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Sequences

Sequence BOK.fAug05

GCCCCAAGCCCAGCCTCTCGCCAGCTGGGAGTCGCGCGCTGCCCACCTCGCTGCCCAGGCCCCCGACGCCGCGGCAGGAGCCCCCCAAGAGCGCGGGAAG CCCCGTGGACCTGGCGCTCCCGGCTCGGGCGTGGACGGGGCGGGCGCCGGGGCGGGGCGCGCGTCCTCGCGGGTCTGAATGGAAGGGTCGAGGTCGTCGT CGGCGGCGAGCAGATCCTGAAGCCAGAACTCCACCCCGGCGCCCGCGCCATGCGGCGGGAGAGGCATCACGTGGGGCAAGGTGGTGTCCCTGTATGCGGT GGCCGCGGGGCTGGCCGTGGACTGTGTGAGGCAGGCCCAGCCTGCCATGGTCCACGCCCTCGTGGACTGCCTGGGGGAGTTCGTGCGCAAGACCCTGGCA ACCTGGCTGCGGAGACGCGGCGGATGGACTGATGTCCTCAAGTGTGTGGTCAGCACAGACCCTGGCCTCCGCTCCCACTGGCTGGTGGCTGCACTCTGCA GCTTCGGCCGCTTCCTGAAGGCTGCCTTCTTCGTGCTGCTGCCAGAGAGATGAGCTGCCCACCTGGCAGTGGCCGCAGCCTGGCCCTCTGGGCCCAACGC AGGAGGCCCTCAGCACCCGAACACATCTTCCTCCTCCCCACCCGAGCCTGGAGCACTCTAACCCTCGGAGACCCCCTAAGCCCCGTTCCTCCGCAGACCC AGGCCCTCCGGAAGGGGTGAGTGGGGAGGGGCTTTCCTGAGCCTGGAGCTGGGCTTTGGGGCAGCCTGCGACCCTCCCCGCTTGTGTCCCTTCTCCTGTG ATCTCTGTGTTTTCCCTTTTCTTTCTGGGGCCAGGAAGTCAGGGTCAACTCCCAGGCCTCAGATGCAGGGGCCCAGAACACCTGCTCTCACCTGAGCCCC AGGTGAAGGGGCCCGGGAACACCTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGGAACACCTGCTCTCACCTGAACCCCAGGTGAAGGGGCCCGGAA CACCTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGAACACCTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGGAACACCTGCTCTCACCTGAGC CCCAGGTGAAGGGGCCCAGAACACCTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGAACACCTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGG AACACCTGCTCTCACCTGAGCCCCTGGTGAAGGGGCCCGGAACACTTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGAACACCTGCTCTCACCTGAG CCCCCGGTGAAGGGGCCCGGAACACTTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGAACACCTCCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGG AACACCTCCTGTCACCTGAGCCCCAGGTGAAGGGGCCCGGGAACACCTCTCACCTGAACCCGGGGGTCCCATCCCAGGAAGAAGGGCCATCTCAGGACAT GAGTCCTCAGGGGCCCTGCACATTCAATCTGAAGGTGACCCTGGCCTGGCTGAAGCTGGAAGAGCTGTGGGGACTCAGCCTGTAAACAGAGCGTAAGGTT CACATGCTGGTTGCTTAATCCGTTTCTGGAGGAAGAGTATGACACCCACTTGTGATGGGGTCCTTGTGCGGTGGGGACCGGGGCCGGCGGGCTCCAGGCC AGCACACCTAACCCATGGATGTGGAACCTACGGCCGAGAAGGAATGTTGCATGAGTCGGATCCCAGTCCATTGTCAGTGGAGGGTGAGGGTGACCCCATC TGCTATTTTTGTGCTCATCCTCATACAACCATTTGGGGATGTGCCTATTAGGGCTCCGTAAGAACTCAGATGCCTGGGAAGCCCAGCCCCTCAGGTGCCC CCACACACAGCCTTCCCTTGACGCCTACATTTCTAGGCACATGTGAGGCATCTTTCCTGGAGCCCCGAGCCAGCCCTGTCCCTCCCCAGTGCAGCATGGC ACTCAGGAGATACAGGCTGGACATGGGGCAGTCGTTCTGGGGAGGCCTGGCCTAGCAGCCACCCACCTGAGCCCTCCCGGCCAGGCTTCGTGCTGGGGTG GGCCATGTGCCAGGACAGGAGGGTCCCGGCGGAAAGCCAGCCCCGGACTCATCGTGACATTGAGATCCCACTGGAGGGTAGGGGTGGTAATAAACTTCTC CAAACGATCGTTGTCATTTTAGACAGAA

BOK.F4 (forward primer)
BOK.R6 (reverse primer)
Multiple exon skipping

Frame 0

APSPASRQLGVARCPPRCPGPRRRGRSPPRARE APWTWRSRLGRGRGGRRGGARVLAGLNGRVEVVV GGEQILKPELHPGARAMRRERHHVGQGGVPVCG GRGAGRGLCEAGPACHGPRPRGLPGGVRAQDPG NLAAETRRMD*CPQVCGQHRPWPPLPLAGGCTLQ LRPLPEGCLLRAAAREMSCPPGSGRSLALWAQR RRPSAPEHIFLLPTRAWSTLTLGDPLSPVPPQT QALRKG*VGRGFPEPGAGLWGSLRPSPLVSLLL* SLCFPFSFWGQEVRVNSQASDAGAQNTCSHLSP R*RGPGTPALT*APGEGAREHLLSPEPQVKGPG TPALT*APGEGARNTCSHLSPR*RGPGTPALT*A PGEGAQNTCSHLSPR*RGPEHLLSPEPQVKGPG NTCSHLSPW*RGPEHLLSPEPQVKGPGTPALT* APGEGARNTCSHLSPR*RGPEHLLSPEPQVKGPG TPPVT*APGEGAREHLSPEPGGPIPGRRAISGH ESSGALHIQSEGDPGLAEAGRAVGTQPVNRA*G SHAGCLIRFWRKSMTPTCDGVLVRWGPGPAGSRP AHLTHGCGTYGREGMLHESDPSPLSVEGEGDPI CYFCAHPHTTIWGCAY*GSVRTQMPGKPSPSGA PTHSLPLTPTFLGTCEASFLEPRASPVPPQCSMA LRRYRLDMGQSFWGGLA*QPPT*ALPARLRAGV GHVPGQEGPGGKPAPDSS*H*DPTGG*GW**TS PNDRCHFRQ

Frame 1

PQAQPLASWESRAAHLAAQAPDAAAGAPQERGK PRGPGAPGSGVDGAGAGAGRASSRV*MEGSRSS SAASRS*SQNSTPAPAPCGGRGITWGKVVSLYAV AAGLAVDCVRQAQPAMVHALVDCLGEFVRKTLA TWLRRRGGWTDVLKCVVSTDPGLRSHWLVAALC SFGRFLKAAFFVLLPER*AAHLAVAAAWPSGPNA GGPQHPNTSSSSPPEPGAL*PSETP*APFLRRP RPSGRGEWGGAFLSLELGFGAACDPPRLCPFSC DLCVFPFLSGARKSGSTPRPQMQGPRTPALT*AP GEGAREHLLSPEPQVKGPGNTCSHLNPR*RGPE HLLSPEPQVKGPGTPALT*APGEGAREHLLSPE PQVKGPRTPALT*APGEGARNTCSHLSPR*RGPG TPALT*APGEGARNTCSHLSPR*RGPEHLLSPE PPVKGPGTLALT*APGEGARNTSSHLSPR*RGP EHLLSPEPQVKGPGNTSHLNPGVPSQEEGPSQDM SPQGPCTFNLKVTLAWLKLEELWGLSL*TERKV HMLVA*SVSGGRV*HPLVMGSLCGGDRGRRAPG QHT*PMDVEPTAEKECCMSRIPVHCQWRVRVTPS AIFVLILIQPFGDVPIRAP*ELRCLGSPAPQVP PHTAFP*RLHF*AHVRHLSWSPEPALSLPSAAW HSGDTGWTWGSRSGEAWPSSHPPEPSRPGFVLGW AMCQDRRVPAESQPRTHRDIEIPLEGRGGNKLL QTIVVILDR

Frame 2

PKPSLSPAGSRALPTSLPRPPTPRQEPPKSAGS PVDLALPARAWTGRAPGRGARPRGSEWKGRGRR RRRADPEARTPPRRPRHAAGEASRGARWCPCMR WPRGWPWTV*GRPSLPWSTPSWTAWGSSCARPWQ PGCGDAADGLMSSSVWSAQTLASAPTGWWLHSA ASAAS*RLPSSCCCQRDELPTWQWPQPGPLGPT QEALSTRTHLPPPHPSLEHSNPRRPPKPRSSADP GPPEGVSGEGLS*AWSWALGQPATLPACVPSPV ISVFSLFFLGPGSQGQLPGLRCRGPEHLLSPEP QVKGPGNTCSHLSPR*RGPGTPALT*TPGEGARN TCSHLSPR*RGPEHLLSPEPQVKGPGNTCSHLS PR*RGPEHLLSPEPQVKGPGTPALT*APGEGAR EHLLSPEPLVKGPGTLALT*APGEGARNTCSHLS PR*RGPEHLLSPEPQVKGPGTPPLT*APGEGAR NTSCHLSPR*RGPGTPLT*TRGSHPRKKGHLRT *VLRGPAHSI*R*PWPG*SWKSCGDSACKQSVRF TCWLLNPFLEEEYDTHL*WGPCAVGTGAGGLQA STPNPWMWNLRPRRNVA*VGSQSIVSGG*G*PH LLFLCSSSYNHLGMCLLGLRKNSDAWEAQPLRCP HTQPSLDAYISRHM*GIFPGAPSQPCPSPVQHG TQEIQAGHGAVVLGRPGLAATHLSPPGQASCWG GPCARTGGSRRKASPGLIVTLRSHWRVGVVINFS KRSLSF*TE

Sequence BOK.cAug05

GCCCCAAGCCCAGCCTCTCGCCAGCTGGGAGTCGCGCGCTGCCCACCTCGCTGCCCAGGCCCCCGACGCCGCGGCAGGAGCCCCCCAAGAGCGCGGGAAG CCCCGTGGACCTGGCGCTCCCGGCTCGGGCGTGGACGGGGCGGGCGCCGGGGCGGGGCGCGCGTCCTCGCGGGTCTGAATGGAAGGGTCGAGGTCGTCGT CGGCGGCGAGCAGATCCTGAAGCCAGAACTCCACCCCGGCGCCCGCGCCATGCGGCGGGAGAGGTGCGGCGCCCCCCACCCGCGTCGCCGCCATGGAGGT GCTGCGGCGCTCCTCGGTCTTCGCCGCCGAGATCATGGACGCCTTTGACCGCTCGCCCACAGACAAGGAGCTGGTGGCCCAGGCCAAGGCGCTGGGCCGG GAGTACGTGCACGCGCGGCTGCTGCGCGCCGGCCTCTCCTGGAGCGCGCCCGAGCGTGCCGCGCCGGTCCCGGGACGCCTGGCTGAGGTGTGCGCGGTGC TGCTGCGCCTGGATTCTTGCTGTTACTGGCCGAGATCCCGCCCGCTGCCCTCTGGATTCCGAGGCCTCCGAGGCCCCCCCATTGGAAACTGCGCCCACCG GGCTGGGTAGCCAAAACCTCAGTATTCGTTGGTGCTGTGAGGACATCTGGGGGTGCTCGGTTCTGGGGTCACACACGGTCCACTGGCTGCCCCCACCGTC TCCAAGGCGATGAGCTGGAGATGATCCGGCCCAGCGTCTACCGCAACGTGGCGCGTCAGCTGCACATCTCCCTGCAGTCTGAGCCTGTGGTGACCGATGC GTTCCTGGCCGTGGCTGGCCACATCTTCTCTGCAGGCATCACGTGGGGCAAGGTGGTGTCCCTGTATGCGGTGGCCGCGGGGCTGGCCGTGGACTGTGTG AGGCAGGCCCAGCCTGCCATGGTCCACGCCCTCGTGGACTGCCTGGGGGAGTTCGTGCGCAAGACCCTGGCAACCTGGCTGCGGAGACGCGGCGGATGGA CTGATGTCCTCAAGTGTGTGGTCAGCACAGACCCTGGCCTCCGCTCCCACTGGCTGGTGGCTGCACTCTGCAGCTTCGGCCGCTTCCTGAAGGCTGCCTT CTTCGTGCTGCTGCCAGAGAGATGAGCTGCCCACCTGGCAGTGGCCGCAGCCTGGCCCTCTGGGCCCAACGCAGGAGGCCCTCAGCACCCGAACACATCT TCCTCCTCCCCACCCGAGCCTGGAGCACTCTAACCCTCGGAGACCCCCTAAGCCCCGTTCCTCCGCAGACCCAGGCCCTCCGGAAGGGGTGAGTGGGGAG GGGCTTTCCTGAGCCTGGAGCTGGGCTTTGGGGCAGCCTGCGACCCTCCCCGCTTGTGTCCCTTCTCCTGTGATCTCTGTGTTTTCCCTTTTCTTTCTGG GGCCAGGAAGTCAGGGTCAACTCCCAGGCCTCAGATGCAGGGGCCCAGAACACCTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGGAACACCTGCTC TCACCTGAGCCCCAGGTGAAGGGGCCCGGGAACACCTGCTCTCACCTGAACCCCAGGTGAAGGGGCCCGGAACACCTGCTCTCACCTGAGCCCCAGGTGA AGGGGCCCGGAACACCTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGGAACACCTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGGAACACCTG CTCTCACCTGAACCCCAGGTGAAGGGGCCCAGAACACCTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGAACACCTGCTCTCACCTGAGCCCCAGGT GAAGGGGCCCGGGAACACCTGCTCTCACCTGAGCCCCTGGTGAAGGGGCCCGGAACACTTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGAACACCT GCTCTCACCTGAGCCCCCGGTGAAGGGGCCCGGAACACTTGCTCTCACCTGAGCCCCAGGTGAAGGGGCCCGGAACACCTCCTCTCACCTGAGCCCCAGG TGAAGGGGCCCGGAACACCTCCTGTCACCTGAGCCCCAGGTGAAGGGGCCCGGGAACACCTCTCACCTGAACCCGGGGGTCCCATCCCAGGAAGAAGGGC CATCTCAGGACATGAGTCCTCAGGGGCCCTGCACATTCAATCTGAAGGTGACCCTGGCCTGGCTGAAGCTGGAAGAGCTGTGGGGACTCAGCCTGTAAAC AGAGCGTAAGGTTCACATGCTGGTTGCTTAATCCGTTTCTGGAGGAAGAGTATGACACCCACTTGTGATGGGGTCCTTGTGCGGTGGGGACCGGGGCCGG CGGGCTCCAGGCCAGCACACCTAACCCATGGATGTGGAACCTACGGCCGAGAAGGAATGTTGCATGAGTCGGATCCCAGTCCATTGTCAGTGGAGGGTGA GGGTGACCCCATCTGCTATTTTTGTGCTCATCCTCATACAACCATTTGGGGATGTGCCTATTAGGGCTCCGTAAGAACTCAGATGCCTGGGAAGCCCAGC CCCTCAGGTGCCCCCACACACAGCCTTCCCTTGACGCCTACATTTCTAGGCACATGTGAGGCATCTTTCCTGGAGCCCCGAGCCAGCCCTGTCCCTCCCC AGTGCAGCATGGCACTCAGGAGATACAGGCTGGACATGGGGCAGTCGTTCTGGGGAGGCCTGGCCTAGCAGCCACCCACCTGAGCCCTCCCGGCCAGGCT TCGTGCTGGGGTGGGCCATGTGCCAGGACAGGAGGGTCCCGGCGGAAAGCCAGCCCCGGACTCATCGTGACATTGAGATCCCACTGGAGGGTAGGGGTGG TAATAAACTTCTCCAAACGATCGTTGTCATTTTAGACAG

BOK.F4 (forward primer)
BOK.R6 (reverse primer)
Multiple exon skipping

Frame 0

APSPASRQLGVARCPPRCPGPRRRGRSPPRARE APWTWRSRLGRGRGGRRGGARVLAGLNGRVEVVV GGEQILKPELHPGARAMRRERCGAPHPRRRHGG AAALLGLRRRDHGRL*PLAHRQGAGGPGQGAGP GVRARAAAARRPLLERARACRAGPGTPG*GVRGA AAPGFLLLLAEIPPAALWIPRPPRPPHWKLRPP GWVAKTSVFVGAVRTSGGARFWGHTRSTGCPHR LQGDELEMIRPSVYRNVARQLHISLQSEPVVTDA FLAVAGHIFSAGITWGKVVSLYAVAAGLAVDCV RQAQPAMVHALVDCLGEFVRKTLATWLRRRGGW TDVLKCVVSTDPGLRSHWLVAALCSFGRFLKAAF FVLLPER*AAHLAVAAAWPSGPNAGGPQHPNTS SSSPPEPGAL*PSETP*APFLRRPRPSGRGEWG GAFLSLELGFGAACDPPRLCPFSCDLCVFPFLSG ARKSGSTPRPQMQGPRTPALT*APGEGAREHLL SPEPQVKGPGNTCSHLNPR*RGPEHLLSPEPQV KGPGTPALT*APGEGAREHLLSPEPQVKGPGNTC SHLNPR*RGPEHLLSPEPQVKGPGTPALT*APG EGAREHLLSPEPLVKGPGTLALT*APGEGARNT CSHLSPR*RGPEHLLSPEPQVKGPGTPPLT*APG EGARNTSCHLSPR*RGPGTPLT*TRGSHPRKKG HLRT*VLRGPAHSI*R*PWPG*SWKSCGDSACK QSVRFTCWLLNPFLEEEYDTHL*WGPCAVGTGAG GLQASTPNPWMWNLRPRRNVA*VGSQSIVSGG* G*PHLLFLCSSSYNHLGMCLLGLRKNSDAWEAQ PLRCPHTQPSLDAYISRHM*GIFPGAPSQPCPSP VQHGTQEIQAGHGAVVLGRPGLAATHLSPPGQA SCWGGPCARTGGSRRKASPGLIVTLRSHWRVGV VINFSKRSLSF*T

Frame 1

PQAQPLASWESRAAHLAAQAPDAAAGAPQERGK PRGPGAPGSGVDGAGAGAGRASSRV*MEGSRSS SAASRS*SQNSTPAPAPCGGRGAAPPTRVAAMEV LRRSSVFAAEIMDAFDRSPTDKELVAQAKALGR EYVHARLLRAGLSWSAPERAAPVPGRLAEVCAV LLRLDSCCYWPRSRPLPSGFRGLRGPPIGNCAHR AG*PKPQYSLVL*GHLGVLGSGVTHGPLAAPTV SKAMSWR*SGPASTATWRVSCTSPCSLSLW*PM RSWPWLATSSLQASRGARWCPCMRWPRGWPWTV* GRPSLPWSTPSWTAWGSSCARPWQPGCGDAADG LMSSSVWSAQTLASAPTGWWLHSAASAAS*RLP SSCCCQRDELPTWQWPQPGPLGPTQEALSTRTHL PPPHPSLEHSNPRRPPKPRSSADPGPPEGVSGE GLS*AWSWALGQPATLPACVPSPVISVFSLFFL GPGSQGQLPGLRCRGPEHLLSPEPQVKGPGNTCS HLSPR*RGPGTPALT*TPGEGARNTCSHLSPR* RGPEHLLSPEPQVKGPGNTCSHLSPR*RGPGTP ALT*TPGEGAQNTCSHLSPR*RGPEHLLSPEPQV KGPGNTCSHLSPW*RGPEHLLSPEPQVKGPGTP ALT*APGEGARNTCSHLSPR*RGPEHLLSPEPQ VKGPGTPPVT*APGEGAREHLSPEPGGPIPGRRA ISGHESSGALHIQSEGDPGLAEAGRAVGTQPVN RA*GSHAGCLIRFWRKSMTPTCDGVLVRWGPGP AGSRPAHLTHGCGTYGREGMLHESDPSPLSVEGE GDPICYFCAHPHTTIWGCAY*GSVRTQMPGKPS PSGAPTHSLPLTPTFLGTCEASFLEPRASPVPP QCSMALRRYRLDMGQSFWGGLA*QPPT*ALPARL RAGVGHVPGQEGPGGKPAPDSS*H*DPTGG*GW **TSPNDRCHFRQ

Frame 2

PKPSLSPAGSRALPTSLPRPPTPRQEPPKSAGS PVDLALPARAWTGRAPGRGARPRGSEWKGRGRR RRRADPEARTPPRRPRHAAGEVRRPPPASPPWR CCGAPRSSPPRSWTPLTARPQTRSWWPRPRRWAG STCTRGCCAPASPGARPSVPRRSRDAWLRCARC CCAWILAVTGRDPARCPLDSEASEAPPLETAPT GLGSQNLSIRWCCEDIWGCSVLGSHTVHWLPPPS PRR*AGDDPAQRLPQRGASAAHLPAV*ACGDRC VPGRGWPHLLCRHHVGQGGVPVCGGRGAGRGLC EAGPACHGPRPRGLPGGVRAQDPGNLAAETRRMD *CPQVCGQHRPWPPLPLAGGCTLQLRPLPEGCL LRAAAREMSCPPGSGRSLALWAQRRRPSAPEHI FLLPTRAWSTLTLGDPLSPVPPQTQALRKG*VGR GFPEPGAGLWGSLRPSPLVSLLL*SLCFPFSFW GQEVRVNSQASDAGAQNTCSHLSPR*RGPGTPA LT*APGEGAREHLLSPEPQVKGPGTPALT*APGE GARNTCSHLSPR*RGPGTPALT*APGEGAREHL LSPEPQVKGPRTPALT*APGEGARNTCSHLSPR *RGPGTPALT*APGEGARNTCSHLSPR*RGPEHL LSPEPPVKGPGTLALT*APGEGARNTSSHLSPR *RGPEHLLSPEPQVKGPGNTSHLNPGVPSQEEG PSQDMSPQGPCTFNLKVTLAWLKLEELWGLSL*T ERKVHMLVA*SVSGGRV*HPLVMGSLCGGDRGR RAPGQHT*PMDVEPTAEKECCMSRIPVHCQWRV RVTPSAIFVLILIQPFGDVPIRAP*ELRCLGSPA PQVPPHTAFP*RLHF*AHVRHLSWSPEPALSLP SAAWHSGDTGWTWGSRSGEAWPSSHPPEPSRPG FVLGWAMCQDRRVPAESQPRTHRDIEIPLEGRGG NKLLQTIVVILD