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Sequences

Sequence WISP1.eAug05

ATATCTGGTGCTCCTGATGGGCCGGCCAGTCTGGGCCCAGCTCCCCCGAGAGGTGGTCGGATCCTCTGGGCTGCTCGGTCGATGCCTGTGCCACTGACGT CCAGGCATGAGGTGGTTCCTGCCCTGGACGCTGGCAGCAGTGACAGCAGCAGCCGCCAGCACCGTCCTGGCCACGGCAGGGAAGAAGTGTCTGGCTGTGT ACCAGCCAGAGGCATCCATGAACTTCACACTTGCGGGCTGCATCAGCACACGCTCCTATCAACCCAAGTACTGTGGAGTTTGCATGGACAATAGGTGCTG CATCCCCTACAAGTCTAAGACTATCGACGTGTCCTTCCAGTGTCCTGATGGGCTTGGCTTCTCCCGCCAGGTCCTATGGATTAATGCCTGCTTCTGTAAC CTGAGCTGTAGGAATCCCAATGACATCTTTGCTGACTTGGAATCCTACCCTGACTTCTCAGAAATTGCCAACTAGGCAGGCACAAATCTTGGGTCTTGGG GACTAACCCAATGCCTGTGAAGCAGTCAGCCCTTATGGCCAATAACTTTTCACCAATGAGCCTTAGTTACCCTGATCTGGACCCTTGGCCTCCATTTCTG TCTCTAACCATTCAAATGACGCCTGATGGTGCTGCTCAGGCCCATGCTATGAGTTTTCTCCTTGATATCATTCAGCATCTACTCTAAAGAAAAATGCCTG TCTCTAGCTGTTCTGGACTACACCCAAGCCTGATCCAGCCTTTCCAAGTCACTAGAAGTCCTGCTGGATCTTGCCTAAATCCCAAGAAATGGAATCAGGT AGACTTTTAATATCACTAATTTCTTCTTTAGATGCCAAACCACAAGACTCTTTGGGTCCATTCAGATGAATAGATGGAATTTGGAACAATAGAATAATCT ATTATTTGGAGCCTGCCAAGAGGTACTGTAATGGGTAATTCTGACGTCAGCGCACCAAAACTATCCTGATTCCAAATATGTATGCACCTCAAGGTCATCA AACATTTGCCAAGTGAGTTGAATAGTTGCTTAATTTTGATTTTTAATGGAAAGTTGTATCCATTAACCTGGGCATTGTTGAGGTTAAGTTTCTCTTCACC CCTACACTGTGAAGGGTACAGATTAGGTTTGTCCCAGTCAGAAATAAAATTTGATAAACATTCCTGTTGATGGGAAAAGCCCCCAGTTAATACTCCAGAG ACAGGGAAAGGTCAGCCCGTTTCAGAAGGACCAATTGACTCTCACACTGAATCAGCTGCTGACTGGCAGGGCTTTGGGCAGTTGGCCAGGCTCTTCCTTG AATCTTCTCCCTTGTCCTGCTTGGGGTTCATAGGAATTGGTAAGGCCTCTGGACTGGCCTGTCTGGCCCCTGAGAGTGGTGCCCTGGAACACTCCTCTAC TCTTACAGAGCCTTGAGAGACCCAGCTGCAGACCATGCCAGACCCACTGAAATGACCAAGACAGGTTCAGGTAGGGGTGTGGGTCAAACCAAGAAGTGGG TGCCCTTGGTAGCAGCCTGGGGTGACCTCTAGAGCTGGAGGCTGTGGGACTCCAGGGGCCCCCGTGTTCAGGACACATCTATTGCAGAGACTCATTTCAC AGCCTTTCGTTCTGCTGACCAAATGGCCAGTTTTCTGGTAGGAAGATGGAGGTTTACCGGTTGTTTAGAAACAGAAATAGACTTAATAAAGGTTTAAAGC TGAAGAGGTTGAAGCTAAAAGGAAAAGGTTGTTGTTAATGAATATCAGGCTATTATTTATTGTATTAGGAAAATATAATATTTACTGTTAGAATTCTTTT ATTTAGGGCCTTTTCTGTGCCAGACATTGCTCTCAGTGCTTTGCATGTATTAGCTCACTGAATCTTCACGACAATGTTGAGAAGTTCCCATTATTATTTC TGTTCTTACAAATGTGAAACGGAAGCTCATAGAGGTGAGAAAACTCAACCAGAGTCACCCAGTTGGTGACTGGGAAAGTTAGGATTCAGATCGAAATTGG ACTGTCTTTATAACCCATATTTTCCCCCTGTTTTTAGAGCTTCCAAATGTGTCAGAATAGGAAAACATTGCAATAAATGGCTTGATTTTTTAATGTCATT TTTCCCTCTTATAGTCTTTCTAGCTCCTTTTCAAAAGACGAGAATATCTGATTTTCTGATAATTTAGGTGCTTAAGCATCCAAAATACATGGGACACACA AAAATCCAGGAATCCCCTGTAGCTTATTCCCTCTTTCCCATCGGAACCAGCTCTCATCACACATTTAAAAGATGATTCTGTTTACCCAATGCTGCATATT GAATGTTGTGTAGTTATTCACAGGGAATTCTGTGCAGTGTGCAGAGAGATTCCTAAACGGGAAAAGGACTGGGAATACATCCTCCTTACTGTGACCTCCC CAAAACCTAGTCCAGTGCAAGGTATACAGTGGTGCTCATTAAATACTTGATGAATACAGGAAGCTGTGCATGTGTTCCTACTTTTATTCGAAGCTCTCTT CTTCCAAAGCTACATGAAAATAGAATTTTAACAGTCAAAATTTTATATTAAGTGCCTTAGCAAAAGAGACATTTAATATTTCAAAGAAATGCATATGTAT GTATACATATATTTGTGTATGCGTATGCAAGAATTCTTGTATAAAGAGAATTCACTCCATGAATGATCTCTTCTGTAAGTCAGTGTGAATCATGTTAGAT TTTCTGAGAGTGAAAACACCTGCCATCTACAAATTACAAGGCTGGATAACAGCTCACTCCATTTGAAATTCAGTGGAAACCCAAGAGCTAGGTTCTTACT GAATTTGCATCTCAATTTGGGAAACTGAACTTAGCTTTCAAAGATCATAGGAAGTCTGGTTGGAGAAACTAGGGATTATTCTGGCAATGGGTGCAGGAAG GTGGTCAGAATAACCCAGTCGCCATTGGTTTTGAGAAACGGAACTATCTTATGCAGAGCCCGGAGGGCAAGTCTCAGACCCATGGGTTGAAGCCATGGAG AAGGAAATTTGGATCCAATGTAATGAAGCGCTTTCTAAGTCAGAATTTCCCTGCAATGGTGTGGCCTGATTCAATAAAAATTAAGAATAATAAATATAAT GGAAAAAAATCTCCACTGATTGAGTGTTTACTTGGTGCCAAGCACTATGCTAAGTTGTTCATTATTTTATTTAATTGTTACAGCAATTTTGAGTATGCAT CTTTCACTATTTTATAAGTGGAAAAGAGAAGTGCCCCCAAAAAGTTAGAGCTCAAACAGCAGCTTATTCTACCAGCCCCTGCTCTTGCGGAGGCCTCTGG AAAAGACCTGAATGACACCTATTGGAGAATTACATCTACAAGGGGCTTCAAACAGACCAAATAGATCATCACCTCTGTGGTCCCTTGTTAACTATATGTT CTGAGACAAAGGAAAGCTACCCTAAGGGTTAGTTAACCTTTGCTGAGGAAATTTACATTCATACTTAGAGTGAATTACTCAGGTGTGCTTAGGTGTGCAA AAGGGAAGGAGACCTGAATTCACCAAGTTAAATCTTGCTAAACCTTATCATAAGCATTTTTTGAGCGCTTAGCATACACCAAGCCTTGTGGAAGGTGCTT TCCTGCCATATCTCATTTAATCCTCACAGCAAACCTATAGAATATGGCATTATCATCTGAGTCTCACAGAAGTTTAGTCGTGTACTCAAGGTCTTACCAG CTAGTGAACAGCAGACCAAGACTGGAAACCCAGGATAGTCTGATACCTGAGCCATCTCTTCTTGTGCTACGCCTAGTTATTCTGTCCCCCAAATCAAAAG GCATGACCTTTATAAGAGGCGCTTTACTGACAATAGCTGCAATTTTAACTTTGAAAATGATTCAGAATTATCAAAGATAGTAGATTCGAATGACATGATT GTCTATAATCTCGCTAGCCTTGTACTGTGTGTGCATAGCAATTACAGGGAAGTAATCTAGCTCCTGACTATTATGTTGAACTATGTCGCTGCTTTTTACA AACTTGTCTTGATCCAAAGCAGTCACAATGATAACCCTGCATATCTGGGAATCATAAGTCAACTATGTATCCCTGTGTGTGTATATATATGTATGTATGT ATCTATTTTCAAACTGTGATTTAATATTTAAATATTCCTACTGCCATTTTTGTGACTGAAAAACTACACATGAGGAAACGTCTTAGAATTTTCCAATAGA GGAAAAATAACACTTGGGCAATCTGTCATGTTTCACAACAGTTCTCATTTTTCTCATGATTTGTGTAGCGTGGAATGTGTTTGCTCAATGTGAAGGGTTT TCATTGCTCAATTTCTCTGTGTAAGTCTTTTCCTTAAGGTAATAAACCATCAGCAAAGTCACATACTGGAGTTGGTGGCTTTTCTTGTACAGGCAGTTGT TATGAGACAATGATGGAGCATTGAGCATGTTCAATAAATGTGCAGATGGTGGAAA

WISP1.u.f.1 (forward primer)
WISP1.u.r.4 (reverse primer)
Single exon skipping

Frame 0

ISGAPDGPASLGPAPPRGGRILWAARSMPVPLT SRHEVVPALDAGSSDSSSRQHRPGHGREEVSGCV PARGIHELHTCGLHQHTLLSTQVLWSLHGQ*VL HPLQV*DYRRVLPVS*WAWLLPPGPMD*CLLL* PEL*ESQ*HLC*LGILP*LLRNCQLGRHKSWVLG TNPMPVKQSALMANNFSPMSLSYPDLDPWPPFL SLTIQMTPDGAAQAHAMSFLLDIIQHLL*RKMP VSSCSGLHPSLIQPFQVTRSPAGSCLNPKKWNQV DF*YH*FLL*MPNHKTLWVHSDE*MEFGTIE*S IIWSLPRGTVMGNSDVSAPKLS*FQICMHLKVI KHLPSELNSCLILIFNGKLYPLTWALLRLSFSSP LHCEGYRLGLSQSEIKFDKHSC*WEKPPVNTPE TGKGQPVSEGPIDSHTESAADWQGFGQLARLFL ESSPLSCLGFIGIGKASGLACLAPESGALEHSST LTEP*ETQLQTMPDPLK*PRQVQVGVWVKPRSG CPW*QPGVTSRAGGCGTPGAPVFRTHLLQRLIS QPFVLLTKWPVFW*EDGGLPVV*KQK*T**RFKA EEVEAKRKRLLLMNIRLLFIVLGKYNIYC*NSF I*GLFCARHCSQCFACISSLNLHDNVEKFPLLF LFLQM*NGSS*R*ENSTRVTQLVTGKVRIQIEIG LSL*PIFSPCF*SFQMCQNRKTLQ*MA*FFNVI FPSYSLSSSFSKDENI*FSDNLGA*ASKIHGTH KNPGIPCSLFPLSHRNQLSSHI*KMILFTQCCIL NVV*LFTGNSVQCAERFLNGKRTGNTSSLL*PP QNLVQCKVYSGAH*ILDEYRKLCMCSYFYSKLS SSKAT*K*NFNSQNFILSALAKETFNISKKCICM YTYICVCVCKNSCIKRIHSMNDLFCKSV*IMLD FLRVKTPAIYKLQGWITAHSI*NSVETQELGSY *ICISIWETELSFQRS*EVWLEKLGIILAMGAGR WSE*PSRHWF*ETELSYAEPGGQVSDPWVEAME KEIWIQCNEALSKSEFPCNGVA*FNKN*E**I* WKKISTD*VFTWCQALC*VVHYFI*LLQQF*VCI FHYFISGKEKCPQKVRAQTAAYSTSPCSCGGLW KRPE*HLLENYIYKGLQTDQIDHHLCGPLLTIC SETKESYPKG*LTFAEEIYIHT*SELLRCA*VCK REGDLNSPS*ILLNLIISIF*ALSIHQALWKVL SCHISFNPHSKPIEYGIII*VSQKFSRVLKVLP ASEQQTKTGNPG*SDT*AISSCATPSYSVPQIKR HDLYKRRFTDNSCNFNFENDSELSKIVDSNDMI VYNLASLVLCVHSNYREVI*LLTIMLNYVAAFY KLVLIQSSHNDNPAYLGIISQLCIPVCVYICMYV SIFKL*FNI*IFLLPFL*LKNYT*GNVLEFSNR GKITLGQSVMFHNSSHFSHDLCSVECVCSM*RV FIAQFLCVSLFLKVINHQQSHILELVAFLVQAVV MRQ*WSIEHVQ*MCRWWK

Frame 1

YLVLLMGRPVWAQLPREVVGSSGLLGRCLCH*R PGMRWFLPWTLAAVTAAAASTVLATAGKKCLAV YQPEASMNFTLAGCISTRSYQPKYCGVCMDNRCC IPYKSKTIDVSFQCPDGLGFSRQVLWINACFCN LSCRNPNDIFADLESYPDFSEIAN*AGTNLGSW GLTQCL*SSQPLWPITFHQ*ALVTLIWTLGLHFC L*PFK*RLMVLLRPML*VFSLISFSIYSKEKCL SLAVLDYTQA*SSLSKSLEVLLDLA*IPRNGIR *TFNITNFFFRCQTTRLFGSIQMNRWNLEQ*NNL LFGACQEVL*WVILTSAHQNYPDSKYVCTSRSS NICQVS*IVA*F*FLMESCIH*PGHC*G*VSLH PYTVKGTD*VCPSQK*NLINIPVDGKSPQLILQR QGKVSPFQKDQLTLTLNQLLTGRALGSWPGSSL NLLPCPAWGS*ELVRPLDWPVWPLRVVPWNTPL LLQSLERPSCRPCQTH*NDQDRFR*GCGSNQEVG ALGSSLG*PLELEAVGLQGPPCSGHIYCRDSFH SLSFC*PNGQFSGRKMEVYRLFRNRNRLNKGLK LKRLKLKGKGCC**ISGYYLLY*ENIIFTVRILL FRAFSVPDIALSALHVLAH*IFTTMLRSSHYYF CSYKCETEAHRGEKTQPESPSW*LGKLGFRSKL DCLYNPYFPPVFRASKCVRIGKHCNKWLDFLMSF FPLIVFLAPFQKTRISDFLII*VLKHPKYMGHT KIQESPVAYSLFPIGTSSHHTFKR*FCLPNAAY *MLCSYSQGILCSVQRDS*TGKGLGIHPPYCDLP KT*SSARYTVVLIKYLMNTGSCACVPTFIRSSL LPKLHENRILTVKILY*VP*QKRHLIFQRNAYV CIHIFVYAYARILV*REFTP*MISSVSQCESC*I F*E*KHLPSTNYKAG*QLTPFEIQWKPKS*VLT EFASQFGKLNLAFKDHRKSGWRN*GLFWQWVQE GGQNNPVAIGFEKRNYLMQSPEGKSQTHGLKPWR RKFGSNVMKRFLSQNFPAMVWPDSIKIKNNKYN GKKSPLIECLLGAKHYAKLFIILFNCYSNFEYA SFTIL*VEKRSAPKKLELKQQLILPAPALAEASG KDLNDTYWRITSTRGFKQTK*IITSVVPC*LYV LRQRKATLRVS*PLLRKFTFILRVNYSGVLRCA KGKET*IHQVKSC*TLS*AFFERLAYTKPCGRCF PAISHLILTANL*NMALSSESHRSLVVYSRSYQ LVNSRPRLETQDSLIPEPSLLVLRLVILSPKSK GMTFIRGALLTIAAILTLKMIQNYQR**IRMT*L SIISLALYCVCIAITGK*SSS*LLC*TMSLLFT NLS*SKAVTMITLHIWES*VNYVSLCVYIYVCM YLFSNCDLIFKYSYCHFCD*KTTHEETS*NFPIE EK*HLGNLSCFTTVLIFLMICVAWNVFAQCEGF SLLNFSV*VFSLR**TISKVTYWSWWLFLYRQL L*DNDGALSMFNKCADGG

Frame 2

IWCS*WAGQSGPSSPERWSDPLGCSVDACATDV QA*GGSCPGRWQQ*QQQPPAPSWPRQGRSVWLC TSQRHP*TSHLRAASAHAPINPSTVEFAWTIGA ASPTSLRLSTCPSSVLMGLASPARSYGLMPASVT *AVGIPMTSLLTWNPTLTSQKLPTRQAQILGLG D*PNACEAVSPYGQ*LFTNEP*LP*SGPLASIS VSNHSNDA*WCCSGPCYEFSP*YHSASTLKKNAC L*LFWTTPKPDPAFPSH*KSCWILPKSQEMESG RLLISLISSLDAKPQDSLGPFR*IDGIWNNRII YYLEPAKRYCNG*F*RQRTKTILIPNMYAPQGHQ TFAK*VE*LLNFDF*WKVVSINLGIVEVKFLFT PTL*RVQIRFVPVRNKI**TFLLMGKAPS*YSR DRERSARFRRTN*LSH*ISC*LAGLWAVGQALP* IFSLVLLGVHRNW*GLWTGLSGP*EWCPGTLLY SYRALRDPAADHARPTEMTKTGSGRGVGQTKKW VPLVAAWGDL*SWRLWDSRGPRVQDTSIAETHFT AFRSADQMASFLVGRWRFTGCLETEIDLIKV*S *RG*S*KEKVVVNEYQAIIYCIRKI*YLLLEFF YLGPFLCQTLLSVLCMY*LTESSRQC*EVPIIIS VLTNVKRKLIEVRKLNQSHPVGDWES*DSDRNW TVFITHIFPLFLELPNVSE*ENIAINGLIF*CH FSLL*SF*LLFKRREYLIF**FRCLSIQNTWDTQ KSRNPL*LIPSFPSEPALITHLKDDSVYPMLHI ECCVVIHREFCAVCREIPKREKDWEYILLTVTS PKPSPVQGIQWCSLNT**IQEAVHVFLLLFEALF FQSYMKIEF*QSKFYIKCLSKRDI*YFKEMHMY VYIYLCMRMQEFLYKENSLHE*SLL*VSVNHVR FSESENTCHLQITRLDNSSLHLKFSGNPRARFLL NLHLNLGN*T*LSKIIGSLVGETRDYSGNGCRK VVRITQSPLVLRNGTILCRARRASLRPMG*SHG EGNLDPM**SAF*VRISLQWCGLIQ*KLRIINIM EKNLH*LSVYLVPSTMLSCSLFYLIVTAILSMH LSLFYKWKREVPPKS*SSNSSLFYQPLLLRRPL EKT*MTPIGELHLQGASNRPNRSSPLWSLVNYMF *DKGKLP*GLVNLC*GNLHSYLE*ITQVCLGVQ KGRRPEFTKLNLAKPYHKHFLSA*HTPSLVEGA FLPYLI*SSQQTYRIWHYHLSLTEV*SCTQGLTS **TADQDWKPRIV*YLSHLFLCYA*LFCPPNQK A*PL*EALY*Q*LQF*L*K*FRIIKDSRFE*HD CL*SR*PCTVCA*QLQGSNLAPDYYVELCRCFLQ TCLDPKQSQ**PCISGNHKSTMYPCVCIYMYVC IYFQTVI*YLNIPTAIFVTEKLHMRKRLRIFQ* RKNNTWAICHVSQQFSFFS*FV*RGMCLLNVKGF HCSISLCKSFP*GNKPSAKSHTGVGGFSCTGSC YETMMEH*ACSINVQMVE

Sequence WISP1.dAug05

ATATCTGGTGCTCCTGATGGGCCGGCCAGTCTGGGCCCAGCTCCCCCGAGAGGTGGTCGGATCCTCTGGGCTGCTCGGTCGATGCCTGTGCCACTGACGT CCAGGCATGAGGTGGTTCCTGCCCTGGACGCTGGCAGCAGTGACAGCAGCAGCCGCCAGCACCGTCCTGGCCACGGCCCTCTCTCCAGCCCCTACGACCA TGGACTTTACCCCAGCTCCACTGGAGGACACCTCCTCACGCCCCCAATTCTGCAAGTGGCCATGTGAGTGCCCGCCATCCCCACCCCGCTGCCCGCTGGG GGTCAGCCTCATCACAGATGGCTGTGAGTGCTGTAAGATGTGCGCTCAGCAGCTTGGGGACAACTGCACGGAGGCTGCCATCTGTGACCCCCACCGGGGC CTCTACTGTGACTACAGCGGGGACCGCCCGAGGTACGCAATAGGAGTGTGTGCACGCAGGGAAGAAGTGTCTGGCTGTGTACCAGCCAGAGGCATCCATG AACTTCACACTTGCGGGCTGCATCAGCACACGCTCCTATCAACCCAAGTACTGTGGAGTTTGCATGGACAATAGGTGCTGCATCCCCTACAAGTCTAAGA CTATCGACGTGTCCTTCCAGTGTCCTGATGGGCTTGGCTTCTCCCGCCAGGTCCTATGGATTAATGCCTGCTTCTGTAACCTGAGCTGTAGGAATCCCAA TGACATCTTTGCTGACTTGGAATCCTACCCTGACTTCTCAGAAATTGCCAACTAGGCAGGCACAAATCTTGGGTCTTGGGGACTAACCCAATGCCTGTGA AGCAGTCAGCCCTTATGGCCAATAACTTTTCACCAATGAGCCTTAGTTACCCTGATCTGGACCCTTGGCCTCCATTTCTGTCTCTAACCATTCAAATGAC GCCTGATGGTGCTGCTCAGGCCCATGCTATGAGTTTTCTCCTTGATATCATTCAGCATCTACTCTAAAGAAAAATGCCTGTCTCTAGCTGTTCTGGACTA CACCCAAGCCTGATCCAGCCTTTCCAAGTCACTAGAAGTCCTGCTGGATCTTGCCTAAATCCCAAGAAATGGAATCAGGTAGACTTTTAATATCACTAAT TTCTTCTTTAGATGCCAAACCACAAGACTCTTTGGGTCCATTCAGATGAATAGATGGAATTTGGAACAATAGAATAATCTATTATTTGGAGCCTGCCAAG AGGTACTGTAATGGGTAATTCTGACGTCAGCGCACCAAAACTATCCTGATTCCAAATATGTATGCACCTCAAGGTCATCAAACATTTGCCAAGTGAGTTG AATAGTTGCTTAATTTTGATTTTTAATGGAAAGTTGTATCCATTAACCTGGGCATTGTTGAGGTTAAGTTTCTCTTCACCCCTACACTGTGAAGGGTACA GATTAGGTTTGTCCCAGTCAGAAATAAAATTTGATAAACATTCCTGTTGATGGGAAAAGCCCCCAGTTAATACTCCAGAGACAGGGAAAGGTCAGCCCGT TTCAGAAGGACCAATTGACTCTCACACTGAATCAGCTGCTGACTGGCAGGGCTTTGGGCAGTTGGCCAGGCTCTTCCTTGAATCTTCTCCCTTGTCCTGC TTGGGGTTCATAGGAATTGGTAAGGCCTCTGGACTGGCCTGTCTGGCCCCTGAGAGTGGTGCCCTGGAACACTCCTCTACTCTTACAGAGCCTTGAGAGA CCCAGCTGCAGACCATGCCAGACCCACTGAAATGACCAAGACAGGTTCAGGTAGGGGTGTGGGTCAAACCAAGAAGTGGGTGCCCTTGGTAGCAGCCTGG GGTGACCTCTAGAGCTGGAGGCTGTGGGACTCCAGGGGCCCCCGTGTTCAGGACACATCTATTGCAGAGACTCATTTCACAGCCTTTCGTTCTGCTGACC AAATGGCCAGTTTTCTGGTAGGAAGATGGAGGTTTACCGGTTGTTTAGAAACAGAAATAGACTTAATAAAGGTTTAAAGCTGAAGAGGTTGAAGCTAAAA GGAAAAGGTTGTTGTTAATGAATATCAGGCTATTATTTATTGTATTAGGAAAATATAATATTTACTGTTAGAATTCTTTTATTTAGGGCCTTTTCTGTGC CAGACATTGCTCTCAGTGCTTTGCATGTATTAGCTCACTGAATCTTCACGACAATGTTGAGAAGTTCCCATTATTATTTCTGTTCTTACAAATGTGAAAC GGAAGCTCATAGAGGTGAGAAAACTCAACCAGAGTCACCCAGTTGGTGACTGGGAAAGTTAGGATTCAGATCGAAATTGGACTGTCTTTATAACCCATAT TTTCCCCCTGTTTTTAGAGCTTCCAAATGTGTCAGAATAGGAAAACATTGCAATAAATGGCTTGATTTTTTAATGTCATTTTTCCCTCTTATAGTCTTTC TAGCTCCTTTTCAAAAGACGAGAATATCTGATTTTCTGATAATTTAGGTGCTTAAGCATCCAAAATACATGGGACACACAAAAATCCAGGAATCCCCTGT AGCTTATTCCCTCTTTCCCATCGGAACCAGCTCTCATCACACATTTAAAAGATGATTCTGTTTACCCAATGCTGCATATTGAATGTTGTGTAGTTATTCA CAGGGAATTCTGTGCAGTGTGCAGAGAGATTCCTAAACGGGAAAAGGACTGGGAATACATCCTCCTTACTGTGACCTCCCCAAAACCTAGTCCAGTGCAA GGTATACAGTGGTGCTCATTAAATACTTGATGAATACAGGAAGCTGTGCATGTGTTCCTACTTTTATTCGAAGCTCTCTTCTTCCAAAGCTACATGAAAA TAGAATTTTAACAGTCAAAATTTTATATTAAGTGCCTTAGCAAAAGAGACATTTAATATTTCAAAGAAATGCATATGTATGTATACATATATTTGTGTAT GCGTATGCAAGAATTCTTGTATAAAGAGAATTCACTCCATGAATGATCTCTTCTGTAAGTCAGTGTGAATCATGTTAGATTTTCTGAGAGTGAAAACACC TGCCATCTACAAATTACAAGGCTGGATAACAGCTCACTCCATTTGAAATTCAGTGGAAACCCAAGAGCTAGGTTCTTACTGAATTTGCATCTCAATTTGG GAAACTGAACTTAGCTTTCAAAGATCATAGGAAGTCTGGTTGGAGAAACTAGGGATTATTCTGGCAATGGGTGCAGGAAGGTGGTCAGAATAACCCAGTC GCCATTGGTTTTGAGAAACGGAACTATCTTATGCAGAGCCCGGAGGGCAAGTCTCAGACCCATGGGTTGAAGCCATGGAGAAGGAAATTTGGATCCAATG TAATGAAGCGCTTTCTAAGTCAGAATTTCCCTGCAATGGTGTGGCCTGATTCAATAAAAATTAAGAATAATAAATATAATGGAAAAAAATCTCCACTGAT TGAGTGTTTACTTGGTGCCAAGCACTATGCTAAGTTGTTCATTATTTTATTTAATTGTTACAGCAATTTTGAGTATGCATCTTTCACTATTTTATAAGTG GAAAAGAGAAGTGCCCCCAAAAAGTTAGAGCTCAAACAGCAGCTTATTCTACCAGCCCCTGCTCTTGCGGAGGCCTCTGGAAAAGACCTGAATGACACCT ATTGGAGAATTACATCTACAAGGGGCTTCAAACAGACCAAATAGATCATCACCTCTGTGGTCCCTTGTTAACTATATGTTCTGAGACAAAGGAAAGCTAC CCTAAGGGTTAGTTAACCTTTGCTGAGGAAATTTACATTCATACTTAGAGTGAATTACTCAGGTGTGCTTAGGTGTGCAAAAGGGAAGGAGACCTGAATT CACCAAGTTAAATCTTGCTAAACCTTATCATAAGCATTTTTTGAGCGCTTAGCATACACCAAGCCTTGTGGAAGGTGCTTTCCTGCCATATCTCATTTAA TCCTCACAGCAAACCTATAGAATATGGCATTATCATCTGAGTCTCACAGAAGTTTAGTCGTGTACTCAAGGTCTTACCAGCTAGTGAACAGCAGACCAAG ACTGGAAACCCAGGATAGTCTGATACCTGAGCCATCTCTTCTTGTGCTACGCCTAGTTATTCTGTCCCCCAAATCAAAAGGCATGACCTTTATAAGAGGC GCTTTACTGACAATAGCTGCAATTTTAACTTTGAAAATGATTCAGAATTATCAAAGATAGTAGATTCGAATGACATGATTGTCTATAATCTCGCTAGCCT TGTACTGTGTGTGCATAGCAATTACAGGGAAGTAATCTAGCTCCTGACTATTATGTTGAACTATGTCGCTGCTTTTTACAAACTTGTCTTGATCCAAAGC AGTCACAATGATAACCCTGCATATCTGGGAATCATAAGTCAACTATGTATCCCTGTGTGTGTATATATATGTATGTATGTATCTATTTTCAAACTGTGAT TTAATATTTAAATATTCCTACTGCCATTTTTGTGACTGAAAAACTACACATGAGGAAACGTCTTAGAATTTTCCAATAGAGGAAAAATAACACTTGGGCA ATCTGTCATGTTTCACAACAGTTCTCATTTTTCTCATGATTTGTGTAGCGTGGAATGTGTTTGCTCAATGTGAAGGGTTTTCATTGCTCAATTTCTCTGT GTAAGTCTTTTCCTTAAGGTAATAAACCATCAGCAAAGTCACATACTGGAGTTGGTGGCTTTTCTTGTACAGGCAGTTGTTATGAGACAATGATGGAGCA TTGAGCATGTTCAATAAATGTGCAGATGGTGGAAA

WISP1.u.f.1 (forward primer)
WISP1.u.r.4 (reverse primer)
Single exon skipping

Frame 0

ISGAPDGPASLGPAPPRGGRILWAARSMPVPLT SRHEVVPALDAGSSDSSSRQHRPGHGPLSSPYDH GLYPSSTGGHLLTPPILQVAM*VPAIPTPLPAG GQPHHRWL*VL*DVRSAAWGQLHGGCHL*PPPG PLL*LQRGPPEVRNRSVCTQGRSVWLCTSQRHP* TSHLRAASAHAPINPSTVEFAWTIGAASPTSLR LSTCPSSVLMGLASPARSYGLMPASVT*AVGIP MTSLLTWNPTLTSQKLPTRQAQILGLGD*PNACE AVSPYGQ*LFTNEP*LP*SGPLASISVSNHSND A*WCCSGPCYEFSP*YHSASTLKKNACL*LFWT TPKPDPAFPSH*KSCWILPKSQEMESGRLLISLI SSLDAKPQDSLGPFR*IDGIWNNRIIYYLEPAK RYCNG*F*RQRTKTILIPNMYAPQGHQTFAK*V E*LLNFDF*WKVVSINLGIVEVKFLFTPTL*RVQ IRFVPVRNKI**TFLLMGKAPS*YSRDRERSAR FRRTN*LSH*ISC*LAGLWAVGQALP*IFSLVL LGVHRNW*GLWTGLSGP*EWCPGTLLYSYRALRD PAADHARPTEMTKTGSGRGVGQTKKWVPLVAAW GDL*SWRLWDSRGPRVQDTSIAETHFTAFRSAD QMASFLVGRWRFTGCLETEIDLIKV*S*RG*S*K EKVVVNEYQAIIYCIRKI*YLLLEFFYLGPFLC QTLLSVLCMY*LTESSRQC*EVPIIISVLTNVK RKLIEVRKLNQSHPVGDWES*DSDRNWTVFITHI FPLFLELPNVSE*ENIAINGLIF*CHFSLL*SF *LLFKRREYLIF**FRCLSIQNTWDTQKSRNPL *LIPSFPSEPALITHLKDDSVYPMLHIECCVVIH REFCAVCREIPKREKDWEYILLTVTSPKPSPVQ GIQWCSLNT**IQEAVHVFLLLFEALFFQSYMK IEF*QSKFYIKCLSKRDI*YFKEMHMYVYIYLCM RMQEFLYKENSLHE*SLL*VSVNHVRFSESENT CHLQITRLDNSSLHLKFSGNPRARFLLNLHLNL GN*T*LSKIIGSLVGETRDYSGNGCRKVVRITQS PLVLRNGTILCRARRASLRPMG*SHGEGNLDPM **SAF*VRISLQWCGLIQ*KLRIINIMEKNLH* LSVYLVPSTMLSCSLFYLIVTAILSMHLSLFYKW KREVPPKS*SSNSSLFYQPLLLRRPLEKT*MTP IGELHLQGASNRPNRSSPLWSLVNYMF*DKGKL P*GLVNLC*GNLHSYLE*ITQVCLGVQKGRRPEF TKLNLAKPYHKHFLSA*HTPSLVEGAFLPYLI* SSQQTYRIWHYHLSLTEV*SCTQGLTS**TADQ DWKPRIV*YLSHLFLCYA*LFCPPNQKA*PL*EA LY*Q*LQF*L*K*FRIIKDSRFE*HDCL*SR*P CTVCA*QLQGSNLAPDYYVELCRCFLQTCLDPK QSQ**PCISGNHKSTMYPCVCIYMYVCIYFQTVI *YLNIPTAIFVTEKLHMRKRLRIFQ*RKNNTWA ICHVSQQFSFFS*FV*RGMCLLNVKGFHCSISL CKSFP*GNKPSAKSHTGVGGFSCTGSCYETMMEH *ACSINVQMVE

Frame 1

YLVLLMGRPVWAQLPREVVGSSGLLGRCLCH*R PGMRWFLPWTLAAVTAAAASTVLATALSPAPTT MDFTPAPLEDTSSRPQFCKWPCECPPSPPRCPLG VSLITDGCECCKMCAQQLGDNCTEAAICDPHRG LYCDYSGDRPRYAIGVCARREEVSGCVPARGIH ELHTCGLHQHTLLSTQVLWSLHGQ*VLHPLQV*D YRRVLPVS*WAWLLPPGPMD*CLLL*PEL*ESQ *HLC*LGILP*LLRNCQLGRHKSWVLGTNPMPV KQSALMANNFSPMSLSYPDLDPWPPFLSLTIQMT PDGAAQAHAMSFLLDIIQHLL*RKMPVSSCSGL HPSLIQPFQVTRSPAGSCLNPKKWNQVDF*YH* FLL*MPNHKTLWVHSDE*MEFGTIE*SIIWSLPR GTVMGNSDVSAPKLS*FQICMHLKVIKHLPSEL NSCLILIFNGKLYPLTWALLRLSFSSPLHCEGY RLGLSQSEIKFDKHSC*WEKPPVNTPETGKGQPV SEGPIDSHTESAADWQGFGQLARLFLESSPLSC LGFIGIGKASGLACLAPESGALEHSSTLTEP*E TQLQTMPDPLK*PRQVQVGVWVKPRSGCPW*QPG VTSRAGGCGTPGAPVFRTHLLQRLISQPFVLLT KWPVFW*EDGGLPVV*KQK*T**RFKAEEVEAK RKRLLLMNIRLLFIVLGKYNIYC*NSFI*GLFCA RHCSQCFACISSLNLHDNVEKFPLLFLFLQM*N GSS*R*ENSTRVTQLVTGKVRIQIEIGLSL*PI FSPCF*SFQMCQNRKTLQ*MA*FFNVIFPSYSLS SSFSKDENI*FSDNLGA*ASKIHGTHKNPGIPC SLFPLSHRNQLSSHI*KMILFTQCCILNVV*LF TGNSVQCAERFLNGKRTGNTSSLL*PPQNLVQCK VYSGAH*ILDEYRKLCMCSYFYSKLSSSKAT*K *NFNSQNFILSALAKETFNISKKCICMYTYICV CVCKNSCIKRIHSMNDLFCKSV*IMLDFLRVKTP AIYKLQGWITAHSI*NSVETQELGSY*ICISIW ETELSFQRS*EVWLEKLGIILAMGAGRWSE*PS RHWF*ETELSYAEPGGQVSDPWVEAMEKEIWIQC NEALSKSEFPCNGVA*FNKN*E**I*WKKISTD *VFTWCQALC*VVHYFI*LLQQF*VCIFHYFIS GKEKCPQKVRAQTAAYSTSPCSCGGLWKRPE*HL LENYIYKGLQTDQIDHHLCGPLLTICSETKESY PKG*LTFAEEIYIHT*SELLRCA*VCKREGDLN SPS*ILLNLIISIF*ALSIHQALWKVLSCHISFN PHSKPIEYGIII*VSQKFSRVLKVLPASEQQTK TGNPG*SDT*AISSCATPSYSVPQIKRHDLYKR RFTDNSCNFNFENDSELSKIVDSNDMIVYNLASL VLCVHSNYREVI*LLTIMLNYVAAFYKLVLIQS SHNDNPAYLGIISQLCIPVCVYICMYVSIFKL* FNI*IFLLPFL*LKNYT*GNVLEFSNRGKITLGQ SVMFHNSSHFSHDLCSVECVCSM*RVFIAQFLC VSLFLKVINHQQSHILELVAFLVQAVVMRQ*WS IEHVQ*MCRWWK

Frame 2

IWCS*WAGQSGPSSPERWSDPLGCSVDACATDV QA*GGSCPGRWQQ*QQQPPAPSWPRPSLQPLRP WTLPQLHWRTPPHAPNSASGHVSARHPHPAARW GSASSQMAVSAVRCALSSLGTTARRLPSVTPTGA STVTTAGTARGTQ*ECVHAGKKCLAVYQPEASM NFTLAGCISTRSYQPKYCGVCMDNRCCIPYKSK TIDVSFQCPDGLGFSRQVLWINACFCNLSCRNPN DIFADLESYPDFSEIAN*AGTNLGSWGLTQCL* SSQPLWPITFHQ*ALVTLIWTLGLHFCL*PFK* RLMVLLRPML*VFSLISFSIYSKEKCLSLAVLDY TQA*SSLSKSLEVLLDLA*IPRNGIR*TFNITN FFFRCQTTRLFGSIQMNRWNLEQ*NNLLFGACQ EVL*WVILTSAHQNYPDSKYVCTSRSSNICQVS* IVA*F*FLMESCIH*PGHC*G*VSLHPYTVKGT D*VCPSQK*NLINIPVDGKSPQLILQRQGKVSP FQKDQLTLTLNQLLTGRALGSWPGSSLNLLPCPA WGS*ELVRPLDWPVWPLRVVPWNTPLLLQSLER PSCRPCQTH*NDQDRFR*GCGSNQEVGALGSSL G*PLELEAVGLQGPPCSGHIYCRDSFHSLSFC*P NGQFSGRKMEVYRLFRNRNRLNKGLKLKRLKLK GKGCC**ISGYYLLY*ENIIFTVRILLFRAFSV PDIALSALHVLAH*IFTTMLRSSHYYFCSYKCET EAHRGEKTQPESPSW*LGKLGFRSKLDCLYNPY FPPVFRASKCVRIGKHCNKWLDFLMSFFPLIVF LAPFQKTRISDFLII*VLKHPKYMGHTKIQESPV AYSLFPIGTSSHHTFKR*FCLPNAAY*MLCSYS QGILCSVQRDS*TGKGLGIHPPYCDLPKT*SSA RYTVVLIKYLMNTGSCACVPTFIRSSLLPKLHEN RILTVKILY*VP*QKRHLIFQRNAYVCIHIFVY AYARILV*REFTP*MISSVSQCESC*IF*E*KH LPSTNYKAG*QLTPFEIQWKPKS*VLTEFASQFG KLNLAFKDHRKSGWRN*GLFWQWVQEGGQNNPV AIGFEKRNYLMQSPEGKSQTHGLKPWRRKFGSN VMKRFLSQNFPAMVWPDSIKIKNNKYNGKKSPLI ECLLGAKHYAKLFIILFNCYSNFEYASFTIL*V EKRSAPKKLELKQQLILPAPALAEASGKDLNDT YWRITSTRGFKQTK*IITSVVPC*LYVLRQRKAT LRVS*PLLRKFTFILRVNYSGVLRCAKGKET*I HQVKSC*TLS*AFFERLAYTKPCGRCFPAISHL ILTANL*NMALSSESHRSLVVYSRSYQLVNSRPR LETQDSLIPEPSLLVLRLVILSPKSKGMTFIRG ALLTIAAILTLKMIQNYQR**IRMT*LSIISLA LYCVCIAITGK*SSS*LLC*TMSLLFTNLS*SKA VTMITLHIWES*VNYVSLCVYIYVCMYLFSNCD LIFKYSYCHFCD*KTTHEETS*NFPIEEK*HLG NLSCFTTVLIFLMICVAWNVFAQCEGFSLLNFSV *VFSLR**TISKVTYWSWWLFLYRQLL*DNDGA LSMFNKCADGG