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Sequence WISP1.cAug05
ATATCTGGTGCTCCTGATGGGCCGGCCAGTCTGGGCCCAGCTCCCCCGAGAGGTGGTCGGATCCTCTGGGCTGCTCGGTCGATGCCTGTGCCACTGACGTCCAGGCATGAGGTGGTTCCTGCCCTGGACGCTGGCAGCAGTGACAGCAGCAGCCGCCAGCACCGTCCTGGCCACGATGCTGTGGGTGAGGTGGAGGCATG
GCACAGGAACTGCATAGCCTACACAAGCCCCTGGAGCCCTTGCTCCACCAGCTGCGGCCTGGGGGTCTCCACTCGGATCTCCAATGTTAACGCCCAGTGC
TGGCCTGAGCAAGAGAGCCGCCTCTGCAACTTGCGGCCATGCGATGTGGACATCCATACACTCATTAAGGCAGGGAAGAAGTGTCTGGCTGTGTACCAGC
CAGAGGCATCCATGAACTTCACACTTGCGGGCTGCATCAGCACACGCTCCTATCAACCCAAGTACTGTGGAGTTTGCATGGACAATAGGTGCTGCATCCC
CTACAAGTCTAAGACTATCGACGTGTCCTTCCAGTGTCCTGATGGGCTTGGCTTCTCCCGCCAGGTCCTATGGATTAATGCCTGCTTCTGTAACCTGAGC
TGTAGGAATCCCAATGACATCTTTGCTGACTTGGAATCCTACCCTGACTTCTCAGAAATTGCCAACTAGGCAGGCACAAATCTTGGGTCTTGGGGACTAA
CCCAATGCCTGTGAAGCAGTCAGCCCTTATGGCCAATAACTTTTCACCAATGAGCCTTAGTTACCCTGATCTGGACCCTTGGCCTCCATTTCTGTCTCTA
ACCATTCAAATGACGCCTGATGGTGCTGCTCAGGCCCATGCTATGAGTTTTCTCCTTGATATCATTCAGCATCTACTCTAAAGAAAAATGCCTGTCTCTA
GCTGTTCTGGACTACACCCAAGCCTGATCCAGCCTTTCCAAGTCACTAGAAGTCCTGCTGGATCTTGCCTAAATCCCAAGAAATGGAATCAGGTAGACTT
TTAATATCACTAATTTCTTCTTTAGATGCCAAACCACAAGACTCTTTGGGTCCATTCAGATGAATAGATGGAATTTGGAACAATAGAATAATCTATTATT
TGGAGCCTGCCAAGAGGTACTGTAATGGGTAATTCTGACGTCAGCGCACCAAAACTATCCTGATTCCAAATATGTATGCACCTCAAGGTCATCAAACATT
TGCCAAGTGAGTTGAATAGTTGCTTAATTTTGATTTTTAATGGAAAGTTGTATCCATTAACCTGGGCATTGTTGAGGTTAAGTTTCTCTTCACCCCTACA
CTGTGAAGGGTACAGATTAGGTTTGTCCCAGTCAGAAATAAAATTTGATAAACATTCCTGTTGATGGGAAAAGCCCCCAGTTAATACTCCAGAGACAGGG
AAAGGTCAGCCCGTTTCAGAAGGACCAATTGACTCTCACACTGAATCAGCTGCTGACTGGCAGGGCTTTGGGCAGTTGGCCAGGCTCTTCCTTGAATCTT
CTCCCTTGTCCTGCTTGGGGTTCATAGGAATTGGTAAGGCCTCTGGACTGGCCTGTCTGGCCCCTGAGAGTGGTGCCCTGGAACACTCCTCTACTCTTAC
AGAGCCTTGAGAGACCCAGCTGCAGACCATGCCAGACCCACTGAAATGACCAAGACAGGTTCAGGTAGGGGTGTGGGTCAAACCAAGAAGTGGGTGCCCT
TGGTAGCAGCCTGGGGTGACCTCTAGAGCTGGAGGCTGTGGGACTCCAGGGGCCCCCGTGTTCAGGACACATCTATTGCAGAGACTCATTTCACAGCCTT
TCGTTCTGCTGACCAAATGGCCAGTTTTCTGGTAGGAAGATGGAGGTTTACCGGTTGTTTAGAAACAGAAATAGACTTAATAAAGGTTTAAAGCTGAAGA
GGTTGAAGCTAAAAGGAAAAGGTTGTTGTTAATGAATATCAGGCTATTATTTATTGTATTAGGAAAATATAATATTTACTGTTAGAATTCTTTTATTTAG
GGCCTTTTCTGTGCCAGACATTGCTCTCAGTGCTTTGCATGTATTAGCTCACTGAATCTTCACGACAATGTTGAGAAGTTCCCATTATTATTTCTGTTCT
TACAAATGTGAAACGGAAGCTCATAGAGGTGAGAAAACTCAACCAGAGTCACCCAGTTGGTGACTGGGAAAGTTAGGATTCAGATCGAAATTGGACTGTC
TTTATAACCCATATTTTCCCCCTGTTTTTAGAGCTTCCAAATGTGTCAGAATAGGAAAACATTGCAATAAATGGCTTGATTTTTTAATGTCATTTTTCCC
TCTTATAGTCTTTCTAGCTCCTTTTCAAAAGACGAGAATATCTGATTTTCTGATAATTTAGGTGCTTAAGCATCCAAAATACATGGGACACACAAAAATC
CAGGAATCCCCTGTAGCTTATTCCCTCTTTCCCATCGGAACCAGCTCTCATCACACATTTAAAAGATGATTCTGTTTACCCAATGCTGCATATTGAATGT
TGTGTAGTTATTCACAGGGAATTCTGTGCAGTGTGCAGAGAGATTCCTAAACGGGAAAAGGACTGGGAATACATCCTCCTTACTGTGACCTCCCCAAAAC
CTAGTCCAGTGCAAGGTATACAGTGGTGCTCATTAAATACTTGATGAATACAGGAAGCTGTGCATGTGTTCCTACTTTTATTCGAAGCTCTCTTCTTCCA
AAGCTACATGAAAATAGAATTTTAACAGTCAAAATTTTATATTAAGTGCCTTAGCAAAAGAGACATTTAATATTTCAAAGAAATGCATATGTATGTATAC
ATATATTTGTGTATGCGTATGCAAGAATTCTTGTATAAAGAGAATTCACTCCATGAATGATCTCTTCTGTAAGTCAGTGTGAATCATGTTAGATTTTCTG
AGAGTGAAAACACCTGCCATCTACAAATTACAAGGCTGGATAACAGCTCACTCCATTTGAAATTCAGTGGAAACCCAAGAGCTAGGTTCTTACTGAATTT
GCATCTCAATTTGGGAAACTGAACTTAGCTTTCAAAGATCATAGGAAGTCTGGTTGGAGAAACTAGGGATTATTCTGGCAATGGGTGCAGGAAGGTGGTC
AGAATAACCCAGTCGCCATTGGTTTTGAGAAACGGAACTATCTTATGCAGAGCCCGGAGGGCAAGTCTCAGACCCATGGGTTGAAGCCATGGAGAAGGAA
ATTTGGATCCAATGTAATGAAGCGCTTTCTAAGTCAGAATTTCCCTGCAATGGTGTGGCCTGATTCAATAAAAATTAAGAATAATAAATATAATGGAAAA
AAATCTCCACTGATTGAGTGTTTACTTGGTGCCAAGCACTATGCTAAGTTGTTCATTATTTTATTTAATTGTTACAGCAATTTTGAGTATGCATCTTTCA
CTATTTTATAAGTGGAAAAGAGAAGTGCCCCCAAAAAGTTAGAGCTCAAACAGCAGCTTATTCTACCAGCCCCTGCTCTTGCGGAGGCCTCTGGAAAAGA
CCTGAATGACACCTATTGGAGAATTACATCTACAAGGGGCTTCAAACAGACCAAATAGATCATCACCTCTGTGGTCCCTTGTTAACTATATGTTCTGAGA
CAAAGGAAAGCTACCCTAAGGGTTAGTTAACCTTTGCTGAGGAAATTTACATTCATACTTAGAGTGAATTACTCAGGTGTGCTTAGGTGTGCAAAAGGGA
AGGAGACCTGAATTCACCAAGTTAAATCTTGCTAAACCTTATCATAAGCATTTTTTGAGCGCTTAGCATACACCAAGCCTTGTGGAAGGTGCTTTCCTGC
CATATCTCATTTAATCCTCACAGCAAACCTATAGAATATGGCATTATCATCTGAGTCTCACAGAAGTTTAGTCGTGTACTCAAGGTCTTACCAGCTAGTG
AACAGCAGACCAAGACTGGAAACCCAGGATAGTCTGATACCTGAGCCATCTCTTCTTGTGCTACGCCTAGTTATTCTGTCCCCCAAATCAAAAGGCATGA
CCTTTATAAGAGGCGCTTTACTGACAATAGCTGCAATTTTAACTTTGAAAATGATTCAGAATTATCAAAGATAGTAGATTCGAATGACATGATTGTCTAT
AATCTCGCTAGCCTTGTACTGTGTGTGCATAGCAATTACAGGGAAGTAATCTAGCTCCTGACTATTATGTTGAACTATGTCGCTGCTTTTTACAAACTTG
TCTTGATCCAAAGCAGTCACAATGATAACCCTGCATATCTGGGAATCATAAGTCAACTATGTATCCCTGTGTGTGTATATATATGTATGTATGTATCTAT
TTTCAAACTGTGATTTAATATTTAAATATTCCTACTGCCATTTTTGTGACTGAAAAACTACACATGAGGAAACGTCTTAGAATTTTCCAATAGAGGAAAA
ATAACACTTGGGCAATCTGTCATGTTTCACAACAGTTCTCATTTTTCTCATGATTTGTGTAGCGTGGAATGTGTTTGCTCAATGTGAAGGGTTTTCATTG
CTCAATTTCTCTGTGTAAGTCTTTTCCTTAAGGTAATAAACCATCAGCAAAGTCACATACTGGAGTTGGTGGCTTTTCTTGTACAGGCAGTTGTTATGAG
ACAATGATGGAGCATTGAGCATGTTCAATAAATGTGCAGATGGTGGAAA
- WISP1.u.f.1 (forward primer)
- WISP1.u.r.4 (reverse primer)
- Single exon skipping
Frame 0
ISGAPDGPASLGPAPPRGGRILWAARSMPVPLTSRHEVVPALDAGSSDSSSRQHRPGHDAVGEVEAW
HRNCIAYTSPWSPCSTSCGLGVSTRISNVNAQC
WPEQESRLCNLRPCDVDIHTLIKAGKKCLAVYQ
PEASMNFTLAGCISTRSYQPKYCGVCMDNRCCIP
YKSKTIDVSFQCPDGLGFSRQVLWINACFCNLS
CRNPNDIFADLESYPDFSEIAN*AGTNLGSWGL
TQCL*SSQPLWPITFHQ*ALVTLIWTLGLHFCL*
PFK*RLMVLLRPML*VFSLISFSIYSKEKCLSL
AVLDYTQA*SSLSKSLEVLLDLA*IPRNGIR*T
FNITNFFFRCQTTRLFGSIQMNRWNLEQ*NNLLF
GACQEVL*WVILTSAHQNYPDSKYVCTSRSSNI
CQVS*IVA*F*FLMESCIH*PGHC*G*VSLHPY
TVKGTD*VCPSQK*NLINIPVDGKSPQLILQRQG
KVSPFQKDQLTLTLNQLLTGRALGSWPGSSLNL
LPCPAWGS*ELVRPLDWPVWPLRVVPWNTPLLL
QSLERPSCRPCQTH*NDQDRFR*GCGSNQEVGAL
GSSLG*PLELEAVGLQGPPCSGHIYCRDSFHSL
SFC*PNGQFSGRKMEVYRLFRNRNRLNKGLKLK
RLKLKGKGCC**ISGYYLLY*ENIIFTVRILLFR
AFSVPDIALSALHVLAH*IFTTMLRSSHYYFCS
YKCETEAHRGEKTQPESPSW*LGKLGFRSKLDC
LYNPYFPPVFRASKCVRIGKHCNKWLDFLMSFFP
LIVFLAPFQKTRISDFLII*VLKHPKYMGHTKI
QESPVAYSLFPIGTSSHHTFKR*FCLPNAAY*M
LCSYSQGILCSVQRDS*TGKGLGIHPPYCDLPKT
*SSARYTVVLIKYLMNTGSCACVPTFIRSSLLP
KLHENRILTVKILY*VP*QKRHLIFQRNAYVCI
HIFVYAYARILV*REFTP*MISSVSQCESC*IF*
E*KHLPSTNYKAG*QLTPFEIQWKPKS*VLTEF
ASQFGKLNLAFKDHRKSGWRN*GLFWQWVQEGG
QNNPVAIGFEKRNYLMQSPEGKSQTHGLKPWRRK
FGSNVMKRFLSQNFPAMVWPDSIKIKNNKYNGK
KSPLIECLLGAKHYAKLFIILFNCYSNFEYASF
TIL*VEKRSAPKKLELKQQLILPAPALAEASGKD
LNDTYWRITSTRGFKQTK*IITSVVPC*LYVLR
QRKATLRVS*PLLRKFTFILRVNYSGVLRCAKG
KET*IHQVKSC*TLS*AFFERLAYTKPCGRCFPA
ISHLILTANL*NMALSSESHRSLVVYSRSYQLV
NSRPRLETQDSLIPEPSLLVLRLVILSPKSKGM
TFIRGALLTIAAILTLKMIQNYQR**IRMT*LSI
ISLALYCVCIAITGK*SSS*LLC*TMSLLFTNL
S*SKAVTMITLHIWES*VNYVSLCVYIYVCMYL
FSNCDLIFKYSYCHFCD*KTTHEETS*NFPIEEK
*HLGNLSCFTTVLIFLMICVAWNVFAQCEGFSL
LNFSV*VFSLR**TISKVTYWSWWLFLYRQLL*
DNDGALSMFNKCADGG
Frame 1
YLVLLMGRPVWAQLPREVVGSSGLLGRCLCH*RPGMRWFLPWTLAAVTAAAASTVLATMLWVRWRH
GTGTA*PTQAPGALAPPAAAWGSPLGSPMLTPSA
GLSKRAASATCGHAMWTSIHSLRQGRSVWLCTS
QRHP*TSHLRAASAHAPINPSTVEFAWTIGAAS
PTSLRLSTCPSSVLMGLASPARSYGLMPASVT*A
VGIPMTSLLTWNPTLTSQKLPTRQAQILGLGD*
PNACEAVSPYGQ*LFTNEP*LP*SGPLASISVS
NHSNDA*WCCSGPCYEFSP*YHSASTLKKNACL*
LFWTTPKPDPAFPSH*KSCWILPKSQEMESGRL
LISLISSLDAKPQDSLGPFR*IDGIWNNRIIYY
LEPAKRYCNG*F*RQRTKTILIPNMYAPQGHQTF
AK*VE*LLNFDF*WKVVSINLGIVEVKFLFTPT
L*RVQIRFVPVRNKI**TFLLMGKAPS*YSRDR
ERSARFRRTN*LSH*ISC*LAGLWAVGQALP*IF
SLVLLGVHRNW*GLWTGLSGP*EWCPGTLLYSY
RALRDPAADHARPTEMTKTGSGRGVGQTKKWVP
LVAAWGDL*SWRLWDSRGPRVQDTSIAETHFTAF
RSADQMASFLVGRWRFTGCLETEIDLIKV*S*R
G*S*KEKVVVNEYQAIIYCIRKI*YLLLEFFYL
GPFLCQTLLSVLCMY*LTESSRQC*EVPIIISVL
TNVKRKLIEVRKLNQSHPVGDWES*DSDRNWTV
FITHIFPLFLELPNVSE*ENIAINGLIF*CHFS
LL*SF*LLFKRREYLIF**FRCLSIQNTWDTQKS
RNPL*LIPSFPSEPALITHLKDDSVYPMLHIEC
CVVIHREFCAVCREIPKREKDWEYILLTVTSPK
PSPVQGIQWCSLNT**IQEAVHVFLLLFEALFFQ
SYMKIEF*QSKFYIKCLSKRDI*YFKEMHMYVY
IYLCMRMQEFLYKENSLHE*SLL*VSVNHVRFS
ESENTCHLQITRLDNSSLHLKFSGNPRARFLLNL
HLNLGN*T*LSKIIGSLVGETRDYSGNGCRKVV
RITQSPLVLRNGTILCRARRASLRPMG*SHGEG
NLDPM**SAF*VRISLQWCGLIQ*KLRIINIMEK
NLH*LSVYLVPSTMLSCSLFYLIVTAILSMHLS
LFYKWKREVPPKS*SSNSSLFYQPLLLRRPLEK
T*MTPIGELHLQGASNRPNRSSPLWSLVNYMF*D
KGKLP*GLVNLC*GNLHSYLE*ITQVCLGVQKG
RRPEFTKLNLAKPYHKHFLSA*HTPSLVEGAFL
PYLI*SSQQTYRIWHYHLSLTEV*SCTQGLTS**
TADQDWKPRIV*YLSHLFLCYA*LFCPPNQKA*
PL*EALY*Q*LQF*L*K*FRIIKDSRFE*HDCL
*SR*PCTVCA*QLQGSNLAPDYYVELCRCFLQTC
LDPKQSQ**PCISGNHKSTMYPCVCIYMYVCIY
FQTVI*YLNIPTAIFVTEKLHMRKRLRIFQ*RK
NNTWAICHVSQQFSFFS*FV*RGMCLLNVKGFHC
SISLCKSFP*GNKPSAKSHTGVGGFSCTGSCYE
TMMEH*ACSINVQMVE
Frame 2
IWCS*WAGQSGPSSPERWSDPLGCSVDACATDVQA*GGSCPGRWQQ*QQQPPAPSWPRCCG*GGGM
AQELHSLHKPLEPLLHQLRPGGLHSDLQC*RPV
LA*AREPPLQLAAMRCGHPYTH*GREEVSGCVPA
RGIHELHTCGLHQHTLLSTQVLWSLHGQ*VLHP
LQV*DYRRVLPVS*WAWLLPPGPMD*CLLL*PE
L*ESQ*HLC*LGILP*LLRNCQLGRHKSWVLGTN
PMPVKQSALMANNFSPMSLSYPDLDPWPPFLSL
TIQMTPDGAAQAHAMSFLLDIIQHLL*RKMPVS
SCSGLHPSLIQPFQVTRSPAGSCLNPKKWNQVDF
*YH*FLL*MPNHKTLWVHSDE*MEFGTIE*SII
WSLPRGTVMGNSDVSAPKLS*FQICMHLKVIKH
LPSELNSCLILIFNGKLYPLTWALLRLSFSSPLH
CEGYRLGLSQSEIKFDKHSC*WEKPPVNTPETG
KGQPVSEGPIDSHTESAADWQGFGQLARLFLES
SPLSCLGFIGIGKASGLACLAPESGALEHSSTLT
EP*ETQLQTMPDPLK*PRQVQVGVWVKPRSGCP
W*QPGVTSRAGGCGTPGAPVFRTHLLQRLISQP
FVLLTKWPVFW*EDGGLPVV*KQK*T**RFKAEE
VEAKRKRLLLMNIRLLFIVLGKYNIYC*NSFI*
GLFCARHCSQCFACISSLNLHDNVEKFPLLFLF
LQM*NGSS*R*ENSTRVTQLVTGKVRIQIEIGLS
L*PIFSPCF*SFQMCQNRKTLQ*MA*FFNVIFP
SYSLSSSFSKDENI*FSDNLGA*ASKIHGTHKN
PGIPCSLFPLSHRNQLSSHI*KMILFTQCCILNV
V*LFTGNSVQCAERFLNGKRTGNTSSLL*PPQN
LVQCKVYSGAH*ILDEYRKLCMCSYFYSKLSSS
KAT*K*NFNSQNFILSALAKETFNISKKCICMYT
YICVCVCKNSCIKRIHSMNDLFCKSV*IMLDFL
RVKTPAIYKLQGWITAHSI*NSVETQELGSY*I
CISIWETELSFQRS*EVWLEKLGIILAMGAGRWS
E*PSRHWF*ETELSYAEPGGQVSDPWVEAMEKE
IWIQCNEALSKSEFPCNGVA*FNKN*E**I*WK
KISTD*VFTWCQALC*VVHYFI*LLQQF*VCIFH
YFISGKEKCPQKVRAQTAAYSTSPCSCGGLWKR
PE*HLLENYIYKGLQTDQIDHHLCGPLLTICSE
TKESYPKG*LTFAEEIYIHT*SELLRCA*VCKRE
GDLNSPS*ILLNLIISIF*ALSIHQALWKVLSC
HISFNPHSKPIEYGIII*VSQKFSRVLKVLPAS
EQQTKTGNPG*SDT*AISSCATPSYSVPQIKRHD
LYKRRFTDNSCNFNFENDSELSKIVDSNDMIVY
NLASLVLCVHSNYREVI*LLTIMLNYVAAFYKL
VLIQSSHNDNPAYLGIISQLCIPVCVYICMYVSI
FKL*FNI*IFLLPFL*LKNYT*GNVLEFSNRGK
ITLGQSVMFHNSSHFSHDLCSVECVCSM*RVFI
AQFLCVSLFLKVINHQQSHILELVAFLVQAVVMR
Q*WSIEHVQ*MCRWWK
Sequence WISP1.bAug05
ATATCTGGTGCTCCTGATGGGCCGGCCAGTCTGGGCCCAGCTCCCCCGAGAGGTGGTCGGATCCTCTGGGCTGCTCGGTCGATGCCTGTGCCACTGACGTCCAGGCATGAGGTGGTTCCTGCCCTGGACGCTGGCAGCAGTGACAGCAGCAGCCGCCAGCACCGTCCTGGCCACGGCCCTCTCTCCAGCCCCTACGACCA
TGGACTTTACCCCAGCTCCACTGGAGGACACCTCCTCACGCCCCCAATTCTGCAAGTGGCCATGTGAGTGCCCGCCATCCCCACCCCGCTGCCCGCTGGG
GGTCAGCCTCATCACAGATGGCTGTGAGTGCTGTAAGATGTGCGCTCAGCAGCTTGGGGACAACTGCACGGAGGCTGCCATCTGTGACCCCCACCGGGGC
CTCTACTGTGACTACAGCGGGGACCGCCCGAGGTACGCAATAGGAGTGTGTGCACATGCTGTGGGTGAGGTGGAGGCATGGCACAGGAACTGCATAGCCT
ACACAAGCCCCTGGAGCCCTTGCTCCACCAGCTGCGGCCTGGGGGTCTCCACTCGGATCTCCAATGTTAACGCCCAGTGCTGGCCTGAGCAAGAGAGCCG
CCTCTGCAACTTGCGGCCATGCGATGTGGACATCCATACACTCATTAAGGCAGGGAAGAAGTGTCTGGCTGTGTACCAGCCAGAGGCATCCATGAACTTC
ACACTTGCGGGCTGCATCAGCACACGCTCCTATCAACCCAAGTACTGTGGAGTTTGCATGGACAATAGGTGCTGCATCCCCTACAAGTCTAAGACTATCG
ACGTGTCCTTCCAGTGTCCTGATGGGCTTGGCTTCTCCCGCCAGGTCCTATGGATTAATGCCTGCTTCTGTAACCTGAGCTGTAGGAATCCCAATGACAT
CTTTGCTGACTTGGAATCCTACCCTGACTTCTCAGAAATTGCCAACTAGGCAGGCACAAATCTTGGGTCTTGGGGACTAACCCAATGCCTGTGAAGCAGT
CAGCCCTTATGGCCAATAACTTTTCACCAATGAGCCTTAGTTACCCTGATCTGGACCCTTGGCCTCCATTTCTGTCTCTAACCATTCAAATGACGCCTGA
TGGTGCTGCTCAGGCCCATGCTATGAGTTTTCTCCTTGATATCATTCAGCATCTACTCTAAAGAAAAATGCCTGTCTCTAGCTGTTCTGGACTACACCCA
AGCCTGATCCAGCCTTTCCAAGTCACTAGAAGTCCTGCTGGATCTTGCCTAAATCCCAAGAAATGGAATCAGGTAGACTTTTAATATCACTAATTTCTTC
TTTAGATGCCAAACCACAAGACTCTTTGGGTCCATTCAGATGAATAGATGGAATTTGGAACAATAGAATAATCTATTATTTGGAGCCTGCCAAGAGGTAC
TGTAATGGGTAATTCTGACGTCAGCGCACCAAAACTATCCTGATTCCAAATATGTATGCACCTCAAGGTCATCAAACATTTGCCAAGTGAGTTGAATAGT
TGCTTAATTTTGATTTTTAATGGAAAGTTGTATCCATTAACCTGGGCATTGTTGAGGTTAAGTTTCTCTTCACCCCTACACTGTGAAGGGTACAGATTAG
GTTTGTCCCAGTCAGAAATAAAATTTGATAAACATTCCTGTTGATGGGAAAAGCCCCCAGTTAATACTCCAGAGACAGGGAAAGGTCAGCCCGTTTCAGA
AGGACCAATTGACTCTCACACTGAATCAGCTGCTGACTGGCAGGGCTTTGGGCAGTTGGCCAGGCTCTTCCTTGAATCTTCTCCCTTGTCCTGCTTGGGG
TTCATAGGAATTGGTAAGGCCTCTGGACTGGCCTGTCTGGCCCCTGAGAGTGGTGCCCTGGAACACTCCTCTACTCTTACAGAGCCTTGAGAGACCCAGC
TGCAGACCATGCCAGACCCACTGAAATGACCAAGACAGGTTCAGGTAGGGGTGTGGGTCAAACCAAGAAGTGGGTGCCCTTGGTAGCAGCCTGGGGTGAC
CTCTAGAGCTGGAGGCTGTGGGACTCCAGGGGCCCCCGTGTTCAGGACACATCTATTGCAGAGACTCATTTCACAGCCTTTCGTTCTGCTGACCAAATGG
CCAGTTTTCTGGTAGGAAGATGGAGGTTTACCGGTTGTTTAGAAACAGAAATAGACTTAATAAAGGTTTAAAGCTGAAGAGGTTGAAGCTAAAAGGAAAA
GGTTGTTGTTAATGAATATCAGGCTATTATTTATTGTATTAGGAAAATATAATATTTACTGTTAGAATTCTTTTATTTAGGGCCTTTTCTGTGCCAGACA
TTGCTCTCAGTGCTTTGCATGTATTAGCTCACTGAATCTTCACGACAATGTTGAGAAGTTCCCATTATTATTTCTGTTCTTACAAATGTGAAACGGAAGC
TCATAGAGGTGAGAAAACTCAACCAGAGTCACCCAGTTGGTGACTGGGAAAGTTAGGATTCAGATCGAAATTGGACTGTCTTTATAACCCATATTTTCCC
CCTGTTTTTAGAGCTTCCAAATGTGTCAGAATAGGAAAACATTGCAATAAATGGCTTGATTTTTTAATGTCATTTTTCCCTCTTATAGTCTTTCTAGCTC
CTTTTCAAAAGACGAGAATATCTGATTTTCTGATAATTTAGGTGCTTAAGCATCCAAAATACATGGGACACACAAAAATCCAGGAATCCCCTGTAGCTTA
TTCCCTCTTTCCCATCGGAACCAGCTCTCATCACACATTTAAAAGATGATTCTGTTTACCCAATGCTGCATATTGAATGTTGTGTAGTTATTCACAGGGA
ATTCTGTGCAGTGTGCAGAGAGATTCCTAAACGGGAAAAGGACTGGGAATACATCCTCCTTACTGTGACCTCCCCAAAACCTAGTCCAGTGCAAGGTATA
CAGTGGTGCTCATTAAATACTTGATGAATACAGGAAGCTGTGCATGTGTTCCTACTTTTATTCGAAGCTCTCTTCTTCCAAAGCTACATGAAAATAGAAT
TTTAACAGTCAAAATTTTATATTAAGTGCCTTAGCAAAAGAGACATTTAATATTTCAAAGAAATGCATATGTATGTATACATATATTTGTGTATGCGTAT
GCAAGAATTCTTGTATAAAGAGAATTCACTCCATGAATGATCTCTTCTGTAAGTCAGTGTGAATCATGTTAGATTTTCTGAGAGTGAAAACACCTGCCAT
CTACAAATTACAAGGCTGGATAACAGCTCACTCCATTTGAAATTCAGTGGAAACCCAAGAGCTAGGTTCTTACTGAATTTGCATCTCAATTTGGGAAACT
GAACTTAGCTTTCAAAGATCATAGGAAGTCTGGTTGGAGAAACTAGGGATTATTCTGGCAATGGGTGCAGGAAGGTGGTCAGAATAACCCAGTCGCCATT
GGTTTTGAGAAACGGAACTATCTTATGCAGAGCCCGGAGGGCAAGTCTCAGACCCATGGGTTGAAGCCATGGAGAAGGAAATTTGGATCCAATGTAATGA
AGCGCTTTCTAAGTCAGAATTTCCCTGCAATGGTGTGGCCTGATTCAATAAAAATTAAGAATAATAAATATAATGGAAAAAAATCTCCACTGATTGAGTG
TTTACTTGGTGCCAAGCACTATGCTAAGTTGTTCATTATTTTATTTAATTGTTACAGCAATTTTGAGTATGCATCTTTCACTATTTTATAAGTGGAAAAG
AGAAGTGCCCCCAAAAAGTTAGAGCTCAAACAGCAGCTTATTCTACCAGCCCCTGCTCTTGCGGAGGCCTCTGGAAAAGACCTGAATGACACCTATTGGA
GAATTACATCTACAAGGGGCTTCAAACAGACCAAATAGATCATCACCTCTGTGGTCCCTTGTTAACTATATGTTCTGAGACAAAGGAAAGCTACCCTAAG
GGTTAGTTAACCTTTGCTGAGGAAATTTACATTCATACTTAGAGTGAATTACTCAGGTGTGCTTAGGTGTGCAAAAGGGAAGGAGACCTGAATTCACCAA
GTTAAATCTTGCTAAACCTTATCATAAGCATTTTTTGAGCGCTTAGCATACACCAAGCCTTGTGGAAGGTGCTTTCCTGCCATATCTCATTTAATCCTCA
CAGCAAACCTATAGAATATGGCATTATCATCTGAGTCTCACAGAAGTTTAGTCGTGTACTCAAGGTCTTACCAGCTAGTGAACAGCAGACCAAGACTGGA
AACCCAGGATAGTCTGATACCTGAGCCATCTCTTCTTGTGCTACGCCTAGTTATTCTGTCCCCCAAATCAAAAGGCATGACCTTTATAAGAGGCGCTTTA
CTGACAATAGCTGCAATTTTAACTTTGAAAATGATTCAGAATTATCAAAGATAGTAGATTCGAATGACATGATTGTCTATAATCTCGCTAGCCTTGTACT
GTGTGTGCATAGCAATTACAGGGAAGTAATCTAGCTCCTGACTATTATGTTGAACTATGTCGCTGCTTTTTACAAACTTGTCTTGATCCAAAGCAGTCAC
AATGATAACCCTGCATATCTGGGAATCATAAGTCAACTATGTATCCCTGTGTGTGTATATATATGTATGTATGTATCTATTTTCAAACTGTGATTTAATA
TTTAAATATTCCTACTGCCATTTTTGTGACTGAAAAACTACACATGAGGAAACGTCTTAGAATTTTCCAATAGAGGAAAAATAACACTTGGGCAATCTGT
CATGTTTCACAACAGTTCTCATTTTTCTCATGATTTGTGTAGCGTGGAATGTGTTTGCTCAATGTGAAGGGTTTTCATTGCTCAATTTCTCTGTGTAAGT
CTTTTCCTTAAGGTAATAAACCATCAGCAAAGTCACATACTGGAGTTGGTGGCTTTTCTTGTACAGGCAGTTGTTATGAGACAATGATGGAGCATTGAGC
ATGTTCAATAAATGTGCAGATGGTGGAAA
- WISP1.u.f.1 (forward primer)
- WISP1.u.r.4 (reverse primer)
- Single exon skipping
Frame 0
ISGAPDGPASLGPAPPRGGRILWAARSMPVPLTSRHEVVPALDAGSSDSSSRQHRPGHGPLSSPYDH
GLYPSSTGGHLLTPPILQVAM*VPAIPTPLPAG
GQPHHRWL*VL*DVRSAAWGQLHGGCHL*PPPG
PLL*LQRGPPEVRNRSVCTCCG*GGGMAQELHSL
HKPLEPLLHQLRPGGLHSDLQC*RPVLA*AREP
PLQLAAMRCGHPYTH*GREEVSGCVPARGIHEL
HTCGLHQHTLLSTQVLWSLHGQ*VLHPLQV*DYR
RVLPVS*WAWLLPPGPMD*CLLL*PEL*ESQ*H
LC*LGILP*LLRNCQLGRHKSWVLGTNPMPVKQ
SALMANNFSPMSLSYPDLDPWPPFLSLTIQMTPD
GAAQAHAMSFLLDIIQHLL*RKMPVSSCSGLHP
SLIQPFQVTRSPAGSCLNPKKWNQVDF*YH*FL
L*MPNHKTLWVHSDE*MEFGTIE*SIIWSLPRGT
VMGNSDVSAPKLS*FQICMHLKVIKHLPSELNS
CLILIFNGKLYPLTWALLRLSFSSPLHCEGYRL
GLSQSEIKFDKHSC*WEKPPVNTPETGKGQPVSE
GPIDSHTESAADWQGFGQLARLFLESSPLSCLG
FIGIGKASGLACLAPESGALEHSSTLTEP*ETQ
LQTMPDPLK*PRQVQVGVWVKPRSGCPW*QPGVT
SRAGGCGTPGAPVFRTHLLQRLISQPFVLLTKW
PVFW*EDGGLPVV*KQK*T**RFKAEEVEAKRK
RLLLMNIRLLFIVLGKYNIYC*NSFI*GLFCARH
CSQCFACISSLNLHDNVEKFPLLFLFLQM*NGS
S*R*ENSTRVTQLVTGKVRIQIEIGLSL*PIFS
PCF*SFQMCQNRKTLQ*MA*FFNVIFPSYSLSSS
FSKDENI*FSDNLGA*ASKIHGTHKNPGIPCSL
FPLSHRNQLSSHI*KMILFTQCCILNVV*LFTG
NSVQCAERFLNGKRTGNTSSLL*PPQNLVQCKVY
SGAH*ILDEYRKLCMCSYFYSKLSSSKAT*K*N
FNSQNFILSALAKETFNISKKCICMYTYICVCV
CKNSCIKRIHSMNDLFCKSV*IMLDFLRVKTPAI
YKLQGWITAHSI*NSVETQELGSY*ICISIWET
ELSFQRS*EVWLEKLGIILAMGAGRWSE*PSRH
WF*ETELSYAEPGGQVSDPWVEAMEKEIWIQCNE
ALSKSEFPCNGVA*FNKN*E**I*WKKISTD*V
FTWCQALC*VVHYFI*LLQQF*VCIFHYFISGK
EKCPQKVRAQTAAYSTSPCSCGGLWKRPE*HLLE
NYIYKGLQTDQIDHHLCGPLLTICSETKESYPK
G*LTFAEEIYIHT*SELLRCA*VCKREGDLNSP
S*ILLNLIISIF*ALSIHQALWKVLSCHISFNPH
SKPIEYGIII*VSQKFSRVLKVLPASEQQTKTG
NPG*SDT*AISSCATPSYSVPQIKRHDLYKRRF
TDNSCNFNFENDSELSKIVDSNDMIVYNLASLVL
CVHSNYREVI*LLTIMLNYVAAFYKLVLIQSSH
NDNPAYLGIISQLCIPVCVYICMYVSIFKL*FN
I*IFLLPFL*LKNYT*GNVLEFSNRGKITLGQSV
MFHNSSHFSHDLCSVECVCSM*RVFIAQFLCVS
LFLKVINHQQSHILELVAFLVQAVVMRQ*WSIE
HVQ*MCRWWK
Frame 1
YLVLLMGRPVWAQLPREVVGSSGLLGRCLCH*RPGMRWFLPWTLAAVTAAAASTVLATALSPAPTT
MDFTPAPLEDTSSRPQFCKWPCECPPSPPRCPLG
VSLITDGCECCKMCAQQLGDNCTEAAICDPHRG
LYCDYSGDRPRYAIGVCAHAVGEVEAWHRNCIA
YTSPWSPCSTSCGLGVSTRISNVNAQCWPEQESR
LCNLRPCDVDIHTLIKAGKKCLAVYQPEASMNF
TLAGCISTRSYQPKYCGVCMDNRCCIPYKSKTI
DVSFQCPDGLGFSRQVLWINACFCNLSCRNPNDI
FADLESYPDFSEIAN*AGTNLGSWGLTQCL*SS
QPLWPITFHQ*ALVTLIWTLGLHFCL*PFK*RL
MVLLRPML*VFSLISFSIYSKEKCLSLAVLDYTQ
A*SSLSKSLEVLLDLA*IPRNGIR*TFNITNFF
FRCQTTRLFGSIQMNRWNLEQ*NNLLFGACQEV
L*WVILTSAHQNYPDSKYVCTSRSSNICQVS*IV
A*F*FLMESCIH*PGHC*G*VSLHPYTVKGTD*
VCPSQK*NLINIPVDGKSPQLILQRQGKVSPFQ
KDQLTLTLNQLLTGRALGSWPGSSLNLLPCPAWG
S*ELVRPLDWPVWPLRVVPWNTPLLLQSLERPS
CRPCQTH*NDQDRFR*GCGSNQEVGALGSSLG*
PLELEAVGLQGPPCSGHIYCRDSFHSLSFC*PNG
QFSGRKMEVYRLFRNRNRLNKGLKLKRLKLKGK
GCC**ISGYYLLY*ENIIFTVRILLFRAFSVPD
IALSALHVLAH*IFTTMLRSSHYYFCSYKCETEA
HRGEKTQPESPSW*LGKLGFRSKLDCLYNPYFP
PVFRASKCVRIGKHCNKWLDFLMSFFPLIVFLA
PFQKTRISDFLII*VLKHPKYMGHTKIQESPVAY
SLFPIGTSSHHTFKR*FCLPNAAY*MLCSYSQG
ILCSVQRDS*TGKGLGIHPPYCDLPKT*SSARY
TVVLIKYLMNTGSCACVPTFIRSSLLPKLHENRI
LTVKILY*VP*QKRHLIFQRNAYVCIHIFVYAY
ARILV*REFTP*MISSVSQCESC*IF*E*KHLP
STNYKAG*QLTPFEIQWKPKS*VLTEFASQFGKL
NLAFKDHRKSGWRN*GLFWQWVQEGGQNNPVAI
GFEKRNYLMQSPEGKSQTHGLKPWRRKFGSNVM
KRFLSQNFPAMVWPDSIKIKNNKYNGKKSPLIEC
LLGAKHYAKLFIILFNCYSNFEYASFTIL*VEK
RSAPKKLELKQQLILPAPALAEASGKDLNDTYW
RITSTRGFKQTK*IITSVVPC*LYVLRQRKATLR
VS*PLLRKFTFILRVNYSGVLRCAKGKET*IHQ
VKSC*TLS*AFFERLAYTKPCGRCFPAISHLIL
TANL*NMALSSESHRSLVVYSRSYQLVNSRPRLE
TQDSLIPEPSLLVLRLVILSPKSKGMTFIRGAL
LTIAAILTLKMIQNYQR**IRMT*LSIISLALY
CVCIAITGK*SSS*LLC*TMSLLFTNLS*SKAVT
MITLHIWES*VNYVSLCVYIYVCMYLFSNCDLI
FKYSYCHFCD*KTTHEETS*NFPIEEK*HLGNL
SCFTTVLIFLMICVAWNVFAQCEGFSLLNFSV*V
FSLR**TISKVTYWSWWLFLYRQLL*DNDGALS
MFNKCADGG
Frame 2
IWCS*WAGQSGPSSPERWSDPLGCSVDACATDVQA*GGSCPGRWQQ*QQQPPAPSWPRPSLQPLRP
WTLPQLHWRTPPHAPNSASGHVSARHPHPAARW
GSASSQMAVSAVRCALSSLGTTARRLPSVTPTGA
STVTTAGTARGTQ*ECVHMLWVRWRHGTGTA*P
TQAPGALAPPAAAWGSPLGSPMLTPSAGLSKRA
ASATCGHAMWTSIHSLRQGRSVWLCTSQRHP*TS
HLRAASAHAPINPSTVEFAWTIGAASPTSLRLS
TCPSSVLMGLASPARSYGLMPASVT*AVGIPMT
SLLTWNPTLTSQKLPTRQAQILGLGD*PNACEAV
SPYGQ*LFTNEP*LP*SGPLASISVSNHSNDA*
WCCSGPCYEFSP*YHSASTLKKNACL*LFWTTP
KPDPAFPSH*KSCWILPKSQEMESGRLLISLISS
LDAKPQDSLGPFR*IDGIWNNRIIYYLEPAKRY
CNG*F*RQRTKTILIPNMYAPQGHQTFAK*VE*
LLNFDF*WKVVSINLGIVEVKFLFTPTL*RVQIR
FVPVRNKI**TFLLMGKAPS*YSRDRERSARFR
RTN*LSH*ISC*LAGLWAVGQALP*IFSLVLLG
VHRNW*GLWTGLSGP*EWCPGTLLYSYRALRDPA
ADHARPTEMTKTGSGRGVGQTKKWVPLVAAWGD
L*SWRLWDSRGPRVQDTSIAETHFTAFRSADQM
ASFLVGRWRFTGCLETEIDLIKV*S*RG*S*KEK
VVVNEYQAIIYCIRKI*YLLLEFFYLGPFLCQT
LLSVLCMY*LTESSRQC*EVPIIISVLTNVKRK
LIEVRKLNQSHPVGDWES*DSDRNWTVFITHIFP
LFLELPNVSE*ENIAINGLIF*CHFSLL*SF*L
LFKRREYLIF**FRCLSIQNTWDTQKSRNPL*L
IPSFPSEPALITHLKDDSVYPMLHIECCVVIHRE
FCAVCREIPKREKDWEYILLTVTSPKPSPVQGI
QWCSLNT**IQEAVHVFLLLFEALFFQSYMKIE
F*QSKFYIKCLSKRDI*YFKEMHMYVYIYLCMRM
QEFLYKENSLHE*SLL*VSVNHVRFSESENTCH
LQITRLDNSSLHLKFSGNPRARFLLNLHLNLGN
*T*LSKIIGSLVGETRDYSGNGCRKVVRITQSPL
VLRNGTILCRARRASLRPMG*SHGEGNLDPM**
SAF*VRISLQWCGLIQ*KLRIINIMEKNLH*LS
VYLVPSTMLSCSLFYLIVTAILSMHLSLFYKWKR
EVPPKS*SSNSSLFYQPLLLRRPLEKT*MTPIG
ELHLQGASNRPNRSSPLWSLVNYMF*DKGKLP*
GLVNLC*GNLHSYLE*ITQVCLGVQKGRRPEFTK
LNLAKPYHKHFLSA*HTPSLVEGAFLPYLI*SS
QQTYRIWHYHLSLTEV*SCTQGLTS**TADQDW
KPRIV*YLSHLFLCYA*LFCPPNQKA*PL*EALY
*Q*LQF*L*K*FRIIKDSRFE*HDCL*SR*PCT
VCA*QLQGSNLAPDYYVELCRCFLQTCLDPKQS
Q**PCISGNHKSTMYPCVCIYMYVCIYFQTVI*Y
LNIPTAIFVTEKLHMRKRLRIFQ*RKNNTWAIC
HVSQQFSFFS*FV*RGMCLLNVKGFHCSISLCK
SFP*GNKPSAKSHTGVGGFSCTGSCYETMMEH*A
CSINVQMVE
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