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Sequences

Sequence WISP2.dAug05

GGAGACCTTGGGTCAGCTCTGCAAAGGGGTTGTTTACTGAATGCTTCTGGTATCTGCAGCCCCGCTGGGGTTCGGACAGGGGGTCTGGAATAGGGCTTCA
CAACTACAGCAGGGCTAGGACCCTCTTGGGCTGGAAGGAGCTGGAGAGGCCTCTGCAAGCTGTCACAGGCTCTTGGTGCTGGCACTGGCTCAGGCTTTCA
CACACACACACGCGCACACACACACACACACACACACGGACAGGCACCCCCTTGGTGGCCTTCACAGTTTCACCTTCAGGTAAATGGGCTCATCCTTTGA
GCCATGAGGATGGGAAGCGAAGCAAGGAATGAAAAAGCTAGTGTGTTTGTGTGTGTGTGTGTGTGTGAGCGCGCGCGCGCGCGCGCGTGTGTACTCGTGC
GTGTGCCTGTGTGTGCCTGGGAGTGACCTCACAGCTGCCGGAACATAAAGACTCACAGGTCCGCCTCCCAGGCTCAAAGCTGGCTCTGCAGGGGACATGA
GAGGCACACCGAAGACCCACCTCCTGGCCTTCTCCCTCCTCTGCCTCCTCTCAAAGGTGCGTACCCAGCTGTGCCCGACACCATGTACCTGCCCCTGGCC
ACCTCCCCGATGCCCGCTGGGAGTACCCCTGGTGCTGGATGGCTGTGGCTGCTGCCGGGTATGTGCACGGCGGCTGGGGGAGCCCTGCGACCAACTCCAC
GTCTGCGACGCCAGCCAGGGCCTGGTCTGCCAGCCCGGGGCAGGACCCGGTGGCCGGGGGGCCCTGTGCCTCTGTAAGCAGGACCCCAGTTTTCTGGCCT
TGTCTCTTCCCTGCCCCCTGGTGTCCCCTGCCCAGAATGGAGCACGGCCTGGGGACCCTGCTCGACCACCTGTGGGCTGGGCATGGCCACCCGGGTGTCC
AACCAGAACCGCTTCTGCCGACTGGAGACCCAGCGCCGCCTGTGCCTGTCCAGGCCCTGCCCACCCTCCAGGGGTCGCAGTCCACAAAACAGTGCCTTCT
AGAGCCGGGCTGGGAATGGGGACACGGTGTCCACCATCCCCAGCTGGTGGCCCTGTGCCTGGGCCCTGGGCTGATGGAAGATGGTCCGTGCCCAGGCCCT
TGGCTGCAGGCAACACTTTAGCTTGGGTCCACCATGCAGAACACCAATATTAACACGCTGCCTGGTCTGTCTGGATCCCGAGGTATGGCAGAGGTGCAAG
ACCTAGTCCCCTTTCCTCTAACTCACTGCCTAGGAGGCTGGCCAAGGTGTCCAGGGTCCTCTAGCCCACTCCCTGCCTACACACACAGCCTATATCAAAC
ATGCACACGGGCGAGCTTTCTCTCCGACTTCCCCTGGGCAAGAGATGGGACAAGCAGTCCCTTAATATTGAGGCTGCAGCAGGTGCTGGGCTGGACTGGC
CATTTTTCTGGGGGTAGGATGAAGAGAAGGCACACAGAGATTCTGGATCTCCTGCTGCCTTTTCTGGAGTTTGTAAAATTGTTCCTGAATACAAGCCTAT
GCGTGATCTTGTGTGTCAGGCCTGTGTTTTCTGAGAAAGCCCTCCCATTATCCGGGGCAGAATTCAGGGGCAGAGTTCTCTGTGAACCTCACAAGGTGAA
ATCACATGGTCTTAAAGAAAGCCAGAAGACAGGCCTTCAAGTGATCCAAGAAACCCAATTCATATTAATAATAATTAACTTTTTTATTTATTTATTTTTT
TGAGATGGAGTTTTGCTCTTGTTGCCCAGGCTGGAGCTGGAGTACAATAGCACGATCTCGACTCACTGCAGCCTCCCGGGTTCAAGTGATTCTCCTGCCT
CAAGACTCCTGAGTAGCTGGGATTATAGGCGCATGCCACCATGCTCGGCTTTTTTTTTTTTTTTAAATATATTTTTAGTAGAGATGGGGTTTAGCCATGA
TGGTCAGGCTGGTCTCGAACTCCTGACCTCAAGTGATCCGCCCGCCGCCTCGGCCTCCCAAAGTGCTGGGATTACAGGCGTGAGCTACTGCGCCCAGCCA
ATAATTAACATTGCTTGAGCGCCTGCTATGTGCTAGGCACTAGCACTTTATACAGACCAGTGCTTCCCAGCGTTTTTGACCAGAGAAGAGCAGGGAGAAT
GAAGGCTGCCTCCCAGGGACTGGGGAGCATAGCCTGAAATCAACTAACCCAAGGGTGAAATTGTTCGAAGTCTCATGTTTTATTGTTTACAAACAAAACA
AAAC
  • WISP2.F1 (forward primer)
  • WISP2.R1 (reverse primer)
  • Single exon skipping

Frame 0

GDLGSALQRGCLLNASGICSPAGVRTGGLE*GF
TTTAGLGPSWAGRSWRGLCKLSQALGAGTGSGFH
THTRAHTHTHTHGQAPPWWPSQFHLQVNGLIL*
AMRMGSEARNEKASVFVCVCVCERARARACVLV
RVPVCAWE*PHSCRNIKTHRSASQAQSWLCRGHE
RHTEDPPPGLLPPLPPLKGAYPAVPDTMYLPLA
TSPMPAGSTPGAGWLWLLPGMCTAAGGALRPTP
RLRRQPGPGLPARGRTRWPGGPVPL*AGPQFSGL
VSSLPPGVPCPEWSTAWGPCSTTCGLGMATRVS
NQNRFCRLETQRRLCLSRPCPPSRGRSPQNSAF
*SRAGNGDTVSTIPSWWPCAWALG*WKMVRAQAL
GCRQHFSLGPPCRTPILTRCLVCLDPEVWQRCK
T*SPFL*LTA*EAGQGVQGPLAHSLPTHTAYIK
HAHGRAFSPTSPGQEMGQAVP*Y*GCSRCWAGLA
IFLGVG*REGTQRFWISCCLFWSL*NCS*IQAY
A*SCVSGLCFLRKPSHYPGQNSGAEFSVNLTR*
NHMVLKKARRQAFK*SKKPNSY***LTFLFIYFF
EMEFCSCCPGWSWSTIARSRLTAASRVQVILLP
QDS*VAGIIGACHHARLFFFF*IYF**RWGLAM
MVRLVSNS*PQVIRPPPRPPKVLGLQA*ATAPSQ
*LTLLERLLCARH*HFIQTSASQRF*PEKSREN
EGCLPGTGEHSLKSTNPRVKLFEVSCFIVYKQN
K

Frame 1

ETLGQLCKGVVY*MLLVSAAPLGFGQGVWNRAS
QLQQG*DPLGLEGAGEASASCHRLLVLALAQAF
THTHAHTHTHTHTDRHPLGGLHSFTFR*MGSSFE
P*GWEAKQGMKKLVCLCVCVCVSARARARVYSC
VCLCVPGSDLTAAGT*RLTGPPPRLKAGSAGDM
RGTPKTHLLAFSLLCLLSKVRTQLCPTPCTCPWP
PPRCPLGVPLVLDGCGCCRVCARRLGEPCDQLH
VCDASQGLVCQPGAGPGGRGALCLCKQDPSFLA
LSLPCPLVSPAQNGARPGDPARPPVGWAWPPGCP
TRTASADWRPSAACACPGPAHPPGVAVHKTVPS
RAGLGMGTRCPPSPAGGPVPGPWADGRWSVPRP
LAAGNTLAWVHHAEHQY*HAAWSVWIPRYGRGAR
PSPLSSNSLPRRLAKVSRVL*PTPCLHTQPISN
MHTGELSLRLPLGKRWDKQSLNIEAAAGAGLDW
PFFWG*DEEKAHRDSGSPAAFSGVCKIVPEYKPM
RDLVCQACVF*ESPPIIRGRIQGQSSL*TSQGE
ITWS*RKPEDRPSSDPRNPIHINNN*LFYLFIF
LRWSFALVAQAGAGVQ*HDLDSLQPPGFK*FSCL
KTPE*LGL*AHATMLGFFFFFKYIFSRDGV*P*
WSGWSRTPDLK*SARRLGLPKCWDYRRELLRPA
NN*HCLSACYVLGTSTLYRPVLPSVFDQRRAGRM
KAASQGLGSIA*NQLTQG*NCSKSHVLLFTNKT
K

Frame 2

RPWVSSAKGLFTECFWYLQPRWGSDRGSGIGLH
NYSRARTLLGWKELERPLQAVTGSWCWHWLRLS
HTHTRTHTHTHTRTGTPLVAFTVSPSGKWAHPL
SHEDGKRSKE*KS*CVCVCVCV*ARARARVCTRA
CACVCLGVTSQLPEHKDSQVRLPGSKLALQGT*
EAHRRPTSWPSPSSASSQRCVPSCARHHVPAPG
HLPDARWEYPWCWMAVAAAGYVHGGWGSPATNST
SATPARAWSASPGQDPVAGGPCASVSRTPVFWP
CLFPAPWCPLPRMEHGLGTLLDHLWAGHGHPGV
QPEPLLPTGDPAPPVPVQALPTLQGSQSTKQCLL
EPGWEWGHGVHHPQLVALCLGPGLMEDGPCPGP
WLQATL*LGSTMQNTNINTLPGLSGSRGMAEVQ
DLVPFPLTHCLGGWPRCPGSSSPLPAYTHSLYQT
CTRASFLSDFPWARDGTSSPLILRLQQVLGWTG
HFSGGRMKRRHTEILDLLLPFLEFVKLFLNTSL
CVILCVRPVFSEKALPLSGAEFRGRVLCEPHKVK
SHGLKESQKTGLQVIQETQFILIIINFFIYLFF
*DGVLLLLPRLELEYNSTISTHCSLPGSSDSPA
SRLLSSWDYRRMPPCSAFFFFLNIFLVEMGFSHD
GQAGLELLTSSDPPAASASQSAGITGVSYCAQP
IINIA*APAMC*ALALYTDQCFPAFLTREEQGE
*RLPPRDWGA*PEIN*PKGEIVRSLMFYCLQTKQ
N

Sequence WISP2.eAug05

GGCTGCTGCCGGGTATGTGCACGGCGGCTGGGGGAGCCCTGCGACCAACTCCACGTCTGCGACGCCAGCCAGGGCCTGGTCTGCCAGCCCGGGGCAGGAC
CCGGTGGCCGGGGGGCCCTGTGCCTCTGTAAGCAGTGGCAGAGGACGACAGCAGCTGTGAGGTGAACGGCCGCCTGTATCGGGAAGGGGAGACCTTCCAG
CCCCACTGCAGCATCCGCTGCCGCTGCGAGGACGGCGGCTTCACCTGCGTGCCGCTGTGCAGCGAGGATGTGCGGCTGCCCAGCTGGGACTGCCCCCACC
CCAGGAGGGTCGAGGTCCTGGGCAAGTGCTGCCCTGAGTGGGTGTGCGGCCAAGGAGGGGGACTGGGGACCCAGCCCCTTCCAGCCCAAGGACCCCAGTT
TTCTGGCCTTGTCTCTTCCCTGCCCCCTGGTGTCCCCTGCCCAGAATGGAGCACGGCCTGGGGACCCTGCTCGACCACCTGTGGGCTGGGCATGGCCACC
CGGGTGTCCAACCAGAACCGCTTCTGCCGACTGGAGACCCAGCGCCGCCTGTGCCTGTCCAGGCCCTGCCCACCCTCCAGGGGTCGCAGTCCACAAAACA
GTGCCTTCTAGAGCCGGGCTGGGAATGGGGACACGGTGTCCACCATCCCCAGCTGGTGGCCCTGTGCCTGGGCCCTGGGCTGATGGAAGATGGTCCGTGC
CCAGGCCCTTGGCTGCAGGCAACACTTTAGCTTGGGTCCACCATGCAGAACACCAATATTAACACGCTGCCTGGTCTGTCTGGATCCCGAGGTATGGCAG
AGGTGCAAGACCTAGTCCCCTTTCCTCTAACTCACTGCCTAGGAGGCTGGCCAAGGTGTCCAGGGTCCTCTAGCCCACTCCCTGCCTACACACACAGCCT
ATATCAAACATGCACACGGGCGAGCTTTCTCTCCGACTTCCCCTGGGCAAGAGATGGGACAAGCAGTCCCTTAATATTGAGGCTGCAGCAGGTGCTGGGC
TGGACTGGCCATTTTTCTGGGGGTAGGATGAAGAGAAGGCACACAGAGATTCTGGATCTCCTGCTGCCTTTTCTGGAGTTTGTAAAATTGTTCCTGAATA
CAAGCCTATGCGTGATCTTGTGTGTCAGGCCTGTGTTTTCTGAGAAAGCCCTCCCATTATCCGGGGCAGAATTCAGGGGCAGAGTTCTCTGTGAACCTCA
CAAGGTGAAATCACATGGTCTTAAAGAAAGCCAGAAGACAGGCCTTCAAGTGATCCAAGAAACCCAATTCATATTAATAATAATTAACTTTTTTATTTAT
TTATTTTTTTGAGATGGAGTTTTGCTCTTGTTGCCCAGGCTGGAGCTGGAGTACAATAGCACGATCTCGACTCACTGCAGCCTCCCGGGTTCAAGTGATT
CTCCTGCCTCAAGACTCCTGAGTAGCTGGGATTATAGGCGCATGCCACCATGCTCGGCTTTTTTTTTTTTTTTAAATATATTTTTAGTAGAGATGGGGTT
TAGCCATGATGGTCAGGCTGGTCTCGAACTCCTGACCTCAAGTGATCCGCCCGCCGCCTCGGCCTCCCAAAGTGCTGGGATTACAGGCGTGAGCTACTGC
GCCCAGCCAATAATTAACATTGCTTGAGCGCCTGCTATGTGCTAGGCACTAGCACTTTATACAGACCAGTGCTTCCCAGCGTTTTTGACCAGAGAAGAGC
AGGGAGAATGAAGGCTGCCTCCCAGGGACTGGGGAGCATAGCCTGAAATCAACTAACCCAAGGGTGAAATTGTTCGAAGTCTCATGTTTTATTGTTTACA
AACAAAACAAAAC
  • WISP2.F1 (forward primer)
  • WISP2.R1 (reverse primer)
  • Single exon skipping

Frame 0

GCCRVCARRLGEPCDQLHVCDASQGLVCQPGAG
PGGRGALCLCKQWQRTTAAVR*TAACIGKGRPSS
PTAASAAAARTAASPACRCAARMCGCPAGTAPT
PGGSRSWASAALSGCAAKEGDWGPSPFQPKDPS
FLALSLPCPLVSPAQNGARPGDPARPPVGWAWPP
GCPTRTASADWRPSAACACPGPAHPPGVAVHKT
VPSRAGLGMGTRCPPSPAGGPVPGPWADGRWSV
PRPLAAGNTLAWVHHAEHQY*HAAWSVWIPRYGR
GARPSPLSSNSLPRRLAKVSRVL*PTPCLHTQP
ISNMHTGELSLRLPLGKRWDKQSLNIEAAAGAG
LDWPFFWG*DEEKAHRDSGSPAAFSGVCKIVPEY
KPMRDLVCQACVF*ESPPIIRGRIQGQSSL*TS
QGEITWS*RKPEDRPSSDPRNPIHINNN*LFYL
FIFLRWSFALVAQAGAGVQ*HDLDSLQPPGFK*F
SCLKTPE*LGL*AHATMLGFFFFFKYIFSRDGV
*P*WSGWSRTPDLK*SARRLGLPKCWDYRRELL
RPANN*HCLSACYVLGTSTLYRPVLPSVFDQRRA
GRMKAASQGLGSIA*NQLTQG*NCSKSHVLLFT
NKTK

Frame 1

AAAGYVHGGWGSPATNSTSATPARAWSASPGQD
PVAGGPCASVSSGRGRQQL*GERPPVSGRGDLP
APLQHPLPLRGRRLHLRAAVQRGCAAAQLGLPPP
QEGRGPGQVLP*VGVRPRRGTGDPAPSSPRTPV
FWPCLFPAPWCPLPRMEHGLGTLLDHLWAGHGH
PGVQPEPLLPTGDPAPPVPVQALPTLQGSQSTKQ
CLLEPGWEWGHGVHHPQLVALCLGPGLMEDGPC
PGPWLQATL*LGSTMQNTNINTLPGLSGSRGMA
EVQDLVPFPLTHCLGGWPRCPGSSSPLPAYTHSL
YQTCTRASFLSDFPWARDGTSSPLILRLQQVLG
WTGHFSGGRMKRRHTEILDLLLPFLEFVKLFLN
TSLCVILCVRPVFSEKALPLSGAEFRGRVLCEPH
KVKSHGLKESQKTGLQVIQETQFILIIINFFIY
LFF*DGVLLLLPRLELEYNSTISTHCSLPGSSD
SPASRLLSSWDYRRMPPCSAFFFFLNIFLVEMGF
SHDGQAGLELLTSSDPPAASASQSAGITGVSYC
AQPIINIA*APAMC*ALALYTDQCFPAFLTREE
QGE*RLPPRDWGA*PEIN*PKGEIVRSLMFYCLQ
TKQN

Frame 2

LLPGMCTAAGGALRPTPRLRRQPGPGLPARGRT
RWPGGPVPL*AVAEDDSSCEVNGRLYREGETFQ
PHCSIRCRCEDGGFTCVPLCSEDVRLPSWDCPH
PRRVEVLGKCCPEWVCGQGGGLGTQPLPAQGPQF
SGLVSSLPPGVPCPEWSTAWGPCSTTCGLGMAT
RVSNQNRFCRLETQRRLCLSRPCPPSRGRSPQN
SAF*SRAGNGDTVSTIPSWWPCAWALG*WKMVRA
QALGCRQHFSLGPPCRTPILTRCLVCLDPEVWQ
RCKT*SPFL*LTA*EAGQGVQGPLAHSLPTHTA
YIKHAHGRAFSPTSPGQEMGQAVP*Y*GCSRCWA
GLAIFLGVG*REGTQRFWISCCLFWSL*NCS*I
QAYA*SCVSGLCFLRKPSHYPGQNSGAEFSVNL
TR*NHMVLKKARRQAFK*SKKPNSY***LTFLFI
YFFEMEFCSCCPGWSWSTIARSRLTAASRVQVI
LLPQDS*VAGIIGACHHARLFFFF*IYF**RWG
LAMMVRLVSNS*PQVIRPPPRPPKVLGLQA*ATA
PSQ*LTLLERLLCARH*HFIQTSASQRF*PEKS
RENEGCLPGTGEHSLKSTNPRVKLFEVSCFIVY
KQNK

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