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Sequences

Sequence BID.hAug05

GAAGGAGGAAGCGCGCGGGGCGCCATAAGGAGGAAGCGGGTAGTCGACCGTGTCCGCGCGCCTGGGAGACGCTGCCTCGGCCCGGACGCGCCCGCGCCCC CGCGGCTGGAGGGTGATTCTGAAAGTCAAGAAGACATCATCCGGAATATTGCCAGGCACCTCGCCCAGGTCGGGGACAGCATGGACCGTAGCATCCCTCC GGGCCTGGTGAACGGCCTGGCCCTGCAGCTCAGGAACACCAGCCGGTCGGAGGAGGACCGGAACAGGGACCTGGCCACTGCCCTGGAGCAGCTGCTGCAG GCCTACCCTAGAGACATGGAGAAGGAGAAGACCATGCTGGTGCTGGCCCTGCTGCTGGCCAAGAAGGTGGCCAGTCACACGCCGTCCTTGCTCCGTGATG TCTTTCACACAACAGTGAATTTTATTAACCAGAACCTACGCACCTACGTGAGGAGCTTAGCCAGAAATGGGATGGACTGAACGGACAGTTCCAGAAGTGT GACTGGCTAAAGCTCGATGTGGTCACAGCTGTATAGCTGCTTCCAGTGTAGACGGAGCCCTGGCATGTCAACAGCGTTCCTAGAGAAGACAGGCTGGAAG ATAGCTGTGACTTCTATTTTAAAGACAATGTTAAACTTATAACCCACTTTAAAATATCTACATTAATATACTTGAATGAAAATGTCCATTTACACGTATT TGAATGGCCTTCATATCATCCACACATGAATCTGCACATCTGTAAATCTACACACGGTGCCTTTATTTCCACTGTGCAGGTTCCCACTTAAAAATTAAAT TGGAAAGCAGGTTTCAAGGAAGTAGAAACAAAATACAATTTTTTTGGTAAAAAAAAATTACTGTTTATTAAAGTACAACCATAGAGGATGGTCTTACAGC AGGCAGTATCCTGTTTGAGGAAAGCAAGAATCAGAGAAGGAACATACCCCTTACAAATGAAAAATTCCACTCAAAATAGGGACTATCTATCTTAATACTA AGGAACCAACAATCTTCCTGTTTAAAAAACCACATGGCACAGAGATTCTGAACTAAAGTGCTGCACTCAAATGATGGGAAGTCCGGCCCCAGTACACAGG GGCTTGACTTTTTCAACTTCGTTTCCTTTGTTGGAGTCAAAAAGAACCACTTGTGGTTCTAAAAGGTGTGAAGGTGATTTAAGGGCCCAGGTCAGCCACT GTTTGTTTACAAAATCAGGTAACTAACTGCATACACTTTTTCTCTTTCCATGACATCAAGACTTTGCTAAAGACATGAAGCCACGGGTGCCAGAAGCTAC TGCGATGCCCCGGGAGTTAGCCCCCTGGTAATAGCTGTAAACTTCCAATTTCTAGCCATACGCTCAGCTCATCCATGCCTCAGAAGTGCATCTGGAGAGA ACAGGTTTCTAAGCATAAAAGATGAAAGAGCAGTTGGACTTTTTAAAAATTCAGCAAAGTGGTTCCCTCTCTTAGGGACAGTCAAAACCAAGTCACTTAG GTAGTACCAAAATAAATAAGGAAAAGCTTAGCTTTAGAAACAGTGCAACACTGGTCTGCTGTTCCAGTGGTAAGCTATGTCCCAGGAATCAGTTTAAAAG CACGACAGTGGATGCTGGGTCCATATCACACACATTGCTGTGAACAGGAAACTCCTGTGACCACAACATGAGGCCACTGGAGACGCATATGAGTAAGGGC ACTGACGGACTCATGATTTCTTCTTACCAGATGCTTTCCTGTTCTTTAAGAGTTTAAAATCATCAGAAAGGAAAAACAAACTCTATATTGTTCAGCATGC AATACATACCACGCTAGGGCTGGCTCAATTGAAAGTGGGCAAAAGCTTACAAATACTAAAAAGAAGTGCTGCCGCGCAGTGTGGAGGCCACTGTTTGGAA ATAAATCTTCCTAACACTACGACTTTTCATGTTTTGGAGTGGACTTTTAAAAAAGTTTTCCAACCACAATGGAAACAGGTTGAACCACACTGCGCCTACA CATCCATCCATACGTCAGCCATTATAATCAGATTCTCACTGGGGGAGGCATTTTCTTCCCAAAGGAGCTGTATGCAGTCATCACCCGGAACTAATGGGAA AGTGTTCTTAAGCAGCAAGTGACTCTGGACCACCTCTCTCACACACTGACTCTTCCACAGCATCTTCACCTGCTTCTGTTTCACTTGAATTCATACAGTA GGCCACCATTTTATAACAGGTCTTAGTGTAAATTCTTCCAGACCAAGTCTGAGATAGTCTTGGTTGTAAAGCAAGACCGTCACCCCTTCTCCCAAAGCCA ACATTATAAAAAGATTCCATTGTCTCAAACACAAATGTTCCAGTCTTGAGCACTTCCCAGCCATGGTGACTCGGGCATATCCTGGCAGTTGGTGGATTTT GCCCTGTAACACAATCCTTGTGGATATGATTCAAAGGTGACTTTAATTCAGAGAATGTGTTTTCCTTTCTGTTTTAAAAATATTTGAATTAAGTGATGGA AATTCTTTTGTTAAACATCAAAAGCTTTCTCCTTGAAAAGATGCTTGGTGTATCTGAAAGTCACGTGGGACATTTGGTGCATTCAGAAGGTCAAATCATT CCTGACCTCTCCTCTTAGGACCCAGCATTAACTGCTGCGATACTCAAGGCTTCCTTAAAGGATCACAGCACTGGCGCCTAGTTTTACTGTCCAGCAACTT TATTAGTTGTGCTAGCTGAGAATTGGAATATAGAGTCCTTTGAATGTTACGATCTTGTTTTCAAAACCTGGAGTCAAACCAATTAAGTATGGACTTAACA AAGACAATTGATGATGTGAAATGATACCACCTGTCAAGGGCATAGTTACCATGTGGATAGAAAGATGAGTCCACTGATTATCTTCAAATCCCATGTATTC TCTCTAGCACGTTGCTTCTGACAGAAAGAATAACTCACAGATTTGAAGTTTGTTCCTTTCCCCTGATGAAGAGTAGATTGGCCAAAAGGTAGATGACAGG AAGGAAGAGGAGCCAGAATGACCCACAGTGATCAGTTCAGCAGTGCAGAGCGGCAGTGCAGAGGCTCATGGAACTCACACGGAAAAAGGAAACACATGCT GCTTAAAATGCACGATAACTTAATTATCCAAATTACCATTCAATTCCGGTATTTTTGAGATGAAAGCATTTGAGCCAACACTAAATTGACCTTAATTAAG GAATGAGGTAGGAAGAGGCACTTTTAATCTGGAGGAAGAAATAAATGGAAGACTGTAATAGGAAAAATACAGGTCTAGGAAAAAAATAATTTTTTTCCTT TGATGCTGGCCAAGGACCCCTCTACATAATACAGTTCTCCTGCTGGAAAGCATCAGGGCAAACAGAGTTATTCCAAACTCTGCAACAGGGAAGACTGTAC TTTGACCTTCTTTTCCTGGGGAAAGGGAACAACTAGTAATCTTTAAATGTCAAAACTTTTCTTCTAAATAGTACATTCATTAACCAGTTTCTTCTGGTGG TGCAAAGAACACTCACCACAGTGAGCAATACCACTATTCAATTTCCCCTTTGGAGAGGTGACAGCGGGGTAGAATCACCTGGAGTCAGCTGCTAGTCTTT AACTGATGACAAGGCCTTGCAAATACCCCTGAGAACAATTTCACTCTGAGCTCACAGGGAAGCAGCAGTCTGGTGTGGGCTCCTGTGGTGTAACCATCAA ATGTGGAAGACCTGATGGTGTTAGTGTGTGGGGATGCCGGGCTGAAATTCAACAGCCTTGCAAAAAGAAAGGGAAGATGGGATGTGTTAATATTGTAGTG GCATGCACTTTCCAGCTCTCCATGGAGCTCTCTTTATATATGAGGTAATTGAATGTCACTATTCTGTACCATACAGCAGGTATTATCTAGCCAAGGTTAT AATTACCATGAAGTGCTGTGGAATGTAGTAGGAGGCTTTTCACTGGG

BID.F1 (forward primer)
BID.R1 (reverse primer)
Multiple exon skipping

Frame 0

EGGSARGAIRRKRVVDRVRAPGRRCLGPDAPAP PRLEGDSESQEDIIRNIARHLAQVGDSMDRSIPP GLVNGLALQLRNTSRSEEDRNRDLATALEQLLQ AYPRDMEKEKTMLVLALLLAKKVASHTPSLLRD VFHTTVNFINQNLRTYVRSLARNGMD*TDSSRSV TG*SSMWSQLYSCFQCRRSPGMSTAFLEKTGWK IAVTSILKTMLNL*PTLKYLH*YT*MKMSIYTY LNGLHIIHT*ICTSVNLHTVPLFPLCRFPLKN*I GKQVSRK*KQNTIFLVKKNYCLLKYNHRGWSYS RQYPV*GKQESEKEHTPYK*KIPLKIGTIYLNT KEPTIFLFKKPHGTEILN*SAALK*WEVRPQYTG A*LFQLRFLCWSQKEPLVVLKGVKVI*GPRSAT VCLQNQVTNCIHFFSFHDIKTLLKT*SHGCQKL LRCPGS*PPGNSCKLPISSHTLSSSMPQKCIWRE QVSKHKR*KSSWTF*KFSKVVPSLRDSQNQVT* VVPK*IRKSLALETVQHWSAVPVVSYVPGISLK ARQWMLGPYHTHCCEQETPVTTT*GHWRRI*VRA LTDS*FLLTRCFPVL*EFKIIRKEKQTLYCSAC NTYHARAGSIESGQKLTNTKKKCCRAVWRPLFG NKSS*HYDFSCFGVDF*KSFPTTMETG*TTLRLH IHPYVSHYNQILTGGGIFFPKELYAVITRN*WE SVLKQQVTLDHLSHTLTLPQHLHLLLFHLNSYS RPPFYNRS*CKFFQTKSEIVLVVKQDRHPFSQSQ HYKKIPLSQTQMFQS*ALPSHGDSGISWQLVDF AL*HNPCGYDSKVTLIQRMCFPFCFKNI*IK*W KFFC*TSKAFSLKRCLVYLKVTWDIWCIQKVKSF LTSPLRTQH*LLRYSRLP*RITALAPSFTVQQL Y*LC*LRIGI*SPLNVTILFSKPGVKPIKYGLN KDN**CEMIPPVKGIVTMWIER*VH*LSSNPMYS L*HVASDRKNNSQI*SLFLSPDEE*IGQKVDDR KEEEPE*PTVISSAVQSGSAEAHGTHTEKGNTC CLKCTIT*LSKLPFNSGIFEMKAFEPTLN*P*LR NEVGRGTFNLEEEINGRL**EKYRSRKKIIFFL *CWPRTPLHNTVLLLESIRANRVIPNSATGKTV L*PSFPGEREQLVIFKCQNFSSK*YIH*PVSSGG AKNTHHSEQYHYSISPLER*QRGRITWSQLLVF N**QGLANTPENNFTLSSQGSSSLVWAPVV*PS NVEDLMVLVCGDAGLKFNSLAKRKGRWDVLIL*W HALSSSPWSSLYI*GN*MSLFCTIQQVLSSQGY NYHEVLWNVVGGFSL

Frame 1

KEEARGAP*GGSG*STVSARLGDAASARTRPRP RGWRVILKVKKTSSGILPGTSPRSGTAWTVASL RAW*TAWPCSSGTPAGRRRTGTGTWPLPWSSCCR PTLETWRRRRPCWCWPCCWPRRWPVTRRPCSVM SFTQQ*ILLTRTYAPT*GA*PEMGWTERTVPEV *LAKARCGHSCIAASSVDGALACQQRS*RRQAGR *L*LLF*RQC*TYNPL*NIYINILE*KCPFTRI *MAFISSTHESAHL*IYTRCLYFHCAGSHLKIK LESRFQGSRNKIQFFW*KKITVY*STTIEDGLTA GSILFEESKNQRRNIPLTNEKFHSK*GLSILIL RNQQSSCLKNHMAQRF*TKVLHSNDGKSGPSTQ GLDFFNFVSFVGVKKNHLWF*KV*R*FKGPGQPL FVYKIR*LTAYTFSLSMTSRLC*RHEATGARSY CDAPGVSPLVIAVNFQFLAIRSAHPCLRSASGE NRFLSIKDERAVGLFKNSAKWFPLLGTVKTKSLR *YQNK*GKA*L*KQCNTGLLFQW*AMSQESV*K HDSGCWVHITHIAVNRKLL*PQHEATGDAYE*G H*RTHDFFLPDAFLFFKSLKSSERKNKLYIVQHA IHTTLGLAQLKVGKSLQILKRSAAAQCGGHCLE INLPNTTTFHVLEWTFKKVFQPQWKQVEPHCAY TSIHTSAIIIRFSLGEAFSSQRSCMQSSPGTNGK VFLSSK*LWTTSLTH*LFHSIFTCFCFT*IHTV GHHFITGLSVNSSRPSLR*SWL*SKTVTPSPKA NIIKRFHCLKHKCSSLEHFPAMVTRAYPGSWWIL PCNTILVDMIQR*L*FRECVFLSVLKIFELSDG NSFVKHQKLSP*KDAWCI*KSRGTFGAFRRSNH S*PLLLGPSINCCDTQGFLKGSQHWRLVLLSSNF ISCAS*ELEYRVL*MLRSCFQNLESNQLSMDLT KTIDDVK*YHLSRA*LPCG*KDESTDYLQIPCI LSSTLLLTERITHRFEVCSFPLMKSRLAKR*MTG RKRSQNDPQ*SVQQCRAAVQRLMELTRKKETHA A*NAR*LNYPNYHSIPVFLR*KHLSQH*IDLN* GMR*EEALLIWRKK*MEDCNRKNTGLGKK*FFSF DAGQGPLYIIQFSCWKASGQTELFQTLQQGRLY FDLLFLGKGNN**SLNVKTFLLNSTFINQFLLV VQRTLTTVSNTTIQFPLWRGDSGVESPGVSC*SL TDDKALQIPLRTISL*AHREAAVWCGLLWCNHQ MWKT*WC*CVGMPG*NSTALQKEREDGMC*YCS GMHFPALHGALFIYEVIECHYSVPYSRYYLAKVI ITMKCCGM**EAFHW

Frame 2

RRKRAGRHKEEAGSRPCPRAWETLPRPGRARAP AAGG*F*KSRRHHPEYCQAPRPGRGQHGP*HPS GPGERPGPAAQEHQPVGGGPEQGPGHCPGAAAA GLP*RHGEGEDHAGAGPAAGQEGGQSHAVLAP*C LSHNSEFY*PEPTHLREELSQKWDGLNGQFQKC DWLKLDVVTAV*LLPV*TEPWHVNSVPREDRLE DSCDFYFKDNVKLITHFKISTLIYLNENVHLHVF EWPSYHPHMNLHICKSTHGAFISTVQVPT*KLN WKAGFKEVETKYNFFGKKKLLFIKVQP*RMVLQ QAVSCLRKARIREGTYPLQMKNSTQNRDYLS*Y* GTNNLPV*KTTWHRDSELKCCTQMMGSPAPVHR GLTFSTSFPLLESKRTTCGSKRCEGDLRAQVSH CLFTKSGN*LHTLFLFP*HQDFAKDMKPRVPEAT AMPRELAPW**L*TSNF*PYAQLIHASEVHLER TGF*A*KMKEQLDFLKIQQSGSLS*GQSKPSHL GSTKINKEKLSFRNSATLVCCSSGKLCPRNQFKS TTVDAGSISHTLL*TGNSCDHNMRPLETHMSKG TDGLMISSYQMLSCSLRV*NHQKGKTNSILFSM QYIPR*GWLN*KWAKAYKY*KEVLPRSVEATVWK *IFLTLRLFMFWSGLLKKFSNHNGNRLNHTAPT HPSIRQPL*SDSHWGRHFLPKGAVCSHHPELMG KCS*AASDSGPPLSHTDSSTASSPASVSLEFIQ* ATIL*QVLV*ILPDQV*DSLGCKARPSPLLPKP TL*KDSIVSNTNVPVLSTSQPW*LGHILAVGGF CPVTQSLWI*FKGDFNSENVFSFLF*KYLN*VME ILLLNIKSFLLEKMLGVSESHVGHLVHSEGQII PDLSS*DPALTAAILKASLKDHSTGA*FYCPAT LLVVLAENWNIESFECYDLVFKTWSQTN*VWT*Q RQLMM*NDTTCQGHSYHVDRKMSPLIIFKSHVF SLARCF*QKE*LTDLKFVPFP**RVDWPKGR*Q EGRGARMTHSDQFSSAERQCRGSWNSHGKRKHML LKMHDNLIIQITIQFRYF*DESI*ANTKLTLIK E*GRKRHF*SGGRNKWKTVIGKIQV*EKNNFFP LMLAKDPST*YSSPAGKHQGKQSYSKLCNREDCT LTFFSWGKGTTSNL*MSKLFF*IVHSLTSFFWW CKEHSPQ*AIPLFNFPFGEVTAG*NHLESAASL *LMTRPCKYP*EQFHSELTGKQQSGVGSCGVTIK CGRPDGVSVWGCRAEIQQPCKKKGKMGCVNIVV ACTFQLSMELSLYMR*LNVTILYHTAGII*PRL *LP*SAVECSRRLFTG

Sequence BID.fAug05

GAAGGAGGAAGCGCGCGGGGCGCCATAAGGAGGAAGCGGGTAGTCGACCGTGTCCGCGCGCCTGGGAGACGCTGCCTCGGCCCGGACGCGCCCGCGCCCC CGCGGCTGGAGGGTGGTCGCCACTGGGACACTGTGAACCAGGAGTGAGTCGGAGCTGCCGCGCTGCCCAGGCCATGGACTGTGAGGTCAACAACGGTTCC AGCCTCAGGGATGAGTGCATCACAAACCTACTGGTGTTTGGCTTCCTCCAAAGCTGTTCTGACAACAGCTTCCGCAGAGAGCTGGACGCACTGGGCCACG AGCTGCCAGTGCTGGCTCCCCAGTGGGAGGGCTACGATGAGCTGCAGACTGATGGCAACCGCAGCAGCCACTCCCGCTTGGGAAGAATAGAGGCAGGGGC GTCAGACAATAACACAGCAAGTGCTGAGGAAGAAACGGAGGCGGCAGGGAGCGTGGCAGTTGAGCGTGGCCTTCATGGAGCTGCGACAGTGATTCTGAAA GTCAAGAAGACATCATCCGGAATATTGCCAGGCACCTCGCCCAGGTCGGGGACAGCATGGACCGTAGCATCCCTCCGGGCCTGGTGAACGGCCTGGCCCT GCAGCTCAGGAACACCAGCCGGTCGGAGGAGGACCGGAACAGGGACCTGGCCACTGCCCTGGAGCAGCTGCTGCAGGCCTACCCTAGAGACATGGAGAAG GAGAAGACCATGCTGGTGCTGGCCCTGCTGCTGGCCAAGAAGGTGGCCAGTCACACGCCGTCCTTGCTCCGTGATGTCTTTCACACAACAGTGAATTTTA TTAACCAGAACCTACGCACCTACGTGAGGAGCTTAGCCAGAAATGGGATGGACTGAACGGACAGTTCCAGAAGTGTGACTGGCTAAAGCTCGATGTGGTC ACAGCTGTATAGCTGCTTCCAGTGTAGACGGAGCCCTGGCATGTCAACAGCGTTCCTAGAGAAGACAGGCTGGAAGATAGCTGTGACTTCTATTTTAAAG ACAATGTTAAACTTATAACCCACTTTAAAATATCTACATTAATATACTTGAATGAAAATGTCCATTTACACGTATTTGAATGGCCTTCATATCATCCACA CATGAATCTGCACATCTGTAAATCTACACACGGTGCCTTTATTTCCACTGTGCAGGTTCCCACTTAAAAATTAAATTGGAAAGCAGGTTTCAAGGAAGTA GAAACAAAATACAATTTTTTTGGTAAAAAAAAATTACTGTTTATTAAAGTACAACCATAGAGGATGGTCTTACAGCAGGCAGTATCCTGTTTGAGGAAAG CAAGAATCAGAGAAGGAACATACCCCTTACAAATGAAAAATTCCACTCAAAATAGGGACTATCTATCTTAATACTAAGGAACCAACAATCTTCCTGTTTA AAAAACCACATGGCACAGAGATTCTGAACTAAAGTGCTGCACTCAAATGATGGGAAGTCCGGCCCCAGTACACAGGGGCTTGACTTTTTCAACTTCGTTT CCTTTGTTGGAGTCAAAAAGAACCACTTGTGGTTCTAAAAGGTGTGAAGGTGATTTAAGGGCCCAGGTCAGCCACTGTTTGTTTACAAAATCAGGTAACT AACTGCATACACTTTTTCTCTTTCCATGACATCAAGACTTTGCTAAAGACATGAAGCCACGGGTGCCAGAAGCTACTGCGATGCCCCGGGAGTTAGCCCC CTGGTAATAGCTGTAAACTTCCAATTTCTAGCCATACGCTCAGCTCATCCATGCCTCAGAAGTGCATCTGGAGAGAACAGGTTTCTAAGCATAAAAGATG AAAGAGCAGTTGGACTTTTTAAAAATTCAGCAAAGTGGTTCCCTCTCTTAGGGACAGTCAAAACCAAGTCACTTAGGTAGTACCAAAATAAATAAGGAAA AGCTTAGCTTTAGAAACAGTGCAACACTGGTCTGCTGTTCCAGTGGTAAGCTATGTCCCAGGAATCAGTTTAAAAGCACGACAGTGGATGCTGGGTCCAT ATCACACACATTGCTGTGAACAGGAAACTCCTGTGACCACAACATGAGGCCACTGGAGACGCATATGAGTAAGGGCACTGACGGACTCATGATTTCTTCT TACCAGATGCTTTCCTGTTCTTTAAGAGTTTAAAATCATCAGAAAGGAAAAACAAACTCTATATTGTTCAGCATGCAATACATACCACGCTAGGGCTGGC TCAATTGAAAGTGGGCAAAAGCTTACAAATACTAAAAAGAAGTGCTGCCGCGCAGTGTGGAGGCCACTGTTTGGAAATAAATCTTCCTAACACTACGACT TTTCATGTTTTGGAGTGGACTTTTAAAAAAGTTTTCCAACCACAATGGAAACAGGTTGAACCACACTGCGCCTACACATCCATCCATACGTCAGCCATTA TAATCAGATTCTCACTGGGGGAGGCATTTTCTTCCCAAAGGAGCTGTATGCAGTCATCACCCGGAACTAATGGGAAAGTGTTCTTAAGCAGCAAGTGACT CTGGACCACCTCTCTCACACACTGACTCTTCCACAGCATCTTCACCTGCTTCTGTTTCACTTGAATTCATACAGTAGGCCACCATTTTATAACAGGTCTT AGTGTAAATTCTTCCAGACCAAGTCTGAGATAGTCTTGGTTGTAAAGCAAGACCGTCACCCCTTCTCCCAAAGCCAACATTATAAAAAGATTCCATTGTC TCAAACACAAATGTTCCAGTCTTGAGCACTTCCCAGCCATGGTGACTCGGGCATATCCTGGCAGTTGGTGGATTTTGCCCTGTAACACAATCCTTGTGGA TATGATTCAAAGGTGACTTTAATTCAGAGAATGTGTTTTCCTTTCTGTTTTAAAAATATTTGAATTAAGTGATGGAAATTCTTTTGTTAAACATCAAAAG CTTTCTCCTTGAAAAGATGCTTGGTGTATCTGAAAGTCACGTGGGACATTTGGTGCATTCAGAAGGTCAAATCATTCCTGACCTCTCCTCTTAGGACCCA GCATTAACTGCTGCGATACTCAAGGCTTCCTTAAAGGATCACAGCACTGGCGCCTAGTTTTACTGTCCAGCAACTTTATTAGTTGTGCTAGCTGAGAATT GGAATATAGAGTCCTTTGAATGTTACGATCTTGTTTTCAAAACCTGGAGTCAAACCAATTAAGTATGGACTTAACAAAGACAATTGATGATGTGAAATGA TACCACCTGTCAAGGGCATAGTTACCATGTGGATAGAAAGATGAGTCCACTGATTATCTTCAAATCCCATGTATTCTCTCTAGCACGTTGCTTCTGACAG AAAGAATAACTCACAGATTTGAAGTTTGTTCCTTTCCCCTGATGAAGAGTAGATTGGCCAAAAGGTAGATGACAGGAAGGAAGAGGAGCCAGAATGACCC ACAGTGATCAGTTCAGCAGTGCAGAGCGGCAGTGCAGAGGCTCATGGAACTCACACGGAAAAAGGAAACACATGCTGCTTAAAATGCACGATAACTTAAT TATCCAAATTACCATTCAATTCCGGTATTTTTGAGATGAAAGCATTTGAGCCAACACTAAATTGACCTTAATTAAGGAATGAGGTAGGAAGAGGCACTTT TAATCTGGAGGAAGAAATAAATGGAAGACTGTAATAGGAAAAATACAGGTCTAGGAAAAAAATAATTTTTTTCCTTTGATGCTGGCCAAGGACCCCTCTA CATAATACAGTTCTCCTGCTGGAAAGCATCAGGGCAAACAGAGTTATTCCAAACTCTGCAACAGGGAAGACTGTACTTTGACCTTCTTTTCCTGGGGAAA GGGAACAACTAGTAATCTTTAAATGTCAAAACTTTTCTTCTAAATAGTACATTCATTAACCAGTTTCTTCTGGTGGTGCAAAGAACACTCACCACAGTGA GCAATACCACTATTCAATTTCCCCTTTGGAGAGGTGACAGCGGGGTAGAATCACCTGGAGTCAGCTGCTAGTCTTTAACTGATGACAAGGCCTTGCAAAT ACCCCTGAGAACAATTTCACTCTGAGCTCACAGGGAAGCAGCAGTCTGGTGTGGGCTCCTGTGGTGTAACCATCAAATGTGGAAGACCTGATGGTGTTAG TGTGTGGGGATGCCGGGCTGAAATTCAACAGCCTTGCAAAAAGAAAGGGAAGATGGGATGTGTTAATATTGTAGTGGCATGCACTTTCCAGCTCTCCATG GAGCTCTCTTTATATATGAGGTAATTGAATGTCACTATTCTGTACCATACAGCAGGTATTATCTAGCCAAGGTTATAATTACCATGAAGTGCTGTGGAAT GTAGTAGGAGGCTTTTCACTGGG

BID.F1 (forward primer)
BID.R1 (reverse primer)
Multiple exon skipping

Frame 0

EGGSARGAIRRKRVVDRVRAPGRRCLGPDAPAP PRLEGGRHWDTVNQE*VGAAALPRPWTVRSTTVP ASGMSASQTYWCLASSKAVLTTASAESWTHWAT SCQCWLPSGRATMSCRLMATAAATPAWEE*RQG RQTITQQVLRKKRRRQGAWQLSVAFMELRQ*F*K SRRHHPEYCQAPRPGRGQHGP*HPSGPGERPGP AAQEHQPVGGGPEQGPGHCPGAAAAGLP*RHGE GEDHAGAGPAAGQEGGQSHAVLAP*CLSHNSEFY *PEPTHLREELSQKWDGLNGQFQKCDWLKLDVV TAV*LLPV*TEPWHVNSVPREDRLEDSCDFYFK DNVKLITHFKISTLIYLNENVHLHVFEWPSYHPH MNLHICKSTHGAFISTVQVPT*KLNWKAGFKEV ETKYNFFGKKKLLFIKVQP*RMVLQQAVSCLRK ARIREGTYPLQMKNSTQNRDYLS*Y*GTNNLPV* KTTWHRDSELKCCTQMMGSPAPVHRGLTFSTSF PLLESKRTTCGSKRCEGDLRAQVSHCLFTKSGN *LHTLFLFP*HQDFAKDMKPRVPEATAMPRELAP W**L*TSNF*PYAQLIHASEVHLERTGF*A*KM KEQLDFLKIQQSGSLS*GQSKPSHLGSTKINKE KLSFRNSATLVCCSSGKLCPRNQFKSTTVDAGSI SHTLL*TGNSCDHNMRPLETHMSKGTDGLMISS YQMLSCSLRV*NHQKGKTNSILFSMQYIPR*GW LN*KWAKAYKY*KEVLPRSVEATVWK*IFLTLRL FMFWSGLLKKFSNHNGNRLNHTAPTHPSIRQPL *SDSHWGRHFLPKGAVCSHHPELMGKCS*AASD SGPPLSHTDSSTASSPASVSLEFIQ*ATIL*QVL V*ILPDQV*DSLGCKARPSPLLPKPTL*KDSIV SNTNVPVLSTSQPW*LGHILAVGGFCPVTQSLW I*FKGDFNSENVFSFLF*KYLN*VMEILLLNIKS FLLEKMLGVSESHVGHLVHSEGQIIPDLSS*DP ALTAAILKASLKDHSTGA*FYCPATLLVVLAEN WNIESFECYDLVFKTWSQTN*VWT*QRQLMM*ND TTCQGHSYHVDRKMSPLIIFKSHVFSLARCF*Q KE*LTDLKFVPFP**RVDWPKGR*QEGRGARMT HSDQFSSAERQCRGSWNSHGKRKHMLLKMHDNLI IQITIQFRYF*DESI*ANTKLTLIKE*GRKRHF *SGGRNKWKTVIGKIQV*EKNNFFPLMLAKDPS T*YSSPAGKHQGKQSYSKLCNREDCTLTFFSWGK GTTSNL*MSKLFF*IVHSLTSFFWWCKEHSPQ* AIPLFNFPFGEVTAG*NHLESAASL*LMTRPCK YP*EQFHSELTGKQQSGVGSCGVTIKCGRPDGVS VWGCRAEIQQPCKKKGKMGCVNIVVACTFQLSM ELSLYMR*LNVTILYHTAGII*PRL*LP*SAVE CSRRLFTG

Frame 1

KEEARGAP*GGSG*STVSARLGDAASARTRPRP RGWRVVATGTL*TRSESELPRCPGHGL*GQQRF QPQG*VHHKPTGVWLPPKLF*QQLPQRAGRTGPR AASAGSPVGGLR*AAD*WQPQQPLPLGKNRGRG VRQ*HSKC*GRNGGGRERGS*AWPSWSCDSDSE SQEDIIRNIARHLAQVGDSMDRSIPPGLVNGLAL QLRNTSRSEEDRNRDLATALEQLLQAYPRDMEK EKTMLVLALLLAKKVASHTPSLLRDVFHTTVNF INQNLRTYVRSLARNGMD*TDSSRSVTG*SSMWS QLYSCFQCRRSPGMSTAFLEKTGWKIAVTSILK TMLNL*PTLKYLH*YT*MKMSIYTYLNGLHIIH T*ICTSVNLHTVPLFPLCRFPLKN*IGKQVSRK* KQNTIFLVKKNYCLLKYNHRGWSYSRQYPV*GK QESEKEHTPYK*KIPLKIGTIYLNTKEPTIFLF KKPHGTEILN*SAALK*WEVRPQYTGA*LFQLRF LCWSQKEPLVVLKGVKVI*GPRSATVCLQNQVT NCIHFFSFHDIKTLLKT*SHGCQKLLRCPGS*P PGNSCKLPISSHTLSSSMPQKCIWREQVSKHKR* KSSWTF*KFSKVVPSLRDSQNQVT*VVPK*IRK SLALETVQHWSAVPVVSYVPGISLKARQWMLGP YHTHCCEQETPVTTT*GHWRRI*VRALTDS*FLL TRCFPVL*EFKIIRKEKQTLYCSACNTYHARAG SIESGQKLTNTKKKCCRAVWRPLFGNKSS*HYD FSCFGVDF*KSFPTTMETG*TTLRLHIHPYVSHY NQILTGGGIFFPKELYAVITRN*WESVLKQQVT LDHLSHTLTLPQHLHLLLFHLNSYSRPPFYNRS *CKFFQTKSEIVLVVKQDRHPFSQSQHYKKIPLS QTQMFQS*ALPSHGDSGISWQLVDFAL*HNPCG YDSKVTLIQRMCFPFCFKNI*IK*WKFFC*TSK AFSLKRCLVYLKVTWDIWCIQKVKSFLTSPLRTQ H*LLRYSRLP*RITALAPSFTVQQLY*LC*LRI GI*SPLNVTILFSKPGVKPIKYGLNKDN**CEM IPPVKGIVTMWIER*VH*LSSNPMYSL*HVASDR KNNSQI*SLFLSPDEE*IGQKVDDRKEEEPE*P TVISSAVQSGSAEAHGTHTEKGNTCCLKCTIT* LSKLPFNSGIFEMKAFEPTLN*P*LRNEVGRGTF NLEEEINGRL**EKYRSRKKIIFFL*CWPRTPL HNTVLLLESIRANRVIPNSATGKTVL*PSFPGE REQLVIFKCQNFSSK*YIH*PVSSGGAKNTHHSE QYHYSISPLER*QRGRITWSQLLVFN**QGLAN TPENNFTLSSQGSSSLVWAPVV*PSNVEDLMVL VCGDAGLKFNSLAKRKGRWDVLIL*WHALSSSPW SSLYI*GN*MSLFCTIQQVLSSQGYNYHEVLWN VVGGFSL

Frame 2

RRKRAGRHKEEAGSRPCPRAWETLPRPGRARAP AAGGWSPLGHCEPGVSRSCRAAQAMDCEVNNGS SLRDECITNLLVFGFLQSCSDNSFRRELDALGH ELPVLAPQWEGYDELQTDGNRSSHSRLGRIEAGA SDNNTASAEEETEAAGSVAVERGLHGAATVILK VKKTSSGILPGTSPRSGTAWTVASLRAW*TAWP CSSGTPAGRRRTGTGTWPLPWSSCCRPTLETWRR RRPCWCWPCCWPRRWPVTRRPCSVMSFTQQ*IL LTRTYAPT*GA*PEMGWTERTVPEV*LAKARCG HSCIAASSVDGALACQQRS*RRQAGR*L*LLF*R QC*TYNPL*NIYINILE*KCPFTRI*MAFISST HESAHL*IYTRCLYFHCAGSHLKIKLESRFQGS RNKIQFFW*KKITVY*STTIEDGLTAGSILFEES KNQRRNIPLTNEKFHSK*GLSILILRNQQSSCL KNHMAQRF*TKVLHSNDGKSGPSTQGLDFFNFV SFVGVKKNHLWF*KV*R*FKGPGQPLFVYKIR*L TAYTFSLSMTSRLC*RHEATGARSYCDAPGVSP LVIAVNFQFLAIRSAHPCLRSASGENRFLSIKD ERAVGLFKNSAKWFPLLGTVKTKSLR*YQNK*GK A*L*KQCNTGLLFQW*AMSQESV*KHDSGCWVH ITHIAVNRKLL*PQHEATGDAYE*GH*RTHDFF LPDAFLFFKSLKSSERKNKLYIVQHAIHTTLGLA QLKVGKSLQILKRSAAAQCGGHCLEINLPNTTT FHVLEWTFKKVFQPQWKQVEPHCAYTSIHTSAI IIRFSLGEAFSSQRSCMQSSPGTNGKVFLSSK*L WTTSLTH*LFHSIFTCFCFT*IHTVGHHFITGL SVNSSRPSLR*SWL*SKTVTPSPKANIIKRFHC LKHKCSSLEHFPAMVTRAYPGSWWILPCNTILVD MIQR*L*FRECVFLSVLKIFELSDGNSFVKHQK LSP*KDAWCI*KSRGTFGAFRRSNHS*PLLLGP SINCCDTQGFLKGSQHWRLVLLSSNFISCAS*EL EYRVL*MLRSCFQNLESNQLSMDLTKTIDDVK* YHLSRA*LPCG*KDESTDYLQIPCILSSTLLLT ERITHRFEVCSFPLMKSRLAKR*MTGRKRSQNDP Q*SVQQCRAAVQRLMELTRKKETHAA*NAR*LN YPNYHSIPVFLR*KHLSQH*IDLN*GMR*EEAL LIWRKK*MEDCNRKNTGLGKK*FFSFDAGQGPLY IIQFSCWKASGQTELFQTLQQGRLYFDLLFLGK GNN**SLNVKTFLLNSTFINQFLLVVQRTLTTV SNTTIQFPLWRGDSGVESPGVSC*SLTDDKALQI PLRTISL*AHREAAVWCGLLWCNHQMWKT*WC* CVGMPG*NSTALQKEREDGMC*YCSGMHFPALH GALFIYEVIECHYSVPYSRYYLAKVIITMKCCGM **EAFHW