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Sequence PTK6.dAug05
TGCCACGCCCAGCTCTGGGTGCTCCAGCTGGGCCACAGCCTGGTCCTGCCGCTGCGCCCGCCCGCCATGGTGTCCCGGGACCAGGCTCACCTGGGCCCCAAGTATGTGGGCCTCTGGGACTTCAAGTCCCGGACGGACGAGGAGCTGAGCTTCCGCGCGGGGGACGTCTTCCACGTGGCCAGGAAGGAGGAGCAGTGGTG
GTGGGCCACGCTGCTGGACGAGGCGGGTGGGGCCGTGGCCCAGGGCTATGTGCCCCACAACTACCTGGCCGAGAGGGAGACGGTGGAGTCGGAACCTGCG
GGACACGCAGGCTGTGCGGCACTACAAGATCTGGCGGCGTGCCGGGGGCCGGCTGCACCTGAACGAGGCGGTGTCCTTCCTCAGCCTGCCCGAGCTTGTG
AACTACCACAGGGCCCAGAGCCTGTCCCACGGCCTGCGGCTGGCCGCGCCCTGCCGGAAGCACGAGCCTGAGCCCCTGCCCCATTGGGATGACTGGGAGA
GGCCGAGGGAGGAGTTCACGCTCTGCAGGAAGCTGGGGTCCGGCTACTTTGGGGAGGTCTTCGAGGGGCTCTGGAAAGACCGGGTCCAGGTGGCCATTAA
GGTGATTTCTCGAGACAACCTCCTGCACCAGCAGATGCTGCAGTCGGAGATCCAGGCCATGAAGAAGCTGCGGCACAAACACATCCTGGCGCTGTACGCC
GTGGTGTCCGTGGGGGACCCCGTGTACATCATCACGGAGCTCATGGCCAAGGGCAGCCTGCTGGAGCTGCTCCGCGACTCTGATGAGAAAGTCCTGCCCG
TTTCGGAGCTGCTGGACATCGCCTGGCAGGTGGCTGAGGGCATGTGTTACCTGGAGTCGCAGAATTACATCCACCGGGACCTGGCCGCCAGGAACATCCT
CGTCGGGGAA
- PTK6.F8 (forward primer)
- PTK6.R8 (reverse primer)
- Single exon skipping
Frame 0
CHAQLWVLQLGHSLVLPLRPPAMVSRDQAHLGPKYVGLWDFKSRTDEELSFRAGDVFHVARKEEQWW
WATLLDEAGGAVAQGYVPHNYLAERETVESEPA
GHAGCAALQDLAACRGPAAPERGGVLPQPARAC
ELPQGPEPVPRPAAGRALPEARA*APAPLG*LGE
AEGGVHALQEAGVRLLWGGLRGALERPGPGGH*
GDFSRQPPAPADAAVGDPGHEEAAAQTHPGAVR
RGVRGGPRVHHHGAHGQGQPAGAAPRL**ESPAR
FGAAGHRLAGG*GHVLPGVAELHPPGPGRQEHP
RRG
Frame 1
ATPSSGCSSWATAWSCRCARPPWCPGTRLTWAPSMWASGTSSPGRTRS*ASARGTSSTWPGRRSSG
GGPRCWTRRVGPWPRAMCPTTTWPRGRRWSRNLR
DTQAVRHYKIWRRAGGRLHLNEAVSFLSLPELV
NYHRAQSLSHGLRLAAPCRKHEPEPLPHWDDWE
RPREEFTLCRKLGSGYFGEVFEGLWKDRVQVAIK
VISRDNLLHQQMLQSEIQAMKKLRHKHILALYA
VVSVGDPVYIITELMAKGSLLELLRDSDEKVLP
VSELLDIAWQVAEGMCYLESQNYIHRDLAARNIL
VGE
Frame 2
PRPALGAPAGPQPGPAAAPARHGVPGPGSPGPQVCGPLGLQVPDGRGAELPRGGRLPRGQEGGAVV
VGHAAGRGGWGRGPGLCAPQLPGREGDGGVGTC
GTRRLCGTTRSGGVPGAGCT*TRRCPSSACPSL*
TTTGPRACPTACGWPRPAGSTSLSPCPIGMTGR
GRGRSSRSAGSWGPATLGRSSRGSGKTGSRWPL
R*FLETTSCTSRCCSRRSRP*RSCGTNTSWRCTP
WCPWGTPCTSSRSSWPRAACWSCSATLMRKSCP
FRSCWTSPGRWLRACVTWSRRITSTGTWPPGTS
SSG
Sequence PTK6.cAug05
TGCCACGCCCAGCTCTGGGTGCTCCAGCTGGGCCACAGCCTGGTCCTGCCGCTGCGCCCGCCCGCCATGGTGTCCCGGGACCAGGCTCACCTGGGCCCCAAGTATGTGGGCCTCTGGGACTTCAAGTCCCGGACGGACGAGGAGCTGAGCTTCCGCGCGGGGGACGTCTTCCACGTGGCCAGGAAGGAGGAGCAGTGGTG
GTGGGCCACGCTGCTGGACGAGGCGGGTGGGGCCGTGGCCCAGGGCTATGTGCCCCACAACTACCTGGCCGAGAGGGAGACGGTGGAGTCGGAACCGTGG
TTCTTTGGCTGCATCTCCCGCTCGGAAGCTGTGCGTCGGCTGCAGGCCGAGGGCAACGCCACGGGCGCCTTCCTGATCAGGGTCAGCGAGAAGCCGAGTG
CCGACTACGTCCTGTCGGTGCGGGACACGCAGGCTGTGCGGCACTACAAGATCTGGCGGCGTGCCGGGGGCCGGCTGCACCTGAACGAGGCGGTGTCCTT
CCTCAGCCTGCCCGAGCTTGTGAACTACCACAGGGCCCAGAGCCTGTCCCACGGCCTGCGGCTGGCCGCGCCCTGCCGGAAGCCAGCAGATGCTGCAGTC
GGAGATCCAGGCCATGAAGAAGCTGCGGCACAAACACATCCTGGCGCTGTACGCCGTGGTGTCCGTGGGGGACCCCGTGTACATCATCACGGAGCTCATG
GCCAAGGGCAGCCTGCTGGAGCTGCTCC
- PTK6.F8 (forward primer)
- PTK6.R8 (reverse primer)
- Single exon skipping
Frame 0
CHAQLWVLQLGHSLVLPLRPPAMVSRDQAHLGPKYVGLWDFKSRTDEELSFRAGDVFHVARKEEQWW
WATLLDEAGGAVAQGYVPHNYLAERETVESEPW
FFGCISRSEAVRRLQAEGNATGAFLIRVSEKPS
ADYVLSVRDTQAVRHYKIWRRAGGRLHLNEAVSF
LSLPELVNYHRAQSLSHGLRLAAPCRKPADAAV
GDPGHEEAAAQTHPGAVRRGVRGGPRVHHHGAH
GQGQPAGAA
Frame 1
ATPSSGCSSWATAWSCRCARPPWCPGTRLTWAPSMWASGTSSPGRTRS*ASARGTSSTWPGRRSSG
GGPRCWTRRVGPWPRAMCPTTTWPRGRRWSRNRG
SLAASPARKLCVGCRPRATPRAPS*SGSARSRV
PTTSCRCGTRRLCGTTRSGGVPGAGCT*TRRCP
SSACPSL*TTTGPRACPTACGWPRPAGSQQMLQS
EIQAMKKLRHKHILALYAVVSVGDPVYIITELM
AKGSLLELL
Frame 2
PRPALGAPAGPQPGPAAAPARHGVPGPGSPGPQVCGPLGLQVPDGRGAELPRGGRLPRGQEGGAVV
VGHAAGRGGWGRGPGLCAPQLPGREGDGGVGTV
VLWLHLPLGSCASAAGRGQRHGRLPDQGQREAEC
RLRPVGAGHAGCAALQDLAACRGPAAPERGGVL
PQPARACELPQGPEPVPRPAAGRALPEASRCCS
RRSRP*RSCGTNTSWRCTPWCPWGTPCTSSRSSW
PRAACWSCS
Sequence PTK6.aAug05
TGCCACGCCCAGCTCTGGGTGCTCCAGCTGGGCCACAGCCTGGTCCTGCCGCTGCGCCCGCCCGCCATGGTGTCCCGGGACCAGGCTCACCTGGGCCCCAAGTATGTGGGCCTCTGGGACTTCAAGTCCCGGACGGACGAGGAGCTGAGCTTCCGCGCGGGGGACGTCTTCCACGTGGCCAGGAAGGAGGAGCAGTGGTG
GTGGGCCACGCTGCTGGACGAGGCGGGTGGGGCCGTGGCCCAGGGCTATGTGCCCCACAACTACCTGGCCGAGAGGGAGACGGTGGAGTCGGAACCGTGG
TTCTTTGGCTGCATCTCCCGCTCGGAAGCTGTGCGTCGGCTGCAGGCCGAGGGCAACGCCACGGGCGCCTTCCTGATCAGGGTCAGCGAGAAGCCGAGTG
CCGACTACGTCCTGTCGGTGCGGGACACGCAGGCTGTGCGGCACTACAAGATCTGGCGGCGTGCCGGGGGCCGGCTGCACCTGAACGAGGCGGTGTCCTT
CCTCAGCCTGCCCGAGCTTGTGAACTACCACAGGGCCCAGAGCCTGTCCCACGGCCTGCGGCTGGCCGCGCCCTGCCGGAAGCACGAGCCTGAGCCCCTG
CCCCATTGGGATGACTGGGAGAGGCCGAGGGAGGAGTTCACGCTCTGCAGGAAGCTGGGGTCCGGCTACTTTGGGGAGGTCTTCGAGGGGCTCTGGAAAG
ACCGGGTCCAGGTGGCCATTAAGGTGATTTCTCGAGACAACCTCCTGCACCAGCAGATGCTGCAGTCGGAGATCCAGGCCATGAAGAAGCTGCGGCACAA
ACACATCCTGGCGCTGTACGCCGTGGTGTCCGTGGGGGACCCCGTGTACATCATCACGGAGCTCATGGCCAAGGGCAGCCTGCTGGAGCTGCTCCGCGAC
TCTGATGAGAAAGTCCTGCCCGTTTCGGAGCTGCTGGACATCGCCTGGCAGGTGGCTGAGGGCATGTGTTACCTGGAGTCGCAGAATTACATCCACCGGG
ACCTGGCCGCCAGGAACATCCTCGTCGGGGAAAACACCCTCTGCAAAGTTGGGGACTTCGGGTTAGCCAGGCTTATCAAGGAGGACGTCTACCTCTCCCA
TGACCACAATATCCCCTACAAGTGGACGGCCCCTGAAGCGCTCTCCCGAGGCCATTACTCCACCAAATCCGACGTCTGGTCCTTTGGGATTCTCCTGCAT
GAGATGTTCAGCAGGGGTCAGGTGCCCTACCCAGGCATGTCCAACCATGAGGCCTTCCTGAGGGTGGACGCCGGCTACCGCATGCCCTGCCCTCTGGAGT
GCCCGCCCAGCGTGCACAAGCTGATGCTGACATGCTGGTGCAGGGACCCCGAGCAGAGACCCTGCTTCAAGGCCCTGCGGGAGAGGCTCTCCAGCTTCAC
CAGCTACGAGAACCCGACCTGAGCTGCTGTGGAGCGGGCATGGCCGGGCCCTGCTGAGGAGGGGCCTGGGCAGAGGGCCTGGACCTGGGATCAAGGCCCA
CGCGCTTCCCTGGGGTTTACTGAGGTGATGGGTGCAGGAAAGGTTCACAAATGTGGAGTGTCTGCGTCCAATACACGCGTGTGCTCCTCTCCTTACTCCA
TCGTGTGTGCCTTGGGTCTCAGCTGCTGACACGCAGCCTGCTCTGGAGCCTGCAGATGAGATCCGGGAGACTGACACGAAGCCAGCAGAGGTCAGAGGGG
ACTCTGACCACAGCCCGCTCTCTGGCTGTCTGTCTGCAGTGCCCGGCTGAGGGTGGGAGGCAAACACGCCTTGTTCCTGCTCTTCCCAGTTCAGCTTGGT
GGGAGAAAGTCATTCGCGTGGCTCGGGACGCTCATGTAAATTTGGTTTTGGTGCTCAAGGGTTCTTTCCTCCCAGGGGCAGGTGTTTCTTTCCTGTTTGT
CTTGTGTCTTGAGAGCTTGGCCTTATGACCAGTGAGAACTCTCTCCCTGGTCTCTGCCAGCCCAAGCATCACTGCCCGAGGCGCCAGCTCAGTTTCACCG
TCCACGTCCACAAGGGGCTTTTCCCACCTTCACCTTTGTCGCTGGGTCAGTGCTGGAAAGCGCCCCTCACTCCTGCGCTGACAAGGGCCCTTCTCTACTG
TCTGTGGGGTGGTTCCGGGCTGGGGGGGCTGCCTCCTTTGCACCTGATTTTGAAGGTGTCTCTTTCATCCATGGTTAAGTCATAAAAAGCTTATTGGTTT
TGGTTTTGACTCACCTGAAAGTTTTTTTGGTTTAAAAGAAGAATAGGCGGGGCACGGTGGCTCATGCCTGTAATCCCAGCACTTTGGGAGGCTGAGGCAG
GTGGATCACGAGGTCAGGAGATCGACACCATCCTGGCTAACACGGTGAAACCCCGTCTCTACTAAAAAATACAAAAAATTAGCTGGGTGTGGTGGTGGGG
GTGGGCGCCTGTAGTCCCAGCTACGTGGGAGGCTGAGGCAGCAGACTGGTGTGAACCCGGGAGGTGGAGCTTGCAGTGAGCCGAGATCGCGCCACTGCAC
TCCAGCCTGGGCGACAGAGCGAGACTCCATCTCAAAAAAAAAAAAAAAAAAAAGAAAGAAAGAAAAATAATTTTGGGAGTTTCTGGAAAGGTACTAGGAT
TTCTCAAAAGGATTTGTCTTCTGCCTTGTGAAAGACAGATGTCAGACTAATCAGGCTTATCCGATGTGCTACATGAGATGGAAAGCGTGTGAAATAGTAA
GTCACACTAAGTCTTCTGGAGGTTCTATTTACGGGTTTGGTTTTGATATGAATGTTGCAGAAACTCTATAAAATTCCTAGAAATCTGATACGTTATCCTA
TGATATATATCCTATCTCACATGCTATTCCTAGAAATCTGGTGCAATGTTATCAGTCATAATTTTGGTTATTACATTAAAATGCTGCATGCCACAGAAAT
AACCAAGTTTCCTTGCCAACCGTGTCACGAATATAATAAACTCTCATCAGATCCTGAGCCATGGCTATCTTAAGTCTTCGGTCATTCACAGTTATTATCT
TACTTTGATTTTTTCTGAAAGCTTTGCAATCAGCTAGTCCCAAATTGTTTATTCTTCAAAGAGATTCCTGGGAAGGATTCTGACAGGTGCTCTGGAGCAC
AGGTTTCTGGTTCAGGTCATAGCCTTGGA
- PTK6.F8 (forward primer)
- PTK6.R8 (reverse primer)
- Single exon skipping
Frame 0
CHAQLWVLQLGHSLVLPLRPPAMVSRDQAHLGPKYVGLWDFKSRTDEELSFRAGDVFHVARKEEQWW
WATLLDEAGGAVAQGYVPHNYLAERETVESEPW
FFGCISRSEAVRRLQAEGNATGAFLIRVSEKPS
ADYVLSVRDTQAVRHYKIWRRAGGRLHLNEAVSF
LSLPELVNYHRAQSLSHGLRLAAPCRKHEPEPL
PHWDDWERPREEFTLCRKLGSGYFGEVFEGLWK
DRVQVAIKVISRDNLLHQQMLQSEIQAMKKLRHK
HILALYAVVSVGDPVYIITELMAKGSLLELLRD
SDEKVLPVSELLDIAWQVAEGMCYLESQNYIHR
DLAARNILVGENTLCKVGDFGLARLIKEDVYLSH
DHNIPYKWTAPEALSRGHYSTKSDVWSFGILLH
EMFSRGQVPYPGMSNHEAFLRVDAGYRMPCPLE
CPPSVHKLMLTCWCRDPEQRPCFKALRERLSSFT
SYENPT*AAVERAWPGPAEEGPGQRAWTWDQGP
RASLGFTEVMGAGKVHKCGVSASNTRVCSSPYS
IVCALGLSC*HAACSGACR*DPGD*HEASRGQRG
L*PQPALWLSVCSARLRVGGKHALFLLFPVQLG
GRKSFAWLGTLM*IWFWCSRVLSSQGQVFLSCL
SCVLRAWPYDQ*ELSPWSLPAQASLPEAPAQFHR
PRPQGAFPTFTFVAGSVLESAPHSCADKGPSLL
SVGWFRAGGAASFAPDFEGVSFIHG*VIKSLLV
LVLTHLKVFLV*KKNRRGTVAHACNPSTLGG*GR
WITRSGDRHHPG*HGETPSLLKNTKN*LGVVVG
VGACSPSYVGG*GSRLV*TREVELAVSRDRATA
LQPGRQSETPSQKKKKKKERKKNNFGSFWKGTRI
SQKDLSSAL*KTDVRLIRLIRCAT*DGKRVK**
VTLSLLEVLFTGLVLI*MLQKLYKIPRNLIRYP
MIYILSHMLFLEIWCNVISHNFGYYIKMLHATEI
TKFPCQPCHEYNKLSSDPEPWLS*VFGHSQLLS
YFDFF*KLCNQLVPNCLFFKEIPGKDSDRCSGA
QVSGSGHSLG
Frame 1
ATPSSGCSSWATAWSCRCARPPWCPGTRLTWAPSMWASGTSSPGRTRS*ASARGTSSTWPGRRSSG
GGPRCWTRRVGPWPRAMCPTTTWPRGRRWSRNRG
SLAASPARKLCVGCRPRATPRAPS*SGSARSRV
PTTSCRCGTRRLCGTTRSGGVPGAGCT*TRRCP
SSACPSL*TTTGPRACPTACGWPRPAGSTSLSPC
PIGMTGRGRGRSSRSAGSWGPATLGRSSRGSGK
TGSRWPLR*FLETTSCTSRCCSRRSRP*RSCGT
NTSWRCTPWCPWGTPCTSSRSSWPRAACWSCSAT
LMRKSCPFRSCWTSPGRWLRACVTWSRRITSTG
TWPPGTSSSGKTPSAKLGTSG*PGLSRRTSTSP
MTTISPTSGRPLKRSPEAITPPNPTSGPLGFSCM
RCSAGVRCPTQACPTMRPS*GWTPATACPALWS
ARPACTS*C*HAGAGTPSRDPASRPCGRGSPAS
PATRTRPELLWSGHGRALLRRGLGRGPGPGIKAH
ALPWGLLR*WVQERFTNVECLRPIHACAPLLTP
SCVPWVSAADTQPALEPADEIRETDTKPAEVRG
DSDHSPLSGCLSAVPG*GWEANTPCSCSSQFSLV
GESHSRGSGRSCKFGFGAQGFFPPRGRCFFPVC
LVS*ELGLMTSENSLPGLCQPKHHCPRRQLSFT
VHVHKGLFPPSPLSLGQCWKAPLTPALTRALLYC
LWGGSGLGGLPPLHLILKVSLSSMVKS*KAYWF
WF*LT*KFFWFKRRIGGARWLMPVIPALWEAEA
GGSRGQEIDTILANTVKPRLY*KIQKISWVWWWG
WAPVVPATWEAEAADWCEPGRWSLQ*AEIAPLH
SSLGDRARLHLKKKKKKKKERKIILGVSGKVLG
FLKRICLLPCERQMSD*SGLSDVLHEMESV*NSK
SH*VFWRFYLRVWF*YECCRNSIKFLEI*YVIL
*YISYLTCYS*KSGAMLSVIILVITLKCCMPQK
*PSFLANRVTNIINSHQILSHGYLKSSVIHSYYL
TLIFSESFAIS*SQIVYSSKRFLGRILTGALEH
RFLVQVIAL
Frame 2
PRPALGAPAGPQPGPAAAPARHGVPGPGSPGPQVCGPLGLQVPDGRGAELPRGGRLPRGQEGGAVV
VGHAAGRGGWGRGPGLCAPQLPGREGDGGVGTV
VLWLHLPLGSCASAAGRGQRHGRLPDQGQREAEC
RLRPVGAGHAGCAALQDLAACRGPAAPERGGVL
PQPARACELPQGPEPVPRPAAGRALPEARA*AP
APLG*LGEAEGGVHALQEAGVRLLWGGLRGALER
PGPGGH*GDFSRQPPAPADAAVGDPGHEEAAAQ
THPGAVRRGVRGGPRVHHHGAHGQGQPAGAAPR
L**ESPARFGAAGHRLAGG*GHVLPGVAELHPPG
PGRQEHPRRGKHPLQSWGLRVSQAYQGGRLPLP
*PQYPLQVDGP*SALPRPLLHQIRRLVLWDSPA
*DVQQGSGALPRHVQP*GLPEGGRRLPHALPSGV
PAQRAQADADMLVQGPRAETLLQGPAGEALQLH
QLREPDLSCCGAGMAGPC*GGAWAEGLDLGSRP
TRFPGVY*GDGCRKGSQMWSVCVQYTRVLLSLLH
RVCLGSQLLTRSLLWSLQMRSGRLTRSQQRSEG
TLTTARSLAVCLQCPAEGGRQTRLVPALPSSAW
WEKVIRVARDAHVNLVLVLKGSFLPGAGVSFLFV
LCLESLAL*PVRTLSLVSASPSITARGASSVSP
STSTRGFSHLHLCRWVSAGKRPSLLR*QGPFST
VCGVVPGWGGCLLCT*F*RCLFHPWLSHKKLIGF
GFDSPESFFGLKEE*AGHGGSCL*SQHFGRLRQ
VDHEVRRSTPSWLTR*NPVSTKKYKKLAGCGGG
GGRL*SQLRGRLRQQTGVNPGGGACSEPRSRHCT
PAWATERDSISKKKKKKRKKEK*FWEFLERY*D
FSKGFVFCLVKDRCQTNQAYPMCYMRWKACEIV
SHTKSSGGSIYGFGFDMNVAETL*NS*KSDTLSY
DIYPISHAIPRNLVQCYQS*FWLLH*NAACHRN
NQVSLPTVSRI**TLIRS*AMAILSLRSFTVII
LL*FFLKALQSASPKLFILQRDSWEGF*QVLWST
GFWFRS*PW
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