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Sequences

Sequence PTK6.bAug05

TGCCACGCCCAGCTCTGGGTGCTCCAGCTGGGCCACAGCCTGGTCCTGCCGCTGCGCCCGCCCGCCATGGTGTCCCGGGACCAGGCTCACCTGGGCCCCA
AGTATGTGGGCCTCTGGGACTTCAAGTCCCGGACGGACGAGGAGCTGAGCTTCCGCGCGGGGGACGTCTTCCACGTGGCCAGGAAGGAGGAGCAGTGGTG
GTGGGCCACGCTGCTGGACGAGGCGGGTGGGGCCGTGGCCCAGGGCTATGTGCCCCACAACTACCTGGCCGAGAGGGAGACGGTGGAGTCGGAACCGTGG
TTCTTTGGCTGCATCT
CCCGCTCGGAAGCTGTGCGTCGGCTGCAGGCCGAGGGCAACGCCACGGGCGCCTTCCTGATCAGGTGCGGGACACGCAGGCTGT
GCGGCACTACAAGATCTGGCGGCGTGCCGGGGGCCGGCTGCACCTGAACGAGGCGGTGTCCTTCCTCAGCCTGCCCGAGCTTGTGAACTACCACAGGGCC
CAGAGCCTGTCCCACGGCCTGCGGCTGGCCGCGCCCTGCCGGAAGCACGAGCCTGAGCCCCTGCCCCATTGGGATGACTGGGAGAGGCCGAGGGAGGAGT
TCACGCTCTGCAGGAAGCTGGGGTCCGGCTACTTTGGGGAGGTCTTCGAGGGGCTCTGGAAAGACCGGGTCCAGGTGGCCATTAAGGTGATTTCTCGAGA
CTCTGATGAGAAAGTCCTGCCCGTTTCGGAGCTGCTGGACATCGCCTGGCAGGTGGCTGAGGGCATGTGTTACCTGGAGTCGCAGAATTACATCCACCGG
GACCTGGCCGCCAGGAACATCCTCGTCGGGGAAAACACCCTCTGCAAAGTTGGGGACTTCGGGTTAGCCAGGCTTATCAAGGAGGACGTCTACCTCTCCC
ATGACCACAATATCCCCTACAAGTGGACGGCCCCTGAAGCGCTCTCCCGAGGCCATTACTCCACCAAATCCGACGTCTGGTCCTTTGGGATTCTCCTGCA
TGAGATGTTCAGCAGGGGTCAGGTGCCCTACCCAGGCATGTCCAACCATGAGGCCTTCCTGAGGGTGGACGCCGGCTACCGCATGCCCTGCCCTCTGGAG
TGCCCGCCCAGCGTGCACAAGCTGATGCTGACATGCTGGTGCAGGGACCCCGAGCAGAGACCCTGCTTCAAGGCCCTGCGGGAGAGGCTCTCCAGCTTCA
CCAGCTACGAGAACCCGACCTGAGCTGCTGTGGAGCGGGCATGGCCGGGCCCTGCTGAGGAGGGGCCTGGGCAGAGGGCCTGGACCTGGGATCAAGGCCC
ACGCGCTTCCCTGGGGTTTACTGAGGTGATGGGTGCAGGAAAGGTTCACAAATGTGGAGTGTCTGCGTCCAATACACGCGTGTGCTCCTCTCCTTACTCC
ATCGTGTGTGCCTTGGGTCTCAGCTGCTGACACGCAGCCTGCTCTGGAGCCTGCAGATGAGATCCGGGAGACTGACACGAAGCCAGCAGAGGTCAGAGGG
GACTCTGACCACAGCCCGCTCTCTGGCTGTCTGTCTGCAGTGCCCGGCTGAGGGTGGGAGGCAAACACGCCTTGTTCCTGCTCTTCCCAGTTCAGCTTGG
TGGGAGAAAGTCATTCGCGTGGCTCGGGACGCTCATGTAAATTTGGTTTTGGTGCTCAAGGGTTCTTTCCTCCCAGGGGCAGGTGTTTCTTTCCTGTTTG
TCTTGTGTCTTGAGAGCTTGGCCTTATGACCAGTGAGAACTCTCTCCCTGGTCTCTGCCAGCCCAAGCATCACTGCCCGAGGCGCCAGCTCAGTTTCACC
GTCCACGTCCACAAGGGGCTTTTCCCACCTTCACCTTTGTCGCTGGGTCAGTGCTGGAAAGCGCCCCTCACTCCTGCGCTGACAAGGGCCCTTCTCTACT
GTCTGTGGGGTGGTTCCGGGCTGGGGGGGCTGCCTCCTTTGCACCTGATTTTGAAGGTGTCTCTTTCATCCATGGTTAAGTCATAAAAAGCTTATTGGTT
TTGGTTTTGACTCACCTGAAAGTTTTTTTGGTTTAAAAGAAGAATAGGCGGGGCACGGTGGCTCATGCCTGTAATCCCAGCACTTTGGGAGGCTGAGGCA
GGTGGATCACGAGGTCAGGAGATCGACACCATCCTGGCTAACACGGTGAAACCCCGTCTCTACTAAAAAATACAAAAAATTAGCTGGGTGTGGTGGTGGG
GGTGGGCGCCTGTAGTCCCAGCTACGTGGGAGGCTGAGGCAGCAGACTGGTGTGAACCCGGGAGGTGGAGCTTGCAGTGAGCCGAGATCGCGCCACTGCA
CTCCAGCCTGGGCGACAGAGCGAGACTCCATCTCAAAAAAAAAAAAAAAAAAAAGAAAGAAAGAAAAATAATTTTGGGAGTTTCTGGAAAGGTACTAGGA
TTTCTCAAAAGGATTTGTCTTCTGCCTTGTGAAAGACAGATGTCAGACTAATCAGGCTTATCCGATGTGCTACATGAGATGGAAAGCGTGTGAAATAGTA
AGTCACACTAAGTCTTCTGGAGGTTCTATTTACGGGTTTGGTTTTGATATGAATGTTGCAGAAACTCTATAAAATTCCTAGAAATCTGATACGTTATCCT
ATGATATATATCCTATCTCACATGCTATTCCTAGAAATCTGGTGCAATGTTATCAGTCATAATTTTGGTTATTACATTAAAATGCTGCATGCCACAGAAA
TAACCAAGTTTCCTTGCCAACCGTGTCACGAATATAATAAACTCTCATCAGATCCTGAGCCATGGCTATCTTAAGTCTTCGGTCATTCACAGTTATTATC
TTACTTTGATTTTTTCTGAAAGCTTTGCAATCAGCTAGTCCCAAATTGTTTATTCTTCAAAGAGATTCCTGGGAAGGATTCTGACAGGTGCTCTGGAGCA
CAGGTTTCTGGTTCAGGTCATAGCCTTGGA
  • PTK6.F9 (forward primer)
  • PTK6.R7 (reverse primer)
  • Alternative donor

Frame 0

CHAQLWVLQLGHSLVLPLRPPAMVSRDQAHLGP
KYVGLWDFKSRTDEELSFRAGDVFHVARKEEQWW
WATLLDEAGGAVAQGYVPHNYLAERETVESEPW
FFGCI
SRSEAVRRLQAEGNATGAFLIRCGTRRL
CGTTRSGGVPGAGCT*TRRCPSSACPSL*TTTGP
RACPTACGWPRPAGSTSLSPCPIGMTGRGRGRS
SRSAGSWGPATLGRSSRGSGKTGSRWPLR*FLE
TLMRKSCPFRSCWTSPGRWLRACVTWSRRITSTG
TWPPGTSSSGKTPSAKLGTSG*PGLSRRTSTSP
MTTISPTSGRPLKRSPEAITPPNPTSGPLGFSC
MRCSAGVRCPTQACPTMRPS*GWTPATACPALWS
ARPACTS*C*HAGAGTPSRDPASRPCGRGSPAS
PATRTRPELLWSGHGRALLRRGLGRGPGPGIKA
HALPWGLLR*WVQERFTNVECLRPIHACAPLLTP
SCVPWVSAADTQPALEPADEIRETDTKPAEVRG
DSDHSPLSGCLSAVPG*GWEANTPCSCSSQFSL
VGESHSRGSGRSCKFGFGAQGFFPPRGRCFFPVC
LVS*ELGLMTSENSLPGLCQPKHHCPRRQLSFT
VHVHKGLFPPSPLSLGQCWKAPLTPALTRALLY
CLWGGSGLGGLPPLHLILKVSLSSMVKS*KAYWF
WF*LT*KFFWFKRRIGGARWLMPVIPALWEAEA
GGSRGQEIDTILANTVKPRLY*KIQKISWVWWW
GWAPVVPATWEAEAADWCEPGRWSLQ*AEIAPLH
SSLGDRARLHLKKKKKKKKERKIILGVSGKVLG
FLKRICLLPCERQMSD*SGLSDVLHEMESV*NS
KSH*VFWRFYLRVWF*YECCRNSIKFLEI*YVIL
*YISYLTCYS*KSGAMLSVIILVITLKCCMPQK
*PSFLANRVTNIINSHQILSHGYLKSSVIHSYY
LTLIFSESFAIS*SQIVYSSKRFLGRILTGALEH
RFLVQVIAL

Frame 1

ATPSSGCSSWATAWSCRCARPPWCPGTRLTWAP
SMWASGTSSPGRTRS*ASARGTSSTWPGRRSSG
GGPRCWTRRVGPWPRAMCPTTTWPRGRRWSRNRG
SLAAS
PARKLCVGCRPRATPRAPS*SGAGHAGC
AALQDLAACRGPAAPERGGVLPQPARACELPQG
PEPVPRPAAGRALPEARA*APAPLG*LGEAEGGV
HALQEAGVRLLWGGLRGALERPGPGGH*GDFSR
L**ESPARFGAAGHRLAGG*GHVLPGVAELHPP
GPGRQEHPRRGKHPLQSWGLRVSQAYQGGRLPLP
*PQYPLQVDGP*SALPRPLLHQIRRLVLWDSPA
*DVQQGSGALPRHVQP*GLPEGGRRLPHALPSG
VPAQRAQADADMLVQGPRAETLLQGPAGEALQLH
QLREPDLSCCGAGMAGPC*GGAWAEGLDLGSRP
TRFPGVY*GDGCRKGSQMWSVCVQYTRVLLSLL
HRVCLGSQLLTRSLLWSLQMRSGRLTRSQQRSEG
TLTTARSLAVCLQCPAEGGRQTRLVPALPSSAW
WEKVIRVARDAHVNLVLVLKGSFLPGAGVSFLF
VLCLESLAL*PVRTLSLVSASPSITARGASSVSP
STSTRGFSHLHLCRWVSAGKRPSLLR*QGPFST
VCGVVPGWGGCLLCT*F*RCLFHPWLSHKKLIG
FGFDSPESFFGLKEE*AGHGGSCL*SQHFGRLRQ
VDHEVRRSTPSWLTR*NPVSTKKYKKLAGCGGG
GGRL*SQLRGRLRQQTGVNPGGGACSEPRSRHC
TPAWATERDSISKKKKKKRKKEK*FWEFLERY*D
FSKGFVFCLVKDRCQTNQAYPMCYMRWKACEIV
SHTKSSGGSIYGFGFDMNVAETL*NS*KSDTLS
YDIYPISHAIPRNLVQCYQS*FWLLH*NAACHRN
NQVSLPTVSRI**TLIRS*AMAILSLRSFTVII
LL*FFLKALQSASPKLFILQRDSWEGF*QVLWS
TGFWFRS*PW

Frame 2

PRPALGAPAGPQPGPAAAPARHGVPGPGSPGPQ
VCGPLGLQVPDGRGAELPRGGRLPRGQEGGAVV
VGHAAGRGGWGRGPGLCAPQLPGREGDGGVGTV
VLWLHL
PLGSCASAAGRGQRHGRLPDQVRDTQAV
RHYKIWRRAGGRLHLNEAVSFLSLPELVNYHRA
QSLSHGLRLAAPCRKHEPEPLPHWDDWERPREE
FTLCRKLGSGYFGEVFEGLWKDRVQVAIKVISRD
SDEKVLPVSELLDIAWQVAEGMCYLESQNYIHR
DLAARNILVGENTLCKVGDFGLARLIKEDVYLS
HDHNIPYKWTAPEALSRGHYSTKSDVWSFGILLH
EMFSRGQVPYPGMSNHEAFLRVDAGYRMPCPLE
CPPSVHKLMLTCWCRDPEQRPCFKALRERLSSF
TSYENPT*AAVERAWPGPAEEGPGQRAWTWDQGP
RASLGFTEVMGAGKVHKCGVSASNTRVCSSPYS
IVCALGLSC*HAACSGACR*DPGD*HEASRGQR
GL*PQPALWLSVCSARLRVGGKHALFLLFPVQLG
GRKSFAWLGTLM*IWFWCSRVLSSQGQVFLSCL
SCVLRAWPYDQ*ELSPWSLPAQASLPEAPAQFH
RPRPQGAFPTFTFVAGSVLESAPHSCADKGPSLL
SVGWFRAGGAASFAPDFEGVSFIHG*VIKSLLV
LVLTHLKVFLV*KKNRRGTVAHACNPSTLGG*G
RWITRSGDRHHPG*HGETPSLLKNTKN*LGVVVG
VGACSPSYVGG*GSRLV*TREVELAVSRDRATA
LQPGRQSETPSQKKKKKKERKKNNFGSFWKGTR
ISQKDLSSAL*KTDVRLIRLIRCAT*DGKRVK**
VTLSLLEVLFTGLVLI*MLQKLYKIPRNLIRYP
MIYILSHMLFLEIWCNVISHNFGYYIKMLHATE
ITKFPCQPCHEYNKLSSDPEPWLS*VFGHSQLLS
YFDFF*KLCNQLVPNCLFFKEIPGKDSDRCSGA
QVSGSGHSLG

Sequence PTK6.eAug05

GGCTGAGGAGGGTCACGCTCCTGAGATCCCGCGTTTCTCATCAGTGATTGGGCACCCACTGTGTGTGTGCGTGAGTCACAGGCTGTGTAGGGTTAGCTCC
CGGGGGACCCAAGCAACAAGCAGGGGAGGCTGTAAAGAGGCCGTCGGGCAGGGGCCCATGGTGGAGACCCCTCTATTACGTGCAGACCCACGACCTCGTT
AGCAGGGTCACGCGCAGACCCACGACCTCGTTAGTGAGCAGGGGTGCAGGGGTGGGGTCTGGCCGGAGGCTGGTCAGCTCCCAGGTGCAAAGACCCTGAG
TCCTGAGAACTCGCCCAGGTGGTTCTTTGGCTGCATCTCCCGCTCGGAAGCTGTGCGTCGGCTGCAGGCCGAGGGCAACGCCACGGGCGCCTTCCTGATC
AGGGTCAGCGAGAAGCCGAGTGCCGACTACGTCCTGTCGGTGCGGGACACGCAGGCTGTGCGGCACTACAAGATCTGGCGGCGTGCCGGGGGCCGGCTGC
ACCTGAACGAGGCGGTGTCCTTCCTCAGCCTGCCCGAGCTTGTGAACTACCACAGGGCCCAGAGCCTGTCCCACGGCCTGCGGCTGGCCGCGCCCTGCCG
GAAGCACGAGCCTGAGCCCCTGCCCCATTGGGATGACTGGGAGAGGCCGAGGGAGGAGTTCACGCTCTGCAGGAAGCTGGGGTCCGGCTACTTTGGGGAG
GTCTTCGAGGGGCTCTGGAAAGACCGGGTCCAGGTGGCCATTAAGGTGATTTCTCGAGACAACCTCCTGCACCAGCAGATGCTGCAGTCGGAGATCCAGG
CCATGAAGAAGCTGCGGCACAAACACATCCTGGCGCTGTACGCCGTGGTGTCCGTGGGGGACCCCGTGTACA
  • PTK6.F9 (forward primer)
  • PTK6.R7 (reverse primer)
  • Alternative donor

Frame 0

G*GGSRS*DPAFLISDWAPTVCVRESQAV*G*L
PGDPSNKQGRL*RGRRAGAHGGDPSITCRPTTSL
AGSRADPRPR**AGVQGWGLAGGWSAPRCKDPE
S*ELAQVVLWLHLPLGSCASAAGRGQRHGRLPD
QGQREAECRLRPVGAGHAGCAALQDLAACRGPAA
PERGGVLPQPARACELPQGPEPVPRPAAGRALP
EARA*APAPLG*LGEAEGGVHALQEAGVRLLWG
GLRGALERPGPGGH*GDFSRQPPAPADAAVGDPG
HEEAAAQTHPGAVRRGVRGGPRV

Frame 1

AEEGHAPEIPRFSSVIGHPLCVCVSHRLCRVSS
RGTQATSRGGCKEAVGQGPMVETPLLRADPRPR
*QGHAQTHDLVSEQGCRGGVWPEAGQLPGAKTLS
PENSPRWFFGCISRSEAVRRLQAEGNATGAFLI
RVSEKPSADYVLSVRDTQAVRHYKIWRRAGGRL
HLNEAVSFLSLPELVNYHRAQSLSHGLRLAAPCR
KHEPEPLPHWDDWERPREEFTLCRKLGSGYFGE
VFEGLWKDRVQVAIKVISRDNLLHQQMLQSEIQ
AMKKLRHKHILALYAVVSVGDPVY

Frame 2

LRRVTLLRSRVSHQ*LGTHCVCA*VTGCVGLAP
GGPKQQAGEAVKRPSGRGPWWRPLYYVQTHDLV
SRVTRRPTTSLVSRGAGVGSGRRLVSSQVQRP*
VLRTRPGGSLAASPARKLCVGCRPRATPRAPS*S
GSARSRVPTTSCRCGTRRLCGTTRSGGVPGAGC
T*TRRCPSSACPSL*TTTGPRACPTACGWPRPA
GSTSLSPCPIGMTGRGRGRSSRSAGSWGPATLGR
SSRGSGKTGSRWPLR*FLETTSCTSRCCSRRSR
P*RSCGTNTSWRCTPWCPWGTPCT

Sequence PTK6.cAug05

TGCCACGCCCAGCTCTGGGTGCTCCAGCTGGGCCACAGCCTGGTCCTGCCGCTGCGCCCGCCCGCCATGGTGTCCCGGGACCAGGCTCACCTGGGCCCCA
AGTATGTGGGCCTCTGGGACTTCAAGTCCCGGACGGACGAGGAGCTGAGCTTCCGCGCGGGGGACGTCTTCCACGTGGCCAGGAAGGAGGAGCAGTGGTG
GTGGGCCACGCTGCTGGACGAGGCGGGTGGGGCCGTGGCCCAGGGCTATGTGCCCCACAACTACCTGGCCGAGAGGGAGACGGTGGAGTCGGAACCGTGG
TTCTTTGGCTGCATCT
CCCGCTCGGAAGCTGTGCGTCGGCTGCAGGCCGAGGGCAACGCCACGGGCGCCTTCCTGATCAGGGTCAGCGAGAAGCCGAGTG
CCGACTACGTCCTGTCGG
TGCGGGACACGCAGGCTGTGCGGCACTACAAGATCTGGCGGCGTGCCGGGGGCCGGCTGCACCTGAACGAGGCGGTGTCCTT
CCTCAGCCTGCCCGAGCTTGTGAACTACCACAGGGCCCAGAGCCTGTCCCACGGCCTGCGGCTGGCCGCGCCCTGCCGGAAGCCAGCAGATGCTGCAGTC
GGAGATCCAGGCCATGAAGAAGCTGCGGCACAAACACATCCTGGCGCTGTACGCCGTGGTGTCCGTGGGGGACCCCGTGTACATCATCACGGAGCTCATG
GCCAAGGGCAGCCTGCTGGAGCTGCTCC
  • PTK6.F9 (forward primer)
  • PTK6.R7 (reverse primer)
  • Alternative donor

Frame 0

CHAQLWVLQLGHSLVLPLRPPAMVSRDQAHLGP
KYVGLWDFKSRTDEELSFRAGDVFHVARKEEQWW
WATLLDEAGGAVAQGYVPHNYLAERETVESEPW
FFGCI
SRSEAVRRLQAEGNATGAFLIRVSEKPS
ADYVLS
VRDTQAVRHYKIWRRAGGRLHLNEAVSF
LSLPELVNYHRAQSLSHGLRLAAPCRKPADAAV
GDPGHEEAAAQTHPGAVRRGVRGGPRVHHHGAH
GQGQPAGAA

Frame 1

ATPSSGCSSWATAWSCRCARPPWCPGTRLTWAP
SMWASGTSSPGRTRS*ASARGTSSTWPGRRSSG
GGPRCWTRRVGPWPRAMCPTTTWPRGRRWSRNRG
SLAAS
PARKLCVGCRPRATPRAPS*SGSARSRV
PTTSCR
CGTRRLCGTTRSGGVPGAGCT*TRRCP
SSACPSL*TTTGPRACPTACGWPRPAGSQQMLQS
EIQAMKKLRHKHILALYAVVSVGDPVYIITELM
AKGSLLELL

Frame 2

PRPALGAPAGPQPGPAAAPARHGVPGPGSPGPQ
VCGPLGLQVPDGRGAELPRGGRLPRGQEGGAVV
VGHAAGRGGWGRGPGLCAPQLPGREGDGGVGTV
VLWLHL
PLGSCASAAGRGQRHGRLPDQGQREAEC
RLRPVG
AGHAGCAALQDLAACRGPAAPERGGVL
PQPARACELPQGPEPVPRPAAGRALPEASRCCS
RRSRP*RSCGTNTSWRCTPWCPWGTPCTSSRSSW
PRAACWSCS

Sequence PTK6.aAug05

TGCCACGCCCAGCTCTGGGTGCTCCAGCTGGGCCACAGCCTGGTCCTGCCGCTGCGCCCGCCCGCCATGGTGTCCCGGGACCAGGCTCACCTGGGCCCCA
AGTATGTGGGCCTCTGGGACTTCAAGTCCCGGACGGACGAGGAGCTGAGCTTCCGCGCGGGGGACGTCTTCCACGTGGCCAGGAAGGAGGAGCAGTGGTG
GTGGGCCACGCTGCTGGACGAGGCGGGTGGGGCCGTGGCCCAGGGCTATGTGCCCCACAACTACCTGGCCGAGAGGGAGACGGTGGAGTCGGAACCGTGG
TTCTTTGGCTGCATCT
CCCGCTCGGAAGCTGTGCGTCGGCTGCAGGCCGAGGGCAACGCCACGGGCGCCTTCCTGATCAGGGTCAGCGAGAAGCCGAGTG
CCGACTACGTCCTGTCGG
TGCGGGACACGCAGGCTGTGCGGCACTACAAGATCTGGCGGCGTGCCGGGGGCCGGCTGCACCTGAACGAGGCGGTGTCCTT
CCTCAGCCTGCCCGAGCTTGTGAACTACCACAGGGCCCAGAGCCTGTCCCACGGCCTGCGGCTGGCCGCGCCCTGCCGGAAGCACGAGCCTGAGCCCCTG
CCCCATTGGGATGACTGGGAGAGGCCGAGGGAGGAGTTCACGCTCTGCAGGAAGCTGGGGTCCGGCTACTTTGGGGAGGTCTTCGAGGGGCTCTGGAAAG
ACCGGGTCCAGGTGGCCATTAAGGTGATTTCTCGAGACAACCTCCTGCACCAGCAGATGCTGCAGTCGGAGATCCAGGCCATGAAGAAGCTGCGGCACAA
ACACATCCTGGCGCTGTACGCCGTGGTGTCCGTGGGGGACCCCGTGTACATCATCACGGAGCTCATGGCCAAGGGCAGCCTGCTGGAGCTGCTCCGCGAC
TCTGATGAGAAAGTCCTGCCCGTTTCGGAGCTGCTGGACATCGCCTGGCAGGTGGCTGAGGGCATGTGTTACCTGGAGTCGCAGAATTACATCCACCGGG
ACCTGGCCGCCAGGAACATCCTCGTCGGGGAAAACACCCTCTGCAAAGTTGGGGACTTCGGGTTAGCCAGGCTTATCAAGGAGGACGTCTACCTCTCCCA
TGACCACAATATCCCCTACAAGTGGACGGCCCCTGAAGCGCTCTCCCGAGGCCATTACTCCACCAAATCCGACGTCTGGTCCTTTGGGATTCTCCTGCAT
GAGATGTTCAGCAGGGGTCAGGTGCCCTACCCAGGCATGTCCAACCATGAGGCCTTCCTGAGGGTGGACGCCGGCTACCGCATGCCCTGCCCTCTGGAGT
GCCCGCCCAGCGTGCACAAGCTGATGCTGACATGCTGGTGCAGGGACCCCGAGCAGAGACCCTGCTTCAAGGCCCTGCGGGAGAGGCTCTCCAGCTTCAC
CAGCTACGAGAACCCGACCTGAGCTGCTGTGGAGCGGGCATGGCCGGGCCCTGCTGAGGAGGGGCCTGGGCAGAGGGCCTGGACCTGGGATCAAGGCCCA
CGCGCTTCCCTGGGGTTTACTGAGGTGATGGGTGCAGGAAAGGTTCACAAATGTGGAGTGTCTGCGTCCAATACACGCGTGTGCTCCTCTCCTTACTCCA
TCGTGTGTGCCTTGGGTCTCAGCTGCTGACACGCAGCCTGCTCTGGAGCCTGCAGATGAGATCCGGGAGACTGACACGAAGCCAGCAGAGGTCAGAGGGG
ACTCTGACCACAGCCCGCTCTCTGGCTGTCTGTCTGCAGTGCCCGGCTGAGGGTGGGAGGCAAACACGCCTTGTTCCTGCTCTTCCCAGTTCAGCTTGGT
GGGAGAAAGTCATTCGCGTGGCTCGGGACGCTCATGTAAATTTGGTTTTGGTGCTCAAGGGTTCTTTCCTCCCAGGGGCAGGTGTTTCTTTCCTGTTTGT
CTTGTGTCTTGAGAGCTTGGCCTTATGACCAGTGAGAACTCTCTCCCTGGTCTCTGCCAGCCCAAGCATCACTGCCCGAGGCGCCAGCTCAGTTTCACCG
TCCACGTCCACAAGGGGCTTTTCCCACCTTCACCTTTGTCGCTGGGTCAGTGCTGGAAAGCGCCCCTCACTCCTGCGCTGACAAGGGCCCTTCTCTACTG
TCTGTGGGGTGGTTCCGGGCTGGGGGGGCTGCCTCCTTTGCACCTGATTTTGAAGGTGTCTCTTTCATCCATGGTTAAGTCATAAAAAGCTTATTGGTTT
TGGTTTTGACTCACCTGAAAGTTTTTTTGGTTTAAAAGAAGAATAGGCGGGGCACGGTGGCTCATGCCTGTAATCCCAGCACTTTGGGAGGCTGAGGCAG
GTGGATCACGAGGTCAGGAGATCGACACCATCCTGGCTAACACGGTGAAACCCCGTCTCTACTAAAAAATACAAAAAATTAGCTGGGTGTGGTGGTGGGG
GTGGGCGCCTGTAGTCCCAGCTACGTGGGAGGCTGAGGCAGCAGACTGGTGTGAACCCGGGAGGTGGAGCTTGCAGTGAGCCGAGATCGCGCCACTGCAC
TCCAGCCTGGGCGACAGAGCGAGACTCCATCTCAAAAAAAAAAAAAAAAAAAAGAAAGAAAGAAAAATAATTTTGGGAGTTTCTGGAAAGGTACTAGGAT
TTCTCAAAAGGATTTGTCTTCTGCCTTGTGAAAGACAGATGTCAGACTAATCAGGCTTATCCGATGTGCTACATGAGATGGAAAGCGTGTGAAATAGTAA
GTCACACTAAGTCTTCTGGAGGTTCTATTTACGGGTTTGGTTTTGATATGAATGTTGCAGAAACTCTATAAAATTCCTAGAAATCTGATACGTTATCCTA
TGATATATATCCTATCTCACATGCTATTCCTAGAAATCTGGTGCAATGTTATCAGTCATAATTTTGGTTATTACATTAAAATGCTGCATGCCACAGAAAT
AACCAAGTTTCCTTGCCAACCGTGTCACGAATATAATAAACTCTCATCAGATCCTGAGCCATGGCTATCTTAAGTCTTCGGTCATTCACAGTTATTATCT
TACTTTGATTTTTTCTGAAAGCTTTGCAATCAGCTAGTCCCAAATTGTTTATTCTTCAAAGAGATTCCTGGGAAGGATTCTGACAGGTGCTCTGGAGCAC
AGGTTTCTGGTTCAGGTCATAGCCTTGGA
  • PTK6.F9 (forward primer)
  • PTK6.R7 (reverse primer)
  • Alternative donor

Frame 0

CHAQLWVLQLGHSLVLPLRPPAMVSRDQAHLGP
KYVGLWDFKSRTDEELSFRAGDVFHVARKEEQWW
WATLLDEAGGAVAQGYVPHNYLAERETVESEPW
FFGCI
SRSEAVRRLQAEGNATGAFLIRVSEKPS
ADYVLS
VRDTQAVRHYKIWRRAGGRLHLNEAVSF
LSLPELVNYHRAQSLSHGLRLAAPCRKHEPEPL
PHWDDWERPREEFTLCRKLGSGYFGEVFEGLWK
DRVQVAIKVISRDNLLHQQMLQSEIQAMKKLRHK
HILALYAVVSVGDPVYIITELMAKGSLLELLRD
SDEKVLPVSELLDIAWQVAEGMCYLESQNYIHR
DLAARNILVGENTLCKVGDFGLARLIKEDVYLSH
DHNIPYKWTAPEALSRGHYSTKSDVWSFGILLH
EMFSRGQVPYPGMSNHEAFLRVDAGYRMPCPLE
CPPSVHKLMLTCWCRDPEQRPCFKALRERLSSFT
SYENPT*AAVERAWPGPAEEGPGQRAWTWDQGP
RASLGFTEVMGAGKVHKCGVSASNTRVCSSPYS
IVCALGLSC*HAACSGACR*DPGD*HEASRGQRG
L*PQPALWLSVCSARLRVGGKHALFLLFPVQLG
GRKSFAWLGTLM*IWFWCSRVLSSQGQVFLSCL
SCVLRAWPYDQ*ELSPWSLPAQASLPEAPAQFHR
PRPQGAFPTFTFVAGSVLESAPHSCADKGPSLL
SVGWFRAGGAASFAPDFEGVSFIHG*VIKSLLV
LVLTHLKVFLV*KKNRRGTVAHACNPSTLGG*GR
WITRSGDRHHPG*HGETPSLLKNTKN*LGVVVG
VGACSPSYVGG*GSRLV*TREVELAVSRDRATA
LQPGRQSETPSQKKKKKKERKKNNFGSFWKGTRI
SQKDLSSAL*KTDVRLIRLIRCAT*DGKRVK**
VTLSLLEVLFTGLVLI*MLQKLYKIPRNLIRYP
MIYILSHMLFLEIWCNVISHNFGYYIKMLHATEI
TKFPCQPCHEYNKLSSDPEPWLS*VFGHSQLLS
YFDFF*KLCNQLVPNCLFFKEIPGKDSDRCSGA
QVSGSGHSLG

Frame 1

ATPSSGCSSWATAWSCRCARPPWCPGTRLTWAP
SMWASGTSSPGRTRS*ASARGTSSTWPGRRSSG
GGPRCWTRRVGPWPRAMCPTTTWPRGRRWSRNRG
SLAAS
PARKLCVGCRPRATPRAPS*SGSARSRV
PTTSCR
CGTRRLCGTTRSGGVPGAGCT*TRRCP
SSACPSL*TTTGPRACPTACGWPRPAGSTSLSPC
PIGMTGRGRGRSSRSAGSWGPATLGRSSRGSGK
TGSRWPLR*FLETTSCTSRCCSRRSRP*RSCGT
NTSWRCTPWCPWGTPCTSSRSSWPRAACWSCSAT
LMRKSCPFRSCWTSPGRWLRACVTWSRRITSTG
TWPPGTSSSGKTPSAKLGTSG*PGLSRRTSTSP
MTTISPTSGRPLKRSPEAITPPNPTSGPLGFSCM
RCSAGVRCPTQACPTMRPS*GWTPATACPALWS
ARPACTS*C*HAGAGTPSRDPASRPCGRGSPAS
PATRTRPELLWSGHGRALLRRGLGRGPGPGIKAH
ALPWGLLR*WVQERFTNVECLRPIHACAPLLTP
SCVPWVSAADTQPALEPADEIRETDTKPAEVRG
DSDHSPLSGCLSAVPG*GWEANTPCSCSSQFSLV
GESHSRGSGRSCKFGFGAQGFFPPRGRCFFPVC
LVS*ELGLMTSENSLPGLCQPKHHCPRRQLSFT
VHVHKGLFPPSPLSLGQCWKAPLTPALTRALLYC
LWGGSGLGGLPPLHLILKVSLSSMVKS*KAYWF
WF*LT*KFFWFKRRIGGARWLMPVIPALWEAEA
GGSRGQEIDTILANTVKPRLY*KIQKISWVWWWG
WAPVVPATWEAEAADWCEPGRWSLQ*AEIAPLH
SSLGDRARLHLKKKKKKKKERKIILGVSGKVLG
FLKRICLLPCERQMSD*SGLSDVLHEMESV*NSK
SH*VFWRFYLRVWF*YECCRNSIKFLEI*YVIL
*YISYLTCYS*KSGAMLSVIILVITLKCCMPQK
*PSFLANRVTNIINSHQILSHGYLKSSVIHSYYL
TLIFSESFAIS*SQIVYSSKRFLGRILTGALEH
RFLVQVIAL

Frame 2

PRPALGAPAGPQPGPAAAPARHGVPGPGSPGPQ
VCGPLGLQVPDGRGAELPRGGRLPRGQEGGAVV
VGHAAGRGGWGRGPGLCAPQLPGREGDGGVGTV
VLWLHL
PLGSCASAAGRGQRHGRLPDQGQREAEC
RLRPVG
AGHAGCAALQDLAACRGPAAPERGGVL
PQPARACELPQGPEPVPRPAAGRALPEARA*AP
APLG*LGEAEGGVHALQEAGVRLLWGGLRGALER
PGPGGH*GDFSRQPPAPADAAVGDPGHEEAAAQ
THPGAVRRGVRGGPRVHHHGAHGQGQPAGAAPR
L**ESPARFGAAGHRLAGG*GHVLPGVAELHPPG
PGRQEHPRRGKHPLQSWGLRVSQAYQGGRLPLP
*PQYPLQVDGP*SALPRPLLHQIRRLVLWDSPA
*DVQQGSGALPRHVQP*GLPEGGRRLPHALPSGV
PAQRAQADADMLVQGPRAETLLQGPAGEALQLH
QLREPDLSCCGAGMAGPC*GGAWAEGLDLGSRP
TRFPGVY*GDGCRKGSQMWSVCVQYTRVLLSLLH
RVCLGSQLLTRSLLWSLQMRSGRLTRSQQRSEG
TLTTARSLAVCLQCPAEGGRQTRLVPALPSSAW
WEKVIRVARDAHVNLVLVLKGSFLPGAGVSFLFV
LCLESLAL*PVRTLSLVSASPSITARGASSVSP
STSTRGFSHLHLCRWVSAGKRPSLLR*QGPFST
VCGVVPGWGGCLLCT*F*RCLFHPWLSHKKLIGF
GFDSPESFFGLKEE*AGHGGSCL*SQHFGRLRQ
VDHEVRRSTPSWLTR*NPVSTKKYKKLAGCGGG
GGRL*SQLRGRLRQQTGVNPGGGACSEPRSRHCT
PAWATERDSISKKKKKKRKKEK*FWEFLERY*D
FSKGFVFCLVKDRCQTNQAYPMCYMRWKACEIV
SHTKSSGGSIYGFGFDMNVAETL*NS*KSDTLSY
DIYPISHAIPRNLVQCYQS*FWLLH*NAACHRN
NQVSLPTVSRI**TLIRS*AMAILSLRSFTVII
LL*FFLKALQSASPKLFILQRDSWEGF*QVLWST
GFWFRS*PW

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