Sequences - Rnomics 📊 Palace

        ☰
    
        Rnomics 📊 Palace
    
                       Back to Rnomics Platform
            
                       Open Session

1 warning:

Access restricted to public data.
Please sign in at Rnomics Platform to access your data.

Navigation

Home

Sequences

Sequence PIK3CD.bAug05

CTCGCGCCCAGCGCAGTCGCTCCGAGCGGCCGCGAGCAGAGCCGCCCAGCCCTGCCAGCTGCGCCGGGACGATAAGGAGTCAGGCCAGGGCGGGATGACA CTCATTGATTCTAAAGCATCTTTAATCTGCCAGGCGGAGGGGGCTTTGCTGGTCTTTCTTGGACTATTCCAGAGAGGACAACTGTCATCTGGGAAGTAAC AACGCAGGATGCCCCCTGGGGTGGACTGCCCCATGGAATTCTGGACCAAGGAGGAGAATCAGAGCGTTGTGGTTGACTTCCTGCTGCCCACAGGGGTCTA CCTGAACTTCCCTGTGTCCCGCAATGCCAACCTCAGCACCATCAAGCAGCTGCTGTGGCACCGCGCCCAGTATGAGCCGCTCTTCCACATGCTCAGTGGC CCCGAGGCCTATGTGTTCACCTGCATCAACCAGACAGCGGAGCAGCAAGAGCTGGAGGACGAGCAACGGCGTCTGTGTGACGTGCAGCCCTTCCTGCCCG TCCTGCGCCTGGTGGCCCGTGAGGGCGACCGCGTGAAGAAGCTCATCAACTCACAGATCAGCCTCCTCATCGGCAAAGGCCTCCACGAGTTTGACTCCTT GTGCGACCCAGAAGTGAACGACTTTCGCGCCAAGATGTGCCAATTCTGCGAGGAGGCGGCCGCCCGCCGGCAGCAGCTGGGCTGGGAGGCCTGGCTGCAG TACAGTTTCCCCCTGCAGCTGGAGCCCTCGGCTCAAACCTGGGGGCCTGGTACCCTGCGGCTCCCGAACCGGGCCCTTCTGGTCAACGTTAAGTTTGAGG GCAGCGAGGAGAGCTTCACCTTCCAGGTGTCCACCAAGGACGTGCCGCTGGCGCTGATGGCCTGTGCCCTGCGGAAGAAGGCCACAGTGTTCCGGCAGCC GCTGGTGGAGCAGCCGGAAGACTACACGCTGCAGGTGAACGGCAGGCATGAGTACCTGTATGGCAGCTACCCGCTCTGCCAGTTCCAGTACATCTGCAGC TGCCTGCACAGTGGGTTGACCCCTCACCTGACCATGGTCCATTCCTCCTCCATCCTCGCCATGCGGGATGAGCAGAGCAACCCTGCCCCCCAGGTCCAGA AACCGCGTGCCAAACCACCTCCCATTCCTGCGAAGAAGCCTTCCTCTGTGTCCCTGTGGTCCCTGGAGCAGCCGTTCCGCATCGAGCTCATCCAGGGCAG CAAAGTGAACGCCGACGAGCGGATGAAGCTGGTGGTGCAGGCCGGGCTTTTCCACGGCAACGAGATGCTGTGCAAGACGGTGTCCAGCTCGGAGGTGAGC GTGTGCTCGGAGCCCGTGTGGAAGCAGCGGCTGGAGTTCGACATCAACATCTGCGACCTGCCCCGCATGGCCCGTCTCTGCTTTGCGCTGTACGCCGTGA TCGAGAAAGCCAAGAAGGCTCGCTCCACCAAGAAGAAGTCCAAGAAGGCGGACTGCCCCATTGCCTGGGCCAACCTCATGCTGTTTGACTACAAGGACCA GCTTAAGACCGGGGAACGCTGCCTCTACATGTGGCCCTCCGTCCCAGATGAGAAGGGCGAGCTGCTGAACCCCACGGGCACTGTGCGCAGTAACCCCAAC ACGGATAGCGCCGCTGCCCTGCTCATCTGCCTGCCCGAGGTGGCCCCGCACCCCGTGTACTACCCCGCCCTGGAGAAGATCTTGGAGCTGGGGCGACACA GCGAGTGTGTGCATGTCACCGAGGAGGAGCAGCTGCAGCTGCGGGAAATCCTGGAGCGGCGGGGGTCTGGGGAGCTGTATGAGCACGAGAAGGACCTGGT GTGGAAGCTGCGGCATGAAGTCCAGGAGCACTTCCCGGAGGCGCTAGCCCGGCTGCTGCTGGTCACCAAGTGGAACAAGCATGAGGATGTGGCCCAGATG CTCTACCTGCTGTGCTCCTGGCCGGAGCTGCCCGTCCTGAGCGCCCTGGAGCTGCTAGACTTCAGCTTCCCCGATTGCCACGTAGGCTCCTTCGCCATCA AGTCGCTGCGGAAACTGACGGACGATGAGCTGTTCCAGTACCTGCTGCAGCTGGTGCAGGTGCTCAAGTACGAGTCCTACCTGGACTGCGAGCTGACCAA ATTCCTGCTGGACCGGGCCCTGGCCAACCGCAAGATCGGCCACTTCCTTTTCTGGCACCTCCGCTCCGAGATGCACGTGCCGTCGGTGGCCCTGCGCTTC GGCCTCATCCTGGAGGCCTACTGCAGGGGCAGCACCCACCACATGAAGGTGCTGATGAAGCAGGGGGAAGCACTGAGCAAACTGAAGGCCCTGAATGACT TCGTCAAGCTGAGCTCTCAGAAGACCCCCAAGCCCCAGACCAAGGAGCTGATGCACTTGTGCATGCGGCAGGAGGCCTACCTAGAGGCCCTCTCCCACCT GCAGTCCCCACTCGACCCCAGCACCCTGCTGGCTGAAGTCTGCGTGGAGCAGTGCACCTTCATGGACTCCAAGATGAAGCCCCTGTGGATCATGTACAGC AACGAGGAGGCAGGCAGCGGCGGCAGCGTGGGCATCATCTTTAAGAACGGGGATGACCTCCGGCAGGACATGCTGACCCTGCAGATGATCCAGCTCATGG ACGTCCTGTGGAAGCAGGAGGGGCTGGACCTGAGGATGACCCCCTATGGCTGCCTCCCCACCGGGGACCGCACAGGCCTCATTGAGGTGGTACTCCGTTC AGACACCATCGCCAACATCCAACTCAACAAGAGCAACATGGCAGCCACAGCCGCCTTCAACAAGGATGCCCTGCTCAACTGGCTGAAGTCCAAGAACCCG GGGGAGGCCCTGGATCGAGCCATTGAGGAGTTCACCCTCTCCTGTGCTGGCTATTGTGTGGCCACATATGTGCTGGGCATTGGCGATCGGCACAGCGACA ACATCATGATCCGAGAGAGTGGGCAGCTGTTCCACATTGATTTTGGCCACTTTCTGGGGAATTTCAAGACCAAGTTTGGAATCAACCGCGAGCGTGTCCC ATTCATCCTCACCTACGACTTTGTCCATGTGATTCAGCAGGGGAAGACTAATAATAGTGAGAAATTTGAACGGTTCCGGGGCTACTGTGAAAGGGCCTAC ACCATCCTGCGGCGCCACGGGCTTCTCTTCCTCCACCTCTTTGCCCTGATGCGGGCGGCAGGCCTGCCTGAGCTCAGCTGCTCCAAAGACATCCAGTATC TCAAGGACTCCCTGGCACTGGGGAAAACAGAGGAGGAGGCACTGAAGCACTTCCGAGTGAAGTTTAACGAAGCCCTCCGTGAGAGCTGGAAAACCAAAGT GAACTGGCTGGCCCACAACGTGTCCAAAGACAACAGGCAGTAGTGGCTCCTCCCAGCCCTGGGCCCAAGAGGAGGCGGCTGCGGGTCGTGGGGACCAAGC ACATTGGTCCTAAAGGGGCTGAAGAGCCTGAACTGCACCTAACGGGAAAGAACCGACATGGCTGCCTTTTGTTTACACTGGTTATTTATTTATGACTTGA AATAGTTTAAGGAGCTAAACAGCCATAAACGGAAACGCCTCCTTCATGCAGCGGCGGTGCTGGGCCCCCCGAGGCTGCACCTGGCTCTCGGCTGAGGATT GTCACCCCAAGTCTTCCAGCTGGTGGATCTGGGCCCAGCAAAGACTGTTCTCCTCCCGAGGGAACCTTCTTCCCAGGCCTCCCGCCAGACTGCCTGGGTC CTGGCGCCTGGCGGTCACCTGGTGCCTACTGTCCGACAGGATGCCTTGATCCTCGTGCGACCCACCCTGTGTATCCTCCCTAGACTGAGTTCTGGCAGCT CCCCGAGGCAGCCGGGGTACCCTCTAGATTCAGGGATGCTTGCTCTCCACTTTTCAAGTGGGTCTTGGGTACGAGAATTCCCTCATCTTTCTCTACTGTA AAGTGATTTTGTTTGCAGGTAAGAAAATAATAGATGACTCACCACACCTCTACGGCTGGGGAGATCAGGCCCAGCCCCATAAAGGAGAATCTACGCTGGT CCTCAGGACGTGTTAAAGAGATCTGGGCCTCATGTAGCTCACCCCGGTCACGCATGAAGGCAAAAGCAGGTCAGAAGCGAATACTCTGCCATTATCTCAA AAATCTTTTTTTTTTTTTTGAGATGGGGTCTTCCTCTGTTGCCCAGGCTGGAGTGCAGTGGTGCAATCTTGGCTCACTGTAACCTCCGCCTCCCAGGTTC AAGTGATTCTTCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGTGTGCACCACCGTACCCAGCTAATTTTTGTATTTTAGTAGAGACGGGGGTTTCAC CATGTTGGCTGGGCTGGTCTCGAACTCCTGACCTCAGGTGATCCACCCGCCTGAGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACCGCGCCCGGC CCACTCTGCCATTGTCTAAGCCACCTCTGAAAGCAGGTTTTAACAAAAGGATGAGGCCAGAACTCTTCCAGAACCATCACCTTTGGGAACCTGCTGTGAG AGTGCTGAGGTACCAGAAGTGTGAGAACGAGGGGGCGTGCTGGGATCTTTCTCTCTGACTATACTTAGTTTGAAATGGTGCAGGCTTAGTCTTAAGCCTC CAAAGGCCTGGATTTGAGCAGCTTTAGAAATGCAGGTTCTAGGGCTTCTCCCAGCCTTCAGAAGCCAACTAACTCTGCAGATGGGGCTAGGACTGTGGGC TTTTAGCAGCCCACAGGTGATCCTAACATATCAGGCCATGGACTCAGGACCTGCCCGGTGATGCTGTTGATTTCTCAAAGGTCTTCCAAAACTCAACAGA GCCAGAAGTAGCCGCCCGCTCAGCGGCTCAGGTGCCAGCTCTGTTCTGATTCACCAGGGGTCCGTCAGTAGTCATTGCCACCCGCGGGGCACCTCCCTGG CCACACGCCTGTTCCCAGCAAGTGCTGAAACTCACTAGACCGTCTGCCTGTTTCGAAATGGGGAAAGCCGTGCGTGCGCGTTATTTATTTAAGTGCGCCT GTGTGCGCGGGTGTGGGAGCACACTTTGCAAAGCCACAGCGTTTCTGGTTTTGGGTGTACAGTCTTGTGTGCCTGGCGAGAAGAATATTTTCTATTTTTT TAAGTCATTTCATGTTTCTGTCTGGGGAAGGCAAGTTAGTTAAGTATCACTGATGTGGGTTGAGACCAGCACTCTGTGAAACCTTGAAATGAGAAGTAAA GGCAGATGAAAAGAAAGAAAAAGCCTTTTTATGTTCTTTTATGTTCTCGGCTCAAAAAGAAACAAGGGAGTGTAGGTTTAAAACCAAAACAGGAGAGAAG ACAAACCCCGCTCCGGCTGGAGTTAGTTAGAACCAGAACTTTATTGTAGCGGATACACTTTCTGACCTATCATGAGTATACACATCTGCGAAGGGAAACC GCGCGGCGACAGCGTGAGGACATCCCCTGGGCGTGAGCGTCTGTCCGCTGTCTAAACAGAGCAGCTACAGGGACGGGACATGGAGGATGGCCACACATAG CACAGCCACCAGTGTCCTCAGAACTAGCAGTCAGGGTCACAGAACAGTATTCAAAATGATTGCCCACCTGTTTTAGAAATCTAAAATTTTACATGTAACT AAGAGCAAAGTGCTATGTGGGTTTTAGACCATGACTGTTTGTTTGCTCTCCTGCCCTACCACCAAGCAAAGCAGCAGGGCTCCTGGGGGAGAGGGATTTC AACCCCCCTGATGGCAGGGGGTGCTCTGGGGAGGAGAGAGGAGAGAACAGGCTGTTTTGGAAAATTCCAGCACTTTGACTTCGGGCCATGCGTCTCTCCT GGACGTTCTGAGTACGGATCGCTCAGGCCTCCTGCCGTCTCCAAAAGGAGTGGTCAGCCGGTCTGCAGGACACCTCTCAGTTTCTCCTTTTACCAGCAGG AAAATGTACATTTAAGCATAAACCACAAGGCTGCACACGGTGAGACCAGGCCACTGCCCAGGGAGCTGCTGTCGGAGGAGGAGTGTGGAAAAGGGGCCAG GGACCCCGCACCCCACACCAAATTAGGAACAGGTAGAATCCCAAAGGCAAGGCCCGGCCACTTGTAGCTACCCTGATCCAGTCTCTTGAGCAGCACAGCT AGAGGCCTGTGGCCCCCGACTCCCAGCCATACTCAGACCTGAGCAGGGGCTGGGGCGGGGCTGGGCGGGGTGGGCCTCTCCCTCTGGGGTCTGGGGAGGG AGGACCCGGCGGGAAGAGCCCGTGCTCAGAGCTCCGAACTTCACTTTACATTCCAGTCCCGGGTTACACTAGCACAAAGGACACAAGGAATCCGATTTAT TTACAAAATATTAAAAAGTCAGTTAATCATCTACACAGTACCCCCCATCCTGCCATTATTTATACATGCACTAGTTTGGAAAAAATAAAAACTTTTTTTA AAAAAAGAAAAAAATGATTACCGGGTGGGAGTGAGCATGTTTTACCCTTTGTCAACGAGCCCAGCTGGCATGGCTTTTCTGGAGTGCGAGGGGCCAGTGT CCTCTCCCCGGGGGGAGACTCCAGACACAAACGCGGGGCCTGAGGCGCTGGGAGGCCCCTGTGCGAGCCGGATGGCGGCCAGAGAGGACGTGTCAGCTCC TCGTGGGACTGAAGAGCGCGACCAGGCAGAGGGTGGGCGGGGAGCCTAGGGTCCTACACACCAAGCACAGCGACGATCGTGGCGGGGAGGGGTCTGCACA CCTACTGGCCGAAATGACTTTGTACATCGTGGACCCTTGGGACTGGGAGAACGGATGGCAGCAGAGTCTTCGAAAGCAGAAACCGAGGTGGCCGGGGGCA CGGGTCAGTAGCTGAGGGTGGAGTCATCCCACTCCAGCTGCTGCTGCCGGGTTGCGTTCTGGGGGTCGCCCTGCTCCCCCTCCTCCTCCTCGCTGCTCTC CGACTCGGCGCTGGTGATGTCATCCTCTTCTTCGCCGTCCTCGCTTTCCTCTTCCTCTTCCTCTTCCTCCTCCTCCTCACTGCTGTGCTGGTCCTCATAG G

PIK3CD.F1 (forward primer)
PIK3CD.R1 (reverse primer)
Mutually exclusive exon skipping

Frame 0

LAPSAVAPSGREQSRPALPAAPGR*GVRPGRDD TH*F*SIFNLPGGGGFAGLSWTIPERTTVIWEVT TQDAPWGGLPHGILDQGGESERCG*LPAAHRGL PELPCVPQCQPQHHQAAAVAPRPV*AALPHAQW PRGLCVHLHQPDSGAARAGGRATASV*RAALPAR PAPGGP*GRPREEAHQLTDQPPHRQRPPRV*LL VRPRSERLSRQDVPILRGGGRPPAAAGLGGLAA VQFPPAAGALGSNLGAWYPAAPEPGPSGQR*V*G QRGELHLPGVHQGRAAGADGLCPAEEGHSVPAA AGGAAGRLHAAGERQA*VPVWQLPALPVPVHLQ LPAQWVDPSPDHGPFLLHPRHAG*AEQPCPPGPE TACQTTSHSCEEAFLCVPVVPGAAVPHRAHPGQ QSERRRADEAGGAGRAFPRQRDAVQDGVQLGGE RVLGARVEAAAGVRHQHLRPAPHGPSLLCAVRRD RESQEGSLHQEEVQEGGLPHCLGQPHAV*LQGP A*DRGTLPLHVALRPR*EGRAAEPHGHCAQ*PQ HG*RRCPAHLPARGGPAPRVLPRPGEDLGAGATQ RVCACHRGGAAAAAGNPGAAGVWGAV*AREGPG VEAAA*SPGALPGGASPAAAGHQVEQA*GCGPD ALPAVLLAGAARPERPGAARLQLPRLPRRLLRHQ VAAETDGR*AVPVPAAAGAGAQVRVLPGLRADQ IPAGPGPGQPQDRPLPFLAPPLRDARAVGGPAL RPHPGGLLQGQHPPHEGADEAGGSTEQTEGPE*L RQAELSEDPQAPDQGADALVHAAGGLPRGPLPP AVPTRPQHPAG*SLRGAVHLHGLQDEAPVDHVQ QRGGRQRRQRGHHL*ERG*PPAGHADPADDPAHG RPVEAGGAGPEDDPLWLPPHRGPHRPH*GGTPF RHHRQHPTQQEQHGSHSRLQQGCPAQLAEVQEP GGGPGSSH*GVHPLLCWLLCGHICAGHWRSAQRQ HHDPREWAAVPH*FWPLSGEFQDQVWNQPRACP IHPHLRLCPCDSAGED****EI*TVPGLL*KGL HHPAAPRASLPPPLCPDAGGRPA*AQLLQRHPVS QGLPGTGENRGGGTEALPSEV*RSPP*ELENQS ELAGPQRVQRQQAVVAPPSPGPKRRRLRVVGTK HIGPKGAEEPELHLTGKNRHGCLLFTLVIYL*LE IV*GAKQP*TETPPSCSGGAGPPEAAPGSRLRI VTPSLPAGGSGPSKDCSPPEGTFFPGLPPDCLG PGAWRSPGAYCPTGCLDPRATHPVYPP*TEFWQL PEAAGVPSRFRDACSPLFKWVLGTRIPSSFSTV K*FCLQVRK**MTHHTSTAGEIRPSPIKENLRW SSGRVKEIWASCSSPRSRMKAKAGQKRILCHYLK NLFFFFEMGSSSVAQAGVQWCNLGSL*PPPPRF K*FFCLSLLSSWDYRCAPPYPANFCILVETGVS PCWLGWSRTPDLR*STRLSLPKCWDYRHEPPRPA HSAIV*ATSESRF*QKDEARTLPEPSPLGTCCE SAEVPEV*ERGGVLGSFSLTILSLKWCRLSLKP PKAWI*AALEMQVLGLLPAFRSQLTLQMGLGLWA FSSPQVILTYQAMDSGPAR*CC*FLKGLPKLNR ARSSRPLSGSGASSVLIHQGSVSSHCHPRGTSL ATRLFPASAETH*TVCLFRNGESRACALFI*VRL CARVWEHTLQSHSVSGFGCTVLCAWREEYFLFF *VISCFCLGKAS*LSITDVG*DQHSVKP*NEK* RQMKRKKKPFYVLLCSRLKKKQGSVGLKPKQERR QTPLRLELVRTRTLL*RIHFLTYHEYTHLRRET ARRQREDIPWA*ASVRCLNRAATGTGHGGWPHI AQPPVSSELAVRVTEQYSK*LPTCFRNLKFYM*L RAKCYVGFRP*LFVCSPALPPSKAAGLLGERDF NPPDGRGCSGEERGENRLFWKIPAL*LRAMRLS WTF*VRIAQASCRLQKEWSAGLQDTSQFLLLPAG KCTFKHKPQGCTR*DQATAQGAAVGGGVWKRGQ GPRTPHQIRNR*NPKGKARPLVATLIQSLEQHS *RPVAPDSQPYSDLSRGWGGAGRGGPLPLGSGEG GPGGKSPCSELRTSLYIPVPGYTSTKDTRNPIY LQNIKKSVNHLHSTPHPAIIYTCTSLEKIKTFF KKRKK*LPGGSEHVLPFVNEPSWHGFSGVRGASV LSPGGDSRHKRGA*GAGRPLCEPDGGQRGRVSS SWD*RARPGRGWAGSLGSYTPSTATIVAGRGLH TYWPK*LCTSWTLGTGRTDGSRVFESRNRGGRGH GSVAEGGVIPLQLLLPGCVLGVALLPLLLLAAL RLGAGDVILFFAVLAFLFLFLFLLLLTAVLVLI

Frame 1

SRPAQSLRAAASRAAQPCQLRRDDKESGQGGMT LIDSKASLICQAEGALLVFLGLFQRGQLSSGK* QRRMPPGVDCPMEFWTKEENQSVVVDFLLPTGVY LNFPVSRNANLSTIKQLLWHRAQYEPLFHMLSG PEAYVFTCINQTAEQQELEDEQRRLCDVQPFLP VLRLVAREGDRVKKLINSQISLLIGKGLHEFDSL CDPEVNDFRAKMCQFCEEAAARRQQLGWEAWLQ YSFPLQLEPSAQTWGPGTLRLPNRALLVNVKFE GSEESFTFQVSTKDVPLALMACALRKKATVFRQP LVEQPEDYTLQVNGRHEYLYGSYPLCQFQYICS CLHSGLTPHLTMVHSSSILAMRDEQSNPAPQVQ KPRAKPPPIPAKKPSSVSLWSLEQPFRIELIQGS KVNADERMKLVVQAGLFHGNEMLCKTVSSSEVS VCSEPVWKQRLEFDINICDLPRMARLCFALYAV IEKAKKARSTKKKSKKADCPIAWANLMLFDYKDQ LKTGERCLYMWPSVPDEKGELLNPTGTVRSNPN TDSAAALLICLPEVAPHPVYYPALEKILELGRH SECVHVTEEEQLQLREILERRGSGELYEHEKDLV WKLRHEVQEHFPEALARLLLVTKWNKHEDVAQM LYLLCSWPELPVLSALELLDFSFPDCHVGSFAI KSLRKLTDDELFQYLLQLVQVLKYESYLDCELTK FLLDRALANRKIGHFLFWHLRSEMHVPSVALRF GLILEAYCRGSTHHMKVLMKQGEALSKLKALND FVKLSSQKTPKPQTKELMHLCMRQEAYLEALSHL QSPLDPSTLLAEVCVEQCTFMDSKMKPLWIMYS NEEAGSGGSVGIIFKNGDDLRQDMLTLQMIQLM DVLWKQEGLDLRMTPYGCLPTGDRTGLIEVVLRS DTIANIQLNKSNMAATAAFNKDALLNWLKSKNP GEALDRAIEEFTLSCAGYCVATYVLGIGDRHSD NIMIRESGQLFHIDFGHFLGNFKTKFGINRERVP FILTYDFVHVIQQGKTNNSEKFERFRGYCERAY TILRRHGLLFLHLFALMRAAGLPELSCSKDIQY LKDSLALGKTEEEALKHFRVKFNEALRESWKTKV NWLAHNVSKDNRQ*WLLPALGPRGGGCGSWGPS TLVLKGLKSLNCT*RERTDMAAFCLHWLFIYDL K*FKELNSHKRKRLLHAAAVLGPPRLHLALG*GL SPQVFQLVDLGPAKTVLLPREPSSQASRQTAWV LAPGGHLVPTVRQDALILVRPTLCILPRLSSGS SPRQPGYPLDSGMLALHFSSGSWVREFPHLSLL* SDFVCR*ENNR*LTTPLRLGRSGPAP*RRIYAG PQDVLKRSGPHVAHPGHA*RQKQVRSEYSAIIS KIFFFFLRWGLPLLPRLECSGAILAHCNLRLPGS SDSSASAS*VAGITGVHHRTQLIFVF**RRGFH HVGWAGLELLTSGDPPA*ASQSAGITGMSHRAR PTLPLSKPPLKAGFNKRMRPELFQNHHLWEPAVR VLRYQKCENEGACWDLSL*LYLV*NGAGLVLSL QRPGFEQL*KCRF*GFSQPSEAN*LCRWG*DCG LLAAHR*S*HIRPWTQDLPGDAVDFSKVFQNSTE PEVAARSAAQVPALF*FTRGPSVVIATRGAPPW PHACSQQVLKLTRPSACFEMGKAVRARYLFKCA CVRGCGSTLCKATAFLVLGVQSCVPGEKNIFYFF KSFHVSVWGRQVS*VSLMWVETSTL*NLEMRSK GR*KERKSLFMFFYVLGSKRNKGV*V*NQNRRE DKPRSGWS*LEPELYCSGYTF*PIMSIHICEGKP RGDSVRTSPGRERLSAV*TEQLQGRDMEDGHT* HSHQCPQN*QSGSQNSIQNDCPPVLEI*NFTCN *EQSAMWVLDHDCLFALLPYHQAKQQGSWGRGIS TPLMAGGALGRREERTGCFGKFQHFDFGPCVSP GRSEYGSLRPPAVSKRSGQPVCRTPLSFSFYQQ ENVHLSINHKAAHGETRPLPRELLSEEECGKGAR DPAPHTKLGTGRIPKARPGHL*LP*SSLLSSTA RGLWPPTPSHTQT*AGAGAGLGGVGLSLWGLGR EDPAGRARAQSSELHFTFQSRVTLAQRTQGIRFI YKILKSQLIIYTVPPILPLFIHALVWKK*KLFL KKEKNDYRVGVSMFYPLSTSPAGMAFLECEGPV SSPRGETPDTNAGPEALGGPCASRMAAREDVSAP RGTEERDQAEGGRGA*GPTHQAQRRSWRGGVCT PTGRNDFVHRGPLGLGERMAAESSKAETEVAGG TGQ*LRVESSHSSCCCRVAFWGSPCSPSSSSLLS DSALVMSSSSSPSSLSSSSSSSSSSSLLCWSS*

Frame 2

RAQRSRSERPRAEPPSPASCAGTIRSQARAG*H SLILKHL*SARRRGLCWSFLDYSREDNCHLGSN NAGCPLGWTAPWNSGPRRRIRALWLTSCCPQGS T*TSLCPAMPTSAPSSSCCGTAPSMSRSSTCSVA PRPMCSPASTRQRSSKSWRTSNGVCVTCSPSCP SCAWWPVRATA*RSSSTHRSASSSAKASTSLTP CATQK*TTFAPRCANSARRRPPAGSSWAGRPGCS TVSPCSWSPRLKPGGLVPCGSRTGPFWSTLSLR AARRASPSRCPPRTCRWR*WPVPCGRRPQCSGS RWWSSRKTTRCR*TAGMSTCMAATRSASSSTSAA ACTVG*PLT*PWSIPPPSSPCGMSRATLPPRSR NRVPNHLPFLRRSLPLCPCGPWSSRSASSSSRA AK*TPTSG*SWWCRPGFSTATRCCARRCPARR*A CARSPCGSSGWSSTSTSATCPAWPVSALRCTP* SRKPRRLAPPRRSPRRRTAPLPGPTSCCLTTRT SLRPGNAASTCGPPSQMRRASC*TPRALCAVTPT RIAPLPCSSACPRWPRTPCTTPPWRRSWSWGDT ASVCMSPRRSSCSCGKSWSGGGLGSCMSTRRTW CGSCGMKSRSTSRRR*PGCCWSPSGTSMRMWPRC STCCAPGRSCPS*APWSC*TSASPIAT*APSPS SRCGN*RTMSCSSTCCSWCRCSSTSPTWTAS*P NSCWTGPWPTARSATSFSGTSAPRCTCRRWPCAS ASSWRPTAGAAPTT*RC**SRGKH*AN*RP*MT SSS*ALRRPPSPRPRS*CTCACGRRPT*RPSPT CSPHSTPAPCWLKSAWSSAPSWTPR*SPCGSCTA TRRQAAAAAWASSLRTGMTSGRTC*PCR*SSSW TSCGSRRGWT*G*PPMAASPPGTAQASLRWYSV QTPSPTSNSTRATWQPQPPSTRMPCSTG*SPRTR GRPWIEPLRSSPSPVLAIVWPHMCWALAIGTAT TS*SERVGSCSTLILATFWGISRPSLESTASVS HSSSPTTLSM*FSRGRLIIVRNLNGSGATVKGPT PSCGATGFSSSTSLP*CGRQACLSSAAPKTSSI SRTPWHWGKQRRRH*STSE*SLTKPSVRAGKPK *TGWPTTCPKTTGSSGSSQPWAQEEAAAGRGDQA HWS*RG*RA*TAPNGKEPTWLPFVYTGYLFMT* NSLRS*TAINGNASFMQRRCWAPRGCTWLSAED CHPKSSSWWIWAQQRLFSSRGNLLPRPPARLPGS WRLAVTWCLLSDRMP*SSCDPPCVSSLD*VLAA PRGSRGTL*IQGCLLSTFQVGLGYENSLIFLYC KVILFAGKKIIDDSPHLYGWGDQAQPHKGESTLV LRTC*RDLGLM*LTPVTHEGKSRSEANTLPLSQ KSFFFF*DGVFLCCPGWSAVVQSWLTVTSASQV QVILLPQPPE*LGLQVCTTVPS*FLYFSRDGGFT MLAGLVSNS*PQVIHPPEPPKVLGLQA*ATAPG PLCHCLSHL*KQVLTKG*GQNSSRTITFGNLL* EC*GTRSVRTRGRAGIFLSDYT*FEMVQA*S*AS KGLDLSSFRNAGSRASPSLQKPTNSADGARTVG F*QPTGDPNISGHGLRTCPVMLLISQRSSKTQQ SQK*PPAQRLRCQLCSDSPGVRQ*SLPPAGHLPG HTPVPSKC*NSLDRLPVSKWGKPCVRVIYLSAP VCAGVGAHFAKPQRFWFWVYSLVCLARRIFSIF LSHFMFLSGEGKLVKYH*CGLRPALCETLK*EVK ADEKKEKAFLCSFMFSAQKETRECRFKTKTGEK TNPAPAGVS*NQNFIVADTLSDLS*VYTSAKGN RAATA*GHPLGVSVCPLSKQSSYRDGTWRMATHS TATSVLRTSSQGHRTVFKMIAHLF*KSKILHVT KSKVLCGF*TMTVCLLSCPTTKQSSRAPGGEGF QPP*WQGVLWGGERREQAVLENSSTLTSGHASLL DVLSTDRSGLLPSPKGVVSRSAGHLSVSPFTSR KMYI*A*TTRLHTVRPGHCPGSCCRRRSVEKGP GTPHPTPN*EQVESQRQGPATCSYPDPVS*AAQL EACGPRLPAILRPEQGLGRGWAGWASPSGVWGG RTRREEPVLRAPNFTLHSSPGLH*HKGHKESDL FTKY*KVS*SSTQYPPSCHYLYMH*FGKNKNFF* KKKKMITGWE*ACFTLCQRAQLAWLFWSARGQC PLPGGRLQTQTRGLRRWEAPVRAGWRPERTCQL LVGLKSATRQRVGGEPRVLHTKHSDDRGGEGSAH LLAEMTLYIVDPWDWENGWQQSLRKQKPRWPGA RVSS*GWSHPTPAAAAGLRSGGRPAPPPPPRCS PTRRW*CHPLLRRPRFPLPLPLPPPPHCCAGPHR

Sequence PIK3CD.fAug05

CTCGCGCCCAGCGCAGTCGCTCCGAGCGGCCGCGAGCAGAGCCGCCCAGCCCTGCCAGCTGCGCCGGGACGCTGGTGGCCATGACTGGCTTTCGTGGATG TGTATGTTCCTGAGGAAAGATGATTTGCTGTGCGTGCTCCTGTGGGGAGGACATGCAGTGTCTGCCTGGGAGAAGAGGCAGCTGAGGCCGTGGCCCGGAG TAGTAGGAGCCACAAGCCACCCTGGGCAGGACGAGGCCCTGAGGGAGGACAACTGTCATCTGGGAAGTAACAACGCAGGATGCCCCCTGGGGTGGACTGC CCCATGGAATTCTGGACCAAGGAGGAGAATCAGAGCGTTGTGGTTGACTTCCTGCTGCCCACAGGGGTCTACCTGAACTTCCCTGTGTCCCGCAATGCCA ACCTCAGCACCATCAAGCAGCTGCTGTGGCACCGCGCCCAGTATGAGCCGCTCTTCCACATGCTCAGTGGCCCCGAGGCCTATGTGTTCACCTGCATCAA CCAGACAGCGGAGCAGCAAGAGCTGGAGGACGAGCAACGGCGTCTGTGTGACGTGCAGCCCTTCCTGCCCGTCCTGCGCCTGGTGGCCCGTGAGGGCGAC CGCGTGAAGAAGCTCATCAACTCACAGATCAGCCTCCTGAGTAGCTGGGATTACAGGTGTGCACCACCGTACCCAGCTAATTTTTGTATTTTAGTAGAGA CGGGGGTTTCACCATGTTGGCTGGGCTGGTCTCGAACTCCTGACCTCAGGTGATCCACCCGCCTGAGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCC ACCGCGCCCGGCCCACTCTGCCATTGTCTAAGCCACCTCTGAAAGCAGGTTTTAACAAAAGGATGAGGCCAGAACTCTTCCAGAACCATCACCTTTGGGA ACCTGCTGTGAGAGTGCTGAGGTACCAGAAGTGTGAGAACGAGGGGGCGTGCTGGGATCTTTCTCTCTGACTATACTTAGTTTGAAATGGTGCAGGCTTA GTCTTAAGCCTCCAAAGGCCTGGATTTGAGCAGCTTTAGAAATGCAGGTTCTAGGGCTTCTCCCAGCCTTCAGAAGCCAACTAACTCTGCAGATGGGGCT AGGACTGTGGGCTTTTAGCAGCCCACAGGTGATCCTAACATATCAGGCCATGGACTCAGGACCTGCCCGGTGATGCTGTTGATTTCTCAAAGGTCTTCCA AAACTCAACAGAGCCAGAAGTAGCCGCCCGCTCAGCGGCTCAGGTGCCAGCTCTGTTCTGATTCACCAGGGGTCCGTCAGTAGTCATTGCCACCCGCGGG GCACCTCCCTGGCCACACGCCTGTTCCCAGCAAGTGCTGAAACTCACTAGACCGTCTGCCTGTTTCGAAATGGGGAAAGCCGTGCGTGCGCGTTATTTAT TTAAGTGCGCCTGTGTGCGCGGGTGTGGGAGCACACTTTGCAAAGCCACAGCGTTTCTGGTTTTGGGTGTACAGTCTTGTGTGCCTGGCGAGAAGAATAT TTTCTATTTTTTTAAGTCATTTCATGTTTCTGTCTGGGGAAGGCAAGTTAGTTAAGTATCACTGATGTGGGTTGAGACCAGCACTCTGTGAAACCTTGAA ATGAGAAGTAAAGGCAGATGAAAAGAAAGAAAAAGCCTTTTTATGTTCTTTTATGTTCTCGGCTCAAAAAGAAACAAGGGAGTGTAGGTTTAAAACCAAA ACAGGAGAGAAGACAAACCCCGCTCCGGCTGGAGTTAGTTAGAACCAGAACTTTATTGTAGCGGATACACTTTCTGACCTATCATGAGTATACACATCTG CGAAGGGAAACCGCGCGGCGACAGCGTGAGGACATCCCCTGGGCGTGAGCGTCTGTCCGCTGTCTAAACAGAGCAGCTACAGGGACGGGACATGGAGGAT GGCCACACATAGCACAGCCACCAGTGTCCTCAGAACTAGCAGTCAGGGTCACAGAACAGTATTCAAAATGATTGCCCACCTGTTTTAGAAATCTAAAATT TTACATGTAACTAAGAGCAAAGTGCTATGTGGGTTTTAGACCATGACTGTTTGTTTGCTCTCCTGCCCTACCACCAAGCAAAGCAGCAGGGCTCCTGGGG GAGAGGGATTTCAACCCCCCTGATGGCAGGGGGTGCTCTGGGGAGGAGAGAGGAGAGAACAGGCTGTTTTGGAAAATTCCAGCACTTTGACTTCGGGCCA TGCGTCTCTCCTGGACGTTCTGAGTACGGATCGCTCAGGCCTCCTGCCGTCTCCAAAAGGAGTGGTCAGCCGGTCTGCAGGACACCTCTCAGTTTCTCCT TTTACCAGCAGGAAAATGTACATTTAAGCATAAACCACAAGGCTGCACACGGTGAGACCAGGCCACTGCCCAGGGAGCTGCTGTCGGAGGAGGAGTGTGG AAAAGGGGCCAGGGACCCCGCACCCCACACCAAATTAGGAACAGGTAGAATCCCAAAGGCAAGGCCCGGCCACTTGTAGCTACCCTGATCCAGTCTCTTG AGCAGCACAGCTAGAGGCCTGTGGCCCCCGACTCCCAGCCATACTCAGACCTGAGCAGGGGCTGGGGCGGGGCTGGGCGGGGTGGGCCTCTCCCTCTGGG GTCTGGGGAGGGAGGACCCGGCGGGAAGAGCCCGTGCTCAGAGCTCCGAACTTCACTTTACATTCCAGTCCCGGGTTACACTAGCACAAAGGACACAAGG AATCCGATTTATTTACAAAATATTAAAAAGTCAGTTAATCATCTACACAGTACCCCCCATCCTGCCATTATTTATACATGCACTAGTTTGGAAAAAATAA AAACTTTTTTTAAAAAAAGAAAAAAATGATTACCGGGTGGGAGTGAGCATGTTTTACCCTTTGTCAACGAGCCCAGCTGGCATGGCTTTTCTGGAGTGCG AGGGGCCAGTGTCCTCTCCCCGGGGGGAGACTCCAGACACAAACGCGGGGCCTGAGGCGCTGGGAGGCCCCTGTGCGAGCCGGATGGCGGCCAGAGAGGA CGTGTCAGCTCCTCGTGGGACTGAAGAGCGCGACCAGGCAGAGGGTGGGCGGGGAGCCTAGGGTCCTACACACCAAGCACAGCGACGATCGTGGCGGGGA GGGGTCTGCACACCTACTGGCCGAAATGACTTTGTACATCGTGGACCCTTGGGACTGGGAGAACGGATGGCAGCAGAGTCTTCGAAAGCAGAAACCGAGG TGGCCGGGGGCACGGGTCAGTAGCTGAGGGTGGAGTCATCCCACTCCAGCTGCTGCTGCCGGGTTGCGTTCTGGGGGTCGCCCTGCTCCCCCTCCTCCTC CTCGCTGCTCTCCGACTCGGCGCTGGTGATGTCATCCTCTTCTTCGCCGTCCTCGCTTTCCTCTTCCTCTTCCTCTTCCTCCTCCTCCTCACTGCTGTGC TGGTCCTCATAGG

PIK3CD.F1 (forward primer)
PIK3CD.R1 (reverse primer)
Mutually exclusive exon skipping

Frame 0

LAPSAVAPSGREQSRPALPAAPGRWWP*LAFVD VYVPEER*FAVRAPVGRTCSVCLGEEAAEAVARS SRSHKPPWAGRGPEGGQLSSGK*QRRMPPGVDC PMEFWTKEENQSVVVDFLLPTGVYLNFPVSRNA NLSTIKQLLWHRAQYEPLFHMLSGPEAYVFTCIN QTAEQQELEDEQRRLCDVQPFLPVLRLVAREGD RVKKLINSQISLLSSWDYRCAPPYPANFCILVE TGVSPCWLGWSRTPDLR*STRLSLPKCWDYRHEP PRPAHSAIV*ATSESRF*QKDEARTLPEPSPLG TCCESAEVPEV*ERGGVLGSFSLTILSLKWCRL SLKPPKAWI*AALEMQVLGLLPAFRSQLTLQMGL GLWAFSSPQVILTYQAMDSGPAR*CC*FLKGLP KLNRARSSRPLSGSGASSVLIHQGSVSSHCHPR GTSLATRLFPASAETH*TVCLFRNGESRACALFI *VRLCARVWEHTLQSHSVSGFGCTVLCAWREEY FLFF*VISCFCLGKAS*LSITDVG*DQHSVKP* NEK*RQMKRKKKPFYVLLCSRLKKKQGSVGLKPK QERRQTPLRLELVRTRTLL*RIHFLTYHEYTHL RRETARRQREDIPWA*ASVRCLNRAATGTGHGG WPHIAQPPVSSELAVRVTEQYSK*LPTCFRNLKF YM*LRAKCYVGFRP*LFVCSPALPPSKAAGLLG ERDFNPPDGRGCSGEERGENRLFWKIPAL*LRA MRLSWTF*VRIAQASCRLQKEWSAGLQDTSQFLL LPAGKCTFKHKPQGCTR*DQATAQGAAVGGGVW KRGQGPRTPHQIRNR*NPKGKARPLVATLIQSL EQHS*RPVAPDSQPYSDLSRGWGGAGRGGPLPLG SGEGGPGGKSPCSELRTSLYIPVPGYTSTKDTR NPIYLQNIKKSVNHLHSTPHPAIIYTCTSLEKI KTFFKKRKK*LPGGSEHVLPFVNEPSWHGFSGVR GASVLSPGGDSRHKRGA*GAGRPLCEPDGGQRG RVSSSWD*RARPGRGWAGSLGSYTPSTATIVAG RGLHTYWPK*LCTSWTLGTGRTDGSRVFESRNRG GRGHGSVAEGGVIPLQLLLPGCVLGVALLPLLL LAALRLGAGDVILFFAVLAFLFLFLFLLLLTAV LVLI

Frame 1

SRPAQSLRAAASRAAQPCQLRRDAGGHDWLSWM CMFLRKDDLLCVLLWGGHAVSAWEKRQLRPWPG VVGATSHPGQDEALREDNCHLGSNNAGCPLGWTA PWNSGPRRRIRALWLTSCCPQGST*TSLCPAMP TSAPSSSCCGTAPSMSRSSTCSVAPRPMCSPAS TRQRSSKSWRTSNGVCVTCSPSCPSCAWWPVRAT A*RSSSTHRSAS*VAGITGVHHRTQLIFVF**R RGFHHVGWAGLELLTSGDPPA*ASQSAGITGMS HRARPTLPLSKPPLKAGFNKRMRPELFQNHHLWE PAVRVLRYQKCENEGACWDLSL*LYLV*NGAGL VLSLQRPGFEQL*KCRF*GFSQPSEAN*LCRWG *DCGLLAAHR*S*HIRPWTQDLPGDAVDFSKVFQ NSTEPEVAARSAAQVPALF*FTRGPSVVIATRG APPWPHACSQQVLKLTRPSACFEMGKAVRARYL FKCACVRGCGSTLCKATAFLVLGVQSCVPGEKNI FYFFKSFHVSVWGRQVS*VSLMWVETSTL*NLE MRSKGR*KERKSLFMFFYVLGSKRNKGV*V*NQ NRREDKPRSGWS*LEPELYCSGYTF*PIMSIHIC EGKPRGDSVRTSPGRERLSAV*TEQLQGRDMED GHT*HSHQCPQN*QSGSQNSIQNDCPPVLEI*N FTCN*EQSAMWVLDHDCLFALLPYHQAKQQGSWG RGISTPLMAGGALGRREERTGCFGKFQHFDFGP CVSPGRSEYGSLRPPAVSKRSGQPVCRTPLSFS FYQQENVHLSINHKAAHGETRPLPRELLSEEECG KGARDPAPHTKLGTGRIPKARPGHL*LP*SSLL SSTARGLWPPTPSHTQT*AGAGAGLGGVGLSLW GLGREDPAGRARAQSSELHFTFQSRVTLAQRTQG IRFIYKILKSQLIIYTVPPILPLFIHALVWKK* KLFLKKEKNDYRVGVSMFYPLSTSPAGMAFLEC EGPVSSPRGETPDTNAGPEALGGPCASRMAARED VSAPRGTEERDQAEGGRGA*GPTHQAQRRSWRG GVCTPTGRNDFVHRGPLGLGERMAAESSKAETE VAGGTGQ*LRVESSHSSCCCRVAFWGSPCSPSSS SLLSDSALVMSSSSSPSSLSSSSSSSSSSSLLC WSS*

Frame 2

RAQRSRSERPRAEPPSPASCAGTLVAMTGFRGC VCS*GKMICCACSCGEDMQCLPGRRGS*GRGPE **EPQATLGRTRP*GRTTVIWEVTTQDAPWGGL PHGILDQGGESERCG*LPAAHRGLPELPCVPQCQ PQHHQAAAVAPRPV*AALPHAQWPRGLCVHLHQ PDSGAARAGGRATASV*RAALPARPAPGGP*GR PREEAHQLTDQPPE*LGLQVCTTVPS*FLYFSRD GGFTMLAGLVSNS*PQVIHPPEPPKVLGLQA*A TAPGPLCHCLSHL*KQVLTKG*GQNSSRTITFG NLL*EC*GTRSVRTRGRAGIFLSDYT*FEMVQA* S*ASKGLDLSSFRNAGSRASPSLQKPTNSADGA RTVGF*QPTGDPNISGHGLRTCPVMLLISQRSS KTQQSQK*PPAQRLRCQLCSDSPGVRQ*SLPPAG HLPGHTPVPSKC*NSLDRLPVSKWGKPCVRVIY LSAPVCAGVGAHFAKPQRFWFWVYSLVCLARRI FSIFLSHFMFLSGEGKLVKYH*CGLRPALCETLK *EVKADEKKEKAFLCSFMFSAQKETRECRFKTK TGEKTNPAPAGVS*NQNFIVADTLSDLS*VYTS AKGNRAATA*GHPLGVSVCPLSKQSSYRDGTWRM ATHSTATSVLRTSSQGHRTVFKMIAHLF*KSKI LHVTKSKVLCGF*TMTVCLLSCPTTKQSSRAPG GEGFQPP*WQGVLWGGERREQAVLENSSTLTSGH ASLLDVLSTDRSGLLPSPKGVVSRSAGHLSVSP FTSRKMYI*A*TTRLHTVRPGHCPGSCCRRRSV EKGPGTPHPTPN*EQVESQRQGPATCSYPDPVS* AAQLEACGPRLPAILRPEQGLGRGWAGWASPSG VWGGRTRREEPVLRAPNFTLHSSPGLH*HKGHK ESDLFTKY*KVS*SSTQYPPSCHYLYMH*FGKNK NFF*KKKKMITGWE*ACFTLCQRAQLAWLFWSA RGQCPLPGGRLQTQTRGLRRWEAPVRAGWRPER TCQLLVGLKSATRQRVGGEPRVLHTKHSDDRGGE GSAHLLAEMTLYIVDPWDWENGWQQSLRKQKPR WPGARVSS*GWSHPTPAAAAGLRSGGRPAPPPP PRCSPTRRW*CHPLLRRPRFPLPLPLPPPPHCCA GPHR

Sequence PIK3CD.bAug05

Frame 0

Frame 1

Frame 2

Sequence PIK3CD.fAug05

Frame 0

Frame 1

Frame 2

Sequence PIK3CD.eAug05

Frame 0

Frame 1

Frame 2