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Sequences

Sequence TLK1.eAug05

AGCCGAGATCATGCCACTGCACTCCAGCCTGGGAGACAGAGTGAGACTCCGTCTAAACAACAACAACAACAACAACAACAACAACAACAACAACAACAAC AAAGACACCCCCCCGCCATCCCATGCTTATTATATCATTCCTTTGAGATTCAAACTTCTGGGGAAGAGAGTTGATTAATTTTCCCCTTTGTTTCTCGATT ATAGGCCTCAACAAATAACGAAAGCTCTAATCACAGTTTTGGAAGCTTGGGATCTTTAAGTGACAAAGAATCAGAGACACCGGAGAAGAAACAATCGGAA TCATCCAGGGGAAGAAAGAGAAAAGCAGAAAACCAGAATGAAAGTAGTCAGGGAAAAAGTATTGGGGGACGTGGCCACAAAATTAGCGACTATTTTGAAT ACCAGGGTGGAAATGGCTCAAGTCCAGTAAGAGGCATACCTCCTGCAATCCGTTCTCCTCAAAATTCACATTCACATTCCACTCCTTCCTCATCTGTTCG ACCGAATAGCCCTTCTCCTACTGCATTAGCATTTGGGGACCACCCTATTGTACAACCAAAGCAATTATCCTTTAAAATTATTCAGACTGATCTCACAATG CTGAAATTAGCAGCATTAGAAAGTAATAAAATCCAGGACCTGGAAAAGAAGGAGGGACGTATAGATGATTTGCTCAGGGCTAACTGTGATCTCAGACGGC AAATAGATGAACAACAAAAATTACTTGAAAAATACAAAGAACGATTAA

TLK1.u.f.12 (forward primer)
TLK1.u.r.11 (reverse primer)
Single exon skipping

Frame 0

SRDHATALQPGRQSETPSKQQQQQQQQQQQQQQ QRHPPAIPCLLYHSFEIQTSGEES*LIFPFVSRL *ASTNNESSNHSFGSLGSLSDKESETPEKKQSE SSRGRKRKAENQNESSQGKSIGGRGHKISDYFE YQGGNGSSPVRGIPPAIRSPQNSHSHSTPSSSVR PNSPSPTALAFGDHPIVQPKQLSFKIIQTDLTM LKLAALESNKIQDLEKKEGRIDDLLRANCDLRR QIDEQQKLLEKYKERL

Frame 1

AEIMPLHSSLGDRVRLRLNNNNNNNNNNNNNNN KDTPPPSHAYYIIPLRFKLLGKRVD*FSPLFLD YRPQQITKALITVLEAWDL*VTKNQRHRRRNNRN HPGEEREKQKTRMKVVREKVLGDVATKLATILN TRVEMAQVQ*EAYLLQSVLLKIHIHIPLLPHLF DRIALLLLH*HLGTTLLYNQSNYPLKLFRLISQC *N*QH*KVIKSRTWKRRRDV*MICSGLTVISDG K*MNNKNYLKNTKND*

Frame 2

PRSCHCTPAWETE*DSV*TTTTTTTTTTTTTTT KTPPRHPMLIISFL*DSNFWGRELINFPLCFSI IGLNK*RKL*SQFWKLGIFK*QRIRDTGEETIG IIQGKKEKSRKPE*K*SGKKYWGTWPQN*RLF*I PGWKWLKSSKRHTSCNPFSSKFTFTFHSFLICS TE*PFSYCISIWGPPYCTTKAIIL*NYSD*SHN AEISSIRK**NPGPGKEGGTYR*FAQG*L*SQTA NR*TTKIT*KIQRTI

Sequence TLK1.dAug05

TLK1.u.f.12 (forward primer)
TLK1.u.r.11 (reverse primer)
Single exon skipping

Frame 0

Frame 1

Frame 2

Sequence TLK1.cAug05

ACTGTTTATTTGTGAGGGTTAAAGTAAATACTTGTAGTGGGTGGAATGGTGGTCCTCCAAAAGCTATGTCCACCAAAAGCCTGCGAATATGACCCTATTT GGAAAAAGGGTCTTTGCAGATATAATCAAGTTAAGGGTTTTGAGATGAGATCATACTGGGTTAGGGCGGGCTCTCAATGCAATGATCAGTGTCCTAGAAG AGAAGAGAGGGAGAAGGCACACTGAGGAATGCCAAGGACTGCCAGCAGCACCAGAAGCTAGGAGAGAGGCACAGAACAGACTCCTAGCTTCTCCTAGGCT CCCTCTGAGCCTCCAGGAAGAACCAATCCCGGTGACATCTTGATTTTGGACTTCTGGCATCAGAACTGTGCAATGGATGAGCTTCATAGTCTGGATCCAA GAAGGCAAGAGTTATTGGAAGCTAGATTTACTGGAGTTGCAAGTGGGAGCACTGGAAGTACGGGCAGTTGCAGTGTTGGAGCTAAAGCCTCAACAAATAA CGAAAGCTCTAATCACAGTTTTGGAAGCTTGGGATCTTTAAGTGACAAAGAATCAGAGACACCGGAGAAGAAACAATCGGAATCATCCAGGGGAAGAAAG AGAAAAGCAGAAAACCAGAATGAAAGTAGTCAGGGAAAAAGTATTGGGGGACGTGGCCACAAAATTAGCGACTATTTTGAATACCAGGGTGGAAATGGCT CAAGTCCAGTAAGAGGCATACCTCCTGCAATCCGTTCTCCTCAAAATTCACATTCACATTCCACTCCTTCCTCATCTGTTCGACCGAATAGCCCTTCTCC TACTGCATTAGCATTTGGGGACCACCCTATTGTACAACCAAAGCAATTATCCTTTAAAATTATTCAGACTGATCTCACAATGCTGAAATTAGCAGCATTA GAAAGTAATAAAATCCAGGACCTGGAAAAGAAGGAGGGACGTATAGATGATTTGCTCAGGGCTAACTGTGATCTCAGACGGCAAATAGATGAACAACAAA AATTACTTGAAAAATACAAAGAACGATTAAATAAGTGCATATCAATGAGCAAGAAACTTCTTATTGAAAAGAGTACACAAGAAAAGCTGTCAAGCAGAGA GAAGAGTATGCAAGATCGATTACGCCTCGGGCACTTTACAACAGTTAGACATGGCGCTTCATTTACTGAACAATGGACAGATGGTTTTGCATTTCAGAAT CTTGTGAAGCAACAAGAATGGGTGAATCAGCAAAGGGAAGATATTGAAAGGCAAAGGAAACTTCTAGCCAAACGCAAACCTCCCACAGCTAATAATTCTC AGGCACCCTCTACCAATTCTGAACCAAAACAAAGGAAAAACAAAGCAGTCAATGGAGCAGAGAATGATCCCTTTGTTAGACCAAATTTACCACAACTGTT GACTTTGGCAGAATATCATGAACAGGAAGAAATTTTCAAACTTAGACTAGGACATCTCAAAAAGGAAGAGGCAGAAATCCAGGCAGAACTTGAACGTTTG GAAAGAGTCAGAAATCTTCACATACGTGAGCTGAAAAGAATAAACAATGAAGATAATTCACAGTTCAAAGATCACCCAACATTAAATGAAAGATATTTAT TACTTCATCTGCTTGGTAGAGGTGGCTTTAGTGAAGTGTATAAGGCTTTTGACCTTTATGAACAAAGATATGCTGCTGTGAAGATACATCAGCTTAATAA AAGCTGGAGAGATGAGAAGAAAGAAAACTACCACAAACATGCCTGCAGAGAGTATAGAATACACAAAGAACTGGATCACCCCAGAATAGTTAAACTCTAT GATTATTTCTCCTTGGATACAGATACGTTTTGTACAGTGTTAGAATACTGTGAAGGCAATGACTTGGATTTCTATCTGAAGCAACACAAGTTAATGTCAG AGAAAGAAGCTCGGTCTATTGTAATGCAGATTGTAAATGCACTAAGATATCTCAATGAGATCAAACCCCCTATTATACATTATGATCTTAAGCCAGGAAA CATCCTACTGGTAGATGGAACAGCATGTGGTGAAATCAAAATCACTGATTTTGGTCTGTCCAAGATTATGGATGATGATAGCTATGGTGTAGATGGAATG GATCTAACTTCCCAGGGGGCAGGCACTTACTGGTATTTACCTCCTGAGTGTTTTGTAGTTGGAAAAGAGCCACCAAAGATTTCCAACAAGGTTGATGTAT GGTCGGTTGGAGTCATCTTCTTTCAGTGTCTTTATGGTAGAAAGCCATTTGGTCACAATCAATCTCAACAAGACATTCTTCAAGAAAATACAATATTAAA AGCCACAGAAGTCCAGTTCCCTGTAAAACCGGTTGTAAGCAGTGAAGCCAAGGCATTTATAAGACGCTGTTTGGCATATCGAAAAGAAGATCGATTTGAT GTGCACCAGCTGGCAAATGACCCATACCTTCTCCCACACATGAGAAGATCAAATTCTTCAGGAAACCTACACATGGCTGGGCTGACAGCATCCCCTACAC CCCCTTCTTCAAGCATAATTACTTACTGACTTTCCTCCAAGATTGGCATGATATCTTTGAATTTGCTTCCAGATGCACACTTAAGTTTGAGAGCATTTGA GTGTTTGTTTTCTTTTTCTTTTTTTTTTTTTTTTTTTTTTACACAAGACGTGGTTAAGAACTGTTTGTGAACTGAAGTTCCTCATAGTGTCATTTGTATG AGAGAGGATCATGGACAATGAATAAATGCACACTTCTGACTATAATTTTGAGCAGTGAAGGAAGGATGACTGCCTGTTACACAGAAACAGGAATACCATG TTAAGATAGAGGAAAAAAATTTTGTTTATTTGGGGGAACACTGTAAATAACGTTTATAATACTTAAATACATTTGGTTGTTACTAGAGAGTTCTAATATG AAGGGTAGTTTTTCATTATTTAAAAACACATGGAAAAATATGAGACATATTTCTAACATACGAACTATAGTGCCTGGATACATTCTTCAGCATTTGGCAG TTAATCTGCTGAAACCAAAGTAAGAACTGAATGACAGTGACAGTTGTGTTTTATAGTAATAGAATGTGAGAGTTAGAATCTTTGGACAAAGTTAGGCTTT GTCATTTGCCTATTCAGACTTTTACCAAGTTGTACCTCGAGATCACATGTCCGTTTTCTTTCACTGGTTATATTAAAGGGAGAGCTATTATTTCTAAATA CTTTACACCTTTGACAAAATGAGCTGCTTGTGTTGAAATCAGTTGAATTCACTAAATTATAATATTGCCAGTGTTTTCAACACGTTAGAATTGATAACAG GTATTTTTCTTTTGTTACCAGGCCTTGTTCATCAAAACAGGTGACAGTGAGTAACCAAATGCAGACCAGTTCTATGCAGGATAATGATAAATTCCCTTCT AGCTCTATTGTAAATTGTTGTACAGATGTCATATTTCAATTACTAAGTTTCAGCAGCTTATTCTTGTACAAATGTTTAAAATATGGCTTTTCTAATTGGA TATTGTATTTTTTTTAAGTCTTCTTGAAGGCACTCTAATTGCTGCTAAATGATGTTGCTTCTTTGCTAAAAAATTAAATGACCTAGAAGGTGCAAGTTGA CTGTACATCATAGATATTAAAAAGCAGACAGTCATTAACAATCAAGATGTAAAAGTGATCCATGTTGGACATAATTGAGTTTTTAAATCAGTTTATTGGG TTCTAGGGCTGTAGAACACATAGATACTAGATTTTTAATTTGTTCATGGTTATTCTACATTTCTAGAAAGTTCTTATCAGCAAGATGGTCCCATAAGCAA ATTTCTTGTGGTTTGTACAGATTTGTTAAAATGTGAACGTTTTCTAACTGCCTCGTAGGGTAGAAACCAATATTTTTCAGGATGCTGTATTTCACTCCTG TAAGGACTTTTTCTTTATTGTCACATTCATAATGCTGATACTTACATGTAAGTTGTTCATGTTGCAGAAAAAGGTTATACGTACCACGGATTCTCTTTAA TATTGTACAGTTAAACTGTATTTCTGATGTTGCAAGCCATTTTGTTTTTTCATTGTACATAGACACATTGTCTCTTTAAAATGCTGCTGAAATTGTAGAG AGTATAGCCAAAACAGTAATAAACAATGACAGTTAAAATGTAAAGACTATGAAAATTACATTGGAAGGGAGCTTTCAAGATGGTAGGATATTGACTAACT GAGCTCCTTCACTTAAAAATCAGCTGAATAAATTCCTTAAGTATACTTCAAGACAAGTGTAAATTGTTTCAAGCTGTTAAAGTTAAACCTAATAACCATT ATCAAGGCAACTCCTGGGTAAGTAACACTTAGAACCATCAAGAGTGGTTTAAGGGGCTGGCTATACCCATTCCTCTGTAGAGCACAGAGAAATCCTATTA AAAAAAAAATACTACATGGTAATGAAATCTTAGCATTGATTGAGTGCTTTACGTGCTGCCTGCAAGTTTTCAGTTTTGTTGTTAGAGTTTTCGTCTTTAT GAATGATAAAGTCCTATGTTCATAAAGTAACATTTAAATTCCTGAACTAAAAGGAAATGCCTGTTGTGCTTAAAATTTTGGGGAGATACGAAGTGAATGT GTAAATCTAACTTTGAGGTTCAGTAAGTTAAGTAGTATAGACCTAGAGATCACTGCTTTACCATTTTACTTTCAAATGAATGAGAGCGACTGCTAGTGGC AGAACGCAGAAAAGGGGAAAAACAAGCCATGAAATGAAGTTGCTTTCCTGACCTGGGTTTAGGTCATCATTTTAAAATCAAAGATGTGTAGGAGATGAAT CAACTTATAGACCCATATTTTCCTTCTAAAAGTTTCCCAAAGAAATGTTATAGATTTTGAATAACATGCCAGTGACCCAGCAGGTCCAAGATGGCTAACC AAGATGGCTAATTTAGCCACATTGAATAACTTACTTTTACCCTCACATGGAAAGTTGGTATCTATTGCTAAATGATTTCAAGGTCACTCTGACAGTCTTT GACACTGTTTTTTTTAGGGTTTTATTGTTTTCTGGTCTCCTGGTGTCTGTCCTCAGAATTAAAGCATGTCCTGCATCCCTAAGATGATAGCCAAGGCCTC TCTTTTATTAAGAGTCAGCACTGATGTAGGGTAAAATCAACAGTTTTTGCATATGATTTTAAAATCTTTCTTTGATTTGAATTTGCTTTAGTGTGTGCAT GTTGAGAGGAAGTAAGTGTAGAAACCACCCTAATTTGTAAGTTGGGAGTTGGAGATACTTGGTGGTTTTTCAAAAATAAAGACTCAATCTTTTTTTTTTT TTTAAACACTCACTACCATCTCCTTCTAAAACAACTCTGTCAGTTTAAATGGCATATAAAATGTGAACAAACACCTCATTACATGAAGTCAAAATGGTGA AGAGCTTCAGTGAAACATTTAAAGAGTATATTGTGTGTTGTTTTGCTGTTCATCATTACTTATTAAATGCAGTAATGTTGATTGCC

TLK1.u.f.12 (forward primer)
TLK1.u.r.11 (reverse primer)
Single exon skipping

Frame 0

TVYL*GLK*ILVVGGMVVLQKLCPPKACEYDPI WKKGLCRYNQVKGFEMRSYWVRAGSQCNDQCPRR EEREKAH*GMPRTASSTRS*ERGTEQTPSFS*A PSEPPGRTNPGDILILDFWHQNCAMDELHSLDP RRQELLEARFTGVASGSTGSTGSCSVGAKASTNN ESSNHSFGSLGSLSDKESETPEKKQSESSRGRK RKAENQNESSQGKSIGGRGHKISDYFEYQGGNG SSPVRGIPPAIRSPQNSHSHSTPSSSVRPNSPSP TALAFGDHPIVQPKQLSFKIIQTDLTMLKLAAL ESNKIQDLEKKEGRIDDLLRANCDLRRQIDEQQ KLLEKYKERLNKCISMSKKLLIEKSTQEKLSSRE KSMQDRLRLGHFTTVRHGASFTEQWTDGFAFQN LVKQQEWVNQQREDIERQRKLLAKRKPPTANNS QAPSTNSEPKQRKNKAVNGAENDPFVRPNLPQLL TLAEYHEQEEIFKLRLGHLKKEEAEIQAELERL ERVRNLHIRELKRINNEDNSQFKDHPTLNERYL LLHLLGRGGFSEVYKAFDLYEQRYAAVKIHQLNK SWRDEKKENYHKHACREYRIHKELDHPRIVKLY DYFSLDTDTFCTVLEYCEGNDLDFYLKQHKLMS EKEARSIVMQIVNALRYLNEIKPPIIHYDLKPGN ILLVDGTACGEIKITDFGLSKIMDDDSYGVDGM DLTSQGAGTYWYLPPECFVVGKEPPKISNKVDV WSVGVIFFQCLYGRKPFGHNQSQQDILQENTILK ATEVQFPVKPVVSSEAKAFIRRCLAYRKEDRFD VHQLANDPYLLPHMRRSNSSGNLHMAGLTASPT PPSSSIITY*LSSKIGMISLNLLPDAHLSLRAFE CLFSFSFFFFFFFTQDVVKNCL*TEVPHSVICM REDHGQ*INAHF*L*F*AVKEG*LPVTQKQEYH VKIEEKNFVYLGEHCK*RL*YLNTFGCY*RVLI* RVVFHYLKTHGKI*DIFLTYEL*CLDTFFSIWQ LIC*NQSKN*MTVTVVFYSNRM*ELESLDKVRL CHLPIQTFTKLYLEITCPFSFTGYIKGRAIISKY FTPLTK*AACVEIS*IH*IIILPVFSTR*N**Q VFFFCYQALFIKTGDSE*PNADQFYAG***IPF *LYCKLLYRCHISITKFQQLILVQMFKIWLF*LD IVFFLSLLEGTLIAAK*CCFFAKKLNDLEGAS* LYIIDIKKQTVINNQDVKVIHVGHN*VFKSVYW VLGL*NT*ILDF*FVHGYSTFLESSYQQDGPISK FLVVCTDLLKCERFLTAS*GRNQYFSGCCISLL *GLFLYCHIHNADTYM*VVHVAEKGYTYHGFSL ILYS*TVFLMLQAILFFHCT*THCLFKMLLKL*R V*PKQ**TMTVKM*RL*KLHWKGAFKMVGY*LT ELLHLKIS*INSLSILQDKCKLFQAVKVKPNNH YQGNSWVSNT*NHQEWFKGLAIPIPL*STEKSY* KKNTTW**NLSID*VLYVLPASFQFCC*SFRLY E**SPMFIK*HLNS*TKRKCLLCLKFWGDTK*M CKSNFEVQ*VK*YRPRDHCFTILLSNE*ERLLVA ERRKGEKQAMK*SCFPDLGLGHHFKIKDV*EMN QLIDPYFPSKSFPKKCYRF*ITCQ*PSRSKMAN QDG*FSHIE*LTFTLTWKVGIYC*MISRSL*QSL TLFFLGFYCFLVSWCLSSELKHVLHP*DDSQGL SFIKSQH*CRVKSTVFAYDFKIFL*FEFALVCA C*EEVSVETTLICKLGVGDTWWFFKNKDSIFFFF LNTHYHLLLKQLCQFKWHIKCEQTPHYMKSKW* RASVKHLKSILCVVLLFIITY*MQ*C*L

Frame 1

LFICEG*SKYL*WVEWWSSKSYVHQKPANMTLF GKRVFADIIKLRVLR*DHTGLGRALNAMISVLE EKRGRRHTEECQGLPAAPEARREAQNRLLASPRL PLSLQEEPIPVTS*FWTSGIRTVQWMSFIVWIQ EGKSYWKLDLLELQVGALEVRAVAVLELKPQQI TKALITVLEAWDL*VTKNQRHRRRNNRNHPGEER EKQKTRMKVVREKVLGDVATKLATILNTRVEMA QVQ*EAYLLQSVLLKIHIHIPLLPHLFDRIALL LLH*HLGTTLLYNQSNYPLKLFRLISQC*N*QH* KVIKSRTWKRRRDV*MICSGLTVISDGK*MNNK NYLKNTKND*ISAYQ*ARNFLLKRVHKKSCQAE RRVCKIDYASGTLQQLDMALHLLNNGQMVLHFRI L*SNKNG*ISKGKILKGKGNF*PNANLPQLIIL RHPLPILNQNKGKTKQSMEQRMIPLLDQIYHNC *LWQNIMNRKKFSNLD*DISKRKRQKSRQNLNVW KESEIFTYVS*KE*TMKIIHSSKITQH*MKDIY YFICLVEVALVKCIRLLTFMNKDMLL*RYISLI KAGEMRRKKTTTNMPAESIEYTKNWITPE*LNSM IISPWIQIRFVQC*NTVKAMTWISI*SNTS*CQ RKKLGLL*CRL*MH*DISMRSNPLLYIMILSQE TSYW*MEQHVVKSKSLILVCPRLWMMIAMV*MEW I*LPRGQALTGIYLLSVL*LEKSHQRFPTRLMY GRLESSSFSVFMVESHLVTINLNKTFFKKIQY* KPQKSSSL*NRL*AVKPRHL*DAVWHIEKKIDLM CTSWQMTHTFSHT*EDQILQETYTWLG*QHPLH PLLQA*LLTDFPPRLA*YL*ICFQMHT*V*EHL SVCFLFLFFFFFFLHKTWLRTVCELKFLIVSFV* ERIMDNE*MHTSDYNFEQ*RKDDCLLHRNRNTM LR*RKKILFIWGNTVNNVYNT*IHLVVTREF*Y EG*FFII*KHMEKYETYF*HTNYSAWIHSSAFGS *SAETKVRTE*Q*QLCFIVIECES*NLWTKLGF VICLFRLLPSCTSRSHVRFLSLVILKGELLFLN TLHL*QNELLVLKSVEFTKL*YCQCFQHVRIDNR YFSFVTRPCSSKQVTVSNQMQTSSMQDNDKFPS SSIVNCCTDVIFQLLSFSSLFLYKCLKYGFSNW ILYFF*VFLKAL*LLLNDVASLLKN*MT*KVQVD CTS*ILKSRQSLTIKM*K*SMLDIIEFLNQFIG F*GCRTHRY*IFNLFMVILHF*KVLISKMVP*A NFLWFVQIC*NVNVF*LPRRVETNIFQDAVFHSC KDFFFIVTFIMLILTCKLFMLQKKVIRTTDSL* YCTVKLYF*CCKPFCFFIVHRHIVSLKCC*NCR EYSQNSNKQ*QLKCKDYENYIGRELSRW*DID*L SSFT*KSAE*IP*VYFKTSVNCFKLLKLNLITI IKATPG*VTLRTIKSGLRGWLYPFLCRAQRNPI KKKILHGNEILALIECFTCCLQVFSFVVRVFVFM NDKVLCS*SNI*IPELKGNACCA*NFGEIRSEC VNLTLRFSKLSSIDLEITALPFYFQMNESDC*W QNAEKGKNKP*NEVAFLTWV*VIILKSKMCRR*I NL*THIFLLKVSQRNVIDFE*HASDPAGPRWLT KMANLATLNNLLLPSHGKLVSIAK*FQGHSDSL *HCFF*GFIVFWSPGVCPQN*SMSCIPKMIAKAS LLLRVSTDVG*NQQFLHMILKSFFDLNLL*CVH VERK*V*KPP*FVSWELEILGGFSKIKTQSFFF F*TLTTISF*NNSVSLNGI*NVNKHLIT*SQNGE ELQ*NI*RVYCVLFCCSSLLIKCSNVDC

Frame 2

CLFVRVKVNTCSGWNGGPPKAMSTKSLRI*PYL EKGSLQI*SS*GF*DEIILG*GGLSMQ*SVS*K RREGEGTLRNAKDCQQHQKLGERHRTDS*LLLG SL*ASRKNQSR*HLDFGLLASELCNG*AS*SGSK KARVIGS*IYWSCKWEHWKYGQLQCWS*SLNK* RKL*SQFWKLGIFK*QRIRDTGEETIGIIQGKK EKSRKPE*K*SGKKYWGTWPQN*RLF*IPGWKWL KSSKRHTSCNPFSSKFTFTFHSFLICSTE*PFS YCISIWGPPYCTTKAIIL*NYSD*SHNAEISSI RK**NPGPGKEGGTYR*FAQG*L*SQTANR*TTK IT*KIQRTIK*VHINEQETSY*KEYTRKAVKQR EEYARSITPRALYNS*TWRFIY*TMDRWFCISE SCEATRMGESAKGRY*KAKETSSQTQTSHS**FS GTLYQF*TKTKEKQSSQWSRE*SLC*TKFTTTV DFGRIS*TGRNFQT*TRTSQKGRGRNPGRT*TF GKSQKSSHT*AEKNKQ*R*FTVQRSPNIK*KIFI TSSAW*RWL**SV*GF*PL*TKICCCEDTSA** KLER*EERKLPQTCLQRV*NTQRTGSPQNS*TL *LFLLGYRYVLYSVRIL*RQ*LGFLSEATQVNVR ERSSVYCNADCKCTKISQ*DQTPYYTL*S*ARK HPTGRWNSMW*NQNH*FWSVQDYG***LWCRWN GSNFPGGRHLLVFTS*VFCSWKRATKDFQQG*CM VGWSHLLSVSLW*KAIWSQSISTRHSSRKYNIK SHRSPVPCKTGCKQ*SQGIYKTLFGISKRRSI* CAPAGK*PIPSPTHEKIKFFRKPTHGWADSIPYT PFFKHNYLLTFLQDWHDIFEFASRCTLKFESI* VFVFFFFFFFFFFYTRRG*ELFVN*SSS*CHLY ERGSWTMNKCTLLTIILSSEGRMTACYTETGIPC *DRGKKFCLFGGTL*ITFIILKYIWLLLESSNM KGSFSLFKNTWKNMRHISNIRTIVPGYILQHLA VNLLKPK*ELNDSDSCVL***NVRVRIFGQS*AL SFAYSDFYQVVPRDHMSVFFHWLY*RESYYF*I LYTFDKMSCLC*NQLNSLNYNIASVFNTLELIT GIFLLLPGLVHQNR*Q*VTKCRPVLCRIMINSLL ALL*IVVQMSYFNY*VSAAYSCTNV*NMAFLIG YCIFFKSS*RHSNCC*MMLLLC*KIK*PRRCKL TVHHRY*KADSH*QSRCKSDPCWT*LSF*ISLLG SRAVEHIDTRFLICSWLFYISRKFLSARWSHKQ ISCGLYRFVKM*TFSNCLVG*KPIFFRMLYFTP VRTFSLLSHS*C*YLHVSCSCCRKRLYVPRILFN IVQLNCISDVASHFVFSLYIDTLSL*NAAEIVE SIAKTVINNDS*NVKTMKITLEGSFQDGRILTN *APSLKNQLNKFLKYTSRQV*IVSSC*S*T**PL SRQLLGK*HLEPSRVV*GAGYTHSSVEHREILL KKKYYMVMKS*H*LSALRAACKFSVLLLEFSSL *MIKSYVHKVTFKFLN*KEMPVVLKILGRYEVNV *I*L*GSVS*VV*T*RSLLYHFTFK*MRATASG RTQKRGKTSHEMKLLS*PGFRSSF*NQRCVGDE STYRPIFSF*KFPKEML*ILNNMPVTQQVQDG*P RWLI*PH*ITYFYPHMESWYLLLNDFKVTLTVF DTVFFRVLLFSGLLVSVLRIKACPASLR**PRP LFY*ESALM*GKINSFCI*F*NLSLI*ICFSVCM LRGSKCRNHPNL*VGSWRYLVVFQK*RLNLFFF FKHSLPSPSKTTLSV*MAYKM*TNTSLHEVKMV KSFSETFKEYIVCCFAVHHYLLNAVMLIA

Sequence TLK1.bAug05

GCGTGACGCCGCCGCCGCCTGTAACTGATGCAGAGGTTACCCCACCGCCTCAGCCGCCACCCACAGCCCGCCGCCGCCGCCTCCCGGGCGACGCCGGGGG CCGACGCACTGCAGAGGTGCCGGCAGGTTCCGCCCGCGGAAGTGTCCTCGGCCGGCGCAGCCCGCGCTCCGCCCGCGCTCCGACCCCGGCCCGGCTGGCA CCCCCTCGACGCCTCCGCCGCCGCGCTCCTTTCCTCCTCCTCTCCTCTGGCGCGCCCGCTCGCTCCCTCGCTTCTCCCCCTTTCCCTCCTCCCTGTCCCC TCTCCTTCCCTCACCTCTTTCCTCCCCACCTCCCCTCTTCTCGGTTTCCTCCCCCATCCCCTTGACTCTCCCCTCCCAGCCCTCGCTCTCTCGCTCGCCC TCAGCGCGGCCCCCGCCATGACGGAGGCGGGTGCCGGTGCCGTTGCCGCCGCTGCCGTCGCAGGGGGGGAGTCGGGTTCCCAGAAAGTAGCTTGATGAGT GTCCAAAGTAGCAGTGGAAGTTTGGAGGGGCCGCCATCTTGGTCCCAGCTCTCCACGTCTCCAACCCCGGGCTCGGCGGCGGCGGCCAGGTCCCTGCTGA ATCACACGCCGCCATCCGGGAGGCCCAGGGAAGGTGCAATGGATGAGCTTCATAGTCTGGATCCAAGAAGGCAAGAGTTATTGGAAGCTAGATTTACTGG AGTTGCAAGTGGGAGCACTGGAAGTACGGGCAGTTGCAGTGTTGGAGCTAAAGCCTCAACAAATAACGAAAGCTCTAATCACAGTTTTGGAAGCTTGGGA TCTTTAAGTGACAAAGAATCAGAGACACCGGAGAAGAAACAATCGGAATCATCCAGGGGAAGAAAGAGAAAAGCAGAAAACCAGAATGAAAGTAGTCAGG GAAAAAGTATTGGGGGACGTGGCCACAAAATTAGCGACTATTTTGAATACCAGGGTGGAAATGGCTCAAGTCCAGTAAGAGGCATACCTCCTGCAATCCG TTCTCCTCAAAATTCACATTCACATTCCACTCCTTCCTCATCTGTTCGACCGAATAGCCCTTCTCCTACTGCATTAGCATTTGGGGACCACCCTATTGTA CAACCAAAGCAATTATCCTTTAAAATTATTCAGACTGATCTCACAATGCTGAAATTAGCAGCATTAGAAAGTAATAAAATCCAGGACCTGGAAAAGAAGG AGGGACGTATAGATGATTTGCTCAGGGCTAACTGTGATCTCAGACGGCAAATAGATGAACAACAAAAATTACTTGAAAAATACAAAGAACGATTAAATAA GTGCATATCAATGAGCAAGAAACTTCTTATTGAAAAGAGTACACAAGAAAAGCTGTCAAGCAGAGAGAAGAGTATGCAAGATCGATTACGCCTCGGGCAC TTTACAACAGTTAGACATGGCGCTTCATTTACTGAACAATGGACAGATGGTTTTGCATTTCAGAATCTTGTGAAGCAACAAGAATGGGTGAATCAGCAAA GGGAAGATATTGAAAGGCAAAGGAAACTTCTAGCCAAACGCAAACCTCCCACAGCTAATAATTCTCAGGCACCCTCTACCAATTCTGAACCAAAACAAAG GAAAAACAAAGCAGTCAATGGAGCAGAGAATGATCCCTTTGTTAGACCAAATTTACCACAACTGTTGACTTTGGCAGAATATCATGAACAGGAAGAAATT TTCAAACTTAGACTAGGACATCTCAAAAAGGAAGAGGCAGAAATCCAGGCAGAACTTGAACGTTTGGAAAGAGTCAGAAATCTTCACATACGTGAGCTGA AAAGAATAAACAATGAAGATAATTCACAGTTCAAAGATCACCCAACATTAAATGAAAGATATTTATTACTTCATCTGCTTGGTAGAGGTGGCTTTAGTGA AGTGTATAAGGCTTTTGACCTTTATGAACAAAGATATGCTGCTGTGAAGATACATCAGCTTAATAAAAGCTGGAGAGATGAGAAGAAAGAAAACTACCAC AAACATGCCTGCAGAGAGTATAGAATACACAAAGAACTGGATCACCCCAGAATAGTTAAACTCTATGATTATTTCTCCTTGGATACAGATACGTTTTGTA CAGTGTTAGAATACTGTGAAGGCAATGACTTGGATTTCTATCTGAAGCAACACAAGTTAATGTCAGAGAAAGAAGCTCGGTCTATTGTAATGCAGATTGT AAATGCACTAAGATATCTCAATGAGATCAAACCCCCTATTATACATTATGATCTTAAGCCAGGAAACATCCTACTGGTAGATGGAACAGCATGTGGTGAA ATCAAAATCACTGATTTTGGTCTGTCCAAGATTATGGATGATGATAGCTATGGTGTAGATGGAATGGATCTAACTTCCCAGGGGGCAGGCACTTACTGGT ATTTACCTCCTGAGTGTTTTGTAGTTGGAAAAGAGCCACCAAAGATTTCCAACAAGGTTGATGTATGGTCGGTTGGAGTCATCTTCTTTCAGTGTCTTTA TGGTAGAAAGCCATTTGGTCACAATCAATCTCAACAAGACATTCTTCAAGAAAATACAATATTAAAAGCCACAGAAGTCCAGTTCCCTGTAAAACCGGTT GTAAGCAGTGAAGCCAAGGCATTTATAAGACGCTGTTTGGCATATCGAAAAGAAGATCGATTTGATGTGCACCAGCTGGCAAATGACCCATACCTTCTCC CACACATGAGAAGATCAAATTCTTCAGGAAACCTACACATGGCTGGGCTGACAGCATCCCCTACACCCCCTTCTTCAAGCATAATTACTTACTGACTTTC CTCCAAGATTGGCATGATATCTTTGAATTTGCTTCCAGATGCACACTTAAGTTTGAGAGCATTTGAGTGTTTGTTTTCTTTTTCTTTTTTTTTTTTTTTT TTTTTTACACAAGACGTGGTTAAGAACTGTTTGTGAACTGAAGTTCCTCATAGTGTCATTTGTATGAGAGAGGATCATGGACAATGAATAAATGCACACT TCTGACTATAATTTTGAGCAGTGAAGGAAGGATGACTGCCTGTTACACAGAAACAGGAATACCATGTTAAGATAGAGGAAAAAAATTTTGTTTATTTGGG GGAACACTGTAAATAACGTTTATAATACTTAAATACATTTGGTTGTTACTAGAGAGTTCTAATATGAAGGGTAGTTTTTCATTATTTAAAAACACATGGA AAAATATGAGACATATTTCTAACATACGAACTATAGTGCCTGGATACATTCTTCAGCATTTGGCAGTTAATCTGCTGAAACCAAAGTAAGAACTGAATGA CAGTGACAGTTGTGTTTTATAGTAATAGAATGTGAGAGTTAGAATCTTTGGACAAAGTTAGGCTTTGTCATTTGCCTATTCAGACTTTTACCAAGTTGTA CCTCGAGATCACATGTCCGTTTTCTTTCACTGGTTATATTAAAGGGAGAGCTATTATTTCTAAATACTTTACACCTTTGACAAAATGAGCTGCTTGTGTT GAAATCAGTTGAATTCACTAAATTATAATATTGCCAGTGTTTTCAACACGTTAGAATTGATAACAGGTATTTTTCTTTTGTTACCAGGCCTTGTTCATCA AAACAGGTGACAGTGAGTAACCAAATGCAGACCAGTTCTATGCAGGATAATGATAAATTCCCTTCTAGCTCTATTGTAAATTGTTGTACAGATGTCATAT TTCAATTACTAAGTTTCAGCAGCTTATTCTTGTACAAATGTTTAAAATATGGCTTTTCTAATTGGATATTGTATTTTTTTTAAGTCTTCTTGAAGGCACT CTAATTGCTGCTAAATGATGTTGCTTCTTTGCTAAAAAATTAAATGACCTAGAAGGTGCAAGTTGACTGTACATCATAGATATTAAAAAGCAGACAGTCA TTAACAATCAAGATGTAAAAGTGATCCATGTTGGACATAATTGAGTTTTTAAATCAGTTTATTGGGTTCTAGGGCTGTAGAACACATAGATACTAGATTT TTAATTTGTTCATGGTTATTCTACATTTCTAGAAAGTTCTTATCAGCAAGATGGTCCCATAAGCAAATTTCTTGTGGTTTGTACAGATTTGTTAAAATGT GAACGTTTTCTAACTGCCTCGTAGGGTAGAAACCAATATTTTTCAGGATGCTGTATTTCACTCCTGTAAGGACTTTTTCTTTATTGTCACATTCATAATG CTGATACTTACATGTAAGTTGTTCATGTTGCAGAAAAAGGTTATACGTACCACGGATTCTCTTTAATATTGTACAGTTAAACTGTATTTCTGATGTTGCA AGCCATTTTGTTTTTTCATTGTACATAGACACATTGTCTCTTTAAAATGCTGCTGAAATTGTAGAGAGTATAGCCAAAACAGTAATAAACAATGACAGTT AAAATGTAAAGACTATGAAAATTACATTGGAAGGGAGCTTTCAAGATGGTAGGATATTGACTAACTGAGCTCCTTCACTTAAAAATCAGCTGAATAAATT CCTTAAGTATACTTCAAGACAAGTGTAAATTGTTTCAAGCTGTTAAAGTTAAACCTAATAACCATTATCAAGGCAACTCCTGGGTAAGTAACACTTAGAA CCATCAAGAGTGGTTTAAGGGGCTGGCTATACCCATTCCTCTGTAGAGCACAGAGAAATCCTATTAAAAAAAAAATACTACATGGTAATGAAATCTTAGC ATTGATTGAGTGCTTTACGTGCTGCCTGCAAGTTTTCAGTTTTGTTGTTAGAGTTTTCGTCTTTATGAATGATAAAGTCCTATGTTCATAAAGTAACATT TAAATTCCTGAACTAAAAGGAAATGCCTGTTGTGCTTAAAATTTTGGGGAGATACGAAGTGAATGTGTAAATCTAACTTTGAGGTTCAGTAAGTTAAGTA GTATAGACCTAGAGATCACTGCTTTACCATTTTACTTTCAAATGAATGAGAGCGACTGCTAGTGGCAGAACGCAGAAAAGGGGAAAAACAAGCCATGAAA TGAAGTTGCTTTCCTGACCTGGGTTTAGGTCATCATTTTAAAATCAAAGATGTGTAGGAGATGAATCAACTTATAGACCCATATTTTCCTTCTAAAAGTT TCCCAAAGAAATGTTATAGATTTTGAATAACATGCCAGTGACCCAGCAGGTCCAAGATGGCTAACCAAGATGGCTAATTTAGCCACATTGAATAACTTAC TTTTACCCTCACATGGAAAGTTGGTATCTATTGCTAAATGATTTCAAGGTCACTCTGACAGTCTTTGACACTGTTTTTTTTAGGGTTTTATTGTTTTCTG GTCTCCTGGTGTCTGTCCTCAGAATTAAAGCATGTCCTGCATCCCTAAGATGATAGCCAAGGCCTCTCTTTTATTAAGAGTCAGCACTGATGTAGGGTAA AATCAACAGTTTTTGCATATGATTTTAAAATCTTTCTTTGATTTGAATTTGCTTTAGTGTGTGCATGTTGAGAGGAAGTAAGTGTAGAAACCACCCTAAT TTGTAAGTTGGGAGTTGGAGATACTTGGTGGTTTTTCAAAAATAAAGACTCAATCTTTTTTTTTTTTTTAAACACTCACTACCATCTCCTTCTAAAACAA CTCTGTCAGTTTAAATGGCATATAAAATGTGAACAAACACCTCATTACATGAAGTCAAAATGGTGAAGAGCTTCAGTGAAACATTTAAAGAGTATATTGT GTGTTGTTTTGCTGTTCATCATTACTTATTAAATGCAGTAATGTTGATTGCC

TLK1.u.f.12 (forward primer)
TLK1.u.r.11 (reverse primer)
Single exon skipping

Frame 0

A*RRRRL*LMQRLPHRLSRHPQPAAAASRATPG ADALQRCRQVPPAEVSSAGAARAPPALRPRPGWH PLDASAAALLSSSSPLARPLAPSLLPLSLLPVP SPSLTSFLPTSPLLGFLPHPLDSPLPALALSLA LSAAPAMTEAGAGAVAAAAVAGGESGSQKVA**V SKVAVEVWRGRHLGPSSPRLQPRARRRRPGPC* ITRRHPGGPGKVQWMSFIVWIQEGKSYWKLDLL ELQVGALEVRAVAVLELKPQQITKALITVLEAWD L*VTKNQRHRRRNNRNHPGEEREKQKTRMKVVR EKVLGDVATKLATILNTRVEMAQVQ*EAYLLQS VLLKIHIHIPLLPHLFDRIALLLLH*HLGTTLLY NQSNYPLKLFRLISQC*N*QH*KVIKSRTWKRR RDV*MICSGLTVISDGK*MNNKNYLKNTKND*I SAYQ*ARNFLLKRVHKKSCQAERRVCKIDYASGT LQQLDMALHLLNNGQMVLHFRIL*SNKNG*ISK GKILKGKGNF*PNANLPQLIILRHPLPILNQNK GKTKQSMEQRMIPLLDQIYHNC*LWQNIMNRKKF SNLD*DISKRKRQKSRQNLNVWKESEIFTYVS* KE*TMKIIHSSKITQH*MKDIYYFICLVEVALV KCIRLLTFMNKDMLL*RYISLIKAGEMRRKKTTT NMPAESIEYTKNWITPE*LNSMIISPWIQIRFV QC*NTVKAMTWISI*SNTS*CQRKKLGLL*CRL *MH*DISMRSNPLLYIMILSQETSYW*MEQHVVK SKSLILVCPRLWMMIAMV*MEWI*LPRGQALTG IYLLSVL*LEKSHQRFPTRLMYGRLESSSFSVF MVESHLVTINLNKTFFKKIQY*KPQKSSSL*NRL *AVKPRHL*DAVWHIEKKIDLMCTSWQMTHTFS HT*EDQILQETYTWLG*QHPLHPLLQA*LLTDF PPRLA*YL*ICFQMHT*V*EHLSVCFLFLFFFFF FLHKTWLRTVCELKFLIVSFV*ERIMDNE*MHT SDYNFEQ*RKDDCLLHRNRNTMLR*RKKILFIW GNTVNNVYNT*IHLVVTREF*YEG*FFII*KHME KYETYF*HTNYSAWIHSSAFGS*SAETKVRTE* Q*QLCFIVIECES*NLWTKLGFVICLFRLLPSC TSRSHVRFLSLVILKGELLFLNTLHL*QNELLVL KSVEFTKL*YCQCFQHVRIDNRYFSFVTRPCSS KQVTVSNQMQTSSMQDNDKFPSSSIVNCCTDVI FQLLSFSSLFLYKCLKYGFSNWILYFF*VFLKAL *LLLNDVASLLKN*MT*KVQVDCTS*ILKSRQS LTIKM*K*SMLDIIEFLNQFIGF*GCRTHRY*I FNLFMVILHF*KVLISKMVP*ANFLWFVQIC*NV NVF*LPRRVETNIFQDAVFHSCKDFFFIVTFIM LILTCKLFMLQKKVIRTTDSL*YCTVKLYF*CC KPFCFFIVHRHIVSLKCC*NCREYSQNSNKQ*QL KCKDYENYIGRELSRW*DID*LSSFT*KSAE*I P*VYFKTSVNCFKLLKLNLITIIKATPG*VTLR TIKSGLRGWLYPFLCRAQRNPIKKKILHGNEILA LIECFTCCLQVFSFVVRVFVFMNDKVLCS*SNI *IPELKGNACCA*NFGEIRSECVNLTLRFSKLS SIDLEITALPFYFQMNESDC*WQNAEKGKNKP*N EVAFLTWV*VIILKSKMCRR*INL*THIFLLKV SQRNVIDFE*HASDPAGPRWLTKMANLATLNNL LLPSHGKLVSIAK*FQGHSDSL*HCFF*GFIVFW SPGVCPQN*SMSCIPKMIAKASLLLRVSTDVG* NQQFLHMILKSFFDLNLL*CVHVERK*V*KPP* FVSWELEILGGFSKIKTQSFFFF*TLTTISF*NN SVSLNGI*NVNKHLIT*SQNGEELQ*NI*RVYC VLFCCSSLLIKCSNVDC

Frame 1

RDAAAACN*CRGYPTASAATHSPPPPPPGRRRG PTHCRGAGRFRPRKCPRPAQPALRPRSDPGPAG TPSTPPPPRSFPPPLLWRARSLPRFSPFPSSLSP LLPSPLSSPPPLFSVSSPIPLTLPSQPSLSRSP SARPPP*RRRVPVPLPPLPSQGGSRVPRK*LDE CPK*QWKFGGAAILVPALHVSNPGLGGGGQVPAE SHAAIREAQGRCNG*AS*SGSKKARVIGS*IYW SCKWEHWKYGQLQCWS*SLNK*RKL*SQFWKLG IFK*QRIRDTGEETIGIIQGKKEKSRKPE*K*SG KKYWGTWPQN*RLF*IPGWKWLKSSKRHTSCNP FSSKFTFTFHSFLICSTE*PFSYCISIWGPPYC TTKAIIL*NYSD*SHNAEISSIRK**NPGPGKEG GTYR*FAQG*L*SQTANR*TTKIT*KIQRTIK* VHINEQETSY*KEYTRKAVKQREEYARSITPRA LYNS*TWRFIY*TMDRWFCISESCEATRMGESAK GRY*KAKETSSQTQTSHS**FSGTLYQF*TKTK EKQSSQWSRE*SLC*TKFTTTVDFGRIS*TGRN FQT*TRTSQKGRGRNPGRT*TFGKSQKSSHT*AE KNKQ*R*FTVQRSPNIK*KIFITSSAW*RWL** SV*GF*PL*TKICCCEDTSA**KLER*EERKLP QTCLQRV*NTQRTGSPQNS*TL*LFLLGYRYVLY SVRIL*RQ*LGFLSEATQVNVRERSSVYCNADC KCTKISQ*DQTPYYTL*S*ARKHPTGRWNSMW* NQNH*FWSVQDYG***LWCRWNGSNFPGGRHLLV FTS*VFCSWKRATKDFQQG*CMVGWSHLLSVSL W*KAIWSQSISTRHSSRKYNIKSHRSPVPCKTG CKQ*SQGIYKTLFGISKRRSI*CAPAGK*PIPSP THEKIKFFRKPTHGWADSIPYTPFFKHNYLLTF LQDWHDIFEFASRCTLKFESI*VFVFFFFFFFF FFYTRRG*ELFVN*SSS*CHLYERGSWTMNKCTL LTIILSSEGRMTACYTETGIPC*DRGKKFCLFG GTL*ITFIILKYIWLLLESSNMKGSFSLFKNTW KNMRHISNIRTIVPGYILQHLAVNLLKPK*ELND SDSCVL***NVRVRIFGQS*ALSFAYSDFYQVV PRDHMSVFFHWLY*RESYYF*ILYTFDKMSCLC *NQLNSLNYNIASVFNTLELITGIFLLLPGLVHQ NR*Q*VTKCRPVLCRIMINSLLALL*IVVQMSY FNY*VSAAYSCTNV*NMAFLIGYCIFFKSS*RH SNCC*MMLLLC*KIK*PRRCKLTVHHRY*KADSH *QSRCKSDPCWT*LSF*ISLLGSRAVEHIDTRF LICSWLFYISRKFLSARWSHKQISCGLYRFVKM *TFSNCLVG*KPIFFRMLYFTPVRTFSLLSHS*C *YLHVSCSCCRKRLYVPRILFNIVQLNCISDVA SHFVFSLYIDTLSL*NAAEIVESIAKTVINNDS *NVKTMKITLEGSFQDGRILTN*APSLKNQLNKF LKYTSRQV*IVSSC*S*T**PLSRQLLGK*HLE PSRVV*GAGYTHSSVEHREILLKKKYYMVMKS* H*LSALRAACKFSVLLLEFSSL*MIKSYVHKVTF KFLN*KEMPVVLKILGRYEVNV*I*L*GSVS*V V*T*RSLLYHFTFK*MRATASGRTQKRGKTSHE MKLLS*PGFRSSF*NQRCVGDESTYRPIFSF*KF PKEML*ILNNMPVTQQVQDG*PRWLI*PH*ITY FYPHMESWYLLLNDFKVTLTVFDTVFFRVLLFS GLLVSVLRIKACPASLR**PRPLFY*ESALM*GK INSFCI*F*NLSLI*ICFSVCMLRGSKCRNHPN L*VGSWRYLVVFQK*RLNLFFFFKHSLPSPSKT TLSV*MAYKM*TNTSLHEVKMVKSFSETFKEYIV CCFAVHHYLLNAVMLIA

Frame 2

VTPPPPVTDAEVTPPPQPPPTARRRRLPGDAGG RRTAEVPAGSARGSVLGRRSPRSARAPTPARLA PPRRLRRRAPFLLLSSGAPARSLASPPFPPPCP LSFPHLFPPHLPSSRFPPPSP*LSPPSPRSLARP QRGPRHDGGGCRCRCRRCRRRGGVGFPESSLMS VQSSSGSLEGPPSWSQLSTSPTPGSAAAARSLL NHTPPSGRPREGAMDELHSLDPRRQELLEARFTG VASGSTGSTGSCSVGAKASTNNESSNHSFGSLG SLSDKESETPEKKQSESSRGRKRKAENQNESSQ GKSIGGRGHKISDYFEYQGGNGSSPVRGIPPAIR SPQNSHSHSTPSSSVRPNSPSPTALAFGDHPIV QPKQLSFKIIQTDLTMLKLAALESNKIQDLEKK EGRIDDLLRANCDLRRQIDEQQKLLEKYKERLNK CISMSKKLLIEKSTQEKLSSREKSMQDRLRLGH FTTVRHGASFTEQWTDGFAFQNLVKQQEWVNQQ REDIERQRKLLAKRKPPTANNSQAPSTNSEPKQR KNKAVNGAENDPFVRPNLPQLLTLAEYHEQEEI FKLRLGHLKKEEAEIQAELERLERVRNLHIREL KRINNEDNSQFKDHPTLNERYLLLHLLGRGGFSE VYKAFDLYEQRYAAVKIHQLNKSWRDEKKENYH KHACREYRIHKELDHPRIVKLYDYFSLDTDTFC TVLEYCEGNDLDFYLKQHKLMSEKEARSIVMQIV NALRYLNEIKPPIIHYDLKPGNILLVDGTACGE IKITDFGLSKIMDDDSYGVDGMDLTSQGAGTYW YLPPECFVVGKEPPKISNKVDVWSVGVIFFQCLY GRKPFGHNQSQQDILQENTILKATEVQFPVKPV VSSEAKAFIRRCLAYRKEDRFDVHQLANDPYLL PHMRRSNSSGNLHMAGLTASPTPPSSSIITY*LS SKIGMISLNLLPDAHLSLRAFECLFSFSFFFFF FFTQDVVKNCL*TEVPHSVICMREDHGQ*INAH F*L*F*AVKEG*LPVTQKQEYHVKIEEKNFVYLG EHCK*RL*YLNTFGCY*RVLI*RVVFHYLKTHG KI*DIFLTYEL*CLDTFFSIWQLIC*NQSKN*M TVTVVFYSNRM*ELESLDKVRLCHLPIQTFTKLY LEITCPFSFTGYIKGRAIISKYFTPLTK*AACV EIS*IH*IIILPVFSTR*N**QVFFFCYQALFI KTGDSE*PNADQFYAG***IPF*LYCKLLYRCHI SITKFQQLILVQMFKIWLF*LDIVFFLSLLEGT LIAAK*CCFFAKKLNDLEGAS*LYIIDIKKQTV INNQDVKVIHVGHN*VFKSVYWVLGL*NT*ILDF *FVHGYSTFLESSYQQDGPISKFLVVCTDLLKC ERFLTAS*GRNQYFSGCCISLL*GLFLYCHIHN ADTYM*VVHVAEKGYTYHGFSLILYS*TVFLMLQ AILFFHCT*THCLFKMLLKL*RV*PKQ**TMTV KM*RL*KLHWKGAFKMVGY*LTELLHLKIS*IN SLSILQDKCKLFQAVKVKPNNHYQGNSWVSNT*N HQEWFKGLAIPIPL*STEKSY*KKNTTW**NLS ID*VLYVLPASFQFCC*SFRLYE**SPMFIK*H LNS*TKRKCLLCLKFWGDTK*MCKSNFEVQ*VK* YRPRDHCFTILLSNE*ERLLVAERRKGEKQAMK *SCFPDLGLGHHFKIKDV*EMNQLIDPYFPSKS FPKKCYRF*ITCQ*PSRSKMANQDG*FSHIE*LT FTLTWKVGIYC*MISRSL*QSLTLFFLGFYCFL VSWCLSSELKHVLHP*DDSQGLSFIKSQH*CRV KSTVFAYDFKIFL*FEFALVCAC*EEVSVETTLI CKLGVGDTWWFFKNKDSIFFFFLNTHYHLLLKQ LCQFKWHIKCEQTPHYMKSKW*RASVKHLKSIL CVVLLFIITY*MQ*C*L

Sequence TLK1.gAug05

TLK1.u.f.12 (forward primer)
TLK1.u.r.11 (reverse primer)
Single exon skipping

Frame 0

A*RRRRL*LMQRLPHRLSRHPQPAAAASRATPG ADALQRCRQVPPAEVSSAGAARAPPALRPRPGWH PLDASAAALLSSSSPLARPLAPSLLPLSLLPVP SPSLTSFLPTSPLLGFLPHPLDSPLPALALSLA LSAAPAMTEAGAGAVAAAAVAGGESGSQKVA**V SKVAVEVWRGRHLGPSSPRLQPRARRRRPGPC* ITRRHPGGPGKVQWMSFIVWIQEGKSYWKLDLL ELQVGALEVRAVAVLELKPQQITKALITVLEAWD L*VTKNQRNNWQAAERTVQVTGLYLQ*VPLRHR RRNNRNHPGEEREKQKTRMKVVREKVLGDVATK LATILNTRVEMAQVQ*EAYLLQSVLLKIHIHIPL LPHLFDRIALLLLH*HLG

Frame 1

RDAAAACN*CRGYPTASAATHSPPPPPPGRRRG PTHCRGAGRFRPRKCPRPAQPALRPRSDPGPAG TPSTPPPPRSFPPPLLWRARSLPRFSPFPSSLSP LLPSPLSSPPPLFSVSSPIPLTLPSQPSLSRSP SARPPP*RRRVPVPLPPLPSQGGSRVPRK*LDE CPK*QWKFGGAAILVPALHVSNPGLGGGGQVPAE SHAAIREAQGRCNG*AS*SGSKKARVIGS*IYW SCKWEHWKYGQLQCWS*SLNK*RKL*SQFWKLG IFK*QRIRETTGRQLKGLCK*RDFICSRFP*DTG EETIGIIQGKKEKSRKPE*K*SGKKYWGTWPQN *RLF*IPGWKWLKSSKRHTSCNPFSSKFTFTFH SFLICSTE*PFSYCISIW

Frame 2

VTPPPPVTDAEVTPPPQPPPTARRRRLPGDAGG RRTAEVPAGSARGSVLGRRSPRSARAPTPARLA PPRRLRRRAPFLLLSSGAPARSLASPPFPPPCP LSFPHLFPPHLPSSRFPPPSP*LSPPSPRSLARP QRGPRHDGGGCRCRCRRCRRRGGVGFPESSLMS VQSSSGSLEGPPSWSQLSTSPTPGSAAAARSLL NHTPPSGRPREGAMDELHSLDPRRQELLEARFTG VASGSTGSTGSCSVGAKASTNNESSNHSFGSLG SLSDKESEKQLAGS*KDCASDGTLSAVGSLETP EKKQSESSRGRKRKAENQNESSQGKSIGGRGHKI SDYFEYQGGNGSSPVRGIPPAIRSPQNSHSHST PSSSVRPNSPSPTALAFG

Sequence TLK1.aAug05

GCGTGACGCCGCCGCCGCCTGTAACTGATGCAGAGGTTACCCCACCGCCTCAGCCGCCACCCACAGCCCGCCGCCGCCGCCTCCCGGGCGACGCCGGGGG CCGACGCACTGCAGAGGTGCCGGCAGGTTCCGCCCGCGGAAGTGTCCTCGGCCGGCGCAGCCCGCGCTCCGCCCGCGCTCCGACCCCGGCCCGGCTGGCA CCCCCTCGACGCCTCCGCCGCCGCGCTCCTTTCCTCCTCCTCTCCTCTGGCGCGCCCGCTCGCTCCCTCGCTTCTCCCCCTTTCCCTCCTCCCTGTCCCC TCTCCTTCCCTCACCTCTTTCCTCCCCACCTCCCCTCTTCTCGGTTTCCTCCCCCATCCCCTTGACTCTCCCCTCCCAGCCCTCGCTCTCTCGCTCGCCC TCAGCGCGGCCCCCGCCATGACGGAGGCGGGTGCCGGTGCCGTTGCCGCCGCTGCCGTCGCAGGGGGGGAGTCGGGTTCCCAGAAAGTAGCTTGATGAGT GTCCAAAGTAGCAGTGGAAGTTTGGAGGGGCCGCCATCTTGGTCCCAGCTCTCCACGTCTCCAACCCCGGGCTCGGCGGCGGCGGCCAGGTCCCTGCTGA ATCACACGCCGCCATCCGGGAGGCCCAGGGAAGGTGCAATGGATGAGCTTCATAGTCTGGATCCAAGAAGGCAAGAGTTATTGGAAGCTAGATTTACTGG AGTTGCAAGTGGGAGCACTGGAAGTACGGGCAGTTGCAGTGTTGGAGCTAAAGCCTCAACAAATAACGAAAGCTCTAATCACAGTTTTGGAAGCTTGGGA TCTTTAAGTGACAAAGAATCAGAGACACCGGAGAAGAAACAATCGGAATCATCCAGGGGAAGAAAGAGAAAAGCAGAAAACCAGAATGAAAGTAGTCAGG GGTTCCCCAACCTCCCGGTCTTCCAGTCCTTGGCCTATTGGGAAATGGGTCGTACAGCAGGAGGAAAAAGTATTGGGGGACGTGGCCACAAAATTAGCGA CTATTTTGAATACCAGGGTGGAAATGGCTCAAGTCCAGTAAGAGGCATACCTCCTGCAATCCGTTCTCCTCAAAATTCACATTCACATTCCACTCCTTCC TCATCTGTTCGACCGAATAGCCCTTCTCCTACTGCATTAGCATTTGGGGACCACCCTATTGTACAACCAAAGCAATTATCCTTTAAAATTATTCAGACTG ATCTCACAATGCTGAAATTAGCAGCATTAGAAAGTAATAAAATCCAGGACCTGGAAAAGAAGGAGGGACGTATAGATGATTTGCTCAGGGCTAACTGTGA TCTCAGACGGCAAATAGATGAACAACAAAAATTACTTGAAAAATACAAAGAACGATTAAATAAGTGCATATCAATGAGCAAGAAACTTCTTATTGAAAAG AGTACACAAGAAAAGCTGTCAAGCAGAGAGAAGAGTATGCAAGATCGATTACGCCTCGGGCACTTTACAACAGTTAGACATGGCGCTTCATTTACTGAAC AATGGACAGATGGTTTTGCATTTCAGAATCTTGTGAAGCAACAAGAATGGGTGAATCAGCAAAGGGAAGATATTGAAAGGCAAAGGAAACTTCTAGCCAA ACGCAAACCTCCCACAGCTAATAATTCTCAGGCACCCTCTACCAATTCTGAACCAAAACAAAGGAAAAACAAAGCAGTCAATGGAGCAGAGAATGATCCC TTTGTTAGACCAAATTTACCACAACTGTTGACTTTGGCAGAATATCATGAACAGGAAGAAATTTTCAAACTTAGACTAGGACATCTCAAAAAGGAAGAGG CAGAAATCCAGGCAGAACTTGAACGTTTGGAAAGAGTCAGAAATCTTCACATACGTGAGCTGAAAAGAATAAACAATGAAGATAATTCACAGTTCAAAGA TCACCCAACATTAAATGAAAGATATTTATTACTTCATCTGCTTGGTAGAGGTGGCTTTAGTGAAGTGTATAAGGCTTTTGACCTTTATGAACAAAGATAT GCTGCTGTGAAGATACATCAGCTTAATAAAAGCTGGAGAGATGAGAAGAAAGAAAACTACCACAAACATGCCTGCAGAGAGTATAGAATACACAAAGAAC TGGATCACCCCAGAATAGTTAAACTCTATGATTATTTCTCCTTGGATACAGATACGTTTTGTACAGTGTTAGAATACTGTGAAGGCAATGACTTGGATTT CTATCTGAAGCAACACAAGTTAATGTCAGAGAAAGAAGCTCGGTCTATTGTAATGCAGATTGTAAATGCACTAAGATATCTCAATGAGATCAAACCCCCT ATTATACATTATGATCTTAAGCCAGGAAACATCCTACTGGTAGATGGAACAGCATGTGGTGAAATCAAAATCACTGATTTTGGTCTGTCCAAGATTATGG ATGATGATAGCTATGGTGTAGATGGAATGGATCTAACTTCCCAGGGGGCAGGCACTTACTGGTATTTACCTCCTGAGTGTTTTGTAGTTGGAAAAGAGCC ACCAAAGATTTCCAACAAGGTTGATGTATGGTCGGTTGGAGTCATCTTCTTTCAGTGTCTTTATGGTAGAAAGCCATTTGGTCACAATCAATCTCAACAA GACATTCTTCAAGAAAATACAATATTAAAAGCCACAGAAGTCCAGTTCCCTGTAAAACCGGTTGTAAGCAGTGAAGCCAAGGCATTTATAAGACGCTGTT TGGCATATCGAAAAGAAGATCGATTTGATGTGCACCAGCTGGCAAATGACCCATACCTTCTCCCACACATGAGAAGATCAAATTCTTCAGGAAACCTACA CATGGCTGGGCTGACAGCATCCCCTACACCCCCTTCTTCAAGCATAATTACTTACTGACTTTCCTCCAAGATTGGCATGATATCTTTGAATTTGCTTCCA GATGCACACTTAAGTTTGAGAGCATTTGAGTGTTTGTTTTCTTTTTCTTTTTTTTTTTTTTTTTTTTTTACACAAGACGTGGTTAAGAACTGTTTGTGAA CTGAAGTTCCTCATAGTGTCATTTGTATGAGAGAGGATCATGGACAATGAATAAATGCACACTTCTGACTATAATTTTGAGCAGTGAAGGAAGGATGACT GCCTGTTACACAGAAACAGGAATACCATGTTAAGATAGAGGAAAAAAATTTTGTTTATTTGGGGGAACACTGTAAATAACGTTTATAATACTTAAATACA TTTGGTTGTTACTAGAGAGTTCTAATATGAAGGGTAGTTTTTCATTATTTAAAAACACATGGAAAAATATGAGACATATTTCTAACATACGAACTATAGT GCCTGGATACATTCTTCAGCATTTGGCAGTTAATCTGCTGAAACCAAAGTAAGAACTGAATGACAGTGACAGTTGTGTTTTATAGTAATAGAATGTGAGA GTTAGAATCTTTGGACAAAGTTAGGCTTTGTCATTTGCCTATTCAGACTTTTACCAAGTTGTACCTCGAGATCACATGTCCGTTTTCTTTCACTGGTTAT ATTAAAGGGAGAGCTATTATTTCTAAATACTTTACACCTTTGACAAAATGAGCTGCTTGTGTTGAAATCAGTTGAATTCACTAAATTATAATATTGCCAG TGTTTTCAACACGTTAGAATTGATAACAGGTATTTTTCTTTTGTTACCAGGCCTTGTTCATCAAAACAGGTGACAGTGAGTAACCAAATGCAGACCAGTT CTATGCAGGATAATGATAAATTCCCTTCTAGCTCTATTGTAAATTGTTGTACAGATGTCATATTTCAATTACTAAGTTTCAGCAGCTTATTCTTGTACAA ATGTTTAAAATATGGCTTTTCTAATTGGATATTGTATTTTTTTTAAGTCTTCTTGAAGGCACTCTAATTGCTGCTAAATGATGTTGCTTCTTTGCTAAAA AATTAAATGACCTAGAAGGTGCAAGTTGACTGTACATCATAGATATTAAAAAGCAGACAGTCATTAACAATCAAGATGTAAAAGTGATCCATGTTGGACA TAATTGAGTTTTTAAATCAGTTTATTGGGTTCTAGGGCTGTAGAACACATAGATACTAGATTTTTAATTTGTTCATGGTTATTCTACATTTCTAGAAAGT TCTTATCAGCAAGATGGTCCCATAAGCAAATTTCTTGTGGTTTGTACAGATTTGTTAAAATGTGAACGTTTTCTAACTGCCTCGTAGGGTAGAAACCAAT ATTTTTCAGGATGCTGTATTTCACTCCTGTAAGGACTTTTTCTTTATTGTCACATTCATAATGCTGATACTTACATGTAAGTTGTTCATGTTGCAGAAAA AGGTTATACGTACCACGGATTCTCTTTAATATTGTACAGTTAAACTGTATTTCTGATGTTGCAAGCCATTTTGTTTTTTCATTGTACATAGACACATTGT CTCTTTAAAATGCTGCTGAAATTGTAGAGAGTATAGCCAAAACAGTAATAAACAATGACAGTTAAAATGTAAAGACTATGAAAATTACATTGGAAGGGAG CTTTCAAGATGGTAGGATATTGACTAACTGAGCTCCTTCACTTAAAAATCAGCTGAATAAATTCCTTAAGTATACTTCAAGACAAGTGTAAATTGTTTCA AGCTGTTAAAGTTAAACCTAATAACCATTATCAAGGCAACTCCTGGGTAAGTAACACTTAGAACCATCAAGAGTGGTTTAAGGGGCTGGCTATACCCATT CCTCTGTAGAGCACAGAGAAATCCTATTAAAAAAAAAATACTACATGGTAATGAAATCTTAGCATTGATTGAGTGCTTTACGTGCTGCCTGCAAGTTTTC AGTTTTGTTGTTAGAGTTTTCGTCTTTATGAATGATAAAGTCCTATGTTCATAAAGTAACATTTAAATTCCTGAACTAAAAGGAAATGCCTGTTGTGCTT AAAATTTTGGGGAGATACGAAGTGAATGTGTAAATCTAACTTTGAGGTTCAGTAAGTTAAGTAGTATAGACCTAGAGATCACTGCTTTACCATTTTACTT TCAAATGAATGAGAGCGACTGCTAGTGGCAGAACGCAGAAAAGGGGAAAAACAAGCCATGAAATGAAGTTGCTTTCCTGACCTGGGTTTAGGTCATCATT TTAAAATCAAAGATGTGTAGGAGATGAATCAACTTATAGACCCATATTTTCCTTCTAAAAGTTTCCCAAAGAAATGTTATAGATTTTGAATAACATGCCA GTGACCCAGCAGGTCCAAGATGGCTAACCAAGATGGCTAATTTAGCCACATTGAATAACTTACTTTTACCCTCACATGGAAAGTTGGTATCTATTGCTAA ATGATTTCAAGGTCACTCTGACAGTCTTTGACACTGTTTTTTTTAGGGTTTTATTGTTTTCTGGTCTCCTGGTGTCTGTCCTCAGAATTAAAGCATGTCC TGCATCCCTAAGATGATAGCCAAGGCCTCTCTTTTATTAAGAGTCAGCACTGATGTAGGGTAAAATCAACAGTTTTTGCATATGATTTTAAAATCTTTCT TTGATTTGAATTTGCTTTAGTGTGTGCATGTTGAGAGGAAGTAAGTGTAGAAACCACCCTAATTTGTAAGTTGGGAGTTGGAGATACTTGGTGGTTTTTC AAAAATAAAGACTCAATCTTTTTTTTTTTTTTAAACACTCACTACCATCTCCTTCTAAAACAACTCTGTCAGTTTAAATGGCATATAAAATGTGAACAAA CACCTCATTACATGAAGTCAAAATGGTGAAGAGCTTCAGTGAAACATTTAAAGAGTATATTGTGTGTTGTTTTGCTGTTCATCATTACTTATTAAATGCA GTAATGTTGATTGCC

TLK1.u.f.12 (forward primer)
TLK1.u.r.11 (reverse primer)
Single exon skipping

Frame 0

A*RRRRL*LMQRLPHRLSRHPQPAAAASRATPG ADALQRCRQVPPAEVSSAGAARAPPALRPRPGWH PLDASAAALLSSSSPLARPLAPSLLPLSLLPVP SPSLTSFLPTSPLLGFLPHPLDSPLPALALSLA LSAAPAMTEAGAGAVAAAAVAGGESGSQKVA**V SKVAVEVWRGRHLGPSSPRLQPRARRRRPGPC* ITRRHPGGPGKVQWMSFIVWIQEGKSYWKLDLL ELQVGALEVRAVAVLELKPQQITKALITVLEAWD L*VTKNQRHRRRNNRNHPGEEREKQKTRMKVVR GSPTSRSSSPWPIGKWVVQQEEKVLGDVATKLA TILNTRVEMAQVQ*EAYLLQSVLLKIHIHIPLLP HLFDRIALLLLH*HLGTTLLYNQSNYPLKLFRL ISQC*N*QH*KVIKSRTWKRRRDV*MICSGLTV ISDGK*MNNKNYLKNTKND*ISAYQ*ARNFLLKR VHKKSCQAERRVCKIDYASGTLQQLDMALHLLN NGQMVLHFRIL*SNKNG*ISKGKILKGKGNF*P NANLPQLIILRHPLPILNQNKGKTKQSMEQRMIP LLDQIYHNC*LWQNIMNRKKFSNLD*DISKRKR QKSRQNLNVWKESEIFTYVS*KE*TMKIIHSSK ITQH*MKDIYYFICLVEVALVKCIRLLTFMNKDM LL*RYISLIKAGEMRRKKTTTNMPAESIEYTKN WITPE*LNSMIISPWIQIRFVQC*NTVKAMTWI SI*SNTS*CQRKKLGLL*CRL*MH*DISMRSNPL LYIMILSQETSYW*MEQHVVKSKSLILVCPRLW MMIAMV*MEWI*LPRGQALTGIYLLSVL*LEKS HQRFPTRLMYGRLESSSFSVFMVESHLVTINLNK TFFKKIQY*KPQKSSSL*NRL*AVKPRHL*DAV WHIEKKIDLMCTSWQMTHTFSHT*EDQILQETY TWLG*QHPLHPLLQA*LLTDFPPRLA*YL*ICFQ MHT*V*EHLSVCFLFLFFFFFFLHKTWLRTVCE LKFLIVSFV*ERIMDNE*MHTSDYNFEQ*RKDD CLLHRNRNTMLR*RKKILFIWGNTVNNVYNT*IH LVVTREF*YEG*FFII*KHMEKYETYF*HTNYS AWIHSSAFGS*SAETKVRTE*Q*QLCFIVIECE S*NLWTKLGFVICLFRLLPSCTSRSHVRFLSLVI LKGELLFLNTLHL*QNELLVLKSVEFTKL*YCQ CFQHVRIDNRYFSFVTRPCSSKQVTVSNQMQTS SMQDNDKFPSSSIVNCCTDVIFQLLSFSSLFLYK CLKYGFSNWILYFF*VFLKAL*LLLNDVASLLK N*MT*KVQVDCTS*ILKSRQSLTIKM*K*SMLD IIEFLNQFIGF*GCRTHRY*IFNLFMVILHF*KV LISKMVP*ANFLWFVQIC*NVNVF*LPRRVETN IFQDAVFHSCKDFFFIVTFIMLILTCKLFMLQK KVIRTTDSL*YCTVKLYF*CCKPFCFFIVHRHIV SLKCC*NCREYSQNSNKQ*QLKCKDYENYIGRE LSRW*DID*LSSFT*KSAE*IP*VYFKTSVNCF KLLKLNLITIIKATPG*VTLRTIKSGLRGWLYPF LCRAQRNPIKKKILHGNEILALIECFTCCLQVF SFVVRVFVFMNDKVLCS*SNI*IPELKGNACCA *NFGEIRSECVNLTLRFSKLSSIDLEITALPFYF QMNESDC*WQNAEKGKNKP*NEVAFLTWV*VII LKSKMCRR*INL*THIFLLKVSQRNVIDFE*HA SDPAGPRWLTKMANLATLNNLLLPSHGKLVSIAK *FQGHSDSL*HCFF*GFIVFWSPGVCPQN*SMS CIPKMIAKASLLLRVSTDVG*NQQFLHMILKSF FDLNLL*CVHVERK*V*KPP*FVSWELEILGGFS KIKTQSFFFF*TLTTISF*NNSVSLNGI*NVNK HLIT*SQNGEELQ*NI*RVYCVLFCCSSLLIKC SNVDC

Frame 1

RDAAAACN*CRGYPTASAATHSPPPPPPGRRRG PTHCRGAGRFRPRKCPRPAQPALRPRSDPGPAG TPSTPPPPRSFPPPLLWRARSLPRFSPFPSSLSP LLPSPLSSPPPLFSVSSPIPLTLPSQPSLSRSP SARPPP*RRRVPVPLPPLPSQGGSRVPRK*LDE CPK*QWKFGGAAILVPALHVSNPGLGGGGQVPAE SHAAIREAQGRCNG*AS*SGSKKARVIGS*IYW SCKWEHWKYGQLQCWS*SLNK*RKL*SQFWKLG IFK*QRIRDTGEETIGIIQGKKEKSRKPE*K*SG VPQPPGLPVLGLLGNGSYSRRKKYWGTWPQN*R LF*IPGWKWLKSSKRHTSCNPFSSKFTFTFHSF LICSTE*PFSYCISIWGPPYCTTKAIIL*NYSD* SHNAEISSIRK**NPGPGKEGGTYR*FAQG*L* SQTANR*TTKIT*KIQRTIK*VHINEQETSY*K EYTRKAVKQREEYARSITPRALYNS*TWRFIY*T MDRWFCISESCEATRMGESAKGRY*KAKETSSQ TQTSHS**FSGTLYQF*TKTKEKQSSQWSRE*S LC*TKFTTTVDFGRIS*TGRNFQT*TRTSQKGRG RNPGRT*TFGKSQKSSHT*AEKNKQ*R*FTVQR SPNIK*KIFITSSAW*RWL**SV*GF*PL*TKI CCCEDTSA**KLER*EERKLPQTCLQRV*NTQRT GSPQNS*TL*LFLLGYRYVLYSVRIL*RQ*LGF LSEATQVNVRERSSVYCNADCKCTKISQ*DQTP YYTL*S*ARKHPTGRWNSMW*NQNH*FWSVQDYG ***LWCRWNGSNFPGGRHLLVFTS*VFCSWKRA TKDFQQG*CMVGWSHLLSVSLW*KAIWSQSIST RHSSRKYNIKSHRSPVPCKTGCKQ*SQGIYKTLF GISKRRSI*CAPAGK*PIPSPTHEKIKFFRKPT HGWADSIPYTPFFKHNYLLTFLQDWHDIFEFAS RCTLKFESI*VFVFFFFFFFFFFYTRRG*ELFVN *SSS*CHLYERGSWTMNKCTLLTIILSSEGRMT ACYTETGIPC*DRGKKFCLFGGTL*ITFIILKY IWLLLESSNMKGSFSLFKNTWKNMRHISNIRTIV PGYILQHLAVNLLKPK*ELNDSDSCVL***NVR VRIFGQS*ALSFAYSDFYQVVPRDHMSVFFHWL Y*RESYYF*ILYTFDKMSCLC*NQLNSLNYNIAS VFNTLELITGIFLLLPGLVHQNR*Q*VTKCRPV LCRIMINSLLALL*IVVQMSYFNY*VSAAYSCT NV*NMAFLIGYCIFFKSS*RHSNCC*MMLLLC*K IK*PRRCKLTVHHRY*KADSH*QSRCKSDPCWT *LSF*ISLLGSRAVEHIDTRFLICSWLFYISRK FLSARWSHKQISCGLYRFVKM*TFSNCLVG*KPI FFRMLYFTPVRTFSLLSHS*C*YLHVSCSCCRK RLYVPRILFNIVQLNCISDVASHFVFSLYIDTL SL*NAAEIVESIAKTVINNDS*NVKTMKITLEGS FQDGRILTN*APSLKNQLNKFLKYTSRQV*IVS SC*S*T**PLSRQLLGK*HLEPSRVV*GAGYTH SSVEHREILLKKKYYMVMKS*H*LSALRAACKFS VLLLEFSSL*MIKSYVHKVTFKFLN*KEMPVVL KILGRYEVNV*I*L*GSVS*VV*T*RSLLYHFT FK*MRATASGRTQKRGKTSHEMKLLS*PGFRSSF *NQRCVGDESTYRPIFSF*KFPKEML*ILNNMP VTQQVQDG*PRWLI*PH*ITYFYPHMESWYLLL NDFKVTLTVFDTVFFRVLLFSGLLVSVLRIKACP ASLR**PRPLFY*ESALM*GKINSFCI*F*NLS LI*ICFSVCMLRGSKCRNHPNL*VGSWRYLVVF QK*RLNLFFFFKHSLPSPSKTTLSV*MAYKM*TN TSLHEVKMVKSFSETFKEYIVCCFAVHHYLLNA VMLIA

Frame 2

VTPPPPVTDAEVTPPPQPPPTARRRRLPGDAGG RRTAEVPAGSARGSVLGRRSPRSARAPTPARLA PPRRLRRRAPFLLLSSGAPARSLASPPFPPPCP LSFPHLFPPHLPSSRFPPPSP*LSPPSPRSLARP QRGPRHDGGGCRCRCRRCRRRGGVGFPESSLMS VQSSSGSLEGPPSWSQLSTSPTPGSAAAARSLL NHTPPSGRPREGAMDELHSLDPRRQELLEARFTG VASGSTGSTGSCSVGAKASTNNESSNHSFGSLG SLSDKESETPEKKQSESSRGRKRKAENQNESSQ GFPNLPVFQSLAYWEMGRTAGGKSIGGRGHKISD YFEYQGGNGSSPVRGIPPAIRSPQNSHSHSTPS SSVRPNSPSPTALAFGDHPIVQPKQLSFKIIQT DLTMLKLAALESNKIQDLEKKEGRIDDLLRANCD LRRQIDEQQKLLEKYKERLNKCISMSKKLLIEK STQEKLSSREKSMQDRLRLGHFTTVRHGASFTE QWTDGFAFQNLVKQQEWVNQQREDIERQRKLLAK RKPPTANNSQAPSTNSEPKQRKNKAVNGAENDP FVRPNLPQLLTLAEYHEQEEIFKLRLGHLKKEE AEIQAELERLERVRNLHIRELKRINNEDNSQFKD HPTLNERYLLLHLLGRGGFSEVYKAFDLYEQRY AAVKIHQLNKSWRDEKKENYHKHACREYRIHKE LDHPRIVKLYDYFSLDTDTFCTVLEYCEGNDLDF YLKQHKLMSEKEARSIVMQIVNALRYLNEIKPP IIHYDLKPGNILLVDGTACGEIKITDFGLSKIM DDDSYGVDGMDLTSQGAGTYWYLPPECFVVGKEP PKISNKVDVWSVGVIFFQCLYGRKPFGHNQSQQ DILQENTILKATEVQFPVKPVVSSEAKAFIRRC LAYRKEDRFDVHQLANDPYLLPHMRRSNSSGNLH MAGLTASPTPPSSSIITY*LSSKIGMISLNLLP DAHLSLRAFECLFSFSFFFFFFFTQDVVKNCL* TEVPHSVICMREDHGQ*INAHF*L*F*AVKEG*L PVTQKQEYHVKIEEKNFVYLGEHCK*RL*YLNT FGCY*RVLI*RVVFHYLKTHGKI*DIFLTYEL* CLDTFFSIWQLIC*NQSKN*MTVTVVFYSNRM*E LESLDKVRLCHLPIQTFTKLYLEITCPFSFTGY IKGRAIISKYFTPLTK*AACVEIS*IH*IIILP VFSTR*N**QVFFFCYQALFIKTGDSE*PNADQF YAG***IPF*LYCKLLYRCHISITKFQQLILVQ MFKIWLF*LDIVFFLSLLEGTLIAAK*CCFFAK KLNDLEGAS*LYIIDIKKQTVINNQDVKVIHVGH N*VFKSVYWVLGL*NT*ILDF*FVHGYSTFLES SYQQDGPISKFLVVCTDLLKCERFLTAS*GRNQ YFSGCCISLL*GLFLYCHIHNADTYM*VVHVAEK GYTYHGFSLILYS*TVFLMLQAILFFHCT*THC LFKMLLKL*RV*PKQ**TMTVKM*RL*KLHWKG AFKMVGY*LTELLHLKIS*INSLSILQDKCKLFQ AVKVKPNNHYQGNSWVSNT*NHQEWFKGLAIPI PL*STEKSY*KKNTTW**NLSID*VLYVLPASF QFCC*SFRLYE**SPMFIK*HLNS*TKRKCLLCL KFWGDTK*MCKSNFEVQ*VK*YRPRDHCFTILL SNE*ERLLVAERRKGEKQAMK*SCFPDLGLGHH FKIKDV*EMNQLIDPYFPSKSFPKKCYRF*ITCQ *PSRSKMANQDG*FSHIE*LTFTLTWKVGIYC* MISRSL*QSLTLFFLGFYCFLVSWCLSSELKHV LHP*DDSQGLSFIKSQH*CRVKSTVFAYDFKIFL *FEFALVCAC*EEVSVETTLICKLGVGDTWWFF KNKDSIFFFFLNTHYHLLLKQLCQFKWHIKCEQ TPHYMKSKW*RASVKHLKSILCVVLLFIITY*MQ *C*L