAAAGAGACATTTAATATTTCAAAGAAATGCATATGTATGTATACATATATTTGTGTATGCGTAT GCAAGAATTCTTGTATAAAGAGAATTCACTCCATGAATGATCTCTTCTGTAAGTCAGTGTGAATCATGTTAGATTTTCTGAGAGTGAAAACACCTGCCAT CTACAAATTACAAGGCTGGATAACAGCTCACTCCATTTGAAATTCAGTGGAAACCCAAGAGCTAGGTTCTTACTGAATTTGCATCTCAATTTGGGAAACT GAACTTAGCTTTCAAAGATCATAGGAAGTCTGGTTGGAGAAACTAGGGATTATTCTGGCAATGGGTGCAGGAAGGTGGTCAGAATAACCCAGTCGCCATT GGTTTTGAGAAACGGAACTATCTTATGCAGAGCCCGGAGGGCAAGTCTCAGACCCATGGGTTGAAGCCATGGAGAAGGAAATTTGGATCCAATGTAATGA AGCGCTTTCTAAGTCAGAATTTCCCTGCAATGGTGTGGCCTGATTCAATAAAAATTAAGAATAATAAATATAATGGAAAAAAATCTCCACTGATTGAGTG TTTACTTGGTGCCAAGCACTATGCTAAGTTGTTCATTATTTTATTTAATTGTTACAGCAATTTTGAGTATGCATCTTTCACTATTTTATAAGTGGAAAAG AGAAGTGCCCCCAAAAAGTTAGAGCTCAAACAGCAGCTTATTCTACCAGCCCCTGCTCTTGCGGAGGCCTCTGGAAAAGACCTGAATGACACCTATTGGA GAATTACATCTACAAGGGGCTTCAAACAGACCAAATAGATCATCACCTCTGTGGTCCCTTGTTAACTATATGTTCTGAGACAAAGGAAAGCTACCCTAAG GGTTAGTTAACCTTTGCTGAGGAAATTTACATTCATACTTAGAGTGAATTACTCAGGTGTGCTTAGGTGTGCAAAAGGGAAGGAGACCTGAATTCACCAA GTTAAATCTTGCTAAACCTTATCATAAGCATTTTTTGAGCGCTTAGCATACACCAAGCCTTGTGGAAGGTGCTTTCCTGCCATATCTCATTTAATCCTCA CAGCAAACCTATAGAATATGGCATTATCATCTGAGTCTCACAGAAGTTTAGTCGTGTACTCAAGGTCTTACCAGCTAGTGAACAGCAGACCAAGACTGGA AACCCAGGATAGTCTGATACCTGAGCCATCTCTTCTTGTGCTACGCCTAGTTATTCTGTCCCCCAAATCAAAAGGCATGACCTTTATAAGAGGCGCTTTA CTGACAATAGCTGCAATTTTAACTTTGAAAATGATTCAGAATTATCAAAGATAGTAGATTCGAATGACATGATTGTCTATAATCTCGCTAGCCTTGTACT GTGTGTGCATAGCAATTACAGGGAAGTAATCTAGCTCCTGACTATTATGTTGAACTATGTCGCTGCTTTTTACAAACTTGTCTTGATCCAAAGCAGTCAC AATGATAACCCTGCATATCTGGGAATCATAAGTCAACTATGTATCCCTGTGTGTGTATATATATGTATGTATGTATCTATTTTCAAACTGTGATTTAATA TTTAAATATTCCTACTGCCATTTTTGTGACTGAAAAACTACACATGAGGAAACGTCTTAGAATTTTCCAATAGAGGAAAAATAACACTTGGGCAATCTGT CATGTTTCACAACAGTTCTCATTTTTCTCATGATTTGTGTAGCGTGGAATGTGTTTGCTCAATGTGAAGGGTTTTCATTGCTCAATTTCTCTGTGTAAGT CTTTTCCTTAAGGTAATAAACCATCAGCAAAGTCACATACTGGAGTTGGTGGCTTTTCTTGTACAGGCAGTTGTTATGAGACAATGATGGAGCATTGAGC ATGTTCAATAAATGTGCAGATGGTGGAAA

WISP1.u.f.4 (forward primer)
WISP1.u.r.4 (reverse primer)
Single exon skipping

Frame 0

ISGAPDGPASLGPAPPRGGRILWAARSMPVPLT SRHEVVPALDAGSSDSSSRQHRPGHGPLSSPYDH GLYPSSTGGHLLTPPILQVAM*VPAIPTPLPAG GQPHHRWL*VL*DVRSAAWGQLHGGCHL*PPPG PLL*LQRGPPEVRNRSVCTCCG*GGGMAQELHSL HKPLEPLLHQLRPGGLHSDLQC*RPVLA*AREP PLQLAAMRCGHPYTH*GREEVSGCVPARGIHEL HTCGLHQHTLLSTQVLWSLHGQ*VLHPLQV*DYR RVLPVS*WAWLLPPGPMD*CLLL*PEL*ESQ*H LC*LGILP*LLRNCQLGRHKSWVLGTNPMPVKQ SALMANNFSPMSLSYPDLDPWPPFLSLTIQMTPD GAAQAHAMSFLLDIIQHLL*RKMPVSSCSGLHP SLIQPFQVTRSPAGSCLNPKKWNQVDF*YH*FL L*MPNHKTLWVHSDE*MEFGTIE*SIIWSLPRGT VMGNSDVSAPKLS*FQICMHLKVIKHLPSELNS CLILIFNGKLYPLTWALLRLSFSSPLHCEGYRL GLSQSEIKFDKHSC*WEKPPVNTPETGKGQPVSE GPIDSHTESAADWQGFGQLARLFLESSPLSCLG FIGIGKASGLACLAPESGALEHSSTLTEP*ETQ LQTMPDPLK*PRQVQVGVWVKPRSGCPW*QPGVT SRAGGCGTPGAPVFRTHLLQRLISQPFVLLTKW PVFW*EDGGLPVV*KQK*T**RFKAEEVEAKRK RLLLMNIRLLFIVLGKYNIYC*NSFI*GLFCARH CSQCFACISSLNLHDNVEKFPLLFLFLQM*NGS S*R*ENSTRVTQLVTGKVRIQIEIGLSL*PIFS PCF*SFQMCQNRKTLQ*MA*FFNVIFPSYSLSSS FSKDENI*FSDNLGA*ASKIHGTHKNPGIPCSL FPLSHRNQLSSHI*KMILFTQCCILNVV*LFTG NSVQCAERFLNGKRTGNTSSLL*PPQNLVQCKVY SGAH*ILDEYRKLCMCSYFYSKLSSSKAT*K*N FNSQNFILSALAKETFNISKKCICMYTYICVCV CKNSCIKRIHSMNDLFCKSV*IMLDFLRVKTPAI YKLQGWITAHSI*NSVETQELGSY*ICISIWET ELSFQRS*EVWLEKLGIILAMGAGRWSE*PSRH WF*ETELSYAEPGGQVSDPWVEAMEKEIWIQCNE ALSKSEFPCNGVA*FNKN*E**I*WKKISTD*V FTWCQALC*VVHYFI*LLQQF*VCIFHYFISGK EKCPQKVRAQTAAYSTSPCSCGGLWKRPE*HLLE NYIYKGLQTDQIDHHLCGPLLTICSETKESYPK G*LTFAEEIYIHT*SELLRCA*VCKREGDLNSP S*ILLNLIISIF*ALSIHQALWKVLSCHISFNPH SKPIEYGIII*VSQKFSRVLKVLPASEQQTKTG NPG*SDT*AISSCATPSYSVPQIKRHDLYKRRF TDNSCNFNFENDSELSKIVDSNDMIVYNLASLVL CVHSNYREVI*LLTIMLNYVAAFYKLVLIQSSH NDNPAYLGIISQLCIPVCVYICMYVSIFKL*FN I*IFLLPFL*LKNYT*GNVLEFSNRGKITLGQSV MFHNSSHFSHDLCSVECVCSM*RVFIAQFLCVS LFLKVINHQQSHILELVAFLVQAVVMRQ*WSIE HVQ*MCRWWK

Frame 1

YLVLLMGRPVWAQLPREVVGSSGLLGRCLCH*R PGMRWFLPWTLAAVTAAAASTVLATALSPAPTT MDFTPAPLEDTSSRPQFCKWPCECPPSPPRCPLG VSLITDGCECCKMCAQQLGDNCTEAAICDPHRG LYCDYSGDRPRYAIGVCAHAVGEVEAWHRNCIA YTSPWSPCSTSCGLGVSTRISNVNAQCWPEQESR LCNLRPCDVDIHTLIKAGKKCLAVYQPEASMNF TLAGCISTRSYQPKYCGVCMDNRCCIPYKSKTI DVSFQCPDGLGFSRQVLWINACFCNLSCRNPNDI FADLESYPDFSEIAN*AGTNLGSWGLTQCL*SS QPLWPITFHQ*ALVTLIWTLGLHFCL*PFK*RL MVLLRPML*VFSLISFSIYSKEKCLSLAVLDYTQ A*SSLSKSLEVLLDLA*IPRNGIR*TFNITNFF FRCQTTRLFGSIQMNRWNLEQ*NNLLFGACQEV L*WVILTSAHQNYPDSKYVCTSRSSNICQVS*IV A*F*FLMESCIH*PGHC*G*VSLHPYTVKGTD* VCPSQK*NLINIPVDGKSPQLILQRQGKVSPFQ KDQLTLTLNQLLTGRALGSWPGSSLNLLPCPAWG S*ELVRPLDWPVWPLRVVPWNTPLLLQSLERPS CRPCQTH*NDQDRFR*GCGSNQEVGALGSSLG* PLELEAVGLQGPPCSGHIYCRDSFHSLSFC*PNG QFSGRKMEVYRLFRNRNRLNKGLKLKRLKLKGK GCC**ISGYYLLY*ENIIFTVRILLFRAFSVPD IALSALHVLAH*IFTTMLRSSHYYFCSYKCETEA HRGEKTQPESPSW*LGKLGFRSKLDCLYNPYFP PVFRASKCVRIGKHCNKWLDFLMSFFPLIVFLA PFQKTRISDFLII*VLKHPKYMGHTKIQESPVAY SLFPIGTSSHHTFKR*FCLPNAAY*MLCSYSQG ILCSVQRDS*TGKGLGIHPPYCDLPKT*SSARY TVVLIKYLMNTGSCACVPTFIRSSLLPKLHENRI LTVKILY*VP*QKRHLIFQRNAYVCIHIFVYAY ARILV*REFTP*MISSVSQCESC*IF*E*KHLP STNYKAG*QLTPFEIQWKPKS*VLTEFASQFGKL NLAFKDHRKSGWRN*GLFWQWVQEGGQNNPVAI GFEKRNYLMQSPEGKSQTHGLKPWRRKFGSNVM KRFLSQNFPAMVWPDSIKIKNNKYNGKKSPLIEC LLGAKHYAKLFIILFNCYSNFEYASFTIL*VEK RSAPKKLELKQQLILPAPALAEASGKDLNDTYW RITSTRGFKQTK*IITSVVPC*LYVLRQRKATLR VS*PLLRKFTFILRVNYSGVLRCAKGKET*IHQ VKSC*TLS*AFFERLAYTKPCGRCFPAISHLIL TANL*NMALSSESHRSLVVYSRSYQLVNSRPRLE TQDSLIPEPSLLVLRLVILSPKSKGMTFIRGAL LTIAAILTLKMIQNYQR**IRMT*LSIISLALY CVCIAITGK*SSS*LLC*TMSLLFTNLS*SKAVT MITLHIWES*VNYVSLCVYIYVCMYLFSNCDLI FKYSYCHFCD*KTTHEETS*NFPIEEK*HLGNL SCFTTVLIFLMICVAWNVFAQCEGFSLLNFSV*V FSLR**TISKVTYWSWWLFLYRQLL*DNDGALS MFNKCADGG

Frame 2

IWCS*WAGQSGPSSPERWSDPLGCSVDACATDV QA*GGSCPGRWQQ*QQQPPAPSWPRPSLQPLRP WTLPQLHWRTPPHAPNSASGHVSARHPHPAARW GSASSQMAVSAVRCALSSLGTTARRLPSVTPTGA STVTTAGTARGTQ*ECVHMLWVRWRHGTGTA*P TQAPGALAPPAAAWGSPLGSPMLTPSAGLSKRA ASATCGHAMWTSIHSLRQGRSVWLCTSQRHP*TS HLRAASAHAPINPSTVEFAWTIGAASPTSLRLS TCPSSVLMGLASPARSYGLMPASVT*AVGIPMT SLLTWNPTLTSQKLPTRQAQILGLGD*PNACEAV SPYGQ*LFTNEP*LP*SGPLASISVSNHSNDA* WCCSGPCYEFSP*YHSASTLKKNACL*LFWTTP KPDPAFPSH*KSCWILPKSQEMESGRLLISLISS LDAKPQDSLGPFR*IDGIWNNRIIYYLEPAKRY CNG*F*RQRTKTILIPNMYAPQGHQTFAK*VE* LLNFDF*WKVVSINLGIVEVKFLFTPTL*RVQIR FVPVRNKI**TFLLMGKAPS*YSRDRERSARFR RTN*LSH*ISC*LAGLWAVGQALP*IFSLVLLG VHRNW*GLWTGLSGP*EWCPGTLLYSYRALRDPA ADHARPTEMTKTGSGRGVGQTKKWVPLVAAWGD L*SWRLWDSRGPRVQDTSIAETHFTAFRSADQM ASFLVGRWRFTGCLETEIDLIKV*S*RG*S*KEK VVVNEYQAIIYCIRKI*YLLLEFFYLGPFLCQT LLSVLCMY*LTESSRQC*EVPIIISVLTNVKRK LIEVRKLNQSHPVGDWES*DSDRNWTVFITHIFP LFLELPNVSE*ENIAINGLIF*CHFSLL*SF*L LFKRREYLIF**FRCLSIQNTWDTQKSRNPL*L IPSFPSEPALITHLKDDSVYPMLHIECCVVIHRE FCAVCREIPKREKDWEYILLTVTSPKPSPVQGI QWCSLNT**IQEAVHVFLLLFEALFFQSYMKIE F*QSKFYIKCLSKRDI*YFKEMHMYVYIYLCMRM QEFLYKENSLHE*SLL*VSVNHVRFSESENTCH LQITRLDNSSLHLKFSGNPRARFLLNLHLNLGN *T*LSKIIGSLVGETRDYSGNGCRKVVRITQSPL VLRNGTILCRARRASLRPMG*SHGEGNLDPM** SAF*VRISLQWCGLIQ*KLRIINIMEKNLH*LS VYLVPSTMLSCSLFYLIVTAILSMHLSLFYKWKR EVPPKS*SSNSSLFYQPLLLRRPLEKT*MTPIG ELHLQGASNRPNRSSPLWSLVNYMF*DKGKLP* GLVNLC*GNLHSYLE*ITQVCLGVQKGRRPEFTK LNLAKPYHKHFLSA*HTPSLVEGAFLPYLI*SS QQTYRIWHYHLSLTEV*SCTQGLTS**TADQDW KPRIV*YLSHLFLCYA*LFCPPNQKA*PL*EALY *Q*LQF*L*K*FRIIKDSRFE*HDCL*SR*PCT VCA*QLQGSNLAPDYYVELCRCFLQTCLDPKQS Q**PCISGNHKSTMYPCVCIYMYVCIYFQTVI*Y LNIPTAIFVTEKLHMRKRLRIFQ*RKNNTWAIC HVSQQFSFFS*FV*RGMCLLNVKGFHCSISLCK SFP*GNKPSAKSHTGVGGFSCTGSCYETMMEH*A CSINVQMVE