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Sequences

Sequence SHBG.eAug05

CCGCGGTTCAAAGGCTCCCCCGCAGTGCTTTTTAAATTGACATATGCAGTGATAACCTGCTTTAGCCTCAGGCTCACTCACCCGCCCAGACCCTGGAGTG
CCCACGACCCTCCGGCTGTCCACCTCAGCAATGGCCCAGGACAAGAGCCTATCGCTGTCATGACCTTTGACCTCACCAAGATCACAAAAACCTCCTCCTC
CTTTGAGGTTCGAACCTGGGACCCAGAGGGAGTGATTTTTTATGGGGATACCAACCCTAAGGATGACTGGTTTATGCTGGGACTTCGAGACGGCAGGCCT
GAGATCCAACTGCACAATCACTGGGCCCAGCTTACGGTGGGTGCTGGACCACGGCTGGATGATGGGAGATGGCACCAGGTGGAAGTCAAGATGGAGGGGG
ACTCTGTGCTGCTGGAGGTGGATGGGGAGGAGGTGCTGCGCCTGAGACAGGTCTCTGGGCCCCTGACCAGCAAACGCCATCCCATCATGAGGATTGCGCT
TGGGGGGCTGCTCTTCCCCGCTTCCAACCTTCGGTTGCCGCTGGTTCCTGCCCTGGATGGCTGCCTGCGCCGGGATTCCTGGCTGGACAAACAGGCCGAG
ATCTCAGCATCTGCCCCCACTAGCCTCAGAAGCTGTGATGTAGAATCAAATCCCGGGATATTTCTCCCTCCAGGGACTCAGGCAGAATTCAATCTCCGAG
ACATTCCCCAGCCTCATGCAGAGCCCTGGGCCTTCTCTTTGGACCTGGGACTCAAGCAGGCAGCAGGCTCAGGCCACCTCCTTGCTCTTGGGACACCAGA
GAACCCATCTTGGCTCAGTCTCCACCTCCAAGATCAAGAGAAGACTCTTCCACCTCTTTTTGCCTGAATGGCCTTTGGGCACAAGGTCAGAGGCTGGATG
TGGACCAGGCCCTGAACAGAAGCCATGAGATCTGGACTCACAGCTGCCCCCAGAGCCCAGGCAATGGCACTGACGCTTCCCATTAAAGCTCCACCTAAGA
ACCCCCTTTGAAAGTT
  • SHBG.F2 (forward primer)
  • SHBG.R2 (reverse primer)
  • Single exon skipping

Frame 0

PRFKGSPAVLFKLTYAVITCFSLRLTHPPRPWS
AHDPPAVHLSNGPGQEPIAVMTFDLTKITKTSSS
FEVRTWDPEGVIFYGDTNPKDDWFMLGLRDGRP
EIQLHNHWAQLTVGAGPRLDDGRWHQVEVKMEG
DSVLLEVDGEEVLRLRQVSGPLTSKRHPIMRIAL
GGLLFPASNLRLPLVPALDGCLRRDSWLDKQAE
ISASAPTSLRSCDVESNPGIFLPPGTQAEFNLR
DIPQPHAEPWAFSLDLGLKQAAGSGHLLALGTPE
NPSWLSLHLQDQEKTLPPLFA*MAFGHKVRGWM
WTRP*TEAMRSGLTAAPRAQAMALTLPIKAPPK
NPL*K

Frame 1

RGSKAPPQCFLN*HMQ**PALASGSLTRPDPGV
PTTLRLSTSAMAQDKSLSLS*PLTSPRSQKPPP
PLRFEPGTQRE*FFMGIPTLRMTGLCWDFETAGL
RSNCTITGPSLRWVLDHGWMMGDGTRWKSRWRG
TLCCWRWMGRRCCA*DRSLGP*PANAIPS*GLR
LGGCSSPLPTFGCRWFLPWMAACAGIPGWTNRPR
SQHLPPLASEAVM*NQIPGYFSLQGLRQNSISE
TFPSLMQSPGPSLWTWDSSRQQAQATSLLLGHQ
RTHLGSVSTSKIKRRLFHLFLPEWPLGTRSEAGC
GPGPEQKP*DLDSQLPPEPRQWH*RFPLKLHLR
TPFES

Frame 2

AVQRLPRSAF*IDICSDNLL*PQAHSPAQTLEC
PRPSGCPPQQWPRTRAYRCHDL*PHQDHKNLLL
L*GSNLGPRGSDFLWGYQP*G*LVYAGTSRRQA
*DPTAQSLGPAYGGCWTTAG*WEMAPGGSQDGGG
LCAAGGGWGGGAAPETGLWAPDQQTPSHHEDCA
WGAALPRFQPSVAAGSCPGWLPAPGFLAGQTGR
DLSICPH*PQKL*CRIKSRDISPSRDSGRIQSPR
HSPASCRALGLLFGPGTQAGSRLRPPPCSWDTR
EPILAQSPPPRSREDSSTSFCLNGLWAQGQRLD
VDQALNRSHEIWTHSCPQSPGNGTDASH*SST*E
PPLKV

Sequence SHBG.dAug05

TGAGCCGCCGAGTGGACAGTGGCTGATTATGGAGAGCAGAGGCCCACTGGCTACCTCGCGCCTGCTGCTGTTGCTGCTGTTGCTACTACTGCGTCACACC
CGCCAGGGATGGGCCCTGAGACCTGTTCTCCCCACCCAGAGTGCCCACGACCCTCCGGCTGTCCACCTCAGCAATGGCCCAGGACAAGAGCCTATCGCTG
TCATGACCTTTGACCTCACCAAGATCACAAAAACCTCCTCCTCCTTTGAGGTTCGAACCTGGGACCCAGAGGGAGTGATTTTTTATGGGGATACCAACCC
TAAGGATGACTGGTTTATGCTGGGACTTCGAGACGGCAGGCCTGAGATCCAACTGCACAATCACTGGGCCCAGCTTACGGTGGGTGCTGGACCACGGCTG
GATGATGGGAGATGGCACCAGGTGGAAGTCAAGATGGAGGGGGACTCTGTGCTGCTGGAGGTGGATGGGGAGGAGGTGCTGCGCCTGAGACAGGTCTCTG
GGCCCCTGACCAGCAAACGCCATCCCATCATGAGGATTGCGCTTGGGGGGCTGCTCTTCCCCGCTTCCAACCTTCGGTTGCCGCTGGTTCCTGCCCTGGA
TGGCTGCCTGCGCCGGGATTCCTGGCTGGACAAACAGGCCGAGATCTCAGCATCTGCCCCCACTAGCCTCAGAAGCTGTGATGTAGAATCAAATCCCGGG
ATATTTCTCCCTCCAGGGACTCAGGCAGAATTCAATCTCCGAGACATTCCCCAGCCTCATGCAGAGCCCTGGGCCTTCTCTTTGGACCTGGGACTCAAGC
AGGCAGCAGGCTCAGGCCACCTCCTTGCTCTTGGGACACCAGAGAACCCATCTTGGCTCAGTCTCCACCTCCAAGATCAAGAGAAGACTCTTCCACCTCT
TTTTGCCTGAATGGCCTTTGGGCACAAGGTCAGAGGCTGGATGTGGACCAGGCCCTGAACAGAAGCCATGAGATCTGGACTCACAGCTGCCCCCAGAGCC
CAGGCAATGGCACTGACGCTTCCCATTAAAGCTCCACCTAAGAACCCCCTTTGAAAGTT
  • SHBG.F2 (forward primer)
  • SHBG.R2 (reverse primer)
  • Single exon skipping

Frame 0

*AAEWTVADYGEQRPTGYLAPAAVAAVATTASH
PPGMGPETCSPHPECPRPSGCPPQQWPRTRAYRC
HDL*PHQDHKNLLLL*GSNLGPRGSDFLWGYQP
*G*LVYAGTSRRQA*DPTAQSLGPAYGGCWTTA
G*WEMAPGGSQDGGGLCAAGGGWGGGAAPETGLW
APDQQTPSHHEDCAWGAALPRFQPSVAAGSCPG
WLPAPGFLAGQTGRDLSICPH*PQKL*CRIKSR
DISPSRDSGRIQSPRHSPASCRALGLLFGPGTQA
GSRLRPPPCSWDTREPILAQSPPPRSREDSSTS
FCLNGLWAQGQRLDVDQALNRSHEIWTHSCPQS
PGNGTDASH*SST*EPPLKV

Frame 1

EPPSGQWLIMESRGPLATSRLLLLLLLLLLRHT
RQGWALRPVLPTQSAHDPPAVHLSNGPGQEPIA
VMTFDLTKITKTSSSFEVRTWDPEGVIFYGDTNP
KDDWFMLGLRDGRPEIQLHNHWAQLTVGAGPRL
DDGRWHQVEVKMEGDSVLLEVDGEEVLRLRQVS
GPLTSKRHPIMRIALGGLLFPASNLRLPLVPALD
GCLRRDSWLDKQAEISASAPTSLRSCDVESNPG
IFLPPGTQAEFNLRDIPQPHAEPWAFSLDLGLK
QAAGSGHLLALGTPENPSWLSLHLQDQEKTLPPL
FA*MAFGHKVRGWMWTRP*TEAMRSGLTAAPRA
QAMALTLPIKAPPKNPL*K

Frame 2

SRRVDSG*LWRAEAHWLPRACCCCCCCYYCVTP
ARDGP*DLFSPPRVPTTLRLSTSAMAQDKSLSL
S*PLTSPRSQKPPPPLRFEPGTQRE*FFMGIPT
LRMTGLCWDFETAGLRSNCTITGPSLRWVLDHGW
MMGDGTRWKSRWRGTLCCWRWMGRRCCA*DRSL
GP*PANAIPS*GLRLGGCSSPLPTFGCRWFLPW
MAACAGIPGWTNRPRSQHLPPLASEAVM*NQIPG
YFSLQGLRQNSISETFPSLMQSPGPSLWTWDSS
RQQAQATSLLLGHQRTHLGSVSTSKIKRRLFHL
FLPEWPLGTRSEAGCGPGPEQKP*DLDSQLPPEP
RQWH*RFPLKLHLRTPFES

Sequence SHBG.fAug05

TGAGCCGCCGAGTGGACAGTGGCTGATTATGGAGAGCAGAGGCCCACTGGCTACCTCGCGCCTGCTGCTGTTGCTGCTGTTGCTACTACTGCGTCACACC
CGCCAGGGATGGGCCCTGAGACCTGTTCTCCCCACCCAGAGTGCCCACGACCCTCCGGCTGTCCACCTCAGCAATGGCCCAGGACAAGAGCCTATCGCTG
TCATGACCTTTGACCTCACCAAGATCACAAAAACCTCCTCCTCCTTTGAGGTTCGAACCTGGGACCCAGAGGGAGTGATTTTTTATGGGGATACCAACCC
TAAGGATGACTGGTTTATGCTGGGACTTCGAGACGGCAGGCCTGAGATCCAACTGCACAATCACTGGGCCCAGCTTACGGTGGGTGCTGCGCCTGAGACA
GGTCTCTGGGCCCCTGACCAGCAAACGCCATCCCATCATGAGGATTGCGCTTGGGGGGCTGCTCTTCCCCGCTTCCAACCTTCGGTTGCCGCTGGTTCCT
GCCCTGGATGGCTGCCTGCGCCGGGATTCCTGGCTGGACAAACAGGCCGAGATCTCAGCATCTGCCCCCACTAGCCTCAGAAGCTGTGATGTAGAATCAA
ATCCCGGGATATTTCTCCCTCCAGGGACTCAGGCAGAATTCAATCTCCGAGACATTCCCCAGCCTCATGCAGAGCCCTGGGCCTTCTCTTTGGACCTGGG
ACTCAAGCAGGCAGCAGGCTCAGGCCACCTCCTTGCTCTTGGGACACCAGAGAACCCATCTTGGCTCAGTCTCCACCTCCAAGATCAAAAGGTGGTGTTG
TCTTCTGGGTCGGGGCCAGGGCTGGATCTGCCCCTGGTCTTGGGACTCCCTCTTCAGCTGAAGCTGAGTATGTCCAGGGTGGTCTTGAGCCAAGGGTCGA
AGATGAAGGCCCTTGCCCTGCCTCCCTTAGGCCTGGCTCCCCTCCTTAACCTCTGGGCCAAGCCTCAAGGGCGTCTCTTCCTGGGGGCTTTACCAGGAGA
AGACTCTTCCACCTCTTTTTGCCTGAATGGCCTTTGGGCACAAGGTCAGAGGCTGGATGTGGACCAGGCCCTGAACAGAAGCCATGAGATCTGGACTCAC
AGCTGCCCCCAGAGCCCAGGCAATGGCACTGACGCTTCCCATTAAAGCTCCACCTAAGAACCCCCTTTGAAAGTT
  • SHBG.F2 (forward primer)
  • SHBG.R2 (reverse primer)
  • Single exon skipping

Frame 0

*AAEWTVADYGEQRPTGYLAPAAVAAVATTASH
PPGMGPETCSPHPECPRPSGCPPQQWPRTRAYRC
HDL*PHQDHKNLLLL*GSNLGPRGSDFLWGYQP
*G*LVYAGTSRRQA*DPTAQSLGPAYGGCCA*D
RSLGP*PANAIPS*GLRLGGCSSPLPTFGCRWFL
PWMAACAGIPGWTNRPRSQHLPPLASEAVM*NQ
IPGYFSLQGLRQNSISETFPSLMQSPGPSLWTW
DSSRQQAQATSLLLGHQRTHLGSVSTSKIKRWCC
LLGRGQGWICPWSWDSLFS*S*VCPGWS*AKGR
R*RPLPCLP*AWLPSLTSGPSLKGVSSWGLYQE
KTLPPLFA*MAFGHKVRGWMWTRP*TEAMRSGLT
AAPRAQAMALTLPIKAPPKNPL*K

Frame 1

EPPSGQWLIMESRGPLATSRLLLLLLLLLLRHT
RQGWALRPVLPTQSAHDPPAVHLSNGPGQEPIA
VMTFDLTKITKTSSSFEVRTWDPEGVIFYGDTNP
KDDWFMLGLRDGRPEIQLHNHWAQLTVGAAPET
GLWAPDQQTPSHHEDCAWGAALPRFQPSVAAGS
CPGWLPAPGFLAGQTGRDLSICPH*PQKL*CRIK
SRDISPSRDSGRIQSPRHSPASCRALGLLFGPG
TQAGSRLRPPPCSWDTREPILAQSPPPRSKGGV
VFWVGARAGSAPGLGTPSSAEAEYVQGGLEPRVE
DEGPCPASLRPGSPP*PLGQASRASLPGGFTRR
RLFHLFLPEWPLGTRSEAGCGPGPEQKP*DLDS
QLPPEPRQWH*RFPLKLHLRTPFES

Frame 2

SRRVDSG*LWRAEAHWLPRACCCCCCCYYCVTP
ARDGP*DLFSPPRVPTTLRLSTSAMAQDKSLSL
S*PLTSPRSQKPPPPLRFEPGTQRE*FFMGIPT
LRMTGLCWDFETAGLRSNCTITGPSLRWVLRLRQ
VSGPLTSKRHPIMRIALGGLLFPASNLRLPLVP
ALDGCLRRDSWLDKQAEISASAPTSLRSCDVES
NPGIFLPPGTQAEFNLRDIPQPHAEPWAFSLDLG
LKQAAGSGHLLALGTPENPSWLSLHLQDQKVVL
SSGSGPGLDLPLVLGLPLQLKLSMSRVVLSQGS
KMKALALPPLGLAPLLNLWAKPQGRLFLGALPGE
DSSTSFCLNGLWAQGQRLDVDQALNRSHEIWTH
SCPQSPGNGTDASH*SST*EPPLKV

Sequence SHBG.cAug05

CCGAGTGGACAGTGGCTGATTATGGAGAGCAGAGGCCCACTGGCTACCTCGCGCCTGCTGCTGTTGCTGCTGTTGCTACTACTGCGTCACACCCGCCAGG
GATGGGCCCTGAGACCTGTTCTCCCCACCCAGGTGCAGGAGCGGGACAGGGCACTCAGCTCATGCAGTCTTCCCTTCTCTCCTCTGGCCCTGTAGCAGGG
CCTCTCCCTCTGTCTGTCTCTGACATGTCCCTACTCAGCTTTGTTTGTTTTCTCTTTCTGATAGAGTGCCCACGACCCTCCGGCTGTCCACCTCAGCAAT
GGCCCAGGACAAGAGCCTATCGCTGTCATGACCTTTGACCTCACCAAGATCACAAAAACCTCCTCCTCCTTTGAGGTTCGAACCTGGGACCCAGAGGGAG
TGATTTTTTATGGGGATACCAACCCTAAGGATGACTGGTTTATGCTGGGACTTCGAGACGGCAGGCCTGAGATCCAACTGCACAATCACTGGGCCCAGCT
TACGGTGGGTGCTGGACCACGGCTGGATGATGGGAGATGGCACCAGGTGGAAGTCAAGATGGAGGGGGACTCTGTGCTGCTGGAGGTGGATGGGGAGGAG
GTGCTGCGCCTGAGACAGGTCTCTGGGCCCCTGACCAGCAAACGCCATCCCATCATGAGGATTGCGCTTGGGGGGCTGCTCTTCCCCGCTTCCAACCTTC
GGTTGCCGCTGGTTCCTGCCCTGGATGGCTGCCTGCGCCGGGATTCCTGGCTGGACAAACAGGCCGAGATCTCAGCATCTGCCCCCACTAGCCTCAGAAG
CTGTGATGTAGAATCAAATCCCGGGATATTTCTCCCTCCAGGGACTCAGGCAGAATTCAATCTCCGAGACATTCCCCAGCCTCATGCAGAGCCCTGGGCC
TTCTCTTTGGACCTGGGACTCAAGCAGGCAGCAGGCTCAGGCCACCTCCTTGCTCTTGGGACACCAGAGAACCCATCTTGGCTCAGTCTCCACCTCCAAG
ATCAAAAGGTGGTGTTGTCTTCTGGGTCGGGGCCAGGGCTGGATCTGCCCCTGGTCTTGGGACTCCCTCTTCAGCTGAAGCTGAGTATGTCCAGGGTGGT
CTTGAGCCAAGGGTCGAAGATGAAGGCCCTTGCCCTGCCTCCCTTAGGCCTGGCTCCCCTCCTTAACCTCTGGGCCAAGCCTCAAGGGCGTCTCTTCCTG
GGGGCTTTACCAGGAGAAGACTCTTCCACCTCTTTTTGCCTGAATGGCCTTTGGGCACAAGGTCAGAGGCTGGATGTGGACCAGGCCCTGAACAGAAGCC
ATGAGATCTGGACTCACAGCTGCCCCCAGAGCCCAGGCAATGGCACTGACGCTTCCCATTAAAGCTCCACCTAAGAACCCCCTTTGAAAGTT
  • SHBG.F2 (forward primer)
  • SHBG.R2 (reverse primer)
  • Single exon skipping

Frame 0

PSGQWLIMESRGPLATSRLLLLLLLLLLRHTRQ
GWALRPVLPTQVQERDRALSSCSLPFSPLAL*QG
LSLCLSLTCPYSALFVFSF**SAHDPPAVHLSN
GPGQEPIAVMTFDLTKITKTSSSFEVRTWDPEG
VIFYGDTNPKDDWFMLGLRDGRPEIQLHNHWAQL
TVGAGPRLDDGRWHQVEVKMEGDSVLLEVDGEE
VLRLRQVSGPLTSKRHPIMRIALGGLLFPASNL
RLPLVPALDGCLRRDSWLDKQAEISASAPTSLRS
CDVESNPGIFLPPGTQAEFNLRDIPQPHAEPWA
FSLDLGLKQAAGSGHLLALGTPENPSWLSLHLQ
DQKVVLSSGSGPGLDLPLVLGLPLQLKLSMSRVV
LSQGSKMKALALPPLGLAPLLNLWAKPQGRLFL
GALPGEDSSTSFCLNGLWAQGQRLDVDQALNRS
HEIWTHSCPQSPGNGTDASH*SST*EPPLKV

Frame 1

RVDSG*LWRAEAHWLPRACCCCCCCYYCVTPAR
DGP*DLFSPPRCRSGTGHSAHAVFPSLLWPCSR
ASPSVCL*HVPTQLCLFSLSDRVPTTLRLSTSAM
AQDKSLSLS*PLTSPRSQKPPPPLRFEPGTQRE
*FFMGIPTLRMTGLCWDFETAGLRSNCTITGPS
LRWVLDHGWMMGDGTRWKSRWRGTLCCWRWMGRR
CCA*DRSLGP*PANAIPS*GLRLGGCSSPLPTF
GCRWFLPWMAACAGIPGWTNRPRSQHLPPLASE
AVM*NQIPGYFSLQGLRQNSISETFPSLMQSPGP
SLWTWDSSRQQAQATSLLLGHQRTHLGSVSTSK
IKRWCCLLGRGQGWICPWSWDSLFS*S*VCPGW
S*AKGRR*RPLPCLP*AWLPSLTSGPSLKGVSSW
GLYQEKTLPPLFA*MAFGHKVRGWMWTRP*TEA
MRSGLTAAPRAQAMALTLPIKAPPKNPL*K

Frame 2

EWTVADYGEQRPTGYLAPAAVAAVATTASHPPG
MGPETCSPHPGAGAGQGTQLMQSSLLSSGPVAG
PLPLSVSDMSLLSFVCFLFLIECPRPSGCPPQQ
WPRTRAYRCHDL*PHQDHKNLLLL*GSNLGPRGS
DFLWGYQP*G*LVYAGTSRRQA*DPTAQSLGPA
YGGCWTTAG*WEMAPGGSQDGGGLCAAGGGWGG
GAAPETGLWAPDQQTPSHHEDCAWGAALPRFQPS
VAAGSCPGWLPAPGFLAGQTGRDLSICPH*PQK
L*CRIKSRDISPSRDSGRIQSPRHSPASCRALG
LLFGPGTQAGSRLRPPPCSWDTREPILAQSPPPR
SKGGVVFWVGARAGSAPGLGTPSSAEAEYVQGG
LEPRVEDEGPCPASLRPGSPP*PLGQASRASLP
GGFTRRRLFHLFLPEWPLGTRSEAGCGPGPEQKP
*DLDSQLPPEPRQWH*RFPLKLHLRTPFES

Sequence SHBG.bAug05

TGAGCCGCCGAGTGGACAGTGGCTGATTATGGAGAGCAGAGGCCCACTGGCTACCTCGCGCCTGCTGCTGTTGCTGCTGTTGCTACTACTGCGTCACACC
CGCCAGGGATGGGCCCTGAGACCTGTTCTCCCCACCCAGAGTGCCCACGACCCTCCGGCTGTCCACCTCAGCAATGGCCCAGGACAAGAGCCTATCGCTG
TCATGACCTTTGACCTCACCAAGATCACAAAAACCTCCTCCTCCTTTGAGGTTCGAACCTGGGACCCAGAGGGAGTGATTTTTTATGGGGATACCAACCC
TAAGGATGACTGGTTTATGCTGGGACTTCGAGACGGCAGGCCTGAGATCCAACTGCACAATCACTGGGCCCAGCTTACGGTGGGTGCTGGACCACGGCTG
GATGATGGGAGATGGCACCAGGTGGAAGTCAAGATGGAGGGGGACTCTGTGCTGCTGGAGGTGGATGGGGAGGAGGTGCTGCGCCTGAGACAGGTCTCTG
GGCCCCTGACCAGCAAACGCCATCCCATCATGAGGATTGCGCTTGGGGGGCTGCTCTTCCCCGCTTCCAACCTTCGGTTGCCGGCCGAGATCTCAGCATC
TGCCCCCACTAGCCTCAGAAGCTGTGATGTAGAATCAAATCCCGGGATATTTCTCCCTCCAGGGACTCAGGCAGAATTCAATCTCCGAGACATTCCCCAG
CCTCATGCAGAGCCCTGGGCCTTCTCTTTGGACCTGGGACTCAAGCAGGCAGCAGGCTCAGGCCACCTCCTTGCTCTTGGGACACCAGAGAACCCATCTT
GGCTCAGTCTCCACCTCCAAGATCAAAAGGTGGTGTTGTCTTCTGGGTCGGGGCCAGGGCTGGATCTGCCCCTGGTCTTGGGACTCCCTCTTCAGCTGAA
GCTGAGTATGTCCAGGGTGGTCTTGAGCCAAGGGTCGAAGATGAAGGCCCTTGCCCTGCCTCCCTTAGGCCTGGCTCCCCTCCTTAACCTCTGGGCCAAG
CCTCAAGGGCGTCTCTTCCTGGGGGCTTTACCAGGAGAAGACTCTTCCACCTCTTTTTGCCTGAATGGCCTTTGGGCACAAGGTCAGAGGCTGGATGTGG
ACCAGGCCCTGAACAGAAGCCATGAGATCTGGACTCACAGCTGCCCCCAGAGCCCAGGCAATGGCACTGACGCTTCCCATTAAAGCTCCACCTAAGAACC
CCCTTTGAAAGTT
  • SHBG.F2 (forward primer)
  • SHBG.R2 (reverse primer)
  • Single exon skipping

Frame 0

*AAEWTVADYGEQRPTGYLAPAAVAAVATTASH
PPGMGPETCSPHPECPRPSGCPPQQWPRTRAYRC
HDL*PHQDHKNLLLL*GSNLGPRGSDFLWGYQP
*G*LVYAGTSRRQA*DPTAQSLGPAYGGCWTTA
G*WEMAPGGSQDGGGLCAAGGGWGGGAAPETGLW
APDQQTPSHHEDCAWGAALPRFQPSVAGRDLSI
CPH*PQKL*CRIKSRDISPSRDSGRIQSPRHSP
ASCRALGLLFGPGTQAGSRLRPPPCSWDTREPIL
AQSPPPRSKGGVVFWVGARAGSAPGLGTPSSAE
AEYVQGGLEPRVEDEGPCPASLRPGSPP*PLGQ
ASRASLPGGFTRRRLFHLFLPEWPLGTRSEAGCG
PGPEQKP*DLDSQLPPEPRQWH*RFPLKLHLRT
PFES

Frame 1

EPPSGQWLIMESRGPLATSRLLLLLLLLLLRHT
RQGWALRPVLPTQSAHDPPAVHLSNGPGQEPIA
VMTFDLTKITKTSSSFEVRTWDPEGVIFYGDTNP
KDDWFMLGLRDGRPEIQLHNHWAQLTVGAGPRL
DDGRWHQVEVKMEGDSVLLEVDGEEVLRLRQVS
GPLTSKRHPIMRIALGGLLFPASNLRLPAEISAS
APTSLRSCDVESNPGIFLPPGTQAEFNLRDIPQ
PHAEPWAFSLDLGLKQAAGSGHLLALGTPENPS
WLSLHLQDQKVVLSSGSGPGLDLPLVLGLPLQLK
LSMSRVVLSQGSKMKALALPPLGLAPLLNLWAK
PQGRLFLGALPGEDSSTSFCLNGLWAQGQRLDV
DQALNRSHEIWTHSCPQSPGNGTDASH*SST*EP
PLKV

Frame 2

SRRVDSG*LWRAEAHWLPRACCCCCCCYYCVTP
ARDGP*DLFSPPRVPTTLRLSTSAMAQDKSLSL
S*PLTSPRSQKPPPPLRFEPGTQRE*FFMGIPT
LRMTGLCWDFETAGLRSNCTITGPSLRWVLDHGW
MMGDGTRWKSRWRGTLCCWRWMGRRCCA*DRSL
GP*PANAIPS*GLRLGGCSSPLPTFGCRPRSQH
LPPLASEAVM*NQIPGYFSLQGLRQNSISETFPS
LMQSPGPSLWTWDSSRQQAQATSLLLGHQRTHL
GSVSTSKIKRWCCLLGRGQGWICPWSWDSLFS*
S*VCPGWS*AKGRR*RPLPCLP*AWLPSLTSGPS
LKGVSSWGLYQEKTLPPLFA*MAFGHKVRGWMW
TRP*TEAMRSGLTAAPRAQAMALTLPIKAPPKN
PL*K

Sequence SHBG.aAug05

TGAGCCGCCGAGTGGACAGTGGCTGATTATGGAGAGCAGAGGCCCACTGGCTACCTCGCGCCTGCTGCTGTTGCTGCTGTTGCTACTACTGCGTCACACC
CGCCAGGGATGGGCCCTGAGACCTGTTCTCCCCACCCAGAGTGCCCACGACCCTCCGGCTGTCCACCTCAGCAATGGCCCAGGACAAGAGCCTATCGCTG
TCATGACCTTTGACCTCACCAAGATCACAAAAACCTCCTCCTCCTTTGAGGTTCGAACCTGGGACCCAGAGGGAGTGATTTTTTATGGGGATACCAACCC
TAAGGATGACTGGTTTATGCTGGGACTTCGAGACGGCAGGCCTGAGATCCAACTGCACAATCACTGGGCCCAGCTTACGGTGGGTGCTGGACCACGGCTG
GATGATGGGAGATGGCACCAGGTGGAAGTCAAGATGGAGGGGGACTCTGTGCTGCTGGAGGTGGATGGGGAGGAGGTGCTGCGCCTGAGACAGGTCTCTG
GGCCCCTGACCAGCAAACGCCATCCCATCATGAGGATTGCGCTTGGGGGGCTGCTCTTCCCCGCTTCCAACCTTCGGTTGCCGCTGGTTCCTGCCCTGGA
TGGCTGCCTGCGCCGGGATTCCTGGCTGGACAAACAGGCCGAGATCTCAGCATCTGCCCCCACTAGCCTCAGAAGCTGTGATGTAGAATCAAATCCCGGG
ATATTTCTCCCTCCAGGGACTCAGGCAGAATTCAATCTCCGAGACATTCCCCAGCCTCATGCAGAGCCCTGGGCCTTCTCTTTGGACCTGGGACTCAAGC
AGGCAGCAGGCTCAGGCCACCTCCTTGCTCTTGGGACACCAGAGAACCCATCTTGGCTCAGTCTCCACCTCCAAGATCAAAAGGTGGTGTTGTCTTCTGG
GTCGGGGCCAGGGCTGGATCTGCCCCTGGTCTTGGGACTCCCTCTTCAGCTGAAGCTGAGTATGTCCAGGGTGGTCTTGAGCCAAGGGTCGAAGATGAAG
GCCCTTGCCCTGCCTCCCTTAGGCCTGGCTCCCCTCCTTAACCTCTGGGCCAAGCCTCAAGGGCGTCTCTTCCTGGGGGCTTTACCAGGAGAAGACTCTT
CCACCTCTTTTTGCCTGAATGGCCTTTGGGCACAAGGTCAGAGGCTGGATGTGGACCAGGCCCTGAACAGAAGCCATGAGATCTGGACTCACAGCTGCCC
CCAGAGCCCAGGCAATGGCACTGACGCTTCCCATTAAAGCTCCACCTAAGAACCCCCTTTGAAAGTT
  • SHBG.F2 (forward primer)
  • SHBG.R2 (reverse primer)
  • Single exon skipping

Frame 0

*AAEWTVADYGEQRPTGYLAPAAVAAVATTASH
PPGMGPETCSPHPECPRPSGCPPQQWPRTRAYRC
HDL*PHQDHKNLLLL*GSNLGPRGSDFLWGYQP
*G*LVYAGTSRRQA*DPTAQSLGPAYGGCWTTA
G*WEMAPGGSQDGGGLCAAGGGWGGGAAPETGLW
APDQQTPSHHEDCAWGAALPRFQPSVAAGSCPG
WLPAPGFLAGQTGRDLSICPH*PQKL*CRIKSR
DISPSRDSGRIQSPRHSPASCRALGLLFGPGTQA
GSRLRPPPCSWDTREPILAQSPPPRSKGGVVFW
VGARAGSAPGLGTPSSAEAEYVQGGLEPRVEDE
GPCPASLRPGSPP*PLGQASRASLPGGFTRRRLF
HLFLPEWPLGTRSEAGCGPGPEQKP*DLDSQLP
PEPRQWH*RFPLKLHLRTPFES

Frame 1

EPPSGQWLIMESRGPLATSRLLLLLLLLLLRHT
RQGWALRPVLPTQSAHDPPAVHLSNGPGQEPIA
VMTFDLTKITKTSSSFEVRTWDPEGVIFYGDTNP
KDDWFMLGLRDGRPEIQLHNHWAQLTVGAGPRL
DDGRWHQVEVKMEGDSVLLEVDGEEVLRLRQVS
GPLTSKRHPIMRIALGGLLFPASNLRLPLVPALD
GCLRRDSWLDKQAEISASAPTSLRSCDVESNPG
IFLPPGTQAEFNLRDIPQPHAEPWAFSLDLGLK
QAAGSGHLLALGTPENPSWLSLHLQDQKVVLSSG
SGPGLDLPLVLGLPLQLKLSMSRVVLSQGSKMK
ALALPPLGLAPLLNLWAKPQGRLFLGALPGEDS
STSFCLNGLWAQGQRLDVDQALNRSHEIWTHSCP
QSPGNGTDASH*SST*EPPLKV

Frame 2

SRRVDSG*LWRAEAHWLPRACCCCCCCYYCVTP
ARDGP*DLFSPPRVPTTLRLSTSAMAQDKSLSL
S*PLTSPRSQKPPPPLRFEPGTQRE*FFMGIPT
LRMTGLCWDFETAGLRSNCTITGPSLRWVLDHGW
MMGDGTRWKSRWRGTLCCWRWMGRRCCA*DRSL
GP*PANAIPS*GLRLGGCSSPLPTFGCRWFLPW
MAACAGIPGWTNRPRSQHLPPLASEAVM*NQIPG
YFSLQGLRQNSISETFPSLMQSPGPSLWTWDSS
RQQAQATSLLLGHQRTHLGSVSTSKIKRWCCLL
GRGQGWICPWSWDSLFS*S*VCPGWS*AKGRR*R
PLPCLP*AWLPSLTSGPSLKGVSSWGLYQEKTL
PPLFA*MAFGHKVRGWMWTRP*TEAMRSGLTAA
PRAQAMALTLPIKAPPKNPL*K

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