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Sequences

Sequence HSD17B1.fAug05

AGTTAGGTGGGACTTGGGTTGGGGTAGAGTCAGGTTGTGACCTGCACTCCATTAGCCATGAGACCTCAGGCAAGTTCCTTGCTTTCTCTGAGTGCTTTCC
TTTCCTTTCCCTTTTCTTTTCTTTTTTGTTTTTCTTTTGTTTTGAGACGGAGTTTCACTCTTGTTGCCCAGCCTGGAGTGCAATAGCGCGGTCTCAGTTC
ACCACAACCTCTGCCTCCCGGGTTCAAGTGTAGCTAGGATTGCAGGCATGTGCCACCACGCCCAGCTAATTTTGTATTTTTAGTAGAGACGAGGTTTCTC
CATGTTGGTCAGACTGGTCTTGGACTCCCGACCTCAGGTGATCCGCCCGCCCTGGCCTCTCAGAGTGCTGGGATTACAGGCATGACCCACCCCATCCGGC
CCTCTGAGTCTTTCTTATCTGTAAAATGGGTATAATAATACCTATCTAATCGGTTTTAGTAATGGTGGAGATAATGCCTGAAAGTGCTAGTATAGAGGTT
TAATACCCAGCAAGGACTGTTATTAGAATGAATAGTAATATGACTACTGTCACATTTTGCAAATGTGTAAAGAAGAAAGAGGGCTAAGTGAATCAAAGGG
AGACAGCCCCACTCACCCTGTTCTGCCCCAGAGGACTGAGCGATCCCCACCATCTGGGAAGCTGCCTCACCAGGAGGTCCAGCTGGGGCTACAGGACTGG
CTACTTTGCTACAATGGCCCATCTTTCCTGAGCCCAGTGGAGGGTCCCAGGGGGGCAGAAGTCATTGATAGGGGCCAGTAGGGTTGTAGAGCCACTGTCT
GAACTTCTGTGGAGGTCTGGTGCAGGGGAGGTGTGAACAGATAGGAGGCTAGGTGAGGATGCAGCAGAGGAAGGGGGCAGGAGTGCCCCAGGAGGGGGCG
GTACCGAGGCAGGGATTCAGCTGGGTCTGAGAGGGAGGGAAGGCTGAGGGGGATGGCCCTTTGGAGTGGGCAGGGACATGACCACAGAAAGCCTGGGAAG
TGGAAACAGACAGGAGCCTGCTGGAGCTCTTGGAGCTTTGTGTGGGGCACTAGGGGAAGGAGGCAGGCACTCCACCCTGACCTGCCCCTACCTCTGGATG
AGGGTCTTCTCTGCCTGTTGGATGATGTGCTGCCCCTGCTCTTGGAGGAAGAGGCCTCCCAGCCACCCCCTCCTGCCAGTCGCCTTGCCCTGCTGACCCA
GACAGATCTGCTATCCCTACAGCATCCAGGAAGGCCACAGGGAGGGGGCCCAGGTGGCATGGGTTTCTGAGGCCTGGGATCTGCTCTCAGTCCCGCTCTC
GCCAACCCCCTTTGCCCCTCTGTGACAGTGTGTGTACATATGCGTGCATGTGTGTACCTGTGTGGTGTGGCTCACAAGGTCACCCCTGGGGGTGGGATAA
TAAGAGGTAAAGTGTGACCCCCTCTCTTCCGTACATTCATTTTTTCAAGCTTGTGAATATTCACATTGTTAATATAATCTTCTGCAGTCCCATGACTTCG
AATAGCACTTAGATGCCGATGACCCCCCCACCAAAGAAACCCCACTGTCTCCCACAGACTCCAGGTCTGTATCTCCAACTGCCTACTCAATACAACACGT
CCACCTGATACAATGAACTTAATGTGTCTAAACCCCAATTCCACATTTCCAGTCCTCCTACCGGCTCCTCCCCCAGTCTTCTCTACTTTACTATATGACA
ACTCCATTTTTCCCAATACTGGTTTTAAAAACCCTGGAGTAGTCCTTGACTCCAGTCTCTCTCACCTTTCAACCAATTCAGCAGCAAATACTGACAGCTC
TACCTTTGCTCATCAAAGCATATCCCCAAATCACCCTATTCCAGACTGCACACTGTCATCTCTCCCTTCAATTACAGCAGTAGCCTCCTAACTAGTTTTC
TTGATTCCACTCCTGCCCATCAGCAGTGAAATTACCCCAGAGCAGTTAAAATGATCTTTTAGGATGGGCACAGTGGCTCACGCCTGTAATACCAGCACTT
TGGGAGGTGGAGGCTGGCTGATCCCCTTAGGTTGGGAGTTCAAGACCATCCTCACCAACAGGGAGAAACCCCATCTCTACTAAAAATACAAAAATTAGCC
GGATGTGGTGGGACGTGTCTGTAATCCCAACTACTTGGGAGGCTGAGACAGGAGAACTGCTTGAACCCGGGAGGTGGAGGTTGCAGTGAGCCAGAATCGT
GCCACAGCACTCCAGCCTGGGCAAGAAGCGCGAAACTCCGTCTCAAAATAAAATAATCTTCTAAAAATGACAGGGCCAGGTGGGGTGGCACTTTTTTATA
ATCCGAGCACTTTGGGAGGCTGAGGTGGGCAGATCGCTTGACATCAGGGGTTTGAGACCAGCCTGGCCAACATGGTGAAACTCCGTCTCTACTAAAAATA
CTAAAAATTAGCTGGGCGTGGTGGCGGGTGCCTGTAATCCCAGCTACTCGGGAGGCTGATGCAGGAGAATCGCCTGAACCAGGCAGATGCAGGATGCAGT
GAGCCAAGATTAAGACACTGCACTCCAACCTAAGCAATACTTTGTCTCAAAAATAAAAAAAAGCCTGGGAAACAAAGTGAGACCCCGTCTCTACAAAAAA
GTCAAAAAATTAGCTGGGTATGGTGGCAGTGATGGCACACACCTGTAGTCCCGGCTACTTGGGAAGCTTTTTAATATTTTTTGCAGAGACCGGGTCTCAC
TCTGTTACCTGGCCTGGTCTTGAACTCCTGGGCTCCAACAATCCCCTTTCCTGGGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACCGTGCCCAGC
CTCAAAGCATATTTTAAAGGATAGAAATAAACAGCCATATGAAGAGATACAGACAGGGCGGTCTGGAAGGGTCCAGAGCAGGAGCTTCTATCTCCATAGA
GTTGGGGTTACGTCACCCTCTGGGCACATTCTGTCAGCCTCCACACGTTCAGCCCTCAGAAGCTCCCGAACCCTGTCCTTTGGGCCTTTTATGGAGAACT
CCATTGGCTGTCCATGACTGAAGCATGGACAACTGTGATAATGTGATTGGGCAAAAAGGGTCTGATCTAAGCCCAGCAAGGCCAGTCCAGATTCTTTGGG
CCTTTGTGCAGCATTCCTTTCTCCAGGGTATGGGGCAAGGACCCACTCTGGAATGAGGATCCTACAACCCACAATCAGATTAGAGTCCTGCCTTGGGCAG
CTGAAAAGAGGACAGGAGAAGGTCAGAGAGACGAAAGGCTGTTTTTTGAGGCCTGAGGCACCCCAACATGACAACGTAAGACTGTAACCATGGTCATGTG
AGTTATGAGCTAGGAACCCTGGACGAAACCAGCACATATACAATCATCTCCCACCTCCCAACGCCTTTACTTTCACAGCCTCTGCAGCAAACTGCGGTCA
CTATAATCGCTCCTGTGGCACAGAGGCATACCCAGGGGAATCTGCCCAGGGGGCCACTCTGTGCCCACGTGGGAACCCACACCTGCTTGTAAAGCCTCCC
CTCCCTCTGACCAGCAACCAGGACAGTTTGTTGTTCCAAGCAGTGGGCTCATGTCTGTTTTGGCTCAGAACAGGGTGGGGAGAGCGGGCCAGGGACCCGC
AGGAAGGCTTATCCTTGAGATTGCGTGGGAGACACAACAAGGGGTGGGGGCCCGCAGGCGGGGCGGGGCGAAGCAGGTGATATCAAGCCCAGAGCCCCAG
CCTCTCCCCACAGTCTCACCATGGCCCGCACCGTGGTGCTCATCACCGGCTGTTCCTCGGGCATCGGCCTGCACTTGGCCGTACGTCTGGCTTCAGATCC
ATCCCAGAGCTTCAAAGTGTATGCCACGTTGAGGGACCTGAAAACACAGGGCCGGCTGTGGGAGGCGGCCCGGGCCCTGGCATGCCCTCCGGGATCCCTG
GAGACGTTGCAGCTGGACGTAAGGGACTCAAAATCCGTGGCCGCTGCCCGGGAACGCGTGACTGAGGGCCGCGTGGACGTGCTGGTGTGTAACGCAGGCC
TGGGCCTGCTGGGGCCGCTGGAGGCGCTGGGGGAGGACGCCGTGGCCTCTGTGCTGGACGTGAATGTAGTAGGGACTGTGCGGATGCTGCAGGCCTTCCT
GCCAGACATGAAGAGGCGCGGTTCGGGACGCGTGTTGGTGACCGGGAGCGTGGGAGGATTGATGGCTTGAGCCTGATCGAGTGCGGCCCAGTGCACACCG
CCTTCATGGAGAAGGTGTTGGGCAGCCCAGAGGAGGTGCTGGACCGCACGGACATCCACACCTTCCACCGCTTCTACCAATACCTCGCCCACAGCAAGCA
AGTCTTTCGCGAGGCGGCGCAGAACCCTGAGGAGGTGGCGGAGGTGAGCGCCGGGCTGGACTCCAGGAGTGGGGGCGGTGCGTCCTCCGGCGCGCAGCGG
TGGCCACAGCTCTCCTCCCGCCGCCGCAGGTCTTCCTCACCGCTTTGCGCGCCCCGAAGCCGACCCTGCGCTACTTCACCACCGAGCGCTTCCTGCCCCT
GCTGCGGATGCGCCTGGACGACCCCAGCGGCTCCAACTACGTCACCGCCATGCACCGGGAAGTGTTCGGCGACGTTCCGGCAAAGGCCGAGGCTGGGGCC
GAGGCTGGGGGCGGGGCCGGGCCTGGGGCAGAGGACGAGGCCGGGCGCGGTGCGGTGGGGGACCCTGAGCTCGGCGATCCTCCGGCCGCCCCGCAGTAAA
GGCTTCCTCAGCCGCTGTCTCCCGCGCCCTTCTTTGTCCCCTGGGTCTGTGTGGTCCCTGGGGATGGGGCGGCGGTAGCAGCTGTGGGTGGCTAATTAAG
ATAGATCGCGTTAGCCAGTTTTACCAGCGCAGCTAGGCGCGATGGCTGTCGCCTGTAATGCCAGCGCTTTGGGAGGCGGAGGCAGGAGGATCGCTCAAGC
CCCGGAGTTGGAGACCAGCCAGAGCAACACAGTGAGACCCCCATCTCTACAAAAATAAAGAAAATTT
  • HSD17B1.F4 (forward primer)
  • HSD17B1.R4 (reverse primer)
  • Alternative donor

Frame 0

S*VGLGLG*SQVVTCTPLAMRPQASSLLSLSAF
LSFPFSFLFCFSFVLRRSFTLVAQPGVQ*RGLSS
PQPLPPGFKCS*DCRHVPPRPANFVFLVETRFL
HVGQTGLGLPTSGDPPALASQSAGITGMTHPIR
PSESFLSVKWV**YLSNRF**WWR*CLKVLV*RF
NTQQGLLLE*IVI*LLSHFANV*RRKRAK*IKG
RQPHSPCSAPED*AIPTIWEAASPGGPAGATGL
ATLLQWPIFPEPSGGSQGGRSH**GPVGL*SHCL
NFCGGLVQGRCEQIGG*VRMQQRKGAGVPQEGA
VPRQGFSWV*EGGKAEGDGPLEWAGT*PQKAWE
VETDRSLLELLELCVGH*GKEAGTPP*PAPTSG*
GSSLPVG*CAAPALGGRGLPATPSCQSPCPADP
DRSAIPTASRKATGRGPRWHGFLRPGICSQSRS
RQPPLPLCDSVCTYACMCVPVWCGSQGHPWGWDN
KR*SVTPSLPYIHFFKLVNIHIVNIIFCSPMTS
NST*MPMTPPPKKPHCLPQTPGLYLQLPTQYNT
ST*YNELNVSKPQFHISSPPTGSSPSLLYFTI*Q
LHFSQYWF*KPWSSP*LQSLSPFNQFSSKY*QL
YLCSSKHIPKSPYSRLHTVISPFNYSSSLLTSF
LDSTPAHQQ*NYPRAVKMIF*DGHSGSRL*YQHF
GRWRLADPLRLGVQDHPHQQGETPSLLKIQKLA
GCGGTCL*SQLLGRLRQENCLNPGGGGCSEPES
CHSTPAWARSAKLRLKIK*SSKNDRARWGGTFL*
SEHFGRLRWADRLTSGV*DQPGQHGETPSLLKI
LKISWAWWRVPVIPATREADAGESPEPGRCRMQ
*AKIKTLHSNLSNTLSQK*KKAWETK*DPVSTKK
SKN*LGMVAVMAHTCSPGYLGSFLIFFAETGSH
SVTWPGLELLGSNNPLSWASQSAGITGMSHRAQ
PQSIF*RIEINSHMKRYRQGGLEGSRAGASISIE
LGLRHPLGTFCQPPHVQPSEAPEPCPLGLLWRT
PLAVHD*SMDNCDNVIGQKGSDLSPARPVQILW
AFVQHSFLQGMGQGPTLE*GSYNPQSD*SPALGS
*KEDRRRSERRKAVF*GLRHPNMTT*DCNHGHV
SYELGTLDETSTYTIISHLPTPLLSQPLQQTAV
TIIAPVAQRHTQGNLPRGPLCAHVGTHTCL*SLP
SL*PATRTVCCSKQWAHVCFGSEQGGESGPGTR
RKAYP*DCVGDTTRGGGPQAGRGEAGDIKPRAP
ASPHSLTMARTVVLITGCSSGIGLHLAVRLASDP
SQSFKVYATLRDLKTQGRLWEAARALACPPGSL
ETLQLDVRDSKSVAAARERVTEGRVDVLVCNAG
LGLLGPLEALGEDAVASVLDVNVVGTVRMLQAFL
PDMKRRGSGRVLVTGSVGGLMA*A*SSAAQCTP
PSWRRCWAAQRRCWTARTSTPSTASTNTSPTAS
KSFARRRRTLRRWRR*APGWTPGVGAVRPPARSG
GHSSPPAAAGLPHRFARPEADPALLHHRALPAP
AADAPGRPQRLQLRHRHAPGSVRRRSGKGRGWG
RGWGRGRAWGRGRGRARCGGGP*ARRSSGRPAVK
ASSAAVSRALLCPLGLCGPWGWGGGSSCGWLIK
IDRVSQFYQRS*ARWLSPVMPALWEAEAGGSLK
PRSWRPARATQ*DPHLYKNKEN

Frame 1

VRWDLGWGRVRL*PALH*P*DLRQVPCFL*VLS
FPFPFLFFFVFLLF*DGVSLLLPSLECNSAVSV
HHNLCLPGSSVARIAGMCHHAQLILYF**RRGFS
MLVRLVLDSRPQVIRPPWPLRVLGLQA*PTPSG
PLSLSYL*NGYNNTYLIGFSNGGDNA*KC*YRG
LIPSKDCY*NE**YDYCHILQMCKEERGLSESKG
DSPTHPVLPQRTERSPPSGKLPHQEVQLGLQDW
LLCYNGPSFLSPVEGPRGAEVIDRGQ*GCRATV
*TSVEVWCRGGVNR*EAR*GCSRGRGQECPRRGR
YRGRDSAGSEREGRLRGMALWSGQGHDHRKPGK
WKQTGACWSSWSFVWGTRGRRQALHPDLPLPLD
EGLLCLLDDVLPLLLEEEASQPPPPASRLALLTQ
TDLLSLQHPGRPQGGGPGGMGF*GLGSALSPAL
ANPLCPSVTVCVHMRACVYLCGVAHKVTPGGGI
IRGKV*PPLFRTFIFSSL*IFTLLI*SSAVP*LR
IALRCR*PPHQRNPTVSHRLQVCISNCLLNTTR
PPDTMNLMCLNPNSTFPVLLPAPPPVFSTLLYD
NSIFPNTGFKNPGVVLDSSLSHLSTNSAANTDSS
TFAHQSISPNHPIPDCTLSSLPSITAVAS*LVF
LIPLLPISSEITPEQLK*SFRMGTVAHACNTST
LGGGGWLIPLGWEFKTILTNREKPHLY*KYKN*P
DVVGRVCNPNYLGG*DRRTA*TREVEVAVSQNR
ATALQPGQEARNSVSK*NNLLKMTGPGGVALFY
NPSTLGG*GGQIA*HQGFETSLANMVKLRLY*KY
*KLAGRGGGCL*SQLLGRLMQENRLNQADAGCS
EPRLRHCTPT*AILCLKNKKKPGKQSETPSLQK
SQKISWVWWQ*WHTPVVPATWEAF*YFLQRPGLT
LLPGLVLNSWAPTIPFPGPPKVLGLQA*ATVPS
LKAYFKG*K*TAI*RDTDRAVWKGPEQELLSP*
SWGYVTLWAHSVSLHTFSPQKLPNPVLWAFYGEL
HWLSMTEAWTTVIM*LGKKGLI*AQQGQSRFFG
PLCSIPFSRVWGKDPLWNEDPTTHNQIRVLPWA
AEKRTGEGQRDERLFFEA*GTPT*QRKTVTMVM*
VMS*EPWTKPAHIQSSPTSQRLYFHSLCSKLRS
L*SLLWHRGIPRGICPGGHSVPTWEPTPACKAS
PPSDQQPGQFVVPSSGLMSVLAQNRVGRAGQGPA
GRLILEIAWETQQGVGARRRGGAKQVISSPEPQ
PLPTVSPWPAPWCSSPAVPRASACTWPYVWLQI
HPRASKCMPR*GT*KHRAGCGRRPGPWHALRDPW
RRCSWT*GTQNPWPLPGNA*LRAAWTCWCVTQA
WACWGRWRRWGRTPWPLCWT*M**GLCGCCRPS
CQT*RGAVRDACW*PGAWED*WLEPDRVRPSAHR
LHGEGVGQPRGGAGPHGHPHLPPLLPIPRPQQA
SLSRGGAEP*GGGGGERRAGLQEWGRCVLRRAA
VATALLPPPQVFLTALRAPKPTLRYFTTERFLPL
LRMRLDDPSGSNYVTAMHREVFGDVPAKAEAGA
EAGGGAGPGAEDEAGRGAVGDPELGDPPAAPQ*
RLPQPLSPAPFFVPWVCVVPGDGAAVAAVGG*LR
*IALASFTSAARRDGCRL*CQRFGRRRQEDRSS
PGVGDQPEQHSETPISTKIKKI

Frame 2

LGGTWVGVESGCDLHSISHETSGKFLAFSECFP
FLSLFFSFLFFFCFETEFHSCCPAWSAIARSQF
TTTSASRVQV*LGLQACATTPS*FCIFSRDEVS
PCWSDWSWTPDLR*SARPGLSECWDYRHDPPHPA
L*VFLICKMGIIIPI*SVLVMVEIMPESASIEV
*YPARTVIRMNSNMTTVTFCKCVKKKEG*VNQR
ETAPLTLFCPRGLSDPHHLGSCLTRRSSWGYRTG
YFATMAHLS*AQWRVPGGQKSLIGASRVVEPLS
ELLWRSGAGEV*TDRRLGEDAAEEGGRSAPGGG
GTEAGIQLGLRGREG*GGWPFGVGRDMTTESLGS
GNRQEPAGALGALCGALGEGGRHSTLTCPYLWM
RVFSACWMMCCPCSWRKRPPSHPLLPVALPC*P
RQICYPYSIQEGHREGAQVAWVSEAWDLLSVPLS
PTPFAPL*QCVYICVHVCTCVVWLTRSPLGVG*
*EVKCDPLSSVHSFFQACEYSHC*YNLLQSHDF
E*HLDADDPPTKETPLSPTDSRSVSPTAYSIQHV
HLIQ*T*CV*TPIPHFQSSYRLLPQSSLLYYMT
TPFFPILVLKTLE*SLTPVSLTFQPIQQQILTA
LPLLIKAYPQITLFQTAHCHLSLQLQQ*PPN*FS
*FHSCPSAVKLPQSS*NDLLGWAQWLTPVIPAL
WEVEAG*SP*VGSSRPSSPTGRNPISTKNTKIS
RMWWDVSVIPTTWEAETGELLEPGRWRLQ*ARIV
PQHSSLGKKRETPSQNKIIF*K*QGQVGWHFFI
IRALWEAEVGRSLDIRGLRPAWPTW*NSVSTKN
TKN*LGVVAGACNPSYSGG*CRRIA*TRQMQDAV
SQD*DTALQPKQYFVSKIKKSLGNKVRPRLYKK
VKKLAGYGGSDGTHL*SRLLGKLFNIFCRDRVS
LCYLAWS*TPGLQQSPFLGLPKCWDYRHEPPCPA
SKHILKDRNKQPYEEIQTGRSGRVQSRSFYLHR
VGVTSPSGHILSASTRSALRSSRTLSFGPFMEN
SIGCP*LKHGQL**CDWAKRV*SKPSKASPDSLG
LCAAFLSPGYGARTHSGMRILQPTIRLESCLGQ
LKRGQEKVRETKGCFLRPEAPQHDNVRL*PWSC
EL*ARNPGRNQHIYNHLPPPNAFTFTASAANCGH
YNRSCGTEAYPGESAQGATLCPRGNPHLLVKPP
LPLTSNQDSLLFQAVGSCLFWLRTGWGERARDP
QEGLSLRLRGRHNKGWGPAGGAGRSR*YQAQSPS
LSPQSHHGPHRGAHHRLFLGHRPALGRTSGFRS
IPELQSVCHVEGPENTGPAVGGGPGPGMPSGIP
GDVAAGRKGLKIRGRCPGTRD*GPRGRAGV*RRP
GPAGAAGGAGGGRRGLCAGRECSRDCADAAGLP
ARHEEARFGTRVGDRERGRIDGLSLIECGPVHT
AFMEKVLGSPEEVLDRTDIHTFHRFYQYLAHSKQ
VFREAAQNPEEVAEVSAGLDSRSGGGASSGAQR
WPQLSSRRRRSSSPLCAPRSRPCATSPPSASCP
CCGCAWTTPAAPTTSPPCTGKCSATFRQRPRLGP
RLGAGPGLGQRTRPGAVRWGTLSSAILRPPRSK
GFLSRCLPRPSLSPGSVWSLGMGRR*QLWVAN*
DRSR*PVLPAQLGAMAVACNASALGGGGRRIAQA
PELETSQSNTVRPPSLQK*RKF

Sequence HSD17B1.dAug05

AGGACCCACTCTGGAATGAGGATCCTACAACCCACAATCAGATTAGAGTCCTGCCTTGGGCAGCTGAAAAGAGGACAGGAGAAGGTCAGAGAGACGAAAG
GCTGTTTTTTGAGGCCTGAGGCACCCCAACATGACAACGTAAGACTGTAACCATGGTCATGTGAGTTATGAGCTAGGAACCCTGGACGAAACCAGCACAT
ATACAATCATCTCCCACCTCCCAACGCCTTTACTTTCACAGCCTCTGCAGCAAACTGCGGTCACTATAATCGCTCCTGTGGCACAGAGGCATACCCAGGG
GAATCTGCCCAGGGGGCCACTCTGTGCCCACGTGGGAACCCACACCTGCTTGTAAAGCCTCCCCTCCCTCTGACCAGCAACCAGGACAGTTTGTTGTTCC
AAGCAGTGGGCTCATGTCTGTTTTGGCTCAGAACAGGGTGGGGAGAGCGGGCCAGGGACCCGCAGGAAGGCTTATCCTTGAGATTGCGTGGGAGACACAA
CAAGGGGTGGGGGCCCGCAGGCGGGGCGGGGCGAAGCAGGTGATATCAAGCCCAGAGCCCCAGCCTCTCCCCACAGTCTCACCATGGCCCGCACCGTGGT
GCTCATCACCGGCTGTTCCTCGGGCATCGGCCTGCACTTGGCCGTACGTCTGGCTTCAGATCCATCCCAGAGCTTCAAAGGTATAGATAGGCAGGGACAG
GGAGGGAGAGAAGGGAGGAGCCCTTGGAGGCCAGAAGGGAAGTCAGATCTTCCTCCTCTCCCAAAACCTCCAGTGTATGCCACGTTGAGGGACCTGAAAA
CACAGGGCCGGCTGTGGGAGGCGGCCCGGGCCCTGGCATGCCCTCCGGGATCCCTGGAGACGTTGCAGCTGGACGTAAGGGACTCAAAATCCGTGGCCGC
TGCCCGGGAACGCGTGACTGAGGGCCGCGTGGACGTGCTGGTGTGTAACGCAGGCCTGGGCCTGCTGGGGCCGCTGGAGGCGCTGGGGGAGGACGCCGTG
GCCTCTGTGCTGGACGTGAATGTAGTAGGGACTGTGCGGATGCTGCAGGCCTTCCTGCCAGACATGAAGAGGCGCGGTTCGGGACGCGTGTTGGTGACCG
GGAGCGTGGGAGGATTGATGGGTGAGTGGTAGGGAGTGGCCTCGGCAGCTCCAGATTCTTTGTGTGCGGAGCTGAGCCTTGAAGGCAGGTTCCGCGGGGG
GGGTGGAGTGGGGTGCCGTCAGCTTGGAGGGGCACCGTCTGCCCGGGGATGACCCCCTGGCCGCTGCGCCTCAGGAACCTCGTCTCCCCACCTAAGGGCT
GCCTTTCAATGACGTTTATTGCGCCAGCAAGTTCGCGCTCGAAGGCTTATGCGAGAGTCTGGCGGTTCTGCTGCTGCCCTTTGGGGTCCACTTGAGCCTG
ATCGAGTGCGGCCCAGTGCACACCGCCTTCATGGAGAAGGTGTTGGGCAGCCCAGAGGAGGTGCTGGACCGCACGGACATCCACACCTTCCACCGCTTCT
ACCAATACCTCGCCCACAGCAAGCAAGTCTTTCGCGAGGCGGCGCAGAACCCTGAGGAGGTGGCGGAGGTCTTCCTCACCGCTTTGCGCGCCCCGAAGCC
GACCCTGCGCTACTTCACCACCGAGCGCTTCCTGCCCCTGCTGCGGATGCGCCTGGACGACCCCAGCGGCTCCAACTACGTCACCGCCATGCACCGGGAA
GTGTTCGGCGACGTTCCGGCAAAGGCCGAGGCTGGGGCCGAGGCTGGGGGCGGGGCCGGGCCTGGGGCAGAGGACGAGGCCGGGCGCGGTGCGGTGGGGG
ACCCTGAGCTCGGCGATCCTCCGGCCGCCCCGCAGTAAAGGCTTCCTCAGCCGCTGTCTCCCGCGCCCTTCTTTGTCCCCTGGGTCTGTGTGGTCCCTGG
GGATGGGGCGGCGGTAGCAGCTGTGGGTGGCTAATTAAGATAGATCGCGTTAGCCAGTTTTACCAGCGCAGCTAGGCGCGATGGCTGTCGCCTGTAATGC
CAGCGCTTTGGGAGGCGGAGGCAGGAGGATCGCTCAAGCCCCGGAGTTGGAGACCAGCCAGAGCAACACAGTGAGACCCCCATCTCTACAAAAATAAAGA
AAATTTAAAAATCAG
  • HSD17B1.F4 (forward primer)
  • HSD17B1.R4 (reverse primer)
  • Alternative donor

Frame 0

RTHSGMRILQPTIRLESCLGQLKRGQEKVRETK
GCFLRPEAPQHDNVRL*PWSCEL*ARNPGRNQHI
YNHLPPPNAFTFTASAANCGHYNRSCGTEAYPG
ESAQGATLCPRGNPHLLVKPPLPLTSNQDSLLF
QAVGSCLFWLRTGWGERARDPQEGLSLRLRGRHN
KGWGPAGGAGRSR*YQAQSPSLSPQSHHGPHRG
AHHRLFLGHRPALGRTSGFRSIPELQRYR*AGT
GRERREEPLEARREVRSSSSPKTSSVCHVEGPEN
TGPAVGGGPGPGMPSGIPGDVAAGRKGLKIRGR
CPGTRD*GPRGRAGV*RRPGPAGAAGGAGGGRR
GLCAGRECSRDCADAAGLPARHEEARFGTRVGDR
ERGRIDG*VVGSGLGSSRFFVCGAEP*RQVPRG
GWSGVPSAWRGTVCPGMTPWPLRLRNLVSPPKG
CLSMTFIAPASSRSKAYARVWRFCCCPLGST*A*
SSAAQCTPPSWRRCWAAQRRCWTARTSTPSTAS
TNTSPTASKSFARRRRTLRRWRRSSSPLCAPRS
RPCATSPPSASCPCCGCAWTTPAAPTTSPPCTGK
CSATFRQRPRLGPRLGAGPGLGQRTRPGAVRWG
TLSSAILRPPRSKGFLSRCLPRPSLSPGSVWSL
GMGRR*QLWVAN*DRSR*PVLPAQLGAMAVACNA
SALGGGGRRIAQAPELETSQSNTVRPPSLQK*R
KFKNQ

Frame 1

GPTLE*GSYNPQSD*SPALGS*KEDRRRSERRK
AVF*GLRHPNMTT*DCNHGHVSYELGTLDETST
YTIISHLPTPLLSQPLQQTAVTIIAPVAQRHTQG
NLPRGPLCAHVGTHTCL*SLPSL*PATRTVCCS
KQWAHVCFGSEQGGESGPGTRRKAYP*DCVGDT
TRGGGPQAGRGEAGDIKPRAPASPHSLTMARTVV
LITGCSSGIGLHLAVRLASDPSQSFKGIDRQGQ
GGREGRSPWRPEGKSDLPPLPKPPVYATLRDLK
TQGRLWEAARALACPPGSLETLQLDVRDSKSVAA
ARERVTEGRVDVLVCNAGLGLLGPLEALGEDAV
ASVLDVNVVGTVRMLQAFLPDMKRRGSGRVLVT
GSVGGLMGEW*GVASAAPDSLCAELSLEGRFRGG
GGVGCRQLGGAPSARG*PPGRCASGTSSPHLRA
AFQ*RLLRQQVRARRLMRESGGSAAALWGPLEP
DRVRPSAHRLHGEGVGQPRGGAGPHGHPHLPPLL
PIPRPQQASLSRGGAEP*GGGGGLPHRFARPEA
DPALLHHRALPAPAADAPGRPQRLQLRHRHAPG
SVRRRSGKGRGWGRGWGRGRAWGRGRGRARCGGG
P*ARRSSGRPAVKASSAAVSRALLCPLGLCGPW
GWGGGSSCGWLIKIDRVSQFYQRS*ARWLSPVM
PALWEAEAGGSLKPRSWRPARATQ*DPHLYKNKE
NLKI

Frame 2

DPLWNEDPTTHNQIRVLPWAAEKRTGEGQRDER
LFFEA*GTPT*QRKTVTMVM*VMS*EPWTKPAH
IQSSPTSQRLYFHSLCSKLRSL*SLLWHRGIPR
GICPGGHSVPTWEPTPACKASPPSDQQPGQFVVP
SSGLMSVLAQNRVGRAGQGPAGRLILEIAWETQ
QGVGARRRGGAKQVISSPEPQPLPTVSPWPAPW
CSSPAVPRASACTWPYVWLQIHPRASKV*IGRDR
EGEKGGALGGQKGSQIFLLSQNLQCMPR*GT*K
HRAGCGRRPGPWHALRDPWRRCSWT*GTQNPWP
LPGNA*LRAAWTCWCVTQAWACWGRWRRWGRTPW
PLCWT*M**GLCGCCRPSCQT*RGAVRDACW*P
GAWED*WVSGREWPRQLQILCVRS*ALKAGSAG
GVEWGAVSLEGHRLPGDDPLAAAPQEPRLPT*GL
PFNDVYCASKFALEGLCESLAVLLLPFGVHLSL
IECGPVHTAFMEKVLGSPEEVLDRTDIHTFHRF
YQYLAHSKQVFREAAQNPEEVAEVFLTALRAPKP
TLRYFTTERFLPLLRMRLDDPSGSNYVTAMHRE
VFGDVPAKAEAGAEAGGGAGPGAEDEAGRGAVG
DPELGDPPAAPQ*RLPQPLSPAPFFVPWVCVVPG
DGAAVAAVGG*LR*IALASFTSAARRDGCRL*C
QRFGRRRQEDRSSPGVGDQPEQHSETPISTKIK
KI*KS

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