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Sequences

Sequence BID.hAug05

GAAGGAGGAAGCGCGCGGGGCGCCATAAGGAGGAAGCGGGTAGTCGACCGTGTCCGCGCGCCTGGGAGACGCTGCCTCGGCCCGGACGCGCCCGCGCCCC CGCGGCTGGAGGGTGATTCTGAAAGTCAAGAAGACATCATCCGGAATATTGCCAGGCACCTCGCCCAGGTCGGGGACAGCATGGACCGTAGCATCCCTCC GGGCCTGGTGAACGGCCTGGCCCTGCAGCTCAGGAACACCAGCCGGTCGGAGGAGGACCGGAACAGGGACCTGGCCACTGCCCTGGAGCAGCTGCTGCAG GCCTACCCTAGAGACATGGAGAAGGAGAAGACCATGCTGGTGCTGGCCCTGCTGCTGGCCAAGAAGGTGGCCAGTCACACGCCGTCCTTGCTCCGTGATG TCTTTCACACAACAGTGAATTTTATTAACCAGAACCTACGCACCTACGTGAGGAGCTTAGCCAGAAATGGGATGGACTGAACGGACAGTTCCAGAAGTGT GACTGGCTAAAGCTCGATGTGGTCACAGCTGTATAGCTGCTTCCAGTGTAGACGGAGCCCTGGCATGTCAACAGCGTTCCTAGAGAAGACAGGCTGGAAG ATAGCTGTGACTTCTATTTTAAAGACAATGTTAAACTTATAACCCACTTTAAAATATCTACATTAATATACTTGAATGAAAATGTCCATTTACACGTATT TGAATGGCCTTCATATCATCCACACATGAATCTGCACATCTGTAAATCTACACACGGTGCCTTTATTTCCACTGTGCAGGTTCCCACTTAAAAATTAAAT TGGAAAGCAGGTTTCAAGGAAGTAGAAACAAAATACAATTTTTTTGGTAAAAAAAAATTACTGTTTATTAAAGTACAACCATAGAGGATGGTCTTACAGC AGGCAGTATCCTGTTTGAGGAAAGCAAGAATCAGAGAAGGAACATACCCCTTACAAATGAAAAATTCCACTCAAAATAGGGACTATCTATCTTAATACTA AGGAACCAACAATCTTCCTGTTTAAAAAACCACATGGCACAGAGATTCTGAACTAAAGTGCTGCACTCAAATGATGGGAAGTCCGGCCCCAGTACACAGG GGCTTGACTTTTTCAACTTCGTTTCCTTTGTTGGAGTCAAAAAGAACCACTTGTGGTTCTAAAAGGTGTGAAGGTGATTTAAGGGCCCAGGTCAGCCACT GTTTGTTTACAAAATCAGGTAACTAACTGCATACACTTTTTCTCTTTCCATGACATCAAGACTTTGCTAAAGACATGAAGCCACGGGTGCCAGAAGCTAC TGCGATGCCCCGGGAGTTAGCCCCCTGGTAATAGCTGTAAACTTCCAATTTCTAGCCATACGCTCAGCTCATCCATGCCTCAGAAGTGCATCTGGAGAGA ACAGGTTTCTAAGCATAAAAGATGAAAGAGCAGTTGGACTTTTTAAAAATTCAGCAAAGTGGTTCCCTCTCTTAGGGACAGTCAAAACCAAGTCACTTAG GTAGTACCAAAATAAATAAGGAAAAGCTTAGCTTTAGAAACAGTGCAACACTGGTCTGCTGTTCCAGTGGTAAGCTATGTCCCAGGAATCAGTTTAAAAG CACGACAGTGGATGCTGGGTCCATATCACACACATTGCTGTGAACAGGAAACTCCTGTGACCACAACATGAGGCCACTGGAGACGCATATGAGTAAGGGC ACTGACGGACTCATGATTTCTTCTTACCAGATGCTTTCCTGTTCTTTAAGAGTTTAAAATCATCAGAAAGGAAAAACAAACTCTATATTGTTCAGCATGC AATACATACCACGCTAGGGCTGGCTCAATTGAAAGTGGGCAAAAGCTTACAAATACTAAAAAGAAGTGCTGCCGCGCAGTGTGGAGGCCACTGTTTGGAA ATAAATCTTCCTAACACTACGACTTTTCATGTTTTGGAGTGGACTTTTAAAAAAGTTTTCCAACCACAATGGAAACAGGTTGAACCACACTGCGCCTACA CATCCATCCATACGTCAGCCATTATAATCAGATTCTCACTGGGGGAGGCATTTTCTTCCCAAAGGAGCTGTATGCAGTCATCACCCGGAACTAATGGGAA AGTGTTCTTAAGCAGCAAGTGACTCTGGACCACCTCTCTCACACACTGACTCTTCCACAGCATCTTCACCTGCTTCTGTTTCACTTGAATTCATACAGTA GGCCACCATTTTATAACAGGTCTTAGTGTAAATTCTTCCAGACCAAGTCTGAGATAGTCTTGGTTGTAAAGCAAGACCGTCACCCCTTCTCCCAAAGCCA ACATTATAAAAAGATTCCATTGTCTCAAACACAAATGTTCCAGTCTTGAGCACTTCCCAGCCATGGTGACTCGGGCATATCCTGGCAGTTGGTGGATTTT GCCCTGTAACACAATCCTTGTGGATATGATTCAAAGGTGACTTTAATTCAGAGAATGTGTTTTCCTTTCTGTTTTAAAAATATTTGAATTAAGTGATGGA AATTCTTTTGTTAAACATCAAAAGCTTTCTCCTTGAAAAGATGCTTGGTGTATCTGAAAGTCACGTGGGACATTTGGTGCATTCAGAAGGTCAAATCATT CCTGACCTCTCCTCTTAGGACCCAGCATTAACTGCTGCGATACTCAAGGCTTCCTTAAAGGATCACAGCACTGGCGCCTAGTTTTACTGTCCAGCAACTT TATTAGTTGTGCTAGCTGAGAATTGGAATATAGAGTCCTTTGAATGTTACGATCTTGTTTTCAAAACCTGGAGTCAAACCAATTAAGTATGGACTTAACA AAGACAATTGATGATGTGAAATGATACCACCTGTCAAGGGCATAGTTACCATGTGGATAGAAAGATGAGTCCACTGATTATCTTCAAATCCCATGTATTC TCTCTAGCACGTTGCTTCTGACAGAAAGAATAACTCACAGATTTGAAGTTTGTTCCTTTCCCCTGATGAAGAGTAGATTGGCCAAAAGGTAGATGACAGG AAGGAAGAGGAGCCAGAATGACCCACAGTGATCAGTTCAGCAGTGCAGAGCGGCAGTGCAGAGGCTCATGGAACTCACACGGAAAAAGGAAACACATGCT GCTTAAAATGCACGATAACTTAATTATCCAAATTACCATTCAATTCCGGTATTTTTGAGATGAAAGCATTTGAGCCAACACTAAATTGACCTTAATTAAG GAATGAGGTAGGAAGAGGCACTTTTAATCTGGAGGAAGAAATAAATGGAAGACTGTAATAGGAAAAATACAGGTCTAGGAAAAAAATAATTTTTTTCCTT TGATGCTGGCCAAGGACCCCTCTACATAATACAGTTCTCCTGCTGGAAAGCATCAGGGCAAACAGAGTTATTCCAAACTCTGCAACAGGGAAGACTGTAC TTTGACCTTCTTTTCCTGGGGAAAGGGAACAACTAGTAATCTTTAAATGTCAAAACTTTTCTTCTAAATAGTACATTCATTAACCAGTTTCTTCTGGTGG TGCAAAGAACACTCACCACAGTGAGCAATACCACTATTCAATTTCCCCTTTGGAGAGGTGACAGCGGGGTAGAATCACCTGGAGTCAGCTGCTAGTCTTT AACTGATGACAAGGCCTTGCAAATACCCCTGAGAACAATTTCACTCTGAGCTCACAGGGAAGCAGCAGTCTGGTGTGGGCTCCTGTGGTGTAACCATCAA ATGTGGAAGACCTGATGGTGTTAGTGTGTGGGGATGCCGGGCTGAAATTCAACAGCCTTGCAAAAAGAAAGGGAAGATGGGATGTGTTAATATTGTAGTG GCATGCACTTTCCAGCTCTCCATGGAGCTCTCTTTATATATGAGGTAATTGAATGTCACTATTCTGTACCATACAGCAGGTATTATCTAGCCAAGGTTAT AATTACCATGAAGTGCTGTGGAATGTAGTAGGAGGCTTTTCACTGGG

BID.F1 (forward primer)
BID.R1 (reverse primer)
Single exon skipping

Frame 0

EGGSARGAIRRKRVVDRVRAPGRRCLGPDAPAP PRLEGDSESQEDIIRNIARHLAQVGDSMDRSIPP GLVNGLALQLRNTSRSEEDRNRDLATALEQLLQ AYPRDMEKEKTMLVLALLLAKKVASHTPSLLRD VFHTTVNFINQNLRTYVRSLARNGMD*TDSSRSV TG*SSMWSQLYSCFQCRRSPGMSTAFLEKTGWK IAVTSILKTMLNL*PTLKYLH*YT*MKMSIYTY LNGLHIIHT*ICTSVNLHTVPLFPLCRFPLKN*I GKQVSRK*KQNTIFLVKKNYCLLKYNHRGWSYS RQYPV*GKQESEKEHTPYK*KIPLKIGTIYLNT KEPTIFLFKKPHGTEILN*SAALK*WEVRPQYTG A*LFQLRFLCWSQKEPLVVLKGVKVI*GPRSAT VCLQNQVTNCIHFFSFHDIKTLLKT*SHGCQKL LRCPGS*PPGNSCKLPISSHTLSSSMPQKCIWRE QVSKHKR*KSSWTF*KFSKVVPSLRDSQNQVT* VVPK*IRKSLALETVQHWSAVPVVSYVPGISLK ARQWMLGPYHTHCCEQETPVTTT*GHWRRI*VRA LTDS*FLLTRCFPVL*EFKIIRKEKQTLYCSAC NTYHARAGSIESGQKLTNTKKKCCRAVWRPLFG NKSS*HYDFSCFGVDF*KSFPTTMETG*TTLRLH IHPYVSHYNQILTGGGIFFPKELYAVITRN*WE SVLKQQVTLDHLSHTLTLPQHLHLLLFHLNSYS RPPFYNRS*CKFFQTKSEIVLVVKQDRHPFSQSQ HYKKIPLSQTQMFQS*ALPSHGDSGISWQLVDF AL*HNPCGYDSKVTLIQRMCFPFCFKNI*IK*W KFFC*TSKAFSLKRCLVYLKVTWDIWCIQKVKSF LTSPLRTQH*LLRYSRLP*RITALAPSFTVQQL Y*LC*LRIGI*SPLNVTILFSKPGVKPIKYGLN KDN**CEMIPPVKGIVTMWIER*VH*LSSNPMYS L*HVASDRKNNSQI*SLFLSPDEE*IGQKVDDR KEEEPE*PTVISSAVQSGSAEAHGTHTEKGNTC CLKCTIT*LSKLPFNSGIFEMKAFEPTLN*P*LR NEVGRGTFNLEEEINGRL**EKYRSRKKIIFFL *CWPRTPLHNTVLLLESIRANRVIPNSATGKTV L*PSFPGEREQLVIFKCQNFSSK*YIH*PVSSGG AKNTHHSEQYHYSISPLER*QRGRITWSQLLVF N**QGLANTPENNFTLSSQGSSSLVWAPVV*PS NVEDLMVLVCGDAGLKFNSLAKRKGRWDVLIL*W HALSSSPWSSLYI*GN*MSLFCTIQQVLSSQGY NYHEVLWNVVGGFSL

Frame 1

KEEARGAP*GGSG*STVSARLGDAASARTRPRP RGWRVILKVKKTSSGILPGTSPRSGTAWTVASL RAW*TAWPCSSGTPAGRRRTGTGTWPLPWSSCCR PTLETWRRRRPCWCWPCCWPRRWPVTRRPCSVM SFTQQ*ILLTRTYAPT*GA*PEMGWTERTVPEV *LAKARCGHSCIAASSVDGALACQQRS*RRQAGR *L*LLF*RQC*TYNPL*NIYINILE*KCPFTRI *MAFISSTHESAHL*IYTRCLYFHCAGSHLKIK LESRFQGSRNKIQFFW*KKITVY*STTIEDGLTA GSILFEESKNQRRNIPLTNEKFHSK*GLSILIL RNQQSSCLKNHMAQRF*TKVLHSNDGKSGPSTQ GLDFFNFVSFVGVKKNHLWF*KV*R*FKGPGQPL FVYKIR*LTAYTFSLSMTSRLC*RHEATGARSY CDAPGVSPLVIAVNFQFLAIRSAHPCLRSASGE NRFLSIKDERAVGLFKNSAKWFPLLGTVKTKSLR *YQNK*GKA*L*KQCNTGLLFQW*AMSQESV*K HDSGCWVHITHIAVNRKLL*PQHEATGDAYE*G H*RTHDFFLPDAFLFFKSLKSSERKNKLYIVQHA IHTTLGLAQLKVGKSLQILKRSAAAQCGGHCLE INLPNTTTFHVLEWTFKKVFQPQWKQVEPHCAY TSIHTSAIIIRFSLGEAFSSQRSCMQSSPGTNGK VFLSSK*LWTTSLTH*LFHSIFTCFCFT*IHTV GHHFITGLSVNSSRPSLR*SWL*SKTVTPSPKA NIIKRFHCLKHKCSSLEHFPAMVTRAYPGSWWIL PCNTILVDMIQR*L*FRECVFLSVLKIFELSDG NSFVKHQKLSP*KDAWCI*KSRGTFGAFRRSNH S*PLLLGPSINCCDTQGFLKGSQHWRLVLLSSNF ISCAS*ELEYRVL*MLRSCFQNLESNQLSMDLT KTIDDVK*YHLSRA*LPCG*KDESTDYLQIPCI LSSTLLLTERITHRFEVCSFPLMKSRLAKR*MTG RKRSQNDPQ*SVQQCRAAVQRLMELTRKKETHA A*NAR*LNYPNYHSIPVFLR*KHLSQH*IDLN* GMR*EEALLIWRKK*MEDCNRKNTGLGKK*FFSF DAGQGPLYIIQFSCWKASGQTELFQTLQQGRLY FDLLFLGKGNN**SLNVKTFLLNSTFINQFLLV VQRTLTTVSNTTIQFPLWRGDSGVESPGVSC*SL TDDKALQIPLRTISL*AHREAAVWCGLLWCNHQ MWKT*WC*CVGMPG*NSTALQKEREDGMC*YCS GMHFPALHGALFIYEVIECHYSVPYSRYYLAKVI ITMKCCGM**EAFHW

Frame 2

RRKRAGRHKEEAGSRPCPRAWETLPRPGRARAP AAGG*F*KSRRHHPEYCQAPRPGRGQHGP*HPS GPGERPGPAAQEHQPVGGGPEQGPGHCPGAAAA GLP*RHGEGEDHAGAGPAAGQEGGQSHAVLAP*C LSHNSEFY*PEPTHLREELSQKWDGLNGQFQKC DWLKLDVVTAV*LLPV*TEPWHVNSVPREDRLE DSCDFYFKDNVKLITHFKISTLIYLNENVHLHVF EWPSYHPHMNLHICKSTHGAFISTVQVPT*KLN WKAGFKEVETKYNFFGKKKLLFIKVQP*RMVLQ QAVSCLRKARIREGTYPLQMKNSTQNRDYLS*Y* GTNNLPV*KTTWHRDSELKCCTQMMGSPAPVHR GLTFSTSFPLLESKRTTCGSKRCEGDLRAQVSH CLFTKSGN*LHTLFLFP*HQDFAKDMKPRVPEAT AMPRELAPW**L*TSNF*PYAQLIHASEVHLER TGF*A*KMKEQLDFLKIQQSGSLS*GQSKPSHL GSTKINKEKLSFRNSATLVCCSSGKLCPRNQFKS TTVDAGSISHTLL*TGNSCDHNMRPLETHMSKG TDGLMISSYQMLSCSLRV*NHQKGKTNSILFSM QYIPR*GWLN*KWAKAYKY*KEVLPRSVEATVWK *IFLTLRLFMFWSGLLKKFSNHNGNRLNHTAPT HPSIRQPL*SDSHWGRHFLPKGAVCSHHPELMG KCS*AASDSGPPLSHTDSSTASSPASVSLEFIQ* ATIL*QVLV*ILPDQV*DSLGCKARPSPLLPKP TL*KDSIVSNTNVPVLSTSQPW*LGHILAVGGF CPVTQSLWI*FKGDFNSENVFSFLF*KYLN*VME ILLLNIKSFLLEKMLGVSESHVGHLVHSEGQII PDLSS*DPALTAAILKASLKDHSTGA*FYCPAT LLVVLAENWNIESFECYDLVFKTWSQTN*VWT*Q RQLMM*NDTTCQGHSYHVDRKMSPLIIFKSHVF SLARCF*QKE*LTDLKFVPFP**RVDWPKGR*Q EGRGARMTHSDQFSSAERQCRGSWNSHGKRKHML LKMHDNLIIQITIQFRYF*DESI*ANTKLTLIK E*GRKRHF*SGGRNKWKTVIGKIQV*EKNNFFP LMLAKDPST*YSSPAGKHQGKQSYSKLCNREDCT LTFFSWGKGTTSNL*MSKLFF*IVHSLTSFFWW CKEHSPQ*AIPLFNFPFGEVTAG*NHLESAASL *LMTRPCKYP*EQFHSELTGKQQSGVGSCGVTIK CGRPDGVSVWGCRAEIQQPCKKKGKMGCVNIVV ACTFQLSMELSLYMR*LNVTILYHTAGII*PRL *LP*SAVECSRRLFTG

Sequence BID.eAug05

GAAGGAGGAAGCGCGCGGGGCGCCATAAGGAGGAAGCGGGTAGTCGACCGTGTCCGCGCGCCTGGGAGACGCTGCCTCGGCCCGGACGCGCCCGCGCCCC CGCGGCTGGAGGGTGGTCAACAACGGTTCCAGCCTCAGGGATGAGTGCATCACAAACCTACTGGTGTTTGGCTTCCTCCAAAGCTGTTCTGACAACAGCT TCCGCAGAGAGCTGGACGCACTGGGCCACGAGCTGCCAGTGCTGGCTCCCCAGTGGGAGGGCTACGATGAGCTGCAGACTGATGGCAACCGCAGCAGCCA CTCCCGCTTGGGAAGAATAGAGGCAGATTCTGAAAGTCAAGAAGACATCATCCGGAATATTGCCAGGCACCTCGCCCAGGTCGGGGACAGCATGGACCGT AGCATCCCTCCGGGCCTGGTGAACGGCCTGGCCCTGCAGCTCAGGAACACCAGCCGGTCGGAGGAGGACCGGAACAGGGACCTGGCCACTGCCCTGGAGC AGCTGCTGCAGGCCTACCCTAGAGACATGGAGAAGGAGAAGACCATGCTGGTGCTGGCCCTGCTGCTGGCCAAGAAGGTGGCCAGTCACACGCCGTCCTT GCTCCGTGATGTCTTTCACACAACAGTGAATTTTATTAACCAGAACCTACGCACCTACGTGAGGAGCTTAGCCAGAAATGGGATGGACTGAACGGACAGT TCCAGAAGTGTGACTGGCTAAAGCTCGATGTGGTCACAGCTGTATAGCTGCTTCCAGTGTAGACGGAGCCCTGGCATGTCAACAGCGTTCCTAGAGAAGA CAGGCTGGAAGATAGCTGTGACTTCTATTTTAAAGACAATGTTAAACTTATAACCCACTTTAAAATATCTACATTAATATACTTGAATGAAAATGTCCAT TTACACGTATTTGAATGGCCTTCATATCATCCACACATGAATCTGCACATCTGTAAATCTACACACGGTGCCTTTATTTCCACTGTGCAGGTTCCCACTT AAAAATTAAATTGGAAAGCAGGTTTCAAGGAAGTAGAAACAAAATACAATTTTTTTGGTAAAAAAAAATTACTGTTTATTAAAGTACAACCATAGAGGAT GGTCTTACAGCAGGCAGTATCCTGTTTGAGGAAAGCAAGAATCAGAGAAGGAACATACCCCTTACAAATGAAAAATTCCACTCAAAATAGGGACTATCTA TCTTAATACTAAGGAACCAACAATCTTCCTGTTTAAAAAACCACATGGCACAGAGATTCTGAACTAAAGTGCTGCACTCAAATGATGGGAAGTCCGGCCC CAGTACACAGGGGCTTGACTTTTTCAACTTCGTTTCCTTTGTTGGAGTCAAAAAGAACCACTTGTGGTTCTAAAAGGTGTGAAGGTGATTTAAGGGCCCA GGTCAGCCACTGTTTGTTTACAAAATCAGGTAACTAACTGCATACACTTTTTCTCTTTCCATGACATCAAGACTTTGCTAAAGACATGAAGCCACGGGTG CCAGAAGCTACTGCGATGCCCCGGGAGTTAGCCCCCTGGTAATAGCTGTAAACTTCCAATTTCTAGCCATACGCTCAGCTCATCCATGCCTCAGAAGTGC ATCTGGAGAGAACAGGTTTCTAAGCATAAAAGATGAAAGAGCAGTTGGACTTTTTAAAAATTCAGCAAAGTGGTTCCCTCTCTTAGGGACAGTCAAAACC AAGTCACTTAGGTAGTACCAAAATAAATAAGGAAAAGCTTAGCTTTAGAAACAGTGCAACACTGGTCTGCTGTTCCAGTGGTAAGCTATGTCCCAGGAAT CAGTTTAAAAGCACGACAGTGGATGCTGGGTCCATATCACACACATTGCTGTGAACAGGAAACTCCTGTGACCACAACATGAGGCCACTGGAGACGCATA TGAGTAAGGGCACTGACGGACTCATGATTTCTTCTTACCAGATGCTTTCCTGTTCTTTAAGAGTTTAAAATCATCAGAAAGGAAAAACAAACTCTATATT GTTCAGCATGCAATACATACCACGCTAGGGCTGGCTCAATTGAAAGTGGGCAAAAGCTTACAAATACTAAAAAGAAGTGCTGCCGCGCAGTGTGGAGGCC ACTGTTTGGAAATAAATCTTCCTAACACTACGACTTTTCATGTTTTGGAGTGGACTTTTAAAAAAGTTTTCCAACCACAATGGAAACAGGTTGAACCACA CTGCGCCTACACATCCATCCATACGTCAGCCATTATAATCAGATTCTCACTGGGGGAGGCATTTTCTTCCCAAAGGAGCTGTATGCAGTCATCACCCGGA ACTAATGGGAAAGTGTTCTTAAGCAGCAAGTGACTCTGGACCACCTCTCTCACACACTGACTCTTCCACAGCATCTTCACCTGCTTCTGTTTCACTTGAA TTCATACAGTAGGCCACCATTTTATAACAGGTCTTAGTGTAAATTCTTCCAGACCAAGTCTGAGATAGTCTTGGTTGTAAAGCAAGACCGTCACCCCTTC TCCCAAAGCCAACATTATAAAAAGATTCCATTGTCTCAAACACAAATGTTCCAGTCTTGAGCACTTCCCAGCCATGGTGACTCGGGCATATCCTGGCAGT TGGTGGATTTTGCCCTGTAACACAATCCTTGTGGATATGATTCAAAGGTGACTTTAATTCAGAGAATGTGTTTTCCTTTCTGTTTTAAAAATATTTGAAT TAAGTGATGGAAATTCTTTTGTTAAACATCAAAAGCTTTCTCCTTGAAAAGATGCTTGGTGTATCTGAAAGTCACGTGGGACATTTGGTGCATTCAGAAG GTCAAATCATTCCTGACCTCTCCTCTTAGGACCCAGCATTAACTGCTGCGATACTCAAGGCTTCCTTAAAGGATCACAGCACTGGCGCCTAGTTTTACTG TCCAGCAACTTTATTAGTTGTGCTAGCTGAGAATTGGAATATAGAGTCCTTTGAATGTTACGATCTTGTTTTCAAAACCTGGAGTCAAACCAATTAAGTA TGGACTTAACAAAGACAATTGATGATGTGAAATGATACCACCTGTCAAGGGCATAGTTACCATGTGGATAGAAAGATGAGTCCACTGATTATCTTCAAAT CCCATGTATTCTCTCTAGCACGTTGCTTCTGACAGAAAGAATAACTCACAGATTTGAAGTTTGTTCCTTTCCCCTGATGAAGAGTAGATTGGCCAAAAGG TAGATGACAGGAAGGAAGAGGAGCCAGAATGACCCACAGTGATCAGTTCAGCAGTGCAGAGCGGCAGTGCAGAGGCTCATGGAACTCACACGGAAAAAGG AAACACATGCTGCTTAAAATGCACGATAACTTAATTATCCAAATTACCATTCAATTCCGGTATTTTTGAGATGAAAGCATTTGAGCCAACACTAAATTGA CCTTAATTAAGGAATGAGGTAGGAAGAGGCACTTTTAATCTGGAGGAAGAAATAAATGGAAGACTGTAATAGGAAAAATACAGGTCTAGGAAAAAAATAA TTTTTTTCCTTTGATGCTGGCCAAGGACCCCTCTACATAATACAGTTCTCCTGCTGGAAAGCATCAGGGCAAACAGAGTTATTCCAAACTCTGCAACAGG GAAGACTGTACTTTGACCTTCTTTTCCTGGGGAAAGGGAACAACTAGTAATCTTTAAATGTCAAAACTTTTCTTCTAAATAGTACATTCATTAACCAGTT TCTTCTGGTGGTGCAAAGAACACTCACCACAGTGAGCAATACCACTATTCAATTTCCCCTTTGGAGAGGTGACAGCGGGGTAGAATCACCTGGAGTCAGC TGCTAGTCTTTAACTGATGACAAGGCCTTGCAAATACCCCTGAGAACAATTTCACTCTGAGCTCACAGGGAAGCAGCAGTCTGGTGTGGGCTCCTGTGGT GTAACCATCAAATGTGGAAGACCTGATGGTGTTAGTGTGTGGGGATGCCGGGCTGAAATTCAACAGCCTTGCAAAAAGAAAGGGAAGATGGGATGTGTTA ATATTGTAGTGGCATGCACTTTCCAGCTCTCCATGGAGCTCTCTTTATATATGAGGTAATTGAATGTCACTATTCTGTACCATACAGCAGGTATTATCTA GCCAAGGTTATAATTACCATGAAGTGCTGTGGAATGTAGTAGGAGGCTTTTCACTGGG

BID.F1 (forward primer)
BID.R1 (reverse primer)
Single exon skipping

Frame 0

EGGSARGAIRRKRVVDRVRAPGRRCLGPDAPAP PRLEGGQQRFQPQG*VHHKPTGVWLPPKLF*QQL PQRAGRTGPRAASAGSPVGGLR*AAD*WQPQQP LPLGKNRGRF*KSRRHHPEYCQAPRPGRGQHGP *HPSGPGERPGPAAQEHQPVGGGPEQGPGHCPGA AAAGLP*RHGEGEDHAGAGPAAGQEGGQSHAVL AP*CLSHNSEFY*PEPTHLREELSQKWDGLNGQ FQKCDWLKLDVVTAV*LLPV*TEPWHVNSVPRED RLEDSCDFYFKDNVKLITHFKISTLIYLNENVH LHVFEWPSYHPHMNLHICKSTHGAFISTVQVPT *KLNWKAGFKEVETKYNFFGKKKLLFIKVQP*RM VLQQAVSCLRKARIREGTYPLQMKNSTQNRDYL S*Y*GTNNLPV*KTTWHRDSELKCCTQMMGSPA PVHRGLTFSTSFPLLESKRTTCGSKRCEGDLRAQ VSHCLFTKSGN*LHTLFLFP*HQDFAKDMKPRV PEATAMPRELAPW**L*TSNF*PYAQLIHASEV HLERTGF*A*KMKEQLDFLKIQQSGSLS*GQSKP SHLGSTKINKEKLSFRNSATLVCCSSGKLCPRN QFKSTTVDAGSISHTLL*TGNSCDHNMRPLETH MSKGTDGLMISSYQMLSCSLRV*NHQKGKTNSIL FSMQYIPR*GWLN*KWAKAYKY*KEVLPRSVEA TVWK*IFLTLRLFMFWSGLLKKFSNHNGNRLNH TAPTHPSIRQPL*SDSHWGRHFLPKGAVCSHHPE LMGKCS*AASDSGPPLSHTDSSTASSPASVSLE FIQ*ATIL*QVLV*ILPDQV*DSLGCKARPSPL LPKPTL*KDSIVSNTNVPVLSTSQPW*LGHILAV GGFCPVTQSLWI*FKGDFNSENVFSFLF*KYLN *VMEILLLNIKSFLLEKMLGVSESHVGHLVHSE GQIIPDLSS*DPALTAAILKASLKDHSTGA*FYC PATLLVVLAENWNIESFECYDLVFKTWSQTN*V WT*QRQLMM*NDTTCQGHSYHVDRKMSPLIIFK SHVFSLARCF*QKE*LTDLKFVPFP**RVDWPKG R*QEGRGARMTHSDQFSSAERQCRGSWNSHGKR KHMLLKMHDNLIIQITIQFRYF*DESI*ANTKL TLIKE*GRKRHF*SGGRNKWKTVIGKIQV*EKNN FFPLMLAKDPST*YSSPAGKHQGKQSYSKLCNR EDCTLTFFSWGKGTTSNL*MSKLFF*IVHSLTS FFWWCKEHSPQ*AIPLFNFPFGEVTAG*NHLESA ASL*LMTRPCKYP*EQFHSELTGKQQSGVGSCG VTIKCGRPDGVSVWGCRAEIQQPCKKKGKMGCV NIVVACTFQLSMELSLYMR*LNVTILYHTAGII* PRL*LP*SAVECSRRLFTG

Frame 1

KEEARGAP*GGSG*STVSARLGDAASARTRPRP RGWRVVNNGSSLRDECITNLLVFGFLQSCSDNS FRRELDALGHELPVLAPQWEGYDELQTDGNRSSH SRLGRIEADSESQEDIIRNIARHLAQVGDSMDR SIPPGLVNGLALQLRNTSRSEEDRNRDLATALE QLLQAYPRDMEKEKTMLVLALLLAKKVASHTPSL LRDVFHTTVNFINQNLRTYVRSLARNGMD*TDS SRSVTG*SSMWSQLYSCFQCRRSPGMSTAFLEK TGWKIAVTSILKTMLNL*PTLKYLH*YT*MKMSI YTYLNGLHIIHT*ICTSVNLHTVPLFPLCRFPL KN*IGKQVSRK*KQNTIFLVKKNYCLLKYNHRG WSYSRQYPV*GKQESEKEHTPYK*KIPLKIGTIY LNTKEPTIFLFKKPHGTEILN*SAALK*WEVRP QYTGA*LFQLRFLCWSQKEPLVVLKGVKVI*GP RSATVCLQNQVTNCIHFFSFHDIKTLLKT*SHGC QKLLRCPGS*PPGNSCKLPISSHTLSSSMPQKC IWREQVSKHKR*KSSWTF*KFSKVVPSLRDSQN QVT*VVPK*IRKSLALETVQHWSAVPVVSYVPGI SLKARQWMLGPYHTHCCEQETPVTTT*GHWRRI *VRALTDS*FLLTRCFPVL*EFKIIRKEKQTLY CSACNTYHARAGSIESGQKLTNTKKKCCRAVWRP LFGNKSS*HYDFSCFGVDF*KSFPTTMETG*TT LRLHIHPYVSHYNQILTGGGIFFPKELYAVITR N*WESVLKQQVTLDHLSHTLTLPQHLHLLLFHLN SYSRPPFYNRS*CKFFQTKSEIVLVVKQDRHPF SQSQHYKKIPLSQTQMFQS*ALPSHGDSGISWQ LVDFAL*HNPCGYDSKVTLIQRMCFPFCFKNI*I K*WKFFC*TSKAFSLKRCLVYLKVTWDIWCIQK VKSFLTSPLRTQH*LLRYSRLP*RITALAPSFT VQQLY*LC*LRIGI*SPLNVTILFSKPGVKPIKY GLNKDN**CEMIPPVKGIVTMWIER*VH*LSSN PMYSL*HVASDRKNNSQI*SLFLSPDEE*IGQK VDDRKEEEPE*PTVISSAVQSGSAEAHGTHTEKG NTCCLKCTIT*LSKLPFNSGIFEMKAFEPTLN* P*LRNEVGRGTFNLEEEINGRL**EKYRSRKKI IFFL*CWPRTPLHNTVLLLESIRANRVIPNSATG KTVL*PSFPGEREQLVIFKCQNFSSK*YIH*PV SSGGAKNTHHSEQYHYSISPLER*QRGRITWSQ LLVFN**QGLANTPENNFTLSSQGSSSLVWAPVV *PSNVEDLMVLVCGDAGLKFNSLAKRKGRWDVL IL*WHALSSSPWSSLYI*GN*MSLFCTIQQVLS SQGYNYHEVLWNVVGGFSL

Frame 2

RRKRAGRHKEEAGSRPCPRAWETLPRPGRARAP AAGGWSTTVPASGMSASQTYWCLASSKAVLTTA SAESWTHWATSCQCWLPSGRATMSCRLMATAAA TPAWEE*RQILKVKKTSSGILPGTSPRSGTAWTV ASLRAW*TAWPCSSGTPAGRRRTGTGTWPLPWS SCCRPTLETWRRRRPCWCWPCCWPRRWPVTRRP CSVMSFTQQ*ILLTRTYAPT*GA*PEMGWTERTV PEV*LAKARCGHSCIAASSVDGALACQQRS*RR QAGR*L*LLF*RQC*TYNPL*NIYINILE*KCP FTRI*MAFISSTHESAHL*IYTRCLYFHCAGSHL KIKLESRFQGSRNKIQFFW*KKITVY*STTIED GLTAGSILFEESKNQRRNIPLTNEKFHSK*GLS ILILRNQQSSCLKNHMAQRF*TKVLHSNDGKSGP STQGLDFFNFVSFVGVKKNHLWF*KV*R*FKGP GQPLFVYKIR*LTAYTFSLSMTSRLC*RHEATG ARSYCDAPGVSPLVIAVNFQFLAIRSAHPCLRSA SGENRFLSIKDERAVGLFKNSAKWFPLLGTVKT KSLR*YQNK*GKA*L*KQCNTGLLFQW*AMSQE SV*KHDSGCWVHITHIAVNRKLL*PQHEATGDAY E*GH*RTHDFFLPDAFLFFKSLKSSERKNKLYI VQHAIHTTLGLAQLKVGKSLQILKRSAAAQCGG HCLEINLPNTTTFHVLEWTFKKVFQPQWKQVEPH CAYTSIHTSAIIIRFSLGEAFSSQRSCMQSSPG TNGKVFLSSK*LWTTSLTH*LFHSIFTCFCFT* IHTVGHHFITGLSVNSSRPSLR*SWL*SKTVTPS PKANIIKRFHCLKHKCSSLEHFPAMVTRAYPGS WWILPCNTILVDMIQR*L*FRECVFLSVLKIFE LSDGNSFVKHQKLSP*KDAWCI*KSRGTFGAFRR SNHS*PLLLGPSINCCDTQGFLKGSQHWRLVLL SSNFISCAS*ELEYRVL*MLRSCFQNLESNQLS MDLTKTIDDVK*YHLSRA*LPCG*KDESTDYLQI PCILSSTLLLTERITHRFEVCSFPLMKSRLAKR *MTGRKRSQNDPQ*SVQQCRAAVQRLMELTRKK ETHAA*NAR*LNYPNYHSIPVFLR*KHLSQH*ID LN*GMR*EEALLIWRKK*MEDCNRKNTGLGKK* FFSFDAGQGPLYIIQFSCWKASGQTELFQTLQQ GRLYFDLLFLGKGNN**SLNVKTFLLNSTFINQF LLVVQRTLTTVSNTTIQFPLWRGDSGVESPGVS C*SLTDDKALQIPLRTISL*AHREAAVWCGLLW CNHQMWKT*WC*CVGMPG*NSTALQKEREDGMC* YCSGMHFPALHGALFIYEVIECHYSVPYSRYYL AKVIITMKCCGM**EAFHW