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Sequences

Sequence WISP2.bAug05

TGTGTGTGTGTGTGTGTGAGCGCGCGCGCGCGCGCGCGTGTGTACTCGTGCGTGTGCCTGTGTGTGCCTGGGAGTGACCTCACAGCTGCCGGAACATAAA
GACTCACAGGTCCGCCTCCCAGGCTCAAAGCTGGCTCTGCAGGGGACATGAGAGGCACACCGAAGACCCACCTCCTGGCCTTCTCCCTCCTCTGCCTCCT
CTCAAAGGTGCGTACCCAGCTGTGCCCGACACCATGTACCTGCCCCTGGCCACCTCCCCGATGCCCGCTGGGAGTACCCCTGGTGCTGGATGGCTGTGGC
TGCTGCCGGGTATGTGCACGGCGGCTGGGGGAGCCCTGCGACCAACTCCACGTCTGCGACGCCAGCCAGGGCCTGGTCTGCCAGCCCGGGGCAGGACCCG
GTGGCCGGGGGGCCCTGTGCCTCTTGGCAGAGGACGACAGCAGCTGTGAGGTGAACGGCCGCCTGTATCGGGAAGGGGAGACCTTCCAGCCCCACTGCAG
CATCCGCTGCCGCTGCGAGGACGGCGGCTTCACCTGCGTGCCGCTGTGCAGCGAGGATGTGCGGCTGCCCAGCTGGGACTGCCCCCACCCCAGGAGGGTC
GAGGTCCTGGGCAAGTGCTGCCCTGAGTGGGTGTGCGGCCAAGGAGGGGGACTGGGGACCCAGCCCCTTCCAGCCCAAGGACCCCAGTTTTCTGGCCTTG
TCTCTTCC
CTGCCCCCTGGTGTCCCCTGCCCAGAATGGAGCACGGCCTGGGGACCCTGCTCGACCACCTGTGGGCTGGGCATGGCCACCCGGGTGTCCAA
CCAGAACCGCTTCTGCCGACTGGAGACCCAGCGCCGCCTGTGCCTGTCCAGGCCCTGCCCACCCTCCAGGGGTCGCAGTCCACAAAACAGTGCCTTCTAG
AGCCGGGCTGGGAATGGGGACACGGTGTCCACCATCCCCAGCTGGTGGCCCTGTGCCTGGGCCCTGGGCTGATGGAAGATGGTCCGTGCCCAGGCCCTTG
GCTGCAGGCAACACTTTAGCTTGGGTCCACCATGCAGAACACCAATATTAACACGCTGCCTGGTCTGTCTGGATCCCGAGGTATGGCAGAGGTGCAAGAC
CTAGTCCCCTTTCCTCTAACTCACTGCCTAGGAGGCTGGCCAAGGTGTCCAGGGTCCTCTAGCCCACTCCCTGCCTACACACACAGCCTATATCAAACAT
GCACACGGGCGAGCTTTCTCTCCGACTTCCCCTGGGCAAGAGATGGGACAAGCAGTCCCTTAATATTGAGGCTGCAGCAGGTGCTGGGCTGGACTGGCCA
TTTTTCTGGGGGTAGGATGAAGAGAAGGCACACAGAGATTCTGGATCTCCTGCTGCCTTTTCTGGAGTTTGTAAAATTGTTCCTGAATACAAGCCTATGC
GTGATCTTGTGTGTCAGGCCTGTGTTTTCTGAGAAAGCCCTCCCATTATCCGGGGCAGAATTCAGGGGCAGAGTTCTCTGTGAACCTCACAAGGTGAAAT
CACATGGTCTTAAAGAAAGCCAGAAGACAGGCCTTCAAGTGATCCAAGAAACCCAATTCATATTAATAATAATTAACTTTTTTATTTATTTATTTTTTTG
AGATGGAGTTTTGCTCTTGTTGCCCAGGCTGGAGCTGGAGTACAATAGCACGATCTCGACTCACTGCAGCCTCCCGGGTTCAAGTGATTCTCCTGCCTCA
AGACTCCTGAGTAGCTGGGATTATAGGCGCATGCCACCATGCTCGGCTTTTTTTTTTTTTTTAAATATATTTTTAGTAGAGATGGGGTTTAGCCATGATG
GTCAGGCTGGTCTCGAACTCCTGACCTCAAGTGATCCGCCCGCCGCCTCGGCCTCCCAAAGTGCTGGGATTACAGGCGTGAGCTACTGCGCCCAGCCAAT
AATTAACATTGCTTGAGCGCCTGCTATGTGCTAGGCACTAGCACTTTATACAGACCAGTGCTTCCCAGCGTTTTTGACCAGAGAAGAGCAGGGAGAATGA
AGGCTGCCTCCCAGGGACTGGGGAGCATAGCCTGAAATCAACTAACCCAAGGGTGAAATTGTTCGAAGTCTCATGTTTTATTGTTTACAAACAAAACAAA
AC
  • WISP2.F1 (forward primer)
  • WISP2.R1 (reverse primer)
  • Alternative donor

Frame 0

CVCVCVSARARARVYSCVCLCVPGSDLTAAGT*
RLTGPPPRLKAGSAGDMRGTPKTHLLAFSLLCLL
SKVRTQLCPTPCTCPWPPPRCPLGVPLVLDGCG
CCRVCARRLGEPCDQLHVCDASQGLVCQPGAGP
GGRGALCLLAEDDSSCEVNGRLYREGETFQPHCS
IRCRCEDGGFTCVPLCSEDVRLPSWDCPHPRRV
EVLGKCCPEWVCGQGGGLGTQPLPAQGPQFSGL
VSS
LPPGVPCPEWSTAWGPCSTTCGLGMATRVSN
QNRFCRLETQRRLCLSRPCPPSRGRSPQNSAF*
SRAGNGDTVSTIPSWWPCAWALG*WKMVRAQAL
GCRQHFSLGPPCRTPILTRCLVCLDPEVWQRCKT
*SPFL*LTA*EAGQGVQGPLAHSLPTHTAYIKH
AHGRAFSPTSPGQEMGQAVP*Y*GCSRCWAGLA
IFLGVG*REGTQRFWISCCLFWSL*NCS*IQAYA
*SCVSGLCFLRKPSHYPGQNSGAEFSVNLTR*N
HMVLKKARRQAFK*SKKPNSY***LTFLFIYFF
EMEFCSCCPGWSWSTIARSRLTAASRVQVILLPQ
DS*VAGIIGACHHARLFFFF*IYF**RWGLAMM
VRLVSNS*PQVIRPPPRPPKVLGLQA*ATAPSQ
*LTLLERLLCARH*HFIQTSASQRF*PEKSRENE
GCLPGTGEHSLKSTNPRVKLFEVSCFIVYKQNK

Frame 1

VCVCV*ARARARVCTRACACVCLGVTSQLPEHK
DSQVRLPGSKLALQGT*EAHRRPTSWPSPSSAS
SQRCVPSCARHHVPAPGHLPDARWEYPWCWMAVA
AAGYVHGGWGSPATNSTSATPARAWSASPGQDP
VAGGPCASWQRTTAAVR*TAACIGKGRPSSPTA
ASAAAARTAASPACRCAARMCGCPAGTAPTPGGS
RSWASAALSGCAAKEGDWGPSPFQPKDPSFLAL
SLP
CPLVSPAQNGARPGDPARPPVGWAWPPGCP
TRTASADWRPSAACACPGPAHPPGVAVHKTVPSR
AGLGMGTRCPPSPAGGPVPGPWADGRWSVPRPL
AAGNTLAWVHHAEHQY*HAAWSVWIPRYGRGAR
PSPLSSNSLPRRLAKVSRVL*PTPCLHTQPISNM
HTGELSLRLPLGKRWDKQSLNIEAAAGAGLDWP
FFWG*DEEKAHRDSGSPAAFSGVCKIVPEYKPM
RDLVCQACVF*ESPPIIRGRIQGQSSL*TSQGEI
TWS*RKPEDRPSSDPRNPIHINNN*LFYLFIFL
RWSFALVAQAGAGVQ*HDLDSLQPPGFK*FSCL
KTPE*LGL*AHATMLGFFFFFKYIFSRDGV*P*W
SGWSRTPDLK*SARRLGLPKCWDYRRELLRPAN
N*HCLSACYVLGTSTLYRPVLPSVFDQRRAGRM
KAASQGLGSIA*NQLTQG*NCSKSHVLLFTNKTK

Frame 2

CVCVCERARARACVLVRVPVCAWE*PHSCRNIK
THRSASQAQSWLCRGHERHTEDPPPGLLPPLPP
LKGAYPAVPDTMYLPLATSPMPAGSTPGAGWLW
LLPGMCTAAGGALRPTPRLRRQPGPGLPARGRTR
WPGGPVPLGRGRQQL*GERPPVSGRGDLPAPLQ
HPLPLRGRRLHLRAAVQRGCAAAQLGLPPPQEG
RGPGQVLP*VGVRPRRGTGDPAPSSPRTPVFWPC
LF
PAPWCPLPRMEHGLGTLLDHLWAGHGHPGVQ
PEPLLPTGDPAPPVPVQALPTLQGSQSTKQCLL
EPGWEWGHGVHHPQLVALCLGPGLMEDGPCPGPW
LQATL*LGSTMQNTNINTLPGLSGSRGMAEVQD
LVPFPLTHCLGGWPRCPGSSSPLPAYTHSLYQT
CTRASFLSDFPWARDGTSSPLILRLQQVLGWTGH
FSGGRMKRRHTEILDLLLPFLEFVKLFLNTSLC
VILCVRPVFSEKALPLSGAEFRGRVLCEPHKVK
SHGLKESQKTGLQVIQETQFILIIINFFIYLFF*
DGVLLLLPRLELEYNSTISTHCSLPGSSDSPAS
RLLSSWDYRRMPPCSAFFFFLNIFLVEMGFSHD
GQAGLELLTSSDPPAASASQSAGITGVSYCAQPI
INIA*APAMC*ALALYTDQCFPAFLTREEQGE*
RLPPRDWGA*PEIN*PKGEIVRSLMFYCLQTKQ
N

Sequence WISP2.aAug05

AAACCGCAGGCTGGGCCGCGGCTACCCAGAGTCAGGGCCACGGAGCTTAGGAGACCTTGGGTCAGCTCTGCAAAGGGGTTGTTTACTGAATGCTTCTGGT
ATCTGCAGCCCCGCTGGGGTTCGGACAGGGGGTCTGGAATAGGGCTTCACAACTACAGCAGGGCTAGGACCCTCTTGGGCTGGAAGGAGCTGGAGAGGCC
TCTGCAAGCTGTCACAGGCTCTTGGTGCTGGCACTGGCTCAGGCTTTCACACACACACACGCGCACACACACACACACACACACACGGACAGGCACCCCC
TTGGTGGCCTTCACAGTTTCACCTTCAGGCTCAAAGCTGGCTCTGCAGGGGACATGAGAGGCACACCGAAGACCCACCTCCTGGCCTTCTCCCTCCTCTG
CCTCCTCTCAAAGGTGCGTACCCAGCTGTGCCCGACACCATGTACCTGCCCCTGGCCACCTCCCCGATGCCCGCTGGGAGTACCCCTGGTGCTGGATGGC
TGTGGCTGCTGCCGGGTATGTGCACGGCGGCTGGGGGAGCCCTGCGACCAACTCCACGTCTGCGACGCCAGCCAGGGCCTGGTCTGCCAGCCCGGGGCAG
GACCCGGTGGCCGGGGGGCCCTGTGCCTCTTGGCAGAGGACGACAGCAGCTGTGAGGTGAACGGCCGCCTGTATCGGGAAGGGGAGACCTTCCAGCCCCA
CTGCAGCATCCGCTGCCGCTGCGAGGACGGCGGCTTCACCTGCGTGCCGCTGTGCAGCGAGGATGTGCGGCTGCCCAGCTGGGACTGCCCCCACCCCAGG
AGGGTCGAGGTCCTGGGCAAGTGCTGCCCTGAGTGGGTGTGCGGCCAAGGAGGGGGACTGGGGACCCAGCCCCTTCCAGCCCAAGGACCCCAGTTTTCTG
GCCTTGTCTCTTCC
CTGCCCCCTGGTGTCCCCTGCCCAGAATGGAGCACGGCCTGGGGACCCTGCTCGACCACCTGTGGGCTGGGCATGGCCACCCGGGT
GTCCAACCAGAACCGCTTCTGCCGACTGGAGACCCAGCGCCGCCTGTGCCTGTCCAGGCCCTGCCCACCCTCCAGGGGTCGCAGTCCACAAAACAGTGCC
TTCTAGAGCCGGGCTGGGAATGGGGACACGGTGTCCACCATCCCCAGCTGGTGGCCCTGTGCCTGGGCCCTGGGCTGATGGAAGATGGTCCGTGCCCAGG
CCCTTGGCTGCAGGCAACACTTTAGCTTGGGTCCACCATGCAGAACACCAATATTAACACGCTGCCTGGTCTGTCTGGATCCCGAGGTATGGCAGAGGTG
CAAGACCTAGTCCCCTTTCCTCTAACTCACTGCCTAGGAGGCTGGCCAAGGTGTCCAGGGTCCTCTAGCCCACTCCCTGCCTACACACACAGCCTATATC
AAACATGCACACGGGCGAGCTTTCTCTCCGACTTCCCCTGGGCAAGAGATGGGACAAGCAGTCCCTTAATATTGAGGCTGCAGCAGGTGCTGGGCTGGAC
TGGCCATTTTTCTGGGGGTAGGATGAAGAGAAGGCACACAGAGATTCTGGATCTCCTGCTGCCTTTTCTGGAGTTTGTAAAATTGTTCCTGAATACAAGC
CTATGCGTGATCTTGTGTGTCAGGCCTGTGTTTTCTGAGAAAGCCCTCCCATTATCCGGGGCAGAATTCAGGGGCAGAGTTCTCTGTGAACCTCACAAGG
TGAAATCACATGGTCTTAAAGAAAGCCAGAAGACAGGCCTTCAAGTGATCCAAGAAACCCAATTCATATTAATAATAATTAACTTTTTTATTTATTTATT
TTTTTGAGATGGAGTTTTGCTCTTGTTGCCCAGGCTGGAGCTGGAGTACAATAGCACGATCTCGACTCACTGCAGCCTCCCGGGTTCAAGTGATTCTCCT
GCCTCAAGACTCCTGAGTAGCTGGGATTATAGGCGCATGCCACCATGCTCGGCTTTTTTTTTTTTTTTAAATATATTTTTAGTAGAGATGGGGTTTAGCC
ATGATGGTCAGGCTGGTCTCGAACTCCTGACCTCAAGTGATCCGCCCGCCGCCTCGGCCTCCCAAAGTGCTGGGATTACAGGCGTGAGCTACTGCGCCCA
GCCAATAATTAACATTGCTTGAGCGCCTGCTATGTGCTAGGCACTAGCACTTTATACAGACCAGTGCTTCCCAGCGTTTTTGACCAGAGAAGAGCAGGGA
GAATGAAGGCTGCCTCCCAGGGACTGGGGAGCATAGCCTGAAATCAACTAACCCAAGGGTGAAATTGTTCGAAGTCTCATGTTTTATTGTTTACAAACAA
AACAAAAC
  • WISP2.F1 (forward primer)
  • WISP2.R1 (reverse primer)
  • Alternative donor

Frame 0

KPQAGPRLPRVRATELRRPWVSSAKGLFTECFW
YLQPRWGSDRGSGIGLHNYSRARTLLGWKELERP
LQAVTGSWCWHWLRLSHTHTRTHTHTHTRTGTP
LVAFTVSPSGSKLALQGT*EAHRRPTSWPSPSS
ASSQRCVPSCARHHVPAPGHLPDARWEYPWCWMA
VAAAGYVHGGWGSPATNSTSATPARAWSASPGQ
DPVAGGPCASWQRTTAAVR*TAACIGKGRPSSP
TAASAAAARTAASPACRCAARMCGCPAGTAPTPG
GSRSWASAALSGCAAKEGDWGPSPFQPKDPSFL
ALSLP
CPLVSPAQNGARPGDPARPPVGWAWPPG
CPTRTASADWRPSAACACPGPAHPPGVAVHKTVP
SRAGLGMGTRCPPSPAGGPVPGPWADGRWSVPR
PLAAGNTLAWVHHAEHQY*HAAWSVWIPRYGRG
ARPSPLSSNSLPRRLAKVSRVL*PTPCLHTQPIS
NMHTGELSLRLPLGKRWDKQSLNIEAAAGAGLD
WPFFWG*DEEKAHRDSGSPAAFSGVCKIVPEYK
PMRDLVCQACVF*ESPPIIRGRIQGQSSL*TSQG
EITWS*RKPEDRPSSDPRNPIHINNN*LFYLFI
FLRWSFALVAQAGAGVQ*HDLDSLQPPGFK*FS
CLKTPE*LGL*AHATMLGFFFFFKYIFSRDGV*P
*WSGWSRTPDLK*SARRLGLPKCWDYRRELLRP
ANN*HCLSACYVLGTSTLYRPVLPSVFDQRRAG
RMKAASQGLGSIA*NQLTQG*NCSKSHVLLFTNK
TK

Frame 1

NRRLGRGYPESGPRSLGDLGSALQRGCLLNASG
ICSPAGVRTGGLE*GFTTTAGLGPSWAGRSWRG
LCKLSQALGAGTGSGFHTHTRAHTHTHTHGQAPP
WWPSQFHLQAQSWLCRGHERHTEDPPPGLLPPL
PPLKGAYPAVPDTMYLPLATSPMPAGSTPGAGW
LWLLPGMCTAAGGALRPTPRLRRQPGPGLPARGR
TRWPGGPVPLGRGRQQL*GERPPVSGRGDLPAP
LQHPLPLRGRRLHLRAAVQRGCAAAQLGLPPPQ
EGRGPGQVLP*VGVRPRRGTGDPAPSSPRTPVFW
PCLF
PAPWCPLPRMEHGLGTLLDHLWAGHGHPG
VQPEPLLPTGDPAPPVPVQALPTLQGSQSTKQC
LLEPGWEWGHGVHHPQLVALCLGPGLMEDGPCPG
PWLQATL*LGSTMQNTNINTLPGLSGSRGMAEV
QDLVPFPLTHCLGGWPRCPGSSSPLPAYTHSLY
QTCTRASFLSDFPWARDGTSSPLILRLQQVLGWT
GHFSGGRMKRRHTEILDLLLPFLEFVKLFLNTS
LCVILCVRPVFSEKALPLSGAEFRGRVLCEPHK
VKSHGLKESQKTGLQVIQETQFILIIINFFIYLF
F*DGVLLLLPRLELEYNSTISTHCSLPGSSDSP
ASRLLSSWDYRRMPPCSAFFFFLNIFLVEMGFS
HDGQAGLELLTSSDPPAASASQSAGITGVSYCAQ
PIINIA*APAMC*ALALYTDQCFPAFLTREEQG
E*RLPPRDWGA*PEIN*PKGEIVRSLMFYCLQT
KQN

Frame 2

TAGWAAATQSQGHGA*ETLGQLCKGVVY*MLLV
SAAPLGFGQGVWNRASQLQQG*DPLGLEGAGEA
SASCHRLLVLALAQAFTHTHAHTHTHTHTDRHP
LGGLHSFTFRLKAGSAGDMRGTPKTHLLAFSLLC
LLSKVRTQLCPTPCTCPWPPPRCPLGVPLVLDG
CGCCRVCARRLGEPCDQLHVCDASQGLVCQPGA
GPGGRGALCLLAEDDSSCEVNGRLYREGETFQPH
CSIRCRCEDGGFTCVPLCSEDVRLPSWDCPHPR
RVEVLGKCCPEWVCGQGGGLGTQPLPAQGPQFS
GLVSS
LPPGVPCPEWSTAWGPCSTTCGLGMATRV
SNQNRFCRLETQRRLCLSRPCPPSRGRSPQNSA
F*SRAGNGDTVSTIPSWWPCAWALG*WKMVRAQ
ALGCRQHFSLGPPCRTPILTRCLVCLDPEVWQRC
KT*SPFL*LTA*EAGQGVQGPLAHSLPTHTAYI
KHAHGRAFSPTSPGQEMGQAVP*Y*GCSRCWAG
LAIFLGVG*REGTQRFWISCCLFWSL*NCS*IQA
YA*SCVSGLCFLRKPSHYPGQNSGAEFSVNLTR
*NHMVLKKARRQAFK*SKKPNSY***LTFLFIY
FFEMEFCSCCPGWSWSTIARSRLTAASRVQVILL
PQDS*VAGIIGACHHARLFFFF*IYF**RWGLA
MMVRLVSNS*PQVIRPPPRPPKVLGLQA*ATAP
SQ*LTLLERLLCARH*HFIQTSASQRF*PEKSRE
NEGCLPGTGEHSLKSTNPRVKLFEVSCFIVYKQ
NK

Sequence WISP2.eAug05

GGCTGCTGCCGGGTATGTGCACGGCGGCTGGGGGAGCCCTGCGACCAACTCCACGTCTGCGACGCCAGCCAGGGCCTGGTCTGCCAGCCCGGGGCAGGAC
CCGGTGGCCGGGGGGCCCTGTGCCTCTGTAAGCAGTGGCAGAGGACGACAGCAGCTGTGAGGTGAACGGCCGCCTGTATCGGGAAGGGGAGACCTTCCAG
CCCCACTGCAGCATCCGCTGCCGCTGCGAGGACGGCGGCTTCACCTGCGTGCCGCTGTGCAGCGAGGATGTGCGGCTGCCCAGCTGGGACTGCCCCCACC
CCAGGAGGGTCGAGGTCCTGGGCAAGTGCTGCCCTGAGTGGGTGTGCGGCCAAGGAGGGGGACTGGGGACCCAGCCCCTTCCAGCCCAAGGACCCCAGTT
TTCTGGCCTTGTCTCTTCC
CTGCCCCCTGGTGTCCCCTGCCCAGAATGGAGCACGGCCTGGGGACCCTGCTCGACCACCTGTGGGCTGGGCATGGCCACC
CGGGTGTCCAACCAGAACCGCTTCTGCCGACTGGAGACCCAGCGCCGCCTGTGCCTGTCCAGGCCCTGCCCACCCTCCAGGGGTCGCAGTCCACAAAACA
GTGCCTTCTAGAGCCGGGCTGGGAATGGGGACACGGTGTCCACCATCCCCAGCTGGTGGCCCTGTGCCTGGGCCCTGGGCTGATGGAAGATGGTCCGTGC
CCAGGCCCTTGGCTGCAGGCAACACTTTAGCTTGGGTCCACCATGCAGAACACCAATATTAACACGCTGCCTGGTCTGTCTGGATCCCGAGGTATGGCAG
AGGTGCAAGACCTAGTCCCCTTTCCTCTAACTCACTGCCTAGGAGGCTGGCCAAGGTGTCCAGGGTCCTCTAGCCCACTCCCTGCCTACACACACAGCCT
ATATCAAACATGCACACGGGCGAGCTTTCTCTCCGACTTCCCCTGGGCAAGAGATGGGACAAGCAGTCCCTTAATATTGAGGCTGCAGCAGGTGCTGGGC
TGGACTGGCCATTTTTCTGGGGGTAGGATGAAGAGAAGGCACACAGAGATTCTGGATCTCCTGCTGCCTTTTCTGGAGTTTGTAAAATTGTTCCTGAATA
CAAGCCTATGCGTGATCTTGTGTGTCAGGCCTGTGTTTTCTGAGAAAGCCCTCCCATTATCCGGGGCAGAATTCAGGGGCAGAGTTCTCTGTGAACCTCA
CAAGGTGAAATCACATGGTCTTAAAGAAAGCCAGAAGACAGGCCTTCAAGTGATCCAAGAAACCCAATTCATATTAATAATAATTAACTTTTTTATTTAT
TTATTTTTTTGAGATGGAGTTTTGCTCTTGTTGCCCAGGCTGGAGCTGGAGTACAATAGCACGATCTCGACTCACTGCAGCCTCCCGGGTTCAAGTGATT
CTCCTGCCTCAAGACTCCTGAGTAGCTGGGATTATAGGCGCATGCCACCATGCTCGGCTTTTTTTTTTTTTTTAAATATATTTTTAGTAGAGATGGGGTT
TAGCCATGATGGTCAGGCTGGTCTCGAACTCCTGACCTCAAGTGATCCGCCCGCCGCCTCGGCCTCCCAAAGTGCTGGGATTACAGGCGTGAGCTACTGC
GCCCAGCCAATAATTAACATTGCTTGAGCGCCTGCTATGTGCTAGGCACTAGCACTTTATACAGACCAGTGCTTCCCAGCGTTTTTGACCAGAGAAGAGC
AGGGAGAATGAAGGCTGCCTCCCAGGGACTGGGGAGCATAGCCTGAAATCAACTAACCCAAGGGTGAAATTGTTCGAAGTCTCATGTTTTATTGTTTACA
AACAAAACAAAAC
  • WISP2.F1 (forward primer)
  • WISP2.R1 (reverse primer)
  • Alternative donor

Frame 0

GCCRVCARRLGEPCDQLHVCDASQGLVCQPGAG
PGGRGALCLCKQWQRTTAAVR*TAACIGKGRPSS
PTAASAAAARTAASPACRCAARMCGCPAGTAPT
PGGSRSWASAALSGCAAKEGDWGPSPFQPKDPS
FLALSLP
CPLVSPAQNGARPGDPARPPVGWAWPP
GCPTRTASADWRPSAACACPGPAHPPGVAVHKT
VPSRAGLGMGTRCPPSPAGGPVPGPWADGRWSV
PRPLAAGNTLAWVHHAEHQY*HAAWSVWIPRYGR
GARPSPLSSNSLPRRLAKVSRVL*PTPCLHTQP
ISNMHTGELSLRLPLGKRWDKQSLNIEAAAGAG
LDWPFFWG*DEEKAHRDSGSPAAFSGVCKIVPEY
KPMRDLVCQACVF*ESPPIIRGRIQGQSSL*TS
QGEITWS*RKPEDRPSSDPRNPIHINNN*LFYL
FIFLRWSFALVAQAGAGVQ*HDLDSLQPPGFK*F
SCLKTPE*LGL*AHATMLGFFFFFKYIFSRDGV
*P*WSGWSRTPDLK*SARRLGLPKCWDYRRELL
RPANN*HCLSACYVLGTSTLYRPVLPSVFDQRRA
GRMKAASQGLGSIA*NQLTQG*NCSKSHVLLFT
NKTK

Frame 1

AAAGYVHGGWGSPATNSTSATPARAWSASPGQD
PVAGGPCASVSSGRGRQQL*GERPPVSGRGDLP
APLQHPLPLRGRRLHLRAAVQRGCAAAQLGLPPP
QEGRGPGQVLP*VGVRPRRGTGDPAPSSPRTPV
FWPCLF
PAPWCPLPRMEHGLGTLLDHLWAGHGH
PGVQPEPLLPTGDPAPPVPVQALPTLQGSQSTKQ
CLLEPGWEWGHGVHHPQLVALCLGPGLMEDGPC
PGPWLQATL*LGSTMQNTNINTLPGLSGSRGMA
EVQDLVPFPLTHCLGGWPRCPGSSSPLPAYTHSL
YQTCTRASFLSDFPWARDGTSSPLILRLQQVLG
WTGHFSGGRMKRRHTEILDLLLPFLEFVKLFLN
TSLCVILCVRPVFSEKALPLSGAEFRGRVLCEPH
KVKSHGLKESQKTGLQVIQETQFILIIINFFIY
LFF*DGVLLLLPRLELEYNSTISTHCSLPGSSD
SPASRLLSSWDYRRMPPCSAFFFFLNIFLVEMGF
SHDGQAGLELLTSSDPPAASASQSAGITGVSYC
AQPIINIA*APAMC*ALALYTDQCFPAFLTREE
QGE*RLPPRDWGA*PEIN*PKGEIVRSLMFYCLQ
TKQN

Frame 2

LLPGMCTAAGGALRPTPRLRRQPGPGLPARGRT
RWPGGPVPL*AVAEDDSSCEVNGRLYREGETFQ
PHCSIRCRCEDGGFTCVPLCSEDVRLPSWDCPH
PRRVEVLGKCCPEWVCGQGGGLGTQPLPAQGPQF
SGLVSS
LPPGVPCPEWSTAWGPCSTTCGLGMAT
RVSNQNRFCRLETQRRLCLSRPCPPSRGRSPQN
SAF*SRAGNGDTVSTIPSWWPCAWALG*WKMVRA
QALGCRQHFSLGPPCRTPILTRCLVCLDPEVWQ
RCKT*SPFL*LTA*EAGQGVQGPLAHSLPTHTA
YIKHAHGRAFSPTSPGQEMGQAVP*Y*GCSRCWA
GLAIFLGVG*REGTQRFWISCCLFWSL*NCS*I
QAYA*SCVSGLCFLRKPSHYPGQNSGAEFSVNL
TR*NHMVLKKARRQAFK*SKKPNSY***LTFLFI
YFFEMEFCSCCPGWSWSTIARSRLTAASRVQVI
LLPQDS*VAGIIGACHHARLFFFF*IYF**RWG
LAMMVRLVSNS*PQVIRPPPRPPKVLGLQA*ATA
PSQ*LTLLERLLCARH*HFIQTSASQRF*PEKS
RENEGCLPGTGEHSLKSTNPRVKLFEVSCFIVY
KQNK

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