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Sequences

Sequence BOK.bAug05

GCCCCAAGCCCAGCCTCTCGCCAGCTGGGAGTCGCGCGCTGCCCACCTCGCTGCCCAGGCCCCCGACGCCGCGGCAGGAGCCCCCCAAGAGCGCGGGAAG CCCCGTGGACCTGGCGCTCCCGGCTCGGGCGTGGACGGGGCGGGCGCCGGGGCGGGGCGCGCGTCCTCGCGGGTCTGAATGGAAGGGTCGAGGTCGTCGT CGGCGGCGAGCAGATCCTGAAGCCAGAACTCCACCCCGGCGCCCGCGCCATGCGGCGGGAGAGGTGCGGCGCCCCCCACCCGCGTCGCCGCCATGGAGGT GCTGCGGCGCTCCTCGGTCTTCGCCGCCGAGATCATGGACGCCTTTGACCGCTCGCCCACAGACAAGGAGCTGGTGGCCCAGGCCAAGGCGCTGGGCCGG GAGTACGTGCACGCGCGGCTGCTGCGCGCCGGCCTCTCCTGGAGCGCGCCCGAGCGTGCCGCGCCGGTCCCGGGACGCCTGGCTGAGGTGTGCGCGGTGC TGCTGCGCCTGGGCGATGAGCTGGAGATGATCCGGCCCAGCGTCTACCGCAACGTGGCGCGTCAGCTGCACATCTCCCTGCAGTCTGAGCCTGTGGTGAC CGATGCGTTCCTGGCCGTGGCTGGCCACATCTTCTCTGCAGGCATCACGTGGGGCAAGGTGGTGTCCCTGTATGCGGTGGCCGCGGGGCTGGCCGTGGAC TGTGTGAGGCAGGCCCAGCCTGCCATGGTCCACGCCCTCGTGGACTGCCTGGGGGAGTTCGTGCGCAAGACCCTGGCAACCTGGCTGCGGAGACGCGGCG GATGGACTGATGTCCTCAAGTGTGTGGTCAGCACAGACCCTGGCCTCCGCTCCCACTGGCTGGTGGCTGCACTCTGCAGCTTCGGCCGCTTCCTGAAGGC TGCCTTCTTCGTGCTGCTGCCAGAGAGATGAGCTGCCCACCTGGCAGTGGCCGCAGCCTGGCCCTCTGGGCCCAACGCAGGAGGCCCTCAGCACCCGAAC ACATCTTCCTCCGCAGACCCAGGCCCTCCGGAAGGGGTGAGTGGGGAGGGGCTTTCCTGAGCCTGGAGCTGGGCTTTGGGGCAGCCTGCGACCCTCCCCG CTTGTGTCCCTTCTCCTGTGATCTCTGTGTTTTCCCTTTTCTTTCTGGGGCCAGGAAGTCAGGGTCAACTCCCAGGCCTCAGATGCAGGGGCCCAGAACA CCTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGGAACACCTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGGAACACCTGCTCTCACCTGAACC CCAGGTGAAGGGGCCCGGAACACCTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGGAACACCTGCTCTCACCTGAACCCCAGGTGAAGGGGCCCAGA ACACCTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGAACACCTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGGAACACCTGCTCTCACCTGAG CCCCTGGTGAAGGGGCCCGGAACACTTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGAACACCTGCTCTCACCTGAGCCCCCGGTGAAGGGGCCCGG AACACTTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGAACACCTCCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGAACACCTCCTGTCACCTGAG CCCCAGGTGAAGGGGCCCGGGAACACCTCTCACCTGAACCCGGGGGTCCCATCCCAGGAAGAAGGGCCATCTCAGGACATGAGTCCTCAGGGGCCCTGCA CATTCAATCTGAAGGTGACCCTGGCCTGGCTGAAGCTGGAAGAGCTGTGGGGACTCAGCCTGTAAACAGAGCGTAAGGTTCACATGCTGGTTGCTTAATC CGTTTCTGGAGGAAGAGTATGACACCCACTTGTGATGGGGTCCTTGTGCGGTGGGGACCGGGGCCGGCGGGCTCCAGGCCAGCACACCTAACCCATGGAT GTGGAACCTACGGCCGAGAAGGAATGTTGCATGAGTCGGATCCCAGTCCATTGTCAGTGGAGGGTGAGGGTGACCCCATCTGCTATTTTTGTGCTCATCC TCATACAACCATTTGGGGATGTGCCTATTAGGGCTCCGTAAGAACTCAGATGCCTGGGAAGCCCAGCCCCTCAGGTGCCCCCACACACAGCCTTCCCTTG ACGCCTACATTTCTAGGCACATGTGAGGCATCTTTCCTGGAGCCCCGAGCCAGCCCTGTCCCTCCCCAGTGCAGCATGGCACTCAGGAGATACAGGCTGG ACATGGGGCAGTCGTTCTGGGGAGGCCTGGCCTAGCAGCCACCCACCTGAGCCCTCCCGGCCAGGCTTCGTGCTGGGGTGGGCCATGTGCCAGGACAGGA GGGTCCCGGCGGAAAGCCAGCCCCGGACTCATCGTGACATTGAGATCCCACTGGAGGGTAGGGGTGGTAATAAACTTCTCCAAACGATCGTTGTCATTTT AGACAGAA

BOK.F4 (forward primer)
BOK.R6 (reverse primer)
Single exon skipping

Frame 0

APSPASRQLGVARCPPRCPGPRRRGRSPPRARE APWTWRSRLGRGRGGRRGGARVLAGLNGRVEVVV GGEQILKPELHPGARAMRRERCGAPHPRRRHGG AAALLGLRRRDHGRL*PLAHRQGAGGPGQGAGP GVRARAAAARRPLLERARACRAGPGTPG*GVRGA AAPGR*AGDDPAQRLPQRGASAAHLPAV*ACGD RCVPGRGWPHLLCRHHVGQGGVPVCGGRGAGRG LCEAGPACHGPRPRGLPGGVRAQDPGNLAAETRR MD*CPQVCGQHRPWPPLPLAGGCTLQLRPLPEG CLLRAAAREMSCPPGSGRSLALWAQRRRPSAPE HIFLRRPRPSGRGEWGGAFLSLELGFGAACDPPR LCPFSCDLCVFPFLSGARKSGSTPRPQMQGPRT PALT*APGEGAREHLLSPEPQVKGPGNTCSHLN PR*RGPEHLLSPEPQVKGPGNTCSHLNPR*RGPE HLLSPEPQVKGPGTPALT*APGEGAREHLLSPE PLVKGPGTLALT*APGEGARNTCSHLSPR*RGP EHLLSPEPQVKGPGTPPLT*APGEGARNTSCHLS PR*RGPGTPLT*TRGSHPRKKGHLRT*VLRGPA HSI*R*PWPG*SWKSCGDSACKQSVRFTCWLLN PFLEEEYDTHL*WGPCAVGTGAGGLQASTPNPWM WNLRPRRNVA*VGSQSIVSGG*G*PHLLFLCSS SYNHLGMCLLGLRKNSDAWEAQPLRCPHTQPSL DAYISRHM*GIFPGAPSQPCPSPVQHGTQEIQAG HGAVVLGRPGLAATHLSPPGQASCWGGPCARTG GSRRKASPGLIVTLRSHWRVGVVINFSKRSLSF *TE

Frame 1

PQAQPLASWESRAAHLAAQAPDAAAGAPQERGK PRGPGAPGSGVDGAGAGAGRASSRV*MEGSRSS SAASRS*SQNSTPAPAPCGGRGAAPPTRVAAMEV LRRSSVFAAEIMDAFDRSPTDKELVAQAKALGR EYVHARLLRAGLSWSAPERAAPVPGRLAEVCAV LLRLGDELEMIRPSVYRNVARQLHISLQSEPVVT DAFLAVAGHIFSAGITWGKVVSLYAVAAGLAVD CVRQAQPAMVHALVDCLGEFVRKTLATWLRRRG GWTDVLKCVVSTDPGLRSHWLVAALCSFGRFLKA AFFVLLPER*AAHLAVAAAWPSGPNAGGPQHPN TSSSADPGPPEGVSGEGLS*AWSWALGQPATLP ACVPSPVISVFSLFFLGPGSQGQLPGLRCRGPEH LLSPEPQVKGPGNTCSHLSPR*RGPGTPALT*T PGEGARNTCSHLSPR*RGPGTPALT*TPGEGAQ NTCSHLSPR*RGPEHLLSPEPQVKGPGNTCSHLS PW*RGPEHLLSPEPQVKGPGTPALT*APGEGAR NTCSHLSPR*RGPEHLLSPEPQVKGPGTPPVT* APGEGAREHLSPEPGGPIPGRRAISGHESSGALH IQSEGDPGLAEAGRAVGTQPVNRA*GSHAGCLI RFWRKSMTPTCDGVLVRWGPGPAGSRPAHLTHG CGTYGREGMLHESDPSPLSVEGEGDPICYFCAHP HTTIWGCAY*GSVRTQMPGKPSPSGAPTHSLPL TPTFLGTCEASFLEPRASPVPPQCSMALRRYRL DMGQSFWGGLA*QPPT*ALPARLRAGVGHVPGQE GPGGKPAPDSS*H*DPTGG*GW**TSPNDRCHF RQ

Frame 2

PKPSLSPAGSRALPTSLPRPPTPRQEPPKSAGS PVDLALPARAWTGRAPGRGARPRGSEWKGRGRR RRRADPEARTPPRRPRHAAGEVRRPPPASPPWR CCGAPRSSPPRSWTPLTARPQTRSWWPRPRRWAG STCTRGCCAPASPGARPSVPRRSRDAWLRCARC CCAWAMSWR*SGPASTATWRVSCTSPCSLSLW* PMRSWPWLATSSLQASRGARWCPCMRWPRGWPWT V*GRPSLPWSTPSWTAWGSSCARPWQPGCGDAA DGLMSSSVWSAQTLASAPTGWWLHSAASAAS*R LPSSCCCQRDELPTWQWPQPGPLGPTQEALSTRT HLPPQTQALRKG*VGRGFPEPGAGLWGSLRPSP LVSLLL*SLCFPFSFWGQEVRVNSQASDAGAQN TCSHLSPR*RGPGTPALT*APGEGAREHLLSPEP QVKGPGTPALT*APGEGAREHLLSPEPQVKGPR TPALT*APGEGARNTCSHLSPR*RGPGTPALT* APGEGARNTCSHLSPR*RGPEHLLSPEPPVKGPG TLALT*APGEGARNTSSHLSPR*RGPEHLLSPE PQVKGPGNTSHLNPGVPSQEEGPSQDMSPQGPC TFNLKVTLAWLKLEELWGLSL*TERKVHMLVA*S VSGGRV*HPLVMGSLCGGDRGRRAPGQHT*PMD VEPTAEKECCMSRIPVHCQWRVRVTPSAIFVLI LIQPFGDVPIRAP*ELRCLGSPAPQVPPHTAFP* RLHF*AHVRHLSWSPEPALSLPSAAWHSGDTGW TWGSRSGEAWPSSHPPEPSRPGFVLGWAMCQDR RVPAESQPRTHRDIEIPLEGRGGNKLLQTIVVIL DR

Sequence BOK.aAug05

GCCCCAAGCCCAGCCTCTCGCCAGCTGGGAGTCGCGCGCTGCCCACCTCGCTGCCCAGGCCCCCGACGCCGCGGCAGGAGCCCCCCAAGAGCGCGGGAAG CCCCGTGGACCTGGCGCTCCCGGCTCGGGCGTGGACGGGGCGGGCGCCGGGGCGGGGCGCGCGTCCTCGCGGGTCTGAATGGAAGGGTCGAGGTCGTCGT CGGCGGCGAGCAGATCCTGAAGCCAGAACTCCACCCCGGCGCCCGCGCCATGCGGCGGGAGAGGTGCGGCGCCCCCCACCCGCGTCGCCGCCATGGAGGT GCTGCGGCGCTCCTCGGTCTTCGCCGCCGAGATCATGGACGCCTTTGACCGCTCGCCCACAGACAAGGAGCTGGTGGCCCAGGCCAAGGCGCTGGGCCGG GAGTACGTGCACGCGCGGCTGCTGCGCGCCGGCCTCTCCTGGAGCGCGCCCGAGCGTGCCGCGCCGGTCCCGGGACGCCTGGCTGAGGTGTGCGCGGTGC TGCTGCGCCTGGGCGATGAGCTGGAGATGATCCGGCCCAGCGTCTACCGCAACGTGGCGCGTCAGCTGCACATCTCCCTGCAGTCTGAGCCTGTGGTGAC CGATGCGTTCCTGGCCGTGGCTGGCCACATCTTCTCTGCAGGCATCACGTGGGGCAAGGTGGTGTCCCTGTATGCGGTGGCCGCGGGGCTGGCCGTGGAC TGTGTGAGGCAGGCCCAGCCTGCCATGGTCCACGCCCTCGTGGACTGCCTGGGGGAGTTCGTGCGCAAGACCCTGGCAACCTGGCTGCGGAGACGCGGCG GATGGACTGATGTCCTCAAGTGTGTGGTCAGCACAGACCCTGGCCTCCGCTCCCACTGGCTGGTGGCTGCACTCTGCAGCTTCGGCCGCTTCCTGAAGGC TGCCTTCTTCGTGCTGCTGCCAGAGAGATGAGCTGCCCACCTGGCAGTGGCCGCAGCCTGGCCCTCTGGGCCCAACGCAGGAGGCCCTCAGCACCCGAAC ACATCTTCCTCCTCCCCACCCGAGCCTGGAGCACTCTAACCCTCGGAGACCCCCTAAGCCCCGTTCCTCCGCAGACCCAGGCCCTCCGGAAGGGGTGAGT GGGGAGGGGCTTTCCTGAGCCTGGAGCTGGGCTTTGGGGCAGCCTGCGACCCTCCCCGCTTGTGTCCCTTCTCCTGTGATCTCTGTGTTTTCCCTTTTCT TTCTGGGGCCAGGAAGTCAGGGTCAACTCCCAGGCCTCAGATGCAGGGGCCCAGAACACCTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGGAACAC CTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGGAACACCTGCTCTCACCTGAACCCCAGGTGAAGGGGCCCGGAACACCTGCTCTCACCTGAGCCCC AGGTGAAGGGGCCCGGAACACCTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGGAACACCTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGGAA CACCTGCTCTCACCTGAACCCCAGGTGAAGGGGCCCAGAACACCTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGAACACCTGCTCTCACCTGAGCC CCAGGTGAAGGGGCCCGGGAACACCTGCTCTCACCTGAGCCCCTGGTGAAGGGGCCCGGAACACTTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGA ACACCTGCTCTCACCTGAGCCCCCGGTGAAGGGGCCCGGAACACTTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGAACACCTCCTCTCACCTGAGC CCCAGGTGAAGGGGCCCGGAACACCTCCTGTCACCTGAGCCCCAGGTGAAGGGGCCCGGGAACACCTCTCACCTGAACCCGGGGGTCCCATCCCAGGAAG AAGGGCCATCTCAGGACATGAGTCCTCAGGGGCCCTGCACATTCAATCTGAAGGTGACCCTGGCCTGGCTGAAGCTGGAAGAGCTGTGGGGACTCAGCCT GTAAACAGAGCGTAAGGTTCACATGCTGGTTGCTTAATCCGTTTCTGGAGGAAGAGTATGACACCCACTTGTGATGGGGTCCTTGTGCGGTGGGGACCGG GGCCGGCGGGCTCCAGGCCAGCACACCTAACCCATGGATGTGGAACCTACGGCCGAGAAGGAATGTTGCATGAGTCGGATCCCAGTCCATTGTCAGTGGA GGGTGAGGGTGACCCCATCTGCTATTTTTGTGCTCATCCTCATACAACCATTTGGGGATGTGCCTATTAGGGCTCCGTAAGAACTCAGATGCCTGGGAAG CCCAGCCCCTCAGGTGCCCCCACACACAGCCTTCCCTTGACGCCTACATTTCTAGGCACATGTGAGGCATCTTTCCTGGAGCCCCGAGCCAGCCCTGTCC CTCCCCAGTGCAGCATGGCACTCAGGAGATACAGGCTGGACATGGGGCAGTCGTTCTGGGGAGGCCTGGCCTAGCAGCCACCCACCTGAGCCCTCCCGGC CAGGCTTCGTGCTGGGGTGGGCCATGTGCCAGGACAGGAGGGTCCCGGCGGAAAGCCAGCCCCGGACTCATCGTGACATTGAGATCCCACTGGAGGGTAG GGGTGGTAATAAACTTCTCCAAACGATCGTTGTCATTTTAGACAG

BOK.F4 (forward primer)
BOK.R6 (reverse primer)
Single exon skipping

Frame 0

APSPASRQLGVARCPPRCPGPRRRGRSPPRARE APWTWRSRLGRGRGGRRGGARVLAGLNGRVEVVV GGEQILKPELHPGARAMRRERCGAPHPRRRHGG AAALLGLRRRDHGRL*PLAHRQGAGGPGQGAGP GVRARAAAARRPLLERARACRAGPGTPG*GVRGA AAPGR*AGDDPAQRLPQRGASAAHLPAV*ACGD RCVPGRGWPHLLCRHHVGQGGVPVCGGRGAGRG LCEAGPACHGPRPRGLPGGVRAQDPGNLAAETRR MD*CPQVCGQHRPWPPLPLAGGCTLQLRPLPEG CLLRAAAREMSCPPGSGRSLALWAQRRRPSAPE HIFLLPTRAWSTLTLGDPLSPVPPQTQALRKG*V GRGFPEPGAGLWGSLRPSPLVSLLL*SLCFPFS FWGQEVRVNSQASDAGAQNTCSHLSPR*RGPGT PALT*APGEGAREHLLSPEPQVKGPGTPALT*AP GEGARNTCSHLSPR*RGPGTPALT*APGEGARE HLLSPEPQVKGPRTPALT*APGEGARNTCSHLS PR*RGPGTPALT*APGEGARNTCSHLSPR*RGPE HLLSPEPPVKGPGTLALT*APGEGARNTSSHLS PR*RGPEHLLSPEPQVKGPGNTSHLNPGVPSQE EGPSQDMSPQGPCTFNLKVTLAWLKLEELWGLSL *TERKVHMLVA*SVSGGRV*HPLVMGSLCGGDR GRRAPGQHT*PMDVEPTAEKECCMSRIPVHCQW RVRVTPSAIFVLILIQPFGDVPIRAP*ELRCLGS PAPQVPPHTAFP*RLHF*AHVRHLSWSPEPALS LPSAAWHSGDTGWTWGSRSGEAWPSSHPPEPSR PGFVLGWAMCQDRRVPAESQPRTHRDIEIPLEGR GGNKLLQTIVVILD

Frame 1

PQAQPLASWESRAAHLAAQAPDAAAGAPQERGK PRGPGAPGSGVDGAGAGAGRASSRV*MEGSRSS SAASRS*SQNSTPAPAPCGGRGAAPPTRVAAMEV LRRSSVFAAEIMDAFDRSPTDKELVAQAKALGR EYVHARLLRAGLSWSAPERAAPVPGRLAEVCAV LLRLGDELEMIRPSVYRNVARQLHISLQSEPVVT DAFLAVAGHIFSAGITWGKVVSLYAVAAGLAVD CVRQAQPAMVHALVDCLGEFVRKTLATWLRRRG GWTDVLKCVVSTDPGLRSHWLVAALCSFGRFLKA AFFVLLPER*AAHLAVAAAWPSGPNAGGPQHPN TSSSSPPEPGAL*PSETP*APFLRRPRPSGRGE WGGAFLSLELGFGAACDPPRLCPFSCDLCVFPFL SGARKSGSTPRPQMQGPRTPALT*APGEGAREH LLSPEPQVKGPGNTCSHLNPR*RGPEHLLSPEP QVKGPGTPALT*APGEGAREHLLSPEPQVKGPGN TCSHLNPR*RGPEHLLSPEPQVKGPGTPALT*A PGEGAREHLLSPEPLVKGPGTLALT*APGEGAR NTCSHLSPR*RGPEHLLSPEPQVKGPGTPPLT*A PGEGARNTSCHLSPR*RGPGTPLT*TRGSHPRK KGHLRT*VLRGPAHSI*R*PWPG*SWKSCGDSA CKQSVRFTCWLLNPFLEEEYDTHL*WGPCAVGTG AGGLQASTPNPWMWNLRPRRNVA*VGSQSIVSG G*G*PHLLFLCSSSYNHLGMCLLGLRKNSDAWE AQPLRCPHTQPSLDAYISRHM*GIFPGAPSQPCP SPVQHGTQEIQAGHGAVVLGRPGLAATHLSPPG QASCWGGPCARTGGSRRKASPGLIVTLRSHWRV GVVINFSKRSLSF*T

Frame 2

PKPSLSPAGSRALPTSLPRPPTPRQEPPKSAGS PVDLALPARAWTGRAPGRGARPRGSEWKGRGRR RRRADPEARTPPRRPRHAAGEVRRPPPASPPWR CCGAPRSSPPRSWTPLTARPQTRSWWPRPRRWAG STCTRGCCAPASPGARPSVPRRSRDAWLRCARC CCAWAMSWR*SGPASTATWRVSCTSPCSLSLW* PMRSWPWLATSSLQASRGARWCPCMRWPRGWPWT V*GRPSLPWSTPSWTAWGSSCARPWQPGCGDAA DGLMSSSVWSAQTLASAPTGWWLHSAASAAS*R LPSSCCCQRDELPTWQWPQPGPLGPTQEALSTRT HLPPPHPSLEHSNPRRPPKPRSSADPGPPEGVS GEGLS*AWSWALGQPATLPACVPSPVISVFSLF FLGPGSQGQLPGLRCRGPEHLLSPEPQVKGPGNT CSHLSPR*RGPGTPALT*TPGEGARNTCSHLSP R*RGPEHLLSPEPQVKGPGNTCSHLSPR*RGPG TPALT*TPGEGAQNTCSHLSPR*RGPEHLLSPEP QVKGPGNTCSHLSPW*RGPEHLLSPEPQVKGPG TPALT*APGEGARNTCSHLSPR*RGPEHLLSPE PQVKGPGTPPVT*APGEGAREHLSPEPGGPIPGR RAISGHESSGALHIQSEGDPGLAEAGRAVGTQP VNRA*GSHAGCLIRFWRKSMTPTCDGVLVRWGP GPAGSRPAHLTHGCGTYGREGMLHESDPSPLSVE GEGDPICYFCAHPHTTIWGCAY*GSVRTQMPGK PSPSGAPTHSLPLTPTFLGTCEASFLEPRASPV PPQCSMALRRYRLDMGQSFWGGLA*QPPT*ALPA RLRAGVGHVPGQEGPGGKPAPDSS*H*DPTGG* GW**TSPNDRCHFRQ

Sequence BOK.cAug05

GCCCCAAGCCCAGCCTCTCGCCAGCTGGGAGTCGCGCGCTGCCCACCTCGCTGCCCAGGCCCCCGACGCCGCGGCAGGAGCCCCCCAAGAGCGCGGGAAG CCCCGTGGACCTGGCGCTCCCGGCTCGGGCGTGGACGGGGCGGGCGCCGGGGCGGGGCGCGCGTCCTCGCGGGTCTGAATGGAAGGGTCGAGGTCGTCGT CGGCGGCGAGCAGATCCTGAAGCCAGAACTCCACCCCGGCGCCCGCGCCATGCGGCGGGAGAGGTGCGGCGCCCCCCACCCGCGTCGCCGCCATGGAGGT GCTGCGGCGCTCCTCGGTCTTCGCCGCCGAGATCATGGACGCCTTTGACCGCTCGCCCACAGACAAGGAGCTGGTGGCCCAGGCCAAGGCGCTGGGCCGG GAGTACGTGCACGCGCGGCTGCTGCGCGCCGGCCTCTCCTGGAGCGCGCCCGAGCGTGCCGCGCCGGTCCCGGGACGCCTGGCTGAGGTGTGCGCGGTGC TGCTGCGCCTGGATTCTTGCTGTTACTGGCCGAGATCCCGCCCGCTGCCCTCTGGATTCCGAGGCCTCCGAGGCCCCCCCATTGGAAACTGCGCCCACCG GGCTGGGTAGCCAAAACCTCAGTATTCGTTGGTGCTGTGAGGACATCTGGGGGTGCTCGGTTCTGGGGTCACACACGGTCCACTGGCTGCCCCCACCGTC TCCAAGGCGATGAGCTGGAGATGATCCGGCCCAGCGTCTACCGCAACGTGGCGCGTCAGCTGCACATCTCCCTGCAGTCTGAGCCTGTGGTGACCGATGC GTTCCTGGCCGTGGCTGGCCACATCTTCTCTGCAGGCATCACGTGGGGCAAGGTGGTGTCCCTGTATGCGGTGGCCGCGGGGCTGGCCGTGGACTGTGTG AGGCAGGCCCAGCCTGCCATGGTCCACGCCCTCGTGGACTGCCTGGGGGAGTTCGTGCGCAAGACCCTGGCAACCTGGCTGCGGAGACGCGGCGGATGGA CTGATGTCCTCAAGTGTGTGGTCAGCACAGACCCTGGCCTCCGCTCCCACTGGCTGGTGGCTGCACTCTGCAGCTTCGGCCGCTTCCTGAAGGCTGCCTT CTTCGTGCTGCTGCCAGAGAGATGAGCTGCCCACCTGGCAGTGGCCGCAGCCTGGCCCTCTGGGCCCAACGCAGGAGGCCCTCAGCACCCGAACACATCT TCCTCCTCCCCACCCGAGCCTGGAGCACTCTAACCCTCGGAGACCCCCTAAGCCCCGTTCCTCCGCAGACCCAGGCCCTCCGGAAGGGGTGAGTGGGGAG GGGCTTTCCTGAGCCTGGAGCTGGGCTTTGGGGCAGCCTGCGACCCTCCCCGCTTGTGTCCCTTCTCCTGTGATCTCTGTGTTTTCCCTTTTCTTTCTGG GGCCAGGAAGTCAGGGTCAACTCCCAGGCCTCAGATGCAGGGGCCCAGAACACCTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGGAACACCTGCTC TCACCTGAGCCCCAGGTGAAGGGGCCCGGGAACACCTGCTCTCACCTGAACCCCAGGTGAAGGGGCCCGGAACACCTGCTCTCACCTGAGCCCCAGGTGA AGGGGCCCGGAACACCTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGGAACACCTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGGAACACCTG CTCTCACCTGAACCCCAGGTGAAGGGGCCCAGAACACCTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGAACACCTGCTCTCACCTGAGCCCCAGGT GAAGGGGCCCGGGAACACCTGCTCTCACCTGAGCCCCTGGTGAAGGGGCCCGGAACACTTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGAACACCT GCTCTCACCTGAGCCCCCGGTGAAGGGGCCCGGAACACTTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGAACACCTCCTCTCACCTGAGCCCCAGG TGAAGGGGCCCGGAACACCTCCTGTCACCTGAGCCCCAGGTGAAGGGGCCCGGGAACACCTCTCACCTGAACCCGGGGGTCCCATCCCAGGAAGAAGGGC CATCTCAGGACATGAGTCCTCAGGGGCCCTGCACATTCAATCTGAAGGTGACCCTGGCCTGGCTGAAGCTGGAAGAGCTGTGGGGACTCAGCCTGTAAAC AGAGCGTAAGGTTCACATGCTGGTTGCTTAATCCGTTTCTGGAGGAAGAGTATGACACCCACTTGTGATGGGGTCCTTGTGCGGTGGGGACCGGGGCCGG CGGGCTCCAGGCCAGCACACCTAACCCATGGATGTGGAACCTACGGCCGAGAAGGAATGTTGCATGAGTCGGATCCCAGTCCATTGTCAGTGGAGGGTGA GGGTGACCCCATCTGCTATTTTTGTGCTCATCCTCATACAACCATTTGGGGATGTGCCTATTAGGGCTCCGTAAGAACTCAGATGCCTGGGAAGCCCAGC CCCTCAGGTGCCCCCACACACAGCCTTCCCTTGACGCCTACATTTCTAGGCACATGTGAGGCATCTTTCCTGGAGCCCCGAGCCAGCCCTGTCCCTCCCC AGTGCAGCATGGCACTCAGGAGATACAGGCTGGACATGGGGCAGTCGTTCTGGGGAGGCCTGGCCTAGCAGCCACCCACCTGAGCCCTCCCGGCCAGGCT TCGTGCTGGGGTGGGCCATGTGCCAGGACAGGAGGGTCCCGGCGGAAAGCCAGCCCCGGACTCATCGTGACATTGAGATCCCACTGGAGGGTAGGGGTGG TAATAAACTTCTCCAAACGATCGTTGTCATTTTAGACAG

BOK.F4 (forward primer)
BOK.R6 (reverse primer)
Single exon skipping

Frame 0

APSPASRQLGVARCPPRCPGPRRRGRSPPRARE APWTWRSRLGRGRGGRRGGARVLAGLNGRVEVVV GGEQILKPELHPGARAMRRERCGAPHPRRRHGG AAALLGLRRRDHGRL*PLAHRQGAGGPGQGAGP GVRARAAAARRPLLERARACRAGPGTPG*GVRGA AAPGFLLLLAEIPPAALWIPRPPRPPHWKLRPP GWVAKTSVFVGAVRTSGGARFWGHTRSTGCPHR LQGDELEMIRPSVYRNVARQLHISLQSEPVVTDA FLAVAGHIFSAGITWGKVVSLYAVAAGLAVDCV RQAQPAMVHALVDCLGEFVRKTLATWLRRRGGW TDVLKCVVSTDPGLRSHWLVAALCSFGRFLKAAF FVLLPER*AAHLAVAAAWPSGPNAGGPQHPNTS SSSPPEPGAL*PSETP*APFLRRPRPSGRGEWG GAFLSLELGFGAACDPPRLCPFSCDLCVFPFLSG ARKSGSTPRPQMQGPRTPALT*APGEGAREHLL SPEPQVKGPGNTCSHLNPR*RGPEHLLSPEPQV KGPGTPALT*APGEGAREHLLSPEPQVKGPGNTC SHLNPR*RGPEHLLSPEPQVKGPGTPALT*APG EGAREHLLSPEPLVKGPGTLALT*APGEGARNT CSHLSPR*RGPEHLLSPEPQVKGPGTPPLT*APG EGARNTSCHLSPR*RGPGTPLT*TRGSHPRKKG HLRT*VLRGPAHSI*R*PWPG*SWKSCGDSACK QSVRFTCWLLNPFLEEEYDTHL*WGPCAVGTGAG GLQASTPNPWMWNLRPRRNVA*VGSQSIVSGG* G*PHLLFLCSSSYNHLGMCLLGLRKNSDAWEAQ PLRCPHTQPSLDAYISRHM*GIFPGAPSQPCPSP VQHGTQEIQAGHGAVVLGRPGLAATHLSPPGQA SCWGGPCARTGGSRRKASPGLIVTLRSHWRVGV VINFSKRSLSF*T

Frame 1

PQAQPLASWESRAAHLAAQAPDAAAGAPQERGK PRGPGAPGSGVDGAGAGAGRASSRV*MEGSRSS SAASRS*SQNSTPAPAPCGGRGAAPPTRVAAMEV LRRSSVFAAEIMDAFDRSPTDKELVAQAKALGR EYVHARLLRAGLSWSAPERAAPVPGRLAEVCAV LLRLDSCCYWPRSRPLPSGFRGLRGPPIGNCAHR AG*PKPQYSLVL*GHLGVLGSGVTHGPLAAPTV SKAMSWR*SGPASTATWRVSCTSPCSLSLW*PM RSWPWLATSSLQASRGARWCPCMRWPRGWPWTV* GRPSLPWSTPSWTAWGSSCARPWQPGCGDAADG LMSSSVWSAQTLASAPTGWWLHSAASAAS*RLP SSCCCQRDELPTWQWPQPGPLGPTQEALSTRTHL PPPHPSLEHSNPRRPPKPRSSADPGPPEGVSGE GLS*AWSWALGQPATLPACVPSPVISVFSLFFL GPGSQGQLPGLRCRGPEHLLSPEPQVKGPGNTCS HLSPR*RGPGTPALT*TPGEGARNTCSHLSPR* RGPEHLLSPEPQVKGPGNTCSHLSPR*RGPGTP ALT*TPGEGAQNTCSHLSPR*RGPEHLLSPEPQV KGPGNTCSHLSPW*RGPEHLLSPEPQVKGPGTP ALT*APGEGARNTCSHLSPR*RGPEHLLSPEPQ VKGPGTPPVT*APGEGAREHLSPEPGGPIPGRRA ISGHESSGALHIQSEGDPGLAEAGRAVGTQPVN RA*GSHAGCLIRFWRKSMTPTCDGVLVRWGPGP AGSRPAHLTHGCGTYGREGMLHESDPSPLSVEGE GDPICYFCAHPHTTIWGCAY*GSVRTQMPGKPS PSGAPTHSLPLTPTFLGTCEASFLEPRASPVPP QCSMALRRYRLDMGQSFWGGLA*QPPT*ALPARL RAGVGHVPGQEGPGGKPAPDSS*H*DPTGG*GW **TSPNDRCHFRQ

Frame 2

PKPSLSPAGSRALPTSLPRPPTPRQEPPKSAGS PVDLALPARAWTGRAPGRGARPRGSEWKGRGRR RRRADPEARTPPRRPRHAAGEVRRPPPASPPWR CCGAPRSSPPRSWTPLTARPQTRSWWPRPRRWAG STCTRGCCAPASPGARPSVPRRSRDAWLRCARC CCAWILAVTGRDPARCPLDSEASEAPPLETAPT GLGSQNLSIRWCCEDIWGCSVLGSHTVHWLPPPS PRR*AGDDPAQRLPQRGASAAHLPAV*ACGDRC VPGRGWPHLLCRHHVGQGGVPVCGGRGAGRGLC EAGPACHGPRPRGLPGGVRAQDPGNLAAETRRMD *CPQVCGQHRPWPPLPLAGGCTLQLRPLPEGCL LRAAAREMSCPPGSGRSLALWAQRRRPSAPEHI FLLPTRAWSTLTLGDPLSPVPPQTQALRKG*VGR GFPEPGAGLWGSLRPSPLVSLLL*SLCFPFSFW GQEVRVNSQASDAGAQNTCSHLSPR*RGPGTPA LT*APGEGAREHLLSPEPQVKGPGTPALT*APGE GARNTCSHLSPR*RGPGTPALT*APGEGAREHL LSPEPQVKGPRTPALT*APGEGARNTCSHLSPR *RGPGTPALT*APGEGARNTCSHLSPR*RGPEHL LSPEPPVKGPGTLALT*APGEGARNTSSHLSPR *RGPEHLLSPEPQVKGPGNTSHLNPGVPSQEEG PSQDMSPQGPCTFNLKVTLAWLKLEELWGLSL*T ERKVHMLVA*SVSGGRV*HPLVMGSLCGGDRGR RAPGQHT*PMDVEPTAEKECCMSRIPVHCQWRV RVTPSAIFVLILIQPFGDVPIRAP*ELRCLGSPA PQVPPHTAFP*RLHF*AHVRHLSWSPEPALSLP SAAWHSGDTGWTWGSRSGEAWPSSHPPEPSRPG FVLGWAMCQDRRVPAESQPRTHRDIEIPLEGRGG NKLLQTIVVILD