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Sequences

Sequence ECT2.hAug05

ECT2.F11 (forward primer)
ECT2.R10 (reverse primer)
Alternative donor

Frame 0

RLTSRANRHGS*REQLSF*IGCGGRRRGMAVFV RGVGV*RGGTPRAFLRVTESTSCYLDWSAVARSR LTATSASRVQAILLPQPPE*LGLQVPATKPS*F LYF**RRGFIVLARLVSNS*PRDPPALASQSAR ITSVSHRVRPFKC*FRRIQIMAENSVLTSTTGRT SLADSSIFDSKVTEISKENLLIGSTSYVEEEMP QIETRVILVQEAGKQEELIKALKTIKIMEVPVI KIKESCPGKSDEKLIKSVINMDIKVGFVKMESVE EFEGLDSPEFENVFVVTDFQDSVFNDLYKADCR VIGPPVVLNC

Frame 1

G*HHAPIGMALRESSLVFESVVAAAGEEWRYL* EESAFEEVELLGLF*E*RSLPLVT*TGVQWHDL GSLQPLPPGFKRFSCLSLLSSWDYRCLPPSPANF CIFSRDGVSLCWPGWSRTPDLVIRPPWPPKVLG LQV*ATASGLSNADLEEYKSWLKIVY*HPLLGG LAWQTLPFLILKLLRFPRKTYLLDLLHM*KKRCL RLKQE*YWFKKLENKKNL*KP*RLLK*WKSLL* R*KKVVLENRMKN**KVLLIWTLKWAL*RWSQW KNLKVWILRNLKMYL*SRTFRILSLMTSTRLIVE LLDHQLY*I

Frame 2

VDITRQSAWLLERAA*FLNRLWRPPARNGGICE RSRRLKRWNS*GFFESDGVYLLLPRLECSGTIS AHCNLCLPGSSDSPASAS*VAGITGACHQAQLI FVFLVETGFHCVGQAGLELLTS*SARLGLPKC*D YKCEPPRPAFQMLI*KNTNHG*K*CINIHYWED *LGRLFHF*F*SY*DFQGKLTYWIYFICRRRDA SD*NKSDIGSRSWKTRRTYKSLKDY*NNGSPCYK DKRKLSWKIG*KINKKCY*YGH*SGLCKDGVSG RI*RFGFSGI*KCICSHGLSGFCL**PLQG*L* SYWTTSCIKL

Sequence ECT2.bAug05

AGGTTGACATCACGCGCCAATCGGCATGGCTCTTAGAGAGAGCAGCTTAGTTTTTGAATCGGTTGTGGCGGCCGCCGGCGAGGAATGGCGGTATTTGTGA GAGGAGTCGGCGTTTGAAGAGGTGGAACTCCTAGGGCTTTTTTGAGAGTGACGGAGTCTACCTCTTGTTACCTAGACTGGAGTGCAGTGGCACGATCTCG GCTCACTGCAACCTCTGCCTCCCGGGTTCAAGCGATTCTCCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGTGCCTGCCACCAAGCCCAGCTAATTT TTGTATTTTTAGTAGAGACGGGGTTTCATTGTGTTGGCCAGGCTGGTCTCGAACTCCTGACCTCGTGATCCGCCCGCCTTGGCCTCCCAAAGTGCTAGGA TTACAAGTGTGAGCCACCGCGTCCGGCCTTTCAAATGGTATTTTTGATTTTCCTCTTCCAGTCCTTAAAGCAGCTGATTTAGAAGAATACAAATCATGGC TGAAAATAGTGTATTAACATCCACTACTGGGAGGACTAGCTTGGCAGACTCTTCCATTTTTGATTCTAAAGTTACTGAGATTTCCAAGGAAAACTTACTT ATTGGATCTACTTCATATGTAGAAGAGATGCCTCAGATTGAAACAAGAGTGATATTGGTTCAAGAAGCTGGAAAACAAGAAGAACTTATAAAAGCCTTAA AGGACATTAAAGTGGGCTTTGTAAAGATGGAGTCAGTGGAAGAATTTGAAGGTTTGGATTCTCCGGAATTTGAAAATGTATTTGTAGTCACGGACTTTCA GGATTCTGTCTTTAATGACCTCTACAAGGCTGATTGTAGAGTTATTGGACCACCAGTTGTATTAAATTGTTCACAAAAAGGAGAGCCTTTGCCATTTTCA TGTCGCCCGTTGTATTGTACAAGTATGATGAATCTAGTACTATGCTTTACTGGATTTAGGAAAAAAGAAGAACTAGTCAGGTTGGTGACATTGGTCCATC ACATGGGTGGAGTTATTCGAAAAGACTTTAATTCAAAAGTTACACATTTGGTGGCAAATTGTACACAAGGAGAAAAATTCAGGGTTGCTGTGAGTCTAGG TACTCCAATTATGAAGCCAGAATGGATTTATAAAGCTTGGGAAAGGCGGAATGAACAGGATTTCTATGCAGCAGTTGATGACTTTAGAAATGAATTTAAA GTTCCTCCATTTCAAGATTGTATTTTAAGTTTCCTGGGATTTTCAGATGAAGAGAAAACCAATATGGAAGAAATGACTGAAATGCAAGGAGGTAAATATT TACCGCTTGGAGATGAAAGATGCACTCACCTTGTAGTTGAAGAGAATATAGTAAAAGATCTTCCCTTTGAACCTTCAAAGAAACTTTATGTTGTCAAGCA AGAGTGGTTCTGGGGAAGCATTCAAATGGATGCCCGAGCTGGAGAAACTATGTATTTATATGAAAAGGCAAATACTCCTGAGCTCAAGAAATCAGTGTCA ATGCTTTCTCTAAATACCCCTAACAGCAATCGCAAACGACGTCGTTTAAAAGAAACACTTGCTCAGCTTTCAAGAGAGACAGACGTGTCACCATTTCCAC CCCGTAAGCGCCCATCAGCTGAGCATTCCCTTTCCATAGGGTCACTCCTAGATATCTCCAACACACCAGAGTCTAGCATTAACTATGGAGACACCCCAAA GTCTTGTACTAAGTCTTCTAAAAGCTCCACTCCAGTTCCTTCAAAGCAGTCAGCAAGGTGGCAAGTTGCAAAAGAGCTTTATCAAACTGAAAGTAATTAT GTTAATATATTGGCAACAATTATTCAGTTATTTCAAGTACCATTGGAAGAGGAAGGACAACGTGGTGGACCTATCCTTGCACCAGAGGAGATTAAGACTA TTTTTGGTAGCATCCCAGATATCTTTGATGTACACACTAAGATAAAGGATGATCTTGAAGACCTTATAGTTAATTGGGATGAGAGCAAAAGCATTGGTGA CATTTTTCTGAAATATTCAAAAGATTTGGTAAAAACCTACCCTCCCTTTGTAAACTTCTTTGAAATGAGCAAGGAAACAATTATTAAATGTGAAAAACAG AAACCAAGATTTCATGCTTTTCTCAAGATAAACCAAGCAAAACCAGAATGTGGACGGCAGAGCCTTGTTGAACTTCTTATCCGACCAGTACAGAGGTTAC CCAGTGTTGCATTACTTTTAAATGATCTTAAGAAGCATACAGCTGATGAAAATCCAGACAAAAGCACTTTAGAAAAAGCTATTGGATCACTGAAGGAAGT AATGACGCATATTAATGAGGATAAGAGAAAAACAGAAGCTCAAAAGCAAATTTTTGATGTTGTTTATGAAGTAGATGGATGCCCAGCTAATCTTTTATCT TCTCACCGAAGCTTAGTACAGCGGGTTGAAACAATTTCTCTAGGTGAGCACCCCTGTGACAGAGGAGAACAAGTAACTCTCTTCCTCTTCAATGATTGCC TAGAGATAGCAAGAAAACGGCACAAGGTTATTGGCACTTTTAGGAGTCCTCATGGCCAAACCCGACCCCCAGCTTCTCTTAAGCATATTCACCTAATGCC TCTTTCTCAGATTAAGAAGGTATTGGACATAAGAGAGACAGAAGATTGCCATAATGCTTTTGCCTTGCTTGTGAGGCCACCAACAGAGCAGGCAAATGTG CTACTCAGTTTCCAGATGACATCAGATGAACTTCCAAAAGAAAACTGGCTAAAGATGCTGTGTCGACATGTAGCTAACACCATTTGTAAAGCAGATGCTG AGAATCTTATTTATACTGCTGATCCAGAATCCTTTGAAGTAAATACAAAAGATATGGACAGTACATTGAGTAGAGCATCAAGAGCAATAAAAAAGACTTC AAAAAAGGTTACAAGAGCATTCTCTTTCTCCAAAACTCCAAAAAGAGCTCTTCGAAGGGCTCTTATGACATCCCACGGCTCAGTGGAGGGAAGAAGTCCT TCCAGCAATGATAAGCATGTAATGAGTCGTCTTTCTAGCACATCATCATTAGCAGGTATCCCTTCTCCCTCCCTTGTCAGCCTTCCTTCCTTCTTTGAAA GGAGAAGTCATACGTTAAGTAGATCTACAACTCATTTGATATGAAGCGTTACCAAAATCTTAAATTATAGAAATGTATAGACACCTCATACTCAAATAAG AAACTGACTTAAATGGTACTTGTAATTAGCACTTGGTGAAAGCTGGAAGGAAGATAAATAACACTAAACTATGCTATTTGATTTTTCTTCTTGAAAGAGT AAGGTTTACCTGTTACATTTTCAAGTTAATTCATGTAAAAAATGATAGTGATTTTGATGTAATTTATCTCTTGTTTGAATCTGTCATTCAAAGGCCAATA ATTTAAGTTGCTATCAGCTGATATTAGTAGCTTTGCAACCCTGATAGAGTAAATAAATTTTATGGGCGGGTGCCAAATACTGCTGTGAATCTATTTGTAT AGTATCCATGAATGAATTTATGGAAATAGATATTTGTGCAGCTCAATTTATGCAGAGATTAAATGACATCATAATACTGGATGAAAACTTGCATAGAATT CTGATTAAATAGTGGGTCTGTTTCACATGTGCAGTTTGAAGTATTTAAATAACCACTCCTTTCACAGTTTATTTTCTTCTCAAGCGTTTTCAAGATCTAG CATGTGGATTTTAAAAGATTTGCCCTCATTAACAAGAATAACATTTAAAGGAGATTGTTTCAAAATATTTTTGCAAATTGAGATAAGGACAGAAAGATTG AGAAACATTGTATATTTTGCAAAAACAAGATGTTTGTAGCTGTTTCAGAGAGAGTACGGTATATTTATGGTAATTTTATCCACTAGCAAATCTTGATTTA GTTTGATAGTGTGTGGAATTTTATTTTGAAGGATAAGACCATGGGAAAATTGTGGTAAAGACTGTTTGTACCCTTCATGAAATAATTCTGAAGTTGCCAT CAGTTTTACTAATCTTCTGTGAAATGCATAGATATGCGCATGTTCAACTTTTTATTGTGGTCTTATAATTAAATGTAAAATTGAAAATTCATTTGCTGTT TCAAAGTGTGATATCTTTCACAATAGCCTTTTTATAGTCAGTAATTCAGAATAATCAAGTTCATATGGATAAATGCATTTTTATTTCCTATTTCTTTAGG GAGTGCTACAAATGTTTGTCACTTAAATTTCAAGTTTCTGTTTTAATAGTTAACTGACTATAGATTGTTTTCTATGCCATGTATGTGCCACTTCTGAGAG TAGTAAATGACTCTTTGCTACATTTTAAAAGCAATTGTATTAGTAAGAACTTTGTAAATAAATACCTAAAACCCAAGTGTA

ECT2.F11 (forward primer)
ECT2.R10 (reverse primer)
Alternative donor

Frame 0

RLTSRANRHGS*REQLSF*IGCGGRRRGMAVFV RGVGV*RGGTPRAFLRVTESTSCYLDWSAVARSR LTATSASRVQAILLPQPPE*LGLQVPATKPS*F LYF**RRGFIVLARLVSNS*PRDPPALASQSAR ITSVSHRVRPFKWYF*FSSSSP*SS*FRRIQIMA ENSVLTSTTGRTSLADSSIFDSKVTEISKENLL IGSTSYVEEMPQIETRVILVQEAGKQEELIKAL KDIKVGFVKMESVEEFEGLDSPEFENVFVVTDFQ DSVFNDLYKADCRVIGPPVVLNCSQKGEPLPFS CRPLYCTSMMNLVLCFTGFRKKEELVRLVTLVH HMGGVIRKDFNSKVTHLVANCTQGEKFRVAVSLG TPIMKPEWIYKAWERRNEQDFYAAVDDFRNEFK VPPFQDCILSFLGFSDEEKTNMEEMTEMQGGKY LPLGDERCTHLVVEENIVKDLPFEPSKKLYVVKQ EWFWGSIQMDARAGETMYLYEKANTPELKKSVS MLSLNTPNSNRKRRRLKETLAQLSRETDVSPFP PRKRPSAEHSLSIGSLLDISNTPESSINYGDTPK SCTKSSKSSTPVPSKQSARWQVAKELYQTESNY VNILATIIQLFQVPLEEEGQRGGPILAPEEIKT IFGSIPDIFDVHTKIKDDLEDLIVNWDESKSIGD IFLKYSKDLVKTYPPFVNFFEMSKETIIKCEKQ KPRFHAFLKINQAKPECGRQSLVELLIRPVQRL PSVALLLNDLKKHTADENPDKSTLEKAIGSLKEV MTHINEDKRKTEAQKQIFDVVYEVDGCPANLLS SHRSLVQRVETISLGEHPCDRGEQVTLFLFNDC LEIARKRHKVIGTFRSPHGQTRPPASLKHIHLMP LSQIKKVLDIRETEDCHNAFALLVRPPTEQANV LLSFQMTSDELPKENWLKMLCRHVANTICKADA ENLIYTADPESFEVNTKDMDSTLSRASRAIKKTS KKVTRAFSFSKTPKRALRRALMTSHGSVEGRSP SSNDKHVMSRLSSTSSLAGIPSPSLVSLPSFFE RRSHTLSRSTTHLI*SVTKILNYRNV*TPHTQIR N*LKWYL*LALGESWKEDK*H*TMLFDFSS*KS KVYLLHFQVNSCKK***F*CNLSLV*ICHSKAN NLSCYQLILVALQP**SK*ILWAGAKYCCESICI VSMNEFMEIDICAAQFMQRLNDIIILDENLHRI LIK*WVCFTCAV*SI*ITTPFTVYFLLKRFQDL ACGF*KICPH*QE*HLKEIVSKYFCKLR*GQKD* ETLYILQKQDVCSCFRESTVYLW*FYPLANLDL V**CVEFYFEG*DHGKIVVKTVCTLHEIILKLP SVLLIFCEMHRYAHVQLFIVVL*LNVKLKIHLLF QSVISFTIAFL*SVIQNNQVHMDKCIFISYFFR ECYKCLSLKFQVSVLIVN*L*IVFYAMYVPLLR VVNDSLLHFKSNCISKNFVNKYLKPKC

Frame 1

G*HHAPIGMALRESSLVFESVVAAAGEEWRYL* EESAFEEVELLGLF*E*RSLPLVT*TGVQWHDL GSLQPLPPGFKRFSCLSLLSSWDYRCLPPSPANF CIFSRDGVSLCWPGWSRTPDLVIRPPWPPKVLG LQV*ATASGLSNGIFDFPLPVLKAADLEEYKSW LKIVY*HPLLGGLAWQTLPFLILKLLRFPRKTYL LDLLHM*KRCLRLKQE*YWFKKLENKKNL*KP* RTLKWAL*RWSQWKNLKVWILRNLKMYL*SRTF RILSLMTSTRLIVELLDHQLY*IVHKKESLCHFH VARCIVQV**I*YYALLDLGKKKN*SGW*HWSI TWVELFEKTLIQKLHIWWQIVHKEKNSGLL*V* VLQL*SQNGFIKLGKGGMNRISMQQLMTLEMNLK FLHFKIVF*VSWDFQMKRKPIWKK*LKCKEVNI YRLEMKDALTL*LKRI**KIFPLNLQRNFMLSS KSGSGEAFKWMPELEKLCIYMKRQILLSSRNQCQ CFL*IPLTAIANDVV*KKHLLSFQERQTCHHFH PVSAHQLSIPFP*GHS*ISPTHQSLALTMETPQ SLVLSLLKAPLQFLQSSQQGGKLQKSFIKLKVIM LIYWQQLFSYFKYHWKRKDNVVDLSLHQRRLRL FLVASQISLMYTLR*RMILKTL*LIGMRAKALV TFF*NIQKIW*KPTLPL*TSLK*ARKQLLNVKNR NQDFMLFSR*TKQNQNVDGRALLNFLSDQYRGY PVLHYF*MILRSIQLMKIQTKAL*KKLLDH*RK **RILMRIREKQKLKSKFLMLFMK*MDAQLIFYL LTEA*YSGLKQFL*VSTPVTEENK*LSSSSMIA *R*QENGTRLLALLGVLMAKPDPQLLLSIFT*C LFLRLRRYWT*ERQKIAIMLLPCL*GHQQSRQMC YSVSR*HQMNFQKKTG*RCCVDM*LTPFVKQML RILFILLIQNPLK*IQKIWTVH*VEHQEQ*KRL QKRLQEHSLSPKLQKELFEGLL*HPTAQWREEVL PAMISM**VVFLAHHH*QVSLLPPLSAFLPSLK GEVIR*VDLQLI*YEALPKS*IIEMYRHLILK* ETDLNGTCN*HLVKAGRKINNTKLCYLIFLLERV RFTCYIFKLIHVKNDSDFDVIYLLFESVIQRPI I*VAIS*Y**LCNPDRVNKFYGRVPNTAVNLFV *YP*MNLWK*IFVQLNLCRD*MTS*YWMKTCIEF *LNSGSVSHVQFEVFK*PLLSQFIFFSSVFKI* HVDFKRFALINKNNI*RRLFQNIFAN*DKDRKI EKHCIFCKNKMFVAVSERVRYIYGNFIH*QILI* FDSVWNFILKDKTMGKLW*RLFVPFMK*F*SCH QFY*SSVKCIDMRMFNFLLWSYN*M*N*KFICC FKV*YLSQ*PFYSQ*FRIIKFIWINAFLFPISLG SATNVCHLNFKFLF**LTDYRLFSMPCMCHF*E **MTLCYILKAIVLVRTL*INT*NPSV

Frame 2

VDITRQSAWLLERAA*FLNRLWRPPARNGGICE RSRRLKRWNS*GFFESDGVYLLLPRLECSGTIS AHCNLCLPGSSDSPASAS*VAGITGACHQAQLI FVFLVETGFHCVGQAGLELLTS*SARLGLPKC*D YKCEPPRPAFQMVFLIFLFQSLKQLI*KNTNHG *K*CINIHYWED*LGRLFHF*F*SY*DFQGKLT YWIYFICRRDASD*NKSDIGSRSWKTRRTYKSLK GH*SGLCKDGVSGRI*RFGFSGI*KCICSHGLS GFCL**PLQG*L*SYWTTSCIKLFTKRRAFAIF MSPVVLYKYDESSTMLYWI*EKRRTSQVGDIGPS HGWSYSKRL*FKSYTFGGKLYTRRKIQGCCESR YSNYEARMDL*SLGKAE*TGFLCSS**L*K*I* SSSISRLYFKFPGIFR*RENQYGRND*NARR*IF TAWR*KMHSPCS*REYSKRSSL*TFKETLCCQA RVVLGKHSNGCPSWRNYVFI*KGKYS*AQEISV NAFSKYP*QQSQTTSFKRNTCSAFKRDRRVTIST P*APIS*AFPFHRVTPRYLQHTRV*H*LWRHPK VLY*VF*KLHSSSFKAVSKVASCKRALSN*K*L C*YIGNNYSVISSTIGRGRTTWWTYPCTRGD*DY FW*HPRYL*CTH*DKG*S*RPYS*LG*EQKHW* HFSEIFKRFGKNLPSLCKLL*NEQGNNY*M*KT ETKISCFSQDKPSKTRMWTAEPC*TSYPTSTEVT QCCITFK*S*EAYS**KSRQKHFRKSYWITEGS NDAY**G*EKNRSSKANF*CCL*SRWMPS*SFI FSPKLSTAG*NNFSR*APL*QRRTSNSLPLQ*LP RDSKKTAQGYWHF*ESSWPNPTPSFS*AYSPNA SFSD*EGIGHKRDRRLP*CFCLACEATNRAGKC ATQFPDDIR*TSKRKLAKDAVSTCS*HHL*SRC* ESYLYC*SRIL*SKYKRYGQYIE*SIKSNKKDF KKGYKSILFLQNSKKSSSKGSYDIPRLSGGKKS FQQ**ACNESSF*HIIISRYPFSLPCQPSFLL*K EKSYVK*IYNSFDMKRYQNLKL*KCIDTSYSNK KLT*MVLVISTW*KLEGR*ITLNYAI*FFFLKE *GLPVTFSS*FM*KMIVILM*FISCLNLSFKGQ* FKLLSADISSFATLIE*INFMGGCQILL*IYLY SIHE*IYGNRYLCSSIYAEIK*HHNTG*KLA*N SD*IVGLFHMCSLKYLNNHSFHSLFSSQAFSRSS MWILKDLPSLTRITFKGDCFKIFLQIEIRTERL RNIVYFAKTRCL*LFQREYGIFMVILSTSKS*F SLIVCGILF*RIRPWENCGKDCLYPS*NNSEVAI SFTNLL*NA*ICACSTFYCGLIIKCKIENSFAV SKCDIFHNSLFIVSNSE*SSSYG*MHFYFLFL* GVLQMFVT*ISSFCFNS*LTIDCFLCHVCATSES SK*LFATF*KQLY**ELCK*IPKTQV