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Sequence SHBG.eAug05
CCGCGGTTCAAAGGCTCCCCCGCAGTGCTTTTTAAATTGACATATGCAGTGATAACCTGCTTTAGCCTCAGGCTCACTCACCCGCCCAGACCCTGGAGTGCCCACGACCCTCCGGCTGTCCACCTCAGCAATGGCCCAGGACAAGAGCCTATCGCTGTCATGACCTTTGACCTCACCAAGATCACAAAAACCTCCTCCTC
CTTTGAGGTTCGAACCTGGGACCCAGAGGGAGTGATTTTTTATGGGGATACCAACCCTAAGGATGACTGGTTTATGCTGGGACTTCGAGACGGCAGGCCT
GAGATCCAACTGCACAATCACTGGGCCCAGCTTACGGTGGGTGCTGGACCACGGCTGGATGATGGGAGATGGCACCAGGTGGAAGTCAAGATGGAGGGGG
ACTCTGTGCTGCTGGAGGTGGATGGGGAGGAGGTGCTGCGCCTGAGACAGGTCTCTGGGCCCCTGACCAGCAAACGCCATCCCATCATGAGGATTGCGCT
TGGGGGGCTGCTCTTCCCCGCTTCCAACCTTCGGTTGCCGCTGGTTCCTGCCCTGGATGGCTGCCTGCGCCGGGATTCCTGGCTGGACAAACAGGCCGAG
ATCTCAGCATCTGCCCCCACTAGCCTCAGAAGCTGTGATGTAGAATCAAATCCCGGGATATTTCTCCCTCCAGGGACTCAGGCAGAATTCAATCTCCGAG
ACATTCCCCAGCCTCATGCAGAGCCCTGGGCCTTCTCTTTGGACCTGGGACTCAAGCAGGCAGCAGGCTCAGGCCACCTCCTTGCTCTTGGGACACCAGA
GAACCCATCTTGGCTCAGTCTCCACCTCCAAGATCAAGAGAAGACTCTTCCACCTCTTTTTGCCTGAATGGCCTTTGGGCACAAGGTCAGAGGCTGGATG
TGGACCAGGCCCTGAACAGAAGCCATGAGATCTGGACTCACAGCTGCCCCCAGAGCCCAGGCAATGGCACTGACGCTTCCCATTAAAGCTCCACCTAAGA
ACCCCCTTTGAAAGTT
- SHBG.F2 (forward primer)
- SHBG.R2 (reverse primer)
- Single exon skipping
Frame 0
PRFKGSPAVLFKLTYAVITCFSLRLTHPPRPWSAHDPPAVHLSNGPGQEPIAVMTFDLTKITKTSSS
FEVRTWDPEGVIFYGDTNPKDDWFMLGLRDGRP
EIQLHNHWAQLTVGAGPRLDDGRWHQVEVKMEG
DSVLLEVDGEEVLRLRQVSGPLTSKRHPIMRIAL
GGLLFPASNLRLPLVPALDGCLRRDSWLDKQAE
ISASAPTSLRSCDVESNPGIFLPPGTQAEFNLR
DIPQPHAEPWAFSLDLGLKQAAGSGHLLALGTPE
NPSWLSLHLQDQEKTLPPLFA*MAFGHKVRGWM
WTRP*TEAMRSGLTAAPRAQAMALTLPIKAPPK
NPL*K
Frame 1
RGSKAPPQCFLN*HMQ**PALASGSLTRPDPGVPTTLRLSTSAMAQDKSLSLS*PLTSPRSQKPPP
PLRFEPGTQRE*FFMGIPTLRMTGLCWDFETAGL
RSNCTITGPSLRWVLDHGWMMGDGTRWKSRWRG
TLCCWRWMGRRCCA*DRSLGP*PANAIPS*GLR
LGGCSSPLPTFGCRWFLPWMAACAGIPGWTNRPR
SQHLPPLASEAVM*NQIPGYFSLQGLRQNSISE
TFPSLMQSPGPSLWTWDSSRQQAQATSLLLGHQ
RTHLGSVSTSKIKRRLFHLFLPEWPLGTRSEAGC
GPGPEQKP*DLDSQLPPEPRQWH*RFPLKLHLR
TPFES
Frame 2
AVQRLPRSAF*IDICSDNLL*PQAHSPAQTLECPRPSGCPPQQWPRTRAYRCHDL*PHQDHKNLLL
L*GSNLGPRGSDFLWGYQP*G*LVYAGTSRRQA
*DPTAQSLGPAYGGCWTTAG*WEMAPGGSQDGGG
LCAAGGGWGGGAAPETGLWAPDQQTPSHHEDCA
WGAALPRFQPSVAAGSCPGWLPAPGFLAGQTGR
DLSICPH*PQKL*CRIKSRDISPSRDSGRIQSPR
HSPASCRALGLLFGPGTQAGSRLRPPPCSWDTR
EPILAQSPPPRSREDSSTSFCLNGLWAQGQRLD
VDQALNRSHEIWTHSCPQSPGNGTDASH*SST*E
PPLKV
Sequence SHBG.dAug05
TGAGCCGCCGAGTGGACAGTGGCTGATTATGGAGAGCAGAGGCCCACTGGCTACCTCGCGCCTGCTGCTGTTGCTGCTGTTGCTACTACTGCGTCACACCCGCCAGGGATGGGCCCTGAGACCTGTTCTCCCCACCCAGAGTGCCCACGACCCTCCGGCTGTCCACCTCAGCAATGGCCCAGGACAAGAGCCTATCGCTG
TCATGACCTTTGACCTCACCAAGATCACAAAAACCTCCTCCTCCTTTGAGGTTCGAACCTGGGACCCAGAGGGAGTGATTTTTTATGGGGATACCAACCC
TAAGGATGACTGGTTTATGCTGGGACTTCGAGACGGCAGGCCTGAGATCCAACTGCACAATCACTGGGCCCAGCTTACGGTGGGTGCTGGACCACGGCTG
GATGATGGGAGATGGCACCAGGTGGAAGTCAAGATGGAGGGGGACTCTGTGCTGCTGGAGGTGGATGGGGAGGAGGTGCTGCGCCTGAGACAGGTCTCTG
GGCCCCTGACCAGCAAACGCCATCCCATCATGAGGATTGCGCTTGGGGGGCTGCTCTTCCCCGCTTCCAACCTTCGGTTGCCGCTGGTTCCTGCCCTGGA
TGGCTGCCTGCGCCGGGATTCCTGGCTGGACAAACAGGCCGAGATCTCAGCATCTGCCCCCACTAGCCTCAGAAGCTGTGATGTAGAATCAAATCCCGGG
ATATTTCTCCCTCCAGGGACTCAGGCAGAATTCAATCTCCGAGACATTCCCCAGCCTCATGCAGAGCCCTGGGCCTTCTCTTTGGACCTGGGACTCAAGC
AGGCAGCAGGCTCAGGCCACCTCCTTGCTCTTGGGACACCAGAGAACCCATCTTGGCTCAGTCTCCACCTCCAAGATCAAGAGAAGACTCTTCCACCTCT
TTTTGCCTGAATGGCCTTTGGGCACAAGGTCAGAGGCTGGATGTGGACCAGGCCCTGAACAGAAGCCATGAGATCTGGACTCACAGCTGCCCCCAGAGCC
CAGGCAATGGCACTGACGCTTCCCATTAAAGCTCCACCTAAGAACCCCCTTTGAAAGTT
- SHBG.F2 (forward primer)
- SHBG.R2 (reverse primer)
- Single exon skipping
Frame 0
*AAEWTVADYGEQRPTGYLAPAAVAAVATTASHPPGMGPETCSPHPECPRPSGCPPQQWPRTRAYRC
HDL*PHQDHKNLLLL*GSNLGPRGSDFLWGYQP
*G*LVYAGTSRRQA*DPTAQSLGPAYGGCWTTA
G*WEMAPGGSQDGGGLCAAGGGWGGGAAPETGLW
APDQQTPSHHEDCAWGAALPRFQPSVAAGSCPG
WLPAPGFLAGQTGRDLSICPH*PQKL*CRIKSR
DISPSRDSGRIQSPRHSPASCRALGLLFGPGTQA
GSRLRPPPCSWDTREPILAQSPPPRSREDSSTS
FCLNGLWAQGQRLDVDQALNRSHEIWTHSCPQS
PGNGTDASH*SST*EPPLKV
Frame 1
EPPSGQWLIMESRGPLATSRLLLLLLLLLLRHTRQGWALRPVLPTQSAHDPPAVHLSNGPGQEPIA
VMTFDLTKITKTSSSFEVRTWDPEGVIFYGDTNP
KDDWFMLGLRDGRPEIQLHNHWAQLTVGAGPRL
DDGRWHQVEVKMEGDSVLLEVDGEEVLRLRQVS
GPLTSKRHPIMRIALGGLLFPASNLRLPLVPALD
GCLRRDSWLDKQAEISASAPTSLRSCDVESNPG
IFLPPGTQAEFNLRDIPQPHAEPWAFSLDLGLK
QAAGSGHLLALGTPENPSWLSLHLQDQEKTLPPL
FA*MAFGHKVRGWMWTRP*TEAMRSGLTAAPRA
QAMALTLPIKAPPKNPL*K
Frame 2
SRRVDSG*LWRAEAHWLPRACCCCCCCYYCVTPARDGP*DLFSPPRVPTTLRLSTSAMAQDKSLSL
S*PLTSPRSQKPPPPLRFEPGTQRE*FFMGIPT
LRMTGLCWDFETAGLRSNCTITGPSLRWVLDHGW
MMGDGTRWKSRWRGTLCCWRWMGRRCCA*DRSL
GP*PANAIPS*GLRLGGCSSPLPTFGCRWFLPW
MAACAGIPGWTNRPRSQHLPPLASEAVM*NQIPG
YFSLQGLRQNSISETFPSLMQSPGPSLWTWDSS
RQQAQATSLLLGHQRTHLGSVSTSKIKRRLFHL
FLPEWPLGTRSEAGCGPGPEQKP*DLDSQLPPEP
RQWH*RFPLKLHLRTPFES
Sequence SHBG.gAug05
ATTCAATCTCCGAGACATTCCCCAGCCTCATGCAGAGCCCTGGGCCTTCTCTTTGGACCTGGGACTCAAGCAGGCAGCAGGCTCAGGCCACCTCCTTGCTCTTGGGACACCAGAGAACCCATCTTGGCTCAGTCTCCACCTCCAAGATCAAGCCTGGCTCCCCTCCTTAACCTCTGGGCCAAGCCTCAAGGGCGTCTCTT
CCTGGGGGCTTTACCAGGAGAAGACTCTTCCACCTCTTTTTGCCTGAATGGCCTTTGGGCACAAGGTCAGAGGCTGGATGTGGACCAGGCCCTGAACAGA
AGCCATGAGATCTGGACTCACAGCTGCCCCCAGAGCCCAGGCAATGGCACTGACGCTTCCCATTAAAGCTCCACCTAAGAACCCCCTTTGAAAGTT
- SHBG.F2 (forward primer)
- SHBG.R2 (reverse primer)
- Single exon skipping
Frame 0
IQSPRHSPASCRALGLLFGPGTQAGSRLRPPPCSWDTREPILAQSPPPRSSLAPLLNLWAKPQGRLF
LGALPGEDSSTSFCLNGLWAQGQRLDVDQALNR
SHEIWTHSCPQSPGNGTDASH*SST*EPPLKV
Frame 1
FNLRDIPQPHAEPWAFSLDLGLKQAAGSGHLLALGTPENPSWLSLHLQDQAWLPSLTSGPSLKGVS
SWGLYQEKTLPPLFA*MAFGHKVRGWMWTRP*TE
AMRSGLTAAPRAQAMALTLPIKAPPKNPL*K
Frame 2
SISETFPSLMQSPGPSLWTWDSSRQQAQATSLLLGHQRTHLGSVSTSKIKPGSPP*PLGQASRASL
PGGFTRRRLFHLFLPEWPLGTRSEAGCGPGPEQ
KP*DLDSQLPPEPRQWH*RFPLKLHLRTPFES
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