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Sequences

Sequence KLK7.aAug05

AGATAACCAGAACCGCAAATGCAGGCGGGGGTGTCCCAGAGTCGGCTCCGCCTGCACCCCAGGGCGCTGGGGCCGGGCATGGGGCGGGGGGTGATATAAG
AGGACGGCCCAGCAGAGGGATGAAGATTTTGGAGCCCAGCTGTGTGCCAGCCCAAGTCGGAACTTGGATCACATCAGATCCTCTCGAGCCCAGGGTGACA
AGATTATTGATGGCGCCCCATGTGCAAGAGGCTCCCACCCATGGCAGGTGGCCCTGCTCAGTGGCAATCAGCTCCACTGCGGAGGCGTCCTGGTCAATGA
GCGCTGGGTGCTCACTGCCGCCCACTGCAAGATGAATGAGTACACCGTGCACCTGGGCAGTGATACGCTGGGCGACAGGAGAGCTCAGAGGATCAAGGCC
TCGAAGTCATTCCGCCACCCCGGCTACTCCACACAGACCCATGTTAATGACCTCATGCTCGTGAAGCTCAATAGCCAGGCCAGGCTGTCATCCATGGTGA
AGAAAGTCAGGCTGCCCTCCCGCTGCGAACCCCCTGGAACCACCTGTACTGTCTCCGGCTGGGGCACTACCACGAGCCCAGATGTGACCTTTCCCTCTGA
CCTCATGTGCGTGGATGTCAAGCTCATCTCCCCCCAGGACTGCACGAAGGTTTACAAGGACTTACTGGAAAATTCCATGCTGTGCGCTGGCATCCCCGAC
TCCAAGAAAAACGCCTGCAATGGTGACTCAGGGGGACCGTTGGTGTGCAGAGGTACCCTGCAAGGTCTGGTGTCCTGGGGAACTTTCCCTTGCGGCCAAC
CCAATGACCCAGGAGTCTACACTCAAGTGTGCAAGTTCACCAAGTGGATAAATGACACCATGAAAAAGCATCGCTAACGCCACACTGAGTTAATTAACTG
TGTGCTTCCAACAGAAAATGCACAGGAGTGAGGACGCCGATGACCTATGAAGTCAAATTTGACTTTACCTTTCCTCAAAGATATATTTAAACCAACCTCA
TGCCCTGTTGATAAACCAATCAAATTGGTAAAGACCTAAAACCAAAACAAATAAAGAAACACAAAACCCTCAGTGCTGGAGAAGAGTCAGTGAGACCAGC
ACTCTCAAACACTGGAACTGGACGTTCGTACAGTCTTTACGGAAGACACTTGGTCAACGTACACCGAGACCCTTATTCACCACCTTTGACCCAGTAACTC
TAATCTTAGGAAGAACCTACTGAAACAAAAAAAATCCAAAATGTAGAACAAGACTTGAATTTACCATGATATTATTTATCACAGAAATGAAGTGAAACCA
TCAAACATGTTCCAAAAGTACCAGATGGCTTAAATAATAGTCTGGCTTGGCACAACGATGTTTTTTTTCTTTGAGACAGAGTCTCTGTTGCTTGGGCTGC
AATGCAGTGATGCAATCTTGGCTCACTGCAACCTCCGCCTCCTGGGTTCAAGTGATTCTCGTGCTTCAGCCTCCCAAGTACCTGGGACTACAGGTGTGCA
CCACCACACCAGGCTAATTTTTTGTGTATTTTTACTAGAGACAGGGTTTCACCATGTTGGCCAGCATGGTCTTGAACGCCTGACCTCAGATGATCCACCC
ACCTTGGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACCACGGCCAGCCCACAATGATATTACAAACCTATTAAAAATGATACTTAGACAGAATTG
TCAGTATTATTCAAGAACATTTAGGCTATAGGATGTTAAATGACAAAAGGAAGGACAAAAATATATATGTATGTGACCCTACCCATAAAAAATGAAATAT
TCACAGAATCAGATCTGAAAACACATGTCCCAGACTGCATACTGGGGTCGTCATGAGGTGTCTCCTTCCTTCTGTGTACTTTTCCTTGAATGTGCACTTT
TATAACATGAAAAATAAAGGTGGGGAAAAAAGTCTGAAGA
  • KLK7.u.f.1 (forward primer)
  • KLK7.u.r.2 (reverse primer)
  • Single exon skipping

Frame 0

R*PEPQMQAGVSQSRLRLHPRALGPGMGRGVI*
EDGPAEG*RFWSPAVCQPKSELGSHQILSSPG*Q
DY*WRPMCKRLPPMAGGPAQWQSAPLRRRPGQ*
ALGAHCRPLQDE*VHRAPGQ*YAGRQESSEDQG
LEVIPPPRLLHTDPC**PHAREAQ*PGQAVIHGE
ESQAALPLRTPWNHLYCLRLGHYHEPRCDLSL*
PHVRGCQAHLPPGLHEGLQGLTGKFHAVRWHPR
LQEKRLQW*LRGTVGVQRYPARSGVLGNFPLRPT
Q*PRSLHSSVQVHQVDK*HHEKASLTPH*VN*L
CASNRKCTGVRTPMTYEVKFDFTFPQRYI*TNL
MPC**TNQIGKDLKPKQIKKHKTLSAGEESVRPA
LSNTGTGRSYSLYGRHLVNVHRDPYSPPLTQ*L
*S*EEPTETKKIQNVEQDLNLP*YYLSQK*SET
IKHVPKVPDGLNNSLAWHNDVFFL*DRVSVAWAA
MQ*CNLGSLQPPPPGFK*FSCFSLPSTWDYRCA
PPHQANFLCIFTRDRVSPCWPAWS*TPDLR*ST
HLGLPKCWDYRHEPPRPAHNDITNLLKMILRQNC
QYYSRTFRL*DVK*QKEGQKYICM*PYP*KMKY
SQNQI*KHMSQTAYWGRHEVSPSFCVLFLECAL
L*HEK*RWGKKSE

Frame 1

DNQNRKCRRGCPRVGSACTPGRWGRAWGGG*YK
RTAQQRDEDFGAQLCASPSRNLDHIRSSRAQGD
KIIDGAPCARGSHPWQVALLSGNQLHCGGVLVNE
RWVLTAAHCKMNEYTVHLGSDTLGDRRAQRIKA
SKSFRHPGYSTQTHVNDLMLVKLNSQARLSSMV
KKVRLPSRCEPPGTTCTVSGWGTTTSPDVTFPSD
LMCVDVKLISPQDCTKVYKDLLENSMLCAGIPD
SKKNACNGDSGGPLVCRGTLQGLVSWGTFPCGQ
PNDPGVYTQVCKFTKWINDTMKKHR*RHTELINC
VLPTENAQE*GRR*PMKSNLTLPFLKDIFKPTS
CPVDKPIKLVKT*NQNK*RNTKPSVLEKSQ*DQ
HSQTLELDVRTVFTEDTWSTYTETLIHHL*PSNS
NLRKNLLKQKKSKM*NKT*IYHDIIYHRNEVKP
SNMFQKYQMA*IIVWLGTTMFFFFETESLLLGL
QCSDAILAHCNLRLLGSSDSRASASQVPGTTGVH
HHTRLIFCVFLLETGFHHVGQHGLERLTSDDPP
TLASQSAGITGMSHHGQPTMILQTY*K*YLDRI
VSIIQEHLGYRMLNDKRKDKNIYVCDPTHKK*NI
HRIRSENTCPRLHTGVVMRCLLPSVYFSLNVHF
YNMKNKGGEKSLK

Frame 2

ITRTANAGGGVPESAPPAPQGAGAGHGAGGDIR
GRPSRGMKILEPSCVPAQVGTWITSDPLEPRVT
RLLMAPHVQEAPTHGRWPCSVAISSTAEASWSM
SAGCSLPPTAR*MSTPCTWAVIRWATGELRGSRP
RSHSATPATPHRPMLMTSCS*SSIARPGCHPW*
RKSGCPPAANPLEPPVLSPAGALPRAQM*PFPL
TSCAWMSSSSPPRTARRFTRTYWKIPCCALASPT
PRKTPAMVTQGDRWCAEVPCKVWCPGELSLAAN
PMTQESTLKCASSPSG*MTP*KSIANATLS*LT
VCFQQKMHRSEDADDL*SQI*LYLSSKIYLNQPH
ALLINQSNW*RPKTKTNKETQNPQCWRRVSETS
TLKHWNWTFVQSLRKTLGQRTPRPLFTTFDPVT
LILGRTY*NKKNPKCRTRLEFTMILFITEMK*NH
QTCSKSTRWLK**SGLAQRCFFSLRQSLCCLGC
NAVMQSWLTATSASWVQVILVLQPPKYLGLQVC
TTTPG*FFVYFY*RQGFTMLASMVLNA*PQMIHP
PWPPKVLGLQA*ATTASPQ*YYKPIKNDT*TEL
SVLFKNI*AIGC*MTKGRTKIYMYVTLPIKNEI
FTESDLKTHVPDCILGSS*GVSFLLCTFP*MCTF
IT*KIKVGKKV*R

Sequence KLK7.bAug05

AGATAACCAGAACCGCAAATGCAGGCGGGGGTGTCCCAGAGTCGGCTCCGCCTGCACCCCAGGGCGCTGGGGCCGGGCATGGGGCGGGGGGTGATATAAG
AGGACGGCCCAGCAGAGGGATGAAGATTTTGGAGCCCAGCTGTGTGCCAGCCCAAGTCGGAACTTGGATCACATCAGATCCTCTCGAGCTCCAGCAGGAG
AGGCCCTTCCTCGCCTGGCAGCCCCTGAGCGGCTCAGCAGGGCACCATGGCAAGATCCCTTCTCCTGCCCCTGCAGATCTTACTGCTATCCTTAGCCTTG
GAAACTGCAGGAGAAGAAGCCCAGGGTGACAAGATTATTGATGGCGCCCCATGTGCAAGAGGCTCCCACCCATGGCAGGTGGCCCTGCTCAGTGGCAATC
AGCTCCACTGCGGAGGCGTCCTGGTCAATGAGCGCTGGGTGCTCACTGCCGCCCACTGCAAGATGAATGAGTACACCGTGCACCTGGGCAGTGATACGCT
GGGCGACAGGAGAGCTCAGAGGATCAAGGCCTCGAAGTCATTCCGCCACCCCGGCTACTCCACACAGACCCATGTTAATGACCTCATGCTCGTGAAGCTC
AATAGCCAGGCCAGGCTGTCATCCATGGTGAAGAAAGTCAGGCTGCCCTCCCGCTGCGAACCCCCTGGAACCACCTGTACTGTCTCCGGCTGGGGCACTA
CCACGAGCCCAGATGTGACCTTTCCCTCTGACCTCATGTGCGTGGATGTCAAGCTCATCTCCCCCCAGGACTGCACGAAGGTTTACAAGGACTTACTGGA
AAATTCCATGCTGTGCGCTGGCATCCCCGACTCCAAGAAAAACGCCTGCAATGGTGACTCAGGGGGACCGTTGGTGTGCAGAGGTACCCTGCAAGGTCTG
GTGTCCTGGGGAACTTTCCCTTGCGGCCAACCCAATGACCCAGGAGTCTACACTCAAGTGTGCAAGTTCACCAAGTGGATAAATGACACCATGAAAAAGC
ATCGCTAACGCCACACTGAGTTAATTAACTGTGTGCTTCCAACAGAAAATGCACAGGAGTGAGGACGCCGATGACCTATGAAGTCAAATTTGACTTTACC
TTTCCTCAAAGATATATTTAAACCAACCTCATGCCCTGTTGATAAACCAATCAAATTGGTAAAGACCTAAAACCAAAACAAATAAAGAAACACAAAACCC
TCAGTGCTGGAGAAGAGTCAGTGAGACCAGCACTCTCAAACACTGGAACTGGACGTTCGTACAGTCTTTACGGAAGACACTTGGTCAACGTACACCGAGA
CCCTTATTCACCACCTTTGACCCAGTAACTCTAATCTTAGGAAGAACCTACTGAAACAAAAAAAATCCAAAATGTAGAACAAGACTTGAATTTACCATGA
TATTATTTATCACAGAAATGAAGTGAAACCATCAAACATGTTCCAAAAGTACCAGATGGCTTAAATAATAGTCTGGCTTGGCACAACGATGTTTTTTTTC
TTTGAGACAGAGTCTCTGTTGCTTGGGCTGCAATGCAGTGATGCAATCTTGGCTCACTGCAACCTCCGCCTCCTGGGTTCAAGTGATTCTCGTGCTTCAG
CCTCCCAAGTACCTGGGACTACAGGTGTGCACCACCACACCAGGCTAATTTTTTGTGTATTTTTACTAGAGACAGGGTTTCACCATGTTGGCCAGCATGG
TCTTGAACGCCTGACCTCAGATGATCCACCCACCTTGGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACCACGGCCAGCCCACAATGATATTACAA
ACCTATTAAAAATGATACTTAGACAGAATTGTCAGTATTATTCAAGAACATTTAGGCTATAGGATGTTAAATGACAAAAGGAAGGACAAAAATATATATG
TATGTGACCCTACCCATAAAAAATGAAATATTCACAGAATCAGATCTGAAAACACATGTCCCAGACTGCATACTGGGGTCGTCATGAGGTGTCTCCTTCC
TTCTGTGTACTTTTCCTTGAATGTGCACTTTTATAACATGAAAAATAAAGGTGGGGAAAAAAGTCTGAAGA
  • KLK7.u.f.1 (forward primer)
  • KLK7.u.r.2 (reverse primer)
  • Single exon skipping

Frame 0

R*PEPQMQAGVSQSRLRLHPRALGPGMGRGVI*
EDGPAEG*RFWSPAVCQPKSELGSHQILSSSSRR
GPSSPGSP*AAQQGTMARSLLLPLQILLLSLAL
ETAGEEAQGDKIIDGAPCARGSHPWQVALLSGN
QLHCGGVLVNERWVLTAAHCKMNEYTVHLGSDTL
GDRRAQRIKASKSFRHPGYSTQTHVNDLMLVKL
NSQARLSSMVKKVRLPSRCEPPGTTCTVSGWGT
TTSPDVTFPSDLMCVDVKLISPQDCTKVYKDLLE
NSMLCAGIPDSKKNACNGDSGGPLVCRGTLQGL
VSWGTFPCGQPNDPGVYTQVCKFTKWINDTMKK
HR*RHTELINCVLPTENAQE*GRR*PMKSNLTLP
FLKDIFKPTSCPVDKPIKLVKT*NQNK*RNTKP
SVLEKSQ*DQHSQTLELDVRTVFTEDTWSTYTE
TLIHHL*PSNSNLRKNLLKQKKSKM*NKT*IYHD
IIYHRNEVKPSNMFQKYQMA*IIVWLGTTMFFF
FETESLLLGLQCSDAILAHCNLRLLGSSDSRAS
ASQVPGTTGVHHHTRLIFCVFLLETGFHHVGQHG
LERLTSDDPPTLASQSAGITGMSHHGQPTMILQ
TY*K*YLDRIVSIIQEHLGYRMLNDKRKDKNIY
VCDPTHKK*NIHRIRSENTCPRLHTGVVMRCLLP
SVYFSLNVHFYNMKNKGGEKSLK

Frame 1

DNQNRKCRRGCPRVGSACTPGRWGRAWGGG*YK
RTAQQRDEDFGAQLCASPSRNLDHIRSSRAPAG
EALPRLAAPERLSRAPWQDPFSCPCRSYCYP*PW
KLQEKKPRVTRLLMAPHVQEAPTHGRWPCSVAI
SSTAEASWSMSAGCSLPPTAR*MSTPCTWAVIR
WATGELRGSRPRSHSATPATPHRPMLMTSCS*SS
IARPGCHPW*RKSGCPPAANPLEPPVLSPAGAL
PRAQM*PFPLTSCAWMSSSSPPRTARRFTRTYW
KIPCCALASPTPRKTPAMVTQGDRWCAEVPCKVW
CPGELSLAANPMTQESTLKCASSPSG*MTP*KS
IANATLS*LTVCFQQKMHRSEDADDL*SQI*LY
LSSKIYLNQPHALLINQSNW*RPKTKTNKETQNP
QCWRRVSETSTLKHWNWTFVQSLRKTLGQRTPR
PLFTTFDPVTLILGRTY*NKKNPKCRTRLEFTM
ILFITEMK*NHQTCSKSTRWLK**SGLAQRCFFS
LRQSLCCLGCNAVMQSWLTATSASWVQVILVLQ
PPKYLGLQVCTTTPG*FFVYFY*RQGFTMLASM
VLNA*PQMIHPPWPPKVLGLQA*ATTASPQ*YYK
PIKNDT*TELSVLFKNI*AIGC*MTKGRTKIYM
YVTLPIKNEIFTESDLKTHVPDCILGSS*GVSF
LLCTFP*MCTFIT*KIKVGKKV*R

Frame 2

ITRTANAGGGVPESAPPAPQGAGAGHGAGGDIR
GRPSRGMKILEPSCVPAQVGTWITSDPLELQQE
RPFLAWQPLSGSAGHHGKIPSPAPADLTAILSL
GNCRRRSPG*QDY*WRPMCKRLPPMAGGPAQWQS
APLRRRPGQ*ALGAHCRPLQDE*VHRAPGQ*YA
GRQESSEDQGLEVIPPPRLLHTDPC**PHAREA
Q*PGQAVIHGEESQAALPLRTPWNHLYCLRLGHY
HEPRCDLSL*PHVRGCQAHLPPGLHEGLQGLTG
KFHAVRWHPRLQEKRLQW*LRGTVGVQRYPARS
GVLGNFPLRPTQ*PRSLHSSVQVHQVDK*HHEKA
SLTPH*VN*LCASNRKCTGVRTPMTYEVKFDFT
FPQRYI*TNLMPC**TNQIGKDLKPKQIKKHKT
LSAGEESVRPALSNTGTGRSYSLYGRHLVNVHRD
PYSPPLTQ*L*S*EEPTETKKIQNVEQDLNLP*
YYLSQK*SETIKHVPKVPDGLNNSLAWHNDVFF
L*DRVSVAWAAMQ*CNLGSLQPPPPGFK*FSCFS
LPSTWDYRCAPPHQANFLCIFTRDRVSPCWPAW
S*TPDLR*STHLGLPKCWDYRHEPPRPAHNDIT
NLLKMILRQNCQYYSRTFRL*DVK*QKEGQKYIC
M*PYP*KMKYSQNQI*KHMSQTAYWGRHEVSPS
FCVLFLECALL*HEK*RWGKKSE

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