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Sequences

Sequence WISP2.dAug05

GGAGACCTTGGGTCAGCTCTGCAAAGGGGTTGTTTACTGAATGCTTCTGGTATCTGCAGCCCCGCTGGGGTTCGGACAGGGGGTCTGGAATAGGGCTTCA CAACTACAGCAGGGCTAGGACCCTCTTGGGCTGGAAGGAGCTGGAGAGGCCTCTGCAAGCTGTCACAGGCTCTTGGTGCTGGCACTGGCTCAGGCTTTCA CACACACACACGCGCACACACACACACACACACACACGGACAGGCACCCCCTTGGTGGCCTTCACAGTTTCACCTTCAGGTAAATGGGCTCATCCTTTGA GCCATGAGGATGGGAAGCGAAGCAAGGAATGAAAAAGCTAGTGTGTTTGTGTGTGTGTGTGTGTGTGAGCGCGCGCGCGCGCGCGCGTGTGTACTCGTGC GTGTGCCTGTGTGTGCCTGGGAGTGACCTCACAGCTGCCGGAACATAAAGACTCACAGGTCCGCCTCCCAGGCTCAAAGCTGGCTCTGCAGGGGACATGA GAGGCACACCGAAGACCCACCTCCTGGCCTTCTCCCTCCTCTGCCTCCTCTCAAAGGTGCGTACCCAGCTGTGCCCGACACCATGTACCTGCCCCTGGCC ACCTCCCCGATGCCCGCTGGGAGTACCCCTGGTGCTGGATGGCTGTGGCTGCTGCCGGGTATGTGCACGGCGGCTGGGGGAGCCCTGCGACCAACTCCAC GTCTGCGACGCCAGCCAGGGCCTGGTCTGCCAGCCCGGGGCAGGACCCGGTGGCCGGGGGGCCCTGTGCCTCTGTAAGCAGGACCCCAGTTTTCTGGCCT TGTCTCTTCCCTGCCCCCTGGTGTCCCCTGCCCAGAATGGAGCACGGCCTGGGGACCCTGCTCGACCACCTGTGGGCTGGGCATGGCCACCCGGGTGTCC AACCAGAACCGCTTCTGCCGACTGGAGACCCAGCGCCGCCTGTGCCTGTCCAGGCCCTGCCCACCCTCCAGGGGTCGCAGTCCACAAAACAGTGCCTTCT AGAGCCGGGCTGGGAATGGGGACACGGTGTCCACCATCCCCAGCTGGTGGCCCTGTGCCTGGGCCCTGGGCTGATGGAAGATGGTCCGTGCCCAGGCCCT TGGCTGCAGGCAACACTTTAGCTTGGGTCCACCATGCAGAACACCAATATTAACACGCTGCCTGGTCTGTCTGGATCCCGAGGTATGGCAGAGGTGCAAG ACCTAGTCCCCTTTCCTCTAACTCACTGCCTAGGAGGCTGGCCAAGGTGTCCAGGGTCCTCTAGCCCACTCCCTGCCTACACACACAGCCTATATCAAAC ATGCACACGGGCGAGCTTTCTCTCCGACTTCCCCTGGGCAAGAGATGGGACAAGCAGTCCCTTAATATTGAGGCTGCAGCAGGTGCTGGGCTGGACTGGC CATTTTTCTGGGGGTAGGATGAAGAGAAGGCACACAGAGATTCTGGATCTCCTGCTGCCTTTTCTGGAGTTTGTAAAATTGTTCCTGAATACAAGCCTAT GCGTGATCTTGTGTGTCAGGCCTGTGTTTTCTGAGAAAGCCCTCCCATTATCCGGGGCAGAATTCAGGGGCAGAGTTCTCTGTGAACCTCACAAGGTGAA ATCACATGGTCTTAAAGAAAGCCAGAAGACAGGCCTTCAAGTGATCCAAGAAACCCAATTCATATTAATAATAATTAACTTTTTTATTTATTTATTTTTT TGAGATGGAGTTTTGCTCTTGTTGCCCAGGCTGGAGCTGGAGTACAATAGCACGATCTCGACTCACTGCAGCCTCCCGGGTTCAAGTGATTCTCCTGCCT CAAGACTCCTGAGTAGCTGGGATTATAGGCGCATGCCACCATGCTCGGCTTTTTTTTTTTTTTTAAATATATTTTTAGTAGAGATGGGGTTTAGCCATGA TGGTCAGGCTGGTCTCGAACTCCTGACCTCAAGTGATCCGCCCGCCGCCTCGGCCTCCCAAAGTGCTGGGATTACAGGCGTGAGCTACTGCGCCCAGCCA ATAATTAACATTGCTTGAGCGCCTGCTATGTGCTAGGCACTAGCACTTTATACAGACCAGTGCTTCCCAGCGTTTTTGACCAGAGAAGAGCAGGGAGAAT GAAGGCTGCCTCCCAGGGACTGGGGAGCATAGCCTGAAATCAACTAACCCAAGGGTGAAATTGTTCGAAGTCTCATGTTTTATTGTTTACAAACAAAACA AAAC

WISP2.F1 (forward primer)
WISP2.R1 (reverse primer)
Exon skipping and alternative donor or acceptor

Frame 0

GDLGSALQRGCLLNASGICSPAGVRTGGLE*GF TTTAGLGPSWAGRSWRGLCKLSQALGAGTGSGFH THTRAHTHTHTHGQAPPWWPSQFHLQVNGLIL* AMRMGSEARNEKASVFVCVCVCERARARACVLV RVPVCAWE*PHSCRNIKTHRSASQAQSWLCRGHE RHTEDPPPGLLPPLPPLKGAYPAVPDTMYLPLA TSPMPAGSTPGAGWLWLLPGMCTAAGGALRPTP RLRRQPGPGLPARGRTRWPGGPVPL*AGPQFSGL VSSLPPGVPCPEWSTAWGPCSTTCGLGMATRVS NQNRFCRLETQRRLCLSRPCPPSRGRSPQNSAF *SRAGNGDTVSTIPSWWPCAWALG*WKMVRAQAL GCRQHFSLGPPCRTPILTRCLVCLDPEVWQRCK T*SPFL*LTA*EAGQGVQGPLAHSLPTHTAYIK HAHGRAFSPTSPGQEMGQAVP*Y*GCSRCWAGLA IFLGVG*REGTQRFWISCCLFWSL*NCS*IQAY A*SCVSGLCFLRKPSHYPGQNSGAEFSVNLTR* NHMVLKKARRQAFK*SKKPNSY***LTFLFIYFF EMEFCSCCPGWSWSTIARSRLTAASRVQVILLP QDS*VAGIIGACHHARLFFFF*IYF**RWGLAM MVRLVSNS*PQVIRPPPRPPKVLGLQA*ATAPSQ *LTLLERLLCARH*HFIQTSASQRF*PEKSREN EGCLPGTGEHSLKSTNPRVKLFEVSCFIVYKQN K

Frame 1

ETLGQLCKGVVY*MLLVSAAPLGFGQGVWNRAS QLQQG*DPLGLEGAGEASASCHRLLVLALAQAF THTHAHTHTHTHTDRHPLGGLHSFTFR*MGSSFE P*GWEAKQGMKKLVCLCVCVCVSARARARVYSC VCLCVPGSDLTAAGT*RLTGPPPRLKAGSAGDM RGTPKTHLLAFSLLCLLSKVRTQLCPTPCTCPWP PPRCPLGVPLVLDGCGCCRVCARRLGEPCDQLH VCDASQGLVCQPGAGPGGRGALCLCKQDPSFLA LSLPCPLVSPAQNGARPGDPARPPVGWAWPPGCP TRTASADWRPSAACACPGPAHPPGVAVHKTVPS RAGLGMGTRCPPSPAGGPVPGPWADGRWSVPRP LAAGNTLAWVHHAEHQY*HAAWSVWIPRYGRGAR PSPLSSNSLPRRLAKVSRVL*PTPCLHTQPISN MHTGELSLRLPLGKRWDKQSLNIEAAAGAGLDW PFFWG*DEEKAHRDSGSPAAFSGVCKIVPEYKPM RDLVCQACVF*ESPPIIRGRIQGQSSL*TSQGE ITWS*RKPEDRPSSDPRNPIHINNN*LFYLFIF LRWSFALVAQAGAGVQ*HDLDSLQPPGFK*FSCL KTPE*LGL*AHATMLGFFFFFKYIFSRDGV*P* WSGWSRTPDLK*SARRLGLPKCWDYRRELLRPA NN*HCLSACYVLGTSTLYRPVLPSVFDQRRAGRM KAASQGLGSIA*NQLTQG*NCSKSHVLLFTNKT K

Frame 2

RPWVSSAKGLFTECFWYLQPRWGSDRGSGIGLH NYSRARTLLGWKELERPLQAVTGSWCWHWLRLS HTHTRTHTHTHTRTGTPLVAFTVSPSGKWAHPL SHEDGKRSKE*KS*CVCVCVCV*ARARARVCTRA CACVCLGVTSQLPEHKDSQVRLPGSKLALQGT* EAHRRPTSWPSPSSASSQRCVPSCARHHVPAPG HLPDARWEYPWCWMAVAAAGYVHGGWGSPATNST SATPARAWSASPGQDPVAGGPCASVSRTPVFWP CLFPAPWCPLPRMEHGLGTLLDHLWAGHGHPGV QPEPLLPTGDPAPPVPVQALPTLQGSQSTKQCLL EPGWEWGHGVHHPQLVALCLGPGLMEDGPCPGP WLQATL*LGSTMQNTNINTLPGLSGSRGMAEVQ DLVPFPLTHCLGGWPRCPGSSSPLPAYTHSLYQT CTRASFLSDFPWARDGTSSPLILRLQQVLGWTG HFSGGRMKRRHTEILDLLLPFLEFVKLFLNTSL CVILCVRPVFSEKALPLSGAEFRGRVLCEPHKVK SHGLKESQKTGLQVIQETQFILIIINFFIYLFF *DGVLLLLPRLELEYNSTISTHCSLPGSSDSPA SRLLSSWDYRRMPPCSAFFFFLNIFLVEMGFSHD GQAGLELLTSSDPPAASASQSAGITGVSYCAQP IINIA*APAMC*ALALYTDQCFPAFLTREEQGE *RLPPRDWGA*PEIN*PKGEIVRSLMFYCLQTKQ N

Sequence WISP2.bAug05

TGTGTGTGTGTGTGTGTGAGCGCGCGCGCGCGCGCGCGTGTGTACTCGTGCGTGTGCCTGTGTGTGCCTGGGAGTGACCTCACAGCTGCCGGAACATAAA GACTCACAGGTCCGCCTCCCAGGCTCAAAGCTGGCTCTGCAGGGGACATGAGAGGCACACCGAAGACCCACCTCCTGGCCTTCTCCCTCCTCTGCCTCCT CTCAAAGGTGCGTACCCAGCTGTGCCCGACACCATGTACCTGCCCCTGGCCACCTCCCCGATGCCCGCTGGGAGTACCCCTGGTGCTGGATGGCTGTGGC TGCTGCCGGGTATGTGCACGGCGGCTGGGGGAGCCCTGCGACCAACTCCACGTCTGCGACGCCAGCCAGGGCCTGGTCTGCCAGCCCGGGGCAGGACCCG GTGGCCGGGGGGCCCTGTGCCTCTTGGCAGAGGACGACAGCAGCTGTGAGGTGAACGGCCGCCTGTATCGGGAAGGGGAGACCTTCCAGCCCCACTGCAG CATCCGCTGCCGCTGCGAGGACGGCGGCTTCACCTGCGTGCCGCTGTGCAGCGAGGATGTGCGGCTGCCCAGCTGGGACTGCCCCCACCCCAGGAGGGTC GAGGTCCTGGGCAAGTGCTGCCCTGAGTGGGTGTGCGGCCAAGGAGGGGGACTGGGGACCCAGCCCCTTCCAGCCCAAGGACCCCAGTTTTCTGGCCTTG TCTCTTCCCTGCCCCCTGGTGTCCCCTGCCCAGAATGGAGCACGGCCTGGGGACCCTGCTCGACCACCTGTGGGCTGGGCATGGCCACCCGGGTGTCCAA CCAGAACCGCTTCTGCCGACTGGAGACCCAGCGCCGCCTGTGCCTGTCCAGGCCCTGCCCACCCTCCAGGGGTCGCAGTCCACAAAACAGTGCCTTCTAG AGCCGGGCTGGGAATGGGGACACGGTGTCCACCATCCCCAGCTGGTGGCCCTGTGCCTGGGCCCTGGGCTGATGGAAGATGGTCCGTGCCCAGGCCCTTG GCTGCAGGCAACACTTTAGCTTGGGTCCACCATGCAGAACACCAATATTAACACGCTGCCTGGTCTGTCTGGATCCCGAGGTATGGCAGAGGTGCAAGAC CTAGTCCCCTTTCCTCTAACTCACTGCCTAGGAGGCTGGCCAAGGTGTCCAGGGTCCTCTAGCCCACTCCCTGCCTACACACACAGCCTATATCAAACAT GCACACGGGCGAGCTTTCTCTCCGACTTCCCCTGGGCAAGAGATGGGACAAGCAGTCCCTTAATATTGAGGCTGCAGCAGGTGCTGGGCTGGACTGGCCA TTTTTCTGGGGGTAGGATGAAGAGAAGGCACACAGAGATTCTGGATCTCCTGCTGCCTTTTCTGGAGTTTGTAAAATTGTTCCTGAATACAAGCCTATGC GTGATCTTGTGTGTCAGGCCTGTGTTTTCTGAGAAAGCCCTCCCATTATCCGGGGCAGAATTCAGGGGCAGAGTTCTCTGTGAACCTCACAAGGTGAAAT CACATGGTCTTAAAGAAAGCCAGAAGACAGGCCTTCAAGTGATCCAAGAAACCCAATTCATATTAATAATAATTAACTTTTTTATTTATTTATTTTTTTG AGATGGAGTTTTGCTCTTGTTGCCCAGGCTGGAGCTGGAGTACAATAGCACGATCTCGACTCACTGCAGCCTCCCGGGTTCAAGTGATTCTCCTGCCTCA AGACTCCTGAGTAGCTGGGATTATAGGCGCATGCCACCATGCTCGGCTTTTTTTTTTTTTTTAAATATATTTTTAGTAGAGATGGGGTTTAGCCATGATG GTCAGGCTGGTCTCGAACTCCTGACCTCAAGTGATCCGCCCGCCGCCTCGGCCTCCCAAAGTGCTGGGATTACAGGCGTGAGCTACTGCGCCCAGCCAAT AATTAACATTGCTTGAGCGCCTGCTATGTGCTAGGCACTAGCACTTTATACAGACCAGTGCTTCCCAGCGTTTTTGACCAGAGAAGAGCAGGGAGAATGA AGGCTGCCTCCCAGGGACTGGGGAGCATAGCCTGAAATCAACTAACCCAAGGGTGAAATTGTTCGAAGTCTCATGTTTTATTGTTTACAAACAAAACAAA AC

WISP2.F1 (forward primer)
WISP2.R1 (reverse primer)
Exon skipping and alternative donor or acceptor

Frame 0

CVCVCVSARARARVYSCVCLCVPGSDLTAAGT* RLTGPPPRLKAGSAGDMRGTPKTHLLAFSLLCLL SKVRTQLCPTPCTCPWPPPRCPLGVPLVLDGCG CCRVCARRLGEPCDQLHVCDASQGLVCQPGAGP GGRGALCLLAEDDSSCEVNGRLYREGETFQPHCS IRCRCEDGGFTCVPLCSEDVRLPSWDCPHPRRV EVLGKCCPEWVCGQGGGLGTQPLPAQGPQFSGL VSSLPPGVPCPEWSTAWGPCSTTCGLGMATRVSN QNRFCRLETQRRLCLSRPCPPSRGRSPQNSAF* SRAGNGDTVSTIPSWWPCAWALG*WKMVRAQAL GCRQHFSLGPPCRTPILTRCLVCLDPEVWQRCKT *SPFL*LTA*EAGQGVQGPLAHSLPTHTAYIKH AHGRAFSPTSPGQEMGQAVP*Y*GCSRCWAGLA IFLGVG*REGTQRFWISCCLFWSL*NCS*IQAYA *SCVSGLCFLRKPSHYPGQNSGAEFSVNLTR*N HMVLKKARRQAFK*SKKPNSY***LTFLFIYFF EMEFCSCCPGWSWSTIARSRLTAASRVQVILLPQ DS*VAGIIGACHHARLFFFF*IYF**RWGLAMM VRLVSNS*PQVIRPPPRPPKVLGLQA*ATAPSQ *LTLLERLLCARH*HFIQTSASQRF*PEKSRENE GCLPGTGEHSLKSTNPRVKLFEVSCFIVYKQNK

Frame 1

VCVCV*ARARARVCTRACACVCLGVTSQLPEHK DSQVRLPGSKLALQGT*EAHRRPTSWPSPSSAS SQRCVPSCARHHVPAPGHLPDARWEYPWCWMAVA AAGYVHGGWGSPATNSTSATPARAWSASPGQDP VAGGPCASWQRTTAAVR*TAACIGKGRPSSPTA ASAAAARTAASPACRCAARMCGCPAGTAPTPGGS RSWASAALSGCAAKEGDWGPSPFQPKDPSFLAL SLPCPLVSPAQNGARPGDPARPPVGWAWPPGCP TRTASADWRPSAACACPGPAHPPGVAVHKTVPSR AGLGMGTRCPPSPAGGPVPGPWADGRWSVPRPL AAGNTLAWVHHAEHQY*HAAWSVWIPRYGRGAR PSPLSSNSLPRRLAKVSRVL*PTPCLHTQPISNM HTGELSLRLPLGKRWDKQSLNIEAAAGAGLDWP FFWG*DEEKAHRDSGSPAAFSGVCKIVPEYKPM RDLVCQACVF*ESPPIIRGRIQGQSSL*TSQGEI TWS*RKPEDRPSSDPRNPIHINNN*LFYLFIFL RWSFALVAQAGAGVQ*HDLDSLQPPGFK*FSCL KTPE*LGL*AHATMLGFFFFFKYIFSRDGV*P*W SGWSRTPDLK*SARRLGLPKCWDYRRELLRPAN N*HCLSACYVLGTSTLYRPVLPSVFDQRRAGRM KAASQGLGSIA*NQLTQG*NCSKSHVLLFTNKTK

Frame 2

CVCVCERARARACVLVRVPVCAWE*PHSCRNIK THRSASQAQSWLCRGHERHTEDPPPGLLPPLPP LKGAYPAVPDTMYLPLATSPMPAGSTPGAGWLW LLPGMCTAAGGALRPTPRLRRQPGPGLPARGRTR WPGGPVPLGRGRQQL*GERPPVSGRGDLPAPLQ HPLPLRGRRLHLRAAVQRGCAAAQLGLPPPQEG RGPGQVLP*VGVRPRRGTGDPAPSSPRTPVFWPC LFPAPWCPLPRMEHGLGTLLDHLWAGHGHPGVQ PEPLLPTGDPAPPVPVQALPTLQGSQSTKQCLL EPGWEWGHGVHHPQLVALCLGPGLMEDGPCPGPW LQATL*LGSTMQNTNINTLPGLSGSRGMAEVQD LVPFPLTHCLGGWPRCPGSSSPLPAYTHSLYQT CTRASFLSDFPWARDGTSSPLILRLQQVLGWTGH FSGGRMKRRHTEILDLLLPFLEFVKLFLNTSLC VILCVRPVFSEKALPLSGAEFRGRVLCEPHKVK SHGLKESQKTGLQVIQETQFILIIINFFIYLFF* DGVLLLLPRLELEYNSTISTHCSLPGSSDSPAS RLLSSWDYRRMPPCSAFFFFLNIFLVEMGFSHD GQAGLELLTSSDPPAASASQSAGITGVSYCAQPI INIA*APAMC*ALALYTDQCFPAFLTREEQGE* RLPPRDWGA*PEIN*PKGEIVRSLMFYCLQTKQ N

Sequence WISP2.aAug05

AAACCGCAGGCTGGGCCGCGGCTACCCAGAGTCAGGGCCACGGAGCTTAGGAGACCTTGGGTCAGCTCTGCAAAGGGGTTGTTTACTGAATGCTTCTGGT ATCTGCAGCCCCGCTGGGGTTCGGACAGGGGGTCTGGAATAGGGCTTCACAACTACAGCAGGGCTAGGACCCTCTTGGGCTGGAAGGAGCTGGAGAGGCC TCTGCAAGCTGTCACAGGCTCTTGGTGCTGGCACTGGCTCAGGCTTTCACACACACACACGCGCACACACACACACACACACACACGGACAGGCACCCCC TTGGTGGCCTTCACAGTTTCACCTTCAGGCTCAAAGCTGGCTCTGCAGGGGACATGAGAGGCACACCGAAGACCCACCTCCTGGCCTTCTCCCTCCTCTG CCTCCTCTCAAAGGTGCGTACCCAGCTGTGCCCGACACCATGTACCTGCCCCTGGCCACCTCCCCGATGCCCGCTGGGAGTACCCCTGGTGCTGGATGGC TGTGGCTGCTGCCGGGTATGTGCACGGCGGCTGGGGGAGCCCTGCGACCAACTCCACGTCTGCGACGCCAGCCAGGGCCTGGTCTGCCAGCCCGGGGCAG GACCCGGTGGCCGGGGGGCCCTGTGCCTCTTGGCAGAGGACGACAGCAGCTGTGAGGTGAACGGCCGCCTGTATCGGGAAGGGGAGACCTTCCAGCCCCA CTGCAGCATCCGCTGCCGCTGCGAGGACGGCGGCTTCACCTGCGTGCCGCTGTGCAGCGAGGATGTGCGGCTGCCCAGCTGGGACTGCCCCCACCCCAGG AGGGTCGAGGTCCTGGGCAAGTGCTGCCCTGAGTGGGTGTGCGGCCAAGGAGGGGGACTGGGGACCCAGCCCCTTCCAGCCCAAGGACCCCAGTTTTCTG GCCTTGTCTCTTCCCTGCCCCCTGGTGTCCCCTGCCCAGAATGGAGCACGGCCTGGGGACCCTGCTCGACCACCTGTGGGCTGGGCATGGCCACCCGGGT GTCCAACCAGAACCGCTTCTGCCGACTGGAGACCCAGCGCCGCCTGTGCCTGTCCAGGCCCTGCCCACCCTCCAGGGGTCGCAGTCCACAAAACAGTGCC TTCTAGAGCCGGGCTGGGAATGGGGACACGGTGTCCACCATCCCCAGCTGGTGGCCCTGTGCCTGGGCCCTGGGCTGATGGAAGATGGTCCGTGCCCAGG CCCTTGGCTGCAGGCAACACTTTAGCTTGGGTCCACCATGCAGAACACCAATATTAACACGCTGCCTGGTCTGTCTGGATCCCGAGGTATGGCAGAGGTG CAAGACCTAGTCCCCTTTCCTCTAACTCACTGCCTAGGAGGCTGGCCAAGGTGTCCAGGGTCCTCTAGCCCACTCCCTGCCTACACACACAGCCTATATC AAACATGCACACGGGCGAGCTTTCTCTCCGACTTCCCCTGGGCAAGAGATGGGACAAGCAGTCCCTTAATATTGAGGCTGCAGCAGGTGCTGGGCTGGAC TGGCCATTTTTCTGGGGGTAGGATGAAGAGAAGGCACACAGAGATTCTGGATCTCCTGCTGCCTTTTCTGGAGTTTGTAAAATTGTTCCTGAATACAAGC CTATGCGTGATCTTGTGTGTCAGGCCTGTGTTTTCTGAGAAAGCCCTCCCATTATCCGGGGCAGAATTCAGGGGCAGAGTTCTCTGTGAACCTCACAAGG TGAAATCACATGGTCTTAAAGAAAGCCAGAAGACAGGCCTTCAAGTGATCCAAGAAACCCAATTCATATTAATAATAATTAACTTTTTTATTTATTTATT TTTTTGAGATGGAGTTTTGCTCTTGTTGCCCAGGCTGGAGCTGGAGTACAATAGCACGATCTCGACTCACTGCAGCCTCCCGGGTTCAAGTGATTCTCCT GCCTCAAGACTCCTGAGTAGCTGGGATTATAGGCGCATGCCACCATGCTCGGCTTTTTTTTTTTTTTTAAATATATTTTTAGTAGAGATGGGGTTTAGCC ATGATGGTCAGGCTGGTCTCGAACTCCTGACCTCAAGTGATCCGCCCGCCGCCTCGGCCTCCCAAAGTGCTGGGATTACAGGCGTGAGCTACTGCGCCCA GCCAATAATTAACATTGCTTGAGCGCCTGCTATGTGCTAGGCACTAGCACTTTATACAGACCAGTGCTTCCCAGCGTTTTTGACCAGAGAAGAGCAGGGA GAATGAAGGCTGCCTCCCAGGGACTGGGGAGCATAGCCTGAAATCAACTAACCCAAGGGTGAAATTGTTCGAAGTCTCATGTTTTATTGTTTACAAACAA AACAAAAC

WISP2.F1 (forward primer)
WISP2.R1 (reverse primer)
Exon skipping and alternative donor or acceptor

Frame 0

KPQAGPRLPRVRATELRRPWVSSAKGLFTECFW YLQPRWGSDRGSGIGLHNYSRARTLLGWKELERP LQAVTGSWCWHWLRLSHTHTRTHTHTHTRTGTP LVAFTVSPSGSKLALQGT*EAHRRPTSWPSPSS ASSQRCVPSCARHHVPAPGHLPDARWEYPWCWMA VAAAGYVHGGWGSPATNSTSATPARAWSASPGQ DPVAGGPCASWQRTTAAVR*TAACIGKGRPSSP TAASAAAARTAASPACRCAARMCGCPAGTAPTPG GSRSWASAALSGCAAKEGDWGPSPFQPKDPSFL ALSLPCPLVSPAQNGARPGDPARPPVGWAWPPG CPTRTASADWRPSAACACPGPAHPPGVAVHKTVP SRAGLGMGTRCPPSPAGGPVPGPWADGRWSVPR PLAAGNTLAWVHHAEHQY*HAAWSVWIPRYGRG ARPSPLSSNSLPRRLAKVSRVL*PTPCLHTQPIS NMHTGELSLRLPLGKRWDKQSLNIEAAAGAGLD WPFFWG*DEEKAHRDSGSPAAFSGVCKIVPEYK PMRDLVCQACVF*ESPPIIRGRIQGQSSL*TSQG EITWS*RKPEDRPSSDPRNPIHINNN*LFYLFI FLRWSFALVAQAGAGVQ*HDLDSLQPPGFK*FS CLKTPE*LGL*AHATMLGFFFFFKYIFSRDGV*P *WSGWSRTPDLK*SARRLGLPKCWDYRRELLRP ANN*HCLSACYVLGTSTLYRPVLPSVFDQRRAG RMKAASQGLGSIA*NQLTQG*NCSKSHVLLFTNK TK

Frame 1

NRRLGRGYPESGPRSLGDLGSALQRGCLLNASG ICSPAGVRTGGLE*GFTTTAGLGPSWAGRSWRG LCKLSQALGAGTGSGFHTHTRAHTHTHTHGQAPP WWPSQFHLQAQSWLCRGHERHTEDPPPGLLPPL PPLKGAYPAVPDTMYLPLATSPMPAGSTPGAGW LWLLPGMCTAAGGALRPTPRLRRQPGPGLPARGR TRWPGGPVPLGRGRQQL*GERPPVSGRGDLPAP LQHPLPLRGRRLHLRAAVQRGCAAAQLGLPPPQ EGRGPGQVLP*VGVRPRRGTGDPAPSSPRTPVFW PCLFPAPWCPLPRMEHGLGTLLDHLWAGHGHPG VQPEPLLPTGDPAPPVPVQALPTLQGSQSTKQC LLEPGWEWGHGVHHPQLVALCLGPGLMEDGPCPG PWLQATL*LGSTMQNTNINTLPGLSGSRGMAEV QDLVPFPLTHCLGGWPRCPGSSSPLPAYTHSLY QTCTRASFLSDFPWARDGTSSPLILRLQQVLGWT GHFSGGRMKRRHTEILDLLLPFLEFVKLFLNTS LCVILCVRPVFSEKALPLSGAEFRGRVLCEPHK VKSHGLKESQKTGLQVIQETQFILIIINFFIYLF F*DGVLLLLPRLELEYNSTISTHCSLPGSSDSP ASRLLSSWDYRRMPPCSAFFFFLNIFLVEMGFS HDGQAGLELLTSSDPPAASASQSAGITGVSYCAQ PIINIA*APAMC*ALALYTDQCFPAFLTREEQG E*RLPPRDWGA*PEIN*PKGEIVRSLMFYCLQT KQN

Frame 2

TAGWAAATQSQGHGA*ETLGQLCKGVVY*MLLV SAAPLGFGQGVWNRASQLQQG*DPLGLEGAGEA SASCHRLLVLALAQAFTHTHAHTHTHTHTDRHP LGGLHSFTFRLKAGSAGDMRGTPKTHLLAFSLLC LLSKVRTQLCPTPCTCPWPPPRCPLGVPLVLDG CGCCRVCARRLGEPCDQLHVCDASQGLVCQPGA GPGGRGALCLLAEDDSSCEVNGRLYREGETFQPH CSIRCRCEDGGFTCVPLCSEDVRLPSWDCPHPR RVEVLGKCCPEWVCGQGGGLGTQPLPAQGPQFS GLVSSLPPGVPCPEWSTAWGPCSTTCGLGMATRV SNQNRFCRLETQRRLCLSRPCPPSRGRSPQNSA F*SRAGNGDTVSTIPSWWPCAWALG*WKMVRAQ ALGCRQHFSLGPPCRTPILTRCLVCLDPEVWQRC KT*SPFL*LTA*EAGQGVQGPLAHSLPTHTAYI KHAHGRAFSPTSPGQEMGQAVP*Y*GCSRCWAG LAIFLGVG*REGTQRFWISCCLFWSL*NCS*IQA YA*SCVSGLCFLRKPSHYPGQNSGAEFSVNLTR *NHMVLKKARRQAFK*SKKPNSY***LTFLFIY FFEMEFCSCCPGWSWSTIARSRLTAASRVQVILL PQDS*VAGIIGACHHARLFFFF*IYF**RWGLA MMVRLVSNS*PQVIRPPPRPPKVLGLQA*ATAP SQ*LTLLERLLCARH*HFIQTSASQRF*PEKSRE NEGCLPGTGEHSLKSTNPRVKLFEVSCFIVYKQ NK