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Sequences

Sequence BOK.fAug05

GCCCCAAGCCCAGCCTCTCGCCAGCTGGGAGTCGCGCGCTGCCCACCTCGCTGCCCAGGCCCCCGACGCCGCGGCAGGAGCCCCCCAAGAGCGCGGGAAG CCCCGTGGACCTGGCGCTCCCGGCTCGGGCGTGGACGGGGCGGGCGCCGGGGCGGGGCGCGCGTCCTCGCGGGTCTGAATGGAAGGGTCGAGGTCGTCGT CGGCGGCGAGCAGATCCTGAAGCCAGAACTCCACCCCGGCGCCCGCGCCATGCGGCGGGAGAGGCATCACGTGGGGCAAGGTGGTGTCCCTGTATGCGGT GGCCGCGGGGCTGGCCGTGGACTGTGTGAGGCAGGCCCAGCCTGCCATGGTCCACGCCCTCGTGGACTGCCTGGGGGAGTTCGTGCGCAAGACCCTGGCA ACCTGGCTGCGGAGACGCGGCGGATGGACTGATGTCCTCAAGTGTGTGGTCAGCACAGACCCTGGCCTCCGCTCCCACTGGCTGGTGGCTGCACTCTGCA GCTTCGGCCGCTTCCTGAAGGCTGCCTTCTTCGTGCTGCTGCCAGAGAGATGAGCTGCCCACCTGGCAGTGGCCGCAGCCTGGCCCTCTGGGCCCAACGC AGGAGGCCCTCAGCACCCGAACACATCTTCCTCCTCCCCACCCGAGCCTGGAGCACTCTAACCCTCGGAGACCCCCTAAGCCCCGTTCCTCCGCAGACCC AGGCCCTCCGGAAGGGGTGAGTGGGGAGGGGCTTTCCTGAGCCTGGAGCTGGGCTTTGGGGCAGCCTGCGACCCTCCCCGCTTGTGTCCCTTCTCCTGTG ATCTCTGTGTTTTCCCTTTTCTTTCTGGGGCCAGGAAGTCAGGGTCAACTCCCAGGCCTCAGATGCAGGGGCCCAGAACACCTGCTCTCACCTGAGCCCC AGGTGAAGGGGCCCGGGAACACCTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGGAACACCTGCTCTCACCTGAACCCCAGGTGAAGGGGCCCGGAA CACCTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGAACACCTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGGAACACCTGCTCTCACCTGAGC CCCAGGTGAAGGGGCCCAGAACACCTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGAACACCTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGG AACACCTGCTCTCACCTGAGCCCCTGGTGAAGGGGCCCGGAACACTTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGAACACCTGCTCTCACCTGAG CCCCCGGTGAAGGGGCCCGGAACACTTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGAACACCTCCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGG AACACCTCCTGTCACCTGAGCCCCAGGTGAAGGGGCCCGGGAACACCTCTCACCTGAACCCGGGGGTCCCATCCCAGGAAGAAGGGCCATCTCAGGACAT GAGTCCTCAGGGGCCCTGCACATTCAATCTGAAGGTGACCCTGGCCTGGCTGAAGCTGGAAGAGCTGTGGGGACTCAGCCTGTAAACAGAGCGTAAGGTT CACATGCTGGTTGCTTAATCCGTTTCTGGAGGAAGAGTATGACACCCACTTGTGATGGGGTCCTTGTGCGGTGGGGACCGGGGCCGGCGGGCTCCAGGCC AGCACACCTAACCCATGGATGTGGAACCTACGGCCGAGAAGGAATGTTGCATGAGTCGGATCCCAGTCCATTGTCAGTGGAGGGTGAGGGTGACCCCATC TGCTATTTTTGTGCTCATCCTCATACAACCATTTGGGGATGTGCCTATTAGGGCTCCGTAAGAACTCAGATGCCTGGGAAGCCCAGCCCCTCAGGTGCCC CCACACACAGCCTTCCCTTGACGCCTACATTTCTAGGCACATGTGAGGCATCTTTCCTGGAGCCCCGAGCCAGCCCTGTCCCTCCCCAGTGCAGCATGGC ACTCAGGAGATACAGGCTGGACATGGGGCAGTCGTTCTGGGGAGGCCTGGCCTAGCAGCCACCCACCTGAGCCCTCCCGGCCAGGCTTCGTGCTGGGGTG GGCCATGTGCCAGGACAGGAGGGTCCCGGCGGAAAGCCAGCCCCGGACTCATCGTGACATTGAGATCCCACTGGAGGGTAGGGGTGGTAATAAACTTCTC CAAACGATCGTTGTCATTTTAGACAGAA

BOK.F4 (forward primer)
BOK.R6 (reverse primer)
Multiple exon skipping

Frame 0

APSPASRQLGVARCPPRCPGPRRRGRSPPRARE APWTWRSRLGRGRGGRRGGARVLAGLNGRVEVVV GGEQILKPELHPGARAMRRERHHVGQGGVPVCG GRGAGRGLCEAGPACHGPRPRGLPGGVRAQDPG NLAAETRRMD*CPQVCGQHRPWPPLPLAGGCTLQ LRPLPEGCLLRAAAREMSCPPGSGRSLALWAQR RRPSAPEHIFLLPTRAWSTLTLGDPLSPVPPQT QALRKG*VGRGFPEPGAGLWGSLRPSPLVSLLL* SLCFPFSFWGQEVRVNSQASDAGAQNTCSHLSP R*RGPGTPALT*APGEGAREHLLSPEPQVKGPG TPALT*APGEGARNTCSHLSPR*RGPGTPALT*A PGEGAQNTCSHLSPR*RGPEHLLSPEPQVKGPG NTCSHLSPW*RGPEHLLSPEPQVKGPGTPALT* APGEGARNTCSHLSPR*RGPEHLLSPEPQVKGPG TPPVT*APGEGAREHLSPEPGGPIPGRRAISGH ESSGALHIQSEGDPGLAEAGRAVGTQPVNRA*G SHAGCLIRFWRKSMTPTCDGVLVRWGPGPAGSRP AHLTHGCGTYGREGMLHESDPSPLSVEGEGDPI CYFCAHPHTTIWGCAY*GSVRTQMPGKPSPSGA PTHSLPLTPTFLGTCEASFLEPRASPVPPQCSMA LRRYRLDMGQSFWGGLA*QPPT*ALPARLRAGV GHVPGQEGPGGKPAPDSS*H*DPTGG*GW**TS PNDRCHFRQ

Frame 1

PQAQPLASWESRAAHLAAQAPDAAAGAPQERGK PRGPGAPGSGVDGAGAGAGRASSRV*MEGSRSS SAASRS*SQNSTPAPAPCGGRGITWGKVVSLYAV AAGLAVDCVRQAQPAMVHALVDCLGEFVRKTLA TWLRRRGGWTDVLKCVVSTDPGLRSHWLVAALC SFGRFLKAAFFVLLPER*AAHLAVAAAWPSGPNA GGPQHPNTSSSSPPEPGAL*PSETP*APFLRRP RPSGRGEWGGAFLSLELGFGAACDPPRLCPFSC DLCVFPFLSGARKSGSTPRPQMQGPRTPALT*AP GEGAREHLLSPEPQVKGPGNTCSHLNPR*RGPE HLLSPEPQVKGPGTPALT*APGEGAREHLLSPE PQVKGPRTPALT*APGEGARNTCSHLSPR*RGPG TPALT*APGEGARNTCSHLSPR*RGPEHLLSPE PPVKGPGTLALT*APGEGARNTSSHLSPR*RGP EHLLSPEPQVKGPGNTSHLNPGVPSQEEGPSQDM SPQGPCTFNLKVTLAWLKLEELWGLSL*TERKV HMLVA*SVSGGRV*HPLVMGSLCGGDRGRRAPG QHT*PMDVEPTAEKECCMSRIPVHCQWRVRVTPS AIFVLILIQPFGDVPIRAP*ELRCLGSPAPQVP PHTAFP*RLHF*AHVRHLSWSPEPALSLPSAAW HSGDTGWTWGSRSGEAWPSSHPPEPSRPGFVLGW AMCQDRRVPAESQPRTHRDIEIPLEGRGGNKLL QTIVVILDR

Frame 2

PKPSLSPAGSRALPTSLPRPPTPRQEPPKSAGS PVDLALPARAWTGRAPGRGARPRGSEWKGRGRR RRRADPEARTPPRRPRHAAGEASRGARWCPCMR WPRGWPWTV*GRPSLPWSTPSWTAWGSSCARPWQ PGCGDAADGLMSSSVWSAQTLASAPTGWWLHSA ASAAS*RLPSSCCCQRDELPTWQWPQPGPLGPT QEALSTRTHLPPPHPSLEHSNPRRPPKPRSSADP GPPEGVSGEGLS*AWSWALGQPATLPACVPSPV ISVFSLFFLGPGSQGQLPGLRCRGPEHLLSPEP QVKGPGNTCSHLSPR*RGPGTPALT*TPGEGARN TCSHLSPR*RGPEHLLSPEPQVKGPGNTCSHLS PR*RGPEHLLSPEPQVKGPGTPALT*APGEGAR EHLLSPEPLVKGPGTLALT*APGEGARNTCSHLS PR*RGPEHLLSPEPQVKGPGTPPLT*APGEGAR NTSCHLSPR*RGPGTPLT*TRGSHPRKKGHLRT *VLRGPAHSI*R*PWPG*SWKSCGDSACKQSVRF TCWLLNPFLEEEYDTHL*WGPCAVGTGAGGLQA STPNPWMWNLRPRRNVA*VGSQSIVSGG*G*PH LLFLCSSSYNHLGMCLLGLRKNSDAWEAQPLRCP HTQPSLDAYISRHM*GIFPGAPSQPCPSPVQHG TQEIQAGHGAVVLGRPGLAATHLSPPGQASCWG GPCARTGGSRRKASPGLIVTLRSHWRVGVVINFS KRSLSF*TE

Sequence BOK.bAug05

GCCCCAAGCCCAGCCTCTCGCCAGCTGGGAGTCGCGCGCTGCCCACCTCGCTGCCCAGGCCCCCGACGCCGCGGCAGGAGCCCCCCAAGAGCGCGGGAAG CCCCGTGGACCTGGCGCTCCCGGCTCGGGCGTGGACGGGGCGGGCGCCGGGGCGGGGCGCGCGTCCTCGCGGGTCTGAATGGAAGGGTCGAGGTCGTCGT CGGCGGCGAGCAGATCCTGAAGCCAGAACTCCACCCCGGCGCCCGCGCCATGCGGCGGGAGAGGTGCGGCGCCCCCCACCCGCGTCGCCGCCATGGAGGT GCTGCGGCGCTCCTCGGTCTTCGCCGCCGAGATCATGGACGCCTTTGACCGCTCGCCCACAGACAAGGAGCTGGTGGCCCAGGCCAAGGCGCTGGGCCGG GAGTACGTGCACGCGCGGCTGCTGCGCGCCGGCCTCTCCTGGAGCGCGCCCGAGCGTGCCGCGCCGGTCCCGGGACGCCTGGCTGAGGTGTGCGCGGTGC TGCTGCGCCTGGGCGATGAGCTGGAGATGATCCGGCCCAGCGTCTACCGCAACGTGGCGCGTCAGCTGCACATCTCCCTGCAGTCTGAGCCTGTGGTGAC CGATGCGTTCCTGGCCGTGGCTGGCCACATCTTCTCTGCAGGCATCACGTGGGGCAAGGTGGTGTCCCTGTATGCGGTGGCCGCGGGGCTGGCCGTGGAC TGTGTGAGGCAGGCCCAGCCTGCCATGGTCCACGCCCTCGTGGACTGCCTGGGGGAGTTCGTGCGCAAGACCCTGGCAACCTGGCTGCGGAGACGCGGCG GATGGACTGATGTCCTCAAGTGTGTGGTCAGCACAGACCCTGGCCTCCGCTCCCACTGGCTGGTGGCTGCACTCTGCAGCTTCGGCCGCTTCCTGAAGGC TGCCTTCTTCGTGCTGCTGCCAGAGAGATGAGCTGCCCACCTGGCAGTGGCCGCAGCCTGGCCCTCTGGGCCCAACGCAGGAGGCCCTCAGCACCCGAAC ACATCTTCCTCCGCAGACCCAGGCCCTCCGGAAGGGGTGAGTGGGGAGGGGCTTTCCTGAGCCTGGAGCTGGGCTTTGGGGCAGCCTGCGACCCTCCCCG CTTGTGTCCCTTCTCCTGTGATCTCTGTGTTTTCCCTTTTCTTTCTGGGGCCAGGAAGTCAGGGTCAACTCCCAGGCCTCAGATGCAGGGGCCCAGAACA CCTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGGAACACCTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGGAACACCTGCTCTCACCTGAACC CCAGGTGAAGGGGCCCGGAACACCTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGGAACACCTGCTCTCACCTGAACCCCAGGTGAAGGGGCCCAGA ACACCTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGAACACCTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGGAACACCTGCTCTCACCTGAG CCCCTGGTGAAGGGGCCCGGAACACTTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGAACACCTGCTCTCACCTGAGCCCCCGGTGAAGGGGCCCGG AACACTTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGAACACCTCCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGAACACCTCCTGTCACCTGAG CCCCAGGTGAAGGGGCCCGGGAACACCTCTCACCTGAACCCGGGGGTCCCATCCCAGGAAGAAGGGCCATCTCAGGACATGAGTCCTCAGGGGCCCTGCA CATTCAATCTGAAGGTGACCCTGGCCTGGCTGAAGCTGGAAGAGCTGTGGGGACTCAGCCTGTAAACAGAGCGTAAGGTTCACATGCTGGTTGCTTAATC CGTTTCTGGAGGAAGAGTATGACACCCACTTGTGATGGGGTCCTTGTGCGGTGGGGACCGGGGCCGGCGGGCTCCAGGCCAGCACACCTAACCCATGGAT GTGGAACCTACGGCCGAGAAGGAATGTTGCATGAGTCGGATCCCAGTCCATTGTCAGTGGAGGGTGAGGGTGACCCCATCTGCTATTTTTGTGCTCATCC TCATACAACCATTTGGGGATGTGCCTATTAGGGCTCCGTAAGAACTCAGATGCCTGGGAAGCCCAGCCCCTCAGGTGCCCCCACACACAGCCTTCCCTTG ACGCCTACATTTCTAGGCACATGTGAGGCATCTTTCCTGGAGCCCCGAGCCAGCCCTGTCCCTCCCCAGTGCAGCATGGCACTCAGGAGATACAGGCTGG ACATGGGGCAGTCGTTCTGGGGAGGCCTGGCCTAGCAGCCACCCACCTGAGCCCTCCCGGCCAGGCTTCGTGCTGGGGTGGGCCATGTGCCAGGACAGGA GGGTCCCGGCGGAAAGCCAGCCCCGGACTCATCGTGACATTGAGATCCCACTGGAGGGTAGGGGTGGTAATAAACTTCTCCAAACGATCGTTGTCATTTT AGACAGAA

BOK.F4 (forward primer)
BOK.R6 (reverse primer)
Multiple exon skipping

Frame 0

APSPASRQLGVARCPPRCPGPRRRGRSPPRARE APWTWRSRLGRGRGGRRGGARVLAGLNGRVEVVV GGEQILKPELHPGARAMRRERCGAPHPRRRHGG AAALLGLRRRDHGRL*PLAHRQGAGGPGQGAGP GVRARAAAARRPLLERARACRAGPGTPG*GVRGA AAPGR*AGDDPAQRLPQRGASAAHLPAV*ACGD RCVPGRGWPHLLCRHHVGQGGVPVCGGRGAGRG LCEAGPACHGPRPRGLPGGVRAQDPGNLAAETRR MD*CPQVCGQHRPWPPLPLAGGCTLQLRPLPEG CLLRAAAREMSCPPGSGRSLALWAQRRRPSAPE HIFLRRPRPSGRGEWGGAFLSLELGFGAACDPPR LCPFSCDLCVFPFLSGARKSGSTPRPQMQGPRT PALT*APGEGAREHLLSPEPQVKGPGNTCSHLN PR*RGPEHLLSPEPQVKGPGNTCSHLNPR*RGPE HLLSPEPQVKGPGTPALT*APGEGAREHLLSPE PLVKGPGTLALT*APGEGARNTCSHLSPR*RGP EHLLSPEPQVKGPGTPPLT*APGEGARNTSCHLS PR*RGPGTPLT*TRGSHPRKKGHLRT*VLRGPA HSI*R*PWPG*SWKSCGDSACKQSVRFTCWLLN PFLEEEYDTHL*WGPCAVGTGAGGLQASTPNPWM WNLRPRRNVA*VGSQSIVSGG*G*PHLLFLCSS SYNHLGMCLLGLRKNSDAWEAQPLRCPHTQPSL DAYISRHM*GIFPGAPSQPCPSPVQHGTQEIQAG HGAVVLGRPGLAATHLSPPGQASCWGGPCARTG GSRRKASPGLIVTLRSHWRVGVVINFSKRSLSF *TE

Frame 1

PQAQPLASWESRAAHLAAQAPDAAAGAPQERGK PRGPGAPGSGVDGAGAGAGRASSRV*MEGSRSS SAASRS*SQNSTPAPAPCGGRGAAPPTRVAAMEV LRRSSVFAAEIMDAFDRSPTDKELVAQAKALGR EYVHARLLRAGLSWSAPERAAPVPGRLAEVCAV LLRLGDELEMIRPSVYRNVARQLHISLQSEPVVT DAFLAVAGHIFSAGITWGKVVSLYAVAAGLAVD CVRQAQPAMVHALVDCLGEFVRKTLATWLRRRG GWTDVLKCVVSTDPGLRSHWLVAALCSFGRFLKA AFFVLLPER*AAHLAVAAAWPSGPNAGGPQHPN TSSSADPGPPEGVSGEGLS*AWSWALGQPATLP ACVPSPVISVFSLFFLGPGSQGQLPGLRCRGPEH LLSPEPQVKGPGNTCSHLSPR*RGPGTPALT*T PGEGARNTCSHLSPR*RGPGTPALT*TPGEGAQ NTCSHLSPR*RGPEHLLSPEPQVKGPGNTCSHLS PW*RGPEHLLSPEPQVKGPGTPALT*APGEGAR NTCSHLSPR*RGPEHLLSPEPQVKGPGTPPVT* APGEGAREHLSPEPGGPIPGRRAISGHESSGALH IQSEGDPGLAEAGRAVGTQPVNRA*GSHAGCLI RFWRKSMTPTCDGVLVRWGPGPAGSRPAHLTHG CGTYGREGMLHESDPSPLSVEGEGDPICYFCAHP HTTIWGCAY*GSVRTQMPGKPSPSGAPTHSLPL TPTFLGTCEASFLEPRASPVPPQCSMALRRYRL DMGQSFWGGLA*QPPT*ALPARLRAGVGHVPGQE GPGGKPAPDSS*H*DPTGG*GW**TSPNDRCHF RQ

Frame 2

PKPSLSPAGSRALPTSLPRPPTPRQEPPKSAGS PVDLALPARAWTGRAPGRGARPRGSEWKGRGRR RRRADPEARTPPRRPRHAAGEVRRPPPASPPWR CCGAPRSSPPRSWTPLTARPQTRSWWPRPRRWAG STCTRGCCAPASPGARPSVPRRSRDAWLRCARC CCAWAMSWR*SGPASTATWRVSCTSPCSLSLW* PMRSWPWLATSSLQASRGARWCPCMRWPRGWPWT V*GRPSLPWSTPSWTAWGSSCARPWQPGCGDAA DGLMSSSVWSAQTLASAPTGWWLHSAASAAS*R LPSSCCCQRDELPTWQWPQPGPLGPTQEALSTRT HLPPQTQALRKG*VGRGFPEPGAGLWGSLRPSP LVSLLL*SLCFPFSFWGQEVRVNSQASDAGAQN TCSHLSPR*RGPGTPALT*APGEGAREHLLSPEP QVKGPGTPALT*APGEGAREHLLSPEPQVKGPR TPALT*APGEGARNTCSHLSPR*RGPGTPALT* APGEGARNTCSHLSPR*RGPEHLLSPEPPVKGPG TLALT*APGEGARNTSSHLSPR*RGPEHLLSPE PQVKGPGNTSHLNPGVPSQEEGPSQDMSPQGPC TFNLKVTLAWLKLEELWGLSL*TERKVHMLVA*S VSGGRV*HPLVMGSLCGGDRGRRAPGQHT*PMD VEPTAEKECCMSRIPVHCQWRVRVTPSAIFVLI LIQPFGDVPIRAP*ELRCLGSPAPQVPPHTAFP* RLHF*AHVRHLSWSPEPALSLPSAAWHSGDTGW TWGSRSGEAWPSSHPPEPSRPGFVLGWAMCQDR RVPAESQPRTHRDIEIPLEGRGGNKLLQTIVVIL DR

Sequence BOK.aAug05

GCCCCAAGCCCAGCCTCTCGCCAGCTGGGAGTCGCGCGCTGCCCACCTCGCTGCCCAGGCCCCCGACGCCGCGGCAGGAGCCCCCCAAGAGCGCGGGAAG CCCCGTGGACCTGGCGCTCCCGGCTCGGGCGTGGACGGGGCGGGCGCCGGGGCGGGGCGCGCGTCCTCGCGGGTCTGAATGGAAGGGTCGAGGTCGTCGT CGGCGGCGAGCAGATCCTGAAGCCAGAACTCCACCCCGGCGCCCGCGCCATGCGGCGGGAGAGGTGCGGCGCCCCCCACCCGCGTCGCCGCCATGGAGGT GCTGCGGCGCTCCTCGGTCTTCGCCGCCGAGATCATGGACGCCTTTGACCGCTCGCCCACAGACAAGGAGCTGGTGGCCCAGGCCAAGGCGCTGGGCCGG GAGTACGTGCACGCGCGGCTGCTGCGCGCCGGCCTCTCCTGGAGCGCGCCCGAGCGTGCCGCGCCGGTCCCGGGACGCCTGGCTGAGGTGTGCGCGGTGC TGCTGCGCCTGGGCGATGAGCTGGAGATGATCCGGCCCAGCGTCTACCGCAACGTGGCGCGTCAGCTGCACATCTCCCTGCAGTCTGAGCCTGTGGTGAC CGATGCGTTCCTGGCCGTGGCTGGCCACATCTTCTCTGCAGGCATCACGTGGGGCAAGGTGGTGTCCCTGTATGCGGTGGCCGCGGGGCTGGCCGTGGAC TGTGTGAGGCAGGCCCAGCCTGCCATGGTCCACGCCCTCGTGGACTGCCTGGGGGAGTTCGTGCGCAAGACCCTGGCAACCTGGCTGCGGAGACGCGGCG GATGGACTGATGTCCTCAAGTGTGTGGTCAGCACAGACCCTGGCCTCCGCTCCCACTGGCTGGTGGCTGCACTCTGCAGCTTCGGCCGCTTCCTGAAGGC TGCCTTCTTCGTGCTGCTGCCAGAGAGATGAGCTGCCCACCTGGCAGTGGCCGCAGCCTGGCCCTCTGGGCCCAACGCAGGAGGCCCTCAGCACCCGAAC ACATCTTCCTCCTCCCCACCCGAGCCTGGAGCACTCTAACCCTCGGAGACCCCCTAAGCCCCGTTCCTCCGCAGACCCAGGCCCTCCGGAAGGGGTGAGT GGGGAGGGGCTTTCCTGAGCCTGGAGCTGGGCTTTGGGGCAGCCTGCGACCCTCCCCGCTTGTGTCCCTTCTCCTGTGATCTCTGTGTTTTCCCTTTTCT TTCTGGGGCCAGGAAGTCAGGGTCAACTCCCAGGCCTCAGATGCAGGGGCCCAGAACACCTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGGAACAC CTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGGAACACCTGCTCTCACCTGAACCCCAGGTGAAGGGGCCCGGAACACCTGCTCTCACCTGAGCCCC AGGTGAAGGGGCCCGGAACACCTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGGAACACCTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGGAA CACCTGCTCTCACCTGAACCCCAGGTGAAGGGGCCCAGAACACCTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGAACACCTGCTCTCACCTGAGCC CCAGGTGAAGGGGCCCGGGAACACCTGCTCTCACCTGAGCCCCTGGTGAAGGGGCCCGGAACACTTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGA ACACCTGCTCTCACCTGAGCCCCCGGTGAAGGGGCCCGGAACACTTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGAACACCTCCTCTCACCTGAGC CCCAGGTGAAGGGGCCCGGAACACCTCCTGTCACCTGAGCCCCAGGTGAAGGGGCCCGGGAACACCTCTCACCTGAACCCGGGGGTCCCATCCCAGGAAG AAGGGCCATCTCAGGACATGAGTCCTCAGGGGCCCTGCACATTCAATCTGAAGGTGACCCTGGCCTGGCTGAAGCTGGAAGAGCTGTGGGGACTCAGCCT GTAAACAGAGCGTAAGGTTCACATGCTGGTTGCTTAATCCGTTTCTGGAGGAAGAGTATGACACCCACTTGTGATGGGGTCCTTGTGCGGTGGGGACCGG GGCCGGCGGGCTCCAGGCCAGCACACCTAACCCATGGATGTGGAACCTACGGCCGAGAAGGAATGTTGCATGAGTCGGATCCCAGTCCATTGTCAGTGGA GGGTGAGGGTGACCCCATCTGCTATTTTTGTGCTCATCCTCATACAACCATTTGGGGATGTGCCTATTAGGGCTCCGTAAGAACTCAGATGCCTGGGAAG CCCAGCCCCTCAGGTGCCCCCACACACAGCCTTCCCTTGACGCCTACATTTCTAGGCACATGTGAGGCATCTTTCCTGGAGCCCCGAGCCAGCCCTGTCC CTCCCCAGTGCAGCATGGCACTCAGGAGATACAGGCTGGACATGGGGCAGTCGTTCTGGGGAGGCCTGGCCTAGCAGCCACCCACCTGAGCCCTCCCGGC CAGGCTTCGTGCTGGGGTGGGCCATGTGCCAGGACAGGAGGGTCCCGGCGGAAAGCCAGCCCCGGACTCATCGTGACATTGAGATCCCACTGGAGGGTAG GGGTGGTAATAAACTTCTCCAAACGATCGTTGTCATTTTAGACAG

BOK.F4 (forward primer)
BOK.R6 (reverse primer)
Multiple exon skipping

Frame 0

APSPASRQLGVARCPPRCPGPRRRGRSPPRARE APWTWRSRLGRGRGGRRGGARVLAGLNGRVEVVV GGEQILKPELHPGARAMRRERCGAPHPRRRHGG AAALLGLRRRDHGRL*PLAHRQGAGGPGQGAGP GVRARAAAARRPLLERARACRAGPGTPG*GVRGA AAPGR*AGDDPAQRLPQRGASAAHLPAV*ACGD RCVPGRGWPHLLCRHHVGQGGVPVCGGRGAGRG LCEAGPACHGPRPRGLPGGVRAQDPGNLAAETRR MD*CPQVCGQHRPWPPLPLAGGCTLQLRPLPEG CLLRAAAREMSCPPGSGRSLALWAQRRRPSAPE HIFLLPTRAWSTLTLGDPLSPVPPQTQALRKG*V GRGFPEPGAGLWGSLRPSPLVSLLL*SLCFPFS FWGQEVRVNSQASDAGAQNTCSHLSPR*RGPGT PALT*APGEGAREHLLSPEPQVKGPGTPALT*AP GEGARNTCSHLSPR*RGPGTPALT*APGEGARE HLLSPEPQVKGPRTPALT*APGEGARNTCSHLS PR*RGPGTPALT*APGEGARNTCSHLSPR*RGPE HLLSPEPPVKGPGTLALT*APGEGARNTSSHLS PR*RGPEHLLSPEPQVKGPGNTSHLNPGVPSQE EGPSQDMSPQGPCTFNLKVTLAWLKLEELWGLSL *TERKVHMLVA*SVSGGRV*HPLVMGSLCGGDR GRRAPGQHT*PMDVEPTAEKECCMSRIPVHCQW RVRVTPSAIFVLILIQPFGDVPIRAP*ELRCLGS PAPQVPPHTAFP*RLHF*AHVRHLSWSPEPALS LPSAAWHSGDTGWTWGSRSGEAWPSSHPPEPSR PGFVLGWAMCQDRRVPAESQPRTHRDIEIPLEGR GGNKLLQTIVVILD

Frame 1

PQAQPLASWESRAAHLAAQAPDAAAGAPQERGK PRGPGAPGSGVDGAGAGAGRASSRV*MEGSRSS SAASRS*SQNSTPAPAPCGGRGAAPPTRVAAMEV LRRSSVFAAEIMDAFDRSPTDKELVAQAKALGR EYVHARLLRAGLSWSAPERAAPVPGRLAEVCAV LLRLGDELEMIRPSVYRNVARQLHISLQSEPVVT DAFLAVAGHIFSAGITWGKVVSLYAVAAGLAVD CVRQAQPAMVHALVDCLGEFVRKTLATWLRRRG GWTDVLKCVVSTDPGLRSHWLVAALCSFGRFLKA AFFVLLPER*AAHLAVAAAWPSGPNAGGPQHPN TSSSSPPEPGAL*PSETP*APFLRRPRPSGRGE WGGAFLSLELGFGAACDPPRLCPFSCDLCVFPFL SGARKSGSTPRPQMQGPRTPALT*APGEGAREH LLSPEPQVKGPGNTCSHLNPR*RGPEHLLSPEP QVKGPGTPALT*APGEGAREHLLSPEPQVKGPGN TCSHLNPR*RGPEHLLSPEPQVKGPGTPALT*A PGEGAREHLLSPEPLVKGPGTLALT*APGEGAR NTCSHLSPR*RGPEHLLSPEPQVKGPGTPPLT*A PGEGARNTSCHLSPR*RGPGTPLT*TRGSHPRK KGHLRT*VLRGPAHSI*R*PWPG*SWKSCGDSA CKQSVRFTCWLLNPFLEEEYDTHL*WGPCAVGTG AGGLQASTPNPWMWNLRPRRNVA*VGSQSIVSG G*G*PHLLFLCSSSYNHLGMCLLGLRKNSDAWE AQPLRCPHTQPSLDAYISRHM*GIFPGAPSQPCP SPVQHGTQEIQAGHGAVVLGRPGLAATHLSPPG QASCWGGPCARTGGSRRKASPGLIVTLRSHWRV GVVINFSKRSLSF*T

Frame 2

PKPSLSPAGSRALPTSLPRPPTPRQEPPKSAGS PVDLALPARAWTGRAPGRGARPRGSEWKGRGRR RRRADPEARTPPRRPRHAAGEVRRPPPASPPWR CCGAPRSSPPRSWTPLTARPQTRSWWPRPRRWAG STCTRGCCAPASPGARPSVPRRSRDAWLRCARC CCAWAMSWR*SGPASTATWRVSCTSPCSLSLW* PMRSWPWLATSSLQASRGARWCPCMRWPRGWPWT V*GRPSLPWSTPSWTAWGSSCARPWQPGCGDAA DGLMSSSVWSAQTLASAPTGWWLHSAASAAS*R LPSSCCCQRDELPTWQWPQPGPLGPTQEALSTRT HLPPPHPSLEHSNPRRPPKPRSSADPGPPEGVS GEGLS*AWSWALGQPATLPACVPSPVISVFSLF FLGPGSQGQLPGLRCRGPEHLLSPEPQVKGPGNT CSHLSPR*RGPGTPALT*TPGEGARNTCSHLSP R*RGPEHLLSPEPQVKGPGNTCSHLSPR*RGPG TPALT*TPGEGAQNTCSHLSPR*RGPEHLLSPEP QVKGPGNTCSHLSPW*RGPEHLLSPEPQVKGPG TPALT*APGEGARNTCSHLSPR*RGPEHLLSPE PQVKGPGTPPVT*APGEGAREHLSPEPGGPIPGR RAISGHESSGALHIQSEGDPGLAEAGRAVGTQP VNRA*GSHAGCLIRFWRKSMTPTCDGVLVRWGP GPAGSRPAHLTHGCGTYGREGMLHESDPSPLSVE GEGDPICYFCAHPHTTIWGCAY*GSVRTQMPGK PSPSGAPTHSLPLTPTFLGTCEASFLEPRASPV PPQCSMALRRYRLDMGQSFWGGLA*QPPT*ALPA RLRAGVGHVPGQEGPGGKPAPDSS*H*DPTGG* GW**TSPNDRCHFRQ