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Sequence STAT1.fAug05
GGTGGGGGCGGAAGGGGGCCGGGCGCCAGCGCTGCCTTTTCTCCTGCCGGGTAGTTTCGCTTTCCTGCGCAGAGTCTGCGGAGGGGCTCGGCTGCACCGGGGGGATCGCGCCTGGCAGACCCCAGACCGAGCAGAGGCGACCCAGCGCGCTCGGGAGAGGCTGCACCGCCGCGCCCCCGCCTAGCCCTTCCGGATCCTGC
GCGCAGAAAAGTTTCATTTGCTGTATGCCATCCTCGAGAGCTGTCTAGGTTAACGTTCGCACTCTGTGTATATAACCTCGACAGTCTTGGCACCTAACGT
GCTGTGCGTAGCTGCTCCTTTGGTTGAATCCCCAGGCCCTTGTTGGGGCACAAGGTGGCAGGGATACACCAGTGCACAGAATCCTCCACCAACATCCATC
CCTTCAAGTGGTGAAAACAGACAATAATGAAAATAAAATATGATGTCTCAGTGGTACGAACTTCAGCAGCTTGACTCAAAATTCCTGGAGCAGGTTCACC
AGCTTTATGATGACAGTTTTCCCATGGAAATCAGACAGTACCTGGCACAGTGGTTAGAAAAGCAAGACTGGGAGCACGCTGCCAATGATGTTTCATTTGC
CACCATCCGTTTTCATGACCTCCTGTCACAGCTGGATGATCAATATAGTCGCTTTTCTTTGGAGAATAACTTCTTGCTACAGCATAACATAAGGAAAAGC
AAGCGTAATCTTCAG
- STAT1.u.f.1 (forward primer)
- STAT1.u.r.5 (reverse primer)
- Alternative donor
Frame 0
GGGGRGPGASAAFSPAG*FRFPAQSLRRGSAAPGGSRLADPRPSRGDPARSGEAAPPRPRLALPDPA
RRKVSFAVCHPRELSRLTFALCVYNLDSLGT*R
AVRSCSFG*IPRPLLGHKVAGIHQCTESSTNIH
PFKW*KQTIMKIKYDVSVVRTSAA*LKIPGAGSP
AL**QFSHGNQTVPGTVVRKARLGARCQ*CFIC
HHPFS*PPVTAG*SI*SLFFGE*LLATA*HKEK
QA*SS
Frame 1
VGAEGGRAPALPFLLPGSFAFLRRVCGGARLHRGDRAWQTPDRAEATQRARERLHRRAPA*PFRIL
RAEKFHLLYAILESCLG*RSHSVYITSTVLAPNV
LCVAAPLVESPGPCWGTRWQGYTSAQNPPPTSI
PSSGENRQ**K*NMMSQWYELQQLDSKFLEQVH
QLYDDSFPMEIRQYLAQWLEKQDWEHAANDVSFA
TIRFHDLLSQLDDQYSRFSLENNFLLQHNIRKS
KRNLQ
Frame 2
WGRKGAGRQRCLFSCRVVSLSCAESAEGLGCTGGIAPGRPQTEQRRPSALGRGCTAAPPPSPSGSC
AQKSFICCMPSSRAV*VNVRTLCI*PRQSWHLT
CCA*LLLWLNPQALVGAQGGRDTPVHRILHQHPS
LQVVKTDNNENKI*CLSGTNFSSLTQNSWSRFT
SFMMTVFPWKSDSTWHSG*KSKTGSTLPMMFHL
PPSVFMTSCHSWMINIVAFLWRITSCYSIT*GKA
SVIF
Sequence STAT1.dAug05
GGTGGGGGCGGAAGGGGGCCGGGCGCCAGCGCTGCCTTTTCTCCTGCCGGGTAGTTTCGCTTTCCTGCGCAGAGTCTGCGGAGGGGCTCGGCTGCACCGGGGGGATCGCGCCTGGCAGACCCCAGACCGAGCAGAGGCGACCCAGCGCGCTCGGGAGAGGCTGCACCGCCGCGCCCCCGCCTAGCCCTTCCGGATCCTGC
GCGCAGAAAAGTTTCATTTGCTGTATGCCATCCTCGAGAGCTGTCTAGGTTAACGTTCGCACTCTGTGTATATAACCTCGACAGTCTTGGCACCTAACGT
GCTGTGCGTAGCTGCTCCTTTGGTTGAATCCCCAGGCCCTTGTTGGGGCACAAGGTGGCAGGGATACACCAGTGCACAGAATCCTCCACCAACATCCATC
CCTTCAAGTGGTGAAAACAGACAATAATGAAAATAAAATATGTGGATGTCTCAGTGGTACGAACTTCAGCAGCTTGACTCAAAATTCCTGGAGCAGGTTC
ACCAGCTTTATGATGACAGTTTTCCCATGGAAATCAGACAGTACCTGGCACAGTGGTTAGAAAAGCAAGACTGGGAGCACGCTGCCAATGATGTTTCATT
TGCCACCATCCGTTTTCATGACCTCCTGTCACAGCTGGATGATCAATATAGTCGCTTTTCTTTGGAGAATAACTTCTTGCTACAGCATAACATAAGGAAA
AGCAAGCGTAATCTTCAGGATAATTTTCAGGAAGACCCAATCCAGATGTCTATGATCATTTACAGCTGTCTGAAGGAAGAAAGGAAAATTCTGGAAAACG
CCCAGAGATTTAATCAGGCTCAGTCGGGGAATATTCAGAGCACAGTGATGTTAGACAAACAGAAAGAGCTTGACAGTAAAGTCAGAAATGTGAAGGACAA
GGTTATGTGTATAGAGCATGAAATCAAGAGCCTGGAAGATTTACAAGATGAATATGACTTCAAATGCAAAACCTTGCAGAACAGAGAACACGAGACCAAT
GGTGTGGCAAAGAGTGATCAGAAACAAGAACAGCTGTTACTCAAGAAGATGTATTTAATGCTTGACAATAAGAGAAAGGAAGTAGTTCACAAAATAATAG
AGTTGCTGAATGTCACTGAACTTACCCAGAATGCCCTGATTAATGATGAACTAGTGGAGTGGAAGCGGAGACAGCAGAGCGCCTGTATTGGGGGGCCGCC
CAATGCTTGCTTGGATCAGCTGCAGAACTGGTTCACTATAGTTGCGGAGAGTCTGCAGCAAGTTCGGCAGCAGCTTAAAAAGTTGGAGGAATTGGAACAG
AAATACACCTACGAACATGACCCTATCACAAAAAACAAACAAGTGTTATGGGACCGCACCTTCAGTCTTTTCCAGCAGCTCATTCAGAGCTCGTTTGTGG
TGGAAAGACAGCCCTGCATGCCAACGCACCCTCAGAGGCCGCTGGTCTTGAAGACAGGGGTCCAGTTCACTGTGAAGTTGAGACTGTTGGTGAAATTGCA
AGAGCTGAATTATAATTTGAAAGTCAAAGTCTTATTTGATAAAGATGTGAATGAGAGAAATACAGTAAAAGGATTTAGGAAGTTCAACATTTTGGGCACG
CACACAAAAGTGATGAACATGGAGGAGTCCACCAATGGCAGTCTGGCGGCTGAATTTCGGCACCTGCAATTGAAAGAACAGAAAAATGCTGGCACCAGAA
CGAATGAGGGTCCTCTCATCGTTACTGAAGAGCTTCACTCCCTTAGTTTTGAAACCCAATTGTGCCAGCCTGGTTTGGTAATTGACCTCGAGACGACCTC
TCTGCCCGTTGTGGTGATCTCCAACGTCAGCCAGCTCCCGAGCGGTTGGGCCTCCATCCTTTGGTACAACATGCTGGTGGCGGAACCCAGGAATCTGTCC
TTCTTCCTGACTCCACCATGTGCACGATGGGCTCAGCTTTCAGAAGTGCTGAGTTGGCAGTTTTCTTCTGTCACCAAAAGAGGTCTCAATGTGGACCAGC
TGAACATGTTGGGAGAGAAGCTTCTTGGTCCTAACGCCAGCCCCGATGGTCTCATTCCGTGGACGAGGTTTTGTAAGGAAAATATAAATGATAAAAATTT
TCCCTTCTGGCTTTGGATTGAAAGCATCCTAGAACTCATTAAAAAACACCTGCTCCCTCTCTGGAATGATGGGTGCATCATGGGCTTCATCAGCAAGGAG
CGAGAGCGTGCCCTGTTGAAGGACCAGCAGCCGGGGACCTTCCTGCTGCGGTTCAGTGAGAGCTCCCGGGAAGGGGCCATCACATTCACATGGGTGGAGC
GGTCCCAGAACGGAGGCGAACCTGACTTCCATGCGGTTGAACCCTACACGAAGAAAGAACTTTCTGCTGTTACTTTCCCTGACATCATTCGCAATTACAA
AGTCATGGCTGCTGAGAATATTCCTGAGAATCCCCTGAAGTATCTGTATCCAAATATTGACAAAGACCATGCCTTTGGAAAGTATTACTCCAGGCCAAAG
GAAGCACCAGAGCCAATGGAACTTGATGGCCCTAAAGGAACTGGATATATCAAGACTGAGTTGATTTCTGTGTCTGAAGTGTAAGTGAACACAGAAGAGT
GACATGTTTACAAACCTCAAGCCAGCCTTGCTCCTGGCTGGGGCCTGTTGAAGATGCTTGTATTTTACTTTTCCATTGTAATTGCTATCGCCATCACAGC
TGAACTTGTTGAGATCCCCGTGTTACTGCCTATCAGCATTTTACTACTTTAAAAAAAAAAAAAAAGCCAAAAACCAAATTTGTATTTAAGGTATATAAAT
TTTCCCAAAACTGATACCCTTTGAAAAAGTATAAATAAAATGAGCAAAAGTTGATCAGAGTGGGAAAGTAGTTCTTTTCAATCTAGAAAAGGCCAAAGTA
ATGATTGAGATACACTGTCTCCACTTGCTTTGATTTTGTTGTTTCATTTTATAAAAGGTAGAAAAAATTTTGGAAATGTCATTGTCAGTTATTTGGCCTG
CAGCACTGTCTTGGGGTGAATGGATGTAGCCTTCATGTAAAAACACTGTGTGGAGCAGCTTTATCTGCATTCAAACCTCAAGGTAGGGATGAGGGACTCC
CCAGACATTTCTCTGGTGCTTTTCTGTCCAGGGTAAGCCACGAGGCATTGTCATCTCAGGGTAGTGCACCGCACATGCTCACACCTAGGCTTACCCAGGC
AGAAGCGGCACAGATTCCAGTCTGGCTATTGCTTATCACATCCCTGGAGTGTGAAACATTTCCCAAGGGGCTGGGCTGGGGACATGAGCCTCCCATGAGC
TGTACTTTTGCAAATGTATTTTACAAGTGTATGCACTTACCCAAAATAAAAATATAACGTGATAATTCAATTACAGACAAAAGATAAAGTGTGTAAG
- STAT1.u.f.1 (forward primer)
- STAT1.u.r.5 (reverse primer)
- Alternative donor
Frame 0
GGGGRGPGASAAFSPAG*FRFPAQSLRRGSAAPGGSRLADPRPSRGDPARSGEAAPPRPRLALPDPA
RRKVSFAVCHPRELSRLTFALCVYNLDSLGT*R
AVRSCSFG*IPRPLLGHKVAGIHQCTESSTNIH
PFKW*KQTIMKIKYVDVSVVRTSAA*LKIPGAGS
PAL**QFSHGNQTVPGTVVRKARLGARCQ*CFI
CHHPFS*PPVTAG*SI*SLFFGE*LLATA*HKE
KQA*SSG*FSGRPNPDVYDHLQLSEGRKENSGKR
PEI*SGSVGEYSEHSDVRQTERA*Q*SQKCEGQ
GYVYRA*NQEPGRFTR*I*LQMQNLAEQRTRDQ
WCGKE*SETRTAVTQEDVFNA*Q*EKGSSSQNNR
VAECH*TYPECPD***TSGVEAETAERLYWGAA
QCLLGSAAELVHYSCGESAASSAAA*KVGGIGT
EIHLRT*PYHKKQTSVMGPHLQSFPAAHSELVCG
GKTALHANAPSEAAGLEDRGPVHCEVETVGEIA
RAEL*FESQSLI**RCE*EKYSKRI*EVQHFGH
AHKSDEHGGVHQWQSGG*ISAPAIERTEKCWHQN
E*GSSHRY*RASLP*F*NPIVPAWFGN*PRDDL
SARCGDLQRQPAPERLGLHPLVQHAGGGTQESV
LLPDSTMCTMGSAFRSAELAVFFCHQKRSQCGPA
EHVGREASWS*RQPRWSHSVDEVL*GKYK**KF
SLLALD*KHPRTH*KTPAPSLE*WVHHGLHQQG
ARACPVEGPAAGDLPAAVQ*ELPGRGHHIHMGGA
VPERRRT*LPCG*TLHEERTFCCYFP*HHSQLQ
SHGC*EYS*ESPEVSVSKY*QRPCLWKVLLQAK
GSTRANGT*WP*RNWIYQD*VDFCV*SVSEHRRV
TCLQTSSQPCSWLGPVEDACILLFHCNCYRHHS
*TC*DPRVTAYQHFTTLKKKKKPKTKFVFKVYK
FSQN*YPLKKYK*NEQKLIRVGK*FFSI*KRPK*
*LRYTVSTCFDFVVSFYKR*KKFWKCHCQLFGL
QHCLGVNGCSLHVKTLCGAALSAFKPQGRDEGL
PRHFSGAFLSRVSHEALSSQGSAPHMLTPRLTQA
EAAQIPVWLLLITSLECETFPKGLGWGHEPPMS
CTFANVFYKCMHLPKIKI*RDNSITDKR*SV*
Frame 1
VGAEGGRAPALPFLLPGSFAFLRRVCGGARLHRGDRAWQTPDRAEATQRARERLHRRAPA*PFRIL
RAEKFHLLYAILESCLG*RSHSVYITSTVLAPNV
LCVAAPLVESPGPCWGTRWQGYTSAQNPPPTSI
PSSGENRQ**K*NMWMSQWYELQQLDSKFLEQV
HQLYDDSFPMEIRQYLAQWLEKQDWEHAANDVSF
ATIRFHDLLSQLDDQYSRFSLENNFLLQHNIRK
SKRNLQDNFQEDPIQMSMIIYSCLKEERKILEN
AQRFNQAQSGNIQSTVMLDKQKELDSKVRNVKDK
VMCIEHEIKSLEDLQDEYDFKCKTLQNREHETN
GVAKSDQKQEQLLLKKMYLMLDNKRKEVVHKII
ELLNVTELTQNALINDELVEWKRRQQSACIGGPP
NACLDQLQNWFTIVAESLQQVRQQLKKLEELEQ
KYTYEHDPITKNKQVLWDRTFSLFQQLIQSSFV
VERQPCMPTHPQRPLVLKTGVQFTVKLRLLVKLQ
ELNYNLKVKVLFDKDVNERNTVKGFRKFNILGT
HTKVMNMEESTNGSLAAEFRHLQLKEQKNAGTR
TNEGPLIVTEELHSLSFETQLCQPGLVIDLETTS
LPVVVISNVSQLPSGWASILWYNMLVAEPRNLS
FFLTPPCARWAQLSEVLSWQFSSVTKRGLNVDQ
LNMLGEKLLGPNASPDGLIPWTRFCKENINDKNF
PFWLWIESILELIKKHLLPLWNDGCIMGFISKE
RERALLKDQQPGTFLLRFSESSREGAITFTWVE
RSQNGGEPDFHAVEPYTKKELSAVTFPDIIRNYK
VMAAENIPENPLKYLYPNIDKDHAFGKYYSRPK
EAPEPMELDGPKGTGYIKTELISVSEV*VNTEE
*HVYKPQASLAPGWGLLKMLVFYFSIVIAIAITA
ELVEIPVLLPISILLL*KKKKSQKPNLYLRYIN
FPKTDTL*KSINKMSKS*SEWESSSFQSRKGQS
ND*DTLSPLALILLFHFIKGRKNFGNVIVSYLAC
STVLG*MDVAFM*KHCVEQLYLHSNLKVGMRDS
PDISLVLFCPG*ATRHCHLRVVHRTCSHLGLPR
QKRHRFQSGYCLSHPWSVKHFPRGWAGDMSLP*A
VLLQMYFTSVCTYPK*KYNVIIQLQTKDKVCK
Frame 2
WGRKGAGRQRCLFSCRVVSLSCAESAEGLGCTGGIAPGRPQTEQRRPSALGRGCTAAPPPSPSGSC
AQKSFICCMPSSRAV*VNVRTLCI*PRQSWHLT
CCA*LLLWLNPQALVGAQGGRDTPVHRILHQHPS
LQVVKTDNNENKICGCLSGTNFSSLTQNSWSRF
TSFMMTVFPWKSDSTWHSG*KSKTGSTLPMMFH
LPPSVFMTSCHSWMINIVAFLWRITSCYSIT*GK
ASVIFRIIFRKTQSRCL*SFTAV*RKKGKFWKT
PRDLIRLSRGIFRAQ*C*TNRKSLTVKSEM*RT
RLCV*SMKSRAWKIYKMNMTSNAKPCRTENTRPM
VWQRVIRNKNSCYSRRCI*CLTIRERK*FTK**
SC*MSLNLPRMP*LMMN*WSGSGDSRAPVLGGR
PMLAWISCRTGSL*LRRVCSKFGSSLKSWRNWNR
NTPTNMTLSQKTNKCYGTAPSVFSSSSFRARLW
WKDSPACQRTLRGRWS*RQGSSSL*S*DCW*NC
KS*III*KSKSYLIKM*MREIQ*KDLGSSTFWAR
TQK**TWRSPPMAVWRLNFGTCN*KNRKMLAPE
RMRVLSSLLKSFTPLVLKPNCASLVW*LTSRRP
LCPLW*SPTSASSRAVGPPSFGTTCWWRNPGICP
SS*LHHVHDGLSFQKC*VGSFLLSPKEVSMWTS
*TCWERSFLVLTPAPMVSFRGRGFVRKI*MIKI
FPSGFGLKAS*NSLKNTCSLSGMMGASWASSARS
ESVPC*RTSSRGPSCCGSVRAPGKGPSHSHGWS
GPRTEANLTSMRLNPTRRKNFLLLLSLTSFAIT
KSWLLRIFLRIP*SICIQILTKTMPLESITPGQR
KHQSQWNLMALKELDISRLS*FLCLKCK*TQKS
DMFTNLKPALLLAGAC*RCLYFTFPL*LLSPSQ
LNLLRSPCYCLSAFYYFKKKKKAKNQICI*GI*I
FPKLIPFEKV*IK*AKVDQSGKVVLFNLEKAKV
MIEIHCLHLL*FCCFIL*KVEKILEMSLSVIWP
AALSWGEWM*PSCKNTVWSSFICIQTSR*G*GTP
QTFLWCFSVQGKPRGIVISG*CTAHAHT*AYPG
RSGTDSSLAIAYHIPGV*NISQGAGLGT*ASHE
LYFCKCILQVYALTQNKNIT**FNYRQKIKCV
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