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Sequence OGG1.qAug05
ACATCGCCCGCATCACTGGCATGGTGGAGCGGCTGTGCCAGGCTTTTGGACCTCGGCTCATCCAGCTTGATGATGTCACCTACCATGGCTTCCCCAGCCTGCAGGCCCTGGCTGGAAACTTTTTCCGGAGCCTGTGGGGACCTTATGCTGGCTGGGCCCAAGCGGTGAGTGTACCTAGGTGTCCTCCCTAGGTTTCCTCT
CCTCCAGCCCAGACCCAGTGGACTCTTCCACCACCGCCCCAGGTGGCCCTAAAGGACTCTCCAGCCACCCCTGTCCCAACCCCAGTGGATTCTCATTGCC
TTCGGCCCTGTTCCCCAAGGACTCTTCCACCTCCCAACACTGTCACTAGTCTCACCAGCCCTGACCCCAGTGTACCCTCCTCCCCACACAGACTCCACCC
TCCTACAGGTGCTGTTCAGTGCCGACCTGCGCCAATCCCGCCATGCTCAGGAGCCACCAGCAAAGCGCAGAAAGGGTTCCAAAGGGCCGGAAGGCTAGAT
GGGGCACCCTGGACAAAGAAATTCCCCAAGCACCTTCCCCTCCATTCCCCACTTCTCTCTCCCCATCCCCACCCAGTCTCATGTTGGGGAGGGGCCTCCC
TGTGACTACCTCAAAGGCCAGGCACCCCCAAATCAAGCAGTCAGTTTGCACAACAAGATGGGGTGGGGGATATTGAGGGAGACAGCGCTAAGGATGGTTT
TATCTTCCCTTTATTACAAGAAGGAACAATAAAATAGAAACATTTGTATGGA
- OGG1.u.f.12 (forward primer)
- OGG1.u.r.14 (reverse primer)
- Single exon skipping
Frame 0
TSPASLAWWSGCARLLDLGSSSLMMSPTMASPACRPWLETFSGACGDLMLAGPKR*VYLGVLPRFPL
LQPRPSGLFHHRPRWP*RTLQPPLSQPQWILIA
FGPVPQGLFHLPTLSLVSPALTPVYPPPHTDST
LLQVLFSADLRQSRHAQEPPAKRRKGSKGPEG*M
GHPGQRNSPSTFPSIPHFSLPIPTQSHVGEGPP
CDYLKGQAPPNQAVSLHNKMGWGILRETALRMV
LSSLYYKKEQ*NRNICM
Frame 1
HRPHHWHGGAAVPGFWTSAHPA**CHLPWLPQPAGPGWKLFPEPVGTLCWLGPSGECT*VSSLGFL
SSSPDPVDSSTTAPGGPKGLSSHPCPNPSGFSLP
SALFPKDSSTSQHCH*SHQP*PQCTLLPTQTPP
SYRCCSVPTCANPAMLRSHQQSAERVPKGRKAR
WGTLDKEIPQAPSPPFPTSLSPSPPSLMLGRGLP
VTTSKARHPQIKQSVCTTRWGGGY*GRQR*GWF
YLPFITRRNNKIETFVW
Frame 2
IARITGMVERLCQAFGPRLIQLDDVTYHGFPSLQALAGNFFRSLWGPYAGWAQAVSVPRCPP*VSS
PPAQTQWTLPPPPQVALKDSPATPVPTPVDSHC
LRPCSPRTLPPPNTVTSLTSPDPSVPSSPHRLHP
PTGAVQCRPAPIPPCSGATSKAQKGFQRAGRLD
GAPWTKKFPKHLPLHSPLLSPHPHPVSCWGGAS
L*LPQRPGTPKSSSQFAQQDGVGDIEGDSAKDGF
IFPLLQEGTIK*KHLYG
Sequence OGG1.mAug05
ATCGCTTGAACCCGGGAGGCGGAGCTTGCAGTGAGCCGAGATCGCGCCATCACACTCCAGCCTAGGCGACAGAGTGAGACTCCGTCTCAAAGAAAAAAAACTTGCAGCCTGATAGTTAAGATACAGCAACCCCAAATCCCTATGCTAAAAGGTGAGAATGGCCCAGATAAAGGTCATGTCTCCTAGCTCCCTGCTTTTTC
ATGCCATCCTCCAGAAGGGAAGAAATTAAATAATCCATCCTCCTACTCCAGGCGACTAGAAGGCAGGCTGCCTCAGGGCCACACACTGGGACTTGGACTC
AACCTGATGGGCTTCTGGGCCCAGCCCCAGACAAACCCCCGGCAAACGTCCCATTCCGAGGAAAGCATGAGCAGATGGAGTATGGAAGAAATGCCCAAGA
CGGCAGGCAGCAGCTGTGGCGGCCGGCGGGACGACAATCCGAGGAGAGGCCTCTGATGTCCTGAGGTCTCAGAGGACGCCTAAAGGCCTTGAATGGGACA
AGCTTAGCGGGCGGGCGCAGAAGAGAATAATACTCTGGAGACACTTCCCGAGGGCTCTGGGGCCGGAGCTGTGTTCGCTCCGGTTCTTGGTGAAGACAGG
GTTCGTGGGAGGCGGCCCAAGGAGGGCGAACGCCTAAGACTGCAAAGGCTCGGGGGAGAACGGCTCTCGGAGAACGGGCTGGGGAAGGACGTGGCTCTGA
AGACGGACAGCCCTGAGGAACCGCGGGGCGCCCAGATGGAACTCGTTAGCGCCCCGAGTGCAGACAATCCCGGAGGGGGAAAGGCGAGCAGCTGGCAGAG
AGCCCAGTGCCGGCCAACCGCGCGAGCGCCTCAGAACGGCCCGCCCACCCTGATTTCTCATTGGCGCCTCCTACCTCCTCCTCGGATTGGCTACCTCTAG
GTGAAATGAGCGGTGGTTGAGCCCTACTTCCGGTGGTGCTGTGGTCTGCCCCTGGAGAACCCAGAAGAACACAGCTGTGCGCGCCCACAGGCTCTGGGGG
CGGGAGAAGATAAGTCGCAAGGAGGGGGCGGGACCTACACCTCAGGAAAGCCGGAGAATTGGGGCACGAAGCGGGGCTTTGATGACCCGCAAAGGGCGAG
GCATGCAGGAGGTGGAGGAATTAAGTGAAACAGGGAAGGTTGTTAAACAGCACCGTGTGGGCGAGGCCTTAAGGGTCGTGGTCCTTGTCTGGGCGGGGTC
TTTGGGCGTCGACGAGGCCTGGTTCTGGGTAGGCGGGGCTACTACGGGGCGGTGCCTGCTGTGGAAATGCCTGCCCGCGCGCTTCTGCCCAGGCGCATGG
GGCATCGTACTCTAGCCTCCACTCCTGCCCTGTGGGCCTCCATCCCGTGCCCTCGCTCTGAGCTGCGCCTGGACCTGGTTCTGCCTTCTGGACAATCTTT
CCGGTGGAGGGAGCAAAGTCCTGCACACTGGAGTGGTGTACTAGCGGATCAAGTATGGACACTGACTCAGACTGAGGAGCAGCTCCACTGCACTGTGTAC
CGAGGAGACAAGAGCCAGGCTAGCAGGCCCACACCAGACGAGCTGGAGGCCGTGCGCAAGTACTTCCAGCTAGATGTTACCCTGGCTCAACTGTATCACC
ACTGGGGTTCCGTGGACTCCCACTTCCAAGAGGTGGCTCAGAAATTCCAAGGTGTGCGACTGCTGCGACAAGACCCCATCGAATGCCTTTTCTCTTTTAT
CTGTTCCTCCAACAACAACATCGCCCGCATCACTGGCATGGTGGAGCGGCTGTGCCAGGCTTTTGGACCTCGGCTCATCCAGCTTGATGATGTCACCTAC
CATGGCTTCCCCAGCCTGCAGGCCCTGGCTGGTGGCTGACTGCATCTGCCTGATGGCCCTAGACAAGCCCCAGGCTGTGCCCGTGGATGTCCATATGTGG
CACATTGCCCAACGTGACTACAGCTGGCACCCTACCACGTCCCAGGCGAAGGGACCGAGCCCCCAGACCAACAAGGAACTGGGAAACTTTTTCCGGAGCC
TGTGGGGACCTTATGCTGGCTGGGCCCAAGCGGTGAGTGTACCTAGGTGTCCTCCCTAGGTTTCCTCTCCTCCAGCCCAGACCCAGTGGACTCTTCCACC
ACCGCCCCAGGTGGCCCTAAAGGACTCTCCAGCCACCCCTGTCCCAACCCCAGTGGATTCTCATTGCCTTCGGCCCTGTTCCCCAAGGACTCTTCCACCT
CCCAACACTGTCACTAGTCTCACCAGCCCTGACCCCAGTGTACCCTCCTCCCCACACAGACTCCACCCTCCTACAGGTGCTGTTCAGTGCCGACCTGCGC
CAATCCCGCCATGCTCAGGAGCCACCAGCAAAGCGCAGAAAGGGTTCCAAAGGGCCGGAAGGCTAGATGGGGCACCCTGGACAAAGAAATTCCCCAAGCA
CCTTCCCCTCCATTCCCCACTTCTCTCTCCCCATCCCCACCCAGTCTCATGTTGGGGAGGGGCCTCCCTGTGACTACCTCAAAGGCCAGGCACCCCCAAA
TCAAGCAGTCAGTTTGCACAACAAGATGGGGTGGGGGATATTGAGGGAGACAGCGCTAAGGATGGTTTTATCTTCCCTTTATTACAAGAAGGAACAATAA
AATAGAAACATTTGTATGGA
- OGG1.u.f.12 (forward primer)
- OGG1.u.r.14 (reverse primer)
- Single exon skipping
Frame 0
IA*TREAELAVSRDRAITLQPRRQSETPSQRKKTCSLIVKIQQPQIPMLKGENGPDKGHVS*LPAFS
CHPPEGKKLNNPSSYSRRLEGRLPQGHTLGLGL
NLMGFWAQPQTNPRQTSHSEESMSRWSMEEMPK
TAGSSCGGRRDDNPRRGL*CPEVSEDA*RP*MGQ
A*RAGAEENNTLETLPEGSGAGAVFAPVLGEDR
VRGRRPKEGERLRLQRLGGERLSENGLGKDVAL
KTDSPEEPRGAQMELVSAPSADNPGGGKASSWQR
AQCRPTARAPQNGPPTLISHWRLLPPPRIGYL*
VK*AVVEPYFRWCCGLPLENPEEHSCARPQALG
AGEDKSQGGGGTYTSGKPENWGTKRGFDDPQRAR
HAGGGGIK*NREGC*TAPCGRGLKGRGPCLGGV
FGRRRGLVLGRRGYYGAVPAVEMPARALLPRRM
GHRTLASTPALWASIPCPRSELRLDLVLPSGQSF
RWREQSPAHWSGVLADQVWTLTQTEEQLHCTVY
RGDKSQASRPTPDELEAVRKYFQLDVTLAQLYH
HWGSVDSHFQEVAQKFQGVRLLRQDPIECLFSFI
CSSNNNIARITGMVERLCQAFGPRLIQLDDVTY
HGFPSLQALAGG*LHLPDGPRQAPGCARGCPYV
AHCPT*LQLAPYHVPGEGTEPPDQQGTGKLFPEP
VGTLCWLGPSGECT*VSSLGFLSSSPDPVDSST
TAPGGPKGLSSHPCPNPSGFSLPSALFPKDSST
SQHCH*SHQP*PQCTLLPTQTPPSYRCCSVPTCA
NPAMLRSHQQSAERVPKGRKARWGTLDKEIPQA
PSPPFPTSLSPSPPSLMLGRGLPVTTSKARHPQ
IKQSVCTTRWGGGY*GRQR*GWFYLPFITRRNNK
IETFVW
Frame 1
SLEPGRRSLQ*AEIAPSHSSLGDRVRLRLKEKKLAA**LRYSNPKSLC*KVRMAQIKVMSPSSLLF
HAILQKGRN*IIHPPTPGD*KAGCLRATHWDLDS
T*WASGPSPRQTPGKRPIPRKA*ADGVWKKCPR
RQAAAVAAGGTTIRGEASDVLRSQRTPKGLEWD
KLSGRAQKRIILWRHFPRALGPELCSLRFLVKTG
FVGGGPRRANA*DCKGSGENGSRRTGWGRTWL*
RRTALRNRGAPRWNSLAPRVQTIPEGERRAAGR
EPSAGQPRERLRTARPP*FLIGASYLLLGLATSR
*NERWLSPTSGGAVVCPWRTQKNTAVRAHRLWG
REKISRKEGAGPTPQESRRIGARSGALMTRKGR
GMQEVEELSETGKVVKQHRVGEALRVVVLVWAGS
LGVDEAWFWVGGATTGRCLLWKCLPARFCPGAW
GIVL*PPLLPCGPPSRALALSCAWTWFCLLDNL
SGGGSKVLHTGVVY*RIKYGH*LRLRSSSTALCT
EETRARLAGPHQTSWRPCASTSS*MLPWLNCIT
TGVPWTPTSKRWLRNSKVCDCCDKTPSNAFSLL
SVPPTTTSPASLAWWSGCARLLDLGSSSLMMSPT
MASPACRPWLVADCICLMALDKPQAVPVDVHMW
HIAQRDYSWHPTTSQAKGPSPQTNKELGNFFRS
LWGPYAGWAQAVSVPRCPP*VSSPPAQTQWTLPP
PPQVALKDSPATPVPTPVDSHCLRPCSPRTLPP
PNTVTSLTSPDPSVPSSPHRLHPPTGAVQCRPA
PIPPCSGATSKAQKGFQRAGRLDGAPWTKKFPKH
LPLHSPLLSPHPHPVSCWGGASL*LPQRPGTPK
SSSQFAQQDGVGDIEGDSAKDGFIFPLLQEGTI
K*KHLYG
Frame 2
RLNPGGGACSEPRSRHHTPA*ATE*DSVSKKKNLQPDS*DTATPNPYAKR*EWPR*RSCLLAPCFF
MPSSRREEIK*SILLLQATRRQAASGPHTGTWT
QPDGLLGPAPDKPPANVPFRGKHEQMEYGRNAQD
GRQQLWRPAGRQSEERPLMS*GLRGRLKALNGT
SLAGGRRRE*YSGDTSRGLWGRSCVRSGSW*RQ
GSWEAAQGGRTPKTAKARGRTALGERAGEGRGSE
DGQP*GTAGRPDGTR*RPECRQSRRGKGEQLAE
SPVPANRASASERPAHPDFSLAPPTSSSDWLPL
GEMSGG*ALLPVVLWSAPGEPRRTQLCAPTGSGG
GRR*VARRGRDLHLRKAGELGHEAGL**PAKGE
ACRRWRN*VKQGRLLNSTVWARP*GSWSLSGRG
LWASTRPGSG*AGLLRGGACCGNACPRASAQAHG
ASYSSLHSCPVGLHPVPSL*AAPGPGSAFWTIF
PVEGAKSCTLEWCTSGSSMDTDSD*GAAPLHCV
PRRQEPG*QAHTRRAGGRAQVLPARCYPGSTVSP
LGFRGLPLPRGGSEIPRCATAATRPHRMPFLFY
LFLQQQHRPHHWHGGAAVPGFWTSAHPA**CHL
PWLPQPAGPGWWLTASA*WP*TSPRLCPWMSICG
TLPNVTTAGTLPRPRRRDRAPRPTRNWETFSGA
CGDLMLAGPKR*VYLGVLPRFPLLQPRPSGLFH
HRPRWP*RTLQPPLSQPQWILIAFGPVPQGLFHL
PTLSLVSPALTPVYPPPHTDSTLLQVLFSADLR
QSRHAQEPPAKRRKGSKGPEG*MGHPGQRNSPS
TFPSIPHFSLPIPTQSHVGEGPPCDYLKGQAPPN
QAVSLHNKMGWGILRETALRMVLSSLYYKKEQ*
NRNICM
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