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Sequences

Sequence IKBKG.kAug05

IKBKG.F12 (forward primer)
IKBKG.R10 (reverse primer)
Single exon skipping

Frame 0

VRGSSRPYWAVSS*FLPRRGRGGRAPRG*TPPS ERRGGV*AVTPCKVAGVLIITELPRACRAWRTQT SLRSGMRPYRGLTLLAGFPSSRGRGLCFYFRIP QL*HRKPEAW*GRATAKLGLSRSAGALPAFTVR RSHPSHV*RTLLTALALLDE*APLEEPTV*DGAA QWWPGSRSGRTGRRVSSGEASHAAPAFRTGRS* DPPALPGGESRAPRCHPAEQPDSAGALRGASAF PSQPEGGEGVPHVQVPGGQETGGETRPGEARSEE AEGAGSAGGGAPEEMPAADG*GQGLCESPGDVL ARGAAGEPESLGGCH*GMPGSGGSEEAGPVAGG LSPALPRIRQPHQEQRGGQ*AEAS

Frame 1

FAGQAGPIGQSLLDSSPGEGGAAERPEARRRRP RDEGACRR*RPAKWPACLSLPSFRGPAEPGGLR LLSGAGCGPTAASHFSPASRVLGGGACVFTSGSH SYDTGSRKRGREGRPRNWDFLGAPGPYQRSQSA APTLLTSDGLC*QPLPCWMNRHLWKSQLCEMVQ PSGGPAADQDVLGEESPLGKPAMLHLPSEQGAPE TLQRCLEENQELRDAIRQSNQILRERCEELLHF QASQREEKEFLMCKFQEARKLVERLGLEKLDLK RQKEQALREVEHLKRCQQQMAEDKASVKAQVTSL LGELQESQSRLEAATKECQALEGRRKLAQLQVA YHQLFQEYDNHIKSSVVGSERKRV

Frame 2

SRVKPALLGSLFLIPPPERAGRPSAPRLDAAVR ETRGRVGGDALQSGRRAYHYRASAGLQSLADSD FSPERDAALPRPHTSRRLPEFSGAGLVFLLPDP TAMTPEAGSVVGKGDRETGTFSERRGPTSVHSPP LPPFSRLTDSADSPCPVG*IGTSGRANCVRWCS PVVARQQIRTYWAKSLLWGSQPCCTCLQNRALL RPSSAAWRRIKSSEMPSGRATRFCGSAARSFCIS KPARGRRRSSSCASSRRPGNWWRDSAWRSSI*R GRRSRLCGRWST*RDASSRWLRTRPL*KPR*RP CSGSCRRARVAWRLPLRNARLWRVGGSWPSCRWP ITSSSKNTTTTSRAAWWAVSGSE*

Sequence IKBKG.gAug05

GTTCGCGGGTCAAGCCGGCCCTATTGGGCAGTCTCTTCTTGATTCCTCCCCCGGAGAGGGCGGGGCGGCCGAGCGCCCCGAGGCTAGACGCCGCCGTCCG AGAGACGAGGGGGCGTGTAGGCGGTGACGCCCTGCAAAGTGGCCGGCGTGCTTATCATTACCGAGCTTCCGCGGGCCTGCAGAGCCTGGCGGACTCAGAC TTCTCTCCGGAGCGGGATGCGGCCCTACCGCGGCCTCACACTTCTCGCCGGCTTCCCGAGTTCTCGGGGGCGGGGCTTGTGTTTTTACTTCCGGATCCCA CAGCTATGACACCGGAAGCCGGAAGCGTGGTAGGGAAGGGCGACCGCGAAACTGGGACTTTCTCGGAGCGCCGGGGCCCTACCAGCGTTCACAGTCCGCC GCTCCCACCCTTCTCACGTCTGACGGACTCTGCTGACAGCCCTTGCCCTGTTGGATGAATAGGCACCTCTGGAAGAGCCAACTGTGTGAGATGGTGCAGC CCAGTGGTGGCCCGGCAGCAGATCAGGACGTACTGGGCGAAGAGTCTCCTCTGGGGAAGCCAGCCATGCTGCACCTGCCTTCAGAACAGGGCGCTCCTGA GACCCTCCAGCGCTGCCTGGAGGAGAATCAAGAGCTCCGAGATGCCATCCGGCAGAGCAACCAGATTCTGCGGGAGCGCTGCGAGGAGCTTCTGCATTTC CAAGCCAGCCAGAGGGAGGAGAAGGAGTTCCTCATGTGCAAGTTCCAGGAGGCCAGGAAACTGGTGGAGAGACTCGGCCTGGAGAAGCTCGATCTGAAGA GGCAGAAGGAGCAGGCTCTGCGGGAGGTGGAGCACCTGAAGAGATGCCAGCAGCAGATGGCTGAGGACAAGGCCTCTGTGAAAGCCCAGGTGACGTCCTT GCTCGGGGAGCTGCAGGAGAGCCAGAGTCGCTTGGAGGCTGCCACTAAGGAATGCCAGGCTCTGGAGGGTCGGAGGAAGCTGGCCCAGTTGCAGGTGGCC TATCACCAGCTCTTCCAAGAATACGACAACCACATCAAGAGCAGCGTGGTGGGCAGTGAGCGGAAGCGAGCGGATATCTACAAGGCGGACTTCCAGGCTG AGAGGCAGGCCCGGGAGAAGCTGGCCGAGAAGAAGGAGCTCCTGCAGGAGCAGCTGGAGCAGCTGCAGAGGGAGTACAGCAAACTGAAGGCCAGCTGTCA GGAGTCGGCCAGGATCGAGGACATGAGGAAGCGGCATGTCGAGGTCTCCCAGGCCCCCTTGCCCCCCGCCCCTGCCTACCTCTCCTCTCCCCTGGCCCTG CCCAGCCAGAGGAGGAGCCCCCCCGAGGAGCCACCTGACTTCTGCTGTCCCAAGTGCCAGTATCAGGCCCCTGATATGGACACCCTGCAGATACATGTCA TGGAGTGCATTGAGTAGGGCCGGCCAGTGCAAGGCCACTGCCTGCCGAGGACGTGCCCGGGACCGTGCAGTCTGCGCTTTCCTCTCCCGCCTGCCTAGCC CAGGATGAAGGGCTGGGTGGCCACAACTGGGATGCCACCTGGAGCCCCACCCAGGAGCTGGCCGCGGCACCTTACGCTTCAGCTGTTGATCCGCTGGTCC CCTCTTTTGGGGTAGATGCGGCCCCGATCAGGCCTGACTCGCTGCTCTTTTTGTTCCCTTCTGTCTGCTCGAACCACTTGCCTCGGGCTAATCCCTCCCT CTTCCTCCACCCGGCACTGGGGAAGTCAAGAATGGGGCCTGGGGCTCTCAGGGAGAACTGCTTCCCCTGGCAGAGCTGGGTGGCAGCTCTTCCTCCCACC GGACACCGACCCGCCCGCTGCTGTGCCCTGGGAGTGCTGCCCTCTTACCATGCACACGGGTGCTCTCCTTTTGGGCTGCATGCTATTCCATTTTGCAGCC AGACCGATGTGTATTTAACCAGTCACTATTGATGGACATTTGGGTTGTTTCCCATCTTTTTGTTACCATAAATAATGGCATAGTAAAAATCCTTGTGCAT TA

IKBKG.F12 (forward primer)
IKBKG.R10 (reverse primer)
Single exon skipping

Frame 0

VRGSSRPYWAVSS*FLPRRGRGGRAPRG*TPPS ERRGGV*AVTPCKVAGVLIITELPRACRAWRTQT SLRSGMRPYRGLTLLAGFPSSRGRGLCFYFRIP QL*HRKPEAW*GRATAKLGLSRSAGALPAFTVR RSHPSHV*RTLLTALALLDE*APLEEPTV*DGAA QWWPGSRSGRTGRRVSSGEASHAAPAFRTGRS* DPPALPGGESRAPRCHPAEQPDSAGALRGASAF PSQPEGGEGVPHVQVPGGQETGGETRPGEARSEE AEGAGSAGGGAPEEMPAADG*GQGLCESPGDVL ARGAAGEPESLGGCH*GMPGSGGSEEAGPVAGG LSPALPRIRQPHQEQRGGQ*AEASGYLQGGLPG* EAGPGEAGREEGAPAGAAGAAAEGVQQTEGQLS GVGQDRGHEEAACRGLPGPLAPRPCLPLLSPGP AQPEEEPPRGAT*LLLSQVPVSGP*YGHPADTCH GVH*VGPASARPLPAEDVPGTVQSALSSPACLA QDEGLGGHNWDATWSPTQELAAAPYASAVDPLV PSFGVDAAPIRPDSLLFLFPSVCSNHLPRANPSL FLHPALGKSRMGPGALRENCFPWQSWVAALPPT GHRPARCCALGVLPSYHAHGCSPFGLHAIPFCS QTDVYLTSHY*WTFGLFPIFLLP*IMA**KSLCI

Frame 1

FAGQAGPIGQSLLDSSPGEGGAAERPEARRRRP RDEGACRR*RPAKWPACLSLPSFRGPAEPGGLR LLSGAGCGPTAASHFSPASRVLGGGACVFTSGSH SYDTGSRKRGREGRPRNWDFLGAPGPYQRSQSA APTLLTSDGLC*QPLPCWMNRHLWKSQLCEMVQ PSGGPAADQDVLGEESPLGKPAMLHLPSEQGAPE TLQRCLEENQELRDAIRQSNQILRERCEELLHF QASQREEKEFLMCKFQEARKLVERLGLEKLDLK RQKEQALREVEHLKRCQQQMAEDKASVKAQVTSL LGELQESQSRLEAATKECQALEGRRKLAQLQVA YHQLFQEYDNHIKSSVVGSERKRADIYKADFQA ERQAREKLAEKKELLQEQLEQLQREYSKLKASCQ ESARIEDMRKRHVEVSQAPLPPAPAYLSSPLAL PSQRRSPPEEPPDFCCPKCQYQAPDMDTLQIHV MECIE*GRPVQGHCLPRTCPGPCSLRFPLPPA*P RMKGWVATTGMPPGAPPRSWPRHLTLQLLIRWS PLLG*MRPRSGLTRCSFCSLLSARTTCLGLIPP SSSTRHWGSQEWGLGLSGRTASPGRAGWQLFLPP DTDPPAAVPWECCPLTMHTGALLLGCMLFHFAA RPMCI*PVTIDGHLGCFPSFCYHK*WHSKNPCA L

Frame 2

SRVKPALLGSLFLIPPPERAGRPSAPRLDAAVR ETRGRVGGDALQSGRRAYHYRASAGLQSLADSD FSPERDAALPRPHTSRRLPEFSGAGLVFLLPDP TAMTPEAGSVVGKGDRETGTFSERRGPTSVHSPP LPPFSRLTDSADSPCPVG*IGTSGRANCVRWCS PVVARQQIRTYWAKSLLWGSQPCCTCLQNRALL RPSSAAWRRIKSSEMPSGRATRFCGSAARSFCIS KPARGRRRSSSCASSRRPGNWWRDSAWRSSI*R GRRSRLCGRWST*RDASSRWLRTRPL*KPR*RP CSGSCRRARVAWRLPLRNARLWRVGGSWPSCRWP ITSSSKNTTTTSRAAWWAVSGSERISTRRTSRL RGRPGRSWPRRRSSCRSSWSSCRGSTAN*RPAV RSRPGSRT*GSGMSRSPRPPCPPPLPTSPLPWPC PARGGAPPRSHLTSAVPSASIRPLIWTPCRYMS WSALSRAGQCKATACRGRARDRAVCAFLSRLPS PG*RAGWPQLGCHLEPHPGAGRGTLRFSC*SAGP LFWGRCGPDQA*LAALFVPFCLLEPLASG*SLP LPPPGTGEVKNGAWGSQGELLPLAELGGSSSSH RTPTRPLLCPGSAALLPCTRVLSFWAACYSILQP DRCVFNQSLLMDIWVVSHLFVTINNGIVKILVH

Sequence IKBKG.fAug05

GTTCGCGGGTCAAGCCGGCCCTATTGGGCAGTCTCTTCTTGATTCCTCCCCCGGAGAGGGCGGGGCGGCCGAGCGCCCCGAGGCTAGACGCCGCCGTCCG AGAGACGAGGGGGCGTGTAGGCGGTGACGCCCTGCAAAGTGGCCGGCGTGCTTATCATTACCGAGCTTCCGCGGGCCTGCAGAGCCTGGCGGACTCAGAC TTCTCTCCGGAGCGGGATGCGGCCCTACCGCGGCCTCACACTTCTCGCCGGCTTCCCGAGTTCTCGGGGGCGGGGCTTGTGTTTTTACTTCCGGATCCCA CAGCTATGACACCGGAAGCCGGAAGCGTGGTAGGGAAGGGCGACCGCGAAACTGGGACTTTCTCGGAGCGCCGGGGCCCTACCAGCGTTCACAGTCCGCC GCTCCCACCCTTCTCACGTCTGACGGACTCTGCTGACAGCCCTTGCCCTGTTGGATGAATAGGCACCTCTGGAAGAGCCAACTGTGTGAGATGGTGCAGC CCAGTGGTGGCCCGGCAGCAGATCAGGACGTACTGGGCGAAGAGTCTCCTCTGGGGAAGCCAGCCATGCTGCACCTGCCTTCAGAACAGGGCGCTCCTGA GACCCTCCAGCGCTGCCTGGAGGAGAATCAAGAGCTCCGAGATGCCATCCGGCAGAGCAACCAGATTCTGCGGGAGCGCTGCGAGGAGCTTCTGCATTTC CAAGCCAGCCAGAGGGAGGAGAAGGAGTTCCTCATGTGCAAGTTCCAGGAGGCCAGGAAACTGGTGGAGAGACTCGGCCTGGAGAAGCTCGATCTGAAGA GGCAGAAGGAGCAGGCTCTGCGGGAGGTGGAGCACCTGAAGAGATGCCAGCAGCAGATGGCTGAGGACAAGGCCTCTGTGAAAGCCCAGGTGACGTCCTT GCTCGGGGAGCTGCAGGAGAGCCAGAGTCGCTTGGAGGCTGCCACTAAGGAATGCCAGGCTCTGGAGGGTCGGAGGAAGCTGGCCCAGTTGCAGGTGGCC TATCACCAGCTCTTCCAAGAATACGACAACCACATCAAGAGCAGCGTGGTGGGCAGTGAGCGGAAGCGAGGAATGCAGCTGGAAGATCTCAAACAGCAGC TCCAGCAGGCCGAGGAGGCCCTGGTGGCCAAACAGGAGGTGATCGATAAGCTGAAGGAGGAGGCCGAGCAGCACAAGATTGTGATGGAGACCGTTCCGGT GCTGAAGGCCCAGGCGGATATCTACAAGGCGGACTTCCAGGCTGAGAGGCAGGCCCGGGAGAAGCTGGCCGAGAAGAAGGAGCTCCTGCAGGAGCAGCTG GAGCAGCTGCAGAGGGAGTACAGCAAACTGAAGGCCAGCTGTCAGGAGTCGGCCAGGATCGAGGACATGAGGAAGCGGCATGTCGAGGTCTCCCAGGCCC CCTTGCCCCCCGCCCCTGCCTACCTCTCCTCTCCCCTGGCCCTGCCCAGCCAGAGGAGGAGCCCCCCCGAGGAGCCACCTGACTTCTGCTGTCCCAAGTG CCAGTATCAGGCCCCTGATATGGACACCCTGCAGATACATGTCATGGAGTGCATTGAGTAGGGCCGGCCAGTGCAAGGCCACTGCCTGCCGAGGACGTGC CCGGGACCGTGCAGTCTGCGCTTTCCTCTCCCGCCTGCCTAGCCCAGGATGAAGGGCTGGGTGGCCACAACTGGGATGCCACCTGGAGCCCCACCCAGGA GCTGGCCGCGGCACCTTACGCTTCAGCTGTTGATCCGCTGGTCCCCTCTTTTGGGGTAGATGCGGCCCCGATCAGGCCTGACTCGCTGCTCTTTTTGTTC CCTTCTGTCTGCTCGAACCACTTGCCTCGGGCTAATCCCTCCCTCTTCCTCCACCCGGCACTGGGGAAGTCAAGAATGGGGCCTGGGGCTCTCAGGGAGA ACTGCTTCCCCTGGCAGAGCTGGGTGGCAGCTCTTCCTCCCACCGGACACCGACCCGCCCGCTGCTGTGCCCTGGGAGTGCTGCCCTCTTACCATGCACA CGGGTGCTCTCCTTTTGGGCTGCATGCTATTCCATTTTGCAGCCAGACCGATGTGTATTTAACCAGTCACTATTGATGGACATTTGGGTTGTTTCCCATC TTTTTGTTACCATAAATAATGGCATAGTAAAAATCCTTGTGCATTA

IKBKG.F12 (forward primer)
IKBKG.R10 (reverse primer)
Single exon skipping

Frame 0

VRGSSRPYWAVSS*FLPRRGRGGRAPRG*TPPS ERRGGV*AVTPCKVAGVLIITELPRACRAWRTQT SLRSGMRPYRGLTLLAGFPSSRGRGLCFYFRIP QL*HRKPEAW*GRATAKLGLSRSAGALPAFTVR RSHPSHV*RTLLTALALLDE*APLEEPTV*DGAA QWWPGSRSGRTGRRVSSGEASHAAPAFRTGRS* DPPALPGGESRAPRCHPAEQPDSAGALRGASAF PSQPEGGEGVPHVQVPGGQETGGETRPGEARSEE AEGAGSAGGGAPEEMPAADG*GQGLCESPGDVL ARGAAGEPESLGGCH*GMPGSGGSEEAGPVAGG LSPALPRIRQPHQEQRGGQ*AEARNAAGRSQTAA PAGRGGPGGQTGGDR*AEGGGRAAQDCDGDRSG AEGPGGYLQGGLPG*EAGPGEAGREEGAPAGAA GAAAEGVQQTEGQLSGVGQDRGHEEAACRGLPGP LAPRPCLPLLSPGPAQPEEEPPRGAT*LLLSQV PVSGP*YGHPADTCHGVH*VGPASARPLPAEDV PGTVQSALSSPACLAQDEGLGGHNWDATWSPTQE LAAAPYASAVDPLVPSFGVDAAPIRPDSLLFLF PSVCSNHLPRANPSLFLHPALGKSRMGPGALRE NCFPWQSWVAALPPTGHRPARCCALGVLPSYHAH GCSPFGLHAIPFCSQTDVYLTSHY*WTFGLFPI FLLP*IMA**KSLCI

Frame 1

FAGQAGPIGQSLLDSSPGEGGAAERPEARRRRP RDEGACRR*RPAKWPACLSLPSFRGPAEPGGLR LLSGAGCGPTAASHFSPASRVLGGGACVFTSGSH SYDTGSRKRGREGRPRNWDFLGAPGPYQRSQSA APTLLTSDGLC*QPLPCWMNRHLWKSQLCEMVQ PSGGPAADQDVLGEESPLGKPAMLHLPSEQGAPE TLQRCLEENQELRDAIRQSNQILRERCEELLHF QASQREEKEFLMCKFQEARKLVERLGLEKLDLK RQKEQALREVEHLKRCQQQMAEDKASVKAQVTSL LGELQESQSRLEAATKECQALEGRRKLAQLQVA YHQLFQEYDNHIKSSVVGSERKRGMQLEDLKQQ LQQAEEALVAKQEVIDKLKEEAEQHKIVMETVPV LKAQADIYKADFQAERQAREKLAEKKELLQEQL EQLQREYSKLKASCQESARIEDMRKRHVEVSQA PLPPAPAYLSSPLALPSQRRSPPEEPPDFCCPKC QYQAPDMDTLQIHVMECIE*GRPVQGHCLPRTC PGPCSLRFPLPPA*PRMKGWVATTGMPPGAPPR SWPRHLTLQLLIRWSPLLG*MRPRSGLTRCSFCS LLSARTTCLGLIPPSSSTRHWGSQEWGLGLSGR TASPGRAGWQLFLPPDTDPPAAVPWECCPLTMH TGALLLGCMLFHFAARPMCI*PVTIDGHLGCFPS FCYHK*WHSKNPCAL

Frame 2

SRVKPALLGSLFLIPPPERAGRPSAPRLDAAVR ETRGRVGGDALQSGRRAYHYRASAGLQSLADSD FSPERDAALPRPHTSRRLPEFSGAGLVFLLPDP TAMTPEAGSVVGKGDRETGTFSERRGPTSVHSPP LPPFSRLTDSADSPCPVG*IGTSGRANCVRWCS PVVARQQIRTYWAKSLLWGSQPCCTCLQNRALL RPSSAAWRRIKSSEMPSGRATRFCGSAARSFCIS KPARGRRRSSSCASSRRPGNWWRDSAWRSSI*R GRRSRLCGRWST*RDASSRWLRTRPL*KPR*RP CSGSCRRARVAWRLPLRNARLWRVGGSWPSCRWP ITSSSKNTTTTSRAAWWAVSGSEECSWKISNSS SSRPRRPWWPNRR*SIS*RRRPSSTRL*WRPFR C*RPRRISTRRTSRLRGRPGRSWPRRRSSCRSSW SSCRGSTAN*RPAVRSRPGSRT*GSGMSRSPRP PCPPPLPTSPLPWPCPARGGAPPRSHLTSAVPS ASIRPLIWTPCRYMSWSALSRAGQCKATACRGRA RDRAVCAFLSRLPSPG*RAGWPQLGCHLEPHPG AGRGTLRFSC*SAGPLFWGRCGPDQA*LAALFV PFCLLEPLASG*SLPLPPPGTGEVKNGAWGSQGE LLPLAELGGSSSSHRTPTRPLLCPGSAALLPCT RVLSFWAACYSILQPDRCVFNQSLLMDIWVVSH LFVTINNGIVKILVH

Sequence IKBKG.hAug05

GTTCGCGGGTCAAGCCGGCCCTATTGGGCAGTCTCTTCTTGATTCCTCCCCCGGAGAGGGCGGGGCGGCCGAGCGCCCCGAGGCTAGACGCCGCCGTCCG AGAGACGAGGGGGCGTGTAGGCGGTGACGCCCTGCAAAGTGGCCGGCGTGCTTATCATTACCGAGCTTCCGCGGGCCTGCAGAGCCTGGCGGACTCAGAC TTCTCTCCGGAGCGGGATGCGGCCCTACCGCGGCCTCACACTTCTCGCCGGCTTCCCGAGTTCTCGGGGGCGGGGCTTGTGTTTTTACTTCCGGATCCCA CAGCTATGACACCGGAAGCCGGAAGCGTGGTAGGGAAGGGCGACCGCGAAACTGGGACTTTCTCGGAGCGCCGGGGCCCTACCAGCGTTCACAGTCCGCC GCTCCCACCCTTCTCACGTCTGACGGACTCTGCTGACAGCCCTTGCCCTGTTGGATGAATAGGCACCTCTGGAAGAGCCAACTGTGTGAGATGGTGCAGC CCAGTGGTGGCCCGGCAGCAGATCAGGACGTACTGGGCGAAGAGTCTCCTCTGGGGAAGCCAGCCATGCTGCACCTGCCTTCAGAACAGGGCGCTCCTGA GACCCTCCAGCGCTGCCTGGAGGAGAATCAAGAGCTCCGAGATGCCATCCGGCAGAGCAACCAGATTCTGCGGGAGCGCTGCGAGGAGCTTCTGCATTTC CAAGCCAGCCAGAGGGAGGAGAAGGAGTTCCTCATGTGCAAGTTCCAGGAGGCCAGGAAACTGGTGGAGAGACTCGGCCTGGAGAAGCTCGATCTGAAGA GGCAGAAGGAGCAGGCTCTGCGGGAGGTGGAGCACCTGAAGAGATGCCAGCAGCAGATGGCTGAGGACAAGGCCTCTGTGAAAGCCCAGGTGACGTCCTT GCTCGGGGAGCTGCAGGAGAGCCAGAGTCGCTTGGAGGCTGCCACTAAGGAATGCCAGGCTCTGGAGGGTCGGGCCCGGGCGGCCAGCGAGCAGGCGCGG CAGCTGGAGAGTGAGCGCGAGGCGCTGCAGCAGCAGCACAGCGTGCAGGTGGACCAGCTGCGCATGCAGGGCCAGAGCGTGGAGGCCGCGCTCCGCATGG AGCGCCAGGCCGCCTCGGAGGAGAAGAGGAAGCTGGCCCAGTTGCAGGTGGCCTATCACCAGCTCTTCCAAGAATACGACAACCACATCAAGAGCAGCGT GGTGGGCAGTGAGCGGAAGCGAGTGAGGAATGCAGCTGGAAGATCTCAAACAGCAGCTCCAGCAGGCCGAGGAGGCCCTGGTGGCCAAACAGGAGGTGAT CGATAAGCTGAAGGAGGAGGCCGAGCAGCACAAGATTGTGATGGAGACCGTTCCGGTGCTGAAGGCCCAGGCGGATATCTACAAGGCGGACTTCCAGGCT GAGAGGCAGGCCCGGGAGAAGCTGGCCGAGAAGAAGGAGCTCCTGCAGGAGCAGCTGGAGCAGCTGCAGAGGGAGTACAGCAAACTGAAGGCCAGCTGTC AGGAGTCGGCCAGGATCGAGGACATGAGGAAGCGGCATGTCGAGGTCTCCCAGGCCCCCTTGCCCCCCGCCCCTGCCTACCTCTCCTCTCCCCTGGCCCT GCCCAGCCAGAGGAGGAGCCCCCCCGAGGAGCCACCTGACTTCTGCTGTCCCAAGTGCCAGTATCAGGCCCCTGATATGGACACCCTGCAGATACATGTC ATGGAGTGCATTGAGTAGGGCCGGCCAGTGCAAGGCCACTGCCTGCCGAGGACGTGCCCGGGACCGTGCAGTCTGCGCTTTCCTCTCCCGCCTGCCTAGC CCAGGATGAAGGGCTGGGTGGCCACAACTGGGATGCCACCTGGAGCCCCACCCAGGAGCTGGCCGCGGCACCTTACGCTTCAGCTGTTGATCCGCTGGTC CCCTCTTTTGGGGTAGATGCGGCCCCGATCAGGCCTGACTCGCTGCTCTTTTTGTTCCCTTCTGTCTGCTCGAACCACTTGCCTCGGGCTAATCCCTCCC TCTTCCTCCACCCGGCACTGGGGAAGTCAAGAATGGGGCCTGGGGCTCTCAGGGAGAACTGCTTCCCCTGGCAGAGCTGGGTGGCAGCTCTTCCTCCCAC CGGACACCGACCCGCCCGCTGCTGTGCCCTGGGAGTGCTGCCCTCTTACCATGCACACGGGTGCTCTCCTTTTGGGCTGCATGCTATTCCATTTTGCAGC CAGACCGATGTGTATTTAACCAGTCACTATTGATGGACATTTGGGTTGTTTCCCATCTTTTTGTTACCATAAATAATGGCATAGTAAAAATCCTTGTGCA TTA

IKBKG.F12 (forward primer)
IKBKG.R10 (reverse primer)
Single exon skipping

Frame 0

VRGSSRPYWAVSS*FLPRRGRGGRAPRG*TPPS ERRGGV*AVTPCKVAGVLIITELPRACRAWRTQT SLRSGMRPYRGLTLLAGFPSSRGRGLCFYFRIP QL*HRKPEAW*GRATAKLGLSRSAGALPAFTVR RSHPSHV*RTLLTALALLDE*APLEEPTV*DGAA QWWPGSRSGRTGRRVSSGEASHAAPAFRTGRS* DPPALPGGESRAPRCHPAEQPDSAGALRGASAF PSQPEGGEGVPHVQVPGGQETGGETRPGEARSEE AEGAGSAGGGAPEEMPAADG*GQGLCESPGDVL ARGAAGEPESLGGCH*GMPGSGGSGPGGQRAGA AAGE*ARGAAAAAQRAGGPAAHAGPERGGRAPHG APGRLGGEEEAGPVAGGLSPALPRIRQPHQEQR GGQ*AEASEECSWKISNSSSSRPRRPWWPNRR* SIS*RRRPSSTRL*WRPFRC*RPRRISTRRTSRL RGRPGRSWPRRRSSCRSSWSSCRGSTAN*RPAV RSRPGSRT*GSGMSRSPRPPCPPPLPTSPLPWP CPARGGAPPRSHLTSAVPSASIRPLIWTPCRYMS WSALSRAGQCKATACRGRARDRAVCAFLSRLPS PG*RAGWPQLGCHLEPHPGAGRGTLRFSC*SAG PLFWGRCGPDQA*LAALFVPFCLLEPLASG*SLP LPPPGTGEVKNGAWGSQGELLPLAELGGSSSSH RTPTRPLLCPGSAALLPCTRVLSFWAACYSILQ PDRCVFNQSLLMDIWVVSHLFVTINNGIVKILVH

Frame 1

FAGQAGPIGQSLLDSSPGEGGAAERPEARRRRP RDEGACRR*RPAKWPACLSLPSFRGPAEPGGLR LLSGAGCGPTAASHFSPASRVLGGGACVFTSGSH SYDTGSRKRGREGRPRNWDFLGAPGPYQRSQSA APTLLTSDGLC*QPLPCWMNRHLWKSQLCEMVQ PSGGPAADQDVLGEESPLGKPAMLHLPSEQGAPE TLQRCLEENQELRDAIRQSNQILRERCEELLHF QASQREEKEFLMCKFQEARKLVERLGLEKLDLK RQKEQALREVEHLKRCQQQMAEDKASVKAQVTSL LGELQESQSRLEAATKECQALEGRARAASEQAR QLESEREALQQQHSVQVDQLRMQGQSVEAALRM ERQAASEEKRKLAQLQVAYHQLFQEYDNHIKSSV VGSERKRVRNAAGRSQTAAPAGRGGPGGQTGGD R*AEGGGRAAQDCDGDRSGAEGPGGYLQGGLPG *EAGPGEAGREEGAPAGAAGAAAEGVQQTEGQLS GVGQDRGHEEAACRGLPGPLAPRPCLPLLSPGP AQPEEEPPRGAT*LLLSQVPVSGP*YGHPADTC HGVH*VGPASARPLPAEDVPGTVQSALSSPACLA QDEGLGGHNWDATWSPTQELAAAPYASAVDPLV PSFGVDAAPIRPDSLLFLFPSVCSNHLPRANPS LFLHPALGKSRMGPGALRENCFPWQSWVAALPPT GHRPARCCALGVLPSYHAHGCSPFGLHAIPFCS QTDVYLTSHY*WTFGLFPIFLLP*IMA**KSLC I

Frame 2

SRVKPALLGSLFLIPPPERAGRPSAPRLDAAVR ETRGRVGGDALQSGRRAYHYRASAGLQSLADSD FSPERDAALPRPHTSRRLPEFSGAGLVFLLPDP TAMTPEAGSVVGKGDRETGTFSERRGPTSVHSPP LPPFSRLTDSADSPCPVG*IGTSGRANCVRWCS PVVARQQIRTYWAKSLLWGSQPCCTCLQNRALL RPSSAAWRRIKSSEMPSGRATRFCGSAARSFCIS KPARGRRRSSSCASSRRPGNWWRDSAWRSSI*R GRRSRLCGRWST*RDASSRWLRTRPL*KPR*RP CSGSCRRARVAWRLPLRNARLWRVGPGRPASRRG SWRVSARRCSSSTACRWTSCACRARAWRPRSAW SARPPRRRRGSWPSCRWPITSSSKNTTTTSRAA WWAVSGSE*GMQLEDLKQQLQQAEEALVAKQEVI DKLKEEAEQHKIVMETVPVLKAQADIYKADFQA ERQAREKLAEKKELLQEQLEQLQREYSKLKASC QESARIEDMRKRHVEVSQAPLPPAPAYLSSPLAL PSQRRSPPEEPPDFCCPKCQYQAPDMDTLQIHV MECIE*GRPVQGHCLPRTCPGPCSLRFPLPPA* PRMKGWVATTGMPPGAPPRSWPRHLTLQLLIRWS PLLG*MRPRSGLTRCSFCSLLSARTTCLGLIPP SSSTRHWGSQEWGLGLSGRTASPGRAGWQLFLP PDTDPPAAVPWECCPLTMHTGALLLGCMLFHFAA RPMCI*PVTIDGHLGCFPSFCYHK*WHSKNPCA L

Sequence IKBKG.eAug05

AGCCCGTTCCTGCTCCGCGCTTCTGGAGCACTGGCCAAGGCGGGCCGATTCAGGACCCAGGTTACTTGGGCGGCGAGCTGGACTGTTTCTACTCCTCCCT CCTCCTCCACTGCGGGGTCTGACCCTACTCCTTGTGTGAGGACTCCTCTAGTTCAGAGACATATTCTGTTCACCAAACTTGACTGCGCTCTATCGAGGTC GTTAAATTCTTCGGAAATGCCTCACATATAGTTTGGCAGCTAGCCCTTGCCCTGTTGGATGAATAGGCACCTCTGGAAGAGCCAACTGTGTGAGATGGTG CAGCCCAGTGGTGGCCCGGCAGCAGATCAGGACGTACTGGGCGAAGAGTCTCCTCTGGGGAAGCCAGCCATGCTGCACCTGCCTTCAGAACAGGGCGCTC CTGAGACCCTCCAGCGCTGCCTGGAGGAGAATCAAGAGCTCCGAGATGCCATCCGGCAGAGCAACCAGATTCTGCGGGAGCGCTGCGAGGAGCTTCTGCA TTTCCAAGCCAGCCAGAGGGAGGAGAAGGAGTTCCTCATGTGCAAGTTCCAGGAGGCCAGGAAACTGGTGGAGAGACTCGGCCTGGAGAAGCTCGATCTG AAGAGGCAGAAGGAGCAGGCTCTGCGGGAGGTGGAGCACCTGAAGAGATGCCAGCAGCAGATGGCTGAGGACAAGGCCTCTGTGAAAGCCCAGGTGACGT CCTTGCTCGGGGAGCTGCAGGAGAGCCAGAGTCGCTTGGAGGCTGCCACTAAGGAATGCCAGGCTCTGGAGGGTCGGGCCCGGGCGGCCAGCGAGCAGGC GCGGCAGCTGGAGAGTGAGCGCGAGGCGCTGCAGCAGCAGCACAGCGTGCAGGTGGACCAGCTGCGCATGCAGGGCCAGAGCGTGGAGGCCGCGCTCCGC ATGGAGCGCCAGGCCGCCTCGGAGGAGAAGAGGAAGCTGGCCCAGTTGCAGGTGGCCTATCACCAGCTCTTCCAAGAATACGACAACCACATCAAGAGCA GCGTGGTGGGCAGTGAGCGGAAGCGAGGAATGCAGCTGGAAGATCTCAAACAGCAGCTCCAGCAGGCCGAGGAGGCCCTGGTGGCCAAACAGGAGGTGAT CGATAAGCTGAAGGAGGAGGCCGAGCAGCACAAGATTGTGATGGAGACCGTTCCGGTGCTGAAGGCCCAGGCGGATATCTACAAGGCGGACTTCCAGGCT GAGAGGCAGGCCCGGGAGAAGCTGGCCGAGAAGAAGGAGCTCCTGCAGGAGCAGCTGGAGCAGCTGCAGAGGGAGTACAGCAAACTGAAGGCCAGCTGTC AGGAGTCGGCCAGGATCGAGGACATGAGGAAGCGGCATGTCGAGGTCTCCCAGGCCCCCTTGCCCCCCGCCCCTGCCTACCTCTCCTCTCCCCTGGCCCT GCCCAGCCAGAGGAGGAGCCCCCCCGAGGAGCCACCTGACTTCTGCTGTCCCAAGTGCCAGTATCAGGCCCCTGATATGGACACCCTGCAGATACATGTC ATGGAGTGCATTGAGTAGGGCCGGCCAGTGCAAGGCCACTGCCTGCCGAGGACGTGCCCGGGACCGTGCAGTCTGCGCTTTCCTCTCCCGCCTGCCTAGC CCAGGATGAAGGGCTGGGTGGCCACAACTGGGATGCCACCTGGAGCCCCACCCAGGAGCTGGCCGCGGCACCTTACGCTTCAGCTGTTGATCCGCTGGTC CCCTCTTTTGGGGTAGATGCGGCCCCGATCAGGCCTGACTCGCTGCTCTTTTTGTTCCCTTCTGTCTGCTCGAACCACTTGCCTCGGGCTAATCCCTCCC TCTTCCTCCACCCGGCACTGGGGAAGTCAAGAATGGGGCCTGGGGCTCTCAGGGAGAACTGCTTCCCCTGGCAGAGCTGGGTGGCAGCTCTTCCTCCCAC CGGACACCGACCCGCCCGCTGCTGTGCCCTGGGAGTGCTGCCCTCTTACCATGCACACGGGTGCTCTCCTTTTGGGCTGCATGCTATTCCATTTTGCAGC CAGACCGATGTGTATTTAACCAGTCACTATTGATGGACATTTGGGTTGTTTCCCATCTTTTTGTTACCATAAATAATGGCATAGTAAAAATCCTTGTGCA TTA

IKBKG.F12 (forward primer)
IKBKG.R10 (reverse primer)
Single exon skipping

Frame 0

SPFLLRASGALAKAGRFRTQVTWAASWTVSTPP SSSTAGSDPTPCVRTPLVQRHILFTKLDCALSRS LNSSEMPHI*FGS*PLPCWMNRHLWKSQLCEMV QPSGGPAADQDVLGEESPLGKPAMLHLPSEQGA PETLQRCLEENQELRDAIRQSNQILRERCEELLH FQASQREEKEFLMCKFQEARKLVERLGLEKLDL KRQKEQALREVEHLKRCQQQMAEDKASVKAQVT SLLGELQESQSRLEAATKECQALEGRARAASEQA RQLESEREALQQQHSVQVDQLRMQGQSVEAALR MERQAASEEKRKLAQLQVAYHQLFQEYDNHIKS SVVGSERKRGMQLEDLKQQLQQAEEALVAKQEVI DKLKEEAEQHKIVMETVPVLKAQADIYKADFQA ERQAREKLAEKKELLQEQLEQLQREYSKLKASC QESARIEDMRKRHVEVSQAPLPPAPAYLSSPLAL PSQRRSPPEEPPDFCCPKCQYQAPDMDTLQIHV MECIE*GRPVQGHCLPRTCPGPCSLRFPLPPA* PRMKGWVATTGMPPGAPPRSWPRHLTLQLLIRWS PLLG*MRPRSGLTRCSFCSLLSARTTCLGLIPP SSSTRHWGSQEWGLGLSGRTASPGRAGWQLFLP PDTDPPAAVPWECCPLTMHTGALLLGCMLFHFAA RPMCI*PVTIDGHLGCFPSFCYHK*WHSKNPCA L

Frame 1

ARSCSALLEHWPRRADSGPRLLGRRAGLFLLLP PPPLRGLTLLLV*GLL*FRDIFCSPNLTALYRG R*ILRKCLTYSLAASPCPVG*IGTSGRANCVRWC SPVVARQQIRTYWAKSLLWGSQPCCTCLQNRAL LRPSSAAWRRIKSSEMPSGRATRFCGSAARSFC ISKPARGRRRSSSCASSRRPGNWWRDSAWRSSI* RGRRSRLCGRWST*RDASSRWLRTRPL*KPR*R PCSGSCRRARVAWRLPLRNARLWRVGPGRPASR RGSWRVSARRCSSSTACRWTSCACRARAWRPRSA WSARPPRRRRGSWPSCRWPITSSSKNTTTTSRA AWWAVSGSEECSWKISNSSSSRPRRPWWPNRR* SIS*RRRPSSTRL*WRPFRC*RPRRISTRRTSRL RGRPGRSWPRRRSSCRSSWSSCRGSTAN*RPAV RSRPGSRT*GSGMSRSPRPPCPPPLPTSPLPWP CPARGGAPPRSHLTSAVPSASIRPLIWTPCRYMS WSALSRAGQCKATACRGRARDRAVCAFLSRLPS PG*RAGWPQLGCHLEPHPGAGRGTLRFSC*SAG PLFWGRCGPDQA*LAALFVPFCLLEPLASG*SLP LPPPGTGEVKNGAWGSQGELLPLAELGGSSSSH RTPTRPLLCPGSAALLPCTRVLSFWAACYSILQ PDRCVFNQSLLMDIWVVSHLFVTINNGIVKILVH

Frame 2

PVPAPRFWSTGQGGPIQDPGYLGGELDCFYSSL LLHCGV*PYSLCEDSSSSETYSVHQT*LRSIEV VKFFGNASHIVWQLALALLDE*APLEEPTV*DG AAQWWPGSRSGRTGRRVSSGEASHAAPAFRTGRS *DPPALPGGESRAPRCHPAEQPDSAGALRGASA FPSQPEGGEGVPHVQVPGGQETGGETRPGEARS EEAEGAGSAGGGAPEEMPAADG*GQGLCESPGDV LARGAAGEPESLGGCH*GMPGSGGSGPGGQRAG AAAGE*ARGAAAAAQRAGGPAAHAGPERGGRAP HGAPGRLGGEEEAGPVAGGLSPALPRIRQPHQEQ RGGQ*AEARNAAGRSQTAAPAGRGGPGGQTGGD R*AEGGGRAAQDCDGDRSGAEGPGGYLQGGLPG *EAGPGEAGREEGAPAGAAGAAAEGVQQTEGQLS GVGQDRGHEEAACRGLPGPLAPRPCLPLLSPGP AQPEEEPPRGAT*LLLSQVPVSGP*YGHPADTC HGVH*VGPASARPLPAEDVPGTVQSALSSPACLA QDEGLGGHNWDATWSPTQELAAAPYASAVDPLV PSFGVDAAPIRPDSLLFLFPSVCSNHLPRANPS LFLHPALGKSRMGPGALRENCFPWQSWVAALPPT GHRPARCCALGVLPSYHAHGCSPFGLHAIPFCS QTDVYLTSHY*WTFGLFPIFLLP*IMA**KSLC I

Sequence IKBKG.dAug05

GTTCGCGGGTCAAGCCGGCCCTATTGGGCAGTCTCTTCTTGATTCCTCCCCCGGAGAGGGCGGGGCGGCCGAGCGCCCCGAGGCTAGACGCCGCCGTCCG AGAGACGAGGGGGCGTGTAGGCGGTGACGCCCTGCAAAGTGGCCGGCGTGCTTATCATTACCGAGCTTCCGCGGGCCTGCAGAGCCTGGCGGACTCAGAC TTCTCTCCGGAGCGGGATGCGGCCCTACCGCGGCCTCACACTTCTCGCCGGCTTCCCGAGTTCTCGGGGGCGGGGCTTGTGTTTTTACTTCCGGATCCCA CAGCTATGACACCGGAAGCCGGAAGCGTGGTAGGGAAGGGCGACCGCGAAACTGGGACTTTCTCGGAGCGCCGGGGCCCTACCAGCGTTCACAGTCCGCC GCTCCCACCCTTCTCACGTCTGACGGACTCTGCTGACAGCCCTTGCCCTGTTGGATGAATAGGCACCTCTGGAAGAGCCAACTGTGTGAGATGGTGCAGC CCAGTGGTGGCCCGGCAGCAGATCAGGACGTACTGGGCGAAGAGTCTCCTCTGGGGAAGCCAGCCATGCTGCACCTGCCTTCAGAACAGGGCGCTCCTGA GACCCTCCAGCGCTGCCTGGAGGAGAATCAAGAGCTCCGAGATGCCATCCGGCAGAGCAACCAGATTCTGCGGGAGCGCTGCGAGGAGCTTCTGCATTTC CAAGCCAGCCAGAGGGAGGAGAAGGAGTTCCTCATGTGCAAGTTCCAGGAGGCCAGGAAACTGGTGGAGAGACTCGGCCTGGAGAAGCTCGATCTGAAGA GGCAGAAGGAGCAGGCTCTGCGGGAGGTGGAGCACCTGAAGAGATGCCAGCAGATGGCTGAGGACAAGGCCTCTGTGAAAGCCCAGGTGACGTCCTTGCT CGGGGAGCTGCAGGAGAGCCAGAGTCGCTTGGAGGCTGCCACTAAGGAATGCCAGGCTCTGGAGGGTCGGGCCCGGGCGGCCAGCGAGCAGGCGCGGCAG CTGGAGAGTGAGCGCGAGGCGCTGCAGCAGCAGCACAGCGTGCAGGTGGACCAGCTGCGCATGCAGGGCCAGAGCGTGGAGGCCGCGCTCCGCATGGAGC GCCAGGCCGCCTCGGAGGAGAAGAGGAAGCTGGCCCAGTTGCAGGTGGCCTATCACCAGCTCTTCCAAGAATACGACAACCACATCAAGAGCAGCGTGGT GGGCAGTGAGCGGAAGCGAGGAATGCAGCTGGAAGATCTCAAACAGCAGCTCCAGCAGGCCGAGGAGGCCCTGGTGGCCAAACAGGAGGTGATCGATAAG CTGAAGGAGGAGGCCGAGCAGCACAAGATTGTGATGGAGACCGTTCCGGTGCTGAAGGCCCAGGCGGATATCTACAAGGCGGACTTCCAGGCTGAGAGGC AGGCCCGGGAGAAGCTGGCCGAGAAGAAGGAGCTCCTGCAGGAGCAGCTGGAGCAGCTGCAGAGGGAGTACAGCAAACTGAAGGCCAGCTGTCAGGAGTC GGCCAGGATCGAGGACATGAGGAAGCGGCATGTCGAGGTCTCCCAGGCCCCCTTGCCCCCCGCCCCTGCCTACCTCTCCTCTCCCCTGGCCCTGCCCAGC CAGAGGAGGAGCCCCCCCGAGGAGCCACCTGACTTCTGCTGTCCCAAGTGCCAGTATCAGGCCCCTGATATGGACACCCTGCAGATACATGTCATGGAGT GCATTGAGTAGGGCCGGCCAGTGCAAGGCCACTGCCTGCCGAGGACGTGCCCGGGACCGTGCAGTCTGCGCTTTCCTCTCCCGCCTGCCTAGCCCAGGAT GAAGGGCTGGGTGGCCACAACTGGGATGCCACCTGGAGCCCCACCCAGGAGCTGGCCGCGGCACCTTACGCTTCAGCTGTTGATCCGCTGGTCCCCTCTT TTGGGGTAGATGCGGCCCCGATCAGGCCTGACTCGCTGCTCTTTTTGTTCCCTTCTGTCTGCTCGAACCACTTGCCTCGGGCTAATCCCTCCCTCTTCCT CCACCCGGCACTGGGGAAGTCAAGAATGGGGCCTGGGGCTCTCAGGGAGAACTGCTTCCCCTGGCAGAGCTGGGTGGCAGCTCTTCCTCCCACCGGACAC CGACCCGCCCGCTGCTGTGCCCTGGGAGTGCTGCCCTCTTACCATGCACACGGGTGCTCTCCTTTTGGGCTGCATGCTATTCCATTTTGCAGCCAGACCG ATGTGTATTTAACCAGTCACTATTGATGGACATTTGGGTTGTTTCCCATCTTTTTGTTACCATAAATAATGGCATAGTAAAAATCCTTGTGCATTA

IKBKG.F12 (forward primer)
IKBKG.R10 (reverse primer)
Single exon skipping

Frame 0

VRGSSRPYWAVSS*FLPRRGRGGRAPRG*TPPS ERRGGV*AVTPCKVAGVLIITELPRACRAWRTQT SLRSGMRPYRGLTLLAGFPSSRGRGLCFYFRIP QL*HRKPEAW*GRATAKLGLSRSAGALPAFTVR RSHPSHV*RTLLTALALLDE*APLEEPTV*DGAA QWWPGSRSGRTGRRVSSGEASHAAPAFRTGRS* DPPALPGGESRAPRCHPAEQPDSAGALRGASAF PSQPEGGEGVPHVQVPGGQETGGETRPGEARSEE AEGAGSAGGGAPEEMPADG*GQGLCESPGDVLA RGAAGEPESLGGCH*GMPGSGGSGPGGQRAGAA AGE*ARGAAAAAQRAGGPAAHAGPERGGRAPHGA PGRLGGEEEAGPVAGGLSPALPRIRQPHQEQRG GQ*AEARNAAGRSQTAAPAGRGGPGGQTGGDR* AEGGGRAAQDCDGDRSGAEGPGGYLQGGLPG*EA GPGEAGREEGAPAGAAGAAAEGVQQTEGQLSGV GQDRGHEEAACRGLPGPLAPRPCLPLLSPGPAQ PEEEPPRGAT*LLLSQVPVSGP*YGHPADTCHGV H*VGPASARPLPAEDVPGTVQSALSSPACLAQD EGLGGHNWDATWSPTQELAAAPYASAVDPLVPS FGVDAAPIRPDSLLFLFPSVCSNHLPRANPSLFL HPALGKSRMGPGALRENCFPWQSWVAALPPTGH RPARCCALGVLPSYHAHGCSPFGLHAIPFCSQT DVYLTSHY*WTFGLFPIFLLP*IMA**KSLCI

Frame 1

FAGQAGPIGQSLLDSSPGEGGAAERPEARRRRP RDEGACRR*RPAKWPACLSLPSFRGPAEPGGLR LLSGAGCGPTAASHFSPASRVLGGGACVFTSGSH SYDTGSRKRGREGRPRNWDFLGAPGPYQRSQSA APTLLTSDGLC*QPLPCWMNRHLWKSQLCEMVQ PSGGPAADQDVLGEESPLGKPAMLHLPSEQGAPE TLQRCLEENQELRDAIRQSNQILRERCEELLHF QASQREEKEFLMCKFQEARKLVERLGLEKLDLK RQKEQALREVEHLKRCQQMAEDKASVKAQVTSLL GELQESQSRLEAATKECQALEGRARAASEQARQ LESEREALQQQHSVQVDQLRMQGQSVEAALRME RQAASEEKRKLAQLQVAYHQLFQEYDNHIKSSVV GSERKRGMQLEDLKQQLQQAEEALVAKQEVIDK LKEEAEQHKIVMETVPVLKAQADIYKADFQAER QAREKLAEKKELLQEQLEQLQREYSKLKASCQES ARIEDMRKRHVEVSQAPLPPAPAYLSSPLALPS QRRSPPEEPPDFCCPKCQYQAPDMDTLQIHVME CIE*GRPVQGHCLPRTCPGPCSLRFPLPPA*PRM KGWVATTGMPPGAPPRSWPRHLTLQLLIRWSPL LG*MRPRSGLTRCSFCSLLSARTTCLGLIPPSS STRHWGSQEWGLGLSGRTASPGRAGWQLFLPPDT DPPAAVPWECCPLTMHTGALLLGCMLFHFAARP MCI*PVTIDGHLGCFPSFCYHK*WHSKNPCAL

Frame 2

SRVKPALLGSLFLIPPPERAGRPSAPRLDAAVR ETRGRVGGDALQSGRRAYHYRASAGLQSLADSD FSPERDAALPRPHTSRRLPEFSGAGLVFLLPDP TAMTPEAGSVVGKGDRETGTFSERRGPTSVHSPP LPPFSRLTDSADSPCPVG*IGTSGRANCVRWCS PVVARQQIRTYWAKSLLWGSQPCCTCLQNRALL RPSSAAWRRIKSSEMPSGRATRFCGSAARSFCIS KPARGRRRSSSCASSRRPGNWWRDSAWRSSI*R GRRSRLCGRWST*RDASRWLRTRPL*KPR*RPC SGSCRRARVAWRLPLRNARLWRVGPGRPASRRGS WRVSARRCSSSTACRWTSCACRARAWRPRSAWS ARPPRRRRGSWPSCRWPITSSSKNTTTTSRAAW WAVSGSEECSWKISNSSSSRPRRPWWPNRR*SIS *RRRPSSTRL*WRPFRC*RPRRISTRRTSRLRG RPGRSWPRRRSSCRSSWSSCRGSTAN*RPAVRS RPGSRT*GSGMSRSPRPPCPPPLPTSPLPWPCPA RGGAPPRSHLTSAVPSASIRPLIWTPCRYMSWS ALSRAGQCKATACRGRARDRAVCAFLSRLPSPG *RAGWPQLGCHLEPHPGAGRGTLRFSC*SAGPLF WGRCGPDQA*LAALFVPFCLLEPLASG*SLPLP PPGTGEVKNGAWGSQGELLPLAELGGSSSSHRT PTRPLLCPGSAALLPCTRVLSFWAACYSILQPDR CVFNQSLLMDIWVVSHLFVTINNGIVKILVH

Sequence IKBKG.cAug05

GTTCGCGGGTCAAGCCGGCCCTATTGGGCAGTCTCTTCTTGATTCCTCCCCCGGAGAGGGCGGGGCGGCCGAGCGCCCCGAGGCTAGACGCCGCCGTCCG AGAGACGAGGGGGCGTGTAGGCGGTGACGCCCTGCAAAGTGGCCGGCGTGCTTATCATTACCGAGCTTCCGCGGGCCTGCAGAGCCTGGCGGACTCAGAC TTCTCTCCGGAGCGGGATGCGGCCCTACCGCGGCCTCACACTTCTCGCCGGCTTCCCGAGTTCTCGGGGGCGGGGCTTGTGTTTTTACTTCCGGATCCCA CAGCTATGACACCGGAAGCCGGAAGCGTGGTAGGGAAGGGCGACCGCGAAACTGGGACTTTCTCGGAGCGCCGGGGCCCTACCAGCGTTCACAGTCCGCC GCTCCCACCCTTCTCACGTCTGACGGACTCTGCTGACAGCCCTTGCCCTGTTGGATGAATAGGCACCTCTGGAAGAGCCAACTGTGTGAGATGGTGCAGC CCAGTGGTGGCCCGGCAGCAGATCAGGACGTACTGGGCGAAGAGTCTCCTCTGGGGAAGCCAGCCATGCTGCACCTGCCTTCAGAACAGGGCGCTCCTGA GACCCTCCAGCGCTGCCTGGAGGAGAATCAAGAGCTCCGAGATGCCATCCGGCAGAGCAACCAGATTCTGCGGGAGCGCTGCGAGGAGCTTCTGCATTTC CAAGCCAGCCAGAGGGAGGAGAAGGAGTTCCTCATGTGCAAGTTCCAGGAGGCCAGGAAACTGGTGGAGAGACTCGGCCTGGAGAAGCTCGATCTGAAGA GGCAGAAGGAGCAGGCTCTGCGGGAGGTGGAGCACCTGAAGAGATGCCAGCAGCAGATGGCTGAGGACAAGGCCTCTGTGAAAGCCCAGGTGACGTCCTT GCTCGGGGAGCTGCAGGAGAGCCAGAGTCGCTTGGAGGCTGCCACTAAGGAATGCCAGGCTCTGGAGGGTCGGGCCCGGGCGGCCAGCGAGCAGGCGCGG CAGCTGGAGAGTGAGCGCGAGGCGCTGCAGCAGCAGCACAGCGTGCAGGTGGACCAGCTGCGCATGCAGGGCCAGAGCGTGGAGGCCGCGCTCCGCATGG AGCGCCAGGCCGCCTCGGAGGAGAAGAGGAAGCTGGCCCAGTTGCAGGTGGCCTATCACCAGCTCTTCCAAGAATACGACAACCACATCAAGAGCAGCGT GGTGGGCAGTGAGCGGAAGCGAGGAATGCAGCTGGAAGATCTCAAACAGCAGCTCCAGCAGGCCGAGGAGGCCCTGGTGGCCAAACAGGAGGTGATCGAT AAGCTGAAGGAGGAGGCCGAGCAGCACAAGATTGTGATGGAGACCGTTCCGGTGCTGAAGGCCCAGGCGGATATCTACAAGGCGGACTTCCAGGCTGAGA GGCAGGCCCGGGAGAAGCTGGCCGAGAAGAAGGAGCTCCTGCAGGAGCAGCTGGAGCAGCTGCAGAGGGAGTACAGCAAACTGAAGGCCAGCTGTCAGGA GTCGGCCAGGATCGAGGACATGAGGAAGCGGCATGTCGAGGTCTCCCAGGCCCCCTTGCCCCCCGCCCCTGCCTACCTCTCCTCTCCCCTGGCCCTGCCC AGCCAGAGGAGGAGCCCCCCCGAGGAGCCACCTGACTTCTGCTGTCCCAAGTGCCAGTATCAGGCCCCTGATATGGACACCCTGCAGATACATGTCATGG AGTGCATTGAGTAGGGCCGGCCAGTGCAAGGCCACTGCCTGCCGAGGACGTGCCCGGGACCGTGCAGTCTGCGCTTTCCTCTCCCGCCTGCCTAGCCCAG GATGAAGGGCTGGGTGGCCACAACTGGGATGCCACCTGGAGCCCCACCCAGGAGCTGGCCGCGGCACCTTACGCTTCAGCTGTTGATCCGCTGGTCCCCT CTTTTGGGGTAGATGCGGCCCCGATCAGGCCTGACTCGCTGCTCTTTTTGTTCCCTTCTGTCTGCTCGAACCACTTGCCTCGGGCTAATCCCTCCCTCTT CCTCCACCCGGCACTGGGGAAGTCAAGAATGGGGCCTGGGGCTCTCAGGGAGAACTGCTTCCCCTGGCAGAGCTGGGTGGCAGCTCTTCCTCCCACCGGA CACCGACCCGCCCGCTGCTGTGCCCTGGGAGTGCTGCCCTCTTACCATGCACACGGGTGCTCTCCTTTTGGGCTGCATGCTATTCCATTTTGCAGCCAGA CCGATGTGTATTTAACCAGTCACTATTGATGGACATTTGGGTTGTTTCCCATCTTTTTGTTACCATAAATAATGGCATAGTAAAAATCCTTGTGCATTA

IKBKG.F12 (forward primer)
IKBKG.R10 (reverse primer)
Single exon skipping

Frame 0

VRGSSRPYWAVSS*FLPRRGRGGRAPRG*TPPS ERRGGV*AVTPCKVAGVLIITELPRACRAWRTQT SLRSGMRPYRGLTLLAGFPSSRGRGLCFYFRIP QL*HRKPEAW*GRATAKLGLSRSAGALPAFTVR RSHPSHV*RTLLTALALLDE*APLEEPTV*DGAA QWWPGSRSGRTGRRVSSGEASHAAPAFRTGRS* DPPALPGGESRAPRCHPAEQPDSAGALRGASAF PSQPEGGEGVPHVQVPGGQETGGETRPGEARSEE AEGAGSAGGGAPEEMPAADG*GQGLCESPGDVL ARGAAGEPESLGGCH*GMPGSGGSGPGGQRAGA AAGE*ARGAAAAAQRAGGPAAHAGPERGGRAPHG APGRLGGEEEAGPVAGGLSPALPRIRQPHQEQR GGQ*AEARNAAGRSQTAAPAGRGGPGGQTGGDR *AEGGGRAAQDCDGDRSGAEGPGGYLQGGLPG*E AGPGEAGREEGAPAGAAGAAAEGVQQTEGQLSG VGQDRGHEEAACRGLPGPLAPRPCLPLLSPGPA QPEEEPPRGAT*LLLSQVPVSGP*YGHPADTCHG VH*VGPASARPLPAEDVPGTVQSALSSPACLAQ DEGLGGHNWDATWSPTQELAAAPYASAVDPLVP SFGVDAAPIRPDSLLFLFPSVCSNHLPRANPSLF LHPALGKSRMGPGALRENCFPWQSWVAALPPTG HRPARCCALGVLPSYHAHGCSPFGLHAIPFCSQ TDVYLTSHY*WTFGLFPIFLLP*IMA**KSLCI

Frame 1

FAGQAGPIGQSLLDSSPGEGGAAERPEARRRRP RDEGACRR*RPAKWPACLSLPSFRGPAEPGGLR LLSGAGCGPTAASHFSPASRVLGGGACVFTSGSH SYDTGSRKRGREGRPRNWDFLGAPGPYQRSQSA APTLLTSDGLC*QPLPCWMNRHLWKSQLCEMVQ PSGGPAADQDVLGEESPLGKPAMLHLPSEQGAPE TLQRCLEENQELRDAIRQSNQILRERCEELLHF QASQREEKEFLMCKFQEARKLVERLGLEKLDLK RQKEQALREVEHLKRCQQQMAEDKASVKAQVTSL LGELQESQSRLEAATKECQALEGRARAASEQAR QLESEREALQQQHSVQVDQLRMQGQSVEAALRM ERQAASEEKRKLAQLQVAYHQLFQEYDNHIKSSV VGSERKRGMQLEDLKQQLQQAEEALVAKQEVID KLKEEAEQHKIVMETVPVLKAQADIYKADFQAE RQAREKLAEKKELLQEQLEQLQREYSKLKASCQE SARIEDMRKRHVEVSQAPLPPAPAYLSSPLALP SQRRSPPEEPPDFCCPKCQYQAPDMDTLQIHVM ECIE*GRPVQGHCLPRTCPGPCSLRFPLPPA*PR MKGWVATTGMPPGAPPRSWPRHLTLQLLIRWSP LLG*MRPRSGLTRCSFCSLLSARTTCLGLIPPS SSTRHWGSQEWGLGLSGRTASPGRAGWQLFLPPD TDPPAAVPWECCPLTMHTGALLLGCMLFHFAAR PMCI*PVTIDGHLGCFPSFCYHK*WHSKNPCAL

Frame 2

SRVKPALLGSLFLIPPPERAGRPSAPRLDAAVR ETRGRVGGDALQSGRRAYHYRASAGLQSLADSD FSPERDAALPRPHTSRRLPEFSGAGLVFLLPDP TAMTPEAGSVVGKGDRETGTFSERRGPTSVHSPP LPPFSRLTDSADSPCPVG*IGTSGRANCVRWCS PVVARQQIRTYWAKSLLWGSQPCCTCLQNRALL RPSSAAWRRIKSSEMPSGRATRFCGSAARSFCIS KPARGRRRSSSCASSRRPGNWWRDSAWRSSI*R GRRSRLCGRWST*RDASSRWLRTRPL*KPR*RP CSGSCRRARVAWRLPLRNARLWRVGPGRPASRRG SWRVSARRCSSSTACRWTSCACRARAWRPRSAW SARPPRRRRGSWPSCRWPITSSSKNTTTTSRAA WWAVSGSEECSWKISNSSSSRPRRPWWPNRR*SI S*RRRPSSTRL*WRPFRC*RPRRISTRRTSRLR GRPGRSWPRRRSSCRSSWSSCRGSTAN*RPAVR SRPGSRT*GSGMSRSPRPPCPPPLPTSPLPWPCP ARGGAPPRSHLTSAVPSASIRPLIWTPCRYMSW SALSRAGQCKATACRGRARDRAVCAFLSRLPSP G*RAGWPQLGCHLEPHPGAGRGTLRFSC*SAGPL FWGRCGPDQA*LAALFVPFCLLEPLASG*SLPL PPPGTGEVKNGAWGSQGELLPLAELGGSSSSHR TPTRPLLCPGSAALLPCTRVLSFWAACYSILQPD RCVFNQSLLMDIWVVSHLFVTINNGIVKILVH

Sequence IKBKG.bAug05

CCTGAACGCCCATCAAGCCCATGGCCCTTGTGATCCAGGTGGGGAAACTAAGGCCCAGAGAAGTGAGGACCCCGCAGACTATCAATCCCAGTCTCTTCCC CTCACTCCCTGTGAAGCTCTCCAGCATCATCGAGGTCCCATCAGCCCTTGCCCTGTTGGATGAATAGGCACCTCTGGAAGAGCCAACTGTGTGAGATGGT GCAGCCCAGTGGTGGCCCGGCAGCAGATCAGGACGTACTGGGCGAAGAGTCTCCTCTGGGGAAGCCAGCCATGCTGCACCTGCCTTCAGAACAGGGCGCT CCTGAGACCCTCCAGCGCTGCCTGGAGGAGAATCAAGAGCTCCGAGATGCCATCCGGCAGAGCAACCAGATTCTGCGGGAGCGCTGCGAGGAGCTTCTGC ATTTCCAAGCCAGCCAGAGGGAGGAGAAGGAGTTCCTCATGTGCAAGTTCCAGGAGGCCAGGAAACTGGTGGAGAGACTCGGCCTGGAGAAGCTCGATCT GAAGAGGCAGAAGGAGCAGGCTCTGCGGGAGGTGGAGCACCTGAAGAGATGCCAGCAGCAGATGGCTGAGGACAAGGCCTCTGTGAAAGCCCAGGTGACG TCCTTGCTCGGGGAGCTGCAGGAGAGCCAGAGTCGCTTGGAGGCTGCCACTAAGGAATGCCAGGCTCTGGAGGGTCGGGCCCGGGCGGCCAGCGAGCAGG CGCGGCAGCTGGAGAGTGAGCGCGAGGCGCTGCAGCAGCAGCACAGCGTGCAGGTGGACCAGCTGCGCATGCAGGGCCAGAGCGTGGAGGCCGCGCTCCG CATGGAGCGCCAGGCCGCCTCGGAGGAGAAGAGGAAGCTGGCCCAGTTGCAGGTGGCCTATCACCAGCTCTTCCAAGAATACGACAACCACATCAAGAGC AGCGTGGTGGGCAGTGAGCGGAAGCGAGGAATGCAGCTGGAAGATCTCAAACAGCAGCTCCAGCAGGCCGAGGAGGCCCTGGTGGCCAAACAGGAGGTGA TCGATAAGCTGAAGGAGGAGGCCGAGCAGCACAAGATTGTGATGGAGACCGTTCCGGTGCTGAAGGCCCAGGCGGATATCTACAAGGCGGACTTCCAGGC TGAGAGGCAGGCCCGGGAGAAGCTGGCCGAGAAGAAGGAGCTCCTGCAGGAGCAGCTGGAGCAGCTGCAGAGGGAGTACAGCAAACTGAAGGCCAGCTGT CAGGAGTCGGCCAGGATCGAGGACATGAGGAAGCGGCATGTCGAGGTCTCCCAGGCCCCCTTGCCCCCCGCCCCTGCCTACCTCTCCTCTCCCCTGGCCC TGCCCAGCCAGAGGAGGAGCCCCCCCGAGGAGCCACCTGACTTCTGCTGTCCCAAGTGCCAGTATCAGGCCCCTGATATGGACACCCTGCAGATACATGT CATGGAGTGCATTGAGTAGGGCCGGCCAGTGCAAGGCCACTGCCTGCCGAGGACGTGCCCGGGACCGTGCAGTCTGCGCTTTCCTCTCCCGCCTGCCTAG CCCAGGATGAAGGGCTGGGTGGCCACAACTGGGATGCCACCTGGAGCCCCACCCAGGAGCTGGCCGCGGCACCTTACGCTTCAGCTGTTGATCCGCTGGT CCCCTCTTTTGGGGTAGATGCGGCCCCGATCAGGCCTGACTCGCTGCTCTTTTTGTTCCCTTCTGTCTGCTCGAACCACTTGCCTCGGGCTAATCCCTCC CTCTTCCTCCACCCGGCACTGGGGAAGTCAAGAATGGGGCCTGGGGCTCTCAGGGAGAACTGCTTCCCCTGGCAGAGCTGGGTGGCAGCTCTTCCTCCCA CCGGACACCGACCCGCCCGCTGCTGTGCCCTGGGAGTGCTGCCCTCTTACCATGCACACGGGTGCTCTCCTTTTGGGCTGCATGCTATTCCATTTTGCAG CCAGACCGATGTGTATTTAACCAGTCACTATTGATGGACATTTGGGTTGTTTCCCATCTTTTTGTTACCATAAATAATGGCATAGTAAAAATCCTTGTGC ATTA

IKBKG.F12 (forward primer)
IKBKG.R10 (reverse primer)
Single exon skipping

Frame 0

PERPSSPWPL*SRWGN*GPEK*GPRRLSIPVSS PHSL*SSPASSRSHQPLPCWMNRHLWKSQLCEMV QPSGGPAADQDVLGEESPLGKPAMLHLPSEQGA PETLQRCLEENQELRDAIRQSNQILRERCEELL HFQASQREEKEFLMCKFQEARKLVERLGLEKLDL KRQKEQALREVEHLKRCQQQMAEDKASVKAQVT SLLGELQESQSRLEAATKECQALEGRARAASEQ ARQLESEREALQQQHSVQVDQLRMQGQSVEAALR MERQAASEEKRKLAQLQVAYHQLFQEYDNHIKS SVVGSERKRGMQLEDLKQQLQQAEEALVAKQEV IDKLKEEAEQHKIVMETVPVLKAQADIYKADFQA ERQAREKLAEKKELLQEQLEQLQREYSKLKASC QESARIEDMRKRHVEVSQAPLPPAPAYLSSPLA LPSQRRSPPEEPPDFCCPKCQYQAPDMDTLQIHV MECIE*GRPVQGHCLPRTCPGPCSLRFPLPPA* PRMKGWVATTGMPPGAPPRSWPRHLTLQLLIRW SPLLG*MRPRSGLTRCSFCSLLSARTTCLGLIPP SSSTRHWGSQEWGLGLSGRTASPGRAGWQLFLP PDTDPPAAVPWECCPLTMHTGALLLGCMLFHFA ARPMCI*PVTIDGHLGCFPSFCYHK*WHSKNPCA L

Frame 1

LNAHQAHGPCDPGGETKAQRSEDPADYQSQSLP LTPCEALQHHRGPISPCPVG*IGTSGRANCVRW CSPVVARQQIRTYWAKSLLWGSQPCCTCLQNRAL LRPSSAAWRRIKSSEMPSGRATRFCGSAARSFC ISKPARGRRRSSSCASSRRPGNWWRDSAWRSSI *RGRRSRLCGRWST*RDASSRWLRTRPL*KPR*R PCSGSCRRARVAWRLPLRNARLWRVGPGRPASR RGSWRVSARRCSSSTACRWTSCACRARAWRPRS AWSARPPRRRRGSWPSCRWPITSSSKNTTTTSRA AWWAVSGSEECSWKISNSSSSRPRRPWWPNRR* SIS*RRRPSSTRL*WRPFRC*RPRRISTRRTSR LRGRPGRSWPRRRSSCRSSWSSCRGSTAN*RPAV RSRPGSRT*GSGMSRSPRPPCPPPLPTSPLPWP CPARGGAPPRSHLTSAVPSASIRPLIWTPCRYM SWSALSRAGQCKATACRGRARDRAVCAFLSRLPS PG*RAGWPQLGCHLEPHPGAGRGTLRFSC*SAG PLFWGRCGPDQA*LAALFVPFCLLEPLASG*SL PLPPPGTGEVKNGAWGSQGELLPLAELGGSSSSH RTPTRPLLCPGSAALLPCTRVLSFWAACYSILQ PDRCVFNQSLLMDIWVVSHLFVTINNGIVKILV H

Frame 2

*TPIKPMALVIQVGKLRPREVRTPQTINPSLFP SLPVKLSSIIEVPSALALLDE*APLEEPTV*DG AAQWWPGSRSGRTGRRVSSGEASHAAPAFRTGR S*DPPALPGGESRAPRCHPAEQPDSAGALRGASA FPSQPEGGEGVPHVQVPGGQETGGETRPGEARS EEAEGAGSAGGGAPEEMPAADG*GQGLCESPGD VLARGAAGEPESLGGCH*GMPGSGGSGPGGQRAG AAAGE*ARGAAAAAQRAGGPAAHAGPERGGRAP HGAPGRLGGEEEAGPVAGGLSPALPRIRQPHQE QRGGQ*AEARNAAGRSQTAAPAGRGGPGGQTGGD R*AEGGGRAAQDCDGDRSGAEGPGGYLQGGLPG *EAGPGEAGREEGAPAGAAGAAAEGVQQTEGQL SGVGQDRGHEEAACRGLPGPLAPRPCLPLLSPGP AQPEEEPPRGAT*LLLSQVPVSGP*YGHPADTC HGVH*VGPASARPLPAEDVPGTVQSALSSPACL AQDEGLGGHNWDATWSPTQELAAAPYASAVDPLV PSFGVDAAPIRPDSLLFLFPSVCSNHLPRANPS LFLHPALGKSRMGPGALRENCFPWQSWVAALPP TGHRPARCCALGVLPSYHAHGCSPFGLHAIPFCS QTDVYLTSHY*WTFGLFPIFLLP*IMA**KSLC I

Sequence IKBKG.aAug05

CCTGAACGCCCATCAAGCCCATGGCCCTTGTGATCCAGGTGGGGAAACTAAGGCCCAGAGAAGTGAGGACCCCGCAGACTATCAATCCCAGTCTCTTCCC CTCACTCCCTGTGAAGCTCTCCAGCATCATCGAGGTCCCATCAGGTGGGGAAAGATGCTGTTCCAGGCGCACACTAGTCTACAAGGCCAGAGCTTTCTGG AAGGGGGCACCCTTGCCCTGTTGGATGAATAGGCACCTCTGGAAGAGCCAACTGTGTGAGATGGTGCAGCCCAGTGGTGGCCCGGCAGCAGATCAGGACG TACTGGGCGAAGAGTCTCCTCTGGGGAAGCCAGCCATGCTGCACCTGCCTTCAGAACAGGGCGCTCCTGAGACCCTCCAGCGCTGCCTGGAGGAGAATCA AGAGCTCCGAGATGCCATCCGGCAGAGCAACCAGATTCTGCGGGAGCGCTGCGAGGAGCTTCTGCATTTCCAAGCCAGCCAGAGGGAGGAGAAGGAGTTC CTCATGTGCAAGTTCCAGGAGGCCAGGAAACTGGTGGAGAGACTCGGCCTGGAGAAGCTCGATCTGAAGAGGCAGAAGGAGCAGGCTCTGCGGGAGGTGG AGCACCTGAAGAGATGCCAGCAGCAGATGGCTGAGGACAAGGCCTCTGTGAAAGCCCAGGTGACGTCCTTGCTCGGGGAGCTGCAGGAGAGCCAGAGTCG CTTGGAGGCTGCCACTAAGGAATGCCAGGCTCTGGAGGGTCGGGCCCGGGCGGCCAGCGAGCAGGCGCGGCAGCTGGAGAGTGAGCGCGAGGCGCTGCAG CAGCAGCACAGCGTGCAGGTGGACCAGCTGCGCATGCAGGGCCAGAGCGTGGAGGCCGCGCTCCGCATGGAGCGCCAGGCCGCCTCGGAGGAGAAGAGGA AGCTGGCCCAGTTGCAGGTGGCCTATCACCAGCTCTTCCAAGAATACGACAACCACATCAAGAGCAGCGTGGTGGGCAGTGAGCGGAAGCGAGGAATGCA GCTGGAAGATCTCAAACAGCAGCTCCAGCAGGCCGAGGAGGCCCTGGTGGCCAAACAGGAGGTGATCGATAAGCTGAAGGAGGAGGCCGAGCAGCACAAG ATTGTGATGGAGACCGTTCCGGTGCTGAAGGCCCAGGCGGATATCTACAAGGCGGACTTCCAGGCTGAGAGGCAGGCCCGGGAGAAGCTGGCCGAGAAGA AGGAGCTCCTGCAGGAGCAGCTGGAGCAGCTGCAGAGGGAGTACAGCAAACTGAAGGCCAGCTGTCAGGAGTCGGCCAGGATCGAGGACATGAGGAAGCG GCATGTCGAGGTCTCCCAGGCCCCCTTGCCCCCCGCCCCTGCCTACCTCTCCTCTCCCCTGGCCCTGCCCAGCCAGAGGAGGAGCCCCCCCGAGGAGCCA CCTGACTTCTGCTGTCCCAAGTGCCAGTATCAGGCCCCTGATATGGACACCCTGCAGATACATGTCATGGAGTGCATTGAGTAGGGCCGGCCAGTGCAAG GCCACTGCCTGCCGAGGACGTGCCCGGGACCGTGCAGTCTGCGCTTTCCTCTCCCGCCTGCCTAGCCCAGGATGAAGGGCTGGGTGGCCACAACTGGGAT GCCACCTGGAGCCCCACCCAGGAGCTGGCCGCGGCACCTTACGCTTCAGCTGTTGATCCGCTGGTCCCCTCTTTTGGGGTAGATGCGGCCCCGATCAGGC CTGACTCGCTGCTCTTTTTGTTCCCTTCTGTCTGCTCGAACCACTTGCCTCGGGCTAATCCCTCCCTCTTCCTCCACCCGGCACTGGGGAAGTCAAGAAT GGGGCCTGGGGCTCTCAGGGAGAACTGCTTCCCCTGGCAGAGCTGGGTGGCAGCTCTTCCTCCCACCGGACACCGACCCGCCCGCTGCTGTGCCCTGGGA GTGCTGCCCTCTTACCATGCACACGGGTGCTCTCCTTTTGGGCTGCATGCTATTCCATTTTGCAGCCAGACCGATGTGTATTTAACCAGTCACTATTGAT GGACATTTGGGTTGTTTCCCATCTTTTTGTTACCATAAATAATGGCATAGTAAAAATCCTTGTGCATTA

IKBKG.F12 (forward primer)
IKBKG.R10 (reverse primer)
Single exon skipping

Frame 0

PERPSSPWPL*SRWGN*GPEK*GPRRLSIPVSS PHSL*SSPASSRSHQVGKDAVPGAH*STRPELSG RGHPCPVG*IGTSGRANCVRWCSPVVARQQIRT YWAKSLLWGSQPCCTCLQNRALLRPSSAAWRRI KSSEMPSGRATRFCGSAARSFCISKPARGRRRSS SCASSRRPGNWWRDSAWRSSI*RGRRSRLCGRW ST*RDASSRWLRTRPL*KPR*RPCSGSCRRARV AWRLPLRNARLWRVGPGRPASRRGSWRVSARRCS SSTACRWTSCACRARAWRPRSAWSARPPRRRRG SWPSCRWPITSSSKNTTTTSRAAWWAVSGSEEC SWKISNSSSSRPRRPWWPNRR*SIS*RRRPSSTR L*WRPFRC*RPRRISTRRTSRLRGRPGRSWPRR RSSCRSSWSSCRGSTAN*RPAVRSRPGSRT*GS GMSRSPRPPCPPPLPTSPLPWPCPARGGAPPRSH LTSAVPSASIRPLIWTPCRYMSWSALSRAGQCK ATACRGRARDRAVCAFLSRLPSPG*RAGWPQLG CHLEPHPGAGRGTLRFSC*SAGPLFWGRCGPDQA *LAALFVPFCLLEPLASG*SLPLPPPGTGEVKN GAWGSQGELLPLAELGGSSSSHRTPTRPLLCPG SAALLPCTRVLSFWAACYSILQPDRCVFNQSLLM DIWVVSHLFVTINNGIVKILVH

Frame 1

LNAHQAHGPCDPGGETKAQRSEDPADYQSQSLP LTPCEALQHHRGPIRWGKMLFQAHTSLQGQSFL EGGTLALLDE*APLEEPTV*DGAAQWWPGSRSGR TGRRVSSGEASHAAPAFRTGRS*DPPALPGGES RAPRCHPAEQPDSAGALRGASAFPSQPEGGEGV PHVQVPGGQETGGETRPGEARSEEAEGAGSAGGG APEEMPAADG*GQGLCESPGDVLARGAAGEPES LGGCH*GMPGSGGSGPGGQRAGAAAGE*ARGAA AAAQRAGGPAAHAGPERGGRAPHGAPGRLGGEEE AGPVAGGLSPALPRIRQPHQEQRGGQ*AEARNA AGRSQTAAPAGRGGPGGQTGGDR*AEGGGRAAQ DCDGDRSGAEGPGGYLQGGLPG*EAGPGEAGREE GAPAGAAGAAAEGVQQTEGQLSGVGQDRGHEEA ACRGLPGPLAPRPCLPLLSPGPAQPEEEPPRGA T*LLLSQVPVSGP*YGHPADTCHGVH*VGPASAR PLPAEDVPGTVQSALSSPACLAQDEGLGGHNWD ATWSPTQELAAAPYASAVDPLVPSFGVDAAPIR PDSLLFLFPSVCSNHLPRANPSLFLHPALGKSRM GPGALRENCFPWQSWVAALPPTGHRPARCCALG VLPSYHAHGCSPFGLHAIPFCSQTDVYLTSHY* WTFGLFPIFLLP*IMA**KSLCI

Frame 2

*TPIKPMALVIQVGKLRPREVRTPQTINPSLFP SLPVKLSSIIEVPSGGERCCSRRTLVYKARAFW KGAPLPCWMNRHLWKSQLCEMVQPSGGPAADQD VLGEESPLGKPAMLHLPSEQGAPETLQRCLEENQ ELRDAIRQSNQILRERCEELLHFQASQREEKEF LMCKFQEARKLVERLGLEKLDLKRQKEQALREV EHLKRCQQQMAEDKASVKAQVTSLLGELQESQSR LEAATKECQALEGRARAASEQARQLESEREALQ QQHSVQVDQLRMQGQSVEAALRMERQAASEEKR KLAQLQVAYHQLFQEYDNHIKSSVVGSERKRGMQ LEDLKQQLQQAEEALVAKQEVIDKLKEEAEQHK IVMETVPVLKAQADIYKADFQAERQAREKLAEK KELLQEQLEQLQREYSKLKASCQESARIEDMRKR HVEVSQAPLPPAPAYLSSPLALPSQRRSPPEEP PDFCCPKCQYQAPDMDTLQIHVMECIE*GRPVQ GHCLPRTCPGPCSLRFPLPPA*PRMKGWVATTGM PPGAPPRSWPRHLTLQLLIRWSPLLG*MRPRSG LTRCSFCSLLSARTTCLGLIPPSSSTRHWGSQE WGLGLSGRTASPGRAGWQLFLPPDTDPPAAVPWE CCPLTMHTGALLLGCMLFHFAARPMCI*PVTID GHLGCFPSFCYHK*WHSKNPCAL