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Sequences

Sequence STAT1.bAug05

GGTGGGGGCGGAAGGGGGCCGGGCGCCAGCGCTGCCTTTTCTCCTGCCGGGTAGTTTCGCTTTCCTGCGCAGAGTCTGCGGAGGGGCTCGGCTGCACCGG GGGGATCGCGCCTGGCAGACCCCAGACCGAGCAGAGGCGACCCAGCGCGCTCGGGAGAGGCTGCACCGCCGCGCCCCCGCCTAGCCCTTCCGGATCCTGC GCGCAGAGATGTCTCAGTGGTACGAACTTCAGCAGCTTGACTCAAAATTCCTGGAGCAGGTTCACCAGCTTTATGATGACAGTTTTCCCATGGAAATCAG ACAGTACCTGGCACAGTGGTTAGAAAAGCAAGACTGGGAGCACGCTGCCAATGATGTTTCATTTGCCACCATCCGTTTTCATGACCTCCTGTCACAGCTG GATGATCAATATAGTCGCTTTTCTTTGGAGAATAACTTCTTGCTACAGCATAACATAAGGAAAAGCAAGCGTAATCTTCAGGATAATTTTCAGGAAGACC CAATCCAGATGTCTATGATCATTTACAGCTGTCTGAAGGAAGAAAGGAAAATTCTGGAAAACGCCCAGAGATTTAATCAGGCTCAGTCGGGGAATATTCA GAGCACAGTGATGTTAGACAAACAGAAAGAGCTTGACAGTAAAGTCAGAAATGTGAAGGACAAGGTTATGTGTATAGAGCATGAAATCAAGAGCCTGGAA GATTTACAAGATGAATATGACTTCAAATGCAAAACCTTGCAGAACAGAGAACACGAGACCAATGGTGTGGCAAAGAGTGATCAGAAACAAGAACAGCTGT TACTCAAGAAGATGTATTTAATGCTTGACAATAAGAGAAAGGAAGTAGTTCACAAAATAATAGAGTTGCTGAATGTCACTGAACTTACCCAGAATGCCCT GATTAATGATGAACTAGTGGAGTGGAAGCGGAGACAGCAGAGCGCCTGTATTGGGGGGCCGCCCAATGCTTGCTTGGATCAGCTGCAGAACTGGTTCACT ATAGTTGCGGAGAGTCTGCAGCAAGTTCGGCAGCAGCTTAAAAAGTTGGAGGAATTGGAACAGAAATACACCTACGAACATGACCCTATCACAAAAAACA AACAAGTGTTATGGGACCGCACCTTCAGTCTTTTCCAGCAGCTCATTCAGAGCTCGTTTGTGGTGGAAAGACAGCCCTGCATGCCAACGCACCCTCAGAG GCCGCTGGTCTTGAAGACAGGGGTCCAGTTCACTGTGAAGTTGAGACTGTTGGTGAAATTGCAAGAGCTGAATTATAATTTGAAAGTCAAAGTCTTATTT GATAAAGATGTGAATGAGAGAAATACAGTAAAAGGATTTAGGAAGTTCAACATTTTGGGCACGCACACAAAAGTGATGAACATGGAGGAGTCCACCAATG GCAGTCTGGCGGCTGAATTTCGGCACCTGCAATTGAAAGAACAGAAAAATGCTGGCACCAGAACGAATGAGGGTCCTCTCATCGTTACTGAAGAGCTTCA CTCCCTTAGTTTTGAAACCCAATTGTGCCAGCCTGGTTTGGTAATTGACCTCGAGACGACCTCTCTGCCCGTTGTGGTGATCTCCAACGTCAGCCAGCTC CCGAGCGGTTGGGCCTCCATCCTTTGGTACAACATGCTGGTGGCGGAACCCAGGAATCTGTCCTTCTTCCTGACTCCACCATGTGCACGATGGGCTCAGC TTTCAGAAGTGCTGAGTTGGCAGTTTTCTTCTGTCACCAAAAGAGGTCTCAATGTGGACCAGCTGAACATGTTGGGAGAGAAGCTTCTTGGTCCTAACGC CAGCCCCGATGGTCTCATTCCGTGGACGAGGTTTTGTAAGGAAAATATAAATGATAAAAATTTTCCCTTCTGGCTTTGGATTGAAAGCATCCTAGAACTC ATTAAAAAACACCTGCTCCCTCTCTGGAATGATGGGTGCATCATGGGCTTCATCAGCAAGGAGCGAGAGCGTGCCCTGTTGAAGGACCAGCAGCCGGGGA CCTTCCTGCTGCGGTTCAGTGAGAGCTCCCGGGAAGGGGCCATCACATTCACATGGGTGGAGCGGTCCCAGAACGGAGGCGAACCTGACTTCCATGCGGT TGAACCCTACACGAAGAAAGAACTTTCTGCTGTTACTTTCCCTGACATCATTCGCAATTACAAAGTCATGGCTGCTGAGAATATTCCTGAGAATCCCCTG AAGTATCTGTATCCAAATATTGACAAAGACCATGCCTTTGGAAAGTATTACTCCAGGCCAAAGGAAGCACCAGAGCCAATGGAACTTGATGGCCCTAAAG GAACTGGATATATCAAGACTGAGTTGATTTCTGTGTCTGAAGTTCACCCTTCTAGACTTCAGACCACAGACAACCTGCTCCCCATGTCTCCTGAGGAGTT TGACGAGGTGTCTCGGATAGTGGGCTCTGTAGAATTCGACAGTATGATGAACACAGTATAGAGCATGAATTTTTTTCATCTTCTCTGGCGACAGTTTTCC TTCTCATCTGTGATTCCCTCCTGCTACTCTGTTCCTTCACATCCTGTGTTTCTAGGGAAATGAAAGAAAGGCCAGCAAATTCGCTGCAACCTGTTGATAG CAAGTGAATTTTTCTCTAACTCAGAAACATCAGTTACTCTGAAGGGCATCATGCATCTTACTGAAGGTAAAATTGAAAGGCATTCTCTGAAGAGTGGGTT TCACAAGTGAAAAACATCCAGATACACCCAAAGTATCAGGACGAGAATGAGGGTCCTTTGGGAAAGGAGAAGTTAAGCAACATCTAGCAAATGTTATGCA TAAAGTCAGTGCCCAACTGTTATAGGTTGTTGGATAAATCAGTGGTTATTTAGGGAACTGCTTGACGTAGGAACGGTAAATTTCTGTGGGAGAATTCTTA CATGTTTTCTTTGCTTTAAGTGTAACTGGCAGTTTTCCATTGGTTTACCTGTGAAATAGTTCAAAGCCAAGTTTATATACAATTATATCAGTCCTCTTTC AAAGGTAGCCATCATGGATCTGGTAGGGGGAAAATGTGTATTTTATTACATCTTTCACATTGGCTATTTAAAGACAAAGACAAATTCTAAGGATGTACAT TTCCAAATTCACAAGTTGTGTTTGATATCCAAAGCTGAATACATTCTGCTTTCATCTTGGTCACATACAATTATTTTTACAGTTCTCCCAAGGGAGTTAG GCTATTCACAACCACTCATTCAAAAGTTGAAATTAACCATAGATGTAGATAAACTCAGAAATTTAATTCATGTTTCTTAAATGGGCTACTTTGTCCTTTT TGTTATTAGGGTGGTATTTAGTCTATTAGCCACAAAATTGGGAAAGGAGTAGAAAAAGCAGTAACTGACAACTTGAATAATACACCAGAGATAATATGAG AATCAGATCATTTCAAAACTCATTTCCTATGTAACTGCATTGAGAACTGCATATGTTTCGCTGATATATGTGTTTTTCACATTTGCGAATGGTTCCATTC TCTCTCCTGTACTTTTTCCAGACACTTTTTTGAGTGGATGATGTTTCGTGAAGTATACTGTATTTTTACCTTTTTCCTTCCTTATCACTGACACAAAAAG TAGATTAAGAGATGGGTTTGACAAGGTTCTTCCCTTTTACATACTGCTGTCTATGTGGCTGTATCTTGTTTTTCCACTACTGCTACCACAACTATATTAT CATGCAAATGCTGTATTCTTCTTTGGTGGAGATAAAGATTTCTTGAGTTTTGTTTTAAAATTAAAGCTAAAGTATCTGTATTGCATTAAATATAATATGC ACACAGTGCTTTCCGTGGCACTGCATACAATCTGAGGCCTCCTCTCTCAGTTTTTATATAGATGGCGAGAACCTAAGTTTCAGTTGATTTTACAATTGAA ATGACTAAAAAACAAAGAAGACAACATTAAAACAATATTGTTTCTAATTGCTGAGGTTTAGCTGTCAGTTCTTTTTGCCCTTTGGGAATTCGGCATGGTT TCATTTTACTGCACTAGCCAAGAGACTTTACTTTTAAGAAGTATTAAAATTCTAAAATTCTATTAA

STAT1.u.f.1 (forward primer)
STAT1.u.r.5 (reverse primer)
Multiple exon skipping

Frame 0

GGGGRGPGASAAFSPAG*FRFPAQSLRRGSAAP GGSRLADPRPSRGDPARSGEAAPPRPRLALPDPA RRDVSVVRTSAA*LKIPGAGSPAL**QFSHGNQ TVPGTVVRKARLGARCQ*CFICHHPFS*PPVTA G*SI*SLFFGE*LLATA*HKEKQA*SSG*FSGRP NPDVYDHLQLSEGRKENSGKRPEI*SGSVGEYS EHSDVRQTERA*Q*SQKCEGQGYVYRA*NQEPG RFTR*I*LQMQNLAEQRTRDQWCGKE*SETRTAV TQEDVFNA*Q*EKGSSSQNNRVAECH*TYPECP D***TSGVEAETAERLYWGAAQCLLGSAAELVH YSCGESAASSAAA*KVGGIGTEIHLRT*PYHKKQ TSVMGPHLQSFPAAHSELVCGGKTALHANAPSE AAGLEDRGPVHCEVETVGEIARAEL*FESQSLI **RCE*EKYSKRI*EVQHFGHAHKSDEHGGVHQW QSGG*ISAPAIERTEKCWHQNE*GSSHRY*RAS LP*F*NPIVPAWFGN*PRDDLSARCGDLQRQPA PERLGLHPLVQHAGGGTQESVLLPDSTMCTMGSA FRSAELAVFFCHQKRSQCGPAEHVGREASWS*R QPRWSHSVDEVL*GKYK**KFSLLALD*KHPRT H*KTPAPSLE*WVHHGLHQQGARACPVEGPAAGD LPAAVQ*ELPGRGHHIHMGGAVPERRRT*LPCG *TLHEERTFCCYFP*HHSQLQSHGC*EYS*ESP EVSVSKY*QRPCLWKVLLQAKGSTRANGT*WP*R NWIYQD*VDFCV*SSPF*TSDHRQPAPHVS*GV *RGVSDSGLCRIRQYDEHSIEHEFFSSSLATVF LLICDSLLLLCSFTSCVSREMKERPANSLQPVDS K*IFL*LRNISYSEGHHASY*R*N*KAFSEEWV SQVKNIQIHPKYQDENEGPLGKEKLSNI*QMLC IKSVPNCYRLLDKSVVI*GTA*RRNGKFLWENSY MFSLL*V*LAVFHWFTCEIVQSQVYIQLYQSSF KGSHHGSGRGKMCILLHLSHWLFKDKDKF*GCT FPNSQVVFDIQS*IHSAFILVTYNYFYSSPKGVR LFTTTHSKVEINHRCR*TQKFNSCFLNGLLCPF CY*GGI*SISHKIGKGVEKAVTDNLNNTPEII* ESDHFKTHFLCNCIENCICFADICVFHICEWFHS LSCTFSRHFFEWMMFREVYCIFTFFLPYH*HKK *IKRWV*QGSSLLHTAVYVAVSCFSTTATTTIL SCKCCILLWWR*RFLEFCFKIKAKVSVLH*I*YA HSAFRGTAYNLRPPLSVFI*MART*VSVDFTIE MTKKQRRQH*NNIVSNC*GLAVSSFCPLGIRHG FILLH*PRDFTFKKY*NSKILL

Frame 1

VGAEGGRAPALPFLLPGSFAFLRRVCGGARLHR GDRAWQTPDRAEATQRARERLHRRAPA*PFRIL RAEMSQWYELQQLDSKFLEQVHQLYDDSFPMEIR QYLAQWLEKQDWEHAANDVSFATIRFHDLLSQL DDQYSRFSLENNFLLQHNIRKSKRNLQDNFQED PIQMSMIIYSCLKEERKILENAQRFNQAQSGNIQ STVMLDKQKELDSKVRNVKDKVMCIEHEIKSLE DLQDEYDFKCKTLQNREHETNGVAKSDQKQEQL LLKKMYLMLDNKRKEVVHKIIELLNVTELTQNAL INDELVEWKRRQQSACIGGPPNACLDQLQNWFT IVAESLQQVRQQLKKLEELEQKYTYEHDPITKN KQVLWDRTFSLFQQLIQSSFVVERQPCMPTHPQR PLVLKTGVQFTVKLRLLVKLQELNYNLKVKVLF DKDVNERNTVKGFRKFNILGTHTKVMNMEESTN GSLAAEFRHLQLKEQKNAGTRTNEGPLIVTEELH SLSFETQLCQPGLVIDLETTSLPVVVISNVSQL PSGWASILWYNMLVAEPRNLSFFLTPPCARWAQ LSEVLSWQFSSVTKRGLNVDQLNMLGEKLLGPNA SPDGLIPWTRFCKENINDKNFPFWLWIESILEL IKKHLLPLWNDGCIMGFISKERERALLKDQQPG TFLLRFSESSREGAITFTWVERSQNGGEPDFHAV EPYTKKELSAVTFPDIIRNYKVMAAENIPENPL KYLYPNIDKDHAFGKYYSRPKEAPEPMELDGPK GTGYIKTELISVSEVHPSRLQTTDNLLPMSPEEF DEVSRIVGSVEFDSMMNTV*SMNFFHLLWRQFS FSSVIPSCYSVPSHPVFLGK*KKGQQIRCNLLI ASEFFSNSETSVTLKGIMHLTEGKIERHSLKSGF HK*KTSRYTQSIRTRMRVLWERRS*ATSSKCYA *SQCPTVIGCWINQWLFRELLDVGTVNFCGRIL TCFLCFKCNWQFSIGLPVK*FKAKFIYNYISPLS KVAIMDLVGGKCVFYYIFHIGYLKTKTNSKDVH FQIHKLCLISKAEYILLSSWSHTIIFTVLPREL GYSQPLIQKLKLTIDVDKLRNLIHVS*MGYFVLF VIRVVFSLLATKLGKE*KKQ*LTT*IIHQR*YE NQIISKLISYVTALRTAYVSLIYVFFTFANGSI LSPVLFPDTFLSG*CFVKYTVFLPFSFLITDTKS RLRDGFDKVLPFYILLSMWLYLVFPLLLPQLYY HANAVFFFGGDKDFLSFVLKLKLKYLYCIKYNM HTVLSVALHTI*GLLSQFLYRWREPKFQLILQLK *LKNKEDNIKTILFLIAEV*LSVLFALWEFGMV SFYCTSQETLLLRSIKILKFY*

Frame 2

WGRKGAGRQRCLFSCRVVSLSCAESAEGLGCTG GIAPGRPQTEQRRPSALGRGCTAAPPPSPSGSC AQRCLSGTNFSSLTQNSWSRFTSFMMTVFPWKS DSTWHSG*KSKTGSTLPMMFHLPPSVFMTSCHSW MINIVAFLWRITSCYSIT*GKASVIFRIIFRKT QSRCL*SFTAV*RKKGKFWKTPRDLIRLSRGIF RAQ*C*TNRKSLTVKSEM*RTRLCV*SMKSRAWK IYKMNMTSNAKPCRTENTRPMVWQRVIRNKNSC YSRRCI*CLTIRERK*FTK**SC*MSLNLPRMP *LMMN*WSGSGDSRAPVLGGRPMLAWISCRTGSL *LRRVCSKFGSSLKSWRNWNRNTPTNMTLSQKT NKCYGTAPSVFSSSSFRARLWWKDSPACQRTLR GRWS*RQGSSSL*S*DCW*NCKS*III*KSKSYL IKM*MREIQ*KDLGSSTFWARTQK**TWRSPPM AVWRLNFGTCN*KNRKMLAPERMRVLSSLLKSF TPLVLKPNCASLVW*LTSRRPLCPLW*SPTSASS RAVGPPSFGTTCWWRNPGICPSS*LHHVHDGLS FQKC*VGSFLLSPKEVSMWTS*TCWERSFLVLT PAPMVSFRGRGFVRKI*MIKIFPSGFGLKAS*NS LKNTCSLSGMMGASWASSARSESVPC*RTSSRG PSCCGSVRAPGKGPSHSHGWSGPRTEANLTSMR LNPTRRKNFLLLLSLTSFAITKSWLLRIFLRIP* SICIQILTKTMPLESITPGQRKHQSQWNLMALK ELDISRLS*FLCLKFTLLDFRPQTTCSPCLLRS LTRCLG*WAL*NSTV**TQYRA*IFFIFSGDSFP SHL*FPPATLFLHILCF*GNERKASKFAATC** QVNFSLTQKHQLL*RASCILLKVKLKGIL*RVG FTSEKHPDTPKVSGRE*GSFGKGEVKQHLANVMH KVSAQLL*VVG*ISGYLGNCLT*ER*ISVGEFL HVFFALSVTGSFPLVYL*NSSKPSLYTIISVLF QR*PSWIW*GENVYFITSFTLAI*RQRQILRMYI SKFTSCV*YPKLNTFCFHLGHIQLFLQFSQGS* AIHNHSFKS*N*P*M*INSEI*FMFLKWATLSF LLLGWYLVY*PQNWERSRKSSN*QLE*YTRDNMR IRSFQNSFPM*LH*ELHMFR*YMCFSHLRMVPF SLLYFFQTLF*VDDVS*SILYFYLFPSLSLTQK VD*EMGLTRFFPFTYCCLCGCILFFHYCYHNYII MQMLYSSLVEIKIS*VLF*N*S*SICIALNIIC TQCFPWHCIQSEASSLSFYIDGENLSFS*FYN* ND*KTKKTTLKQYCF*LLRFSCQFFLPFGNSAWF HFTALAKRLYF*EVLKF*NSI

Sequence STAT1.dAug05

GGTGGGGGCGGAAGGGGGCCGGGCGCCAGCGCTGCCTTTTCTCCTGCCGGGTAGTTTCGCTTTCCTGCGCAGAGTCTGCGGAGGGGCTCGGCTGCACCGG GGGGATCGCGCCTGGCAGACCCCAGACCGAGCAGAGGCGACCCAGCGCGCTCGGGAGAGGCTGCACCGCCGCGCCCCCGCCTAGCCCTTCCGGATCCTGC GCGCAGAAAAGTTTCATTTGCTGTATGCCATCCTCGAGAGCTGTCTAGGTTAACGTTCGCACTCTGTGTATATAACCTCGACAGTCTTGGCACCTAACGT GCTGTGCGTAGCTGCTCCTTTGGTTGAATCCCCAGGCCCTTGTTGGGGCACAAGGTGGCAGGGATACACCAGTGCACAGAATCCTCCACCAACATCCATC CCTTCAAGTGGTGAAAACAGACAATAATGAAAATAAAATATGTGGATGTCTCAGTGGTACGAACTTCAGCAGCTTGACTCAAAATTCCTGGAGCAGGTTC ACCAGCTTTATGATGACAGTTTTCCCATGGAAATCAGACAGTACCTGGCACAGTGGTTAGAAAAGCAAGACTGGGAGCACGCTGCCAATGATGTTTCATT TGCCACCATCCGTTTTCATGACCTCCTGTCACAGCTGGATGATCAATATAGTCGCTTTTCTTTGGAGAATAACTTCTTGCTACAGCATAACATAAGGAAA AGCAAGCGTAATCTTCAGGATAATTTTCAGGAAGACCCAATCCAGATGTCTATGATCATTTACAGCTGTCTGAAGGAAGAAAGGAAAATTCTGGAAAACG CCCAGAGATTTAATCAGGCTCAGTCGGGGAATATTCAGAGCACAGTGATGTTAGACAAACAGAAAGAGCTTGACAGTAAAGTCAGAAATGTGAAGGACAA GGTTATGTGTATAGAGCATGAAATCAAGAGCCTGGAAGATTTACAAGATGAATATGACTTCAAATGCAAAACCTTGCAGAACAGAGAACACGAGACCAAT GGTGTGGCAAAGAGTGATCAGAAACAAGAACAGCTGTTACTCAAGAAGATGTATTTAATGCTTGACAATAAGAGAAAGGAAGTAGTTCACAAAATAATAG AGTTGCTGAATGTCACTGAACTTACCCAGAATGCCCTGATTAATGATGAACTAGTGGAGTGGAAGCGGAGACAGCAGAGCGCCTGTATTGGGGGGCCGCC CAATGCTTGCTTGGATCAGCTGCAGAACTGGTTCACTATAGTTGCGGAGAGTCTGCAGCAAGTTCGGCAGCAGCTTAAAAAGTTGGAGGAATTGGAACAG AAATACACCTACGAACATGACCCTATCACAAAAAACAAACAAGTGTTATGGGACCGCACCTTCAGTCTTTTCCAGCAGCTCATTCAGAGCTCGTTTGTGG TGGAAAGACAGCCCTGCATGCCAACGCACCCTCAGAGGCCGCTGGTCTTGAAGACAGGGGTCCAGTTCACTGTGAAGTTGAGACTGTTGGTGAAATTGCA AGAGCTGAATTATAATTTGAAAGTCAAAGTCTTATTTGATAAAGATGTGAATGAGAGAAATACAGTAAAAGGATTTAGGAAGTTCAACATTTTGGGCACG CACACAAAAGTGATGAACATGGAGGAGTCCACCAATGGCAGTCTGGCGGCTGAATTTCGGCACCTGCAATTGAAAGAACAGAAAAATGCTGGCACCAGAA CGAATGAGGGTCCTCTCATCGTTACTGAAGAGCTTCACTCCCTTAGTTTTGAAACCCAATTGTGCCAGCCTGGTTTGGTAATTGACCTCGAGACGACCTC TCTGCCCGTTGTGGTGATCTCCAACGTCAGCCAGCTCCCGAGCGGTTGGGCCTCCATCCTTTGGTACAACATGCTGGTGGCGGAACCCAGGAATCTGTCC TTCTTCCTGACTCCACCATGTGCACGATGGGCTCAGCTTTCAGAAGTGCTGAGTTGGCAGTTTTCTTCTGTCACCAAAAGAGGTCTCAATGTGGACCAGC TGAACATGTTGGGAGAGAAGCTTCTTGGTCCTAACGCCAGCCCCGATGGTCTCATTCCGTGGACGAGGTTTTGTAAGGAAAATATAAATGATAAAAATTT TCCCTTCTGGCTTTGGATTGAAAGCATCCTAGAACTCATTAAAAAACACCTGCTCCCTCTCTGGAATGATGGGTGCATCATGGGCTTCATCAGCAAGGAG CGAGAGCGTGCCCTGTTGAAGGACCAGCAGCCGGGGACCTTCCTGCTGCGGTTCAGTGAGAGCTCCCGGGAAGGGGCCATCACATTCACATGGGTGGAGC GGTCCCAGAACGGAGGCGAACCTGACTTCCATGCGGTTGAACCCTACACGAAGAAAGAACTTTCTGCTGTTACTTTCCCTGACATCATTCGCAATTACAA AGTCATGGCTGCTGAGAATATTCCTGAGAATCCCCTGAAGTATCTGTATCCAAATATTGACAAAGACCATGCCTTTGGAAAGTATTACTCCAGGCCAAAG GAAGCACCAGAGCCAATGGAACTTGATGGCCCTAAAGGAACTGGATATATCAAGACTGAGTTGATTTCTGTGTCTGAAGTGTAAGTGAACACAGAAGAGT GACATGTTTACAAACCTCAAGCCAGCCTTGCTCCTGGCTGGGGCCTGTTGAAGATGCTTGTATTTTACTTTTCCATTGTAATTGCTATCGCCATCACAGC TGAACTTGTTGAGATCCCCGTGTTACTGCCTATCAGCATTTTACTACTTTAAAAAAAAAAAAAAAGCCAAAAACCAAATTTGTATTTAAGGTATATAAAT TTTCCCAAAACTGATACCCTTTGAAAAAGTATAAATAAAATGAGCAAAAGTTGATCAGAGTGGGAAAGTAGTTCTTTTCAATCTAGAAAAGGCCAAAGTA ATGATTGAGATACACTGTCTCCACTTGCTTTGATTTTGTTGTTTCATTTTATAAAAGGTAGAAAAAATTTTGGAAATGTCATTGTCAGTTATTTGGCCTG CAGCACTGTCTTGGGGTGAATGGATGTAGCCTTCATGTAAAAACACTGTGTGGAGCAGCTTTATCTGCATTCAAACCTCAAGGTAGGGATGAGGGACTCC CCAGACATTTCTCTGGTGCTTTTCTGTCCAGGGTAAGCCACGAGGCATTGTCATCTCAGGGTAGTGCACCGCACATGCTCACACCTAGGCTTACCCAGGC AGAAGCGGCACAGATTCCAGTCTGGCTATTGCTTATCACATCCCTGGAGTGTGAAACATTTCCCAAGGGGCTGGGCTGGGGACATGAGCCTCCCATGAGC TGTACTTTTGCAAATGTATTTTACAAGTGTATGCACTTACCCAAAATAAAAATATAACGTGATAATTCAATTACAGACAAAAGATAAAGTGTGTAAG

STAT1.u.f.1 (forward primer)
STAT1.u.r.5 (reverse primer)
Multiple exon skipping

Frame 0

GGGGRGPGASAAFSPAG*FRFPAQSLRRGSAAP GGSRLADPRPSRGDPARSGEAAPPRPRLALPDPA RRKVSFAVCHPRELSRLTFALCVYNLDSLGT*R AVRSCSFG*IPRPLLGHKVAGIHQCTESSTNIH PFKW*KQTIMKIKYVDVSVVRTSAA*LKIPGAGS PAL**QFSHGNQTVPGTVVRKARLGARCQ*CFI CHHPFS*PPVTAG*SI*SLFFGE*LLATA*HKE KQA*SSG*FSGRPNPDVYDHLQLSEGRKENSGKR PEI*SGSVGEYSEHSDVRQTERA*Q*SQKCEGQ GYVYRA*NQEPGRFTR*I*LQMQNLAEQRTRDQ WCGKE*SETRTAVTQEDVFNA*Q*EKGSSSQNNR VAECH*TYPECPD***TSGVEAETAERLYWGAA QCLLGSAAELVHYSCGESAASSAAA*KVGGIGT EIHLRT*PYHKKQTSVMGPHLQSFPAAHSELVCG GKTALHANAPSEAAGLEDRGPVHCEVETVGEIA RAEL*FESQSLI**RCE*EKYSKRI*EVQHFGH AHKSDEHGGVHQWQSGG*ISAPAIERTEKCWHQN E*GSSHRY*RASLP*F*NPIVPAWFGN*PRDDL SARCGDLQRQPAPERLGLHPLVQHAGGGTQESV LLPDSTMCTMGSAFRSAELAVFFCHQKRSQCGPA EHVGREASWS*RQPRWSHSVDEVL*GKYK**KF SLLALD*KHPRTH*KTPAPSLE*WVHHGLHQQG ARACPVEGPAAGDLPAAVQ*ELPGRGHHIHMGGA VPERRRT*LPCG*TLHEERTFCCYFP*HHSQLQ SHGC*EYS*ESPEVSVSKY*QRPCLWKVLLQAK GSTRANGT*WP*RNWIYQD*VDFCV*SVSEHRRV TCLQTSSQPCSWLGPVEDACILLFHCNCYRHHS *TC*DPRVTAYQHFTTLKKKKKPKTKFVFKVYK FSQN*YPLKKYK*NEQKLIRVGK*FFSI*KRPK* *LRYTVSTCFDFVVSFYKR*KKFWKCHCQLFGL QHCLGVNGCSLHVKTLCGAALSAFKPQGRDEGL PRHFSGAFLSRVSHEALSSQGSAPHMLTPRLTQA EAAQIPVWLLLITSLECETFPKGLGWGHEPPMS CTFANVFYKCMHLPKIKI*RDNSITDKR*SV*

Frame 1

VGAEGGRAPALPFLLPGSFAFLRRVCGGARLHR GDRAWQTPDRAEATQRARERLHRRAPA*PFRIL RAEKFHLLYAILESCLG*RSHSVYITSTVLAPNV LCVAAPLVESPGPCWGTRWQGYTSAQNPPPTSI PSSGENRQ**K*NMWMSQWYELQQLDSKFLEQV HQLYDDSFPMEIRQYLAQWLEKQDWEHAANDVSF ATIRFHDLLSQLDDQYSRFSLENNFLLQHNIRK SKRNLQDNFQEDPIQMSMIIYSCLKEERKILEN AQRFNQAQSGNIQSTVMLDKQKELDSKVRNVKDK VMCIEHEIKSLEDLQDEYDFKCKTLQNREHETN GVAKSDQKQEQLLLKKMYLMLDNKRKEVVHKII ELLNVTELTQNALINDELVEWKRRQQSACIGGPP NACLDQLQNWFTIVAESLQQVRQQLKKLEELEQ KYTYEHDPITKNKQVLWDRTFSLFQQLIQSSFV VERQPCMPTHPQRPLVLKTGVQFTVKLRLLVKLQ ELNYNLKVKVLFDKDVNERNTVKGFRKFNILGT HTKVMNMEESTNGSLAAEFRHLQLKEQKNAGTR TNEGPLIVTEELHSLSFETQLCQPGLVIDLETTS LPVVVISNVSQLPSGWASILWYNMLVAEPRNLS FFLTPPCARWAQLSEVLSWQFSSVTKRGLNVDQ LNMLGEKLLGPNASPDGLIPWTRFCKENINDKNF PFWLWIESILELIKKHLLPLWNDGCIMGFISKE RERALLKDQQPGTFLLRFSESSREGAITFTWVE RSQNGGEPDFHAVEPYTKKELSAVTFPDIIRNYK VMAAENIPENPLKYLYPNIDKDHAFGKYYSRPK EAPEPMELDGPKGTGYIKTELISVSEV*VNTEE *HVYKPQASLAPGWGLLKMLVFYFSIVIAIAITA ELVEIPVLLPISILLL*KKKKSQKPNLYLRYIN FPKTDTL*KSINKMSKS*SEWESSSFQSRKGQS ND*DTLSPLALILLFHFIKGRKNFGNVIVSYLAC STVLG*MDVAFM*KHCVEQLYLHSNLKVGMRDS PDISLVLFCPG*ATRHCHLRVVHRTCSHLGLPR QKRHRFQSGYCLSHPWSVKHFPRGWAGDMSLP*A VLLQMYFTSVCTYPK*KYNVIIQLQTKDKVCK

Frame 2

WGRKGAGRQRCLFSCRVVSLSCAESAEGLGCTG GIAPGRPQTEQRRPSALGRGCTAAPPPSPSGSC AQKSFICCMPSSRAV*VNVRTLCI*PRQSWHLT CCA*LLLWLNPQALVGAQGGRDTPVHRILHQHPS LQVVKTDNNENKICGCLSGTNFSSLTQNSWSRF TSFMMTVFPWKSDSTWHSG*KSKTGSTLPMMFH LPPSVFMTSCHSWMINIVAFLWRITSCYSIT*GK ASVIFRIIFRKTQSRCL*SFTAV*RKKGKFWKT PRDLIRLSRGIFRAQ*C*TNRKSLTVKSEM*RT RLCV*SMKSRAWKIYKMNMTSNAKPCRTENTRPM VWQRVIRNKNSCYSRRCI*CLTIRERK*FTK** SC*MSLNLPRMP*LMMN*WSGSGDSRAPVLGGR PMLAWISCRTGSL*LRRVCSKFGSSLKSWRNWNR NTPTNMTLSQKTNKCYGTAPSVFSSSSFRARLW WKDSPACQRTLRGRWS*RQGSSSL*S*DCW*NC KS*III*KSKSYLIKM*MREIQ*KDLGSSTFWAR TQK**TWRSPPMAVWRLNFGTCN*KNRKMLAPE RMRVLSSLLKSFTPLVLKPNCASLVW*LTSRRP LCPLW*SPTSASSRAVGPPSFGTTCWWRNPGICP SS*LHHVHDGLSFQKC*VGSFLLSPKEVSMWTS *TCWERSFLVLTPAPMVSFRGRGFVRKI*MIKI FPSGFGLKAS*NSLKNTCSLSGMMGASWASSARS ESVPC*RTSSRGPSCCGSVRAPGKGPSHSHGWS GPRTEANLTSMRLNPTRRKNFLLLLSLTSFAIT KSWLLRIFLRIP*SICIQILTKTMPLESITPGQR KHQSQWNLMALKELDISRLS*FLCLKCK*TQKS DMFTNLKPALLLAGAC*RCLYFTFPL*LLSPSQ LNLLRSPCYCLSAFYYFKKKKKAKNQICI*GI*I FPKLIPFEKV*IK*AKVDQSGKVVLFNLEKAKV MIEIHCLHLL*FCCFIL*KVEKILEMSLSVIWP AALSWGEWM*PSCKNTVWSSFICIQTSR*G*GTP QTFLWCFSVQGKPRGIVISG*CTAHAHT*AYPG RSGTDSSLAIAYHIPGV*NISQGAGLGT*ASHE LYFCKCILQVYALTQNKNIT**FNYRQKIKCV