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Sequences

Sequence STAT1.cAug05

GGTGGGGGCGGAAGGGGGCCGGGCGCCAGCGCTGCCTTTTCTCCTGCCGGGTAGTTTCGCTTTCCTGCGCAGAGTCTGCGGAGGGGCTCGGCTGCACCGG GGGGATCGCGCCTGGCAGACCCCAGACCGAGCAGAGGCGACCCAGCGCGCTCGGGAGAGGCTGCACCGCCGCGCCCCCGCCTAGCCCTTCCGGATCCTGC GCGCAGAAAAGTTTCATTTGCTGTATGCCATCCTCGAGAGCTGTCTAGGTTAACGTTCGCACTCTGTGTATATAACCTCGACAGTCTTGGCACCTAACGT GCTGTGCGTAGCTGCTCCTTTGGTTGAATCCCCAGGCCCTTGTTGGGGCACAAGGTGGCAGGATGTCTCAGTGGTACGAACTTCAGCAGCTTGACTCAAA ATTCCTGGAGCAGGTTCACCAGCTTTATGATGACAGTTTTCCCATGGAAATCAGACAGTACCTGGCACAGTGGTTAGAAAAGCAAGACTGGGAGCACGCT GCCAATGATGTTTCATTTGCCACCATCCGTTTTCATGACCTCCTGTCACAGCTGGATGATCAATATAGTCGCTTTTCTTTGGAGAATAACTTCTTGCTAC AGCATAACATAAGGAAAAGCAAGCGTAATCTTCAGGATAATTTTCAGGAAGACCCAATCCAGATGTCTATGATCATTTACAGCTGTCTGAAGGAAGAAAG GAAAATTCTGGAAAACGCCCAGAGATTTAATCAGGCTCAGTCGGGGAATATTCAGAGCACAGTGATGTTAGACAAACAGAAAGAGCTTGACAGTAAAGTC AGAAATGTGAAGGACAAGGTTATGTGTATAGAGCATGAAATCAAGAGCCTGGAAGATTTACAAGATGAATATGACTTCAAATGCAAAACCTTGCAGAACA GAGAACACGAGACCAATGGTGTGGCAAAGAGTGATCAGAAACAAGAACAGCTGTTACTCAAGAAGATGTATTTAATGCTTGACAATAAGAGAAAGGAAGT AGTTCACAAAATAATAGAGTTGCTGAATGTCACTGAACTTACCCAGAATGCCCTGATTAATGATGAACTAGTGGAGTGGAAGCGGAGACAGCAGAGCGCC TGTATTGGGGGGCCGCCCAATGCTTGCTTGGATCAGCTGCAGAACTGGTTCACTATAGTTGCGGAGAGTCTGCAGCAAGTTCGGCAGCAGCTTAAAAAGT TGGAGGAATTGGAACAGAAATACACCTACGAACATGACCCTATCACAAAAAACAAACAAGTGTTATGGGACCGCACCTTCAGTCTTTTCCAGCAGCTCAT TCAGAGCTCGTTTGTGGTGGAAAGACAGCCCTGCATGCCAACGCACCCTCAGAGGCCGCTGGTCTTGAAGACAGGGGTCCAGTTCACTGTGAAGTTGAGA CTGTTGGTGAAATTGCAAGAGCTGAATTATAATTTGAAAGTCAAAGTCTTATTTGATAAAGATGTGAATGAGAGAAATACAGTAAAAGGATTTAGGAAGT TCAACATTTTGGGCACGCACACAAAAGTGATGAACATGGAGGAGTCCACCAATGGCAGTCTGGCGGCTGAATTTCGGCACCTGCAATTGAAAGAACAGAA AAATGCTGGCACCAGAACGAATGAGGGTCCTCTCATCGTTACTGAAGAGCTTCACTCCCTTAGTTTTGAAACCCAATTGTGCCAGCCTGGTTTGGTAATT GACCTCGAGACGACCTCTCTGCCCGTTGTGGTGATCTCCAACGTCAGCCAGCTCCCGAGCGGTTGGGCCTCCATCCTTTGGTACAACATGCTGGTGGCGG AACCCAGGAATCTGTCCTTCTTCCTGACTCCACCATGTGCACGATGGGCTCAGCTTTCAGAAGTGCTGAGTTGGCAGTTTTCTTCTGTCACCAAAAGAGG TCTCAATGTGGACCAGCTGAACATGTTGGGAGAGAAGCTTCTTGGTCCTAACGCCAGCCCCGATGGTCTCATTCCGTGGACGAGGTTTTGTAAGGAAAAT ATAAATGATAAAAATTTTCCCTTCTGGCTTTGGATTGAAAGCATCCTAGAACTCATTAAAAAACACCTGCTCCCTCTCTGGAATGATGGGTGCATCATGG GCTTCATCAGCAAGGAGCGAGAGCGTGCCCTGTTGAAGGACCAGCAGCCGGGGACCTTCCTGCTGCGGTTCAGTGAGAGCTCCCGGGAAGGGGCCATCAC ATTCACATGGGTGGAGCGGTCCCAGAACGGAGGCGAACCTGACTTCCATGCGGTTGAACCCTACACGAAGAAAGAACTTTCTGCTGTTACTTTCCCTGAC ATCATTCGCAATTACAAAGTCATGGCTGCTGAGAATATTCCTGAGAATCCCCTGAAGTATCTGTATCCAAATATTGACAAAGACCATGCCTTTGGAAAGT ATTACTCCAGGCCAAAGGAAGCACCAGAGCCAATGGAACTTGATGGCCCTAAAGGAACTGGATATATCAAGACTGAGTTGATTTCTGTGTCTGAAGTGTA AGTGAACACAGAAGAGTGACATGTTTACAAACCTCAAGCCAGCCTTGCTCCTGGCTGGGGCCTGTTGAAGATGCTTGTATTTTACTTTTCCATTGTAATT GCTATCGCCATCACAGCTGAACTTGTTGAGATCCCCGTGTTACTGCCTATCAGCATTTTACTACTTTAAAAAAAAAAAAAAAGCCAAAAACCAAATTTGT ATTTAAGGTATATAAATTTTCCCAAAACTGATACCCTTTGAAAAAGTATAAATAAAATGAGCAAAAGTTGATCAGAGTGGGAAAGTAGTTCTTTTCAATC TAGAAAAGGCCAAAGTAATGATTGAGATACACTGTCTCCACTTGCTTTGATTTTGTTGTTTCATTTTATAAAAGGTAGAAAAAATTTTGGAAATGTCATT GTCAGTTATTTGGCCTGCAGCACTGTCTTGGGGTGAATGGATGTAGCCTTCATGTAAAAACACTGTGTGGAGCAGCTTTATCTGCATTCAAACCTCAAGG TAGGGATGAGGGACTCCCCAGACATTTCTCTGGTGCTTTTCTGTCCAGGGTAAGCCACGAGGCATTGTCATCTCAGGGTAGTGCACCGCACATGCTCACA CCTAGGCTTACCCAGGCAGAAGCGGCACAGATTCCAGTCTGGCTATTGCTTATCACATCCCTGGAGTGTGAAACATTTCCCAAGGGGCTGGGCTGGGGAC ATGAGCCTCCCATGAGCTGTACTTTTGCAAATGTATTTTACAAGTGTATGCACTTACCCAAAATAAAAATATAACGTGATAATTCAATTACAGACAAAAG ATAAAGTGTGTAAG

STAT1.u.f.1 (forward primer)
STAT1.u.r.5 (reverse primer)
Single exon skipping

Frame 0

GGGGRGPGASAAFSPAG*FRFPAQSLRRGSAAP GGSRLADPRPSRGDPARSGEAAPPRPRLALPDPA RRKVSFAVCHPRELSRLTFALCVYNLDSLGT*R AVRSCSFG*IPRPLLGHKVAGCLSGTNFSSLTQ NSWSRFTSFMMTVFPWKSDSTWHSG*KSKTGSTL PMMFHLPPSVFMTSCHSWMINIVAFLWRITSCY SIT*GKASVIFRIIFRKTQSRCL*SFTAV*RKK GKFWKTPRDLIRLSRGIFRAQ*C*TNRKSLTVKS EM*RTRLCV*SMKSRAWKIYKMNMTSNAKPCRT ENTRPMVWQRVIRNKNSCYSRRCI*CLTIRERK *FTK**SC*MSLNLPRMP*LMMN*WSGSGDSRAP VLGGRPMLAWISCRTGSL*LRRVCSKFGSSLKS WRNWNRNTPTNMTLSQKTNKCYGTAPSVFSSSS FRARLWWKDSPACQRTLRGRWS*RQGSSSL*S*D CW*NCKS*III*KSKSYLIKM*MREIQ*KDLGS STFWARTQK**TWRSPPMAVWRLNFGTCN*KNR KMLAPERMRVLSSLLKSFTPLVLKPNCASLVW*L TSRRPLCPLW*SPTSASSRAVGPPSFGTTCWWR NPGICPSS*LHHVHDGLSFQKC*VGSFLLSPKE VSMWTS*TCWERSFLVLTPAPMVSFRGRGFVRKI *MIKIFPSGFGLKAS*NSLKNTCSLSGMMGASW ASSARSESVPC*RTSSRGPSCCGSVRAPGKGPS HSHGWSGPRTEANLTSMRLNPTRRKNFLLLLSLT SFAITKSWLLRIFLRIP*SICIQILTKTMPLES ITPGQRKHQSQWNLMALKELDISRLS*FLCLKC K*TQKSDMFTNLKPALLLAGAC*RCLYFTFPL*L LSPSQLNLLRSPCYCLSAFYYFKKKKKAKNQIC I*GI*IFPKLIPFEKV*IK*AKVDQSGKVVLFN LEKAKVMIEIHCLHLL*FCCFIL*KVEKILEMSL SVIWPAALSWGEWM*PSCKNTVWSSFICIQTSR *G*GTPQTFLWCFSVQGKPRGIVISG*CTAHAH T*AYPGRSGTDSSLAIAYHIPGV*NISQGAGLGT *ASHELYFCKCILQVYALTQNKNIT**FNYRQK IKCV

Frame 1

VGAEGGRAPALPFLLPGSFAFLRRVCGGARLHR GDRAWQTPDRAEATQRARERLHRRAPA*PFRIL RAEKFHLLYAILESCLG*RSHSVYITSTVLAPNV LCVAAPLVESPGPCWGTRWQDVSVVRTSAA*LK IPGAGSPAL**QFSHGNQTVPGTVVRKARLGAR CQ*CFICHHPFS*PPVTAG*SI*SLFFGE*LLAT A*HKEKQA*SSG*FSGRPNPDVYDHLQLSEGRK ENSGKRPEI*SGSVGEYSEHSDVRQTERA*Q*S QKCEGQGYVYRA*NQEPGRFTR*I*LQMQNLAEQ RTRDQWCGKE*SETRTAVTQEDVFNA*Q*EKGS SSQNNRVAECH*TYPECPD***TSGVEAETAER LYWGAAQCLLGSAAELVHYSCGESAASSAAA*KV GGIGTEIHLRT*PYHKKQTSVMGPHLQSFPAAH SELVCGGKTALHANAPSEAAGLEDRGPVHCEVE TVGEIARAEL*FESQSLI**RCE*EKYSKRI*EV QHFGHAHKSDEHGGVHQWQSGG*ISAPAIERTE KCWHQNE*GSSHRY*RASLP*F*NPIVPAWFGN *PRDDLSARCGDLQRQPAPERLGLHPLVQHAGGG TQESVLLPDSTMCTMGSAFRSAELAVFFCHQKR SQCGPAEHVGREASWS*RQPRWSHSVDEVL*GK YK**KFSLLALD*KHPRTH*KTPAPSLE*WVHHG LHQQGARACPVEGPAAGDLPAAVQ*ELPGRGHH IHMGGAVPERRRT*LPCG*TLHEERTFCCYFP* HHSQLQSHGC*EYS*ESPEVSVSKY*QRPCLWKV LLQAKGSTRANGT*WP*RNWIYQD*VDFCV*SV SEHRRVTCLQTSSQPCSWLGPVEDACILLFHCN CYRHHS*TC*DPRVTAYQHFTTLKKKKKPKTKFV FKVYKFSQN*YPLKKYK*NEQKLIRVGK*FFSI *KRPK**LRYTVSTCFDFVVSFYKR*KKFWKCH CQLFGLQHCLGVNGCSLHVKTLCGAALSAFKPQG RDEGLPRHFSGAFLSRVSHEALSSQGSAPHMLT PRLTQAEAAQIPVWLLLITSLECETFPKGLGWG HEPPMSCTFANVFYKCMHLPKIKI*RDNSITDKR *SV*

Frame 2

WGRKGAGRQRCLFSCRVVSLSCAESAEGLGCTG GIAPGRPQTEQRRPSALGRGCTAAPPPSPSGSC AQKSFICCMPSSRAV*VNVRTLCI*PRQSWHLT CCA*LLLWLNPQALVGAQGGRMSQWYELQQLDSK FLEQVHQLYDDSFPMEIRQYLAQWLEKQDWEHA ANDVSFATIRFHDLLSQLDDQYSRFSLENNFLL QHNIRKSKRNLQDNFQEDPIQMSMIIYSCLKEER KILENAQRFNQAQSGNIQSTVMLDKQKELDSKV RNVKDKVMCIEHEIKSLEDLQDEYDFKCKTLQN REHETNGVAKSDQKQEQLLLKKMYLMLDNKRKEV VHKIIELLNVTELTQNALINDELVEWKRRQQSA CIGGPPNACLDQLQNWFTIVAESLQQVRQQLKK LEELEQKYTYEHDPITKNKQVLWDRTFSLFQQLI QSSFVVERQPCMPTHPQRPLVLKTGVQFTVKLR LLVKLQELNYNLKVKVLFDKDVNERNTVKGFRK FNILGTHTKVMNMEESTNGSLAAEFRHLQLKEQK NAGTRTNEGPLIVTEELHSLSFETQLCQPGLVI DLETTSLPVVVISNVSQLPSGWASILWYNMLVA EPRNLSFFLTPPCARWAQLSEVLSWQFSSVTKRG LNVDQLNMLGEKLLGPNASPDGLIPWTRFCKEN INDKNFPFWLWIESILELIKKHLLPLWNDGCIM GFISKERERALLKDQQPGTFLLRFSESSREGAIT FTWVERSQNGGEPDFHAVEPYTKKELSAVTFPD IIRNYKVMAAENIPENPLKYLYPNIDKDHAFGK YYSRPKEAPEPMELDGPKGTGYIKTELISVSEV* VNTEE*HVYKPQASLAPGWGLLKMLVFYFSIVI AIAITAELVEIPVLLPISILLL*KKKKSQKPNL YLRYINFPKTDTL*KSINKMSKS*SEWESSSFQS RKGQSND*DTLSPLALILLFHFIKGRKNFGNVI VSYLACSTVLG*MDVAFM*KHCVEQLYLHSNLK VGMRDSPDISLVLFCPG*ATRHCHLRVVHRTCSH LGLPRQKRHRFQSGYCLSHPWSVKHFPRGWAGD MSLP*AVLLQMYFTSVCTYPK*KYNVIIQLQTK DKVCK

Sequence STAT1.aAug05

GGTGGGGGCGGAAGGGGGCCGGGCGCCAGCGCTGCCTTTTCTCCTGCCGGGTAGTTTCGCTTTCCTGCGCAGAGTCTGCGGAGGGGCTCGGCTGCACCGG GGGGATCGCGCCTGGCAGACCCCAGACCGAGCAGAGGCGACCCAGCGCGCTCGGGAGAGGCTGCACCGCCGCGCCCCCGCCTAGCCCTTCCGGATCCTGC GCGCAGAAAAGTTTCATTTGCTGTATGCCATCCTCGAGAGCTGTCTAGGTTAACGTTCGCACTCTGTGTATATAACCTCGACAGTCTTGGCACCTAACGT GCTGTGCGTAGCTGCTCCTTTGGTTGAATCCCCAGGCCCTTGTTGGGGCACAAGGTGGCAGGATGTCTCAGTGGTACGAACTTCAGCAGCTTGACTCAAA ATTCCTGGAGCAGGTTCACCAGCTTTATGATGACAGTTTTCCCATGGAAATCAGACAGTACCTGGCACAGTGGTTAGAAAAGCAAGACTGGGAGCACGCT GCCAATGATGTTTCATTTGCCACCATCCGTTTTCATGACCTCCTGTCACAGCTGGATGATCAATATAGTCGCTTTTCTTTGGAGAATAACTTCTTGCTAC AGCATAACATAAGGAAAAGCAAGCGTAATCTTCAGGATAATTTTCAGGAAGACCCAATCCAGATGTCTATGATCATTTACAGCTGTCTGAAGGAAGAAAG GAAAATTCTGGAAAACGCCCAGAGATTTAATCAGGCTCAGTCGGGGAATATTCAGAGCACAGTGATGTTAGACAAACAGAAAGAGCTTGACAGTAAAGTC AGAAATGTGAAGGACAAGGTTATGTGTATAGAGCATGAAATCAAGAGCCTGGAAGATTTACAAGATGAATATGACTTCAAATGCAAAACCTTGCAGAACA GAGAACACGAGACCAATGGTGTGGCAAAGAGTGATCAGAAACAAGAACAGCTGTTACTCAAGAAGATGTATTTAATGCTTGACAATAAGAGAAAGGAAGT AGTTCACAAAATAATAGAGTTGCTGAATGTCACTGAACTTACCCAGAATGCCCTGATTAATGATGAACTAGTGGAGTGGAAGCGGAGACAGCAGAGCGCC TGTATTGGGGGGCCGCCCAATGCTTGCTTGGATCAGCTGCAGAACTGGTTCACTATAGTTGCGGAGAGTCTGCAGCAAGTTCGGCAGCAGCTTAAAAAGT TGGAGGAATTGGAACAGAAATACACCTACGAACATGACCCTATCACAAAAAACAAACAAGTGTTATGGGACCGCACCTTCAGTCTTTTCCAGCAGCTCAT TCAGAGCTCGTTTGTGGTGGAAAGACAGCCCTGCATGCCAACGCACCCTCAGAGGCCGCTGGTCTTGAAGACAGGGGTCCAGTTCACTGTGAAGTTGAGA CTGTTGGTGAAATTGCAAGAGCTGAATTATAATTTGAAAGTCAAAGTCTTATTTGATAAAGATGTGAATGAGAGAAATACAGTAAAAGGATTTAGGAAGT TCAACATTTTGGGCACGCACACAAAAGTGATGAACATGGAGGAGTCCACCAATGGCAGTCTGGCGGCTGAATTTCGGCACCTGCAATTGAAAGAACAGAA AAATGCTGGCACCAGAACGAATGAGGGTCCTCTCATCGTTACTGAAGAGCTTCACTCCCTTAGTTTTGAAACCCAATTGTGCCAGCCTGGTTTGGTAATT GACCTCGAGACGACCTCTCTGCCCGTTGTGGTGATCTCCAACGTCAGCCAGCTCCCGAGCGGTTGGGCCTCCATCCTTTGGTACAACATGCTGGTGGCGG AACCCAGGAATCTGTCCTTCTTCCTGACTCCACCATGTGCACGATGGGCTCAGCTTTCAGAAGTGCTGAGTTGGCAGTTTTCTTCTGTCACCAAAAGAGG TCTCAATGTGGACCAGCTGAACATGTTGGGAGAGAAGCTTCTTGGTCCTAACGCCAGCCCCGATGGTCTCATTCCGTGGACGAGGTTTTGTAAGGAAAAT ATAAATGATAAAAATTTTCCCTTCTGGCTTTGGATTGAAAGCATCCTAGAACTCATTAAAAAACACCTGCTCCCTCTCTGGAATGATGGGTGCATCATGG GCTTCATCAGCAAGGAGCGAGAGCGTGCCCTGTTGAAGGACCAGCAGCCGGGGACCTTCCTGCTGCGGTTCAGTGAGAGCTCCCGGGAAGGGGCCATCAC ATTCACATGGGTGGAGCGGTCCCAGAACGGAGGCGAACCTGACTTCCATGCGGTTGAACCCTACACGAAGAAAGAACTTTCTGCTGTTACTTTCCCTGAC ATCATTCGCAATTACAAAGTCATGGCTGCTGAGAATATTCCTGAGAATCCCCTGAAGTATCTGTATCCAAATATTGACAAAGACCATGCCTTTGGAAAGT ATTACTCCAGGCCAAAGGAAGCACCAGAGCCAATGGAACTTGATGGCCCTAAAGGAACTGGATATATCAAGACTGAGTTGATTTCTGTGTCTGAAGTTCA CCCTTCTAGACTTCAGACCACAGACAACCTGCTCCCCATGTCTCCTGAGGAGTTTGACGAGGTGTCTCGGATAGTGGGCTCTGTAGAATTCGACAGTATG ATGAACACAGTATAGAGCATGAATTTTTTTCATCTTCTCTGGCGACAGTTTTCCTTCTCATCTGTGATTCCCTCCTGCTACTCTGTTCCTTCACATCCTG TGTTTCTAGGGAAATGAAAGAAAGGCCAGCAAATTCGCTGCAACCTGTTGATAGCAAGTGAATTTTTCTCTAACTCAGAAACATCAGTTACTCTGAAGGG CATCATGCATCTTACTGAAGGTAAAATTGAAAGGCATTCTCTGAAGAGTGGGTTTCACAAGTGAAAAACATCCAGATACACCCAAAGTATCAGGACGAGA ATGAGGGTCCTTTGGGAAAGGAGAAGTTAAGCAACATCTAGCAAATGTTATGCATAAAGTCAGTGCCCAACTGTTATAGGTTGTTGGATAAATCAGTGGT TATTTAGGGAACTGCTTGACGTAGGAACGGTAAATTTCTGTGGGAGAATTCTTACATGTTTTCTTTGCTTTAAGTGTAACTGGCAGTTTTCCATTGGTTT ACCTGTGAAATAGTTCAAAGCCAAGTTTATATACAATTATATCAGTCCTCTTTCAAAGGTAGCCATCATGGATCTGGTAGGGGGAAAATGTGTATTTTAT TACATCTTTCACATTGGCTATTTAAAGACAAAGACAAATTCTGTTTCTTGAGAAGAGAATATTAGCTTTACTGTTTGTTATGGCTTAATGACACTAGCTA ATATCAATAGAAGGATGTACATTTCCAAATTCACAAGTTGTGTTTGATATCCAAAGCTGAATACATTCTGCTTTCATCTTGGTCACATACAATTATTTTT ACAGTTCTCCCAAGGGAGTTAGGCTATTCACAACCACTCATTCAAAAGTTGAAATTAACCATAGATGTAGATAAACTCAGAAATTTAATTCATGTTTCTT AAATGGGCTACTTTGTCCTTTTTGTTATTAGGGTGGTATTTAGTCTATTAGCCACAAAATTGGGAAAGGAGTAGAAAAAGCAGTAACTGACAACTTGAAT AATACACCAGAGATAATATGAGAATCAGATCATTTCAAAACTCATTTCCTATGTAACTGCATTGAGAACTGCATATGTTTCGCTGATATATGTGTTTTTC ACATTTGCGAATGGTTCCATTCTCTCTCCTGTACTTTTTCCAGACACTTTTTTGAGTGGATGATGTTTCGTGAAGTATACTGTATTTTTACCTTTTTCCT TCCTTATCACTGACACAAAAAGTAGATTAAGAGATGGGTTTGACAAGGTTCTTCCCTTTTACATACTGCTGTCTATGTGGCTGTATCTTGTTTTTCCACT ACTGCTACCACAACTATATTATCATGCAAATGCTGTATTCTTCTTTGGTGGAGATAAAGATTTCTTGAGTTTTGTTTTAAAATTAAAGCTAAAGTATCTG TATTGCATTAAATATAATATGCACACAGTGCTTTCCGTGGCACTGCATACAATCTGAGGCCTCCTCTCTCAGTTTTTATATAGATGGCGAGAACCTAAGT TTCAGTTGATTTTACAATTGAAATGACTAAAAAACAAAGAAGACAACATTAAAACAATATTGTTTCTAATTGCTGAGGTTTAGCTGTCAGTTCTTTTTGC CCTTTGGGAATTCGGCATGGTTTCATTTTACTGCACTAGCCAAGAGACTTTACTTTTAAGAAGTATTAAAATTCTAAAATTCTATTAA

STAT1.u.f.1 (forward primer)
STAT1.u.r.5 (reverse primer)
Single exon skipping

Frame 0

GGGGRGPGASAAFSPAG*FRFPAQSLRRGSAAP GGSRLADPRPSRGDPARSGEAAPPRPRLALPDPA RRKVSFAVCHPRELSRLTFALCVYNLDSLGT*R AVRSCSFG*IPRPLLGHKVAGCLSGTNFSSLTQ NSWSRFTSFMMTVFPWKSDSTWHSG*KSKTGSTL PMMFHLPPSVFMTSCHSWMINIVAFLWRITSCY SIT*GKASVIFRIIFRKTQSRCL*SFTAV*RKK GKFWKTPRDLIRLSRGIFRAQ*C*TNRKSLTVKS EM*RTRLCV*SMKSRAWKIYKMNMTSNAKPCRT ENTRPMVWQRVIRNKNSCYSRRCI*CLTIRERK *FTK**SC*MSLNLPRMP*LMMN*WSGSGDSRAP VLGGRPMLAWISCRTGSL*LRRVCSKFGSSLKS WRNWNRNTPTNMTLSQKTNKCYGTAPSVFSSSS FRARLWWKDSPACQRTLRGRWS*RQGSSSL*S*D CW*NCKS*III*KSKSYLIKM*MREIQ*KDLGS STFWARTQK**TWRSPPMAVWRLNFGTCN*KNR KMLAPERMRVLSSLLKSFTPLVLKPNCASLVW*L TSRRPLCPLW*SPTSASSRAVGPPSFGTTCWWR NPGICPSS*LHHVHDGLSFQKC*VGSFLLSPKE VSMWTS*TCWERSFLVLTPAPMVSFRGRGFVRKI *MIKIFPSGFGLKAS*NSLKNTCSLSGMMGASW ASSARSESVPC*RTSSRGPSCCGSVRAPGKGPS HSHGWSGPRTEANLTSMRLNPTRRKNFLLLLSLT SFAITKSWLLRIFLRIP*SICIQILTKTMPLES ITPGQRKHQSQWNLMALKELDISRLS*FLCLKF TLLDFRPQTTCSPCLLRSLTRCLG*WAL*NSTV* *TQYRA*IFFIFSGDSFPSHL*FPPATLFLHIL CF*GNERKASKFAATC**QVNFSLTQKHQLL*R ASCILLKVKLKGIL*RVGFTSEKHPDTPKVSGRE *GSFGKGEVKQHLANVMHKVSAQLL*VVG*ISG YLGNCLT*ER*ISVGEFLHVFFALSVTGSFPLV YL*NSSKPSLYTIISVLFQR*PSWIW*GENVYFI TSFTLAI*RQRQILFLEKRILALLFVMA**H*L ISIEGCTFPNSQVVFDIQS*IHSAFILVTYNYF YSSPKGVRLFTTTHSKVEINHRCR*TQKFNSCFL NGLLCPFCY*GGI*SISHKIGKGVEKAVTDNLN NTPEII*ESDHFKTHFLCNCIENCICFADICVF HICEWFHSLSCTFSRHFFEWMMFREVYCIFTFFL PYH*HKK*IKRWV*QGSSLLHTAVYVAVSCFST TATTTILSCKCCILLWWR*RFLEFCFKIKAKVS VLH*I*YAHSAFRGTAYNLRPPLSVFI*MART*V SVDFTIEMTKKQRRQH*NNIVSNC*GLAVSSFC PLGIRHGFILLH*PRDFTFKKY*NSKILL

Frame 1

VGAEGGRAPALPFLLPGSFAFLRRVCGGARLHR GDRAWQTPDRAEATQRARERLHRRAPA*PFRIL RAEKFHLLYAILESCLG*RSHSVYITSTVLAPNV LCVAAPLVESPGPCWGTRWQDVSVVRTSAA*LK IPGAGSPAL**QFSHGNQTVPGTVVRKARLGAR CQ*CFICHHPFS*PPVTAG*SI*SLFFGE*LLAT A*HKEKQA*SSG*FSGRPNPDVYDHLQLSEGRK ENSGKRPEI*SGSVGEYSEHSDVRQTERA*Q*S QKCEGQGYVYRA*NQEPGRFTR*I*LQMQNLAEQ RTRDQWCGKE*SETRTAVTQEDVFNA*Q*EKGS SSQNNRVAECH*TYPECPD***TSGVEAETAER LYWGAAQCLLGSAAELVHYSCGESAASSAAA*KV GGIGTEIHLRT*PYHKKQTSVMGPHLQSFPAAH SELVCGGKTALHANAPSEAAGLEDRGPVHCEVE TVGEIARAEL*FESQSLI**RCE*EKYSKRI*EV QHFGHAHKSDEHGGVHQWQSGG*ISAPAIERTE KCWHQNE*GSSHRY*RASLP*F*NPIVPAWFGN *PRDDLSARCGDLQRQPAPERLGLHPLVQHAGGG TQESVLLPDSTMCTMGSAFRSAELAVFFCHQKR SQCGPAEHVGREASWS*RQPRWSHSVDEVL*GK YK**KFSLLALD*KHPRTH*KTPAPSLE*WVHHG LHQQGARACPVEGPAAGDLPAAVQ*ELPGRGHH IHMGGAVPERRRT*LPCG*TLHEERTFCCYFP* HHSQLQSHGC*EYS*ESPEVSVSKY*QRPCLWKV LLQAKGSTRANGT*WP*RNWIYQD*VDFCV*SS PF*TSDHRQPAPHVS*GV*RGVSDSGLCRIRQY DEHSIEHEFFSSSLATVFLLICDSLLLLCSFTSC VSREMKERPANSLQPVDSK*IFL*LRNISYSEG HHASY*R*N*KAFSEEWVSQVKNIQIHPKYQDE NEGPLGKEKLSNI*QMLCIKSVPNCYRLLDKSVV I*GTA*RRNGKFLWENSYMFSLL*V*LAVFHWF TCEIVQSQVYIQLYQSSFKGSHHGSGRGKMCIL LHLSHWLFKDKDKFCFLRREY*LYCLLWLNDTS* YQ*KDVHFQIHKLCLISKAEYILLSSWSHTIIF TVLPRELGYSQPLIQKLKLTIDVDKLRNLIHVS *MGYFVLFVIRVVFSLLATKLGKE*KKQ*LTT*I IHQR*YENQIISKLISYVTALRTAYVSLIYVFF TFANGSILSPVLFPDTFLSG*CFVKYTVFLPFS FLITDTKSRLRDGFDKVLPFYILLSMWLYLVFPL LLPQLYYHANAVFFFGGDKDFLSFVLKLKLKYL YCIKYNMHTVLSVALHTI*GLLSQFLYRWREPK FQLILQLK*LKNKEDNIKTILFLIAEV*LSVLFA LWEFGMVSFYCTSQETLLLRSIKILKFY*

Frame 2

WGRKGAGRQRCLFSCRVVSLSCAESAEGLGCTG GIAPGRPQTEQRRPSALGRGCTAAPPPSPSGSC AQKSFICCMPSSRAV*VNVRTLCI*PRQSWHLT CCA*LLLWLNPQALVGAQGGRMSQWYELQQLDSK FLEQVHQLYDDSFPMEIRQYLAQWLEKQDWEHA ANDVSFATIRFHDLLSQLDDQYSRFSLENNFLL QHNIRKSKRNLQDNFQEDPIQMSMIIYSCLKEER KILENAQRFNQAQSGNIQSTVMLDKQKELDSKV RNVKDKVMCIEHEIKSLEDLQDEYDFKCKTLQN REHETNGVAKSDQKQEQLLLKKMYLMLDNKRKEV VHKIIELLNVTELTQNALINDELVEWKRRQQSA CIGGPPNACLDQLQNWFTIVAESLQQVRQQLKK LEELEQKYTYEHDPITKNKQVLWDRTFSLFQQLI QSSFVVERQPCMPTHPQRPLVLKTGVQFTVKLR LLVKLQELNYNLKVKVLFDKDVNERNTVKGFRK FNILGTHTKVMNMEESTNGSLAAEFRHLQLKEQK NAGTRTNEGPLIVTEELHSLSFETQLCQPGLVI DLETTSLPVVVISNVSQLPSGWASILWYNMLVA EPRNLSFFLTPPCARWAQLSEVLSWQFSSVTKRG LNVDQLNMLGEKLLGPNASPDGLIPWTRFCKEN INDKNFPFWLWIESILELIKKHLLPLWNDGCIM GFISKERERALLKDQQPGTFLLRFSESSREGAIT FTWVERSQNGGEPDFHAVEPYTKKELSAVTFPD IIRNYKVMAAENIPENPLKYLYPNIDKDHAFGK YYSRPKEAPEPMELDGPKGTGYIKTELISVSEVH PSRLQTTDNLLPMSPEEFDEVSRIVGSVEFDSM MNTV*SMNFFHLLWRQFSFSSVIPSCYSVPSHP VFLGK*KKGQQIRCNLLIASEFFSNSETSVTLKG IMHLTEGKIERHSLKSGFHK*KTSRYTQSIRTR MRVLWERRS*ATSSKCYA*SQCPTVIGCWINQW LFRELLDVGTVNFCGRILTCFLCFKCNWQFSIGL PVK*FKAKFIYNYISPLSKVAIMDLVGGKCVFY YIFHIGYLKTKTNSVS*EENISFTVCYGLMTLA NINRRMYISKFTSCV*YPKLNTFCFHLGHIQLFL QFSQGS*AIHNHSFKS*N*P*M*INSEI*FMFL KWATLSFLLLGWYLVY*PQNWERSRKSSN*QLE *YTRDNMRIRSFQNSFPM*LH*ELHMFR*YMCFS HLRMVPFSLLYFFQTLF*VDDVS*SILYFYLFP SLSLTQKVD*EMGLTRFFPFTYCCLCGCILFFH YCYHNYIIMQMLYSSLVEIKIS*VLF*N*S*SIC IALNIICTQCFPWHCIQSEASSLSFYIDGENLS FS*FYN*ND*KTKKTTLKQYCF*LLRFSCQFFL PFGNSAWFHFTALAKRLYF*EVLKF*NSI

Sequence STAT1.fAug05

STAT1.u.f.1 (forward primer)
STAT1.u.r.5 (reverse primer)
Single exon skipping

Frame 0

GGGGRGPGASAAFSPAG*FRFPAQSLRRGSAAP GGSRLADPRPSRGDPARSGEAAPPRPRLALPDPA RRKVSFAVCHPRELSRLTFALCVYNLDSLGT*R AVRSCSFG*IPRPLLGHKVAGIHQCTESSTNIH PFKW*KQTIMKIKYDVSVVRTSAA*LKIPGAGSP AL**QFSHGNQTVPGTVVRKARLGARCQ*CFIC HHPFS*PPVTAG*SI*SLFFGE*LLATA*HKEK QA*SS

Frame 1

VGAEGGRAPALPFLLPGSFAFLRRVCGGARLHR GDRAWQTPDRAEATQRARERLHRRAPA*PFRIL RAEKFHLLYAILESCLG*RSHSVYITSTVLAPNV LCVAAPLVESPGPCWGTRWQGYTSAQNPPPTSI PSSGENRQ**K*NMMSQWYELQQLDSKFLEQVH QLYDDSFPMEIRQYLAQWLEKQDWEHAANDVSFA TIRFHDLLSQLDDQYSRFSLENNFLLQHNIRKS KRNLQ

Frame 2

WGRKGAGRQRCLFSCRVVSLSCAESAEGLGCTG GIAPGRPQTEQRRPSALGRGCTAAPPPSPSGSC AQKSFICCMPSSRAV*VNVRTLCI*PRQSWHLT CCA*LLLWLNPQALVGAQGGRDTPVHRILHQHPS LQVVKTDNNENKI*CLSGTNFSSLTQNSWSRFT SFMMTVFPWKSDSTWHSG*KSKTGSTLPMMFHL PPSVFMTSCHSWMINIVAFLWRITSCYSIT*GKA SVIF