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Sequences

Sequence LGALS9.fAug05

TGTTTGTTAAATTATATGTAGGGACAGGGTCTTCCAACCTTTCCCGAGCTGGTCTCAAGCTCCTGGGCTGAAGTGATCCTCCTGCCTCAGCCTCTCAAAG TGCTGGTATTATAGGCATAAGCCACCACGCCCTGCCCATTTTTCTAAGTTGGATTATTTTACACTCCAGTCTGCTGTGAATGAGAGTTCCAGTTGTTGCG TATCGCAATCAATTTGTGATTGCCTACAAATGATGTGCCGTGATGAAATTGTCCCTGGTGTCCGAGAGGATCGCAAAGAATTTCCCTTCCTTTCTCTCCT TTCTGCTCCCCTTGTACTGTCTACTCCTTAAACTGGTACTTGATCCAGGAACATAATGGGGATGGACAGTGCAGATGGCACACATGGCCTCAGTCTATTG GAGCATCTCAAAGGAATTAGTCTGCAAATTCTAGTGCATCTAGGTGGAGCACAGGAAGGAAGAGAGGTGCAGACTCACGTATGGCCCTGGGGCACCTGCC ATCCTCTCGCACCCGTACAGCCCTTACCTGCCAGCACTTACCCTACCCAGCTTGAGTTCTGGGCCTTCTCACCAGCACATCGCTGGGACCTGGTCGACTT CAGGTAAGTTTATTTCATGGCTCCCTCCCCCACGACCTGCACATGCCATATGTTCCTTCCCAGCCCCATGTCACTTGAAATGTTCTATCATGTCCAAGAC TTCTCTCCTCATCCTAAAGTGCAGAAAATAAGGATGGAGAAGAGAAGGAGAAGAGAATCAAGAGAGATTCATTGAGAGGGAGGATCAGAAGCTCCTGGGC ACCCCATACTGTAGAATAAAAATATTCATTATGGTGTTTTTCTTGCAAAATATTCCCCTCTACATAATTATGTTTTTACATGAATAATATAAATTTAAAC AGGAGAAATACTTTTTGAAGAGTCTGAGAATCTTCATAAAGTTCAGGCGGCCACGAATTAGCTGTGTGACCTGGGGCATGCCCCTTAGACTCTGAGCCTC AGCTTCTCTGTATGTAAAATGGGTTGAAGCCGCCCTTCCCCACAAGCACCCTGTGCACAGGCAATGCCCAGCCCCATTATTTTCTGGACTCCGTGGCCAA GCATGCTTAGGACACACAGCCACATACTTCTGGGCAGTGTCATCTGGCAACTTGCTGTCATGTCAGTGTGGTCAAGCATTGTAGACCTCTATGAACCAAA TATGCTTCAGGCTTGGGTTGAGCACAGGAGAGGGAGGAGGGAAAGGTCACTGGGGCTGGGAGTGCCTACTTCCCTCTGTGAGTGTCACCCCAGTCCACCC AGTACCATACCTTTCTCTCTCTGGACCCACTTCCTTTTGCTGCCGGCTCCTCCCCATTGAATAACAGCCAAGTTGCTTTGGTTTCTATTTCTTTGTTAAG TCGTTCCCTCTACAAAGGACTTCCTAGTGGGTGTGAAAGGCAGCGGTGGCCACAGAGGCGGCGGAGAGATGGCCTTCAGCGGTTCCCAGGCTCCCTACCT GAGTCCAGCTGTCCCCTTTTCTGGGACTATTCAAGGAGGTCTCCAGGACGGACTTCAGATCACTGTCAATGGGACCGTTCTCAGCTCCAGTGGAACCAGG TTTGCTGTGAACTTTCAGACTGGCTTCAGTGGAAATGACATTGCCTTCCACTTCAACCCTCGGTTTGAAGATGGAGGGTACGTGGTGTGCAACACGAGGC AGAACGGAAGCTGGGGGCCCGAGGAGAGGAAGACACACATGCCTTTCCAGAAGGGGATGCCCTTTGACCTCTGCTTCCTGGTGCAGAGCTCAGATTTCAA GGTGATGGTGAACGGGATCCTCTTCGTGCAGTACTTCCACCGCGTGCCCTTCCACCGTGTGGACACCATCTCCGTCAATGGCTCTGTGCAGCTGTCCTAC ATCAGCTTCCAGACCCAGACAGTCATCCACACAGTGCAGAGCGCCCCTGGACAGATGTTCTCTACTCCCGCCATCCCACCTATGATGTACCCCCACCCCG CCTATCCGATGCCTTTCATCACCACCATTCTGGGAGGGCTGTACCCATCCAAGTCCATCCTCCTGTCAGGCACTGTCCTGCCCAGTGCTCAGAGGTTCCA CATCAACCTGTGCTCTGGGAACCACATCGCCTTCCACCTGAACCCCCGTTTTGATGAGAATGCTGTGGTCCGCAACACCCAGATCGACAACTCCTGGGGG TCTGAGGAGCGAAGTCTGCCCCGAAAAATGCCCTTCGTCCGTGGCCAGAGCTTCTCAGTAAGGCACCGCAGTCTGGAGCTTGGAGAGGCTCCCATGGGTG CACAGGGGGAGGGGGTAAAGGAGGTTTGAGGTACCTTGAACAGTATGGGGGCTGATTCCTCTGGGATGAAGGCCCAAAAGAAGAGAAGGTGGCCAACAAA TTATTATTATTATTACTGTCCCGCATGAAGGAAGTGCTGGGATCTGCTTTTTCAGATGAGGAAACAAACTCACAAGTGCAGGTTACTTGCTTGTGGTCAC ACAGTCAGTAAGTGTCAAATCAGACCCAAGAAGGAGGTGTTATTCATTTCTTCATTTACTCAACAACCATTTTCTTGTCGCCTGCTTTGCGCAGGAAGCA CCCCACATGCTGGGGGCACAGTCATGAAGCACACAGACAGGGTCCTGCCTCCAGGGAGTTTATAATTTAGGTGGGGAAAGAACAAGGAAAGTAAAGCATG GACAGATTGTCACAGATAACGACAGTGCCACGCACAAAGCAAAACAGAGCGATGAGATAGAGCGAGCCTGGGAGGGGCAGCTAGGGCCAACGTGCAGAGG CACGGGTGTGGCCTGTCCAGGAACAGCAGGCAGGCGTGCTCCACCCTGACTACATATTCAAATCACCCAGAGAGCTTACAAAGGAAATATCAGTGCTGGG ACCCCAGCCCAGGCCAATGAGGCAGTCAGCTGGGCATTCGTATTTTAAAAATATGCCTTAAGTGATTATACTCTGCAGTCAGAGTTGAAAATGACTGATG TAGGGCTTTGCAGCCATGGAAAGGAGCTTGGATTTTAACACAGTGAAAAGCCTCAGGAGTGTTTCAAGCCAGGGAGCAACATACTTTGATTTGTAGACAG CTGTGTGACCTCGGACAAGTGACTTAATTATTCTGTGCTTCAGTTTCCTCATCTACAAAATGGAGCTAATAATCATGAATTTCCTAGAGTTGTAAGAATG GAATGAGACAGTATCTGTAGTATGCTTAGGACAGTGCCTGGCAGGTGGCATGTGTGATAGGAGTGTTAGCTATTGCAAAGGGAAGGAGGTTTTAACATTT GTACCCTGACTGCTTCGTGGAGAAGGCATTGGGGTGGAAAAGACAAGTTAGGAGACTGATGCTGCAGGAGCCCAGGCCCAGAGCACTCCTGTGTGCTGGG CCTGAGGGATACAGACGGCACAGGCCAGTGCTCCTGTCCCTGATGGGGTGGGAACAGTATTCTAGACCAGGGAACAGTACAGGGGAAAGAGCAC

LGALS9.F5 (forward primer)
LGALS9.R1 (reverse primer)
Single exon skipping

Frame 0

CLLNYM*GQGLPTFPELVSSSWAEVILLPQPLK VLVL*A*ATTPCPFF*VGLFYTPVCCE*EFQLLR IAINL*LPTNDVP**NCPWCPRGSQRISLPFSP FCSPCTVYSLNWYLIQEHNGDGQCRWHTWPQSI GASQRN*SANSSASRWSTGRKRGADSRMALGHLP SSRTRTALTCQHLPYPA*VLGLLTSTSLGPGRL QVSLFHGSLPHDLHMPYVPSQPHVT*NVLSCPR LLSSS*SAENKDGEEKEKRIKRDSLRGRIRSSWA PHTVE*KYSLWCFSCKIFPST*LCFYMNNINLN RRNTF*RV*ESS*SSGGHELAV*PGACPLDSEP QLLCM*NGLKPPFPTSTLCTGNAQPHYFLDSVAK HA*DTQPHTSGQCHLATCCHVSVVKHCRPL*TK YASGLG*AQEREEGKVTGAGSAYFPL*VSPQST QYHTFLSLDPLPFAAGSSPLNNSQVALVSISLLS RSLYKGLPSGCERQRWPQRRRRDGLQRFPGSLP ESSCPLFWDYSRRSPGRTSDHCQWDRSQLQWNQ VCCELSDWLQWK*HCLPLQPSV*RWRVRGVQHEA ERKLGARGEEDTHAFPEGDAL*PLLPGAELRFQ GDGERDPLRAVLPPRALPPCGHHLRQWLCAAVL HQLPDPDSHPHSAERPWTDVLYSRHPTYDVPPPR LSDAFHHHHSGRAVPIQVHPPVRHCPAQCSEVP HQPVLWEPHRLPPEPPF**ECCGPQHPDRQLLG V*GAKSAPKNALRPWPELLSKAPQSGAWRGSHGC TGGGGKGGLRYLEQYGG*FLWDEGPKEEKVANK LLLLLLSRMKEVLGSAFSDEETNSQVQVTCLWS HSQ*VSNQTQEGGVIHFFIYSTTIFLSPALRRKH PTCWGHSHEAHRQGPASREFII*VGKEQGK*SM DRLSQITTVPRTKQNRAMR*SEPGRGS*GQRAE ARVWPVQEQQAGVLHPDYIFKSPRELTKEISVLG PQPRPMRQSAGHSYFKNMP*VIILCSQS*K*LM *GFAAMERSLDFNTVKSLRSVSSQGATYFDL*T AV*PRTSDLIILCFSFLIYKMELIIMNFLEL*EW NETVSVVCLGQCLAGGMCDRSVSYCKGKEVLTF VP*LLRGEGIGVEKTS*ETDAAGAQAQSTPVCW A*GIQTAQASAPVPDGVGTVF*TREQYRGKS

Frame 1

VC*IICRDRVFQPFPSWSQAPGLK*SSCLSLSK CWYYRHKPPRPAHFSKLDYFTLQSAVNESSSCC VSQSICDCLQMMCRDEIVPGVREDRKEFPFLSLL SAPLVLSTP*TGT*SRNIMGMDSADGTHGLSLL EHLKGISLQILVHLGGAQEGREVQTHVWPWGTC HPLAPVQPLPASTYPTQLEFWAFSPAHRWDLVDF R*VYFMAPSPTTCTCHMFLPSPMSLEMFYHVQD FSPHPKVQKIRMEKRRRRESREIH*EGGSEAPG HPIL*NKNIHYGVFLAKYSPLHNYVFT*II*I*T GEILFEESENLHKVQAATN*LCDLGHAP*TLSL SFSVCKMG*SRPSPQAPCAQAMPSPIIFWTPWP SMLRTHSHILLGSVIWQLAVMSVWSSIVDLYEPN MLQAWVEHRRGRRERSLGLGVPTSLCECHPSPP STIPFSLWTHFLLLPAPPH*ITAKLLWFLFLC* VVPSTKDFLVGVKGSGGHRGGGEMAFSGSQAPYL SPAVPFSGTIQGGLQDGLQITVNGTVLSSSGTR FAVNFQTGFSGNDIAFHFNPRFEDGGYVVCNTR QNGSWGPEERKTHMPFQKGMPFDLCFLVQSSDFK VMVNGILFVQYFHRVPFHRVDTISVNGSVQLSY ISFQTQTVIHTVQSAPGQMFSTPAIPPMMYPHP AYPMPFITTILGGLYPSKSILLSGTVLPSAQRFH INLCSGNHIAFHLNPRFDENAVVRNTQIDNSWG SEERSLPRKMPFVRGQSFSVRHRSLELGEAPMG AQGEGVKEV*GTLNSMGADSSGMKAQKKRRWPTN YYYYYCPA*RKCWDLLFQMRKQTHKCRLLACGH TVSKCQIRPKKEVLFISSFTQQPFSCRLLCAGS TPHAGGTVMKHTDRVLPPGSL*FRWGKNKESKAW TDCHR*RQCHAQSKTER*DRASLGGAARANVQR HGCGLSRNSRQACSTLTTYSNHPESLQRKYQCW DPSPGQ*GSQLGIRILKICLK*LYSAVRVEND*C RALQPWKGAWILTQ*KASGVFQAREQHTLICRQ LCDLGQVT*LFCASVSSSTKWS**S*IS*SCKN GMRQYL*YA*DSAWQVACVIGVLAIAKGRRF*HL YPDCFVEKALGWKRQVRRLMLQEPRPRALLCAG PEGYRRHRPVLLSLMGWEQYSRPGNSTGERA

Frame 2

FVKLYVGTGSSNLSRAGLKLLG*SDPPASASQS AGIIGISHHALPIFLSWIILHSSLL*MRVPVVA YRNQFVIAYK*CAVMKLSLVSERIAKNFPSFLS FLLPLYCLLLKLVLDPGT*WGWTVQMAHMASVYW SISKELVCKF*CI*VEHRKEERCRLTYGPGAPA ILSHPYSPYLPALTLPSLSSGPSHQHIAGTWST SGKFISWLPPPRPAHAICSFPAPCHLKCSIMSKT SLLILKCRK*GWRREGEENQERFIEREDQKLLG TPYCRIKIFIMVFFLQNIPLYIIMFLHE*YKFK QEKYFLKSLRIFIKFRRPRISCVTWGMPLRL*AS ASLYVKWVEAALPHKHPVHRQCPAPLFSGLRGQ ACLGHTATYFWAVSSGNLLSCQCGQAL*TSMNQ ICFRLGLSTGEGGGKGHWGWECLLPSVSVTPVHP VPYLSLSGPTSFCCRLLPIE*QPSCFGFYFFVK SFPLQRTS*WV*KAAVATEAAERWPSAVPRLPT *VQLSPFLGLFKEVSRTDFRSLSMGPFSAPVEPG LL*TFRLASVEMTLPSTSTLGLKMEGTWCATRG RTEAGGPRRGRHTCLSRRGCPLTSASWCRAQIS R*W*TGSSSCSTSTACPSTVWTPSPSMALCSCPT SASRPRQSSTQCRAPLDRCSLLPPSHL*CTPTP PIRCLSSPPFWEGCTHPSPSSCQALSCPVLRGS TSTCALGTTSPST*TPVLMRMLWSATPRSTTPGG LRSEVCPEKCPSSVARASQ*GTAVWSLERLPWV HRGRG*RRFEVP*TVWGLIPLG*RPKRREGGQQ IIIIITVPHEGSAGICFFR*GNKLTSAGYLLVVT QSVSVKSDPRRRCYSFLHLLNNHFLVACFAQEA PHMLGAQS*STQTGSCLQGVYNLGGERTRKVKH GQIVTDNDSATHKAKQSDEIERAWEGQLGPTCRG TGVACPGTAGRRAPP*LHIQITQRAYKGNISAG TPAQANEAVSWAFVF*KYALSDYTLQSELKMTD VGLCSHGKELGF*HSEKPQECFKPGSNIL*FVDS CVTSDK*LNYSVLQFPHLQNGANNHEFPRVVRM E*DSICSMLRTVPGRWHV**EC*LLQREGGFNI CTLTASWRRHWGGKDKLGD*CCRSPGPEHSCVLG LRDTDGTGQCSCP*WGGNSILDQGTVQGKEH

Sequence LGALS9.eAug05

TGTTTGTTAAATTATATGTAGGGACAGGGTCTTCCAACCTTTCCCGAGCTGGTCTCAAGCTCCTGGGCTGAAGTGATCCTCCTGCCTCAGCCTCTCAAAG TGCTGGTATTATAGGCATAAGCCACCACGCCCTGCCCATTTTTCTAAGTTGGATTATTTTACACTCCAGTCTGCTGTGAATGAGAGTTCCAGTTGTTGCG TATCGCAATCAATTTGTGATTGCCTACAAATGATGTGCCGTGATGAAATTGTCCCTGGTGTCCGAGAGGATCGCAAAGAATTTCCCTTCCTTTCTCTCCT TTCTGCTCCCCTTGTACTGTCTACTCCTTAAACTGGTACTTGATCCAGGAACATAATGGGGATGGACAGTGCAGATGGCACACATGGCCTCAGTCTATTG GAGCATCTCAAAGGAATTAGTCTGCAAATTCTAGTGCATCTAGGTGGAGCACAGGAAGGAAGAGAGGTGCAGACTCACGTATGGCCCTGGGGCACCTGCC ATCCTCTCGCACCCGTACAGCCCTTACCTGCCAGCACTTACCCTACCCAGCTTGAGTTCTGGGCCTTCTCACCAGCACATCGCTGGGACCTGGTCGACTT CAGGTAAGTTTATTTCATGGCTCCCTCCCCCACGACCTGCACATGCCATATGTTCCTTCCCAGCCCCATGTCACTTGAAATGTTCTATCATGTCCAAGAC TTCTCTCCTCATCCTAAAGTGCAGAAAATAAGGATGGAGAAGAGAAGGAGAAGAGAATCAAGAGAGATTCATTGAGAGGGAGGATCAGAAGCTCCTGGGC ACCCCATACTGTAGAATAAAAATATTCATTATGGTGTTTTTCTTGCAAAATATTCCCCTCTACATAATTATGTTTTTACATGAATAATATAAATTTAAAC AGGAGAAATACTTTTTGAAGAGTCTGAGAATCTTCATAAAGTTCAGGCGGCCACGAATTAGCTGTGTGACCTGGGGCATGCCCCTTAGACTCTGAGCCTC AGCTTCTCTGTATGTAAAATGGGTTGAAGCCGCCCTTCCCCACAAGCACCCTGTGCACAGGCAATGCCCAGCCCCATTATTTTCTGGACTCCGTGGCCAA GCATGCTTAGGACACACAGCCACATACTTCTGGGCAGTGTCATCTGGCAACTTGCTGTCATGTCAGTGTGGTCAAGCATTGTAGACCTCTATGAACCAAA TATGCTTCAGGCTTGGGTTGAGCACAGGAGAGGGAGGAGGGAAAGGTCACTGGGGCTGGGAGTGCCTACTTCCCTCTGTGAGTGTCACCCCAGTCCACCC AGTACCATACCTTTCTCTCTCTGGACCCACTTCCTTTTGCTGCCGGCTCCTCCCCATTGAATAACAGCCAAGTTGCTTTGGTTTCTATTTCTTTGTTAAG TCGTTCCCTCTACAAAGGACTTCCTAGTGGGTGTGAAAGGCAGCGGTGGCCACAGAGGCGGCGGAGAGATGGCCTTCAGCGGTTCCCAGGCTCCCTACCT GAGTCCAGCTGTCCCCTTTTCTGGGACTATTCAAGGAGGTCTCCAGGACGGACTTCAGATCACTGTCAATGGGACCGTTCTCAGCTCCAGTGGAACCAGG TTTGCTGTGAACTTTCAGACTGGCTTCAGTGGAAATGACATTGCCTTCCACTTCAACCCTCGGTTTGAAGATGGAGGGTACGTGGTGTGCAACACGAGGC AGAACGGAAGCTGGGGGCCCGAGGAGAGGAAGACACACATGCCTTTCCAGAAGGGGATGCCCTTTGACCTCTGCTTCCTGGTGCAGAGCTCAGATTTCAA GGTGATGGTGAACGGGATCCTCTTCGTGCAGTACTTCCACCGCGTGCCCTTCCACCGTGTGGACACCATCTCCGTCAATGGCTCTGTGCAGCTGTCCTAC ATCAGCTTCCAGACCCAGACAGTCATCCACACAGTGCAGAGCGCCCCTGGACAGATGTTCTCTACTCCCGCCATCCCACCTATGATGTACCCCCACCCCG CCTATCCGATGCCTTTCATCACCACCATTCTGGGAGGGCTGTACCCATCCAAGTCCATCCTCCTGTCAGGCACTGTCCTGCCCAGTGCTCAGAGGTGTGG ATCTTGTGTGAAGCTCACTGCCTCAAGGTGGCCGTGGATGGTCAGCACCTGTTTGAATACTACCATCGCCTGAGGAACCTGCCCACCATCAACAGACTGG AAGTGGGGGGCGACATCCAGCTGACCCATGTGCAGACATAGGCGGCTTCCTGGCCCTGGGGCCGGGGGCTGGGGTGTGGGGCAGTCTGGGTCCTCTCATC ATCCCCACTTCCCAGGCCCAGCCTTTCCAACCCTGCCTGGGATCTGGGCTTTAATGCAGAGGCCATGTCCTTGTCTGGTCCTGCTTCTGGCTACAGCCAC CCTGGAACGGAGAAGGCAGCTGACGGGGATTGCCTTCCTCAGCCGCAGCAGCACCTGGGGCTCCAGCTGCTGGAATCCTACCATCCCAGGAGGCAGGCAC AGCCAGGGAGAGGGGAGGAGTGGGCAGTGAAGATGAAGCCCCATGCTCAGTCCCCTCCCATCCCCCACGCAGCTCCACCCCAGTCCCAAGCCACCAGCTG TCTGCTCCTGGTGGGAGGTGGCCTCCTCAGCCCCTCCTCTCTGACCTTTAACCTCACTCTCACCTTGCACCGTGCACCAACCCTTCACCCCTCCTGGAAA GCAGGCCTGATGGCTTCCCACTGGCCTCCACCACCTGACCAGAGTGTTCTCTTCAGAGGACTGGCTCCTTTCCCAGTGTCCTTAAAATAAAGAAATGAAA ATGCTTGTTGGCACATTCA

LGALS9.F5 (forward primer)
LGALS9.R1 (reverse primer)
Single exon skipping

Frame 0

CLLNYM*GQGLPTFPELVSSSWAEVILLPQPLK VLVL*A*ATTPCPFF*VGLFYTPVCCE*EFQLLR IAINL*LPTNDVP**NCPWCPRGSQRISLPFSP FCSPCTVYSLNWYLIQEHNGDGQCRWHTWPQSI GASQRN*SANSSASRWSTGRKRGADSRMALGHLP SSRTRTALTCQHLPYPA*VLGLLTSTSLGPGRL QVSLFHGSLPHDLHMPYVPSQPHVT*NVLSCPR LLSSS*SAENKDGEEKEKRIKRDSLRGRIRSSWA PHTVE*KYSLWCFSCKIFPST*LCFYMNNINLN RRNTF*RV*ESS*SSGGHELAV*PGACPLDSEP QLLCM*NGLKPPFPTSTLCTGNAQPHYFLDSVAK HA*DTQPHTSGQCHLATCCHVSVVKHCRPL*TK YASGLG*AQEREEGKVTGAGSAYFPL*VSPQST QYHTFLSLDPLPFAAGSSPLNNSQVALVSISLLS RSLYKGLPSGCERQRWPQRRRRDGLQRFPGSLP ESSCPLFWDYSRRSPGRTSDHCQWDRSQLQWNQ VCCELSDWLQWK*HCLPLQPSV*RWRVRGVQHEA ERKLGARGEEDTHAFPEGDAL*PLLPGAELRFQ GDGERDPLRAVLPPRALPPCGHHLRQWLCAAVL HQLPDPDSHPHSAERPWTDVLYSRHPTYDVPPPR LSDAFHHHHSGRAVPIQVHPPVRHCPAQCSEVW ILCEAHCLKVAVDGQHLFEYYHRLRNLPTINRL EVGGDIQLTHVQT*AASWPWGRGLGCGAVWVLSS SPLPRPSLSNPAWDLGFNAEAMSLSGPASGYSH PGTEKAADGDCLPQPQQHLGLQLLESYHPRRQA QPGRGEEWAVKMKPHAQSPPIPHAAPPQSQATSC LLLVGGGLLSPSSLTFNLTLTLHRAPTLHPSWK AGLMASHWPPPPDQSVLFRGLAPFPVSLK*RNE NACWHI

Frame 1

VC*IICRDRVFQPFPSWSQAPGLK*SSCLSLSK CWYYRHKPPRPAHFSKLDYFTLQSAVNESSSCC VSQSICDCLQMMCRDEIVPGVREDRKEFPFLSLL SAPLVLSTP*TGT*SRNIMGMDSADGTHGLSLL EHLKGISLQILVHLGGAQEGREVQTHVWPWGTC HPLAPVQPLPASTYPTQLEFWAFSPAHRWDLVDF R*VYFMAPSPTTCTCHMFLPSPMSLEMFYHVQD FSPHPKVQKIRMEKRRRRESREIH*EGGSEAPG HPIL*NKNIHYGVFLAKYSPLHNYVFT*II*I*T GEILFEESENLHKVQAATN*LCDLGHAP*TLSL SFSVCKMG*SRPSPQAPCAQAMPSPIIFWTPWP SMLRTHSHILLGSVIWQLAVMSVWSSIVDLYEPN MLQAWVEHRRGRRERSLGLGVPTSLCECHPSPP STIPFSLWTHFLLLPAPPH*ITAKLLWFLFLC* VVPSTKDFLVGVKGSGGHRGGGEMAFSGSQAPYL SPAVPFSGTIQGGLQDGLQITVNGTVLSSSGTR FAVNFQTGFSGNDIAFHFNPRFEDGGYVVCNTR QNGSWGPEERKTHMPFQKGMPFDLCFLVQSSDFK VMVNGILFVQYFHRVPFHRVDTISVNGSVQLSY ISFQTQTVIHTVQSAPGQMFSTPAIPPMMYPHP AYPMPFITTILGGLYPSKSILLSGTVLPSAQRCG SCVKLTASRWPWMVSTCLNTTIA*GTCPPSTDW KWGATSS*PMCRHRRLPGPGAGGWGVGQSGSSH HPHFPGPAFPTLPGIWALMQRPCPCLVLLLATAT LERRRQLTGIAFLSRSSTWGSSCWNPTIPGGRH SQGEGRSGQ*R*SPMLSPLPSPTQLHPSPKPPA VCSWWEVASSAPPL*PLTSLSPCTVHQPFTPPGK QA*WLPTGLHHLTRVFSSEDWLLSQCP*NKEMK MLVGTF

Frame 2

FVKLYVGTGSSNLSRAGLKLLG*SDPPASASQS AGIIGISHHALPIFLSWIILHSSLL*MRVPVVA YRNQFVIAYK*CAVMKLSLVSERIAKNFPSFLS FLLPLYCLLLKLVLDPGT*WGWTVQMAHMASVYW SISKELVCKF*CI*VEHRKEERCRLTYGPGAPA ILSHPYSPYLPALTLPSLSSGPSHQHIAGTWST SGKFISWLPPPRPAHAICSFPAPCHLKCSIMSKT SLLILKCRK*GWRREGEENQERFIEREDQKLLG TPYCRIKIFIMVFFLQNIPLYIIMFLHE*YKFK QEKYFLKSLRIFIKFRRPRISCVTWGMPLRL*AS ASLYVKWVEAALPHKHPVHRQCPAPLFSGLRGQ ACLGHTATYFWAVSSGNLLSCQCGQAL*TSMNQ ICFRLGLSTGEGGGKGHWGWECLLPSVSVTPVHP VPYLSLSGPTSFCCRLLPIE*QPSCFGFYFFVK SFPLQRTS*WV*KAAVATEAAERWPSAVPRLPT *VQLSPFLGLFKEVSRTDFRSLSMGPFSAPVEPG LL*TFRLASVEMTLPSTSTLGLKMEGTWCATRG RTEAGGPRRGRHTCLSRRGCPLTSASWCRAQIS R*W*TGSSSCSTSTACPSTVWTPSPSMALCSCPT SASRPRQSSTQCRAPLDRCSLLPPSHL*CTPTP PIRCLSSPPFWEGCTHPSPSSCQALSCPVLRGV DLV*SSLPQGGRGWSAPV*ILPSPEEPAHHQQTG SGGRHPADPCADIGGFLALGPGAGVWGSLGPLI IPTSQAQPFQPCLGSGL*CRGHVLVWSCFWLQP PWNGEGS*RGLPSSAAAAPGAPAAGILPSQEAGT ARERGGVGSEDEAPCSVPSHPPRSSTPVPSHQL SAPGGRWPPQPLLSDL*PHSHLAPCTNPSPLLE SRPDGFPLASTT*PECSLQRTGSFPSVLKIKK*K CLLAHS

Sequence LGALS9.dAug05

TGTTTGTTAAATTATATGTAGGGACAGGGTCTTCCAACCTTTCCCGAGCTGGTCTCAAGCTCCTGGGCTGAAGTGATCCTCCTGCCTCAGCCTCTCAAAG TGCTGGTATTATAGGCATAAGCCACCACGCCCTGCCCATTTTTCTAAGTTGGATTATTTTACACTCCAGTCTGCTGTGAATGAGAGTTCCAGTTGTTGCG TATCGCAATCAATTTGTGATTGCCTACAAATGATGTGCCGTGATGAAATTGTCCCTGGTGTCCGAGAGGATCGCAAAGAATTTCCCTTCCTTTCTCTCCT TTCTGCTCCCCTTGTACTGTCTACTCCTTAAACTGGTACTTGATCCAGGAACATAATGGGGATGGACAGTGCAGATGGCACACATGGCCTCAGTCTATTG GAGCATCTCAAAGGAATTAGTCTGCAAATTCTAGTGCATCTAGGTGGAGCACAGGAAGGAAGAGAGGTGCAGACTCACGTATGGCCCTGGGGCACCTGCC ATCCTCTCGCACCCGTACAGCCCTTACCTGCCAGCACTTACCCTACCCAGCTTGAGTTCTGGGCCTTCTCACCAGCACATCGCTGGGACCTGGTCGACTT CAGGTAAGTTTATTTCATGGCTCCCTCCCCCACGACCTGCACATGCCATATGTTCCTTCCCAGCCCCATGTCACTTGAAATGTTCTATCATGTCCAAGAC TTCTCTCCTCATCCTAAAGTGCAGAAAATAAGGATGGAGAAGAGAAGGAGAAGAGAATCAAGAGAGATTCATTGAGAGGGAGGATCAGAAGCTCCTGGGC ACCCCATACTGTAGAATAAAAATATTCATTATGGTGTTTTTCTTGCAAAATATTCCCCTCTACATAATTATGTTTTTACATGAATAATATAAATTTAAAC AGGAGAAATACTTTTTGAAGAGTCTGAGAATCTTCATAAAGTTCAGGCGGCCACGAATTAGCTGTGTGACCTGGGGCATGCCCCTTAGACTCTGAGCCTC AGCTTCTCTGTATGTAAAATGGGTTGAAGCCGCCCTTCCCCACAAGCACCCTGTGCACAGGCAATGCCCAGCCCCATTATTTTCTGGACTCCGTGGCCAA GCATGCTTAGGACACACAGCCACATACTTCTGGGCAGTGTCATCTGGCAACTTGCTGTCATGTCAGTGTGGTCAAGCATTGTAGACCTCTATGAACCAAA TATGCTTCAGGCTTGGGTTGAGCACAGGAGAGGGAGGAGGGAAAGGTCACTGGGGCTGGGAGTGCCTACTTCCCTCTGTGAGTGTCACCCCAGTCCACCC AGTACCATACCTTTCTCTCTCTGGACCCACTTCCTTTTGCTGCCGGCTCCTCCCCATTGAATAACAGCCAAGTTGCTTTGGTTTCTATTTCTTTGTTAAG TCGTTCCCTCTACAAAGGACTTCCTAGTGGGTGTGAAAGGCAGCGGTGGCCACAGAGGCGGCGGAGAGATGGCCTTCAGCGGTTCCCAGGCTCCCTACCT GAGTCCAGCTGTCCCCTTTTCTGGGACTATTCAAGGAGGTCTCCAGGACGGACTTCAGATCACTGTCAATGGGACCGTTCTCAGCTCCAGTGGAACCAGG TTTGCTGTGAACTTTCAGACTGGCTTCAGTGGAAATGACATTGCCTTCCACTTCAACCCTCGGTTTGAAGATGGAGGGTACGTGGTGTGCAACACGAGGC AGAACGGAAGCTGGGGGCCCGAGGAGAGGAAGACACACATGCCTTTCCAGAAGGGGATGCCCTTTGACCTCTGCTTCCTGGTGCAGAGCTCAGATTTCAA GGTGATGGTGAACGGGATCCTCTTCGTGCAGTACTTCCACCGCGTGCCCTTCCACCGTGTGGACACCATCTCCGTCAATGGCTCTGTGCAGCTGTCCTAC ATCAGCTTCCAGCCTCCCGGCGTGTGGCCTGCCAACCCGGCTCCCATTACCCAGACAGTCATCCACACAGTGCAGAGCGCCCCTGGACAGATGTTCTCTA CTCCCGCCATCCCACCTATGATGTACCCCCACCCCGCCTATCCGATGCCTTTCATCACCACCATTCTGGGAGGGCTGTACCCATCCAAGTCCATCCTCCT GTCAGGCACTGTCCTGCCCAGTGCTCAGAGGTGTGGATCTTGTGTGAAGCTCACTGCCTCAAGGTGGCCGTGGATGGTCAGCACCTGTTTGAATACTACC ATCGCCTGAGGAACCTGCCCACCATCAACAGACTGGAAGTGGGGGGCGACATCCAGCTGACCCATGTGCAGACATAGGCGGCTTCCTGGCCCTGGGGCCG GGGGCTGGGGTGTGGGGCAGTCTGGGTCCTCTCATCATCCCCACTTCCCAGGCCCAGCCTTTCCAACCCTGCCTGGGATCTGGGCTTTAATGCAGAGGCC ATGTCCTTGTCTGGTCCTGCTTCTGGCTACAGCCACCCTGGAACGGAGAAGGCAGCTGACGGGGATTGCCTTCCTCAGCCGCAGCAGCACCTGGGGCTCC AGCTGCTGGAATCCTACCATCCCAGGAGGCAGGCACAGCCAGGGAGAGGGGAGGAGTGGGCAGTGAAGATGAAGCCCCATGCTCAGTCCCCTCCCATCCC CCACGCAGCTCCACCCCAGTCCCAAGCCACCAGCTGTCTGCTCCTGGTGGGAGGTGGCCTCCTCAGCCCCTCCTCTCTGACCTTTAACCTCACTCTCACC TTGCACCGTGCACCAACCCTTCACCCCTCCTGGAAAGCAGGCCTGATGGCTTCCCACTGGCCTCCACCACCTGACCAGAGTGTTCTCTTCAGAGGACTGG CTCCTTTCCCAGTGTCCTTAAAATAAAGAAATGAAAATGCTTGTTGGCACATTCA

LGALS9.F5 (forward primer)
LGALS9.R1 (reverse primer)
Single exon skipping

Frame 0

CLLNYM*GQGLPTFPELVSSSWAEVILLPQPLK VLVL*A*ATTPCPFF*VGLFYTPVCCE*EFQLLR IAINL*LPTNDVP**NCPWCPRGSQRISLPFSP FCSPCTVYSLNWYLIQEHNGDGQCRWHTWPQSI GASQRN*SANSSASRWSTGRKRGADSRMALGHLP SSRTRTALTCQHLPYPA*VLGLLTSTSLGPGRL QVSLFHGSLPHDLHMPYVPSQPHVT*NVLSCPR LLSSS*SAENKDGEEKEKRIKRDSLRGRIRSSWA PHTVE*KYSLWCFSCKIFPST*LCFYMNNINLN RRNTF*RV*ESS*SSGGHELAV*PGACPLDSEP QLLCM*NGLKPPFPTSTLCTGNAQPHYFLDSVAK HA*DTQPHTSGQCHLATCCHVSVVKHCRPL*TK YASGLG*AQEREEGKVTGAGSAYFPL*VSPQST QYHTFLSLDPLPFAAGSSPLNNSQVALVSISLLS RSLYKGLPSGCERQRWPQRRRRDGLQRFPGSLP ESSCPLFWDYSRRSPGRTSDHCQWDRSQLQWNQ VCCELSDWLQWK*HCLPLQPSV*RWRVRGVQHEA ERKLGARGEEDTHAFPEGDAL*PLLPGAELRFQ GDGERDPLRAVLPPRALPPCGHHLRQWLCAAVL HQLPASRRVACQPGSHYPDSHPHSAERPWTDVLY SRHPTYDVPPPRLSDAFHHHHSGRAVPIQVHPP VRHCPAQCSEVWILCEAHCLKVAVDGQHLFEYY HRLRNLPTINRLEVGGDIQLTHVQT*AASWPWGR GLGCGAVWVLSSSPLPRPSLSNPAWDLGFNAEA MSLSGPASGYSHPGTEKAADGDCLPQPQQHLGL QLLESYHPRRQAQPGRGEEWAVKMKPHAQSPPIP HAAPPQSQATSCLLLVGGGLLSPSSLTFNLTLT LHRAPTLHPSWKAGLMASHWPPPPDQSVLFRGL APFPVSLK*RNENACWHI

Frame 1

VC*IICRDRVFQPFPSWSQAPGLK*SSCLSLSK CWYYRHKPPRPAHFSKLDYFTLQSAVNESSSCC VSQSICDCLQMMCRDEIVPGVREDRKEFPFLSLL SAPLVLSTP*TGT*SRNIMGMDSADGTHGLSLL EHLKGISLQILVHLGGAQEGREVQTHVWPWGTC HPLAPVQPLPASTYPTQLEFWAFSPAHRWDLVDF R*VYFMAPSPTTCTCHMFLPSPMSLEMFYHVQD FSPHPKVQKIRMEKRRRRESREIH*EGGSEAPG HPIL*NKNIHYGVFLAKYSPLHNYVFT*II*I*T GEILFEESENLHKVQAATN*LCDLGHAP*TLSL SFSVCKMG*SRPSPQAPCAQAMPSPIIFWTPWP SMLRTHSHILLGSVIWQLAVMSVWSSIVDLYEPN MLQAWVEHRRGRRERSLGLGVPTSLCECHPSPP STIPFSLWTHFLLLPAPPH*ITAKLLWFLFLC* VVPSTKDFLVGVKGSGGHRGGGEMAFSGSQAPYL SPAVPFSGTIQGGLQDGLQITVNGTVLSSSGTR FAVNFQTGFSGNDIAFHFNPRFEDGGYVVCNTR QNGSWGPEERKTHMPFQKGMPFDLCFLVQSSDFK VMVNGILFVQYFHRVPFHRVDTISVNGSVQLSY ISFQPPGVWPANPAPITQTVIHTVQSAPGQMFS TPAIPPMMYPHPAYPMPFITTILGGLYPSKSILL SGTVLPSAQRCGSCVKLTASRWPWMVSTCLNTT IA*GTCPPSTDWKWGATSS*PMCRHRRLPGPGA GGWGVGQSGSSHHPHFPGPAFPTLPGIWALMQRP CPCLVLLLATATLERRRQLTGIAFLSRSSTWGS SCWNPTIPGGRHSQGEGRSGQ*R*SPMLSPLPS PTQLHPSPKPPAVCSWWEVASSAPPL*PLTSLSP CTVHQPFTPPGKQA*WLPTGLHHLTRVFSSEDW LLSQCP*NKEMKMLVGTF

Frame 2

FVKLYVGTGSSNLSRAGLKLLG*SDPPASASQS AGIIGISHHALPIFLSWIILHSSLL*MRVPVVA YRNQFVIAYK*CAVMKLSLVSERIAKNFPSFLS FLLPLYCLLLKLVLDPGT*WGWTVQMAHMASVYW SISKELVCKF*CI*VEHRKEERCRLTYGPGAPA ILSHPYSPYLPALTLPSLSSGPSHQHIAGTWST SGKFISWLPPPRPAHAICSFPAPCHLKCSIMSKT SLLILKCRK*GWRREGEENQERFIEREDQKLLG TPYCRIKIFIMVFFLQNIPLYIIMFLHE*YKFK QEKYFLKSLRIFIKFRRPRISCVTWGMPLRL*AS ASLYVKWVEAALPHKHPVHRQCPAPLFSGLRGQ ACLGHTATYFWAVSSGNLLSCQCGQAL*TSMNQ ICFRLGLSTGEGGGKGHWGWECLLPSVSVTPVHP VPYLSLSGPTSFCCRLLPIE*QPSCFGFYFFVK SFPLQRTS*WV*KAAVATEAAERWPSAVPRLPT *VQLSPFLGLFKEVSRTDFRSLSMGPFSAPVEPG LL*TFRLASVEMTLPSTSTLGLKMEGTWCATRG RTEAGGPRRGRHTCLSRRGCPLTSASWCRAQIS R*W*TGSSSCSTSTACPSTVWTPSPSMALCSCPT SASSLPACGLPTRLPLPRQSSTQCRAPLDRCSL LPPSHL*CTPTPPIRCLSSPPFWEGCTHPSPSS CQALSCPVLRGVDLV*SSLPQGGRGWSAPV*ILP SPEEPAHHQQTGSGGRHPADPCADIGGFLALGP GAGVWGSLGPLIIPTSQAQPFQPCLGSGL*CRG HVLVWSCFWLQPPWNGEGS*RGLPSSAAAAPGAP AAGILPSQEAGTARERGGVGSEDEAPCSVPSHP PRSSTPVPSHQLSAPGGRWPPQPLLSDL*PHSH LAPCTNPSPLLESRPDGFPLASTT*PECSLQRTG SFPSVLKIKK*KCLLAHS

Sequence LGALS9.cAug05

TGTTTGTTAAATTATATGTAGGGACAGGGTCTTCCAACCTTTCCCGAGCTGGTCTCAAGCTCCTGGGCTGAAGTGATCCTCCTGCCTCAGCCTCTCAAAG TGCTGGTATTATAGGCATAAGCCACCACGCCCTGCCCATTTTTCTAAGTTGGATTATTTTACACTCCAGTCTGCTGTGAATGAGAGTTCCAGTTGTTGCG TATCGCAATCAATTTGTGATTGCCTACAAATGATGTGCCGTGATGAAATTGTCCCTGGTGTCCGAGAGGATCGCAAAGAATTTCCCTTCCTTTCTCTCCT TTCTGCTCCCCTTGTACTGTCTACTCCTTAAACTGGTACTTGATCCAGGAACATAATGGGGATGGACAGTGCAGATGGCACACATGGCCTCAGTCTATTG GAGCATCTCAAAGGAATTAGTCTGCAAATTCTAGTGCATCTAGGTGGAGCACAGGAAGGAAGAGAGGTGCAGACTCACGTATGGCCCTGGGGCACCTGCC ATCCTCTCGCACCCGTACAGCCCTTACCTGCCAGCACTTACCCTACCCAGCTTGAGTTCTGGGCCTTCTCACCAGCACATCGCTGGGACCTGGTCGACTT CAGGTAAGTTTATTTCATGGCTCCCTCCCCCACGACCTGCACATGCCATATGTTCCTTCCCAGCCCCATGTCACTTGAAATGTTCTATCATGTCCAAGAC TTCTCTCCTCATCCTAAAGTGCAGAAAATAAGGATGGAGAAGAGAAGGAGAAGAGAATCAAGAGAGATTCATTGAGAGGGAGGATCAGAAGCTCCTGGGC ACCCCATACTGTAGAATAAAAATATTCATTATGGTGTTTTTCTTGCAAAATATTCCCCTCTACATAATTATGTTTTTACATGAATAATATAAATTTAAAC AGGAGAAATACTTTTTGAAGAGTCTGAGAATCTTCATAAAGTTCAGGCGGCCACGAATTAGCTGTGTGACCTGGGGCATGCCCCTTAGACTCTGAGCCTC AGCTTCTCTGTATGTAAAATGGGTTGAAGCCGCCCTTCCCCACAAGCACCCTGTGCACAGGCAATGCCCAGCCCCATTATTTTCTGGACTCCGTGGCCAA GCATGCTTAGGACACACAGCCACATACTTCTGGGCAGTGTCATCTGGCAACTTGCTGTCATGTCAGTGTGGTCAAGCATTGTAGACCTCTATGAACCAAA TATGCTTCAGGCTTGGGTTGAGCACAGGAGAGGGAGGAGGGAAAGGTCACTGGGGCTGGGAGTGCCTACTTCCCTCTGTGAGTGTCACCCCAGTCCACCC AGTACCATACCTTTCTCTCTCTGGACCCACTTCCTTTTGCTGCCGGCTCCTCCCCATTGAATAACAGCCAAGTTGCTTTGGTTTCTATTTCTTTGTTAAG TCGTTCCCTCTACAAAGGACTTCCTAGTGGGTGTGAAAGGCAGCGGTGGCCACAGAGGCGGCGGAGAGATGGCCTTCAGCGGTTCCCAGGCTCCCTACCT GAGTCCAGCTGTCCCCTTTTCTGGGACTATTCAAGGAGGTCTCCAGGACGGACTTCAGATCACTGTCAATGGGACCGTTCTCAGCTCCAGTGGAACCAGA TGGAGGGTACGTGGTGTGCAACACGAGGCAGAACGGAAGCTGGGGGCCCGAGGAGAGGAAGACACACATGCCTTTCCAGAAGGGGATGCCCTTTGACCTC TGCTTCCTGGTGCAGAGCTCAGATTTCAAGGTGATGGTGAACGGGATCCTCTTCGTGCAGTACTTCCACCGCGTGCCCTTCCACCGTGTGGACACCATCT CCGTCAATGGCTCTGTGCAGCTGTCCTACATCAGCTTCCAGCCTCCCGGCGTGTGGCCTGCCAACCCGGCTCCCATTACCCAGACAGTCATCCACACAGT GCAGAGCGCCCCTGGACAGATGTTCTCTACTCCCGCCATCCCACCTATGATGTACCCCCACCCCGCCTATCCGATGCCTTTCATCACCACCATTCTGGGA GGGCTGTACCCATCCAAGTCCATCCTCCTGTCAGGCACTGTCCTGCCCAGTGCTCAGAGGTTCCACATCAACCTGTGCTCTGGGAACCACATCGCCTTCC ACCTGAACCCCCGTTTTGATGAGAATGCTGTGGTCCGCAACACCCAGATCGACAACTCCTGGGGGTCTGAGGAGCGAAGTCTGCCCCGAAAAATGCCCTT CGTCCGTGGCCAGAGCTTCTCAGTGTGGATCTTGTGTGAAGCTCACTGCCTCAAGGTGGCCGTGGATGGTCAGCACCTGTTTGAATACTACCATCGCCTG AGGAACCTGCCCACCATCAACAGACTGGAAGTGGGGGGCGACATCCAGCTGACCCATGTGCAGACATAGGCGGCTTCCTGGCCCTGGGGCCGGGGGCTGG GGTGTGGGGCAGTCTGGGTCCTCTCATCATCCCCACTTCCCAGGCCCAGCCTTTCCAACCCTGCCTGGGATCTGGGCTTTAATGCAGAGGCCATGTCCTT GTCTGGTCCTGCTTCTGGCTACAGCCACCCTGGAACGGAGAAGGCAGCTGACGGGGATTGCCTTCCTCAGCCGCAGCAGCACCTGGGGCTCCAGCTGCTG GAATCCTACCATCCCAGGAGGCAGGCACAGCCAGGGAGAGGGGAGGAGTGGGCAGTGAAGATGAAGCCCCATGCTCAGTCCCCTCCCATCCCCCACGCAG CTCCACCCCAGTCCCAAGCCACCAGCTGTCTGCTCCTGGTGGGAGGTGGCCTCCTCAGCCCCTCCTCTCTGACCTTTAACCTCACTCTCACCTTGCACCG TGCACCAACCCTTCACCCCTCCTGGAAAGCAGGCCTGATGGCTTCCCACTGGCCTCCACCACCTGACCAGAGTGTTCTCTTCAGAGGACTGGCTCCTTTC CCAGTGTCCTTAAAATAAAGAAATGAAAATGCTTGTTGGCACATTCA

LGALS9.F5 (forward primer)
LGALS9.R1 (reverse primer)
Single exon skipping

Frame 0

CLLNYM*GQGLPTFPELVSSSWAEVILLPQPLK VLVL*A*ATTPCPFF*VGLFYTPVCCE*EFQLLR IAINL*LPTNDVP**NCPWCPRGSQRISLPFSP FCSPCTVYSLNWYLIQEHNGDGQCRWHTWPQSI GASQRN*SANSSASRWSTGRKRGADSRMALGHLP SSRTRTALTCQHLPYPA*VLGLLTSTSLGPGRL QVSLFHGSLPHDLHMPYVPSQPHVT*NVLSCPR LLSSS*SAENKDGEEKEKRIKRDSLRGRIRSSWA PHTVE*KYSLWCFSCKIFPST*LCFYMNNINLN RRNTF*RV*ESS*SSGGHELAV*PGACPLDSEP QLLCM*NGLKPPFPTSTLCTGNAQPHYFLDSVAK HA*DTQPHTSGQCHLATCCHVSVVKHCRPL*TK YASGLG*AQEREEGKVTGAGSAYFPL*VSPQST QYHTFLSLDPLPFAAGSSPLNNSQVALVSISLLS RSLYKGLPSGCERQRWPQRRRRDGLQRFPGSLP ESSCPLFWDYSRRSPGRTSDHCQWDRSQLQWNQ MEGTWCATRGRTEAGGPRRGRHTCLSRRGCPLTS ASWCRAQISR*W*TGSSSCSTSTACPSTVWTPS PSMALCSCPTSASSLPACGLPTRLPLPRQSSTQ CRAPLDRCSLLPPSHL*CTPTPPIRCLSSPPFWE GCTHPSPSSCQALSCPVLRGSTSTCALGTTSPS T*TPVLMRMLWSATPRSTTPGGLRSEVCPEKCP SSVARASQCGSCVKLTASRWPWMVSTCLNTTIA* GTCPPSTDWKWGATSS*PMCRHRRLPGPGAGGW GVGQSGSSHHPHFPGPAFPTLPGIWALMQRPCP CLVLLLATATLERRRQLTGIAFLSRSSTWGSSCW NPTIPGGRHSQGEGRSGQ*R*SPMLSPLPSPTQ LHPSPKPPAVCSWWEVASSAPPL*PLTSLSPCT VHQPFTPPGKQA*WLPTGLHHLTRVFSSEDWLLS QCP*NKEMKMLVGTF

Frame 1

VC*IICRDRVFQPFPSWSQAPGLK*SSCLSLSK CWYYRHKPPRPAHFSKLDYFTLQSAVNESSSCC VSQSICDCLQMMCRDEIVPGVREDRKEFPFLSLL SAPLVLSTP*TGT*SRNIMGMDSADGTHGLSLL EHLKGISLQILVHLGGAQEGREVQTHVWPWGTC HPLAPVQPLPASTYPTQLEFWAFSPAHRWDLVDF R*VYFMAPSPTTCTCHMFLPSPMSLEMFYHVQD FSPHPKVQKIRMEKRRRRESREIH*EGGSEAPG HPIL*NKNIHYGVFLAKYSPLHNYVFT*II*I*T GEILFEESENLHKVQAATN*LCDLGHAP*TLSL SFSVCKMG*SRPSPQAPCAQAMPSPIIFWTPWP SMLRTHSHILLGSVIWQLAVMSVWSSIVDLYEPN MLQAWVEHRRGRRERSLGLGVPTSLCECHPSPP STIPFSLWTHFLLLPAPPH*ITAKLLWFLFLC* VVPSTKDFLVGVKGSGGHRGGGEMAFSGSQAPYL SPAVPFSGTIQGGLQDGLQITVNGTVLSSSGTR WRVRGVQHEAERKLGARGEEDTHAFPEGDAL*P LLPGAELRFQGDGERDPLRAVLPPRALPPCGHHL RQWLCAAVLHQLPASRRVACQPGSHYPDSHPHS AERPWTDVLYSRHPTYDVPPPRLSDAFHHHHSG RAVPIQVHPPVRHCPAQCSEVPHQPVLWEPHRLP PEPPF**ECCGPQHPDRQLLGV*GAKSAPKNAL RPWPELLSVDLV*SSLPQGGRGWSAPV*ILPSP EEPAHHQQTGSGGRHPADPCADIGGFLALGPGAG VWGSLGPLIIPTSQAQPFQPCLGSGL*CRGHVL VWSCFWLQPPWNGEGS*RGLPSSAAAAPGAPAA GILPSQEAGTARERGGVGSEDEAPCSVPSHPPRS STPVPSHQLSAPGGRWPPQPLLSDL*PHSHLAP CTNPSPLLESRPDGFPLASTT*PECSLQRTGSF PSVLKIKK*KCLLAHS

Frame 2

FVKLYVGTGSSNLSRAGLKLLG*SDPPASASQS AGIIGISHHALPIFLSWIILHSSLL*MRVPVVA YRNQFVIAYK*CAVMKLSLVSERIAKNFPSFLS FLLPLYCLLLKLVLDPGT*WGWTVQMAHMASVYW SISKELVCKF*CI*VEHRKEERCRLTYGPGAPA ILSHPYSPYLPALTLPSLSSGPSHQHIAGTWST SGKFISWLPPPRPAHAICSFPAPCHLKCSIMSKT SLLILKCRK*GWRREGEENQERFIEREDQKLLG TPYCRIKIFIMVFFLQNIPLYIIMFLHE*YKFK QEKYFLKSLRIFIKFRRPRISCVTWGMPLRL*AS ASLYVKWVEAALPHKHPVHRQCPAPLFSGLRGQ ACLGHTATYFWAVSSGNLLSCQCGQAL*TSMNQ ICFRLGLSTGEGGGKGHWGWECLLPSVSVTPVHP VPYLSLSGPTSFCCRLLPIE*QPSCFGFYFFVK SFPLQRTS*WV*KAAVATEAAERWPSAVPRLPT *VQLSPFLGLFKEVSRTDFRSLSMGPFSAPVEPD GGYVVCNTRQNGSWGPEERKTHMPFQKGMPFDL CFLVQSSDFKVMVNGILFVQYFHRVPFHRVDTI SVNGSVQLSYISFQPPGVWPANPAPITQTVIHTV QSAPGQMFSTPAIPPMMYPHPAYPMPFITTILG GLYPSKSILLSGTVLPSAQRFHINLCSGNHIAF HLNPRFDENAVVRNTQIDNSWGSEERSLPRKMPF VRGQSFSVWILCEAHCLKVAVDGQHLFEYYHRL RNLPTINRLEVGGDIQLTHVQT*AASWPWGRGL GCGAVWVLSSSPLPRPSLSNPAWDLGFNAEAMSL SGPASGYSHPGTEKAADGDCLPQPQQHLGLQLL ESYHPRRQAQPGRGEEWAVKMKPHAQSPPIPHA APPQSQATSCLLLVGGGLLSPSSLTFNLTLTLHR APTLHPSWKAGLMASHWPPPPDQSVLFRGLAPF PVSLK*RNENACWHI

Sequence LGALS9.bAug05

TGTTTGTTAAATTATATGTAGGGACAGGGTCTTCCAACCTTTCCCGAGCTGGTCTCAAGCTCCTGGGCTGAAGTGATCCTCCTGCCTCAGCCTCTCAAAG TGCTGGTATTATAGGCATAAGCCACCACGCCCTGCCCATTTTTCTAAGTTGGATTATTTTACACTCCAGTCTGCTGTGAATGAGAGTTCCAGTTGTTGCG TATCGCAATCAATTTGTGATTGCCTACAAATGATGTGCCGTGATGAAATTGTCCCTGGTGTCCGAGAGGATCGCAAAGAATTTCCCTTCCTTTCTCTCCT TTCTGCTCCCCTTGTACTGTCTACTCCTTAAACTGGTACTTGATCCAGGAACATAATGGGGATGGACAGTGCAGATGGCACACATGGCCTCAGTCTATTG GAGCATCTCAAAGGAATTAGTCTGCAAATTCTAGTGCATCTAGGTGGAGCACAGGAAGGAAGAGAGGTGCAGACTCACGTATGGCCCTGGGGCACCTGCC ATCCTCTCGCACCCGTACAGCCCTTACCTGCCAGCACTTACCCTACCCAGCTTGAGTTCTGGGCCTTCTCACCAGCACATCGCTGGGACCTGGTCGACTT CAGGTAAGTTTATTTCATGGCTCCCTCCCCCACGACCTGCACATGCCATATGTTCCTTCCCAGCCCCATGTCACTTGAAATGTTCTATCATGTCCAAGAC TTCTCTCCTCATCCTAAAGTGCAGAAAATAAGGATGGAGAAGAGAAGGAGAAGAGAATCAAGAGAGATTCATTGAGAGGGAGGATCAGAAGCTCCTGGGC ACCCCATACTGTAGAATAAAAATATTCATTATGGTGTTTTTCTTGCAAAATATTCCCCTCTACATAATTATGTTTTTACATGAATAATATAAATTTAAAC AGGAGAAATACTTTTTGAAGAGTCTGAGAATCTTCATAAAGTTCAGGCGGCCACGAATTAGCTGTGTGACCTGGGGCATGCCCCTTAGACTCTGAGCCTC AGCTTCTCTGTATGTAAAATGGGTTGAAGCCGCCCTTCCCCACAAGCACCCTGTGCACAGGCAATGCCCAGCCCCATTATTTTCTGGACTCCGTGGCCAA GCATGCTTAGGACACACAGCCACATACTTCTGGGCAGTGTCATCTGGCAACTTGCTGTCATGTCAGTGTGGTCAAGCATTGTAGACCTCTATGAACCAAA TATGCTTCAGGCTTGGGTTGAGCACAGGAGAGGGAGGAGGGAAAGGTCACTGGGGCTGGGAGTGCCTACTTCCCTCTGTGAGTGTCACCCCAGTCCACCC AGTACCATACCTTTCTCTCTCTGGACCCACTTCCTTTTGCTGCCGGCTCCTCCCCATTGAATAACAGCCAAGTTGCTTTGGTTTCTATTTCTTTGTTAAG TCGTTCCCTCTACAAAGGACTTCCTAGTGGGTGTGAAAGGCAGCGGTGGCCACAGAGGCGGCGGAGAGATGGCCTTCAGCGGTTCCCAGGCTCCCTACCT GAGTCCAGCTGTCCCCTTTTCTGGGACTATTCAAGGAGGTCTCCAGGACGGACTTCAGATCACTGTCAATGGGACCGTTCTCAGCTCCAGTGGAACCAGG TTTGCTGTGAACTTTCAGACTGGCTTCAGTGGAAATGACATTGCCTTCCACTTCAACCCTCGGTTTGAAGATGGAGGGTACGTGGTGTGCAACACGAGGC AGAACGGAAGCTGGGGGCCCGAGGAGAGGAAGACACACATGCCTTTCCAGAAGGGGATGCCCTTTGACCTCTGCTTCCTGGTGCAGAGCTCAGATTTCAA GGTGATGGTGAACGGGATCCTCTTCGTGCAGTACTTCCACCGCGTGCCCTTCCACCGTGTGGACACCATCTCCGTCAATGGCTCTGTGCAGCTGTCCTAC ATCAGCTTCCAGCCTCCCGGCGTGTGGCCTGCCAACCCGGCTCCCATTACCCAGACAGTCATCCACACAGTGCAGAGCGCCCCTGGACAGATGTTCTCTA CTCCCGCCATCCCACCTATGATGTACCCCCACCCCGCCTATCCGATGCCTTTCATCACCACCATTCTGGGAGGGCTGTACCCATCCAAGTCCATCCTCCT GTCAGGCACTGTCCTGCCCAGTGCTCAGAGGTTCCACATCAACCTGTGCTCTGGGAACCACATCGCCTTCCACCTGAACCCCCGTTTTGATGAGAATGCT GTGGTCCGCAACACCCAGATCGACAACTCCTGGGGGTCTGAGGAGCGAAGTCTGCCCCGAAAAATGCCCTTCGTCCGTGGCCAGAGCTTCTCAGTGTGGA TCTTGTGTGAAGCTCACTGCCTCAAGGTGGCCGTGGATGGTCAGCACCTGTTTGAATACTACCATCGCCTGAGGAACCTGCCCACCATCAACAGACTGGA AGTGGGGGGCGACATCCAGCTGACCCATGTGCAGACATAGGCGGCTTCCTGGCCCTGGGGCCGGGGGCTGGGGTGTGGGGCAGTCTGGGTCCTCTCATCA TCCCCACTTCCCAGGCCCAGCCTTTCCAACCCTGCCTGGGATCTGGGCTTTAATGCAGAGGCCATGTCCTTGTCTGGTCCTGCTTCTGGCTACAGCCACC CTGGAACGGAGAAGGCAGCTGACGGGGATTGCCTTCCTCAGCCGCAGCAGCACCTGGGGCTCCAGCTGCTGGAATCCTACCATCCCAGGAGGCAGGCACA GCCAGGGAGAGGGGAGGAGTGGGCAGTGAAGATGAAGCCCCATGCTCAGTCCCCTCCCATCCCCCACGCAGCTCCACCCCAGTCCCAAGCCACCAGCTGT CTGCTCCTGGTGGGAGGTGGCCTCCTCAGCCCCTCCTCTCTGACCTTTAACCTCACTCTCACCTTGCACCGTGCACCAACCCTTCACCCCTCCTGGAAAG CAGGCCTGATGGCTTCCCACTGGCCTCCACCACCTGACCAGAGTGTTCTCTTCAGAGGACTGGCTCCTTTCCCAGTGTCCTTAAAATAAAGAAATGAAAA TGCTTGTTGGCACATTCA

LGALS9.F5 (forward primer)
LGALS9.R1 (reverse primer)
Single exon skipping

Frame 0

CLLNYM*GQGLPTFPELVSSSWAEVILLPQPLK VLVL*A*ATTPCPFF*VGLFYTPVCCE*EFQLLR IAINL*LPTNDVP**NCPWCPRGSQRISLPFSP FCSPCTVYSLNWYLIQEHNGDGQCRWHTWPQSI GASQRN*SANSSASRWSTGRKRGADSRMALGHLP SSRTRTALTCQHLPYPA*VLGLLTSTSLGPGRL QVSLFHGSLPHDLHMPYVPSQPHVT*NVLSCPR LLSSS*SAENKDGEEKEKRIKRDSLRGRIRSSWA PHTVE*KYSLWCFSCKIFPST*LCFYMNNINLN RRNTF*RV*ESS*SSGGHELAV*PGACPLDSEP QLLCM*NGLKPPFPTSTLCTGNAQPHYFLDSVAK HA*DTQPHTSGQCHLATCCHVSVVKHCRPL*TK YASGLG*AQEREEGKVTGAGSAYFPL*VSPQST QYHTFLSLDPLPFAAGSSPLNNSQVALVSISLLS RSLYKGLPSGCERQRWPQRRRRDGLQRFPGSLP ESSCPLFWDYSRRSPGRTSDHCQWDRSQLQWNQ VCCELSDWLQWK*HCLPLQPSV*RWRVRGVQHEA ERKLGARGEEDTHAFPEGDAL*PLLPGAELRFQ GDGERDPLRAVLPPRALPPCGHHLRQWLCAAVL HQLPASRRVACQPGSHYPDSHPHSAERPWTDVLY SRHPTYDVPPPRLSDAFHHHHSGRAVPIQVHPP VRHCPAQCSEVPHQPVLWEPHRLPPEPPF**EC CGPQHPDRQLLGV*GAKSAPKNALRPWPELLSVD LV*SSLPQGGRGWSAPV*ILPSPEEPAHHQQTG SGGRHPADPCADIGGFLALGPGAGVWGSLGPLI IPTSQAQPFQPCLGSGL*CRGHVLVWSCFWLQPP WNGEGS*RGLPSSAAAAPGAPAAGILPSQEAGT ARERGGVGSEDEAPCSVPSHPPRSSTPVPSHQL SAPGGRWPPQPLLSDL*PHSHLAPCTNPSPLLES RPDGFPLASTT*PECSLQRTGSFPSVLKIKK*K CLLAHS

Frame 1

VC*IICRDRVFQPFPSWSQAPGLK*SSCLSLSK CWYYRHKPPRPAHFSKLDYFTLQSAVNESSSCC VSQSICDCLQMMCRDEIVPGVREDRKEFPFLSLL SAPLVLSTP*TGT*SRNIMGMDSADGTHGLSLL EHLKGISLQILVHLGGAQEGREVQTHVWPWGTC HPLAPVQPLPASTYPTQLEFWAFSPAHRWDLVDF R*VYFMAPSPTTCTCHMFLPSPMSLEMFYHVQD FSPHPKVQKIRMEKRRRRESREIH*EGGSEAPG HPIL*NKNIHYGVFLAKYSPLHNYVFT*II*I*T GEILFEESENLHKVQAATN*LCDLGHAP*TLSL SFSVCKMG*SRPSPQAPCAQAMPSPIIFWTPWP SMLRTHSHILLGSVIWQLAVMSVWSSIVDLYEPN MLQAWVEHRRGRRERSLGLGVPTSLCECHPSPP STIPFSLWTHFLLLPAPPH*ITAKLLWFLFLC* VVPSTKDFLVGVKGSGGHRGGGEMAFSGSQAPYL SPAVPFSGTIQGGLQDGLQITVNGTVLSSSGTR FAVNFQTGFSGNDIAFHFNPRFEDGGYVVCNTR QNGSWGPEERKTHMPFQKGMPFDLCFLVQSSDFK VMVNGILFVQYFHRVPFHRVDTISVNGSVQLSY ISFQPPGVWPANPAPITQTVIHTVQSAPGQMFS TPAIPPMMYPHPAYPMPFITTILGGLYPSKSILL SGTVLPSAQRFHINLCSGNHIAFHLNPRFDENA VVRNTQIDNSWGSEERSLPRKMPFVRGQSFSVW ILCEAHCLKVAVDGQHLFEYYHRLRNLPTINRLE VGGDIQLTHVQT*AASWPWGRGLGCGAVWVLSS SPLPRPSLSNPAWDLGFNAEAMSLSGPASGYSH PGTEKAADGDCLPQPQQHLGLQLLESYHPRRQAQ PGRGEEWAVKMKPHAQSPPIPHAAPPQSQATSC LLLVGGGLLSPSSLTFNLTLTLHRAPTLHPSWK AGLMASHWPPPPDQSVLFRGLAPFPVSLK*RNEN ACWHI

Frame 2

FVKLYVGTGSSNLSRAGLKLLG*SDPPASASQS AGIIGISHHALPIFLSWIILHSSLL*MRVPVVA YRNQFVIAYK*CAVMKLSLVSERIAKNFPSFLS FLLPLYCLLLKLVLDPGT*WGWTVQMAHMASVYW SISKELVCKF*CI*VEHRKEERCRLTYGPGAPA ILSHPYSPYLPALTLPSLSSGPSHQHIAGTWST SGKFISWLPPPRPAHAICSFPAPCHLKCSIMSKT SLLILKCRK*GWRREGEENQERFIEREDQKLLG TPYCRIKIFIMVFFLQNIPLYIIMFLHE*YKFK QEKYFLKSLRIFIKFRRPRISCVTWGMPLRL*AS ASLYVKWVEAALPHKHPVHRQCPAPLFSGLRGQ ACLGHTATYFWAVSSGNLLSCQCGQAL*TSMNQ ICFRLGLSTGEGGGKGHWGWECLLPSVSVTPVHP VPYLSLSGPTSFCCRLLPIE*QPSCFGFYFFVK SFPLQRTS*WV*KAAVATEAAERWPSAVPRLPT *VQLSPFLGLFKEVSRTDFRSLSMGPFSAPVEPG LL*TFRLASVEMTLPSTSTLGLKMEGTWCATRG RTEAGGPRRGRHTCLSRRGCPLTSASWCRAQIS R*W*TGSSSCSTSTACPSTVWTPSPSMALCSCPT SASSLPACGLPTRLPLPRQSSTQCRAPLDRCSL LPPSHL*CTPTPPIRCLSSPPFWEGCTHPSPSS CQALSCPVLRGSTSTCALGTTSPST*TPVLMRML WSATPRSTTPGGLRSEVCPEKCPSSVARASQCG SCVKLTASRWPWMVSTCLNTTIA*GTCPPSTDW KWGATSS*PMCRHRRLPGPGAGGWGVGQSGSSHH PHFPGPAFPTLPGIWALMQRPCPCLVLLLATAT LERRRQLTGIAFLSRSSTWGSSCWNPTIPGGRH SQGEGRSGQ*R*SPMLSPLPSPTQLHPSPKPPAV CSWWEVASSAPPL*PLTSLSPCTVHQPFTPPGK QA*WLPTGLHHLTRVFSSEDWLLSQCP*NKEMK MLVGTF