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Sequences

Sequence BOK.dAug05

GCCCCAAGCCCAGCCTCTCGCCAGCTGGGAGTCGCGCGCTGCCCACCTCGCTGCCCAGGCCCCCGACGCCGCGGCAGGAGCCCCCCAAGAGCGCGGGAAG CCCCGTGGACCTGGCGCTCCCGGCTCGGGCGTGGACGGGGCGGGCGCCGGGGCGGGGCGCGCGTCCTCGCGGGTCTGAATGGAAGGGTCGAGGTCGTCGT CGGCGGCGAGCAGATCCTGAAGCCAGAACTCCACCCCGGCGCCCGCGCCATGCGGCGGGAGAGGTGCGGCGCCCCCCACCCGCGTCGCCGCCATGGAGGT GCTGCGGCGCTCCTCGGTCTTCGCCGCCGAGATCATGGACGCCTTTGACCGCTCGCCCACAGACAAGGAGCTGGTGGCCCAGGCCAAGGCGCTGGGCCGG GAGTACGTGCACGCGCGGCTGCTGCGCGCCGGCCTCTCCTGGAGCGCGCCCGAGCGTGCCGCGCCGGTCCCGGGACGCCTGGCTGAGGTGTGCGCGGTGC TGCTGCGCCTGGGCATCACGTGGGGCAAGGTGGTGTCCCTGTATGCGGTGGCCGCGGGGCTGGCCGTGGACTGTGTGAGGCAGGCCCAGCCTGCCATGGT CCACGCCCTCGTGGACTGCCTGGGGGAGTTCGTGCGCAAGACCCTGGCAACCTGGCTGCGGAGACGCGGCGGATGGACTGATGTCCTCAAGTGTGTGGTC AGCACAGACCCTGGCCTCCGCTCCCACTGGCTGGTGGCTGCACTCTGCAGCTTCGGCCGCTTCCTGAAGGCTGCCTTCTTCGTGCTGCTGCCAGAGAGAT GAGCTGCCCACCTGGCAGTGGCCGCAGCCTGGCCCTCTGGGCCCAACGCAGGAGGCCCTCAGCACCCGAACACATCTTCCTCCTCCCCACCCGAGCCTGG AGCACTCTAACCCTCGGAGACCCCCTAAGCCCCGTTCCTCCGCAGACCCAGGCCCTCCGGAAGGGGTGAGTGGGGAGGGGCTTTCCTGAGCCTGGAGCTG GGCTTTGGGGCAGCCTGCGACCCTCCCCGCTTGTGTCCCTTCTCCTGTGATCTCTGTGTTTTCCCTTTTCTTTCTGGGGCCAGGAAGTCAGGGTCAACTC CCAGGCCTCAGATGCAGGGGCCCAGAACACCTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGGAACACCTGCTCTCACCTGAGCCCCAGGTGAAGGG GCCCGGGAACACCTGCTCTCACCTGAACCCCAGGTGAAGGGGCCCGGAACACCTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGAACACCTGCTCTC ACCTGAGCCCCAGGTGAAGGGGCCCGGGAACACCTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGGAACACCTGCTCTCACCTGAACCCCAGGTGAA GGGGCCCAGAACACCTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGAACACCTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGGAACACCTGCT CTCACCTGAGCCCCTGGTGAAGGGGCCCGGAACACTTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGAACACCTGCTCTCACCTGAGCCCCCGGTGA AGGGGCCCGGAACACTTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGAACACCTCCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGAACACCTCCT GTCACCTGAGCCCCAGGTGAAGGGGCCCGGGAACACCTCTCACCTGAACCCGGGGGTCCCATCCCAGGAAGAAGGGCCATCTCAGGACATGAGTCCTCAG GGGCCCTGCACATTCAATCTGAAGGTGACCCTGGCCTGGCTGAAGCTGGAAGAGCTGTGGGGACTCAGCCTGTAAACAGAGCGTAAGGTTCACATGCTGG TTGCTTAATCCGTTTCTGGAGGAAGAGTATGACACCCACTTGTGATGGGGTCCTTGTGCGGTGGGGACCGGGGCCGGCGGGCTCCAGGCCAGCACACCTA ACCCATGGATGTGGAACCTACGGCCGAGAAGGAATGTTGCATGAGTCGGATCCCAGTCCATTGTCAGTGGAGGGTGAGGGTGACCCCATCTGCTATTTTT GTGCTCATCCTCATACAACCATTTGGGGATGTGCCTATTAGGGCTCCGTAAGAACTCAGATGCCTGGGAAGCCCAGCCCCTCAGGTGCCCCCACACACAG CCTTCCCTTGACGCCTACATTTCTAGGCACATGTGAGGCATCTTTCCTGGAGCCCCGAGCCAGCCCTGTCCCTCCCCAGTGCAGCATGGCACTCAGGAGA TACAGGCTGGACATGGGGCAGTCGTTCTGGGGAGGCCTGGCCTAGCAGCCACCCACCTGAGCCCTCCCGGCCAGGCTTCGTGCTGGGGTGGGCCATGTGC CAGGACAGGAGGGTCCCGGCGGAAAGCCAGCCCCGGACTCATCGTGACATTGAGATCCCACTGGAGGGTAGGGGTGGTAATAAACTTCTCCAAACGATCG TTGTCATTTTAGACAG

BOK.F4 (forward primer)
BOK.R6 (reverse primer)
Single exon skipping

Frame 0

APSPASRQLGVARCPPRCPGPRRRGRSPPRARE APWTWRSRLGRGRGGRRGGARVLAGLNGRVEVVV GGEQILKPELHPGARAMRRERCGAPHPRRRHGG AAALLGLRRRDHGRL*PLAHRQGAGGPGQGAGP GVRARAAAARRPLLERARACRAGPGTPG*GVRGA AAPGHHVGQGGVPVCGGRGAGRGLCEAGPACHG PRPRGLPGGVRAQDPGNLAAETRRMD*CPQVCG QHRPWPPLPLAGGCTLQLRPLPEGCLLRAAAREM SCPPGSGRSLALWAQRRRPSAPEHIFLLPTRAW STLTLGDPLSPVPPQTQALRKG*VGRGFPEPGA GLWGSLRPSPLVSLLL*SLCFPFSFWGQEVRVNS QASDAGAQNTCSHLSPR*RGPGTPALT*APGEG AREHLLSPEPQVKGPGTPALT*APGEGARNTCS HLSPR*RGPGTPALT*APGEGAREHLLSPEPQVK GPRTPALT*APGEGARNTCSHLSPR*RGPGTPA LT*APGEGARNTCSHLSPR*RGPEHLLSPEPPV KGPGTLALT*APGEGARNTSSHLSPR*RGPEHLL SPEPQVKGPGNTSHLNPGVPSQEEGPSQDMSPQ GPCTFNLKVTLAWLKLEELWGLSL*TERKVHML VA*SVSGGRV*HPLVMGSLCGGDRGRRAPGQHT* PMDVEPTAEKECCMSRIPVHCQWRVRVTPSAIF VLILIQPFGDVPIRAP*ELRCLGSPAPQVPPHT AFP*RLHF*AHVRHLSWSPEPALSLPSAAWHSGD TGWTWGSRSGEAWPSSHPPEPSRPGFVLGWAMC QDRRVPAESQPRTHRDIEIPLEGRGGNKLLQTI VVILD

Frame 1

PQAQPLASWESRAAHLAAQAPDAAAGAPQERGK PRGPGAPGSGVDGAGAGAGRASSRV*MEGSRSS SAASRS*SQNSTPAPAPCGGRGAAPPTRVAAMEV LRRSSVFAAEIMDAFDRSPTDKELVAQAKALGR EYVHARLLRAGLSWSAPERAAPVPGRLAEVCAV LLRLGITWGKVVSLYAVAAGLAVDCVRQAQPAMV HALVDCLGEFVRKTLATWLRRRGGWTDVLKCVV STDPGLRSHWLVAALCSFGRFLKAAFFVLLPER *AAHLAVAAAWPSGPNAGGPQHPNTSSSSPPEPG AL*PSETP*APFLRRPRPSGRGEWGGAFLSLEL GFGAACDPPRLCPFSCDLCVFPFLSGARKSGST PRPQMQGPRTPALT*APGEGAREHLLSPEPQVKG PGNTCSHLNPR*RGPEHLLSPEPQVKGPGTPAL T*APGEGAREHLLSPEPQVKGPGNTCSHLNPR* RGPEHLLSPEPQVKGPGTPALT*APGEGAREHLL SPEPLVKGPGTLALT*APGEGARNTCSHLSPR* RGPEHLLSPEPQVKGPGTPPLT*APGEGARNTS CHLSPR*RGPGTPLT*TRGSHPRKKGHLRT*VLR GPAHSI*R*PWPG*SWKSCGDSACKQSVRFTCW LLNPFLEEEYDTHL*WGPCAVGTGAGGLQASTP NPWMWNLRPRRNVA*VGSQSIVSGG*G*PHLLFL CSSSYNHLGMCLLGLRKNSDAWEAQPLRCPHTQ PSLDAYISRHM*GIFPGAPSQPCPSPVQHGTQE IQAGHGAVVLGRPGLAATHLSPPGQASCWGGPCA RTGGSRRKASPGLIVTLRSHWRVGVVINFSKRS LSF*T

Frame 2

PKPSLSPAGSRALPTSLPRPPTPRQEPPKSAGS PVDLALPARAWTGRAPGRGARPRGSEWKGRGRR RRRADPEARTPPRRPRHAAGEVRRPPPASPPWR CCGAPRSSPPRSWTPLTARPQTRSWWPRPRRWAG STCTRGCCAPASPGARPSVPRRSRDAWLRCARC CCAWASRGARWCPCMRWPRGWPWTV*GRPSLPW STPSWTAWGSSCARPWQPGCGDAADGLMSSSVWS AQTLASAPTGWWLHSAASAAS*RLPSSCCCQRD ELPTWQWPQPGPLGPTQEALSTRTHLPPPHPSL EHSNPRRPPKPRSSADPGPPEGVSGEGLS*AWSW ALGQPATLPACVPSPVISVFSLFFLGPGSQGQL PGLRCRGPEHLLSPEPQVKGPGNTCSHLSPR*R GPGTPALT*TPGEGARNTCSHLSPR*RGPEHLLS PEPQVKGPGNTCSHLSPR*RGPGTPALT*TPGE GAQNTCSHLSPR*RGPEHLLSPEPQVKGPGNTC SHLSPW*RGPEHLLSPEPQVKGPGTPALT*APGE GARNTCSHLSPR*RGPEHLLSPEPQVKGPGTPP VT*APGEGAREHLSPEPGGPIPGRRAISGHESS GALHIQSEGDPGLAEAGRAVGTQPVNRA*GSHAG CLIRFWRKSMTPTCDGVLVRWGPGPAGSRPAHL THGCGTYGREGMLHESDPSPLSVEGEGDPICYF CAHPHTTIWGCAY*GSVRTQMPGKPSPSGAPTHS LPLTPTFLGTCEASFLEPRASPVPPQCSMALRR YRLDMGQSFWGGLA*QPPT*ALPARLRAGVGHV PGQEGPGGKPAPDSS*H*DPTGG*GW**TSPNDR CHFRQ

Sequence BOK.bAug05

GCCCCAAGCCCAGCCTCTCGCCAGCTGGGAGTCGCGCGCTGCCCACCTCGCTGCCCAGGCCCCCGACGCCGCGGCAGGAGCCCCCCAAGAGCGCGGGAAG CCCCGTGGACCTGGCGCTCCCGGCTCGGGCGTGGACGGGGCGGGCGCCGGGGCGGGGCGCGCGTCCTCGCGGGTCTGAATGGAAGGGTCGAGGTCGTCGT CGGCGGCGAGCAGATCCTGAAGCCAGAACTCCACCCCGGCGCCCGCGCCATGCGGCGGGAGAGGTGCGGCGCCCCCCACCCGCGTCGCCGCCATGGAGGT GCTGCGGCGCTCCTCGGTCTTCGCCGCCGAGATCATGGACGCCTTTGACCGCTCGCCCACAGACAAGGAGCTGGTGGCCCAGGCCAAGGCGCTGGGCCGG GAGTACGTGCACGCGCGGCTGCTGCGCGCCGGCCTCTCCTGGAGCGCGCCCGAGCGTGCCGCGCCGGTCCCGGGACGCCTGGCTGAGGTGTGCGCGGTGC TGCTGCGCCTGGGCGATGAGCTGGAGATGATCCGGCCCAGCGTCTACCGCAACGTGGCGCGTCAGCTGCACATCTCCCTGCAGTCTGAGCCTGTGGTGAC CGATGCGTTCCTGGCCGTGGCTGGCCACATCTTCTCTGCAGGCATCACGTGGGGCAAGGTGGTGTCCCTGTATGCGGTGGCCGCGGGGCTGGCCGTGGAC TGTGTGAGGCAGGCCCAGCCTGCCATGGTCCACGCCCTCGTGGACTGCCTGGGGGAGTTCGTGCGCAAGACCCTGGCAACCTGGCTGCGGAGACGCGGCG GATGGACTGATGTCCTCAAGTGTGTGGTCAGCACAGACCCTGGCCTCCGCTCCCACTGGCTGGTGGCTGCACTCTGCAGCTTCGGCCGCTTCCTGAAGGC TGCCTTCTTCGTGCTGCTGCCAGAGAGATGAGCTGCCCACCTGGCAGTGGCCGCAGCCTGGCCCTCTGGGCCCAACGCAGGAGGCCCTCAGCACCCGAAC ACATCTTCCTCCGCAGACCCAGGCCCTCCGGAAGGGGTGAGTGGGGAGGGGCTTTCCTGAGCCTGGAGCTGGGCTTTGGGGCAGCCTGCGACCCTCCCCG CTTGTGTCCCTTCTCCTGTGATCTCTGTGTTTTCCCTTTTCTTTCTGGGGCCAGGAAGTCAGGGTCAACTCCCAGGCCTCAGATGCAGGGGCCCAGAACA CCTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGGAACACCTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGGAACACCTGCTCTCACCTGAACC CCAGGTGAAGGGGCCCGGAACACCTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGGAACACCTGCTCTCACCTGAACCCCAGGTGAAGGGGCCCAGA ACACCTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGAACACCTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGGAACACCTGCTCTCACCTGAG CCCCTGGTGAAGGGGCCCGGAACACTTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGAACACCTGCTCTCACCTGAGCCCCCGGTGAAGGGGCCCGG AACACTTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGAACACCTCCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGAACACCTCCTGTCACCTGAG CCCCAGGTGAAGGGGCCCGGGAACACCTCTCACCTGAACCCGGGGGTCCCATCCCAGGAAGAAGGGCCATCTCAGGACATGAGTCCTCAGGGGCCCTGCA CATTCAATCTGAAGGTGACCCTGGCCTGGCTGAAGCTGGAAGAGCTGTGGGGACTCAGCCTGTAAACAGAGCGTAAGGTTCACATGCTGGTTGCTTAATC CGTTTCTGGAGGAAGAGTATGACACCCACTTGTGATGGGGTCCTTGTGCGGTGGGGACCGGGGCCGGCGGGCTCCAGGCCAGCACACCTAACCCATGGAT GTGGAACCTACGGCCGAGAAGGAATGTTGCATGAGTCGGATCCCAGTCCATTGTCAGTGGAGGGTGAGGGTGACCCCATCTGCTATTTTTGTGCTCATCC TCATACAACCATTTGGGGATGTGCCTATTAGGGCTCCGTAAGAACTCAGATGCCTGGGAAGCCCAGCCCCTCAGGTGCCCCCACACACAGCCTTCCCTTG ACGCCTACATTTCTAGGCACATGTGAGGCATCTTTCCTGGAGCCCCGAGCCAGCCCTGTCCCTCCCCAGTGCAGCATGGCACTCAGGAGATACAGGCTGG ACATGGGGCAGTCGTTCTGGGGAGGCCTGGCCTAGCAGCCACCCACCTGAGCCCTCCCGGCCAGGCTTCGTGCTGGGGTGGGCCATGTGCCAGGACAGGA GGGTCCCGGCGGAAAGCCAGCCCCGGACTCATCGTGACATTGAGATCCCACTGGAGGGTAGGGGTGGTAATAAACTTCTCCAAACGATCGTTGTCATTTT AGACAGAA

BOK.F4 (forward primer)
BOK.R6 (reverse primer)
Single exon skipping

Frame 0

APSPASRQLGVARCPPRCPGPRRRGRSPPRARE APWTWRSRLGRGRGGRRGGARVLAGLNGRVEVVV GGEQILKPELHPGARAMRRERCGAPHPRRRHGG AAALLGLRRRDHGRL*PLAHRQGAGGPGQGAGP GVRARAAAARRPLLERARACRAGPGTPG*GVRGA AAPGR*AGDDPAQRLPQRGASAAHLPAV*ACGD RCVPGRGWPHLLCRHHVGQGGVPVCGGRGAGRG LCEAGPACHGPRPRGLPGGVRAQDPGNLAAETRR MD*CPQVCGQHRPWPPLPLAGGCTLQLRPLPEG CLLRAAAREMSCPPGSGRSLALWAQRRRPSAPE HIFLRRPRPSGRGEWGGAFLSLELGFGAACDPPR LCPFSCDLCVFPFLSGARKSGSTPRPQMQGPRT PALT*APGEGAREHLLSPEPQVKGPGNTCSHLN PR*RGPEHLLSPEPQVKGPGNTCSHLNPR*RGPE HLLSPEPQVKGPGTPALT*APGEGAREHLLSPE PLVKGPGTLALT*APGEGARNTCSHLSPR*RGP EHLLSPEPQVKGPGTPPLT*APGEGARNTSCHLS PR*RGPGTPLT*TRGSHPRKKGHLRT*VLRGPA HSI*R*PWPG*SWKSCGDSACKQSVRFTCWLLN PFLEEEYDTHL*WGPCAVGTGAGGLQASTPNPWM WNLRPRRNVA*VGSQSIVSGG*G*PHLLFLCSS SYNHLGMCLLGLRKNSDAWEAQPLRCPHTQPSL DAYISRHM*GIFPGAPSQPCPSPVQHGTQEIQAG HGAVVLGRPGLAATHLSPPGQASCWGGPCARTG GSRRKASPGLIVTLRSHWRVGVVINFSKRSLSF *TE

Frame 1

PQAQPLASWESRAAHLAAQAPDAAAGAPQERGK PRGPGAPGSGVDGAGAGAGRASSRV*MEGSRSS SAASRS*SQNSTPAPAPCGGRGAAPPTRVAAMEV LRRSSVFAAEIMDAFDRSPTDKELVAQAKALGR EYVHARLLRAGLSWSAPERAAPVPGRLAEVCAV LLRLGDELEMIRPSVYRNVARQLHISLQSEPVVT DAFLAVAGHIFSAGITWGKVVSLYAVAAGLAVD CVRQAQPAMVHALVDCLGEFVRKTLATWLRRRG GWTDVLKCVVSTDPGLRSHWLVAALCSFGRFLKA AFFVLLPER*AAHLAVAAAWPSGPNAGGPQHPN TSSSADPGPPEGVSGEGLS*AWSWALGQPATLP ACVPSPVISVFSLFFLGPGSQGQLPGLRCRGPEH LLSPEPQVKGPGNTCSHLSPR*RGPGTPALT*T PGEGARNTCSHLSPR*RGPGTPALT*TPGEGAQ NTCSHLSPR*RGPEHLLSPEPQVKGPGNTCSHLS PW*RGPEHLLSPEPQVKGPGTPALT*APGEGAR NTCSHLSPR*RGPEHLLSPEPQVKGPGTPPVT* APGEGAREHLSPEPGGPIPGRRAISGHESSGALH IQSEGDPGLAEAGRAVGTQPVNRA*GSHAGCLI RFWRKSMTPTCDGVLVRWGPGPAGSRPAHLTHG CGTYGREGMLHESDPSPLSVEGEGDPICYFCAHP HTTIWGCAY*GSVRTQMPGKPSPSGAPTHSLPL TPTFLGTCEASFLEPRASPVPPQCSMALRRYRL DMGQSFWGGLA*QPPT*ALPARLRAGVGHVPGQE GPGGKPAPDSS*H*DPTGG*GW**TSPNDRCHF RQ

Frame 2

PKPSLSPAGSRALPTSLPRPPTPRQEPPKSAGS PVDLALPARAWTGRAPGRGARPRGSEWKGRGRR RRRADPEARTPPRRPRHAAGEVRRPPPASPPWR CCGAPRSSPPRSWTPLTARPQTRSWWPRPRRWAG STCTRGCCAPASPGARPSVPRRSRDAWLRCARC CCAWAMSWR*SGPASTATWRVSCTSPCSLSLW* PMRSWPWLATSSLQASRGARWCPCMRWPRGWPWT V*GRPSLPWSTPSWTAWGSSCARPWQPGCGDAA DGLMSSSVWSAQTLASAPTGWWLHSAASAAS*R LPSSCCCQRDELPTWQWPQPGPLGPTQEALSTRT HLPPQTQALRKG*VGRGFPEPGAGLWGSLRPSP LVSLLL*SLCFPFSFWGQEVRVNSQASDAGAQN TCSHLSPR*RGPGTPALT*APGEGAREHLLSPEP QVKGPGTPALT*APGEGAREHLLSPEPQVKGPR TPALT*APGEGARNTCSHLSPR*RGPGTPALT* APGEGARNTCSHLSPR*RGPEHLLSPEPPVKGPG TLALT*APGEGARNTSSHLSPR*RGPEHLLSPE PQVKGPGNTSHLNPGVPSQEEGPSQDMSPQGPC TFNLKVTLAWLKLEELWGLSL*TERKVHMLVA*S VSGGRV*HPLVMGSLCGGDRGRRAPGQHT*PMD VEPTAEKECCMSRIPVHCQWRVRVTPSAIFVLI LIQPFGDVPIRAP*ELRCLGSPAPQVPPHTAFP* RLHF*AHVRHLSWSPEPALSLPSAAWHSGDTGW TWGSRSGEAWPSSHPPEPSRPGFVLGWAMCQDR RVPAESQPRTHRDIEIPLEGRGGNKLLQTIVVIL DR

Sequence BOK.aAug05

GCCCCAAGCCCAGCCTCTCGCCAGCTGGGAGTCGCGCGCTGCCCACCTCGCTGCCCAGGCCCCCGACGCCGCGGCAGGAGCCCCCCAAGAGCGCGGGAAG CCCCGTGGACCTGGCGCTCCCGGCTCGGGCGTGGACGGGGCGGGCGCCGGGGCGGGGCGCGCGTCCTCGCGGGTCTGAATGGAAGGGTCGAGGTCGTCGT CGGCGGCGAGCAGATCCTGAAGCCAGAACTCCACCCCGGCGCCCGCGCCATGCGGCGGGAGAGGTGCGGCGCCCCCCACCCGCGTCGCCGCCATGGAGGT GCTGCGGCGCTCCTCGGTCTTCGCCGCCGAGATCATGGACGCCTTTGACCGCTCGCCCACAGACAAGGAGCTGGTGGCCCAGGCCAAGGCGCTGGGCCGG GAGTACGTGCACGCGCGGCTGCTGCGCGCCGGCCTCTCCTGGAGCGCGCCCGAGCGTGCCGCGCCGGTCCCGGGACGCCTGGCTGAGGTGTGCGCGGTGC TGCTGCGCCTGGGCGATGAGCTGGAGATGATCCGGCCCAGCGTCTACCGCAACGTGGCGCGTCAGCTGCACATCTCCCTGCAGTCTGAGCCTGTGGTGAC CGATGCGTTCCTGGCCGTGGCTGGCCACATCTTCTCTGCAGGCATCACGTGGGGCAAGGTGGTGTCCCTGTATGCGGTGGCCGCGGGGCTGGCCGTGGAC TGTGTGAGGCAGGCCCAGCCTGCCATGGTCCACGCCCTCGTGGACTGCCTGGGGGAGTTCGTGCGCAAGACCCTGGCAACCTGGCTGCGGAGACGCGGCG GATGGACTGATGTCCTCAAGTGTGTGGTCAGCACAGACCCTGGCCTCCGCTCCCACTGGCTGGTGGCTGCACTCTGCAGCTTCGGCCGCTTCCTGAAGGC TGCCTTCTTCGTGCTGCTGCCAGAGAGATGAGCTGCCCACCTGGCAGTGGCCGCAGCCTGGCCCTCTGGGCCCAACGCAGGAGGCCCTCAGCACCCGAAC ACATCTTCCTCCTCCCCACCCGAGCCTGGAGCACTCTAACCCTCGGAGACCCCCTAAGCCCCGTTCCTCCGCAGACCCAGGCCCTCCGGAAGGGGTGAGT GGGGAGGGGCTTTCCTGAGCCTGGAGCTGGGCTTTGGGGCAGCCTGCGACCCTCCCCGCTTGTGTCCCTTCTCCTGTGATCTCTGTGTTTTCCCTTTTCT TTCTGGGGCCAGGAAGTCAGGGTCAACTCCCAGGCCTCAGATGCAGGGGCCCAGAACACCTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGGAACAC CTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGGAACACCTGCTCTCACCTGAACCCCAGGTGAAGGGGCCCGGAACACCTGCTCTCACCTGAGCCCC AGGTGAAGGGGCCCGGAACACCTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGGAACACCTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGGAA CACCTGCTCTCACCTGAACCCCAGGTGAAGGGGCCCAGAACACCTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGAACACCTGCTCTCACCTGAGCC CCAGGTGAAGGGGCCCGGGAACACCTGCTCTCACCTGAGCCCCTGGTGAAGGGGCCCGGAACACTTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGA ACACCTGCTCTCACCTGAGCCCCCGGTGAAGGGGCCCGGAACACTTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGAACACCTCCTCTCACCTGAGC CCCAGGTGAAGGGGCCCGGAACACCTCCTGTCACCTGAGCCCCAGGTGAAGGGGCCCGGGAACACCTCTCACCTGAACCCGGGGGTCCCATCCCAGGAAG AAGGGCCATCTCAGGACATGAGTCCTCAGGGGCCCTGCACATTCAATCTGAAGGTGACCCTGGCCTGGCTGAAGCTGGAAGAGCTGTGGGGACTCAGCCT GTAAACAGAGCGTAAGGTTCACATGCTGGTTGCTTAATCCGTTTCTGGAGGAAGAGTATGACACCCACTTGTGATGGGGTCCTTGTGCGGTGGGGACCGG GGCCGGCGGGCTCCAGGCCAGCACACCTAACCCATGGATGTGGAACCTACGGCCGAGAAGGAATGTTGCATGAGTCGGATCCCAGTCCATTGTCAGTGGA GGGTGAGGGTGACCCCATCTGCTATTTTTGTGCTCATCCTCATACAACCATTTGGGGATGTGCCTATTAGGGCTCCGTAAGAACTCAGATGCCTGGGAAG CCCAGCCCCTCAGGTGCCCCCACACACAGCCTTCCCTTGACGCCTACATTTCTAGGCACATGTGAGGCATCTTTCCTGGAGCCCCGAGCCAGCCCTGTCC CTCCCCAGTGCAGCATGGCACTCAGGAGATACAGGCTGGACATGGGGCAGTCGTTCTGGGGAGGCCTGGCCTAGCAGCCACCCACCTGAGCCCTCCCGGC CAGGCTTCGTGCTGGGGTGGGCCATGTGCCAGGACAGGAGGGTCCCGGCGGAAAGCCAGCCCCGGACTCATCGTGACATTGAGATCCCACTGGAGGGTAG GGGTGGTAATAAACTTCTCCAAACGATCGTTGTCATTTTAGACAG

BOK.F4 (forward primer)
BOK.R6 (reverse primer)
Single exon skipping

Frame 0

APSPASRQLGVARCPPRCPGPRRRGRSPPRARE APWTWRSRLGRGRGGRRGGARVLAGLNGRVEVVV GGEQILKPELHPGARAMRRERCGAPHPRRRHGG AAALLGLRRRDHGRL*PLAHRQGAGGPGQGAGP GVRARAAAARRPLLERARACRAGPGTPG*GVRGA AAPGR*AGDDPAQRLPQRGASAAHLPAV*ACGD RCVPGRGWPHLLCRHHVGQGGVPVCGGRGAGRG LCEAGPACHGPRPRGLPGGVRAQDPGNLAAETRR MD*CPQVCGQHRPWPPLPLAGGCTLQLRPLPEG CLLRAAAREMSCPPGSGRSLALWAQRRRPSAPE HIFLLPTRAWSTLTLGDPLSPVPPQTQALRKG*V GRGFPEPGAGLWGSLRPSPLVSLLL*SLCFPFS FWGQEVRVNSQASDAGAQNTCSHLSPR*RGPGT PALT*APGEGAREHLLSPEPQVKGPGTPALT*AP GEGARNTCSHLSPR*RGPGTPALT*APGEGARE HLLSPEPQVKGPRTPALT*APGEGARNTCSHLS PR*RGPGTPALT*APGEGARNTCSHLSPR*RGPE HLLSPEPPVKGPGTLALT*APGEGARNTSSHLS PR*RGPEHLLSPEPQVKGPGNTSHLNPGVPSQE EGPSQDMSPQGPCTFNLKVTLAWLKLEELWGLSL *TERKVHMLVA*SVSGGRV*HPLVMGSLCGGDR GRRAPGQHT*PMDVEPTAEKECCMSRIPVHCQW RVRVTPSAIFVLILIQPFGDVPIRAP*ELRCLGS PAPQVPPHTAFP*RLHF*AHVRHLSWSPEPALS LPSAAWHSGDTGWTWGSRSGEAWPSSHPPEPSR PGFVLGWAMCQDRRVPAESQPRTHRDIEIPLEGR GGNKLLQTIVVILD

Frame 1

PQAQPLASWESRAAHLAAQAPDAAAGAPQERGK PRGPGAPGSGVDGAGAGAGRASSRV*MEGSRSS SAASRS*SQNSTPAPAPCGGRGAAPPTRVAAMEV LRRSSVFAAEIMDAFDRSPTDKELVAQAKALGR EYVHARLLRAGLSWSAPERAAPVPGRLAEVCAV LLRLGDELEMIRPSVYRNVARQLHISLQSEPVVT DAFLAVAGHIFSAGITWGKVVSLYAVAAGLAVD CVRQAQPAMVHALVDCLGEFVRKTLATWLRRRG GWTDVLKCVVSTDPGLRSHWLVAALCSFGRFLKA AFFVLLPER*AAHLAVAAAWPSGPNAGGPQHPN TSSSSPPEPGAL*PSETP*APFLRRPRPSGRGE WGGAFLSLELGFGAACDPPRLCPFSCDLCVFPFL SGARKSGSTPRPQMQGPRTPALT*APGEGAREH LLSPEPQVKGPGNTCSHLNPR*RGPEHLLSPEP QVKGPGTPALT*APGEGAREHLLSPEPQVKGPGN TCSHLNPR*RGPEHLLSPEPQVKGPGTPALT*A PGEGAREHLLSPEPLVKGPGTLALT*APGEGAR NTCSHLSPR*RGPEHLLSPEPQVKGPGTPPLT*A PGEGARNTSCHLSPR*RGPGTPLT*TRGSHPRK KGHLRT*VLRGPAHSI*R*PWPG*SWKSCGDSA CKQSVRFTCWLLNPFLEEEYDTHL*WGPCAVGTG AGGLQASTPNPWMWNLRPRRNVA*VGSQSIVSG G*G*PHLLFLCSSSYNHLGMCLLGLRKNSDAWE AQPLRCPHTQPSLDAYISRHM*GIFPGAPSQPCP SPVQHGTQEIQAGHGAVVLGRPGLAATHLSPPG QASCWGGPCARTGGSRRKASPGLIVTLRSHWRV GVVINFSKRSLSF*T

Frame 2

PKPSLSPAGSRALPTSLPRPPTPRQEPPKSAGS PVDLALPARAWTGRAPGRGARPRGSEWKGRGRR RRRADPEARTPPRRPRHAAGEVRRPPPASPPWR CCGAPRSSPPRSWTPLTARPQTRSWWPRPRRWAG STCTRGCCAPASPGARPSVPRRSRDAWLRCARC CCAWAMSWR*SGPASTATWRVSCTSPCSLSLW* PMRSWPWLATSSLQASRGARWCPCMRWPRGWPWT V*GRPSLPWSTPSWTAWGSSCARPWQPGCGDAA DGLMSSSVWSAQTLASAPTGWWLHSAASAAS*R LPSSCCCQRDELPTWQWPQPGPLGPTQEALSTRT HLPPPHPSLEHSNPRRPPKPRSSADPGPPEGVS GEGLS*AWSWALGQPATLPACVPSPVISVFSLF FLGPGSQGQLPGLRCRGPEHLLSPEPQVKGPGNT CSHLSPR*RGPGTPALT*TPGEGARNTCSHLSP R*RGPEHLLSPEPQVKGPGNTCSHLSPR*RGPG TPALT*TPGEGAQNTCSHLSPR*RGPEHLLSPEP QVKGPGNTCSHLSPW*RGPEHLLSPEPQVKGPG TPALT*APGEGARNTCSHLSPR*RGPEHLLSPE PQVKGPGTPPVT*APGEGAREHLSPEPGGPIPGR RAISGHESSGALHIQSEGDPGLAEAGRAVGTQP VNRA*GSHAGCLIRFWRKSMTPTCDGVLVRWGP GPAGSRPAHLTHGCGTYGREGMLHESDPSPLSVE GEGDPICYFCAHPHTTIWGCAY*GSVRTQMPGK PSPSGAPTHSLPLTPTFLGTCEASFLEPRASPV PPQCSMALRRYRLDMGQSFWGGLA*QPPT*ALPA RLRAGVGHVPGQEGPGGKPAPDSS*H*DPTGG* GW**TSPNDRCHFRQ