Sequences - Rnomics 📊 Palace

        ☰
    
        Rnomics 📊 Palace
    
                       Back to Rnomics Platform
            
                       Open Session

1 warning:

Access restricted to public data.
Please sign in at Rnomics Platform to access your data.

Navigation

Home

Sequences

Sequence LGALS9.fAug05

TGTTTGTTAAATTATATGTAGGGACAGGGTCTTCCAACCTTTCCCGAGCTGGTCTCAAGCTCCTGGGCTGAAGTGATCCTCCTGCCTCAGCCTCTCAAAG TGCTGGTATTATAGGCATAAGCCACCACGCCCTGCCCATTTTTCTAAGTTGGATTATTTTACACTCCAGTCTGCTGTGAATGAGAGTTCCAGTTGTTGCG TATCGCAATCAATTTGTGATTGCCTACAAATGATGTGCCGTGATGAAATTGTCCCTGGTGTCCGAGAGGATCGCAAAGAATTTCCCTTCCTTTCTCTCCT TTCTGCTCCCCTTGTACTGTCTACTCCTTAAACTGGTACTTGATCCAGGAACATAATGGGGATGGACAGTGCAGATGGCACACATGGCCTCAGTCTATTG GAGCATCTCAAAGGAATTAGTCTGCAAATTCTAGTGCATCTAGGTGGAGCACAGGAAGGAAGAGAGGTGCAGACTCACGTATGGCCCTGGGGCACCTGCC ATCCTCTCGCACCCGTACAGCCCTTACCTGCCAGCACTTACCCTACCCAGCTTGAGTTCTGGGCCTTCTCACCAGCACATCGCTGGGACCTGGTCGACTT CAGGTAAGTTTATTTCATGGCTCCCTCCCCCACGACCTGCACATGCCATATGTTCCTTCCCAGCCCCATGTCACTTGAAATGTTCTATCATGTCCAAGAC TTCTCTCCTCATCCTAAAGTGCAGAAAATAAGGATGGAGAAGAGAAGGAGAAGAGAATCAAGAGAGATTCATTGAGAGGGAGGATCAGAAGCTCCTGGGC ACCCCATACTGTAGAATAAAAATATTCATTATGGTGTTTTTCTTGCAAAATATTCCCCTCTACATAATTATGTTTTTACATGAATAATATAAATTTAAAC AGGAGAAATACTTTTTGAAGAGTCTGAGAATCTTCATAAAGTTCAGGCGGCCACGAATTAGCTGTGTGACCTGGGGCATGCCCCTTAGACTCTGAGCCTC AGCTTCTCTGTATGTAAAATGGGTTGAAGCCGCCCTTCCCCACAAGCACCCTGTGCACAGGCAATGCCCAGCCCCATTATTTTCTGGACTCCGTGGCCAA GCATGCTTAGGACACACAGCCACATACTTCTGGGCAGTGTCATCTGGCAACTTGCTGTCATGTCAGTGTGGTCAAGCATTGTAGACCTCTATGAACCAAA TATGCTTCAGGCTTGGGTTGAGCACAGGAGAGGGAGGAGGGAAAGGTCACTGGGGCTGGGAGTGCCTACTTCCCTCTGTGAGTGTCACCCCAGTCCACCC AGTACCATACCTTTCTCTCTCTGGACCCACTTCCTTTTGCTGCCGGCTCCTCCCCATTGAATAACAGCCAAGTTGCTTTGGTTTCTATTTCTTTGTTAAG TCGTTCCCTCTACAAAGGACTTCCTAGTGGGTGTGAAAGGCAGCGGTGGCCACAGAGGCGGCGGAGAGATGGCCTTCAGCGGTTCCCAGGCTCCCTACCT GAGTCCAGCTGTCCCCTTTTCTGGGACTATTCAAGGAGGTCTCCAGGACGGACTTCAGATCACTGTCAATGGGACCGTTCTCAGCTCCAGTGGAACCAGG TTTGCTGTGAACTTTCAGACTGGCTTCAGTGGAAATGACATTGCCTTCCACTTCAACCCTCGGTTTGAAGATGGAGGGTACGTGGTGTGCAACACGAGGC AGAACGGAAGCTGGGGGCCCGAGGAGAGGAAGACACACATGCCTTTCCAGAAGGGGATGCCCTTTGACCTCTGCTTCCTGGTGCAGAGCTCAGATTTCAA GGTGATGGTGAACGGGATCCTCTTCGTGCAGTACTTCCACCGCGTGCCCTTCCACCGTGTGGACACCATCTCCGTCAATGGCTCTGTGCAGCTGTCCTAC ATCAGCTTCCAGACCCAGACAGTCATCCACACAGTGCAGAGCGCCCCTGGACAGATGTTCTCTACTCCCGCCATCCCACCTATGATGTACCCCCACCCCG CCTATCCGATGCCTTTCATCACCACCATTCTGGGAGGGCTGTACCCATCCAAGTCCATCCTCCTGTCAGGCACTGTCCTGCCCAGTGCTCAGAGGTTCCA CATCAACCTGTGCTCTGGGAACCACATCGCCTTCCACCTGAACCCCCGTTTTGATGAGAATGCTGTGGTCCGCAACACCCAGATCGACAACTCCTGGGGG TCTGAGGAGCGAAGTCTGCCCCGAAAAATGCCCTTCGTCCGTGGCCAGAGCTTCTCAGTAAGGCACCGCAGTCTGGAGCTTGGAGAGGCTCCCATGGGTG CACAGGGGGAGGGGGTAAAGGAGGTTTGAGGTACCTTGAACAGTATGGGGGCTGATTCCTCTGGGATGAAGGCCCAAAAGAAGAGAAGGTGGCCAACAAA TTATTATTATTATTACTGTCCCGCATGAAGGAAGTGCTGGGATCTGCTTTTTCAGATGAGGAAACAAACTCACAAGTGCAGGTTACTTGCTTGTGGTCAC ACAGTCAGTAAGTGTCAAATCAGACCCAAGAAGGAGGTGTTATTCATTTCTTCATTTACTCAACAACCATTTTCTTGTCGCCTGCTTTGCGCAGGAAGCA CCCCACATGCTGGGGGCACAGTCATGAAGCACACAGACAGGGTCCTGCCTCCAGGGAGTTTATAATTTAGGTGGGGAAAGAACAAGGAAAGTAAAGCATG GACAGATTGTCACAGATAACGACAGTGCCACGCACAAAGCAAAACAGAGCGATGAGATAGAGCGAGCCTGGGAGGGGCAGCTAGGGCCAACGTGCAGAGG CACGGGTGTGGCCTGTCCAGGAACAGCAGGCAGGCGTGCTCCACCCTGACTACATATTCAAATCACCCAGAGAGCTTACAAAGGAAATATCAGTGCTGGG ACCCCAGCCCAGGCCAATGAGGCAGTCAGCTGGGCATTCGTATTTTAAAAATATGCCTTAAGTGATTATACTCTGCAGTCAGAGTTGAAAATGACTGATG TAGGGCTTTGCAGCCATGGAAAGGAGCTTGGATTTTAACACAGTGAAAAGCCTCAGGAGTGTTTCAAGCCAGGGAGCAACATACTTTGATTTGTAGACAG CTGTGTGACCTCGGACAAGTGACTTAATTATTCTGTGCTTCAGTTTCCTCATCTACAAAATGGAGCTAATAATCATGAATTTCCTAGAGTTGTAAGAATG GAATGAGACAGTATCTGTAGTATGCTTAGGACAGTGCCTGGCAGGTGGCATGTGTGATAGGAGTGTTAGCTATTGCAAAGGGAAGGAGGTTTTAACATTT GTACCCTGACTGCTTCGTGGAGAAGGCATTGGGGTGGAAAAGACAAGTTAGGAGACTGATGCTGCAGGAGCCCAGGCCCAGAGCACTCCTGTGTGCTGGG CCTGAGGGATACAGACGGCACAGGCCAGTGCTCCTGTCCCTGATGGGGTGGGAACAGTATTCTAGACCAGGGAACAGTACAGGGGAAAGAGCAC

LGALS9.F5 (forward primer)
LGALS9.R1 (reverse primer)
Multiple exon skipping

Frame 0

CLLNYM*GQGLPTFPELVSSSWAEVILLPQPLK VLVL*A*ATTPCPFF*VGLFYTPVCCE*EFQLLR IAINL*LPTNDVP**NCPWCPRGSQRISLPFSP FCSPCTVYSLNWYLIQEHNGDGQCRWHTWPQSI GASQRN*SANSSASRWSTGRKRGADSRMALGHLP SSRTRTALTCQHLPYPA*VLGLLTSTSLGPGRL QVSLFHGSLPHDLHMPYVPSQPHVT*NVLSCPR LLSSS*SAENKDGEEKEKRIKRDSLRGRIRSSWA PHTVE*KYSLWCFSCKIFPST*LCFYMNNINLN RRNTF*RV*ESS*SSGGHELAV*PGACPLDSEP QLLCM*NGLKPPFPTSTLCTGNAQPHYFLDSVAK HA*DTQPHTSGQCHLATCCHVSVVKHCRPL*TK YASGLG*AQEREEGKVTGAGSAYFPL*VSPQST QYHTFLSLDPLPFAAGSSPLNNSQVALVSISLLS RSLYKGLPSGCERQRWPQRRRRDGLQRFPGSLP ESSCPLFWDYSRRSPGRTSDHCQWDRSQLQWNQ VCCELSDWLQWK*HCLPLQPSV*RWRVRGVQHEA ERKLGARGEEDTHAFPEGDAL*PLLPGAELRFQ GDGERDPLRAVLPPRALPPCGHHLRQWLCAAVL HQLPDPDSHPHSAERPWTDVLYSRHPTYDVPPPR LSDAFHHHHSGRAVPIQVHPPVRHCPAQCSEVP HQPVLWEPHRLPPEPPF**ECCGPQHPDRQLLG V*GAKSAPKNALRPWPELLSKAPQSGAWRGSHGC TGGGGKGGLRYLEQYGG*FLWDEGPKEEKVANK LLLLLLSRMKEVLGSAFSDEETNSQVQVTCLWS HSQ*VSNQTQEGGVIHFFIYSTTIFLSPALRRKH PTCWGHSHEAHRQGPASREFII*VGKEQGK*SM DRLSQITTVPRTKQNRAMR*SEPGRGS*GQRAE ARVWPVQEQQAGVLHPDYIFKSPRELTKEISVLG PQPRPMRQSAGHSYFKNMP*VIILCSQS*K*LM *GFAAMERSLDFNTVKSLRSVSSQGATYFDL*T AV*PRTSDLIILCFSFLIYKMELIIMNFLEL*EW NETVSVVCLGQCLAGGMCDRSVSYCKGKEVLTF VP*LLRGEGIGVEKTS*ETDAAGAQAQSTPVCW A*GIQTAQASAPVPDGVGTVF*TREQYRGKS

Frame 1

VC*IICRDRVFQPFPSWSQAPGLK*SSCLSLSK CWYYRHKPPRPAHFSKLDYFTLQSAVNESSSCC VSQSICDCLQMMCRDEIVPGVREDRKEFPFLSLL SAPLVLSTP*TGT*SRNIMGMDSADGTHGLSLL EHLKGISLQILVHLGGAQEGREVQTHVWPWGTC HPLAPVQPLPASTYPTQLEFWAFSPAHRWDLVDF R*VYFMAPSPTTCTCHMFLPSPMSLEMFYHVQD FSPHPKVQKIRMEKRRRRESREIH*EGGSEAPG HPIL*NKNIHYGVFLAKYSPLHNYVFT*II*I*T GEILFEESENLHKVQAATN*LCDLGHAP*TLSL SFSVCKMG*SRPSPQAPCAQAMPSPIIFWTPWP SMLRTHSHILLGSVIWQLAVMSVWSSIVDLYEPN MLQAWVEHRRGRRERSLGLGVPTSLCECHPSPP STIPFSLWTHFLLLPAPPH*ITAKLLWFLFLC* VVPSTKDFLVGVKGSGGHRGGGEMAFSGSQAPYL SPAVPFSGTIQGGLQDGLQITVNGTVLSSSGTR FAVNFQTGFSGNDIAFHFNPRFEDGGYVVCNTR QNGSWGPEERKTHMPFQKGMPFDLCFLVQSSDFK VMVNGILFVQYFHRVPFHRVDTISVNGSVQLSY ISFQTQTVIHTVQSAPGQMFSTPAIPPMMYPHP AYPMPFITTILGGLYPSKSILLSGTVLPSAQRFH INLCSGNHIAFHLNPRFDENAVVRNTQIDNSWG SEERSLPRKMPFVRGQSFSVRHRSLELGEAPMG AQGEGVKEV*GTLNSMGADSSGMKAQKKRRWPTN YYYYYCPA*RKCWDLLFQMRKQTHKCRLLACGH TVSKCQIRPKKEVLFISSFTQQPFSCRLLCAGS TPHAGGTVMKHTDRVLPPGSL*FRWGKNKESKAW TDCHR*RQCHAQSKTER*DRASLGGAARANVQR HGCGLSRNSRQACSTLTTYSNHPESLQRKYQCW DPSPGQ*GSQLGIRILKICLK*LYSAVRVEND*C RALQPWKGAWILTQ*KASGVFQAREQHTLICRQ LCDLGQVT*LFCASVSSSTKWS**S*IS*SCKN GMRQYL*YA*DSAWQVACVIGVLAIAKGRRF*HL YPDCFVEKALGWKRQVRRLMLQEPRPRALLCAG PEGYRRHRPVLLSLMGWEQYSRPGNSTGERA

Frame 2

FVKLYVGTGSSNLSRAGLKLLG*SDPPASASQS AGIIGISHHALPIFLSWIILHSSLL*MRVPVVA YRNQFVIAYK*CAVMKLSLVSERIAKNFPSFLS FLLPLYCLLLKLVLDPGT*WGWTVQMAHMASVYW SISKELVCKF*CI*VEHRKEERCRLTYGPGAPA ILSHPYSPYLPALTLPSLSSGPSHQHIAGTWST SGKFISWLPPPRPAHAICSFPAPCHLKCSIMSKT SLLILKCRK*GWRREGEENQERFIEREDQKLLG TPYCRIKIFIMVFFLQNIPLYIIMFLHE*YKFK QEKYFLKSLRIFIKFRRPRISCVTWGMPLRL*AS ASLYVKWVEAALPHKHPVHRQCPAPLFSGLRGQ ACLGHTATYFWAVSSGNLLSCQCGQAL*TSMNQ ICFRLGLSTGEGGGKGHWGWECLLPSVSVTPVHP VPYLSLSGPTSFCCRLLPIE*QPSCFGFYFFVK SFPLQRTS*WV*KAAVATEAAERWPSAVPRLPT *VQLSPFLGLFKEVSRTDFRSLSMGPFSAPVEPG LL*TFRLASVEMTLPSTSTLGLKMEGTWCATRG RTEAGGPRRGRHTCLSRRGCPLTSASWCRAQIS R*W*TGSSSCSTSTACPSTVWTPSPSMALCSCPT SASRPRQSSTQCRAPLDRCSLLPPSHL*CTPTP PIRCLSSPPFWEGCTHPSPSSCQALSCPVLRGS TSTCALGTTSPST*TPVLMRMLWSATPRSTTPGG LRSEVCPEKCPSSVARASQ*GTAVWSLERLPWV HRGRG*RRFEVP*TVWGLIPLG*RPKRREGGQQ IIIIITVPHEGSAGICFFR*GNKLTSAGYLLVVT QSVSVKSDPRRRCYSFLHLLNNHFLVACFAQEA PHMLGAQS*STQTGSCLQGVYNLGGERTRKVKH GQIVTDNDSATHKAKQSDEIERAWEGQLGPTCRG TGVACPGTAGRRAPP*LHIQITQRAYKGNISAG TPAQANEAVSWAFVF*KYALSDYTLQSELKMTD VGLCSHGKELGF*HSEKPQECFKPGSNIL*FVDS CVTSDK*LNYSVLQFPHLQNGANNHEFPRVVRM E*DSICSMLRTVPGRWHV**EC*LLQREGGFNI CTLTASWRRHWGGKDKLGD*CCRSPGPEHSCVLG LRDTDGTGQCSCP*WGGNSILDQGTVQGKEH

Sequence LGALS9.eAug05

TGTTTGTTAAATTATATGTAGGGACAGGGTCTTCCAACCTTTCCCGAGCTGGTCTCAAGCTCCTGGGCTGAAGTGATCCTCCTGCCTCAGCCTCTCAAAG TGCTGGTATTATAGGCATAAGCCACCACGCCCTGCCCATTTTTCTAAGTTGGATTATTTTACACTCCAGTCTGCTGTGAATGAGAGTTCCAGTTGTTGCG TATCGCAATCAATTTGTGATTGCCTACAAATGATGTGCCGTGATGAAATTGTCCCTGGTGTCCGAGAGGATCGCAAAGAATTTCCCTTCCTTTCTCTCCT TTCTGCTCCCCTTGTACTGTCTACTCCTTAAACTGGTACTTGATCCAGGAACATAATGGGGATGGACAGTGCAGATGGCACACATGGCCTCAGTCTATTG GAGCATCTCAAAGGAATTAGTCTGCAAATTCTAGTGCATCTAGGTGGAGCACAGGAAGGAAGAGAGGTGCAGACTCACGTATGGCCCTGGGGCACCTGCC ATCCTCTCGCACCCGTACAGCCCTTACCTGCCAGCACTTACCCTACCCAGCTTGAGTTCTGGGCCTTCTCACCAGCACATCGCTGGGACCTGGTCGACTT CAGGTAAGTTTATTTCATGGCTCCCTCCCCCACGACCTGCACATGCCATATGTTCCTTCCCAGCCCCATGTCACTTGAAATGTTCTATCATGTCCAAGAC TTCTCTCCTCATCCTAAAGTGCAGAAAATAAGGATGGAGAAGAGAAGGAGAAGAGAATCAAGAGAGATTCATTGAGAGGGAGGATCAGAAGCTCCTGGGC ACCCCATACTGTAGAATAAAAATATTCATTATGGTGTTTTTCTTGCAAAATATTCCCCTCTACATAATTATGTTTTTACATGAATAATATAAATTTAAAC AGGAGAAATACTTTTTGAAGAGTCTGAGAATCTTCATAAAGTTCAGGCGGCCACGAATTAGCTGTGTGACCTGGGGCATGCCCCTTAGACTCTGAGCCTC AGCTTCTCTGTATGTAAAATGGGTTGAAGCCGCCCTTCCCCACAAGCACCCTGTGCACAGGCAATGCCCAGCCCCATTATTTTCTGGACTCCGTGGCCAA GCATGCTTAGGACACACAGCCACATACTTCTGGGCAGTGTCATCTGGCAACTTGCTGTCATGTCAGTGTGGTCAAGCATTGTAGACCTCTATGAACCAAA TATGCTTCAGGCTTGGGTTGAGCACAGGAGAGGGAGGAGGGAAAGGTCACTGGGGCTGGGAGTGCCTACTTCCCTCTGTGAGTGTCACCCCAGTCCACCC AGTACCATACCTTTCTCTCTCTGGACCCACTTCCTTTTGCTGCCGGCTCCTCCCCATTGAATAACAGCCAAGTTGCTTTGGTTTCTATTTCTTTGTTAAG TCGTTCCCTCTACAAAGGACTTCCTAGTGGGTGTGAAAGGCAGCGGTGGCCACAGAGGCGGCGGAGAGATGGCCTTCAGCGGTTCCCAGGCTCCCTACCT GAGTCCAGCTGTCCCCTTTTCTGGGACTATTCAAGGAGGTCTCCAGGACGGACTTCAGATCACTGTCAATGGGACCGTTCTCAGCTCCAGTGGAACCAGG TTTGCTGTGAACTTTCAGACTGGCTTCAGTGGAAATGACATTGCCTTCCACTTCAACCCTCGGTTTGAAGATGGAGGGTACGTGGTGTGCAACACGAGGC AGAACGGAAGCTGGGGGCCCGAGGAGAGGAAGACACACATGCCTTTCCAGAAGGGGATGCCCTTTGACCTCTGCTTCCTGGTGCAGAGCTCAGATTTCAA GGTGATGGTGAACGGGATCCTCTTCGTGCAGTACTTCCACCGCGTGCCCTTCCACCGTGTGGACACCATCTCCGTCAATGGCTCTGTGCAGCTGTCCTAC ATCAGCTTCCAGACCCAGACAGTCATCCACACAGTGCAGAGCGCCCCTGGACAGATGTTCTCTACTCCCGCCATCCCACCTATGATGTACCCCCACCCCG CCTATCCGATGCCTTTCATCACCACCATTCTGGGAGGGCTGTACCCATCCAAGTCCATCCTCCTGTCAGGCACTGTCCTGCCCAGTGCTCAGAGGTGTGG ATCTTGTGTGAAGCTCACTGCCTCAAGGTGGCCGTGGATGGTCAGCACCTGTTTGAATACTACCATCGCCTGAGGAACCTGCCCACCATCAACAGACTGG AAGTGGGGGGCGACATCCAGCTGACCCATGTGCAGACATAGGCGGCTTCCTGGCCCTGGGGCCGGGGGCTGGGGTGTGGGGCAGTCTGGGTCCTCTCATC ATCCCCACTTCCCAGGCCCAGCCTTTCCAACCCTGCCTGGGATCTGGGCTTTAATGCAGAGGCCATGTCCTTGTCTGGTCCTGCTTCTGGCTACAGCCAC CCTGGAACGGAGAAGGCAGCTGACGGGGATTGCCTTCCTCAGCCGCAGCAGCACCTGGGGCTCCAGCTGCTGGAATCCTACCATCCCAGGAGGCAGGCAC AGCCAGGGAGAGGGGAGGAGTGGGCAGTGAAGATGAAGCCCCATGCTCAGTCCCCTCCCATCCCCCACGCAGCTCCACCCCAGTCCCAAGCCACCAGCTG TCTGCTCCTGGTGGGAGGTGGCCTCCTCAGCCCCTCCTCTCTGACCTTTAACCTCACTCTCACCTTGCACCGTGCACCAACCCTTCACCCCTCCTGGAAA GCAGGCCTGATGGCTTCCCACTGGCCTCCACCACCTGACCAGAGTGTTCTCTTCAGAGGACTGGCTCCTTTCCCAGTGTCCTTAAAATAAAGAAATGAAA ATGCTTGTTGGCACATTCA

LGALS9.F5 (forward primer)
LGALS9.R1 (reverse primer)
Multiple exon skipping

Frame 0

CLLNYM*GQGLPTFPELVSSSWAEVILLPQPLK VLVL*A*ATTPCPFF*VGLFYTPVCCE*EFQLLR IAINL*LPTNDVP**NCPWCPRGSQRISLPFSP FCSPCTVYSLNWYLIQEHNGDGQCRWHTWPQSI GASQRN*SANSSASRWSTGRKRGADSRMALGHLP SSRTRTALTCQHLPYPA*VLGLLTSTSLGPGRL QVSLFHGSLPHDLHMPYVPSQPHVT*NVLSCPR LLSSS*SAENKDGEEKEKRIKRDSLRGRIRSSWA PHTVE*KYSLWCFSCKIFPST*LCFYMNNINLN RRNTF*RV*ESS*SSGGHELAV*PGACPLDSEP QLLCM*NGLKPPFPTSTLCTGNAQPHYFLDSVAK HA*DTQPHTSGQCHLATCCHVSVVKHCRPL*TK YASGLG*AQEREEGKVTGAGSAYFPL*VSPQST QYHTFLSLDPLPFAAGSSPLNNSQVALVSISLLS RSLYKGLPSGCERQRWPQRRRRDGLQRFPGSLP ESSCPLFWDYSRRSPGRTSDHCQWDRSQLQWNQ VCCELSDWLQWK*HCLPLQPSV*RWRVRGVQHEA ERKLGARGEEDTHAFPEGDAL*PLLPGAELRFQ GDGERDPLRAVLPPRALPPCGHHLRQWLCAAVL HQLPDPDSHPHSAERPWTDVLYSRHPTYDVPPPR LSDAFHHHHSGRAVPIQVHPPVRHCPAQCSEVW ILCEAHCLKVAVDGQHLFEYYHRLRNLPTINRL EVGGDIQLTHVQT*AASWPWGRGLGCGAVWVLSS SPLPRPSLSNPAWDLGFNAEAMSLSGPASGYSH PGTEKAADGDCLPQPQQHLGLQLLESYHPRRQA QPGRGEEWAVKMKPHAQSPPIPHAAPPQSQATSC LLLVGGGLLSPSSLTFNLTLTLHRAPTLHPSWK AGLMASHWPPPPDQSVLFRGLAPFPVSLK*RNE NACWHI

Frame 1

VC*IICRDRVFQPFPSWSQAPGLK*SSCLSLSK CWYYRHKPPRPAHFSKLDYFTLQSAVNESSSCC VSQSICDCLQMMCRDEIVPGVREDRKEFPFLSLL SAPLVLSTP*TGT*SRNIMGMDSADGTHGLSLL EHLKGISLQILVHLGGAQEGREVQTHVWPWGTC HPLAPVQPLPASTYPTQLEFWAFSPAHRWDLVDF R*VYFMAPSPTTCTCHMFLPSPMSLEMFYHVQD FSPHPKVQKIRMEKRRRRESREIH*EGGSEAPG HPIL*NKNIHYGVFLAKYSPLHNYVFT*II*I*T GEILFEESENLHKVQAATN*LCDLGHAP*TLSL SFSVCKMG*SRPSPQAPCAQAMPSPIIFWTPWP SMLRTHSHILLGSVIWQLAVMSVWSSIVDLYEPN MLQAWVEHRRGRRERSLGLGVPTSLCECHPSPP STIPFSLWTHFLLLPAPPH*ITAKLLWFLFLC* VVPSTKDFLVGVKGSGGHRGGGEMAFSGSQAPYL SPAVPFSGTIQGGLQDGLQITVNGTVLSSSGTR FAVNFQTGFSGNDIAFHFNPRFEDGGYVVCNTR QNGSWGPEERKTHMPFQKGMPFDLCFLVQSSDFK VMVNGILFVQYFHRVPFHRVDTISVNGSVQLSY ISFQTQTVIHTVQSAPGQMFSTPAIPPMMYPHP AYPMPFITTILGGLYPSKSILLSGTVLPSAQRCG SCVKLTASRWPWMVSTCLNTTIA*GTCPPSTDW KWGATSS*PMCRHRRLPGPGAGGWGVGQSGSSH HPHFPGPAFPTLPGIWALMQRPCPCLVLLLATAT LERRRQLTGIAFLSRSSTWGSSCWNPTIPGGRH SQGEGRSGQ*R*SPMLSPLPSPTQLHPSPKPPA VCSWWEVASSAPPL*PLTSLSPCTVHQPFTPPGK QA*WLPTGLHHLTRVFSSEDWLLSQCP*NKEMK MLVGTF

Frame 2

FVKLYVGTGSSNLSRAGLKLLG*SDPPASASQS AGIIGISHHALPIFLSWIILHSSLL*MRVPVVA YRNQFVIAYK*CAVMKLSLVSERIAKNFPSFLS FLLPLYCLLLKLVLDPGT*WGWTVQMAHMASVYW SISKELVCKF*CI*VEHRKEERCRLTYGPGAPA ILSHPYSPYLPALTLPSLSSGPSHQHIAGTWST SGKFISWLPPPRPAHAICSFPAPCHLKCSIMSKT SLLILKCRK*GWRREGEENQERFIEREDQKLLG TPYCRIKIFIMVFFLQNIPLYIIMFLHE*YKFK QEKYFLKSLRIFIKFRRPRISCVTWGMPLRL*AS ASLYVKWVEAALPHKHPVHRQCPAPLFSGLRGQ ACLGHTATYFWAVSSGNLLSCQCGQAL*TSMNQ ICFRLGLSTGEGGGKGHWGWECLLPSVSVTPVHP VPYLSLSGPTSFCCRLLPIE*QPSCFGFYFFVK SFPLQRTS*WV*KAAVATEAAERWPSAVPRLPT *VQLSPFLGLFKEVSRTDFRSLSMGPFSAPVEPG LL*TFRLASVEMTLPSTSTLGLKMEGTWCATRG RTEAGGPRRGRHTCLSRRGCPLTSASWCRAQIS R*W*TGSSSCSTSTACPSTVWTPSPSMALCSCPT SASRPRQSSTQCRAPLDRCSLLPPSHL*CTPTP PIRCLSSPPFWEGCTHPSPSSCQALSCPVLRGV DLV*SSLPQGGRGWSAPV*ILPSPEEPAHHQQTG SGGRHPADPCADIGGFLALGPGAGVWGSLGPLI IPTSQAQPFQPCLGSGL*CRGHVLVWSCFWLQP PWNGEGS*RGLPSSAAAAPGAPAAGILPSQEAGT ARERGGVGSEDEAPCSVPSHPPRSSTPVPSHQL SAPGGRWPPQPLLSDL*PHSHLAPCTNPSPLLE SRPDGFPLASTT*PECSLQRTGSFPSVLKIKK*K CLLAHS

Sequence LGALS9.iAug05

TGTTTGTTAAATTATATGTAGGGACAGGGTCTTCCAACCTTTCCCGAGCTGGTCTCAAGCTCCTGGGCTGAAGTGATCCTCCTGCCTCAGCCTCTCAAAG TGCTGGTATTATAGGCATAAGCCACCACGCCCTGCCCATTTTTCTAAGTTGGATTATTTTACACTCCAGTCTGCTGTGAATGAGAGTTCCAGTTGTTGCG TATCGCAATCAATTTGTGATTGCCTACAAATGATGTGCCGTGATGAAATTGTCCCTGGTGTCCGAGAGGATCGCAAAGAATTTCCCTTCCTTTCTCTCCT TTCTGCTCCCCTTGTACTGTCTACTCCTTAAACTGGTACTTGATCCAGGAACATAATGGGGATGGACAGTGCAGATGGCACACATGGCCTCAGTCTATTG GAGCATCTCAAAGGAATTAGTCTGCAAATTCTAGTGCATCTAGGTGGAGCACAGGAAGGAAGAGAGGTGCAGACTCACGTATGGCCCTGGGGCACCTGCC ATCCTCTCGCACCCGTACAGCCCTTACCTGCCAGCACTTACCCTACCCAGCTTGAGTTCTGGGCCTTCTCACCAGCACATCGCTGGGACCTGGTCGACTT CAGGTAAGTTTATTTCATGGCTCCCTCCCCCACGACCTGCACATGCCATATGTTCCTTCCCAGCCCCATGTCACTTGAAATGTTCTATCATGTCCAAGAC TTCTCTCCTCATCCTAAAGTGCAGAAAATAAGGATGGAGAAGAGAAGGAGAAGAGAATCAAGAGAGATTCATTGAGAGGGAGGATCAGAAGCTCCTGGGC ACCCCATACTGTAGAATAAAAATATTCATTATGGTGTTTTTCTTGCAAAATATTCCCCTCTACATAATTATGTTTTTACATGAATAATATAAATTTAAAC AGGAGAAATACTTTTTGAAGAGTCTGAGAATCTTCATAAAGTTCAGGCGGCCACGAATTAGCTGTGTGACCTGGGGCATGCCCCTTAGACTCTGAGCCTC AGCTTCTCTGTATGTAAAATGGGTTGAAGCCGCCCTTCCCCACAAGCACCCTGTGCACAGGCAATGCCCAGCCCCATTATTTTCTGGACTCCGTGGCCAA GCATGCTTAGGACACACAGCCACATACTTCTGGGCAGTGTCATCTGGCAACTTGCTGTCATGTCAGTGTGGTCAAGCATTGTAGACCTCTATGAACCAAA TATGCTTCAGGCTTGGGTTGAGCACAGGAGAGGGAGGAGGGAAAGGTCACTGGGGCTGGGAGTGCCTACTTCCCTCTGTGAGTGTCACCCCAGTCCACCC AGTACCATACCTTTCTCTCTCTGGACCCACTTCCTTTTGCTGCCGGCTCCTCCCCATTGAATAACAGCCAAGTTGCTTTGGTTTCTATTTCTTTGTTAAG TCGTTCCCTCTACAAAGGACTTCCTAGTGGGTGTGAAAGGCAGCGGTGGCCACAGAGGCGGCGGAGAGATGGCCTTCAGCGGTTCCCAGGCTCCCTACCT GAGTCCAGCTGTCCCCTTTTCTGGGACTATTCAAGGAGGTCTCCAGGACGGACTTCAGATCACTGTCAATGGGACCGTTCTCAGCTCCAGTGGAACCAGA TGGAGGGTACGTGGTGTGCAACACGAGGCAGAACGGAAGCTGGGGGCCCGAGGAGAGGAAGACACACATGCCTTTCCAGAAGGGGATGCCCTTTGACCTC TGCTTCCTGGTGCAGAGCTCAGATTTCAAGGTGATGGTGAACGGGATCCTCTTCGTGCAGTACTTCCACCGCGTGCCCTTCCACCGTGTGGACACCATCT CCGTCAATGGCTCTGTGCAGCTGTCCTACATCAGCTTCCAGAACCCCCGCACAGTCCCTGTTCAGCCTGCCTTCTCCACGGTGCCGTTCTCCCAGCCTGT CTGTTTCCCACCCAGGCCCAGGGGGCGCAGACAAAAACCTCCCGGCGTGTGGCCTGCCAACCCGGCTCCCATTACCCAGACAGTCATCCACACAGTGCAG AGCGCCCCTGGACAGATGTTCTCTACTCCCGCCATCCCACCTATGATGTACCCCCACCCCGCCTATCCGATGCCTTTCATCACCACCATTCTGGGAGGGC TGTACCCATCCAAGTCCATCCTCCTGTCAGGCACTGTCCTGCCCAGTGCTCAGAGGTAAGCCAAGGGCTCCAGTGACCTCTGGGAAGAGAGAGCCCTTCA AGGTCAGTCCAGCCATTCCCCTGGCTTCAGGAAGGCTACTGATGATGGGGAGGAAATGGGACTCAGAATTCGGTGGATAAAGGTTCAGGTGGGCTGCCCA CCCCAGGTTCCACATCAACCTGTGCTCTGGGAACCACATCGCCTTCCACCTGAACCCCCGTTTTGATGAGAATGCTGTGGTCCGCAACACCCAGATCGAC AACTCCTGGGGGTCTGAGGAGCGAAGTCTGCCCCGAAAAATGCCCTTCGTCCGTGGCCAGAGCTTCTCAGTAAGGCACCGCAGTCTGGAGCTTGGAGAGG CTCCCATGGGTGCACAGGGGGAGGGGGTAAAGGAGGTTTGAGGTACCTTGAACAGTATGGGGGCTGATTCCTCTGGGATGAAGGCCCAAAAGAAGAGAAG GTGGCCAACAAATTATTATTATTATTACTGTCCCGCATGAAGGAAGTGCTGGGATCTGCTTTTTCAGATGAGGAAACAAACTCACAAGTGCAGGTTACTT GCTTGTGGTCACACAGTCAGTAAGTGTCAAATCAGACCCAAGAAGGAGGTGTTATTCATTTCTTCATTTACTCAACAACCATTTTCTTGTCGCCTGCTTT GCGCAGGAAGCACCCCACATGCTGGGGGCACAGTCATGAAGCACACAGACAGGGTCCTGCCTCCAGGGAGTTTATAATTTAGGTGGGGAAAGAACAAGGA AAGTAAAGCATGGACAGATTGTCACAGATAACGACAGTGCCACGCACAAAGCAAAACAGAGCGATGAGATAGAGCGAGCCTGGGAGGGGCAGCTAGGGCC AACGTGCAGAGGCACGGGTGTGGCCTGTCCAGGAACAGCAGGCAGGCGTGCTCCACCCTGACTACATATTCAAATCACCCAGAGAGCTTACAAAGGAAAT ATCAGTGCTGGGACCCCAGCCCAGGCCAATGAGGCAGTCAGCTGGGCATTCGTATTTTAAAAATATGCCTTAAGTGATTATACTCTGCAGTCAGAGTTGA AAATGACTGATGTAGGGCTTTGCAGCCATGGAAAGGAGCTTGGATTTTAACACAGTGAAAAGCCTCAGGAGTGTTTCAAGCCAGGGAGCAACATACTTTG ATTTGTAGACAGCTGTGTGACCTCGGACAAGTGACTTAATTATTCTGTGCTTCAGTTTCCTCATCTACAAAATGGAGCTAATAATCATGAATTTCCTAGA GTTGTAAGAATGGAATGAGACAGTATCTGTAGTATGCTTAGGACAGTGCCTGGCAGGTGGCATGTGTGATAGGAGTGTTAGCTATTGCAAAGGGAAGGAG GTTTTAACATTTGTACCCTGACTGCTTCGTGGAGAAGGCATTGGGGTGGAAAAGACAAGTTAGGAGACTGATGCTGCAGGAGCCCAGGCCCAGAGCACTC CTGTGTGCTGGGCCTGAGGGATACAGACGGCACAGGCCAGTGCTCCTGTCCCTGATGGGGTGGGAACAGTATTCTAGACCAGGGAACAGTACAGGGGAAA GAGCAC

LGALS9.F5 (forward primer)
LGALS9.R1 (reverse primer)
Multiple exon skipping

Frame 0

CLLNYM*GQGLPTFPELVSSSWAEVILLPQPLK VLVL*A*ATTPCPFF*VGLFYTPVCCE*EFQLLR IAINL*LPTNDVP**NCPWCPRGSQRISLPFSP FCSPCTVYSLNWYLIQEHNGDGQCRWHTWPQSI GASQRN*SANSSASRWSTGRKRGADSRMALGHLP SSRTRTALTCQHLPYPA*VLGLLTSTSLGPGRL QVSLFHGSLPHDLHMPYVPSQPHVT*NVLSCPR LLSSS*SAENKDGEEKEKRIKRDSLRGRIRSSWA PHTVE*KYSLWCFSCKIFPST*LCFYMNNINLN RRNTF*RV*ESS*SSGGHELAV*PGACPLDSEP QLLCM*NGLKPPFPTSTLCTGNAQPHYFLDSVAK HA*DTQPHTSGQCHLATCCHVSVVKHCRPL*TK YASGLG*AQEREEGKVTGAGSAYFPL*VSPQST QYHTFLSLDPLPFAAGSSPLNNSQVALVSISLLS RSLYKGLPSGCERQRWPQRRRRDGLQRFPGSLP ESSCPLFWDYSRRSPGRTSDHCQWDRSQLQWNQ MEGTWCATRGRTEAGGPRRGRHTCLSRRGCPLTS ASWCRAQISR*W*TGSSSCSTSTACPSTVWTPS PSMALCSCPTSASRTPAQSLFSLPSPRCRSPSL SVSHPGPGGADKNLPACGLPTRLPLPRQSSTQCR APLDRCSLLPPSHL*CTPTPPIRCLSSPPFWEG CTHPSPSSCQALSCPVLRGKPRAPVTSGKREPF KVSPAIPLASGRLLMMGRKWDSEFGG*RFRWAAH PRFHINLCSGNHIAFHLNPRFDENAVVRNTQID NSWGSEERSLPRKMPFVRGQSFSVRHRSLELGE APMGAQGEGVKEV*GTLNSMGADSSGMKAQKKRR WPTNYYYYYCPA*RKCWDLLFQMRKQTHKCRLL ACGHTVSKCQIRPKKEVLFISSFTQQPFSCRLL CAGSTPHAGGTVMKHTDRVLPPGSL*FRWGKNKE SKAWTDCHR*RQCHAQSKTER*DRASLGGAARA NVQRHGCGLSRNSRQACSTLTTYSNHPESLQRK YQCWDPSPGQ*GSQLGIRILKICLK*LYSAVRVE ND*CRALQPWKGAWILTQ*KASGVFQAREQHTL ICRQLCDLGQVT*LFCASVSSSTKWS**S*IS* SCKNGMRQYL*YA*DSAWQVACVIGVLAIAKGRR F*HLYPDCFVEKALGWKRQVRRLMLQEPRPRAL LCAGPEGYRRHRPVLLSLMGWEQYSRPGNSTGE RA

Frame 1

VC*IICRDRVFQPFPSWSQAPGLK*SSCLSLSK CWYYRHKPPRPAHFSKLDYFTLQSAVNESSSCC VSQSICDCLQMMCRDEIVPGVREDRKEFPFLSLL SAPLVLSTP*TGT*SRNIMGMDSADGTHGLSLL EHLKGISLQILVHLGGAQEGREVQTHVWPWGTC HPLAPVQPLPASTYPTQLEFWAFSPAHRWDLVDF R*VYFMAPSPTTCTCHMFLPSPMSLEMFYHVQD FSPHPKVQKIRMEKRRRRESREIH*EGGSEAPG HPIL*NKNIHYGVFLAKYSPLHNYVFT*II*I*T GEILFEESENLHKVQAATN*LCDLGHAP*TLSL SFSVCKMG*SRPSPQAPCAQAMPSPIIFWTPWP SMLRTHSHILLGSVIWQLAVMSVWSSIVDLYEPN MLQAWVEHRRGRRERSLGLGVPTSLCECHPSPP STIPFSLWTHFLLLPAPPH*ITAKLLWFLFLC* VVPSTKDFLVGVKGSGGHRGGGEMAFSGSQAPYL SPAVPFSGTIQGGLQDGLQITVNGTVLSSSGTR WRVRGVQHEAERKLGARGEEDTHAFPEGDAL*P LLPGAELRFQGDGERDPLRAVLPPRALPPCGHHL RQWLCAAVLHQLPEPPHSPCSACLLHGAVLPAC LFPTQAQGAQTKTSRRVACQPGSHYPDSHPHSA ERPWTDVLYSRHPTYDVPPPRLSDAFHHHHSGRA VPIQVHPPVRHCPAQCSEVSQGLQ*PLGRESPS RSVQPFPWLQEGY**WGGNGTQNSVDKGSGGLP TPGSTSTCALGTTSPST*TPVLMRMLWSATPRST TPGGLRSEVCPEKCPSSVARASQ*GTAVWSLER LPWVHRGRG*RRFEVP*TVWGLIPLG*RPKRRE GGQQIIIIITVPHEGSAGICFFR*GNKLTSAGYL LVVTQSVSVKSDPRRRCYSFLHLLNNHFLVACF AQEAPHMLGAQS*STQTGSCLQGVYNLGGERTR KVKHGQIVTDNDSATHKAKQSDEIERAWEGQLGP TCRGTGVACPGTAGRRAPP*LHIQITQRAYKGN ISAGTPAQANEAVSWAFVF*KYALSDYTLQSEL KMTDVGLCSHGKELGF*HSEKPQECFKPGSNIL* FVDSCVTSDK*LNYSVLQFPHLQNGANNHEFPR VVRME*DSICSMLRTVPGRWHV**EC*LLQREG GFNICTLTASWRRHWGGKDKLGD*CCRSPGPEHS CVLGLRDTDGTGQCSCP*WGGNSILDQGTVQGK EH

Frame 2

FVKLYVGTGSSNLSRAGLKLLG*SDPPASASQS AGIIGISHHALPIFLSWIILHSSLL*MRVPVVA YRNQFVIAYK*CAVMKLSLVSERIAKNFPSFLS FLLPLYCLLLKLVLDPGT*WGWTVQMAHMASVYW SISKELVCKF*CI*VEHRKEERCRLTYGPGAPA ILSHPYSPYLPALTLPSLSSGPSHQHIAGTWST SGKFISWLPPPRPAHAICSFPAPCHLKCSIMSKT SLLILKCRK*GWRREGEENQERFIEREDQKLLG TPYCRIKIFIMVFFLQNIPLYIIMFLHE*YKFK QEKYFLKSLRIFIKFRRPRISCVTWGMPLRL*AS ASLYVKWVEAALPHKHPVHRQCPAPLFSGLRGQ ACLGHTATYFWAVSSGNLLSCQCGQAL*TSMNQ ICFRLGLSTGEGGGKGHWGWECLLPSVSVTPVHP VPYLSLSGPTSFCCRLLPIE*QPSCFGFYFFVK SFPLQRTS*WV*KAAVATEAAERWPSAVPRLPT *VQLSPFLGLFKEVSRTDFRSLSMGPFSAPVEPD GGYVVCNTRQNGSWGPEERKTHMPFQKGMPFDL CFLVQSSDFKVMVNGILFVQYFHRVPFHRVDTI SVNGSVQLSYISFQNPRTVPVQPAFSTVPFSQPV CFPPRPRGRRQKPPGVWPANPAPITQTVIHTVQ SAPGQMFSTPAIPPMMYPHPAYPMPFITTILGG LYPSKSILLSGTVLPSAQR*AKGSSDLWEERALQ GQSSHSPGFRKATDDGEEMGLRIRWIKVQVGCP PQVPHQPVLWEPHRLPPEPPF**ECCGPQHPDR QLLGV*GAKSAPKNALRPWPELLSKAPQSGAWRG SHGCTGGGGKGGLRYLEQYGG*FLWDEGPKEEK VANKLLLLLLSRMKEVLGSAFSDEETNSQVQVT CLWSHSQ*VSNQTQEGGVIHFFIYSTTIFLSPAL RRKHPTCWGHSHEAHRQGPASREFII*VGKEQG K*SMDRLSQITTVPRTKQNRAMR*SEPGRGS*G QRAEARVWPVQEQQAGVLHPDYIFKSPRELTKEI SVLGPQPRPMRQSAGHSYFKNMP*VIILCSQS* K*LM*GFAAMERSLDFNTVKSLRSVSSQGATYF DL*TAV*PRTSDLIILCFSFLIYKMELIIMNFLE L*EWNETVSVVCLGQCLAGGMCDRSVSYCKGKE VLTFVP*LLRGEGIGVEKTS*ETDAAGAQAQST PVCWA*GIQTAQASAPVPDGVGTVF*TREQYRGK S

Sequence LGALS9.aAug05

TGTTTGTTAAATTATATGTAGGGACAGGGTCTTCCAACCTTTCCCGAGCTGGTCTCAAGCTCCTGGGCTGAAGTGATCCTCCTGCCTCAGCCTCTCAAAG TGCTGGTATTATAGGCATAAGCCACCACGCCCTGCCCATTTTTCTAAGTTGGATTATTTTACACTCCAGTCTGCTGTGAATGAGAGTTCCAGTTGTTGCG TATCGCAATCAATTTGTGATTGCCTACAAATGATGTGCCGTGATGAAATTGTCCCTGGTGTCCGAGAGGATCGCAAAGAATTTCCCTTCCTTTCTCTCCT TTCTGCTCCCCTTGTACTGTCTACTCCTTAAACTGGTACTTGATCCAGGAACATAATGGGGATGGACAGTGCAGATGGCACACATGGCCTCAGTCTATTG GAGCATCTCAAAGGAATTAGTCTGCAAATTCTAGTGCATCTAGGTGGAGCACAGGAAGGAAGAGAGGTGCAGACTCACGTATGGCCCTGGGGCACCTGCC ATCCTCTCGCACCCGTACAGCCCTTACCTGCCAGCACTTACCCTACCCAGCTTGAGTTCTGGGCCTTCTCACCAGCACATCGCTGGGACCTGGTCGACTT CAGGTAAGTTTATTTCATGGCTCCCTCCCCCACGACCTGCACATGCCATATGTTCCTTCCCAGCCCCATGTCACTTGAAATGTTCTATCATGTCCAAGAC TTCTCTCCTCATCCTAAAGTGCAGAAAATAAGGATGGAGAAGAGAAGGAGAAGAGAATCAAGAGAGATTCATTGAGAGGGAGGATCAGAAGCTCCTGGGC ACCCCATACTGTAGAATAAAAATATTCATTATGGTGTTTTTCTTGCAAAATATTCCCCTCTACATAATTATGTTTTTACATGAATAATATAAATTTAAAC AGGAGAAATACTTTTTGAAGAGTCTGAGAATCTTCATAAAGTTCAGGCGGCCACGAATTAGCTGTGTGACCTGGGGCATGCCCCTTAGACTCTGAGCCTC AGCTTCTCTGTATGTAAAATGGGTTGAAGCCGCCCTTCCCCACAAGCACCCTGTGCACAGGCAATGCCCAGCCCCATTATTTTCTGGACTCCGTGGCCAA GCATGCTTAGGACACACAGCCACATACTTCTGGGCAGTGTCATCTGGCAACTTGCTGTCATGTCAGTGTGGTCAAGCATTGTAGACCTCTATGAACCAAA TATGCTTCAGGCTTGGGTTGAGCACAGGAGAGGGAGGAGGGAAAGGTCACTGGGGCTGGGAGTGCCTACTTCCCTCTGTGAGTGTCACCCCAGTCCACCC AGTACCATACCTTTCTCTCTCTGGACCCACTTCCTTTTGCTGCCGGCTCCTCCCCATTGAATAACAGCCAAGTTGCTTTGGTTTCTATTTCTTTGTTAAG TCGTTCCCTCTACAAAGGACTTCCTAGTGGGTGTGAAAGGCAGCGGTGGCCACAGAGGCGGCGGAGAGATGGCCTTCAGCGGTTCCCAGGCTCCCTACCT GAGTCCAGCTGTCCCCTTTTCTGGGACTATTCAAGGAGGTCTCCAGGACGGACTTCAGATCACTGTCAATGGGACCGTTCTCAGCTCCAGTGGAACCAGG TTTGCTGTGAACTTTCAGACTGGCTTCAGTGGAAATGACATTGCCTTCCACTTCAACCCTCGGTTTGAAGATGGAGGGTACGTGGTGTGCAACACGAGGC AGAACGGAAGCTGGGGGCCCGAGGAGAGGAAGACACACATGCCTTTCCAGAAGGGGATGCCCTTTGACCTCTGCTTCCTGGTGCAGAGCTCAGATTTCAA GGTGATGGTGAACGGGATCCTCTTCGTGCAGTACTTCCACCGCGTGCCCTTCCACCGTGTGGACACCATCTCCGTCAATGGCTCTGTGCAGCTGTCCTAC ATCAGCTTCCAGAACCCCCGCACAGTCCCTGTTCAGCCTGCCTTCTCCACGGTGCCGTTCTCCCAGCCTGTCTGTTTCCCACCCAGGCCCAGGGGGCGCA GACAAAAACCTCCCGGCGTGTGGCCTGCCAACCCGGCTCCCATTACCCAGACAGTCATCCACACAGTGCAGAGCGCCCCTGGACAGATGTTCTCTACTCC CGCCATCCCACCTATGATGTACCCCCACCCCGCCTATCCGATGCCTTTCATCACCACCATTCTGGGAGGGCTGTACCCATCCAAGTCCATCCTCCTGTCA GGCACTGTCCTGCCCAGTGCTCAGAGGTTCCACATCAACCTGTGCTCTGGGAACCACATCGCCTTCCACCTGAACCCCCGTTTTGATGAGAATGCTGTGG TCCGCAACACCCAGATCGACAACTCCTGGGGGTCTGAGGAGCGAAGTCTGCCCCGAAAAATGCCCTTCGTCCGTGGCCAGAGCTTCTCAGTGTGGATCTT GTGTGAAGCTCACTGCCTCAAGGTGGCCGTGGATGGTCAGCACCTGTTTGAATACTACCATCGCCTGAGGAACCTGCCCACCATCAACAGACTGGAAGTG GGGGGCGACATCCAGCTGACCCATGTGCAGACATAGGCGGCTTCCTGGCCCTGGGGCCGGGGGCTGGGGTGTGGGGCAGTCTGGGTCCTCTCATCATCCC CACTTCCCAGGCCCAGCCTTTCCAACCCTGCCTGGGATCTGGGCTTTAATGCAGAGGCCATGTCCTTGTCTGGTCCTGCTTCTGGCTACAGCCACCCTGG AACGGAGAAGGCAGCTGACGGGGATTGCCTTCCTCAGCCGCAGCAGCACCTGGGGCTCCAGCTGCTGGAATCCTACCATCCCAGGAGGCAGGCACAGCCA GGGAGAGGGGAGGAGTGGGCAGTGAAGATGAAGCCCCATGCTCAGTCCCCTCCCATCCCCCACGCAGCTCCACCCCAGTCCCAAGCCACCAGCTGTCTGC TCCTGGTGGGAGGTGGCCTCCTCAGCCCCTCCTCTCTGACCTTTAACCTCACTCTCACCTTGCACCGTGCACCAACCCTTCACCCCTCCTGGAAAGCAGG CCTGATGGCTTCCCACTGGCCTCCACCACCTGACCAGAGTGTTCTCTTCAGAGGACTGGCTCCTTTCCCAGTGTCCTTAAAATAAAGAAATGAAAATGCT TGTTGGCACATTCA

LGALS9.F5 (forward primer)
LGALS9.R1 (reverse primer)
Multiple exon skipping

Frame 0

CLLNYM*GQGLPTFPELVSSSWAEVILLPQPLK VLVL*A*ATTPCPFF*VGLFYTPVCCE*EFQLLR IAINL*LPTNDVP**NCPWCPRGSQRISLPFSP FCSPCTVYSLNWYLIQEHNGDGQCRWHTWPQSI GASQRN*SANSSASRWSTGRKRGADSRMALGHLP SSRTRTALTCQHLPYPA*VLGLLTSTSLGPGRL QVSLFHGSLPHDLHMPYVPSQPHVT*NVLSCPR LLSSS*SAENKDGEEKEKRIKRDSLRGRIRSSWA PHTVE*KYSLWCFSCKIFPST*LCFYMNNINLN RRNTF*RV*ESS*SSGGHELAV*PGACPLDSEP QLLCM*NGLKPPFPTSTLCTGNAQPHYFLDSVAK HA*DTQPHTSGQCHLATCCHVSVVKHCRPL*TK YASGLG*AQEREEGKVTGAGSAYFPL*VSPQST QYHTFLSLDPLPFAAGSSPLNNSQVALVSISLLS RSLYKGLPSGCERQRWPQRRRRDGLQRFPGSLP ESSCPLFWDYSRRSPGRTSDHCQWDRSQLQWNQ VCCELSDWLQWK*HCLPLQPSV*RWRVRGVQHEA ERKLGARGEEDTHAFPEGDAL*PLLPGAELRFQ GDGERDPLRAVLPPRALPPCGHHLRQWLCAAVL HQLPEPPHSPCSACLLHGAVLPACLFPTQAQGAQ TKTSRRVACQPGSHYPDSHPHSAERPWTDVLYS RHPTYDVPPPRLSDAFHHHHSGRAVPIQVHPPV RHCPAQCSEVPHQPVLWEPHRLPPEPPF**ECCG PQHPDRQLLGV*GAKSAPKNALRPWPELLSVDL V*SSLPQGGRGWSAPV*ILPSPEEPAHHQQTGS GGRHPADPCADIGGFLALGPGAGVWGSLGPLIIP TSQAQPFQPCLGSGL*CRGHVLVWSCFWLQPPW NGEGS*RGLPSSAAAAPGAPAAGILPSQEAGTA RERGGVGSEDEAPCSVPSHPPRSSTPVPSHQLSA PGGRWPPQPLLSDL*PHSHLAPCTNPSPLLESR PDGFPLASTT*PECSLQRTGSFPSVLKIKK*KC LLAHS

Frame 1

VC*IICRDRVFQPFPSWSQAPGLK*SSCLSLSK CWYYRHKPPRPAHFSKLDYFTLQSAVNESSSCC VSQSICDCLQMMCRDEIVPGVREDRKEFPFLSLL SAPLVLSTP*TGT*SRNIMGMDSADGTHGLSLL EHLKGISLQILVHLGGAQEGREVQTHVWPWGTC HPLAPVQPLPASTYPTQLEFWAFSPAHRWDLVDF R*VYFMAPSPTTCTCHMFLPSPMSLEMFYHVQD FSPHPKVQKIRMEKRRRRESREIH*EGGSEAPG HPIL*NKNIHYGVFLAKYSPLHNYVFT*II*I*T GEILFEESENLHKVQAATN*LCDLGHAP*TLSL SFSVCKMG*SRPSPQAPCAQAMPSPIIFWTPWP SMLRTHSHILLGSVIWQLAVMSVWSSIVDLYEPN MLQAWVEHRRGRRERSLGLGVPTSLCECHPSPP STIPFSLWTHFLLLPAPPH*ITAKLLWFLFLC* VVPSTKDFLVGVKGSGGHRGGGEMAFSGSQAPYL SPAVPFSGTIQGGLQDGLQITVNGTVLSSSGTR FAVNFQTGFSGNDIAFHFNPRFEDGGYVVCNTR QNGSWGPEERKTHMPFQKGMPFDLCFLVQSSDFK VMVNGILFVQYFHRVPFHRVDTISVNGSVQLSY ISFQNPRTVPVQPAFSTVPFSQPVCFPPRPRGR RQKPPGVWPANPAPITQTVIHTVQSAPGQMFSTP AIPPMMYPHPAYPMPFITTILGGLYPSKSILLS GTVLPSAQRFHINLCSGNHIAFHLNPRFDENAV VRNTQIDNSWGSEERSLPRKMPFVRGQSFSVWIL CEAHCLKVAVDGQHLFEYYHRLRNLPTINRLEV GGDIQLTHVQT*AASWPWGRGLGCGAVWVLSSS PLPRPSLSNPAWDLGFNAEAMSLSGPASGYSHPG TEKAADGDCLPQPQQHLGLQLLESYHPRRQAQP GRGEEWAVKMKPHAQSPPIPHAAPPQSQATSCL LLVGGGLLSPSSLTFNLTLTLHRAPTLHPSWKAG LMASHWPPPPDQSVLFRGLAPFPVSLK*RNENA CWHI

Frame 2

FVKLYVGTGSSNLSRAGLKLLG*SDPPASASQS AGIIGISHHALPIFLSWIILHSSLL*MRVPVVA YRNQFVIAYK*CAVMKLSLVSERIAKNFPSFLS FLLPLYCLLLKLVLDPGT*WGWTVQMAHMASVYW SISKELVCKF*CI*VEHRKEERCRLTYGPGAPA ILSHPYSPYLPALTLPSLSSGPSHQHIAGTWST SGKFISWLPPPRPAHAICSFPAPCHLKCSIMSKT SLLILKCRK*GWRREGEENQERFIEREDQKLLG TPYCRIKIFIMVFFLQNIPLYIIMFLHE*YKFK QEKYFLKSLRIFIKFRRPRISCVTWGMPLRL*AS ASLYVKWVEAALPHKHPVHRQCPAPLFSGLRGQ ACLGHTATYFWAVSSGNLLSCQCGQAL*TSMNQ ICFRLGLSTGEGGGKGHWGWECLLPSVSVTPVHP VPYLSLSGPTSFCCRLLPIE*QPSCFGFYFFVK SFPLQRTS*WV*KAAVATEAAERWPSAVPRLPT *VQLSPFLGLFKEVSRTDFRSLSMGPFSAPVEPG LL*TFRLASVEMTLPSTSTLGLKMEGTWCATRG RTEAGGPRRGRHTCLSRRGCPLTSASWCRAQIS R*W*TGSSSCSTSTACPSTVWTPSPSMALCSCPT SASRTPAQSLFSLPSPRCRSPSLSVSHPGPGGA DKNLPACGLPTRLPLPRQSSTQCRAPLDRCSLL PPSHL*CTPTPPIRCLSSPPFWEGCTHPSPSSCQ ALSCPVLRGSTSTCALGTTSPST*TPVLMRMLW SATPRSTTPGGLRSEVCPEKCPSSVARASQCGS CVKLTASRWPWMVSTCLNTTIA*GTCPPSTDWKW GATSS*PMCRHRRLPGPGAGGWGVGQSGSSHHP HFPGPAFPTLPGIWALMQRPCPCLVLLLATATL ERRRQLTGIAFLSRSSTWGSSCWNPTIPGGRHSQ GEGRSGQ*R*SPMLSPLPSPTQLHPSPKPPAVC SWWEVASSAPPL*PLTSLSPCTVHQPFTPPGKQ A*WLPTGLHHLTRVFSSEDWLLSQCP*NKEMKML VGTF