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Sequences

Sequence ERCC2.jAug05

ERCC2.u.f.11 (forward primer)
ERCC2.u.r.11 (reverse primer)
Exon skipping and alternative donor or acceptor

Frame 0

AILLKLISNSWVRSILRQSWQAQEDQILVNEIE SLGSFPSMFSF*LALDDPQ*RLPHRLTRESAALA FPCARAVSRLALP*RIDGQVSQWSRNIGGASPR GCPRPGLSLNIHEGGGSTPLHSPAGAMK*EGGW GSRSLAGAGGLEDGVIGGRAWVPKGARGG*RGGT GAAAGSSSDSEETLRGPRGS*GPEGYRGRRARH PSLGSTTGHCGGGRGAEGTGTDPAAPAARLNVD GLLVYFPYDYIYPEQFSYMRELKRSWSPGDALRH REDSIPVGPDHGIPESISAGGDQTHLLLKNCAR D*EGD*RASKVAQLL*EAGGREAAVSGTGSELP QKLVYSP*GSLPDPWHSCPRNLMPMGVRCPSPLA STTWMT*RPWGGARAGAHTSLLDTQSCMPMWWF IATTTSWTPRLQTWCPRNWPARPSWSSTRPTTL TTSASTP*ASTSPAGPLTGARATWRPCRRRCS

Frame 1

LSCSS*SRTPGFDQYSDNLGRRRRTKF**MRSS LSALSLPCFLFDWPSTILSDASRTASPESQPPS LFRAHAQYPDWLCPSGLTGRLANGLVI*VERALE DVHDPASR*IFMREAGRPRCTVRPAP*SEKGAG GRAR*RARGVLKMGSSVGAPGSPRGRGEGEGVG RGQPQGAAVIARRH*GGPEAPEDLRVTGGAGPVT LLWARRPGTVEAGEGLRGRELTQQPLPPGSTWT GSWSTSRTTTSTPSSSPTCGSSNGHGVLEMPSG TGKTVSLLALIMAYQRAYPLEVTKLIYCSRTVPE IEKVIEELRKLLNFYEKQEGEKLPFLGLALSSR KNLCIHPEAASLTPGTLVPGI*CPWA*GAPPRW HLQPG*PEGPGAAPGLVPILPCSILNPACQCGGL *LPLPPGPQDCRPGVQGTGPQGRRGLRRGPQH* QRLHRLHERQPHPPDP*PVPGQPGDPAEDGAQ

Frame 2

YLAQADLELLGSINTQTILAGAGGPNSSE*DRV SRLFPFHVFFLIGPRRSSVTPPAPPHPRVSRPR FSVRTRSIPIGSALAD*RAG*PMVS*YRWSEPS RMSTTRPLAEYS*GRRVDPAAQSGRRHEVRRGLG VALASGRGGS*RWGHRWARLGPQGGEGRVKGWD GGSRREQQ**RGDTEGAPRLLRT*GLPGAPGPS PFSGLDDRALWRRERG*GDGN*PSSPCRQAQRGR APGLLPVRLHLPRAVLLHAGAQTVMESWRCPQA PGRQYPCWP*SWHTREHIRWR*PNSSTAQELCQ RLRR*LKSFESCSTSMRSRRARSCRFWDWL*APA KTCVFTLRQPP*PLALLSQEFDAHGREVPLPAG IYNLDDLKALGRRQGWCPYFLARYSILHANVVV YSYHYLLDPKIADLVSKELARKAVVVFDEAHNID NVCIDSMSVNLTRRTLDRCQGNLETLQKTVL

Sequence ERCC2.dAug05

ERCC2.u.f.11 (forward primer)
ERCC2.u.r.11 (reverse primer)
Exon skipping and alternative donor or acceptor

Frame 0

Frame 1

Frame 2

Sequence ERCC2.cAug05

GCTATCTTGCTCAAGCTGATCTCGAACTCCTGGGTTCGATCAATACTCAGACAATCTTGGCAGGCGCAGGAGGACCAAATTCTAGTGAATGAGATCGAGT CTCTCGGCTCTTTCCCTTCCATGTTTTCTTTTTGATTGGCCCTCGACGATCCTCAGTGACGCCTCCCGCACCGCCTCACCCGAGAGTCAGCCGCCCTCGC TTTTCCGTGCGCACGCGCAGTATCCCGATTGGCTCTGCCCTAGCGGATTGACGGGCAGGTTAGCCAATGGTCTCGTAATATAGGTGGAGCGAGCCCTCGA GGATGTCCACGACCCGGCCTCTCGCTGAATATTCATGAGGGAGGCGGGTCGACCCCGCTGCACAGTCCGGCCGGCGCCATGAAGTGAGAAGGGGGCTGGG GGTCGCGCTCGCTAGCGGGCGCGGGGGGTCTTGAAGATGGGGTCATCGGCTCAACGTGGACGGGCTCCTGGTCTACTTCCCGTACGACTACATCTACCCC GAGCAGTTCTCCTACATGCGGGAGCTCAAACGCACGCTGGACGCCAAGGGTCATGGAGTCCTGGAGATGCCCTCAGGCACCGGGAAGACAGTATCCCTGT TGGCCCTGATCATGGCATACCAGAGAGCATATCCGCTGGAGGTGACCAAACTCATCTACTGCTCAAGAACTGTGCCAGAGATTGAGAAGGTGATTGAAGA GCTTCGAAAGTTGCTCAACTTCTATGAGAAGCAGGAGGGCGAGAAGCTGCCGTTTCTGGGACTGGCTCTGAGCTCCCGCAAAAACTTGTGTATTCACCCT GAGGTGACACCCCTGCGCTTTGGGAAGGACGTCGATGGGAAATGCCACAGCCTCACAGCCTCCTATGTGCGGGCGCAGTACCAGCATGACACCAGCCTGC CCCACTGCCGATTCTATGAGGAATTTGATGCCCATGGGCGTGAGGTGCCCCTCCCCGCTGGCATCTACAACCTGGATGACCTGAAGGCCCTGGGGCGGCG CCAGGGCTGGTGCCCATACTTCCTTGCTCGATACTCAATCCTGCATGCCAATGTGGTGGTTTATAGCTACCACTACCTCCTGGACCCCAAGATTGCAGAC CTGGTGTCCAAGGAACTGGCCCGCAAGGCCGTCGTGGTCTTCGACGAGGCCCACAACATTGACAACGTCTGCATCGACTCCATGAGCGTCAACCTCACCC GCCGGACCCTTGACCGGTGCCAGGGCAACCTGGAGACCCTGCAGAAGACGGTGCTCAGGATCAAAGAGACAGACGAGCAGCGCCTGCGGGACGAGTACCG GCGTCTGGTGGAGGGGCTGCGGGAGGCCAGCGCCGCCCGGGAGACGGACGCCCACCTGGCCAACCCCGTGCTGCCCGACGAAGTGCTGCAGGTGCCTGGC TCCATCCGCACGGCCGAGCATTTCCTGGGCTTCCTGAGGCGGCTGCTGGAGTACGTGAAGTGGCGGCTGCGTGTGCAGCATGTGGTGCAGGAGAGCCCGC CCGCCTTCCTGAGCGGCCTGGCCCAGCGCGTGTGCATCCAGCGCAAGCCCCTCAGATTCTGTGCTGAACGCCTCCGGTCCCTGCTGCATACTCTGGAGAT CACCGACCTTGCTGACTTCTCCCCGCTCACCCTCCTTGCTAACTTTGCCACCCTTGTCAGCACCTACGCCAAAGGCTTCACCATCATCATCGAGCCCTTT GACGACAGAACCCCGACCATTGCCAACCCCATCCTGCACTTCAGCTGCATGGACGCCTCGCTGGCCATCAAACCCGTATTTGAGCGTTTCCAGTCTGTCA TCATCACATCTGGGACACTGTCCCCGCTGGACATCTACCCCAAGATCCTGGACTTCCACCCCGTCACCATGGCAACCTTCACCATGACGCTGGCACGGGT CTGCCTCTGCCCTATGATCATCGGCCGTGGCAATGACCAGGTGGCCATCAGCTCCAAATTTGAGACCCGGGAGGATATTGCTGTGATCCGGAACTATGGG AACCTCCTGCTGGAGATGTCCGCTGTGGTCCCTGATGGCATCGTGGCCTTCTTCACCAGCTACCAGTACATGGAGAGCACCGTGGCCTCCTGGTATGAGC AGGGGATCCTTGAGAACATCCAGAGGAACAAGCTGCTCTTTATTGAGACCCAGGATGGTGCCGAAACCAGTGTCGCCCTGGAGAAGTACCAGGAGGCCTG CGAGAATGGCCGCGGGGCCATCCTGCTGTCAGTGGCCCGGGGCAAAGTGTCCGAGGGAATCGACTTTGTGCACCACTACGGGCGGGCCGTCATCATGTTT GGCGTCCCCTACGTCTACACACAGAGCCGCATTCTCAAGGCGCGGCTGGAATACCTGCGGGACCAGTTCCAGATTCGTGAGAATGACTTTCTTACCTTCG ATGCCATGCGCCACGCGGCCCAGTGTGTGGGTCGGGCCATCAGGGGCAAGACGGACTACGGCCTCATGGTCTTTGCCGACAAGGTCGGTTTGCCCGTGGG GACAAGCGGGGGAAGCTGCCCCGCTGGATCCAGGAGCACCTCACAGATGCCAACCTCAACCTGACCGTGGACGAGGGTGTCCAGGTGGCCAAGTACTTCC TGCGGCAGATGGCACAGCCCTTCCACCGGGAGGATCAGCTGGGCCTGTCCCTGCTCAGCCTGGAGCAGCTAGAATCAGAGGAGACGCTGAAGAGGATAGA GCAGATTGCTCAGCAGCTCTGAGTGGGGCGGGTGGGGCCATAAACGGTTCCTGGTGACTCCTGAGTCTTGCCTGGCCCTGGTTCCCAGCGGCGGTGGTGC TAGAAGGTCTTATGAAGTCAGGTGACATTTCTCACTGTCACGTCCACAGCCTTTAATCGCAGGAGAAGGCAGCTATCCACCAGGTACCCAGAGGCAAGGG GGGGCCAGGAGATGATAGACCCCCTCTCACCCCACCAGCCCATCCCTCCTGCACTGTTCCCAAGAAGCTGCGGCCAGCGCAGTTCACTGGACGTTAGTGG GGGCTCAGGTCCTGGGTGCTGGCGCTGAGGGTCCGAGGGGCCTTGTCCAGGTGCCAGCTGGGAAACTGGAGACAGGCACAGGGGATGGTTGGAGGGGAGG TGAGGGGCAAGTGTTACATCCACCCTCCAGCTCCAGGTCCAGCCTGGCAGCCAACTATCCAACCCCCTCGCCTCTACCCCAAGTAAAACCCATCTTTGCT CCTGTCCTGGGTCTCTTCTGACACCAACCCACCACCTGCTGACTCCACCACTGGCCCGACCGCCCTGCCTTTGCCTCATCTCTGCACCTACCCACATGGC CTCCTCTATTTTTGACCCAGATGTCCCCCTCACCTGAGTCCCAGGAGCCCTTGGAGCATCCACGTTCAGTGTGTTGAGTGACATGGCTCTCTTCATTCTG CAAAGAGGGCAGCAGGGAGGAAATGAGTGAATCCAGGAGTGGCCCCCCTCCACGAGGGACCTTTCCAGCACAGGGTTTGATCTGTGTGTATCACAGGGGA GATGGGAGCCATGGAAGGTTCTTGAGCAAGATGGGGGTGGGGGTGGGGTGGGCTGTGGATTCTGCTGATGTCAGGGCTCTTTGCCGACCTGATCAACACT CACCCGGCTGCTCCTGCCGCCGCCTCGCCTCGGAGCCGGGAGACCAGCTTCTCACTTCCTCGCCTGATAGACTCACGGATCTTGGAGAGTGAGCTGCTGC GGCGAAGGGCCTGGGGAGGGGCCGGGGATGCTGCTCAGTCCCCCAGGCCCATGCAGTCCCCACGCCCACCCCAGGAGCCGAAGCACAGCCCATCCTCACC TGTTCTGCGTCACCAGCTGTGCCTGTGTTGGGGGCACCTAGGGGGAAAGATGGGGGACACTGAGATGGGGACATAGAGAAGCCACCAGGAGGGAAGACAG ACAGGGGCTAGGGGCTCACCGAGAGGGGCGGGTAGGTCCTCCTTGTGGAGGATTTCTTTGTACAGCTCTTCCGCTTGTTGATACTTGTTCTGTTTCAGGT AGGCTGAGGCCTGCGGAGAAGGGAGTGTCTGGGAACTTTTCGGACCCCAGTTCCCAACACTGCATCCAGTCCCGCCTTTTTTTTTTTTTTTGTAGAGACA GGGGTCTTGGTATGTTGCCCAGGCTGGTCTTGAACTCCTAGCCTCAAGTGATCCTCCTGCCTTGGCCTCCCAAAGTGTTGGGGTTACAAGCGTGAACCAC CACGTCTGGCCCCACTTTCTAATGTGCTGACCTGGCCGTATCCTTCCCCTGCCTAAAGCCTTGCCCTGGGGCAGAGTGGGTTTGGAGCTCGGCCTTACTT CCTGCCCTGGGGATGGAGGACAGGCAGCCTCCTGTTTCTGAGCCTGTCTGCTGTACTTTGGGCTGGTGGCATGGCCTCGGTGCCTCAGTCTTCCCACACG CAAAGTGGAGCGACAGCTCCCACCTCATAGGGACGCTATAAGAACTAGCGATGTGGGGACCGGGCTAAGAGGGGAGCCGGCCACAGTGAGCTCAATAAAT GTCTGCTGCTGCCACCTGTGCCTGCCAGCTTCCGCTTCACTGGCTGTCCTCTGGCTGCCGTGGACTTACGCGTT

ERCC2.u.f.11 (forward primer)
ERCC2.u.r.11 (reverse primer)
Exon skipping and alternative donor or acceptor

Frame 0

AILLKLISNSWVRSILRQSWQAQEDQILVNEIE SLGSFPSMFSF*LALDDPQ*RLPHRLTRESAALA FPCARAVSRLALP*RIDGQVSQWSRNIGGASPR GCPRPGLSLNIHEGGGSTPLHSPAGAMK*EGGW GSRSLAGAGGLEDGVIGSTWTGSWSTSRTTTSTP SSSPTCGSSNARWTPRVMESWRCPQAPGRQYPC WP*SWHTREHIRWR*PNSSTAQELCQRLRR*LK SFESCSTSMRSRRARSCRFWDWL*APAKTCVFTL R*HPCALGRTSMGNATASQPPMCGRSTSMTPAC PTADSMRNLMPMGVRCPSPLASTTWMT*RPWGG ARAGAHTSLLDTQSCMPMWWFIATTTSWTPRLQT WCPRNWPARPSWSSTRPTTLTTSASTP*ASTSP AGPLTGARATWRPCRRRCSGSKRQTSSACGTST GVWWRGCGRPAPPGRRTPTWPTPCCPTKCCRCLA PSARPSISWAS*GGCWST*SGGCVCSMWCRRAR PPS*AAWPSACASSASPSDSVLNASGPCCILWR SPTLLTSPRSPSLLTLPPLSAPTPKASPSSSSPL TTEPRPLPTPSCTSAAWTPRWPSNPYLSVSSLS SSHLGHCPRWTSTPRSWTSTPSPWQPSP*RWHG SASAL*SSAVAMTRWPSAPNLRPGRILL*SGTMG TSCWRCPLWSLMASWPSSPATSTWRAPWPPGMS RGSLRTSRGTSCSLLRPRMVPKPVSPWRSTRRP ARMAAGPSCCQWPGAKCPRESTLCTTTGGPSSCL ASPTSTHRAAFSRRGWNTCGTSSRFVRMTFLPS MPCATRPSVWVGPSGARRTTASWSLPTRSVCPW GQAGEAAPLDPGAPHRCQPQPDRGRGCPGGQVLP AADGTALPPGGSAGPVPAQPGAARIRGDAEEDR ADCSAALSGAGGAINGSW*LLSLAWPWFPAAVV LEGLMKSGDISHCHVHSL*SQEKAAIHQVPRGKG GPGDDRPPLTPPAHPSCTVPKKLRPAQFTGR*W GLRSWVLALRVRGALSRCQLGNWRQAQGMVGGE VRGKCYIHPPAPGPAWQPTIQPPRLYPK*NPSLL LSWVSSDTNPPPADSTTGPTALPLPHLCTYPHG LLYF*PRCPPHLSPRSPWSIHVQCVE*HGSLHS AKRAAGRK*VNPGVAPLHEGPFQHRV*SVCITGE MGAMEGS*ARWGWGWGGLWILLMSGLFADLINT HPAAPAAASPRSRETSFSLPRLIDSRILESELL RRRAWGGAGDAAQSPRPMQSPRPPQEPKHSPSSP VLRHQLCLCWGHLGGKMGDTEMGT*RSHQEGRQ TGARGSPRGAGRSSLWRISLYSSSAC*YLFCFR *AEACGEGSVWELFGPQFPTLHPVPPFFFFL*RQ GSWYVAQAGLELLASSDPPALASQSVGVTSVNH HVWPHFLMC*PGRILPLPKALPWGRVGLELGLT SCPGDGGQAASCF*ACLLYFGLVAWPRCLSLPTR KVERQLPPHRDAIRTSDVGTGLRGEPATVSSIN VCCCHLCLPASASLAVLWLPWTYA

Frame 1

LSCSS*SRTPGFDQYSDNLGRRRRTKF**MRSS LSALSLPCFLFDWPSTILSDASRTASPESQPPS LFRAHAQYPDWLCPSGLTGRLANGLVI*VERALE DVHDPASR*IFMREAGRPRCTVRPAP*SEKGAG GRAR*RARGVLKMGSSAQRGRAPGLLPVRLHLP RAVLLHAGAQTHAGRQGSWSPGDALRHREDSIPV GPDHGIPESISAGGDQTHLLLKNCARD*EGD*R ASKVAQLL*EAGGREAAVSGTGSELPQKLVYSP *GDTPALWEGRRWEMPQPHSLLCAGAVPA*HQPA PLPIL*GI*CPWA*GAPPRWHLQPG*PEGPGAA PGLVPILPCSILNPACQCGGL*LPLPPGPQDCR PGVQGTGPQGRRGLRRGPQH*QRLHRLHERQPHP PDP*PVPGQPGDPAEDGAQDQRDRRAAPAGRVP ASGGGAAGGQRRPGDGRPPGQPRAARRSAAGAW LHPHGRAFPGLPEAAAGVREVAAACAACGAGEPA RLPERPGPARVHPAQAPQILC*TPPVPAAYSGD HRPC*LLPAHPPC*LCHPCQHLRQRLHHHHRAL *RQNPDHCQPHPALQLHGRLAGHQTRI*AFPVCH HHIWDTVPAGHLPQDPGLPPRHHGNLHHDAGTG LPLPYDHRPWQ*PGGHQLQI*DPGGYCCDPELW EPPAGDVRCGP*WHRGLLHQLPVHGEHRGLLV*A GDP*EHPEEQAALY*DPGWCRNQCRPGEVPGGL REWPRGHPAVSGPGQSVRGNRLCAPLRAGRHHV WRPLRLHTEPHSQGAAGIPAGPVPDS*E*LSYLR CHAPRGPVCGSGHQGQDGLRPHGLCRQGRFARG DKRGKLPRWIQEHLTDANLNLTVDEGVQVAKYF LRQMAQPFHREDQLGLSLLSLEQLESEETLKRIE QIAQQL*VGRVGP*TVPGDS*VLPGPGSQRRWC *KVL*SQVTFLTVTSTAFNRRRRQLSTRYPEAR GGQEMIDPLSPHQPIPPALFPRSCGQRSSLDVSG GSGPGCWR*GSEGPCPGASWETGDRHRGWLEGR *GASVTSTLQLQVQPGSQLSNPLASTPSKTHLC SCPGSLLTPTHHLLTPPLARPPCLCLISAPTHMA SSIFDPDVPLT*VPGALGASTFSVLSDMALFIL QRGQQGGNE*IQEWPPSTRDLSSTGFDLCVSQG RWEPWKVLEQDGGGGGVGCGFC*CQGSLPT*STL TRLLLPPPRLGAGRPASHFLA**THGSWRVSCC GEGPGEGPGMLLSPPGPCSPHAHPRSRSTAHPH LFCVTSCACVGGT*GERWGTLRWGHREATRREDR QGLGAHREGRVGPPCGGFLCTALPLVDTCSVSG RLRPAEKGVSGNFSDPSSQHCIQSRLFFFFCRD RGLGMLPRLVLNS*PQVILLPWPPKVLGLQA*TT TSGPTF*CADLAVSFPCLKPCPGAEWVWSSALL PALGMEDRQPPVSEPVCCTLGWWHGLGASVFPH AKWSDSSHLIGTL*ELAMWGPG*EGSRPQ*AQ*M SAAATCACQLPLHWLSSGCRGLTR

Frame 2

YLAQADLELLGSINTQTILAGAGGPNSSE*DRV SRLFPFHVFFLIGPRRSSVTPPAPPHPRVSRPR FSVRTRSIPIGSALAD*RAG*PMVS*YRWSEPS RMSTTRPLAEYS*GRRVDPAAQSGRRHEVRRGLG VALASGRGGS*RWGHRLNVDGLLVYFPYDYIYP EQFSYMRELKRTLDAKGHGVLEMPSGTGKTVSL LALIMAYQRAYPLEVTKLIYCSRTVPEIEKVIEE LRKLLNFYEKQEGEKLPFLGLALSSRKNLCIHP EVTPLRFGKDVDGKCHSLTASYVRAQYQHDTSL PHCRFYEEFDAHGREVPLPAGIYNLDDLKALGRR QGWCPYFLARYSILHANVVVYSYHYLLDPKIAD LVSKELARKAVVVFDEAHNIDNVCIDSMSVNLT RRTLDRCQGNLETLQKTVLRIKETDEQRLRDEYR RLVEGLREASAARETDAHLANPVLPDEVLQVPG SIRTAEHFLGFLRRLLEYVKWRLRVQHVVQESP PAFLSGLAQRVCIQRKPLRFCAERLRSLLHTLEI TDLADFSPLTLLANFATLVSTYAKGFTIIIEPF DDRTPTIANPILHFSCMDASLAIKPVFERFQSV IITSGTLSPLDIYPKILDFHPVTMATFTMTLARV CLCPMIIGRGNDQVAISSKFETREDIAVIRNYG NLLLEMSAVVPDGIVAFFTSYQYMESTVASWYE QGILENIQRNKLLFIETQDGAETSVALEKYQEAC ENGRGAILLSVARGKVSEGIDFVHHYGRAVIMF GVPYVYTQSRILKARLEYLRDQFQIRENDFLTF DAMRHAAQCVGRAIRGKTDYGLMVFADKVGLPVG TSGGSCPAGSRSTSQMPTST*PWTRVSRWPSTS CGRWHSPSTGRISWACPCSAWSS*NQRRR*RG* SRLLSSSEWGGWGHKRFLVTPESCLALVPSGGGA RRSYEVR*HFSLSRPQPLIAGEGSYPPGTQRQG GARR**TPSHPTSPSLLHCSQEAAASAVHWTLV GAQVLGAGAEGPRGLVQVPAGKLETGTGDGWRGG EGQVLHPPSSSRSSLAANYPTPSPLPQVKPIFA PVLGLF*HQPTTC*LHHWPDRPAFASSLHLPTW PPLFLTQMSPSPESQEPLEHPRSVC*VTWLSSFC KEGSREEMSESRSGPPPRGTFPAQGLICVYHRG DGSHGRFLSKMGVGVGWAVDSADVRALCRPDQH SPGCSCRRLASEPGDQLLTSSPDRLTDLGE*AAA AKGLGRGRGCCSVPQAHAVPTPTPGAEAQPILT CSASPAVPVLGAPRGKDGGH*DGDIEKPPGGKT DRG*GLTERGG*VLLVEDFFVQLFRLLILVLFQV G*GLRRRECLGTFRTPVPNTASSPAFFFFFVET GVLVCCPGWS*TPSLK*SSCLGLPKCWGYKREP PRLAPLSNVLTWPYPSPA*SLALGQSGFGARPYF LPWGWRTGSLLFLSLSAVLWAGGMASVPQSSHT QSGATAPTS*GRYKN*RCGDRAKRGAGHSELNK CLLLPPVPASFRFTGCPLAAVDLRV

Sequence ERCC2.bAug05

GCTATCTTGCTCAAGCTGATCTCGAACTCCTGGGTTCGATCAATACTCAGACAATCTTGGCAGGCGCAGGAGGACCAAATTCTAGTGAATGAGATCGAGT CTCTCGGCTCTTTCCCTTCCATGTTTTCTTTTTGATTGGCCCTCGACGATCCTCAGTGACGCCTCCCGCACCGCCTCACCCGAGAGTCAGCCGCCCTCGC TTTTCCGTGCGCACGCGCAGTATCCCGATTGGCTCTGCCCTAGCGGATTGACGGGCAGGTTAGCCAATGGTCTCGTAATATAGGTGGAGCGAGCCCTCGA GGATGTCCACGACCCGGCCTCTCGCTGAATATTCATGAGGGAGGCGGGTCGACCCCGCTGCACAGTCCGGCCGGCGCCATGAAGCTCAACGTGGACGGGC TCCTGGTCTACTTCCCGTACGACTACATCTACCCCGAGCAGTTCTCCTACATGCGGGAGCTCAAACGCACGCTGGACGCCAAGGGTCATGGAGTCCTGGA GATGCCCTCAGGCACCGGGAAGACAGTATCCCTGTTGGCCCTGATCATGGCATACCAGAGAGCATATCCGCTGGAGGTGACCAAACTCATCTACTGCTCA AGAACTGTGCCAGAGATTGAGAAGGTGATTGAAGAGCTTCGAAAGTTGCTCAACTTCTATGAGAAGCAGGAGGGCGAGAAGCTGCCGTTTCTGGGACTGG CTCTGAGCTCCCGCAAAAACTTGTGTATTCACCCTGAGGTGACACCCCTGCGCTTTGGGAAGGACGTCGATGGGAAATGCCACAGCCTCACAGCCTCCTA TGTGCGGGCGCAGTACCAGCATGACACCAGCCTGCCCCACTGCCGATTCTATGAGGAATTTGATGCCCATGGGCGTGAGGTGCCCCTCCCCGCTGGCATC TACAACCTGGATGACCTGAAGGCCCTGGGGCGGCGCCAGGGCTGGTGCCCATACTTCCTTGCTCGATACTCAATCCTGCATGCCAATGTGGTGGTTTATA GCTACCACTACCTCCTGGACCCCAAGATTGCAGACCTGGTGTCCAAGGAACTGGCCCGCAAGGCCGTCGTGGTCTTCGACGAGGCCCACAACATTGACAA CGTCTGCATCGACTCCATGAGCGTCAACCTCACCCGCCGGACCCTTGACCGGTGCCAGGGCAACCTGGAGACCCTGCAGAAGACGGTGCTCAGGATCAAA GAGACAGACGAGCAGCGCCTGCGGGACGAGTACCGGCGTCTGGTGGAGGGGCTGCGGGAGGCCAGCGCCGCCCGGGAGACGGACGCCCACCTGGCCAACC CCGTGCTGCCCGACGAAGTGCTGCAGGAGGCAGTGCCTGGCTCCATCCGCACGGCCGAGCATTTCCTGGGCTTCCTGAGGCGGCTGCTGGAGTACGTGAA GTGGCGGCTGCGTGTGCAGCATGTGGTGCAGGAGAGCCCGCCCGCCTTCCTGAGCGGCCTGGCCCAGCGCGTGTGCATCCAGCGCAAGCCCCTCAGATTC TGTGCTGAACGCCTCCGGTCCCTGCTGCATACTCTGGAGATCACCGACCTTGCTGACTTCTCCCCGCTCACCCTCCTTGCTAACTTTGCCACCCTTGTCA GCACCTACGCCAAAGGCTTCACCATCATCATCGAGCCCTTTGACGACAGAACCCCGACCATTGCCAACCCCATCCTGCACTTCAGCTGCATGGACGCCTC GCTGGCCATCAAACCCGTATTTGAGCGTTTCCAGTCTGTCATCATCACATCTGGGACACTGTCCCCGCTGGACATCTACCCCAAGATCCTGGACTTCCAC CCCGTCACCATGGCAACCTTCACCATGACGCTGGCACGGGTCTGCCTCTGCCCTATGATCATCGGCCGTGGCAATGACCAGGTGGCCATCAGCTCCAAAT TTGAGACCCGGGAGGATATTGCTGTGATCCGGAACTATGGGAACCTCCTGCTGGAGATGTCCGCTGTGGTCCCTGATGGCATCGTGGCCTTCTTCACCAG CTACCAGTACATGGAGAGCACCGTGGCCTCCTGGTATGAGCAGGGGATCCTTGAGAACATCCAGAGGAACAAGCTGCTCTTTATTGAGACCCAGGATGGT GCCGAAACCAGTGTCGCCCTGGAGAAGTACCAGGAGGCCTGCGAGAATGGCCGCGGGGCCATCCTGCTGTCAGTGGCCCGGGGCAAAGTGTCCGAGGGAA TCGACTTTGTGCACCACTACGGGCGGGCCGTCATCATGTTTGGCGTCCCCTACGTCTACACACAGAGCCGCATTCTCAAGGCGCGGCTGGAATACCTGCG GGACCAGTTCCAGATTCGTGAGAATGACTTTCTTACCTTCGATGCCATGCGCCACGCGGCCCAGTGTGTGGGTCGGGCCATCAGGGGCAAGACGGACTAC GGCCTCATGGTCTTTGCCGACAAGCGGTTTGCCCGTGGGGACAAGCGGGGGAAGCTGCCCCGCTGGATCCAGGAGCACCTCACAGATGCCAACCTCAACC TGACCGTGGACGAGGGTGTCCAGGTGGCCAAGTACTTCCTGCGGCAGATGGCACAGCCCTTCCACCGGGAGGATCAGCTGGGCCTGTCCCTGCTCAGCCT GGAGCAGCTAGAATCAGAGGAGACGCTGAAGAGGATAGAGCAGATTGCTCAGCAGCTCTGAGTGGGGCGGGTGGGGCCATAAACGGTTCCTGGTGACTCC TGAGTCTTGCCTGGCCCTGGTTCCCAGCGGCGGTGGTGCTAGAAGGTCTTATGAAGTCAGGTGACATTTCTCACTGTCACGTCCACAGCCTTTAATCGCA GGAGAAGGCAGCTATCCACCAGGTACCCAGAGGCAAGGGGGGGCCAGGAGATGATAGACCCCCTCTCACCCCACCAGCCCATCCCTCCTGCACTGTTCCC AAGAAGCTGCGGCCAGCGCAGTTCACTGGACGTTAGTGGGGGCTCAGGTCCTGGGTGCTGGCGCTGAGGGTCCGAGGGGCCTTGTCCAGGTGCCAGCTGG GAAACTGGAGACAGGCACAGGGGATGGTTGGAGGGGAGGTGAGGGGCAAGTGTTACATCCACCCTCCAGCTCCAGGTCCAGCCTGGCAGCCAACTATCCA ACCCCCTCGCCTCTACCCCAAGTAAAACCCATCTTTGCTCCTGTCCTGGGTCTCTTCTGACACCAACCCACCACCTGCTGACTCCACCACTGGCCCGACC GCCCTGCCTTTGCCTCATCTCTGCACCTACCCACATGGCCTCCTCTATTTTTGACCCAGATGTCCCCCTCACCTGAGTCCCAGGAGCCCTTGGAGCATCC ACGTTCAGTGTGTTGAGTGACATGGCTCTCTTCATTCTGCAAAGAGGGCAGCAGGGAGGAAATGAGTGAATCCAGGAGTGGCCCCCCTCCACGAGGGACC TTTCCAGCACAGGGTTTGATCTGTGTGTATCACAGGGGAGATGGGAGCCATGGAAGGTTCTTGAGCAAGATGGGGGTGGGGGTGGGGTGGGCTGTGGATT CTGCTGATGTCAGGGCTCTTTGCCGACCTGATCAACACTCACCCGGCTGCTCCTGCCGCCGCCTCGCCTCGGAGCCGGGAGACCAGCTTCTCACTTCCTC GCCTGATAGACTCACGGATCTTGGAGAGTGAGCTGCTGCGGCGAAGGGCCTGGGGAGGGGCCGGGGATGCTGCTCAGTCCCCCAGGCCCATGCAGTCCCC ACGCCCACCCCAGGAGCCGAAGCACAGCCCATCCTCACCTGTTCTGCGTCACCAGCTGTGCCTGTGTTGGGGGCACCTAGGGGGAAAGATGGGGGACACT GAGATGGGGACATAGAGAAGCCACCAGGAGGGAAGACAGACAGGGGCTAGGGGCTCACCGAGAGGGGCGGGTAGGTCCTCCTTGTGGAGGATTTCTTTGT ACAGCTCTTCCGCTTGTTGATACTTGTTCTGTTTCAGGTAGGCTGAGGCCTGCGGAGAAGGGAGTGTCTGGGAACTTTTCGGACCCCAGTTCCCAACACT GCATCCAGTCCCGCCTTTTTTTTTTTTTTTGTAGAGACAGGGGTCTTGGTATGTTGCCCAGGCTGGTCTTGAACTCCTAGCCTCAAGTGATCCTCCTGCC TTGGCCTCCCAAAGTGTTGGGGTTACAAGCGTGAACCACCACGTCTGGCCCCACTTTCTAATGTGCTGACCTGGCCGTATCCTTCCCCTGCCTAAAGCCT TGCCCTGGGGCAGAGTGGGTTTGGAGCTCGGCCTTACTTCCTGCCCTGGGGATGGAGGACAGGCAGCCTCCTGTTTCTGAGCCTGTCTGCTGTACTTTGG GCTGGTGGCATGGCCTCGGTGCCTCAGTCTTCCCACACGCAAAGTGGAGCGACAGCTCCCACCTCATAGGGACGCTATAAGAACTAGCGATGTGGGGACC GGGCTAAGAGGGGAGCCGGCCACAGTGAGCTCAATAAATGTCTGCTGCTGCCACCTGTGCCTGCCAGCTTCCGCTTCACTGGCTGTCCTCTGGCTGCCGT GGACTTACGCGTT

ERCC2.u.f.11 (forward primer)
ERCC2.u.r.11 (reverse primer)
Exon skipping and alternative donor or acceptor

Frame 0

AILLKLISNSWVRSILRQSWQAQEDQILVNEIE SLGSFPSMFSF*LALDDPQ*RLPHRLTRESAALA FPCARAVSRLALP*RIDGQVSQWSRNIGGASPR GCPRPGLSLNIHEGGGSTPLHSPAGAMKLNVDG LLVYFPYDYIYPEQFSYMRELKRTLDAKGHGVLE MPSGTGKTVSLLALIMAYQRAYPLEVTKLIYCS RTVPEIEKVIEELRKLLNFYEKQEGEKLPFLGL ALSSRKNLCIHPEVTPLRFGKDVDGKCHSLTASY VRAQYQHDTSLPHCRFYEEFDAHGREVPLPAGI YNLDDLKALGRRQGWCPYFLARYSILHANVVVY SYHYLLDPKIADLVSKELARKAVVVFDEAHNIDN VCIDSMSVNLTRRTLDRCQGNLETLQKTVLRIK ETDEQRLRDEYRRLVEGLREASAARETDAHLAN PVLPDEVLQEAVPGSIRTAEHFLGFLRRLLEYVK WRLRVQHVVQESPPAFLSGLAQRVCIQRKPLRF CAERLRSLLHTLEITDLADFSPLTLLANFATLV STYAKGFTIIIEPFDDRTPTIANPILHFSCMDAS LAIKPVFERFQSVIITSGTLSPLDIYPKILDFH PVTMATFTMTLARVCLCPMIIGRGNDQVAISSK FETREDIAVIRNYGNLLLEMSAVVPDGIVAFFTS YQYMESTVASWYEQGILENIQRNKLLFIETQDG AETSVALEKYQEACENGRGAILLSVARGKVSEG IDFVHHYGRAVIMFGVPYVYTQSRILKARLEYLR DQFQIRENDFLTFDAMRHAAQCVGRAIRGKTDY GLMVFADKRFARGDKRGKLPRWIQEHLTDANLN LTVDEGVQVAKYFLRQMAQPFHREDQLGLSLLSL EQLESEETLKRIEQIAQQL*VGRVGP*TVPGDS *VLPGPGSQRRWC*KVL*SQVTFLTVTSTAFNR RRRQLSTRYPEARGGQEMIDPLSPHQPIPPALFP RSCGQRSSLDVSGGSGPGCWR*GSEGPCPGASW ETGDRHRGWLEGR*GASVTSTLQLQVQPGSQLS NPLASTPSKTHLCSCPGSLLTPTHHLLTPPLARP PCLCLISAPTHMASSIFDPDVPLT*VPGALGAS TFSVLSDMALFILQRGQQGGNE*IQEWPPSTRD LSSTGFDLCVSQGRWEPWKVLEQDGGGGGVGCGF C*CQGSLPT*STLTRLLLPPPRLGAGRPASHFL A**THGSWRVSCCGEGPGEGPGMLLSPPGPCSP HAHPRSRSTAHPHLFCVTSCACVGGT*GERWGTL RWGHREATRREDRQGLGAHREGRVGPPCGGFLC TALPLVDTCSVSGRLRPAEKGVSGNFSDPSSQH CIQSRLFFFFCRDRGLGMLPRLVLNS*PQVILLP WPPKVLGLQA*TTTSGPTF*CADLAVSFPCLKP CPGAEWVWSSALLPALGMEDRQPPVSEPVCCTL GWWHGLGASVFPHAKWSDSSHLIGTL*ELAMWGP G*EGSRPQ*AQ*MSAAATCACQLPLHWLSSGCR GLTR

Frame 1

LSCSS*SRTPGFDQYSDNLGRRRRTKF**MRSS LSALSLPCFLFDWPSTILSDASRTASPESQPPS LFRAHAQYPDWLCPSGLTGRLANGLVI*VERALE DVHDPASR*IFMREAGRPRCTVRPAP*SSTWTG SWSTSRTTTSTPSSSPTCGSSNARWTPRVMESW RCPQAPGRQYPCWP*SWHTREHIRWR*PNSSTAQ ELCQRLRR*LKSFESCSTSMRSRRARSCRFWDW L*APAKTCVFTLR*HPCALGRTSMGNATASQPP MCGRSTSMTPACPTADSMRNLMPMGVRCPSPLAS TTWMT*RPWGGARAGAHTSLLDTQSCMPMWWFI ATTTSWTPRLQTWCPRNWPARPSWSSTRPTTLT TSASTP*ASTSPAGPLTGARATWRPCRRRCSGSK RQTSSACGTSTGVWWRGCGRPAPPGRRTPTWPT PCCPTKCCRRQCLAPSARPSISWAS*GGCWST* SGGCVCSMWCRRARPPS*AAWPSACASSASPSDS VLNASGPCCILWRSPTLLTSPRSPSLLTLPPLS APTPKASPSSSSPLTTEPRPLPTPSCTSAAWTP RWPSNPYLSVSSLSSSHLGHCPRWTSTPRSWTST PSPWQPSP*RWHGSASAL*SSAVAMTRWPSAPN LRPGRILL*SGTMGTSCWRCPLWSLMASWPSSP ATSTWRAPWPPGMSRGSLRTSRGTSCSLLRPRMV PKPVSPWRSTRRPARMAAGPSCCQWPGAKCPRE STLCTTTGGPSSCLASPTSTHRAAFSRRGWNTC GTSSRFVRMTFLPSMPCATRPSVWVGPSGARRTT ASWSLPTSGLPVGTSGGSCPAGSRSTSQMPTST *PWTRVSRWPSTSCGRWHSPSTGRISWACPCSA WSS*NQRRR*RG*SRLLSSSEWGGWGHKRFLVTP ESCLALVPSGGGARRSYEVR*HFSLSRPQPLIA GEGSYPPGTQRQGGARR**TPSHPTSPSLLHCS QEAAASAVHWTLVGAQVLGAGAEGPRGLVQVPAG KLETGTGDGWRGGEGQVLHPPSSSRSSLAANYP TPSPLPQVKPIFAPVLGLF*HQPTTC*LHHWPD RPAFASSLHLPTWPPLFLTQMSPSPESQEPLEHP RSVC*VTWLSSFCKEGSREEMSESRSGPPPRGT FPAQGLICVYHRGDGSHGRFLSKMGVGVGWAVD SADVRALCRPDQHSPGCSCRRLASEPGDQLLTSS PDRLTDLGE*AAAAKGLGRGRGCCSVPQAHAVP TPTPGAEAQPILTCSASPAVPVLGAPRGKDGGH *DGDIEKPPGGKTDRG*GLTERGG*VLLVEDFFV QLFRLLILVLFQVG*GLRRRECLGTFRTPVPNT ASSPAFFFFFVETGVLVCCPGWS*TPSLK*SSC LGLPKCWGYKREPPRLAPLSNVLTWPYPSPA*SL ALGQSGFGARPYFLPWGWRTGSLLFLSLSAVLW AGGMASVPQSSHTQSGATAPTS*GRYKN*RCGD RAKRGAGHSELNKCLLLPPVPASFRFTGCPLAAV DLRV

Frame 2

YLAQADLELLGSINTQTILAGAGGPNSSE*DRV SRLFPFHVFFLIGPRRSSVTPPAPPHPRVSRPR FSVRTRSIPIGSALAD*RAG*PMVS*YRWSEPS RMSTTRPLAEYS*GRRVDPAAQSGRRHEAQRGRA PGLLPVRLHLPRAVLLHAGAQTHAGRQGSWSPG DALRHREDSIPVGPDHGIPESISAGGDQTHLLL KNCARD*EGD*RASKVAQLL*EAGGREAAVSGTG SELPQKLVYSP*GDTPALWEGRRWEMPQPHSLL CAGAVPA*HQPAPLPIL*GI*CPWA*GAPPRWH LQPG*PEGPGAAPGLVPILPCSILNPACQCGGL* LPLPPGPQDCRPGVQGTGPQGRRGLRRGPQH*Q RLHRLHERQPHPPDP*PVPGQPGDPAEDGAQDQ RDRRAAPAGRVPASGGGAAGGQRRPGDGRPPGQP RAARRSAAGGSAWLHPHGRAFPGLPEAAAGVRE VAAACAACGAGEPARLPERPGPARVHPAQAPQI LC*TPPVPAAYSGDHRPC*LLPAHPPC*LCHPCQ HLRQRLHHHHRAL*RQNPDHCQPHPALQLHGRL AGHQTRI*AFPVCHHHIWDTVPAGHLPQDPGLP PRHHGNLHHDAGTGLPLPYDHRPWQ*PGGHQLQI *DPGGYCCDPELWEPPAGDVRCGP*WHRGLLHQ LPVHGEHRGLLV*AGDP*EHPEEQAALY*DPGW CRNQCRPGEVPGGLREWPRGHPAVSGPGQSVRGN RLCAPLRAGRHHVWRPLRLHTEPHSQGAAGIPA GPVPDS*E*LSYLRCHAPRGPVCGSGHQGQDGL RPHGLCRQAVCPWGQAGEAAPLDPGAPHRCQPQP DRGRGCPGGQVLPAADGTALPPGGSAGPVPAQP GAARIRGDAEEDRADCSAALSGAGGAINGSW*L LSLAWPWFPAAVVLEGLMKSGDISHCHVHSL*SQ EKAAIHQVPRGKGGPGDDRPPLTPPAHPSCTVP KKLRPAQFTGR*WGLRSWVLALRVRGALSRCQL GNWRQAQGMVGGEVRGKCYIHPPAPGPAWQPTIQ PPRLYPK*NPSLLLSWVSSDTNPPPADSTTGPT ALPLPHLCTYPHGLLYF*PRCPPHLSPRSPWSI HVQCVE*HGSLHSAKRAAGRK*VNPGVAPLHEGP FQHRV*SVCITGEMGAMEGS*ARWGWGWGGLWI LLMSGLFADLINTHPAAPAAASPRSRETSFSLP RLIDSRILESELLRRRAWGGAGDAAQSPRPMQSP RPPQEPKHSPSSPVLRHQLCLCWGHLGGKMGDT EMGT*RSHQEGRQTGARGSPRGAGRSSLWRISL YSSSAC*YLFCFR*AEACGEGSVWELFGPQFPTL HPVPPFFFFL*RQGSWYVAQAGLELLASSDPPA LASQSVGVTSVNHHVWPHFLMC*PGRILPLPKA LPWGRVGLELGLTSCPGDGGQAASCF*ACLLYFG LVAWPRCLSLPTRKVERQLPPHRDAIRTSDVGT GLRGEPATVSSINVCCCHLCLPASASLAVLWLP WTYA

Sequence ERCC2.aAug05

GCTATCTTGCTCAAGCTGATCTCGAACTCCTGGGTTCGATCAATACTCAGACAATCTTGGCAGGCGCAGGAGGACCAAATTCTAGTGAATGAGATCGAGT CTCTCGGCTCTTTCCCTTCCATGTTTTCTTTTTGATTGGCCCTCGACGATCCTCAGTGACGCCTCCCGCACCGCCTCACCCGAGAGTCAGCCGCCCTCGC TTTTCCGTGCGCACGCGCAGTATCCCGATTGGCTCTGCCCTAGCGGATTGACGGGCAGGTTAGCCAATGGTCTCGTAATATAGGTGGAGCGAGCCCTCGA GGATGTCCACGACCCGGCCTCTCGCTGAATATTCATGAGGGAGGCGGGTCGACCCCGCTGCACAGTCCGGCCGGCGCCATGAAGTGAGAAGGGGGCTGGG GGTCGCGCTCGCTAGCGGGCGCGGGGGGTCTTGAAGATGGGGTCATCGGTGGGCGCGCCTGGGTCCCCAAGGGGGCGAGGGGAGGGTGAAGGGGTGGGAC GGGGGCAGCCGCAGGGAGCAGCAGTGATAGCGAGGAGACACTGAGGGGGCCCCGAGGCTCCTGAGGACCTGAGGGTTACCGGGGGCGCCGGGCCCGTCAC CCTTCTCTGGGCTCGACGACCGGGCACTGTGGAGGCGGGAGAGGGGCTGAGGGGACGGGAACTGACCCAGCAGCCCCTGCCGCCAGGCTCAACGTGGACG GGCTCCTGGTCTACTTCCCGTACGACTACATCTACCCCGAGCAGTTCTCCTACATGCGGGAGCTCAAACGCACGCTGGACGCCAAGGGTCATGGAGTCCT GGAGATGCCCTCAGGCACCGGGAAGACAGTATCCCTGTTGGCCCTGATCATGGCATACCAGAGAGCATATCCGCTGGAGGTGACCAAACTCATCTACTGC TCAAGAACTGTGCCAGAGATTGAGAAGGTGATTGAAGAGCTTCGAAAGTTGCTCAACTTCTATGAGAAGCAGGAGGGCGAGAAGCTGCCGTTTCTGGGAC TGGCTCTGAGCTCCCGCAAAAACTTGTGTATTCACCCTGAGGTGACACCCCTGCGCTTTGGGAAGGACGTCGATGGGAAATGCCACAGCCTCACAGCCTC CTATGTGCGGGCGCAGTACCAGCATGACACCAGCCTGCCCCACTGCCGATTCTATGAGGAATTTGATGCCCATGGGCGTGAGGTGCCCCTCCCCGCTGGC ATCTACAACCTGGATGACCTGAAGGCCCTGGGGCGGCGCCAGGGCTGGTGCCCATACTTCCTTGCTCGATACTCAATCCTGCATGCCAATGTGGTGGTTT ATAGCTACCACTACCTCCTGGACCCCAAGATTGCAGACCTGGTGTCCAAGGAACTGGCCCGCAAGGCCGTCGTGGTCTTCGACGAGGCCCACAACATTGA CAACGTCTGCATCGACTCCATGAGCGTCAACCTCACCCGCCGGACCCTTGACCGGTGCCAGGGCAACCTGGAGACCCTGCAGAAGACGGTGCTCAGGATC AAAGAGACAGACGAGCAGCGCCTGCGGGACGAGTACCGGCGTCTGGTGGAGGGGCTGCGGGAGGCCAGCGCCGCCCGGGAGACGGACGCCCACCTGGCCA ACCCCGTGCTGCCCGACGAAGTGCTGCAGGAGGCAGTGCCTGGCTCCATCCGCACGGCCGAGCATTTCCTGGGCTTCCTGAGGCGGCTGCTGGAGTACGT GAAGTGGCGGCTGCGTGTGCAGCATGTGGTGCAGGAGAGCCCGCCCGCCTTCCTGAGCGGCCTGGCCCAGCGCGTGTGCATCCAGCGCAAGCCCCTCAGA TTCTGTGCTGAACGCCTCCGGTCCCTGCTGCATACTCTGGAGATCACCGACCTTGCTGACTTCTCCCCGCTCACCCTCCTTGCTAACTTTGCCACCCTTG TCAGCACCTACGCCAAAGGCTTCACCATCATCATCGAGCCCTTTGACGACAGAACCCCGACCATTGCCAACCCCATCCTGCACTTCAGCTGCATGGACGC CTCGCTGGCCATCAAACCCGTATTTGAGCGTTTCCAGTCTGTCATCATCACATCTGGGACACTGTCCCCGCTGGACATCTACCCCAAGATCCTGGACTTC CACCCCGTCACCATGGCAACCTTCACCATGACGCTGGCACGGGTCTGCCTCTGCCCTATGATCATCGGCCGTGGCAATGACCAGGTGGCCATCAGCTCCA AATTTGAGACCCGGGAGGATATTGCTGTGATCCGGAACTATGGGAACCTCCTGCTGGAGATGTCCGCTGTGGTCCCTGATGGCATCGTGGCCTTCTTCAC CAGCTACCAGTACATGGAGAGCACCGTGGCCTCCTGGTATGAGCAGGGGATCCTTGAGAACATCCAGAGGAACAAGCTGCTCTTTATTGAGACCCAGGAT GGTGCCGAAACCAGTGTCGCCCTGGAGAAGTACCAGGAGGCCTGCGAGAATGGCCGCGGGGCCATCCTGCTGTCAGTGGCCCGGGGCAAAGTGTCCGAGG GAATCGACTTTGTGCACCACTACGGGCGGGCCGTCATCATGTTTGGCGTCCCCTACGTCTACACACAGAGCCGCATTCTCAAGGCGCGGCTGGAATACCT GCGGGACCAGTTCCAGATTCGTGAGAATGACTTTCTTACCTTCGATGCCATGCGCCACGCGGCCCAGTGTGTGGGTCGGGCCATCAGGGGCAAGACGGAC TACGGCCTCATGGTCTTTGCCGACAAGCGGTTTGCCCGTGGGGACAAGCGGGGGAAGCTGCCCCGCTGGATCCAGGAGCACCTCACAGATGCCAACCTCA ACCTGACCGTGGACGAGGGTGTCCAGGTGGCCAAGTACTTCCTGCGGCAGATGGCACAGCCCTTCCACCGGGAGGATCAGCTGGGCCTGTCCCTGCTCAG CCTGGAGCAGCTAGAATCAGAGGAGACGCTGAAGAGGATAGAGCAGATTGCTCAGCAGCTCTGAGTGGGGCGGGTGGGGCCATAAACGGTTCCTGGTGAC TCCTGAGTCTTGCCTGGCCCTGGTTCCCAGCGGCGGTGGTGCTAGAAGGTCTTATGAAGTCAGGTGACATTTCTCACTGTCACGTCCACAGCCTTTAATC GCAGGAGAAGGCAGCTATCCACCAGGTACCCAGAGGCAAGGGGGGGCCAGGAGATGATAGACCCCCTCTCACCCCACCAGCCCATCCCTCCTGCACTGTT CCCAAGAAGCTGCGGCCAGCGCAGTTCACTGGACGTTAGTGGGGGCTCAGGTCCTGGGTGCTGGCGCTGAGGGTCCGAGGGGCCTTGTCCAGGTGCCAGC TGGGAAACTGGAGACAGGCACAGGGGATGGTTGGAGGGGAGGTGAGGGGCAAGTGTTACATCCACCCTCCAGCTCCAGGTCCAGCCTGGCAGCCAACTAT CCAACCCCCTCGCCTCTACCCCAAGTAAAACCCATCTTTGCTCCTGTCCTGGGTCTCTTCTGACACCAACCCACCACCTGCTGACTCCACCACTGGCCCG ACCGCCCTGCCTTTGCCTCATCTCTGCACCTACCCACATGGCCTCCTCTATTTTTGACCCAGATGTCCCCCTCACCTGAGTCCCAGGAGCCCTTGGAGCA TCCACGTTCAGTGTGTTGAGTGACATGGCTCTCTTCATTCTGCAAAGAGGGCAGCAGGGAGGAAATGAGTGAATCCAGGAGTGGCCCCCCTCCACGAGGG ACCTTTCCAGCACAGGGTTTGATCTGTGTGTATCACAGGGGAGATGGGAGCCATGGAAGGTTCTTGAGCAAGATGGGGGTGGGGGTGGGGTGGGCTGTGG ATTCTGCTGATGTCAGGGCTCTTTGCCGACCTGATCAACACTCACCCGGCTGCTCCTGCCGCCGCCTCGCCTCGGAGCCGGGAGACCAGCTTCTCACTTC CTCGCCTGATAGACTCACGGATCTTGGAGAGTGAGCTGCTGCGGCGAAGGGCCTGGGGAGGGGCCGGGGATGCTGCTCAGTCCCCCAGGCCCATGCAGTC CCCACGCCCACCCCAGGAGCCGAAGCACAGCCCATCCTCACCTGTTCTGCGTCACCAGCTGTGCCTGTGTTGGGGGCACCTAGGGGGAAAGATGGGGGAC ACTGAGATGGGGACATAGAGAAGCCACCAGGAGGGAAGACAGACAGGGGCTAGGGGCTCACCGAGAGGGGCGGGTAGGTCCTCCTTGTGGAGGATTTCTT TGTACAGCTCTTCCGCTTGTTGATACTTGTTCTGTTTCAGGTAGGCTGAGGCCTGCGGAGAAGGGAGTGTCTGGGAACTTTTCGGACCCCAGTTCCCAAC ACTGCATCCAGTCCCGCCTTTTTTTTTTTTTTTGTAGAGACAGGGGTCTTGGTATGTTGCCCAGGCTGGTCTTGAACTCCTAGCCTCAAGTGATCCTCCT GCCTTGGCCTCCCAAAGTGTTGGGGTTACAAGCGTGAACCACCACGTCTGGCCCCACTTTCTAATGTGCTGACCTGGCCGTATCCTTCCCCTGCCTAAAG CCTTGCCCTGGGGCAGAGTGGGTTTGGAGCTCGGCCTTACTTCCTGCCCTGGGGATGGAGGACAGGCAGCCTCCTGTTTCTGAGCCTGTCTGCTGTACTT TGGGCTGGTGGCATGGCCTCGGTGCCTCAGTCTTCCCACACGCAAAGTGGAGCGACAGCTCCCACCTCATAGGGACGCTATAAGAACTAGCGATGTGGGG ACCGGGCTAAGAGGGGAGCCGGCCACAGTGAGCTCAATAAATGTCTGCTGCTGCCACCTGTGCCTGCCAGCTTCCGCTTCACTGGCTGTCCTCTGGCTGC CGTGGACTTACGCGTT

ERCC2.u.f.11 (forward primer)
ERCC2.u.r.11 (reverse primer)
Exon skipping and alternative donor or acceptor

Frame 0

AILLKLISNSWVRSILRQSWQAQEDQILVNEIE SLGSFPSMFSF*LALDDPQ*RLPHRLTRESAALA FPCARAVSRLALP*RIDGQVSQWSRNIGGASPR GCPRPGLSLNIHEGGGSTPLHSPAGAMK*EGGW GSRSLAGAGGLEDGVIGGRAWVPKGARGG*RGGT GAAAGSSSDSEETLRGPRGS*GPEGYRGRRARH PSLGSTTGHCGGGRGAEGTGTDPAAPAARLNVD GLLVYFPYDYIYPEQFSYMRELKRTLDAKGHGVL EMPSGTGKTVSLLALIMAYQRAYPLEVTKLIYC SRTVPEIEKVIEELRKLLNFYEKQEGEKLPFLG LALSSRKNLCIHPEVTPLRFGKDVDGKCHSLTAS YVRAQYQHDTSLPHCRFYEEFDAHGREVPLPAG IYNLDDLKALGRRQGWCPYFLARYSILHANVVV YSYHYLLDPKIADLVSKELARKAVVVFDEAHNID NVCIDSMSVNLTRRTLDRCQGNLETLQKTVLRI KETDEQRLRDEYRRLVEGLREASAARETDAHLA NPVLPDEVLQEAVPGSIRTAEHFLGFLRRLLEYV KWRLRVQHVVQESPPAFLSGLAQRVCIQRKPLR FCAERLRSLLHTLEITDLADFSPLTLLANFATL VSTYAKGFTIIIEPFDDRTPTIANPILHFSCMDA SLAIKPVFERFQSVIITSGTLSPLDIYPKILDF HPVTMATFTMTLARVCLCPMIIGRGNDQVAISS KFETREDIAVIRNYGNLLLEMSAVVPDGIVAFFT SYQYMESTVASWYEQGILENIQRNKLLFIETQD GAETSVALEKYQEACENGRGAILLSVARGKVSE GIDFVHHYGRAVIMFGVPYVYTQSRILKARLEYL RDQFQIRENDFLTFDAMRHAAQCVGRAIRGKTD YGLMVFADKRFARGDKRGKLPRWIQEHLTDANL NLTVDEGVQVAKYFLRQMAQPFHREDQLGLSLLS LEQLESEETLKRIEQIAQQL*VGRVGP*TVPGD S*VLPGPGSQRRWC*KVL*SQVTFLTVTSTAFN RRRRQLSTRYPEARGGQEMIDPLSPHQPIPPALF PRSCGQRSSLDVSGGSGPGCWR*GSEGPCPGAS WETGDRHRGWLEGR*GASVTSTLQLQVQPGSQL SNPLASTPSKTHLCSCPGSLLTPTHHLLTPPLAR PPCLCLISAPTHMASSIFDPDVPLT*VPGALGA STFSVLSDMALFILQRGQQGGNE*IQEWPPSTR DLSSTGFDLCVSQGRWEPWKVLEQDGGGGGVGCG FC*CQGSLPT*STLTRLLLPPPRLGAGRPASHF LA**THGSWRVSCCGEGPGEGPGMLLSPPGPCS PHAHPRSRSTAHPHLFCVTSCACVGGT*GERWGT LRWGHREATRREDRQGLGAHREGRVGPPCGGFL CTALPLVDTCSVSGRLRPAEKGVSGNFSDPSSQ HCIQSRLFFFFCRDRGLGMLPRLVLNS*PQVILL PWPPKVLGLQA*TTTSGPTF*CADLAVSFPCLK PCPGAEWVWSSALLPALGMEDRQPPVSEPVCCT LGWWHGLGASVFPHAKWSDSSHLIGTL*ELAMWG PG*EGSRPQ*AQ*MSAAATCACQLPLHWLSSGC RGLTR

Frame 1

LSCSS*SRTPGFDQYSDNLGRRRRTKF**MRSS LSALSLPCFLFDWPSTILSDASRTASPESQPPS LFRAHAQYPDWLCPSGLTGRLANGLVI*VERALE DVHDPASR*IFMREAGRPRCTVRPAP*SEKGAG GRAR*RARGVLKMGSSVGAPGSPRGRGEGEGVG RGQPQGAAVIARRH*GGPEAPEDLRVTGGAGPVT LLWARRPGTVEAGEGLRGRELTQQPLPPGSTWT GSWSTSRTTTSTPSSSPTCGSSNARWTPRVMES WRCPQAPGRQYPCWP*SWHTREHIRWR*PNSSTA QELCQRLRR*LKSFESCSTSMRSRRARSCRFWD WL*APAKTCVFTLR*HPCALGRTSMGNATASQP PMCGRSTSMTPACPTADSMRNLMPMGVRCPSPLA STTWMT*RPWGGARAGAHTSLLDTQSCMPMWWF IATTTSWTPRLQTWCPRNWPARPSWSSTRPTTL TTSASTP*ASTSPAGPLTGARATWRPCRRRCSGS KRQTSSACGTSTGVWWRGCGRPAPPGRRTPTWP TPCCPTKCCRRQCLAPSARPSISWAS*GGCWST *SGGCVCSMWCRRARPPS*AAWPSACASSASPSD SVLNASGPCCILWRSPTLLTSPRSPSLLTLPPL SAPTPKASPSSSSPLTTEPRPLPTPSCTSAAWT PRWPSNPYLSVSSLSSSHLGHCPRWTSTPRSWTS TPSPWQPSP*RWHGSASAL*SSAVAMTRWPSAP NLRPGRILL*SGTMGTSCWRCPLWSLMASWPSS PATSTWRAPWPPGMSRGSLRTSRGTSCSLLRPRM VPKPVSPWRSTRRPARMAAGPSCCQWPGAKCPR ESTLCTTTGGPSSCLASPTSTHRAAFSRRGWNT CGTSSRFVRMTFLPSMPCATRPSVWVGPSGARRT TASWSLPTSGLPVGTSGGSCPAGSRSTSQMPTS T*PWTRVSRWPSTSCGRWHSPSTGRISWACPCS AWSS*NQRRR*RG*SRLLSSSEWGGWGHKRFLVT PESCLALVPSGGGARRSYEVR*HFSLSRPQPLI AGEGSYPPGTQRQGGARR**TPSHPTSPSLLHC SQEAAASAVHWTLVGAQVLGAGAEGPRGLVQVPA GKLETGTGDGWRGGEGQVLHPPSSSRSSLAANY PTPSPLPQVKPIFAPVLGLF*HQPTTC*LHHWP DRPAFASSLHLPTWPPLFLTQMSPSPESQEPLEH PRSVC*VTWLSSFCKEGSREEMSESRSGPPPRG TFPAQGLICVYHRGDGSHGRFLSKMGVGVGWAV DSADVRALCRPDQHSPGCSCRRLASEPGDQLLTS SPDRLTDLGE*AAAAKGLGRGRGCCSVPQAHAV PTPTPGAEAQPILTCSASPAVPVLGAPRGKDGG H*DGDIEKPPGGKTDRG*GLTERGG*VLLVEDFF VQLFRLLILVLFQVG*GLRRRECLGTFRTPVPN TASSPAFFFFFVETGVLVCCPGWS*TPSLK*SS CLGLPKCWGYKREPPRLAPLSNVLTWPYPSPA*S LALGQSGFGARPYFLPWGWRTGSLLFLSLSAVL WAGGMASVPQSSHTQSGATAPTS*GRYKN*RCG DRAKRGAGHSELNKCLLLPPVPASFRFTGCPLAA VDLRV

Frame 2

YLAQADLELLGSINTQTILAGAGGPNSSE*DRV SRLFPFHVFFLIGPRRSSVTPPAPPHPRVSRPR FSVRTRSIPIGSALAD*RAG*PMVS*YRWSEPS RMSTTRPLAEYS*GRRVDPAAQSGRRHEVRRGLG VALASGRGGS*RWGHRWARLGPQGGEGRVKGWD GGSRREQQ**RGDTEGAPRLLRT*GLPGAPGPS PFSGLDDRALWRRERG*GDGN*PSSPCRQAQRGR APGLLPVRLHLPRAVLLHAGAQTHAGRQGSWSP GDALRHREDSIPVGPDHGIPESISAGGDQTHLL LKNCARD*EGD*RASKVAQLL*EAGGREAAVSGT GSELPQKLVYSP*GDTPALWEGRRWEMPQPHSL LCAGAVPA*HQPAPLPIL*GI*CPWA*GAPPRW HLQPG*PEGPGAAPGLVPILPCSILNPACQCGGL *LPLPPGPQDCRPGVQGTGPQGRRGLRRGPQH* QRLHRLHERQPHPPDP*PVPGQPGDPAEDGAQD QRDRRAAPAGRVPASGGGAAGGQRRPGDGRPPGQ PRAARRSAAGGSAWLHPHGRAFPGLPEAAAGVR EVAAACAACGAGEPARLPERPGPARVHPAQAPQ ILC*TPPVPAAYSGDHRPC*LLPAHPPC*LCHPC QHLRQRLHHHHRAL*RQNPDHCQPHPALQLHGR LAGHQTRI*AFPVCHHHIWDTVPAGHLPQDPGL PPRHHGNLHHDAGTGLPLPYDHRPWQ*PGGHQLQ I*DPGGYCCDPELWEPPAGDVRCGP*WHRGLLH QLPVHGEHRGLLV*AGDP*EHPEEQAALY*DPG WCRNQCRPGEVPGGLREWPRGHPAVSGPGQSVRG NRLCAPLRAGRHHVWRPLRLHTEPHSQGAAGIP AGPVPDS*E*LSYLRCHAPRGPVCGSGHQGQDG LRPHGLCRQAVCPWGQAGEAAPLDPGAPHRCQPQ PDRGRGCPGGQVLPAADGTALPPGGSAGPVPAQ PGAARIRGDAEEDRADCSAALSGAGGAINGSW* LLSLAWPWFPAAVVLEGLMKSGDISHCHVHSL*S QEKAAIHQVPRGKGGPGDDRPPLTPPAHPSCTV PKKLRPAQFTGR*WGLRSWVLALRVRGALSRCQ LGNWRQAQGMVGGEVRGKCYIHPPAPGPAWQPTI QPPRLYPK*NPSLLLSWVSSDTNPPPADSTTGP TALPLPHLCTYPHGLLYF*PRCPPHLSPRSPWS IHVQCVE*HGSLHSAKRAAGRK*VNPGVAPLHEG PFQHRV*SVCITGEMGAMEGS*ARWGWGWGGLW ILLMSGLFADLINTHPAAPAAASPRSRETSFSL PRLIDSRILESELLRRRAWGGAGDAAQSPRPMQS PRPPQEPKHSPSSPVLRHQLCLCWGHLGGKMGD TEMGT*RSHQEGRQTGARGSPRGAGRSSLWRIS LYSSSAC*YLFCFR*AEACGEGSVWELFGPQFPT LHPVPPFFFFL*RQGSWYVAQAGLELLASSDPP ALASQSVGVTSVNHHVWPHFLMC*PGRILPLPK ALPWGRVGLELGLTSCPGDGGQAASCF*ACLLYF GLVAWPRCLSLPTRKVERQLPPHRDAIRTSDVG TGLRGEPATVSSINVCCCHLCLPASASLAVLWL PWTYA