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Sequences

Sequence LIG3.cAug05

GCCTGCGCGCTGCCTCCCGCTCTAGGACCCGGATTTAAAGAGACAGGCGCTCCAACCGTCGTGGGCTGCCCGCGGCCTGTAATGAGCAAGTTCCGAGGCC
TACGGTGAGCGCCGGAGCCGGAGAGGCAGGGTTGCATGTGGGACTCTGCAGTGGCCCCTGTGAGATGGCTGAGCAACGGTTCTGTGTGGACTATGCCAAG
CGTGGCACAGCTGGCTGCAAAAAATGCAAGGAAAAGATTGTGAAGGGCGTATGCCGAATTGGCAAAGTGGTGCCCAATCCCTTCTCAGAGTCTGGGGGTG
ATATGAAAGAGTGGTACCACATTAAATGCATGTTTGAGAAACTAGAGCGGGCCCGGGCCACCACAAAAAAAATCGAGGACCTCACAGAGCTGGAAGGCTG
GGAAGAGCTGGAAGATAATGAGAAGGAACAGATAACCCAGCACATTGCAGATCTGTCTTCTAAGGCAGCAGGTACACCAAAGAAGAAAGCTGTTGTCCAG
GCTAAGTTGACAACCACTGGCCAGGTGACTTCTCCAGTGAAAGGCGCCTCATTTGTCACCAGTACCAATCCCCGGAAATTTTCTGGCTTTTCAGCCAAGC
CCAACAACTCTGGGGAAGCCCCCTCGAGCCCCACCCCTAAGAGAAGTCTGTCTTCAAGCAAATGTGACCCCAGGCATAAGGACTGTCTGCTACGGGAGTT
TCGAAAGTTATGCGCCATGGTGGCCGATAATCCTAGCTACAACACGAAGACCCAGATCATCCAGGACTTCCTTCGGAAAGGCTCAGCAGGAGATGGTTTC
CACGGTGATGTGTACCTAACAGTGAAGCTGCTGCTGCCAGGAGTCATTAAGACTGTTTACAACTTGAACGATAAGCAGATTGTGAAGCTTTTCAGTCGCA
TTTTTAACTGCAACCCAGATGATATGGCACGGGACCTAGAGCAGGTCAGAGGAACGGGAGGGAGGGTAGGCTACATTCCAGGTGGGGTTTTGCCAAGCCA
GGCACCTGCAGCCTCTTTTTTTTTTTCTGGTTGGGACACATACTGTTTTAGGAAACTGAAACCCATTTGAGCAAGCTTCCGTTATAGGGGGAGCTGAATG
TGAGGCTATAGCTCTTAATTTCTTAAGTTTCTGAGGGTGGAAAC
  • LIG3.u.f.1 (forward primer)
  • LIG3.u.r.6 (reverse primer)
  • Alternative acceptor

Frame 0

ACALPPALGPGFKETGAPTVVGCPRPVMSKFRG
LR*APEPERQGCMWDSAVAPVRWLSNGSVWTMPS
VAQLAAKNARKRL*RAYAELAKWCPIPSQSLGV
I*KSGTTLNACLRN*SGPGPPQKKSRTSQSWKA
GKSWKIMRRNR*PSTLQICLLRQQVHQRRKLLSR
LS*QPLAR*LLQ*KAPHLSPVPIPGNFLAFQPS
PTTLGKPPRAPPLREVCLQANVTPGIRTVCYGS
FESYAPWWPIILATTRRPRSSRTSFGKAQQEMVS
TVMCT*Q*SCCCQESLRLFTT*TISRL*SFSVA
FLTATQMIWHGT*SRSEEREGG*ATFQVGFCQA
RHLQPLFFFLVGTHTVLGN*NPFEQASVIGGAEC
EAIALNFLSF*GWK

Frame 1

PARCLPL*DPDLKRQALQPSWAARGL**ASSEA
YGERRSRRGRVACGTLQWPL*DG*ATVLCGLCQ
AWHSWLQKMQGKDCEGRMPNWQSGAQSLLRVWG*
YERVVPH*MHV*ETRAGPGHHKKNRGPHRAGRL
GRAGR**EGTDNPAHCRSVF*GSRYTKEESCCP
G*VDNHWPGDFSSERRLICHQYQSPEIFWLFSQA
QQLWGSPLEPHP*EKSVFKQM*PQA*GLSATGV
SKVMRHGGR*S*LQHEDPDHPGLPSERLSRRWF
PR*CVPNSEAAAARSH*DCLQLER*ADCEAFQSH
F*LQPR*YGTGPRAGQRNGREGRLHSRWGFAKP
GTCSLFFFFWLGHILF*ETETHLSKLPL*GELN
VRL*LLIS*VSEGGN

Frame 2

LRAASRSRTRI*RDRRSNRRGLPAACNEQVPRP
TVSAGAGEAGLHVGLCSGPCEMAEQRFCVDYAK
RGTAGCKKCKEKIVKGVCRIGKVVPNPFSESGG
DMKEWYHIKCMFEKLERARATTKKIEDLTELEGW
EELEDNEKEQITQHIADLSSKAAGTPKKKAVVQ
AKLTTTGQVTSPVKGASFVTSTNPRKFSGFSAK
PNNSGEAPSSPTPKRSLSSSKCDPRHKDCLLREF
RKLCAMVADNPSYNTKTQIIQDFLRKGSAGDGF
HGDVYLTVKLLLPGVIKTVYNLNDKQIVKLFSR
IFNCNPDDMARDLEQVRGTGGRVGYIPGGVLPSQ
APAASFFFSGWDTYCFRKLKPI*ASFRYRGS*M
*GYSS*FLKFLRVE

Sequence LIG3.aAug05

GCCTGCGCGCTGCCTCCCGCTCTAGGACCCGGATTTAAAGAGACAGGCGCTCCAACCGTCGTGGGCTGCCCGCGGCCTGTAATGAGCAAGTTCCGAGGCC
TACGGTGAGCGCCGGAGCCGGAGAGGCAGCTATATGTCTTTGGCTTTCAAGATCTTCTTTCCACAAACCCTCCGTGCACTCAGCCGAAAAGAACTGTGCC
TATTCCGAAAACATCACTGGCGTGATGTAAGACAATTCAGCCAGTGGTCAGAAACAGATCTGCTTCATGGACATCCCCTCTTCCTGAGAAGAAAGCCTGT
TCTATCATTCCAGGGAAGCCATCTAAGATCACGTGCCACCTACCTTGTTTTCTTGCCAG
GGTTGCATGTGGGACTCTGCAGTGGCCCCTGTGAGATGGCT
GAGCAAC
GGTTCTGTGTGGACTATGCCAAGCGTGGCACAGCTGGCTGCAAAAAATGCAAGGAAAAGATTGTGAAGGGCGTATGCCGAATTGGCAAAGTGG
TGCCCAATCCCTTCTCAGAGTCTGGGGGTGATATGAAAGAGTGGTACCACATTAAATGCATGTTTGAGAAACTAGAGCGGGCCCGGGCCACCACAAAAAA
AATCGAGGACCTCACAGAGCTGGAAGGCTGGGAAGAGCTGGAAGATAATGAGAAGGAACAGATAACCCAGCACATTGCAGATCTGTCTTCTAAGGCAGCA
GGTACACCAAAGAAGAAAGCTGTTGTCCAGGCTAAGTTGACAACCACTGGCCAGGTGACTTCTCCAGTGAAAGGCGCCTCATTTGTCACCAGTACCAATC
CCCGGAAATTTTCTGGCTTTTCAGCCAAGCCCAACAACTCTGGGGAAGCCCCCTCGAGCCCCACCCCTAAGAGAAGTCTGTCTTCAAGCAAATGTGACCC
CAGGCATAAGGACTGTCTGCTACGGGAGTTTCGAAAGTTATGCGCCATGGTGGCCGATAATCCTAGCTACAACACGAAGACCCAGATCATCCAGGACTTC
CTTCGGAAAGGCTCAGCAGGAGATGGTTTCCACGGTGATGTGTACCTAACAGTGAAGCTGCTGCTGCCAGGAGTCATTAAGACTGTTTACAACTTGAACG
ATAAGCAGATTGTGAAGCTTTTCAGTCGCATTTTTAACTGCAACCCAGATGATATGGCACGGGACCTAGAGCAGGGTGACGTGTCAGAGACAATCAGAGT
CTTCTTTGAGCAGAGCAAGTCTTTCCCCCCAGCTGCCAAGAGCCTCCTTACCATCCAGGAAGTGGATGAGTTCCTTCTGCGGCTGTCCAAGCTCACCAAG
GAGGATGAGCAGCAACAGGCCCTACAGGACATTGCCTCCAGGTGTACAGCCAATGACCTTAAATGCATCATCAGGTTGATCAAACATGATCTGAAGATGA
ACTCAGGTGCAAAACATGTGTTAGACGCCCTTGACCCCAATGCCTATGAAGCCTTCAAAGCCTCGCGCAACCTGCAGGATGTGGTGGAGCGGGTCCTTCA
CAACGCGCAGGAGGTGGAGAAGGAGCCGGGCCAGAGACGAGCTCTGAGCGTCCAGGCCTCGCTGATGACACCTGTGCAGCCCATGTTGGCGGAGGCCTGC
AAGTCCGTTGAGTATGCAATGAAGAAATGTCCCAATGGCATGTTCTCTGAGATCAAGTACGATGGAGAGCGAGTCCAGGTGCATAAGAATGGAGACCACT
TCAGCTACTTCAGCCGCAGTCTCAAGCCCGTCCTTCCTCACAAGGTGGCCCACTTTAAGGACTACATTCCCCAGGCTTTTCCTGGGGGCCACAGCATGAT
CTTGGATTCTGAAGTGCTTCTGATTGACAACAAGACAGGCAAACCACTGCCCTTTGGGACTCTGGGAGTACACAAGAAAGCAGCCTTCCAGGATGCTAAT
GTCTGCCTGTTTGTTTTTGATTGTATCTACTTTAATGATGTCAGCTTGATGGACAGACCTCTGTGTGAGCGGCGGAAGTTTCTTCATGACAACATGGTTG
AAATTCCAAACCGGATCATGTTCTCAGAAATGAAGCGAGTCACAAAAGCTTTGGACTTGGCTGACATGATAACCCGGGTGATCCAGGAGGGATTGGAGGG
GCTGGTGCTGAAGGATGTGAAGGGTACATATGAGCCTGGGAAGCGGCACTGGCTGAAAGTGAAGAAAGACTATTTGAACGAGGGGGCCATGGCCGACACA
GCTGACCTGGTGGTCCTTGGAGCCTTCTATGGGCAAGGGAGCAAAGGCGGCATGATGTCAATCTTCCTCATGGGCTGCTACGACCCTGGCAGCCAGAAGT
GGTGCACAGTCACCAAGTGTGCAGGAGGCCATGATGATGCCACGCTTGCCCGCCTGCAGAATGAACTAGACATGGTGAAGATCAGCAAGGACCCCAGCAA
AATACCCAGCTGGTTGAAGGTCAACAAGATCTACTATCCTGACTTCATCGTCCCAGACCCAAAGAAAGCTGCCGTGTGGGAGATCACAGGGGCTGAATTC
TCCAAATCGGAGGCTCATACAGCTGACGGGATCTCCATCCGATTCCCTCGCTGCACCCGAATCCGAGATGATAAGGACTGGAAATCTGCCACTAACCTTC
CCCAACTCAAGGAACTGTACCAGTTGTCCAAGGAGAAGGCAGACTTCACTGTAGTGGCTGGAGATGAGGGGAGCTCCACTACAGGGGGTAGCAGTGAAGA
GAATAAGGGTCCCTCAGGGTCTGCTGTGTCCCGCAAGGCCCCCAGCAAGCCCTCAGCCAGTACCAAGAAAGCAGAAGGGAAGCTGAGTAACTCCAACAGC
AAAGATGGCAACATGCAGACTGCAAAGCCTTCCGCTATGAAGGTGGGGGAGAAGCTGGCCACAAAGTCTTCTCCAGTGAAAGTAGGGGAGAAGCGGAAAG
CTGCTGATGAGACGCTGTGCCAAACAAAGGTATTGCTGGACATCTTCACTGGGGTGCGGCTTTACTTGCCACCCTCCACACCAGACTTCAGCCGTCTCAG
ACGCTACTTTGTGGCATTCGACGGGGACCTGGTACAGGAATTTGATATGACTTCAGCCACGCACGTGCTGGGTAGCAGGGACAAGAACCCTGCGGCCCAG
CAGGTCTCCCCAGAGTGGATTTGGGCATGTATCCGGAAACGGAGACTGGTAGCTCCCTGCTAGGTTTGCTGTCTTCCCTCTCCCTCAGGCCATACTCTCC
TTTACCATACTACTGGACTGGACTCAGGCTGGAGGCAGATAGACACAGTATAGGGGGAATGGGCTTGCTTCTCCCAAACCCACCAGTTCTCCACTGTCTC
TTCTGGACCAGGAATTAGTTGCTGTGGGTGCCACAGCTGAAGTCAGTTTGTCTTGCTGGTTTAAATAGATCTTTCAGAGCTGGGTGCTGGGTTTGCCATC
TTTTTGTTTTCTTTGAAAAGCAGCTTAGTTACCCTTTTTATAAATAAAATATCTTGCAGTTATCTTTGTCCTTTCCCCACCTACACCCCCAATAATTTCC
CTAGAGATTAAGGAGTAAAGGCTGGGCTATGGCAGCTCTGTCCACAAAGCCTTCTCTCCCATCCTTGCCTGTTCCTTTGTACTTCCAGGCTCATTTTAAA
GTTGTATTTAAAGGACTGCCCTCGGAAATGCTTCTGTTTAGCGGAACTTGTATTCAGCCTGACACGCTTTGCCAGGAACAAACCTCATGTGAAAGAAAAC
AAAATGAATTTTTTTACTTTCTTCTCTGTGTTCTCCTATTACATGGTGAGGATCCCCAGTTTGAAGGGAGGGGACTAGGGTCAGGTAGGAAAATGGGGCC
TTTATGAAGAAAGGGGAGGTTATTTGGGCCACTCCTCTGGAGGGATAGAGGGCTCCAGGAAGATCTGCATCCCCAAAACCTGGAAACAAGACTGTTCTTT
AAGAATAAAAATCCACATGGGGCTTGAGGCCAAGAACAGCCCTTGTTGCCACCAACATGGAGACTTTGTACCATATCCCATTCTTAGTGCTCGAGTGTTC
CAACCTGAAGTTGAAGAAGCCACCCTGGCTGCACATGCCATCAGCCATGACTGTGTATGCTCTGGTGGTGGTGTCTTACTTCCTCATGACTGGAGGAATA
ATTATATGATATTGTTGAACCCCCAAGTATTGGCTGATGAACGTGGGCATCAGAGGCCAGTAGCTTTCTTGGCCTACAAAGTAAATGGACAGTATATTAT
GGAAGGATTTGCATCCAGCTTCCTGTTTACAATGGGAGGTTTAGGTTTCATAATGCTGGACCAGTCGAATGCACCAAATATCCCAAAACTCAATCGATT
  • LIG3.u.f.1 (forward primer)
  • LIG3.u.r.6 (reverse primer)
  • Alternative acceptor

Frame 0

ACALPPALGPGFKETGAPTVVGCPRPVMSKFRG
LR*APEPERQLYVFGFQDLLSTNPPCTQPKRTVP
IPKTSLA*CKTIQPVVRNRSASWTSPLPEKKAC
SIIPGKPSKITCHLPCFLAR
VACGTLQWPL*DG
*AT
VLCGLCQAWHSWLQKMQGKDCEGRMPNWQSG
AQSLLRVWG*YERVVPH*MHV*ETRAGPGHHKK
NRGPHRAGRLGRAGR**EGTDNPAHCRSVF*GS
RYTKEESCCPG*VDNHWPGDFSSERRLICHQYQS
PEIFWLFSQAQQLWGSPLEPHP*EKSVFKQM*P
QA*GLSATGVSKVMRHGGR*S*LQHEDPDHPGL
PSERLSRRWFPR*CVPNSEAAAARSH*DCLQLER
*ADCEAFQSHF*LQPR*YGTGPRAG*RVRDNQS
LL*AEQVFPPSCQEPPYHPGSG*VPSAAVQAHQ
GG*AATGPTGHCLQVYSQ*P*MHHQVDQT*SEDE
LRCKTCVRRP*PQCL*SLQSLAQPAGCGGAGPS
QRAGGGEGAGPETSSERPGLADDTCAAHVGGGL
QVR*VCNEEMSQWHVL*DQVRWRASPGA*EWRPL
QLLQPQSQARPSSQGGPL*GLHSPGFSWGPQHD
LGF*SASD*QQDRQTTALWDSGSTQESSLPGC*
CLPVCF*LYLL**CQLDGQTSV*AAEVSS*QHG*
NSKPDHVLRNEASHKSFGLG*HDNPGDPGGIGG
AGAEGCEGYI*AWEAALAESEERLFERGGHGRH
S*PGGPWSLLWAREQRRHDVNLPHGLLRPWQPEV
VHSHQVCRRP**CHACPPAE*TRHGEDQQGPQQ
NTQLVEGQQDLLS*LHRPRPKESCRVGDHRG*I
LQIGGSYS*RDLHPIPSLHPNPR**GLEICH*PS
PTQGTVPVVQGEGRLHCSGWR*GELHYRG*Q*R
E*GSLRVCCVPQGPQQALSQYQESRREAE*LQQ
QRWQHADCKAFRYEGGGEAGHKVFSSESRGEAES
C**DAVPNKGIAGHLHWGAALLATLHTRLQPSQ
TLLCGIRRGPGTGI*YDFSHARAG*QGQEPCGP
AGLPRVDLGMYPETETGSSLLGLLSSLSLRPYSP
LPYYWTGLRLEADRHSIGGMGLLLPNPPVLHCL
FWTRN*LLWVPQLKSVCLAGLNRSFRAGCWVCH
LFVFFEKQLSYPFYK*NILQLSLSFPHLHPQ*FP
*RLRSKGWAMAALSTKPSLPSLPVPLYFQAHFK
VVFKGLPSEMLLFSGTCIQPDTLCQEQTSCERK
QNEFFYFLLCVLLLHGEDPQFEGRGLGSGRKMGP
L*RKGRLFGPLLWRDRGLQEDLHPQNLETRLFF
KNKNPHGA*GQEQPLLPPTWRLCTISHS*CSSV
PT*S*RSHPGCTCHQP*LCMLWWWCLTSS*LEE*
LYDIVEPPSIG**TWASEASSFLGLQSKWTVYY
GRICIQLPVYNGRFRFHNAGPVECTKYPKTQSI

Frame 1

PARCLPL*DPDLKRQALQPSWAARGL**ASSEA
YGERRSRRGSYMSLAFKIFFPQTLRALSRKELC
LFRKHHWRDVRQFSQWSETDLLHGHPLFLRRKPV
LSFQGSHLRSRATYLVFLP
GLHVGLCSGPCEMA
EQ
RFCVDYAKRGTAGCKKCKEKIVKGVCRIGKV
VPNPFSESGGDMKEWYHIKCMFEKLERARATTKK
IEDLTELEGWEELEDNEKEQITQHIADLSSKAA
GTPKKKAVVQAKLTTTGQVTSPVKGASFVTSTN
PRKFSGFSAKPNNSGEAPSSPTPKRSLSSSKCDP
RHKDCLLREFRKLCAMVADNPSYNTKTQIIQDF
LRKGSAGDGFHGDVYLTVKLLLPGVIKTVYNLN
DKQIVKLFSRIFNCNPDDMARDLEQGDVSETIRV
FFEQSKSFPPAAKSLLTIQEVDEFLLRLSKLTK
EDEQQQALQDIASRCTANDLKCIIRLIKHDLKM
NSGAKHVLDALDPNAYEAFKASRNLQDVVERVLH
NAQEVEKEPGQRRALSVQASLMTPVQPMLAEAC
KSVEYAMKKCPNGMFSEIKYDGERVQVHKNGDH
FSYFSRSLKPVLPHKVAHFKDYIPQAFPGGHSMI
LDSEVLLIDNKTGKPLPFGTLGVHKKAAFQDAN
VCLFVFDCIYFNDVSLMDRPLCERRKFLHDNMV
EIPNRIMFSEMKRVTKALDLADMITRVIQEGLEG
LVLKDVKGTYEPGKRHWLKVKKDYLNEGAMADT
ADLVVLGAFYGQGSKGGMMSIFLMGCYDPGSQK
WCTVTKCAGGHDDATLARLQNELDMVKISKDPSK
IPSWLKVNKIYYPDFIVPDPKKAAVWEITGAEF
SKSEAHTADGISIRFPRCTRIRDDKDWKSATNL
PQLKELYQLSKEKADFTVVAGDEGSSTTGGSSEE
NKGPSGSAVSRKAPSKPSASTKKAEGKLSNSNS
KDGNMQTAKPSAMKVGEKLATKSSPVKVGEKRK
AADETLCQTKVLLDIFTGVRLYLPPSTPDFSRLR
RYFVAFDGDLVQEFDMTSATHVLGSRDKNPAAQ
QVSPEWIWACIRKRRLVAPC*VCCLPSPSGHTL
LYHTTGLDSGWRQIDTV*GEWACFSQTHQFSTVS
SGPGISCCGCHS*SQFVLLV*IDLSELGAGFAI
FLFSLKSSLVTLFINKISCSYLCPFPTYTPNNF
PRD*GVKAGLWQLCPQSLLSHPCLFLCTSRLILK
LYLKDCPRKCFCLAELVFSLTRFARNKPHVKEN
KMNFFTFFSVFSYYMVRIPSLKGGD*GQVGKWG
LYEERGGYLGHSSGGIEGSRKICIPKTWKQDCSL
RIKIHMGLEAKNSPCCHQHGDFVPYPILSARVF
QPEVEEATLAAHAISHDCVCSGGGVLLPHDWRN
NYMILLNPQVLADERGHQRPVAFLAYKVNGQYIM
EGFASSFLFTMGGLGFIMLDQSNAPNIPKLNR

Frame 2

LRAASRSRTRI*RDRRSNRRGLPAACNEQVPRP
TVSAGAGEAAICLWLSRSSFHKPSVHSAEKNCA
YSENITGVM*DNSASGQKQICFMDIPSS*EESL
FYHSREAI*DHVPPTLFSCQ
GCMWDSAVAPVRWL
SN
GSVWTMPSVAQLAAKNARKRL*RAYAELAKW
CPIPSQSLGVI*KSGTTLNACLRN*SGPGPPQK
KSRTSQSWKAGKSWKIMRRNR*PSTLQICLLRQQ
VHQRRKLLSRLS*QPLAR*LLQ*KAPHLSPVPI
PGNFLAFQPSPTTLGKPPRAPPLREVCLQANVT
PGIRTVCYGSFESYAPWWPIILATTRRPRSSRTS
FGKAQQEMVSTVMCT*Q*SCCCQESLRLFTT*T
ISRL*SFSVAFLTATQMIWHGT*SRVTCQRQSE
SSLSRASLSPQLPRASLPSRKWMSSFCGCPSSPR
RMSSNRPYRTLPPGVQPMTLNASSG*SNMI*R*
TQVQNMC*TPLTPMPMKPSKPRATCRMWWSGSF
TTRRRWRRSRARDEL*ASRPR**HLCSPCWRRPA
SPLSMQ*RNVPMACSLRSSTMESESRCIRMETT
SATSAAVSSPSFLTRWPTLRTTFPRLFLGATA*
SWILKCF*LTTRQANHCPLGLWEYTRKQPSRMLM
SACLFLIVSTLMMSA*WTDLCVSGGSFFMTTWL
KFQTGSCSQK*SESQKLWTWLT**PG*SRRDWR
GWC*RM*RVHMSLGSGTG*K*RKTI*TRGPWPTQ
LTWWSLEPSMGKGAKAA*CQSSSWAATTLAARS
GAQSPSVQEAMMMPRLPACRMN*TW*RSARTPA
KYPAG*RSTRSTILTSSSQTQRKLPCGRSQGLNS
PNRRLIQLTGSPSDSLAAPESEMIRTGNLPLTF
PNSRNCTSCPRRRQTSL*WLEMRGAPLQGVAVK
RIRVPQGLLCPARPPASPQPVPRKQKGS*VTPTA
KMATCRLQSLPL*RWGRSWPQSLLQ*K*GRSGK
LLMRRCAKQRYCWTSSLGCGFTCHPPHQTSAVS
DATLWHSTGTWYRNLI*LQPRTCWVAGTRTLRPS
RSPQSGFGHVSGNGDW*LPARFAVFPLPQAILS
FTILLDWTQAGGR*TQYRGNGLASPKPTSSPLS
LLDQELVAVGATAEVSLSCWFK*IFQSWVLGLPS
FCFL*KAA*LPFL*IKYLAVIFVLSPPTPPIIS
LEIKE*RLGYGSSVHKAFSPILACSFVLPGSF*
SCI*RTALGNASV*RNLYSA*HALPGTNLM*KKT
K*IFLLSSLCSPITW*GSPV*REGTRVR*ENGA
FMKKGEVIWATPLEG*RAPGRSASPKPGNKTVL
*E*KSTWGLRPRTALVATNMETLYHIPFLVLECS
NLKLKKPPWLHMPSAMTVYALVVVSYFLMTGGI
II*YC*TPKYWLMNVGIRGQ*LSWPTK*MDSIL
WKDLHPASCLQWEV*VS*CWTSRMHQISQNSID

Sequence LIG3.bAug05

GCCTGCGCGCTGCCTCCCGCTCTAGGACCCGGATTTAAAGAGACAGGCGCTCCAACCGTCGTGGGCTGCCCGCGGCCTGTAATGAGCAAGTTCCGAGGCC
TACGGTGAGCGCCGGAGCCGGAGAGGCAGCTATATGTCTTTGGCTTTCAAGATCTTCTTTCCACAAACCCTCCGTGCACTCAGCCGAAAAGAACTGTGCC
TATTCCGAAAACATCACTGGCGTGATGTAAGACAATTCAGCCAGTGGTCAGAAACAGATCTGCTTCATGGACATCCCCTCTTCCTGAGAAGAAAGCCTGT
TCTATCATTCCAGGGAAGCCATCTAAGATCACGTGCCACCTACCTTGTTTTCTTGCCAG
GGTTGCATGTGGGACTCTGCAGTGGCCCCTGTGAGATGGCT
GAGCAAC
GGTTCTGTGTGGACTATGCCAAGCGTGGCACAGCTGGCTGCAAAAAATGCAAGGAAAAGATTGTGAAGGGCGTATGCCGAATTGGCAAAGTGG
TGCCCAATCCCTTCTCAGAGTCTGGGGGTGATATGAAAGAGTGGTACCACATTAAATGCATGTTTGAGAAACTAGAGCGGGCCCGGGCCACCACAAAAAA
AATCGAGGACCTCACAGAGCTGGAAGGCTGGGAAGAGCTGGAAGATAATGAGAAGGAACAGATAACCCAGCACATTGCAGATCTGTCTTCTAAGGCAGCA
GGTACACCAAAGAAGAAAGCTGTTGTCCAGGCTAAGTTGACAACCACTGGCCAGGTGACTTCTCCAGTGAAAGGCGCCTCATTTGTCACCAGTACCAATC
CCCGGAAATTTTCTGGCTTTTCAGCCAAGCCCAACAACTCTGGGGAAGCCCCCTCGAGCCCCACCCCTAAGAGAAGTCTGTCTTCAAGCAAATGTGACCC
CAGGCATAAGGACTGTCTGCTACGGGAGTTTCGAAAGTTATGCGCCATGGTGGCCGATAATCCTAGCTACAACACGAAGACCCAGATCATCCAGGACTTC
CTTCGGAAAGGCTCAGCAGGAGATGGTTTCCACGGTGATGTGTACCTAACAGTGAAGCTGCTGCTGCCAGGAGTCATTAAGACTGTTTACAACTTGAACG
ATAAGCAGATTGTGAAGCTTTTCAGTCGCATTTTTAACTGCAACCCAGATGATATGGCACGGGACCTAGAGCAGGGTGACGTGTCAGAGACAATCAGAGT
CTTCTTTGAGCAGAGCAAGTCTTTCCCCCCAGCTGCCAAGAGCCTCCTTACCATCCAGGAAGTGGATGAGTTCCTTCTGCGGCTGTCCAAGCTCACCAAG
GAGGATGAGCAGCAACAGGCCCTACAGGACATTGCCTCCAGGTGTACAGCCAATGACCTTAAATGCATCATCAGGTTGATCAAACATGATCTGAAGATGA
ACTCAGGTGCAAAACATGTGTTAGACGCCCTTGACCCCAATGCCTATGAAGCCTTCAAAGCCTCGCGCAACCTGCAGGATGTGGTGGAGCGGGTCCTTCA
CAACGCGCAGGAGGTGGAGAAGGAGCCGGGCCAGAGACGAGCTCTGAGCGTCCAGGCCTCGCTGATGACACCTGTGCAGCCCATGTTGGCGGAGGCCTGC
AAGTCCGTTGAGTATGCAATGAAGAAATGTCCCAATGGCATGTTCTCTGAGATCAAGTACGATGGAGAGCGAGTCCAGGTGCATAAGAATGGAGACCACT
TCAGCTACTTCAGCCGCAGTCTCAAGCCCGTCCTTCCTCACAAGGTGGCCCACTTTAAGGACTACATTCCCCAGGCTTTTCCTGGGGGCCACAGCATGAT
CTTGGATTCTGAAGTGCTTCTGATTGACAACAAGACAGGCAAACCACTGCCCTTTGGGACTCTGGGAGTACACAAGAAAGCAGCCTTCCAGGATGCTAAT
GTCTGCCTGTTTGTTTTTGATTGTATCTACTTTAATGATGTCAGCTTGATGGACAGACCTCTGTGTGAGCGGCGGAAGTTTCTTCATGACAACATGGTTG
AAATTCCAAACCGGATCATGTTCTCAGAAATGAAGCGAGTCACAAAAGCTTTGGACTTGGCTGACATGATAACCCGGGTGATCCAGGAGGGATTGGAGGG
GCTGGTGCTGAAGGATGTGAAGGGTACATATGAGCCTGGGAAGCGGCACTGGCTGAAAGTGAAGAAAGACTATTTGAACGAGGGGGCCATGGCCGACACA
GCTGACCTGGTGGTCCTTGGAGCCTTCTATGGGCAAGGGAGCAAAGGCGGCATGATGTCAATCTTCCTCATGGGCTGCTACGACCCTGGCAGCCAGAAGT
GGTGCACAGTCACCAAGTGTGCAGGAGGCCATGATGATGCCACGCTTGCCCGCCTGCAGAATGAACTAGACATGGTGAAGATCAGCAAGGACCCCAGCAA
AATACCCAGCTGGTTGAAGGTCAACAAGATCTACTATCCTGACTTCATCGTCCCAGACCCAAAGAAAGCTGCCGTGTGGGAGATCACAGGGGCTGAATTC
TCCAAATCGGAGGCTCATACAGCTGACGGGATCTCCATCCGATTCCCTCGCTGCACCCGAATCCGAGATGATAAGGACTGGAAATCTGCCACTAACCTTC
CCCAACTCAAGGAACTGTACCAGTTGTCCAAGGAGAAGGCAGACTTCACTGTAGTGGCTGGAGATGAGGGGAGCTCCACTACAGGGGGTAGCAGTGAAGA
GAATAAGGGTCCCTCAGGGTCTGCTGTGTCCCGCAAGGCCCCCAGCAAGCCCTCAGCCAGTACCAAGAAAGCAGAAGGGAAGCTGAGTAACTCCAACAGC
AAAGATGGCAACATGCAGACTGCAAAGCCTTCCGCTATGAAGGTGGGGGAGAAGCTGGCCACAAAGTCTTCTCCAGTGAAAGTAGGGGAGAAGCGGAAAG
CTGCTGATGAGACGCTGTGCCAAACAAAGAGGCGGCCAGCCAGTGAGCAGAGAGGAAGAACTGTGCCAGCAGGCAGGAGATAGAACAGCCCGGCCTAGCC
AGGAGAGACTGCAGGGACTCACTCAGCTGCTGGCCCCAAGTCAAAATTTACATTAAAGGGAAAAGACCAGTCTGGGTGTGGGAATGCAGCATTGAGTTTG
TGGTCAGGGTGGAAGCAGGTCCAGCAAGCAGCGAGTCGGGGAGAGGGCACTGGCTTGGTGACTCTCCTCCCACCTGAGGAGCCTTTTCCCTGTTACATTT
TCTTGTCAGTCTTGGGTTTGGCAACATCTCCTGAGCAATTCTTTTTTTTTTTGAGATAAGTCTCGCTCTGTTGCCTAGGCTGGAGTGAAGTGGTGCAATC
ACAGCGCACTGCAACCTCCGCTCACTGCAACCTCCGCCTCCCAGGTTCAAGCGATTCTCCGGCTTCAGCCTCCCGAGTAGCTGGGAGTATAGGCATGTGC
CACCATGCCCGGCTAATTTTTGTATTTTTAGTAGAGACGGGATTTCACCATGTTGGTCAGGCTGGTCCCAAAGTCCTGACCTCAAGTGATCCGCCTGCCT
TGGTCTCCCAAAGTGCTGGGATTACAGGCCTGAGCCACCATACCCGGCCTCTTTTGAGCACTTTCTGATGCCAAGAACTAGGTTTAGTACTGGCTCAACT
CTGGGGGAGCTGATGCCTCAAAGGACAGATAGAGAAGTAAACATATGATTGACACCCATGTCATTGCGCCCCCACGCTCCCCACCGCCATCCAGGAGTAA
GCATAGAAGTCTCACAGCACAAGGCCTGAACTCGGTCCCCAACAGACCTGTAGAAACCTTTCCCCTCTCTCTTCCCAGCCTGAAGTCCTTGAACCCATTG
AGAGTAGTAAGCAGGACTCCTGACCCCTCAGTCTAGCAGGTTGTACAGAGTAGACTGCTTGGTCTCAGGGGACATCACTGAGTCTGGGGGCACTGAGTCA
GAGCCAGCTCCGCCTGCCCACCATGACTGGGTGGCTCTTATACACATGTACTCTTCCCATCTCCAGGTCCCAGATGTCGAGGCCTGTCCACTCTCCTTTT
CCCCTAGGCAGGGATGGAGGGGCGTGTCAGTCCTGTATAATTTGGAGTGACTGGAGGGGTGGGGGTATTGATGCATGGTATTCCAGTAAACTTCTCTGCT
TGTGTCCTAACTCTAGGCTCCCTCATTCTGTCCCGTGCTCATTTGGGTGAAGACCCATCTGTACCCAGTGTAGGTCTGACCCCACCCTGACCCCTCTGCA
TTTGCAGGTATTGCTGGACATCTTCACTGGGGTGCGGCTTTACTTGCCACCCTCCACACCAGACTTCAGCCGTCTCAGACGCTACTTTGTGGCATTCGAC
GGGGACCTGGTACAGGAATTTGATATGACTTCAGCCACGCACGTGCTGGGTAGCAGGGACAAGAACCCTGCGGCCCAGCAGGTCTCCCCAGAGTGGATTT
GGGCATGTATCCGGAAACGGAGACTGGTAGCTCCCTGCTAGGTTTGCTGTCTTCCCTCTCCCTCAGGCCATACTCTCCTTTACCATACTACTGGACTGGA
CTCAGGCTGGAGGCAGATAGACACAGTATAGGGGGAATGGGCTTGCTTCTCCCAAACCCACCAGTTCTCCACTGTCTCTTCTGGACCAGGAATTAGTTGC
TGTGGGTGCCACAGCTGAAGTCAGTTTGTCTTGCTGGTTTAAATAGATCTTTCAGAGCTGGGTGCTGGGTTTGCCATCTTTTTGTTTTCTTTGAAAAGCA
GCTTAGTTACCCTTTTTATAAATAAAATATCTTGCAGTTATCTTTGTCCTTTCCCCACCTACACCCCCAATAATTTCCCTAGAGATTAAGGAGTAAAGGC
TGGGCTATGGCAGCTCTGTCCACAAAGCCTTCTCTCCCATCCTTGCCTGTTCCTTTGTACTTCCAGGCTCATTTTAAAGTTGTATTTAAAGGACTGCCCT
CGGAAATGCTTCTGTTTAGCGGAACTTGTATTCAGCCTGACACGCTTTGCCAGGAACAAACCTCATGTGAAAGAAAACAAAATGAATTTTTTTACTTTCT
TCTCTGTGTTCTCCTATTACATGGTGAGGATCCCCAGTTTGAAGGGAGGGGACTAGGGTCAGGTAGGAAAATGGGGCCTTTATGAAGAAAGGGGAGGTTA
TTTGGGCCACTCCTCTGGAGGGATAGAGGGCTCCAGGAAGATCTGCATCCCCAAAACCTGGAAACAAGACTGTTCTTTAAGAATAAAAATCCACATGGGG
CTTGAGGCCAAGAACAGCCCTTGTTGCCACCAACATGGAGACTTTGTACCATATCCCATTCTTAGTGCTCGAGTGTTCCAACCTGAAGTTGAAGAAGCCA
CCCTGGCTGCACATGCCATCAGCCATGACTGTGTATGCTCTGGTGGTGGTGTCTTACTTCCTCATGACTGGAGGAATAATTATATGATATTGTTGAACCC
CCAAGTATTGGCTGATGAACGTGGGCATCAGAGGCCAGTAGCTTTCTTGGCCTACAAAGTAAATGGACAGTATATTATGGAAGGATTTGCATCCAGCTTC
CTGTTTACAATGGGAGGTTTAGGTTTCATAATGCTGGACCAGTCGAATGCACCAAATATCCCAAAACTCAATCGATT
  • LIG3.u.f.1 (forward primer)
  • LIG3.u.r.6 (reverse primer)
  • Alternative acceptor

Frame 0

ACALPPALGPGFKETGAPTVVGCPRPVMSKFRG
LR*APEPERQLYVFGFQDLLSTNPPCTQPKRTVP
IPKTSLA*CKTIQPVVRNRSASWTSPLPEKKAC
SIIPGKPSKITCHLPCFLAR
VACGTLQWPL*DG
*AT
VLCGLCQAWHSWLQKMQGKDCEGRMPNWQSG
AQSLLRVWG*YERVVPH*MHV*ETRAGPGHHKK
NRGPHRAGRLGRAGR**EGTDNPAHCRSVF*GS
RYTKEESCCPG*VDNHWPGDFSSERRLICHQYQS
PEIFWLFSQAQQLWGSPLEPHP*EKSVFKQM*P
QA*GLSATGVSKVMRHGGR*S*LQHEDPDHPGL
PSERLSRRWFPR*CVPNSEAAAARSH*DCLQLER
*ADCEAFQSHF*LQPR*YGTGPRAG*RVRDNQS
LL*AEQVFPPSCQEPPYHPGSG*VPSAAVQAHQ
GG*AATGPTGHCLQVYSQ*P*MHHQVDQT*SEDE
LRCKTCVRRP*PQCL*SLQSLAQPAGCGGAGPS
QRAGGGEGAGPETSSERPGLADDTCAAHVGGGL
QVR*VCNEEMSQWHVL*DQVRWRASPGA*EWRPL
QLLQPQSQARPSSQGGPL*GLHSPGFSWGPQHD
LGF*SASD*QQDRQTTALWDSGSTQESSLPGC*
CLPVCF*LYLL**CQLDGQTSV*AAEVSS*QHG*
NSKPDHVLRNEASHKSFGLG*HDNPGDPGGIGG
AGAEGCEGYI*AWEAALAESEERLFERGGHGRH
S*PGGPWSLLWAREQRRHDVNLPHGLLRPWQPEV
VHSHQVCRRP**CHACPPAE*TRHGEDQQGPQQ
NTQLVEGQQDLLS*LHRPRPKESCRVGDHRG*I
LQIGGSYS*RDLHPIPSLHPNPR**GLEICH*PS
PTQGTVPVVQGEGRLHCSGWR*GELHYRG*Q*R
E*GSLRVCCVPQGPQQALSQYQESRREAE*LQQ
QRWQHADCKAFRYEGGGEAGHKVFSSESRGEAES
C**DAVPNKEAASQ*AERKNCASRQEIEQPGLA
RRDCRDSLSCWPQVKIYIKGKRPVWVWECSIEF
VVRVEAGPASSESGRGHWLGDSPPT*GAFSLLHF
LVSLGFGNIS*AILFFFEISLALLPRLE*SGAI
TAHCNLRSLQPPPPRFKRFSGFSLPSSWEYRHV
PPCPANFCIFSRDGISPCWSGWSQSPDLK*SACL
GLPKCWDYRPEPPYPASFEHFLMPRTRFSTGST
LGELMPQRTDREVNI*LTPMSLRPHAPHRHPGV
SIEVSQHKA*TRSPTDL*KPFPSLFPA*SP*TH*
E**AGLLTPQSSRLYRVDCLVSGDITESGGTES
EPAPPAHHDWVALIHMYSSHLQVPDVEACPLSF
SPRQGWRGVSVLYNLE*LEGWGY*CMVFQ*TSLL
VS*L*APSFCPVLIWVKTHLYPV*V*PHPDPSA
FAGIAGHLHWGAALLATLHTRLQPSQTLLCGIR
RGPGTGI*YDFSHARAG*QGQEPCGPAGLPRVDL
GMYPETETGSSLLGLLSSLSLRPYSPLPYYWTG
LRLEADRHSIGGMGLLLPNPPVLHCLFWTRN*L
LWVPQLKSVCLAGLNRSFRAGCWVCHLFVFFEKQ
LSYPFYK*NILQLSLSFPHLHPQ*FP*RLRSKG
WAMAALSTKPSLPSLPVPLYFQAHFKVVFKGLP
SEMLLFSGTCIQPDTLCQEQTSCERKQNEFFYFL
LCVLLLHGEDPQFEGRGLGSGRKMGPL*RKGRL
FGPLLWRDRGLQEDLHPQNLETRLFFKNKNPHG
A*GQEQPLLPPTWRLCTISHS*CSSVPT*S*RSH
PGCTCHQP*LCMLWWWCLTSS*LEE*LYDIVEP
PSIG**TWASEASSFLGLQSKWTVYYGRICIQL
PVYNGRFRFHNAGPVECTKYPKTQSI

Frame 1

PARCLPL*DPDLKRQALQPSWAARGL**ASSEA
YGERRSRRGSYMSLAFKIFFPQTLRALSRKELC
LFRKHHWRDVRQFSQWSETDLLHGHPLFLRRKPV
LSFQGSHLRSRATYLVFLP
GLHVGLCSGPCEMA
EQ
RFCVDYAKRGTAGCKKCKEKIVKGVCRIGKV
VPNPFSESGGDMKEWYHIKCMFEKLERARATTKK
IEDLTELEGWEELEDNEKEQITQHIADLSSKAA
GTPKKKAVVQAKLTTTGQVTSPVKGASFVTSTN
PRKFSGFSAKPNNSGEAPSSPTPKRSLSSSKCDP
RHKDCLLREFRKLCAMVADNPSYNTKTQIIQDF
LRKGSAGDGFHGDVYLTVKLLLPGVIKTVYNLN
DKQIVKLFSRIFNCNPDDMARDLEQGDVSETIRV
FFEQSKSFPPAAKSLLTIQEVDEFLLRLSKLTK
EDEQQQALQDIASRCTANDLKCIIRLIKHDLKM
NSGAKHVLDALDPNAYEAFKASRNLQDVVERVLH
NAQEVEKEPGQRRALSVQASLMTPVQPMLAEAC
KSVEYAMKKCPNGMFSEIKYDGERVQVHKNGDH
FSYFSRSLKPVLPHKVAHFKDYIPQAFPGGHSMI
LDSEVLLIDNKTGKPLPFGTLGVHKKAAFQDAN
VCLFVFDCIYFNDVSLMDRPLCERRKFLHDNMV
EIPNRIMFSEMKRVTKALDLADMITRVIQEGLEG
LVLKDVKGTYEPGKRHWLKVKKDYLNEGAMADT
ADLVVLGAFYGQGSKGGMMSIFLMGCYDPGSQK
WCTVTKCAGGHDDATLARLQNELDMVKISKDPSK
IPSWLKVNKIYYPDFIVPDPKKAAVWEITGAEF
SKSEAHTADGISIRFPRCTRIRDDKDWKSATNL
PQLKELYQLSKEKADFTVVAGDEGSSTTGGSSEE
NKGPSGSAVSRKAPSKPSASTKKAEGKLSNSNS
KDGNMQTAKPSAMKVGEKLATKSSPVKVGEKRK
AADETLCQTKRRPASEQRGRTVPAGRR*NSPA*P
GETAGTHSAAGPKSKFTLKGKDQSGCGNAALSL
WSGWKQVQQAASRGEGTGLVTLLPPEEPFPCYI
FLSVLGLATSPEQFFFFLR*VSLCCLGWSEVVQS
QRTATSAHCNLRLPGSSDSPASASRVAGSIGMC
HHARLIFVFLVETGFHHVGQAGPKVLTSSDPPA
LVSQSAGITGLSHHTRPLLSTF*CQELGLVLAQL
WGS*CLKGQIEK*TYD*HPCHCAPTLPTAIQE*
A*KSHSTRPELGPQQTCRNLSPLSSQPEVLEPI
ESSKQDS*PLSLAGCTE*TAWSQGTSLSLGALSQ
SQLRLPTMTGWLLYTCTLPISRSQMSRPVHSPF
PLGRDGGACQSCIIWSDWRGGGIDAWYSSKLLC
LCPNSRLPHSVPCSFG*RPICTQCRSDPTLTPLH
LQVLLDIFTGVRLYLPPSTPDFSRLRRYFVAFD
GDLVQEFDMTSATHVLGSRDKNPAAQQVSPEWI
WACIRKRRLVAPC*VCCLPSPSGHTLLYHTTGLD
SGWRQIDTV*GEWACFSQTHQFSTVSSGPGISC
CGCHS*SQFVLLV*IDLSELGAGFAIFLFSLKS
SLVTLFINKISCSYLCPFPTYTPNNFPRD*GVKA
GLWQLCPQSLLSHPCLFLCTSRLILKLYLKDCP
RKCFCLAELVFSLTRFARNKPHVKENKMNFFTF
FSVFSYYMVRIPSLKGGD*GQVGKWGLYEERGGY
LGHSSGGIEGSRKICIPKTWKQDCSLRIKIHMG
LEAKNSPCCHQHGDFVPYPILSARVFQPEVEEA
TLAAHAISHDCVCSGGGVLLPHDWRNNYMILLNP
QVLADERGHQRPVAFLAYKVNGQYIMEGFASSF
LFTMGGLGFIMLDQSNAPNIPKLNR

Frame 2

LRAASRSRTRI*RDRRSNRRGLPAACNEQVPRP
TVSAGAGEAAICLWLSRSSFHKPSVHSAEKNCA
YSENITGVM*DNSASGQKQICFMDIPSS*EESL
FYHSREAI*DHVPPTLFSCQ
GCMWDSAVAPVRWL
SN
GSVWTMPSVAQLAAKNARKRL*RAYAELAKW
CPIPSQSLGVI*KSGTTLNACLRN*SGPGPPQK
KSRTSQSWKAGKSWKIMRRNR*PSTLQICLLRQQ
VHQRRKLLSRLS*QPLAR*LLQ*KAPHLSPVPI
PGNFLAFQPSPTTLGKPPRAPPLREVCLQANVT
PGIRTVCYGSFESYAPWWPIILATTRRPRSSRTS
FGKAQQEMVSTVMCT*Q*SCCCQESLRLFTT*T
ISRL*SFSVAFLTATQMIWHGT*SRVTCQRQSE
SSLSRASLSPQLPRASLPSRKWMSSFCGCPSSPR
RMSSNRPYRTLPPGVQPMTLNASSG*SNMI*R*
TQVQNMC*TPLTPMPMKPSKPRATCRMWWSGSF
TTRRRWRRSRARDEL*ASRPR**HLCSPCWRRPA
SPLSMQ*RNVPMACSLRSSTMESESRCIRMETT
SATSAAVSSPSFLTRWPTLRTTFPRLFLGATA*
SWILKCF*LTTRQANHCPLGLWEYTRKQPSRMLM
SACLFLIVSTLMMSA*WTDLCVSGGSFFMTTWL
KFQTGSCSQK*SESQKLWTWLT**PG*SRRDWR
GWC*RM*RVHMSLGSGTG*K*RKTI*TRGPWPTQ
LTWWSLEPSMGKGAKAA*CQSSSWAATTLAARS
GAQSPSVQEAMMMPRLPACRMN*TW*RSARTPA
KYPAG*RSTRSTILTSSSQTQRKLPCGRSQGLNS
PNRRLIQLTGSPSDSLAAPESEMIRTGNLPLTF
PNSRNCTSCPRRRQTSL*WLEMRGAPLQGVAVK
RIRVPQGLLCPARPPASPQPVPRKQKGS*VTPTA
KMATCRLQSLPL*RWGRSWPQSLLQ*K*GRSGK
LLMRRCAKQRGGQPVSREEELCQQAGDRTARPS
QERLQGLTQLLAPSQNLH*REKTSLGVGMQH*VC
GQGGSRSSKQRVGERALAW*LSSHLRSLFPVTF
SCQSWVWQHLLSNSFFF*DKSRSVA*AGVKWCN
HSALQPPLTATSASQVQAILRLQPPE*LGV*ACA
TMPG*FLYF**RRDFTMLVRLVPKS*PQVIRLP
WSPKVLGLQA*ATIPGLF*ALSDAKN*V*YWLN
SGGADASKDR*RSKHMIDTHVIAPPRSPPPSRSK
HRSLTAQGLNSVPNRPVETFPLSLPSLKSLNPL
RVVSRTPDPSV*QVVQSRLLGLRGHH*VWGH*V
RASSACPP*LGGSYTHVLFPSPGPRCRGLSTLLF
P*AGMEGRVSPV*FGVTGGVGVLMHGIPVNFSA
CVLTLGSLILSRAHLGEDPSVPSVGLTPP*PLC
ICRYCWTSSLGCGFTCHPPHQTSAVSDATLWHST
GTWYRNLI*LQPRTCWVAGTRTLRPSRSPQSGF
GHVSGNGDW*LPARFAVFPLPQAILSFTILLDW
TQAGGR*TQYRGNGLASPKPTSSPLSLLDQELVA
VGATAEVSLSCWFK*IFQSWVLGLPSFCFL*KA
A*LPFL*IKYLAVIFVLSPPTPPIISLEIKE*R
LGYGSSVHKAFSPILACSFVLPGSF*SCI*RTAL
GNASV*RNLYSA*HALPGTNLM*KKTK*IFLLS
SLCSPITW*GSPV*REGTRVR*ENGAFMKKGEV
IWATPLEG*RAPGRSASPKPGNKTVL*E*KSTWG
LRPRTALVATNMETLYHIPFLVLECSNLKLKKP
PWLHMPSAMTVYALVVVSYFLMTGGIII*YC*T
PKYWLMNVGIRGQ*LSWPTK*MDSILWKDLHPAS
CLQWEV*VS*CWTSRMHQISQNSID

Sequence LIG3.dAug05

GCCTGCGCGCTGCCTCCCGCTCTAGGACCCGGATTTAAAGAGACAGGCGCTCCAACCGTCGTGGGCTGCCCGCGGCCTGTAATGAGCAAGTTCCGAGGCC
TACGGTGAGCGCCGGAGCCGGAGAGGCAGCTATATGTCTTTGGCTTTCAAGATCTTCTTTCCACAAACCCTCCGTGCACTCAGCCGAAAAGAACTGTGCC
TATTCCGAAAACATCACTGGCGTGATGTAAGACAATTCAGCCAGTGGTCAGAAACAGATCTGCTTCATGGACATCCCCTCTTCCTGAGAAGAAAGCCTGT
TCTATCATTCCAGGGAAGCCATCTAAGATCACGTGCCACCTACCTTGTTTTCTTGCCAG
GGTTGCATGTGGGACTCTGCAGTGGCCCCTGTGAGATGGCT
GAGCAAC
GGTTCTGTGTGGACTATGCCAAGCGTGGCACAGCTGGCTGCAAAAAATGCAAGGAAAAGATTGTGAAGGGCGTATGCCGAATTGGCAAAGTGG
TGCCCAATCCCTTCTCAGAGTCTGGGGGTGATATGAAAGAGTGGTACCACATTAAATGCATGTTTGAGAAACTAGAGCGGGCCCGGGCCACCACAAAAAA
AATCGAGGACCTCACAGAGCTGGAAGGCTGGGAAGAGCTGGAAGATAATGAGAAGGAACAGATAACCCAGCACATTGCAGATCTGTCTTCTAAGGCAGCA
GGTACACCAAAGAAGAAAGCTGTTGTCCAGGCTAAGTTGACAACCACTGGCCAGGTGACTTCTCCAGTGAAAGGCGCCTCATTTGTCACCAGTACCAATC
CCCGGAAATTTTCTGGCTTTTCAGCCAAGCCCAACAACTCTGGGGAAGCCCCCTCGAGCCCCACCCCTAAGAGAAGTCTGTCTTCAAGCAAATGTGACCC
CAGGCATAAGGACTGTCTGCTACGGGAGTTTCGAAAGTTATGCGCCATGGTGGCCGATAATCCTAGCTACAACACGAAGACCCAGATCATCCAGGACTTC
CTTCGGAAAGGCTCAGCAGGAGGTGTGGCATGAGCATCCTGAATAGGCCTTTCCTCCGGAGAGCTCAAGGCCATGGCTGCCTTCCTGGGCATGTGAGCTG
TATAGTTGCCCGGGATCCCATGCTTGGTTTAATGCTGTACTGTAGTGCTATCTGGAAATTCTTAATAATTTTGAACAAGGGGCCTCGCATTTGCATTTTG
CACTGTACTCCACAAATTATGTAGCCAGTTTTTCTCAGAGCACTTTGGCATCTGTCTTCCTCTCCCCTTATTCTGCTCCTAGGAGGCCAGAAGAACCCAT
CCTATTTGACTAGTTAGAAAATTTACTGTCCTACATATTTAATTAGGTTTTTGTGAACCATATACACAGTTTGGAATAGAATCTTCCAACTCTG
  • LIG3.u.f.1 (forward primer)
  • LIG3.u.r.6 (reverse primer)
  • Alternative acceptor

Frame 0

ACALPPALGPGFKETGAPTVVGCPRPVMSKFRG
LR*APEPERQLYVFGFQDLLSTNPPCTQPKRTVP
IPKTSLA*CKTIQPVVRNRSASWTSPLPEKKAC
SIIPGKPSKITCHLPCFLAR
VACGTLQWPL*DG
*AT
VLCGLCQAWHSWLQKMQGKDCEGRMPNWQSG
AQSLLRVWG*YERVVPH*MHV*ETRAGPGHHKK
NRGPHRAGRLGRAGR**EGTDNPAHCRSVF*GS
RYTKEESCCPG*VDNHWPGDFSSERRLICHQYQS
PEIFWLFSQAQQLWGSPLEPHP*EKSVFKQM*P
QA*GLSATGVSKVMRHGGR*S*LQHEDPDHPGL
PSERLSRRCGMSILNRPFLRRAQGHGCLPGHVSC
IVARDPMLGLMLYCSAIWKFLIILNKGPRICIL
HCTPQIM*PVFLRALWHLSSSPLILLLGGQKNP
SYLTS*KIYCPTYLIRFL*TIYTVWNRIFQL

Frame 1

PARCLPL*DPDLKRQALQPSWAARGL**ASSEA
YGERRSRRGSYMSLAFKIFFPQTLRALSRKELC
LFRKHHWRDVRQFSQWSETDLLHGHPLFLRRKPV
LSFQGSHLRSRATYLVFLP
GLHVGLCSGPCEMA
EQ
RFCVDYAKRGTAGCKKCKEKIVKGVCRIGKV
VPNPFSESGGDMKEWYHIKCMFEKLERARATTKK
IEDLTELEGWEELEDNEKEQITQHIADLSSKAA
GTPKKKAVVQAKLTTTGQVTSPVKGASFVTSTN
PRKFSGFSAKPNNSGEAPSSPTPKRSLSSSKCDP
RHKDCLLREFRKLCAMVADNPSYNTKTQIIQDF
LRKGSAGGVA*AS*IGLSSGELKAMAAFLGM*A
V*LPGIPCLV*CCTVVLSGNS**F*TRGLAFAFC
TVLHKLCSQFFSEHFGICLPLPLFCS*EARRTH
PI*LVRKFTVLHI*LGFCEPYTQFGIESSNS

Frame 2

LRAASRSRTRI*RDRRSNRRGLPAACNEQVPRP
TVSAGAGEAAICLWLSRSSFHKPSVHSAEKNCA
YSENITGVM*DNSASGQKQICFMDIPSS*EESL
FYHSREAI*DHVPPTLFSCQ
GCMWDSAVAPVRWL
SN
GSVWTMPSVAQLAAKNARKRL*RAYAELAKW
CPIPSQSLGVI*KSGTTLNACLRN*SGPGPPQK
KSRTSQSWKAGKSWKIMRRNR*PSTLQICLLRQQ
VHQRRKLLSRLS*QPLAR*LLQ*KAPHLSPVPI
PGNFLAFQPSPTTLGKPPRAPPLREVCLQANVT
PGIRTVCYGSFESYAPWWPIILATTRRPRSSRTS
FGKAQQEVWHEHPE*AFPPESSRPWLPSWACEL
YSCPGSHAWFNAVL*CYLEILNNFEQGASHLHF
ALYSTNYVASFSQSTLASVFLSPYSAPRRPEEPI
LFD*LENLLSYIFN*VFVNHIHSLE*NLPTL

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