Sequence HSD17B1.bAug05
AGTTAGGTGGGACTTGGGTTGGGGTAGAGTCAGGTTGTGACCTGCACTCCATTAGCCATGAGACCTCAGGCAAGTTCCTTGCTTTCTCTGAGTGCTTTCC
TTTCCTTTCCCTTTTCTTTTCTTTTTTGTTTTTCTTTTGTTTTGAGACGGAGTTTCACTCTTGTTGCCCAGCCTGGAGTGCAATAGCGCGGTCTCAGTTC
ACCACAACCTCTGCCTCCCGGGTTCAAGTGTAGCTAGGATTGCAGGCATGTGCCACCACGCCCAGCTAATTTTGTATTTTTAGTAGAGACGAGGTTTCTC
CATGTTGGTCAGACTGGTCTTGGACTCCCGACCTCAGGTGATCCGCCCGCCCTGGCCTCTCAGAGTGCTGGGATTACAGGCATGACCCACCCCATCCGGC
CCTCTGAGTCTTTCTTATCTGTAAAATGGGTATAATAATACCTATCTAATCGGTTTTAGTAATGGTGGAGATAATGCCTGAAAGTGCTAGTATAGAGGTT
TAATACCCAGCAAGGACTGTTATTAGAATGAATAGTAATATGACTACTGTCACATTTTGCAAATGTGTAAAGAAGAAAGAGGGCTAAGTGAATCAAAGGG
AGACAGCCCCACTCACCCTGTTCTGCCCCAGAGGACTGAGCGATCCCCACCATCTGGGAAGCTGCCTCACCAGGAGGTCCAGCTGGGGCTACAGGACTGG
CTACTTTGCTACAATGGCCCATCTTTCCTGAGCCCAGTGGAGGGTCCCAGGGGGGCAGAAGTCATTGATAGGGGCCAGTAGGGTTGTAGAGCCACTGTCT
GAACTTCTGTGGAGGTCTGGTGCAGGGGAGGTGTGAACAGATAGGAGGCTAGGTGAGGATGCAGCAGAGGAAGGGGGCAGGAGTGCCCCAGGAGGGGGCG
GTACCGAGGCAGGGATTCAGCTGGGTCTGAGAGGGAGGGAAGGCTGAGGGGGATGGCCCTTTGGAGTGGGCAGGGACATGACCACAGAAAGCCTGGGAAG
TGGAAACAGACAGGAGCCTGCTGGAGCTCTTGGAGCTTTGTGTGGGGCACTAGGGGAAGGAGGCAGGCACTCCACCCTGACCTGCCCCTACCTCTGGATG
AGGGTCTTCTCTGCCTGTTGGATGATGTGCTGCCCCTGCTCTTGGAGGAAGAGGCCTCCCAGCCACCCCCTCCTGCCAGTCGCCTTGCCCTGCTGACCCA
GACAGATCTGCTATCCCTACAGCATCCAGGAAGGCCACAGGGAGGGGGCCCAGGTGGCATGGGTTTCTGAGGCCTGGGATCTGCTCTCAGTCCCGCTCTC
GCCAACCCCCTTTGCCCCTCTGTGACAGTGTGTGTACATATGCGTGCATGTGTGTACCTGTGTGGTGTGGCTCACAAGGTCACCCCTGGGGGTGGGATAA
TAAGAGGTAAAGTGTGACCCCCTCTCTTCCGTACATTCATTTTTTCAAGCTTGTGAATATTCACATTGTTAATATAATCTTCTGCAGTCCCATGACTTCG
AATAGCACTTAGATGCCGATGACCCCCCCACCAAAGAAACCCCACTGTCTCCCACAGACTCCAGGTCTGTATCTCCAACTGCCTACTCAATACAACACGT
CCACCTGATACAATGAACTTAATGTGTCTAAACCCCAATTCCACATTTCCAGTCCTCCTACCGGCTCCTCCCCCAGTCTTCTCTACTTTACTATATGACA
ACTCCATTTTTCCCAATACTGGTTTTAAAAACCCTGGAGTAGTCCTTGACTCCAGTCTCTCTCACCTTTCAACCAATTCAGCAGCAAATACTGACAGCTC
TACCTTTGCTCATCAAAGCATATCCCCAAATCACCCTATTCCAGACTGCACACTGTCATCTCTCCCTTCAATTACAGCAGTAGCCTCCTAACTAGTTTTC
TTGATTCCACTCCTGCCCATCAGCAGTGAAATTACCCCAGAGCAGTTAAAATGATCTTTTAGGATGGGCACAGTGGCTCACGCCTGTAATACCAGCACTT
TGGGAGGTGGAGGCTGGCTGATCCCCTTAGGTTGGGAGTTCAAGACCATCCTCACCAACAGGGAGAAACCCCATCTCTACTAAAAATACAAAAATTAGCC
GGATGTGGTGGGACGTGTCTGTAATCCCAACTACTTGGGAGGCTGAGACAGGAGAACTGCTTGAACCCGGGAGGTGGAGGTTGCAGTGAGCCAGAATCGT
GCCACAGCACTCCAGCCTGGGCAAGAAGCGCGAAACTCCGTCTCAAAATAAAATAATCTTCTAAAAATGACAGGGCCAGGTGGGGTGGCACTTTTTTATA
ATCCGAGCACTTTGGGAGGCTGAGGTGGGCAGATCGCTTGACATCAGGGGTTTGAGACCAGCCTGGCCAACATGGTGAAACTCCGTCTCTACTAAAAATA
CTAAAAATTAGCTGGGCGTGGTGGCGGGTGCCTGTAATCCCAGCTACTCGGGAGGCTGATGCAGGAGAATCGCCTGAACCAGGCAGATGCAGGATGCAGT
GAGCCAAGATTAAGACACTGCACTCCAACCTAAGCAATACTTTGTCTCAAAAATAAAAAAAAGCCTGGGAAACAAAGTGAGACCCCGTCTCTACAAAAAA
GTCAAAAAATTAGCTGGGTATGGTGGCAGTGATGGCACACACCTGTAGTCCCGGCTACTTGGGAAGCTTTTTAATATTTTTTGCAGAGACCGGGTCTCAC
TCTGTTACCTGGCCTGGTCTTGAACTCCTGGGCTCCAACAATCCCCTTTCCTGGGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACCGTGCCCAGC
CTCAAAGCATATTTTAAAGGATAGAAATAAACAGCCATATGAAGAGATACAGACAGGGCGGTCTGGAAGGGTCCAGAGCAGGAGCTTCTATCTCCATAGA
GTTGGGGTTACGTCACCCTCTGGGCACATTCTGTCAGCCTCCACACGTTCAGCCCTCAGAAGCTCCCGAACCCTGTCCTTTGGGCCTTTTATGGAGAACT
CCATTGGCTGTCCATGACTGAAGCATGGACAACTGTGATAATGTGATTGGGCAAAAAGGGTCTGATCTAAGCCCAGCAAGGCCAGTCCAGATTCTTTGGG
CCTTTGTGCAGCATTCCTTTCTCCAGGGTATGGGGCAAGGACCCACTCTGGAATGAGGATCCTACAACCCACAATCAGATTAGAGTCCTGCCTTGGGCAG
CTGAAAAGAGGACAGGAGAAGGTCAGAGAGACGAAAGGCTGTTTTTTGAGGCCTGAGGCACCCCAACATGACAACGTAAGACTGTAACCATGGTCATGTG
AGTTATGAGCTAGGAACCCTGGACGAAACCAGCACATATACAATCATCTCCCACCTCCCAACGCCTTTACTTTCACAGCCTCTGCAGCAAACTGCGGTCA
CTATAATCGCTCCTGTGGCACAGAGGCATACCCAGGGGAATCTGCCCAGGGGGCCACTCTGTGCCCACGTGGGAACCCACACCTGCTTGTAAAGCCTCCC
CTCCCTCTGACCAGCAACCAGGACAGTTTGTTGTTCCAAGCAGTGGGCTCATGTCTGTTTTGGCTCAGAACAGGGTGGGGAGAGCGGGCCAGGGACCCGC
AGGAAGGCTTATCCTTGAGATTGCGTGGGAGACACAACAAGGGGTGGGGGCCCGCAGGCGGGGCGGGGCGAAGCAGGTGATATCAAGCCCAGAGCCCCAG
CCTCTCCCCACAGTCTCACCATGGCCCGCACCGTGGTGCTCATCACCGGCTGTTCCTCGGGCATCGGCCTGCACTTGGCCGTACGTCTGGCTTCAGATCC
ATCCCAGAGCTTCAAAGTGTATGCCACGTTGAGGGACCTGAAAACACAGGGCCGGCTGTGGGAGGCGGCCCGGGCCCTGGCATGCCCTCCGGGATCCCTG
GAGACGTTGCAGCTGGACGTAAGGGACTCAAAATCCGTGGCCGCTGCCCGGGAACGCGTGACTGAGGGCCGCGTGGACGTGCTGGTGTGTAACGCAGGCC
TGGGCCTGCTGGGGCCGCTGGAGGCGCTGGGGGAGGACGCCGTGGCCTCTGTGCTGGACGTGAATGTAGTAGGGACTGTGCGGATGCTGCAGGCCTTCCT
GCCAGACATGAAGAGGCGCGGTTCGGGACGCGTGTTGGTGACCGGGAGCGTGGGAGGATTGATGGGGCTGCCTTTCAATGACGTTTATTGCGCCAGCAAG
TTCGCGCTCGAAGGCTTATGCGAGAGTCTGGCGGTTCTGCTGCTGCCCTTTGGGGTCCACAGCTTGAGCCTGATCGAGTGCGGCCCAGTGCACACCGCCT
TCATGGAGAAGGTGTTGGGCAGCCCAGAGGAGGTGCTGGACCGCACGGACATCCACACCTTCCACCGCTTCTACCAATACCTCGCCCACAGCAAGCAAGT
CTTTCGCGAGGCGGCGCAGAACCCTGAGGAGGTGGCGGAGGTCTTCCTCACCGCTTTGCGCGCCCCGAAGCCGACCCTGCGCTACTTCACCACCGAGCGC
TTCCTGCCCCTGCTGCGGATGCGCCTGGACGACCCCAGCGGCTCCAACTACGTCACCGCCATGCACCGGGAAGTGTTCGGCGACGTTCCGGCAAAGGCCG
AGGCTGGGGCCGAGGCTGGGGGCGGGGCCGGGCCTGGGGCAGAGGACGAGGCCGGGCGCGGTGCGGTGGGGGACCCTGAGCTCGGCGATCCTCCGGCCGC
CCCGCAGTAAAGGCTTCCTCAGCCGCTGTCTCCCGCGCCCTTCTTTGTCCCCTGGGTCTGTGTGGTCCCTGGGGATGGGGCGGCGGTAGCAGCTGTGGGT
GGCTAATTAAGATAGATCGCGTTAGCCAGTTTTACCAGCGCAGCTAGGCGCGATGGCTGTCGCCTGTAATGCCAGCGCTTTGGGAGGCGGAGGCAGGAGG
ATCGCTCAAGCCCCGGAGTTGGAGACCAGCCAGAGCAACACAGTGAGACCCCCATCTCTACAAAAATAAAGAAAATTTAAAAATCAG
- HSD17B1.F4 (forward primer)
- HSD17B1.R4 (reverse primer)
- Intron retention
Frame 0
S*VGLGLG*SQVVTCTPLAMRPQASSLLSLSAF
LSFPFSFLFCFSFVLRRSFTLVAQPGVQ*RGLSS
PQPLPPGFKCS*DCRHVPPRPANFVFLVETRFL
HVGQTGLGLPTSGDPPALASQSAGITGMTHPIR
PSESFLSVKWV**YLSNRF**WWR*CLKVLV*RF
NTQQGLLLE*IVI*LLSHFANV*RRKRAK*IKG
RQPHSPCSAPED*AIPTIWEAASPGGPAGATGL
ATLLQWPIFPEPSGGSQGGRSH**GPVGL*SHCL
NFCGGLVQGRCEQIGG*VRMQQRKGAGVPQEGA
VPRQGFSWV*EGGKAEGDGPLEWAGT*PQKAWE
VETDRSLLELLELCVGH*GKEAGTPP*PAPTSG*
GSSLPVG*CAAPALGGRGLPATPSCQSPCPADP
DRSAIPTASRKATGRGPRWHGFLRPGICSQSRS
RQPPLPLCDSVCTYACMCVPVWCGSQGHPWGWDN
KR*SVTPSLPYIHFFKLVNIHIVNIIFCSPMTS
NST*MPMTPPPKKPHCLPQTPGLYLQLPTQYNT
ST*YNELNVSKPQFHISSPPTGSSPSLLYFTI*Q
LHFSQYWF*KPWSSP*LQSLSPFNQFSSKY*QL
YLCSSKHIPKSPYSRLHTVISPFNYSSSLLTSF
LDSTPAHQQ*NYPRAVKMIF*DGHSGSRL*YQHF
GRWRLADPLRLGVQDHPHQQGETPSLLKIQKLA
GCGGTCL*SQLLGRLRQENCLNPGGGGCSEPES
CHSTPAWARSAKLRLKIK*SSKNDRARWGGTFL*
SEHFGRLRWADRLTSGV*DQPGQHGETPSLLKI
LKISWAWWRVPVIPATREADAGESPEPGRCRMQ
*AKIKTLHSNLSNTLSQK*KKAWETK*DPVSTKK
SKN*LGMVAVMAHTCSPGYLGSFLIFFAETGSH
SVTWPGLELLGSNNPLSWASQSAGITGMSHRAQ
PQSIF*RIEINSHMKRYRQGGLEGSRAGASISIE
LGLRHPLGTFCQPPHVQPSEAPEPCPLGLLWRT
PLAVHD*SMDNCDNVIGQKGSDLSPARPVQILW
AFVQHSFLQGMGQGPTLE*GSYNPQSD*SPALGS
*KEDRRRSERRKAVF*GLRHPNMTT*DCNHGHV
SYELGTLDETSTYTIISHLPTPLLSQPLQQTAV
TIIAPVAQRHTQGNLPRGPLCAHVGTHTCL*SLP
SL*PATRTVCCSKQWAHVCFGSEQGGESGPGTR
RKAYP*DCVGDTTRGGGPQAGRGEAGDIKPRAP
ASPHSLTMARTVVLITGCSSGIGLHLAVRLASDP
SQSFKVYATLRDLKTQGRLWEAARALACPPGSL
ETLQLDVRDSKSVAAARERVTEGRVDVLVCNAG
LGLLGPLEALGEDAVASVLDVNVVGTVRMLQAFL
PDMKRRGSGRVLVTGSVGGLMGLPFNDVYCASK
FALEGLCESLAVLLLPFGVHSLSLIECGPVHTA
FMEKVLGSPEEVLDRTDIHTFHRFYQYLAHSKQV
FREAAQNPEEVAEVFLTALRAPKPTLRYFTTER
FLPLLRMRLDDPSGSNYVTAMHREVFGDVPAKA
EAGAEAGGGAGPGAEDEAGRGAVGDPELGDPPAA
PQ*RLPQPLSPAPFFVPWVCVVPGDGAAVAAVG
G*LR*IALASFTSAARRDGCRL*CQRFGRRRQE
DRSSPGVGDQPEQHSETPISTKIKKI*KS
Frame 1
VRWDLGWGRVRL*PALH*P*DLRQVPCFL*VLS
FPFPFLFFFVFLLF*DGVSLLLPSLECNSAVSV
HHNLCLPGSSVARIAGMCHHAQLILYF**RRGFS
MLVRLVLDSRPQVIRPPWPLRVLGLQA*PTPSG
PLSLSYL*NGYNNTYLIGFSNGGDNA*KC*YRG
LIPSKDCY*NE**YDYCHILQMCKEERGLSESKG
DSPTHPVLPQRTERSPPSGKLPHQEVQLGLQDW
LLCYNGPSFLSPVEGPRGAEVIDRGQ*GCRATV
*TSVEVWCRGGVNR*EAR*GCSRGRGQECPRRGR
YRGRDSAGSEREGRLRGMALWSGQGHDHRKPGK
WKQTGACWSSWSFVWGTRGRRQALHPDLPLPLD
EGLLCLLDDVLPLLLEEEASQPPPPASRLALLTQ
TDLLSLQHPGRPQGGGPGGMGF*GLGSALSPAL
ANPLCPSVTVCVHMRACVYLCGVAHKVTPGGGI
IRGKV*PPLFRTFIFSSL*IFTLLI*SSAVP*LR
IALRCR*PPHQRNPTVSHRLQVCISNCLLNTTR
PPDTMNLMCLNPNSTFPVLLPAPPPVFSTLLYD
NSIFPNTGFKNPGVVLDSSLSHLSTNSAANTDSS
TFAHQSISPNHPIPDCTLSSLPSITAVAS*LVF
LIPLLPISSEITPEQLK*SFRMGTVAHACNTST
LGGGGWLIPLGWEFKTILTNREKPHLY*KYKN*P
DVVGRVCNPNYLGG*DRRTA*TREVEVAVSQNR
ATALQPGQEARNSVSK*NNLLKMTGPGGVALFY
NPSTLGG*GGQIA*HQGFETSLANMVKLRLY*KY
*KLAGRGGGCL*SQLLGRLMQENRLNQADAGCS
EPRLRHCTPT*AILCLKNKKKPGKQSETPSLQK
SQKISWVWWQ*WHTPVVPATWEAF*YFLQRPGLT
LLPGLVLNSWAPTIPFPGPPKVLGLQA*ATVPS
LKAYFKG*K*TAI*RDTDRAVWKGPEQELLSP*
SWGYVTLWAHSVSLHTFSPQKLPNPVLWAFYGEL
HWLSMTEAWTTVIM*LGKKGLI*AQQGQSRFFG
PLCSIPFSRVWGKDPLWNEDPTTHNQIRVLPWA
AEKRTGEGQRDERLFFEA*GTPT*QRKTVTMVM*
VMS*EPWTKPAHIQSSPTSQRLYFHSLCSKLRS
L*SLLWHRGIPRGICPGGHSVPTWEPTPACKAS
PPSDQQPGQFVVPSSGLMSVLAQNRVGRAGQGPA
GRLILEIAWETQQGVGARRRGGAKQVISSPEPQ
PLPTVSPWPAPWCSSPAVPRASACTWPYVWLQI
HPRASKCMPR*GT*KHRAGCGRRPGPWHALRDPW
RRCSWT*GTQNPWPLPGNA*LRAAWTCWCVTQA
WACWGRWRRWGRTPWPLCWT*M**GLCGCCRPS
CQT*RGAVRDACW*PGAWED*WGCLSMTFIAPAS
SRSKAYARVWRFCCCPLGSTA*A*SSAAQCTPP
SWRRCWAAQRRCWTARTSTPSTASTNTSPTASK
SFARRRRTLRRWRRSSSPLCAPRSRPCATSPPSA
SCPCCGCAWTTPAAPTTSPPCTGKCSATFRQRP
RLGPRLGAGPGLGQRTRPGAVRWGTLSSAILRP
PRSKGFLSRCLPRPSLSPGSVWSLGMGRR*QLWV
AN*DRSR*PVLPAQLGAMAVACNASALGGGGRR
IAQAPELETSQSNTVRPPSLQK*RKFKNQ
Frame 2
LGGTWVGVESGCDLHSISHETSGKFLAFSECFP
FLSLFFSFLFFFCFETEFHSCCPAWSAIARSQF
TTTSASRVQV*LGLQACATTPS*FCIFSRDEVS
PCWSDWSWTPDLR*SARPGLSECWDYRHDPPHPA
L*VFLICKMGIIIPI*SVLVMVEIMPESASIEV
*YPARTVIRMNSNMTTVTFCKCVKKKEG*VNQR
ETAPLTLFCPRGLSDPHHLGSCLTRRSSWGYRTG
YFATMAHLS*AQWRVPGGQKSLIGASRVVEPLS
ELLWRSGAGEV*TDRRLGEDAAEEGGRSAPGGG
GTEAGIQLGLRGREG*GGWPFGVGRDMTTESLGS
GNRQEPAGALGALCGALGEGGRHSTLTCPYLWM
RVFSACWMMCCPCSWRKRPPSHPLLPVALPC*P
RQICYPYSIQEGHREGAQVAWVSEAWDLLSVPLS
PTPFAPL*QCVYICVHVCTCVVWLTRSPLGVG*
*EVKCDPLSSVHSFFQACEYSHC*YNLLQSHDF
E*HLDADDPPTKETPLSPTDSRSVSPTAYSIQHV
HLIQ*T*CV*TPIPHFQSSYRLLPQSSLLYYMT
TPFFPILVLKTLE*SLTPVSLTFQPIQQQILTA
LPLLIKAYPQITLFQTAHCHLSLQLQQ*PPN*FS
*FHSCPSAVKLPQSS*NDLLGWAQWLTPVIPAL
WEVEAG*SP*VGSSRPSSPTGRNPISTKNTKIS
RMWWDVSVIPTTWEAETGELLEPGRWRLQ*ARIV
PQHSSLGKKRETPSQNKIIF*K*QGQVGWHFFI
IRALWEAEVGRSLDIRGLRPAWPTW*NSVSTKN
TKN*LGVVAGACNPSYSGG*CRRIA*TRQMQDAV
SQD*DTALQPKQYFVSKIKKSLGNKVRPRLYKK
VKKLAGYGGSDGTHL*SRLLGKLFNIFCRDRVS
LCYLAWS*TPGLQQSPFLGLPKCWDYRHEPPCPA
SKHILKDRNKQPYEEIQTGRSGRVQSRSFYLHR
VGVTSPSGHILSASTRSALRSSRTLSFGPFMEN
SIGCP*LKHGQL**CDWAKRV*SKPSKASPDSLG
LCAAFLSPGYGARTHSGMRILQPTIRLESCLGQ
LKRGQEKVRETKGCFLRPEAPQHDNVRL*PWSC
EL*ARNPGRNQHIYNHLPPPNAFTFTASAANCGH
YNRSCGTEAYPGESAQGATLCPRGNPHLLVKPP
LPLTSNQDSLLFQAVGSCLFWLRTGWGERARDP
QEGLSLRLRGRHNKGWGPAGGAGRSR*YQAQSPS
LSPQSHHGPHRGAHHRLFLGHRPALGRTSGFRS
IPELQSVCHVEGPENTGPAVGGGPGPGMPSGIP
GDVAAGRKGLKIRGRCPGTRD*GPRGRAGV*RRP
GPAGAAGGAGGGRRGLCAGRECSRDCADAAGLP
ARHEEARFGTRVGDRERGRIDGAAFQ*RLLRQQ
VRARRLMRESGGSAAALWGPQLEPDRVRPSAHRL
HGEGVGQPRGGAGPHGHPHLPPLLPIPRPQQAS
LSRGGAEP*GGGGGLPHRFARPEADPALLHHRA
LPAPAADAPGRPQRLQLRHRHAPGSVRRRSGKGR
GWGRGWGRGRAWGRGRGRARCGGGP*ARRSSGR
PAVKASSAAVSRALLCPLGLCGPWGWGGGSSCG
WLIKIDRVSQFYQRS*ARWLSPVMPALWEAEAGG
SLKPRSWRPARATQ*DPHLYKNKENLKI
Sequence HSD17B1.aAug05
AGTTAGGTGGGACTTGGGTTGGGGTAGAGTCAGGTTGTGACCTGCACTCCATTAGCCATGAGACCTCAGGCAAGTTCCTTGCTTTCTCTGAGTGCTTTCC
TTTCCTTTCCCTTTTCTTTTCTTTTTTGTTTTTCTTTTGTTTTGAGACGGAGTTTCACTCTTGTTGCCCAGCCTGGAGTGCAATAGCGCGGTCTCAGTTC
ACCACAACCTCTGCCTCCCGGGTTCAAGTGTAGCTAGGATTGCAGGCATGTGCCACCACGCCCAGCTAATTTTGTATTTTTAGTAGAGACGAGGTTTCTC
CATGTTGGTCAGACTGGTCTTGGACTCCCGACCTCAGGTGATCCGCCCGCCCTGGCCTCTCAGAGTGCTGGGATTACAGGCATGACCCACCCCATCCGGC
CCTCTGAGTCTTTCTTATCTGTAAAATGGGTATAATAATACCTATCTAATCGGTTTTAGTAATGGTGGAGATAATGCCTGAAAGTGCTAGTATAGAGGTT
TAATACCCAGCAAGGACTGTTATTAGAATGAATAGTAATATGACTACTGTCACATTTTGCAAATGTGTAAAGAAGAAAGAGGGCTAAGTGAATCAAAGGG
AGACAGCCCCACTCACCCTGTTCTGCCCCAGAGGACTGAGCGATCCCCACCATCTGGGAAGCTGCCTCACCAGGAGGTCCAGCTGGGGCTACAGGACTGG
CTACTTTGCTACAATGGCCCATCTTTCCTGAGCCCAGTGGAGGGTCCCAGGGGGGCAGAAGTCATTGATAGGGGCCAGTAGGGTTGTAGAGCCACTGTCT
GAACTTCTGTGGAGGTCTGGTGCAGGGGAGGTGTGAACAGATAGGAGGCTAGGTGAGGATGCAGCAGAGGAAGGGGGCAGGAGTGCCCCAGGAGGGGGCG
GTACCGAGGCAGGGATTCAGCTGGGTCTGAGAGGGAGGGAAGGCTGAGGGGGATGGCCCTTTGGAGTGGGCAGGGACATGACCACAGAAAGCCTGGGAAG
TGGAAACAGACAGGAGCCTGCTGGAGCTCTTGGAGCTTTGTGTGGGGCACTAGGGGAAGGAGGCAGGCACTCCACCCTGACCTGCCCCTACCTCTGGATG
AGGGTCTTCTCTGCCTGTTGGATGATGTGCTGCCCCTGCTCTTGGAGGAAGAGGCCTCCCAGCCACCCCCTCCTGCCAGTCGCCTTGCCCTGCTGACCCA
GACAGATCTGCTATCCCTACAGCATCCAGGAAGGCCACAGGGAGGGGGCCCAGGTGGCATGGGTTTCTGAGGCCTGGGATCTGCTCTCAGTCCCGCTCTC
GCCAACCCCCTTTGCCCCTCTGTGACAGTGTGTGTACATATGCGTGCATGTGTGTACCTGTGTGGTGTGGCTCACAAGGTCACCCCTGGGGGTGGGATAA
TAAGAGGTAAAGTGTGACCCCCTCTCTTCCGTACATTCATTTTTTCAAGCTTGTGAATATTCACATTGTTAATATAATCTTCTGCAGTCCCATGACTTCG
AATAGCACTTAGATGCCGATGACCCCCCCACCAAAGAAACCCCACTGTCTCCCACAGACTCCAGGTCTGTATCTCCAACTGCCTACTCAATACAACACGT
CCACCTGATACAATGAACTTAATGTGTCTAAACCCCAATTCCACATTTCCAGTCCTCCTACCGGCTCCTCCCCCAGTCTTCTCTACTTTACTATATGACA
ACTCCATTTTTCCCAATACTGGTTTTAAAAACCCTGGAGTAGTCCTTGACTCCAGTCTCTCTCACCTTTCAACCAATTCAGCAGCAAATACTGACAGCTC
TACCTTTGCTCATCAAAGCATATCCCCAAATCACCCTATTCCAGACTGCACACTGTCATCTCTCCCTTCAATTACAGCAGTAGCCTCCTAACTAGTTTTC
TTGATTCCACTCCTGCCCATCAGCAGTGAAATTACCCCAGAGCAGTTAAAATGATCTTTTAGGATGGGCACAGTGGCTCACGCCTGTAATACCAGCACTT
TGGGAGGTGGAGGCTGGCTGATCCCCTTAGGTTGGGAGTTCAAGACCATCCTCACCAACAGGGAGAAACCCCATCTCTACTAAAAATACAAAAATTAGCC
GGATGTGGTGGGACGTGTCTGTAATCCCAACTACTTGGGAGGCTGAGACAGGAGAACTGCTTGAACCCGGGAGGTGGAGGTTGCAGTGAGCCAGAATCGT
GCCACAGCACTCCAGCCTGGGCAAGAAGCGCGAAACTCCGTCTCAAAATAAAATAATCTTCTAAAAATGACAGGGCCAGGTGGGGTGGCACTTTTTTATA
ATCCGAGCACTTTGGGAGGCTGAGGTGGGCAGATCGCTTGACATCAGGGGTTTGAGACCAGCCTGGCCAACATGGTGAAACTCCGTCTCTACTAAAAATA
CTAAAAATTAGCTGGGCGTGGTGGCGGGTGCCTGTAATCCCAGCTACTCGGGAGGCTGATGCAGGAGAATCGCCTGAACCAGGCAGATGCAGGATGCAGT
GAGCCAAGATTAAGACACTGCACTCCAACCTAAGCAATACTTTGTCTCAAAAATAAAAAAAAGCCTGGGAAACAAAGTGAGACCCCGTCTCTACAAAAAA
GTCAAAAAATTAGCTGGGTATGGTGGCAGTGATGGCACACACCTGTAGTCCCGGCTACTTGGGAAGCTTTTTAATATTTTTTGCAGAGACCGGGTCTCAC
TCTGTTACCTGGCCTGGTCTTGAACTCCTGGGCTCCAACAATCCCCTTTCCTGGGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACCGTGCCCAGC
CTCAAAGCATATTTTAAAGGATAGAAATAAACAGCCATATGAAGAGATACAGACAGGGCGGTCTGGAAGGGTCCAGAGCAGGAGCTTCTATCTCCATAGA
GTTGGGGTTACGTCACCCTCTGGGCACATTCTGTCAGCCTCCACACGTTCAGCCCTCAGAAGCTCCCGAACCCTGTCCTTTGGGCCTTTTATGGAGAACT
CCATTGGCTGTCCATGACTGAAGCATGGACAACTGTGATAATGTGATTGGGCAAAAAGGGTCTGATCTAAGCCCAGCAAGGCCAGTCCAGATTCTTTGGG
CCTTTGTGCAGCATTCCTTTCTCCAGGGTATGGGGCAAGGACCCACTCTGGAATGAGGATCCTACAACCCACAATCAGATTAGAGTCCTGCCTTGGGCAG
CTGAAAAGAGGACAGGAGAAGGTCAGAGAGACGAAAGGCTGTTTTTTGAGGCCTGAGGCACCCCAACATGACAACGTAAGACTGTAACCATGGTCATGTG
AGTTATGAGCTAGGAACCCTGGACGAAACCAGCACATATACAATCATCTCCCACCTCCCAACGCCTTTACTTTCACAGCCTCTGCAGCAAACTGCGGTCA
CTATAATCGCTCCTGTGGCACAGAGGCATACCCAGGGGAATCTGCCCAGGGGGCCACTCTGTGCCCACGTGGGAACCCACACCTGCTTGTAAAGCCTCCC
CTCCCTCTGACCAGCAACCAGGACAGTTTGTTGTTCCAAGCAGTGGGCTCATGTCTGTTTTGGCTCAGAACAGGGTGGGGAGAGCGGGCCAGGGACCCGC
AGGAAGGCTTATCCTTGAGATTGCGTGGGAGACACAACAAGGGGTGGGGGCCCGCAGGCGGGGCGGGGCGAAGCAGGTGATATCAAGCCCAGAGCCCCAG
CCTCTCCCCACAGTCTCACCATGGCCCGCACCGTGGTGCTCATCACCGGCTGTTCCTCGGGCATCGGCCTGCACTTGGCCGTACGTCTGGCTTCAGATCC
ATCCCAGAGCTTCAAAGTGTATGCCACGTTGAGGGACCTGAAAACACAGGGCCGGCTGTGGGAGGCGGCCCGGGCCCTGGCATGCCCTCCGGGATCCCTG
GAGACGTTGCAGCTGGACGTAAGGGACTCAAAATCCGTGGCCGCTGCCCGGGAACGCGTGACTGAGGGCCGCGTGGACGTGCTGGTGTGTAACGCAGGCC
TGGGCCTGCTGGGGCCGCTGGAGGCGCTGGGGGAGGACGCCGTGGCCTCTGTGCTGGACGTGAATGTAGTAGGGACTGTGCGGATGCTGCAGGCCTTCCT
GCCAGACATGAAGAGGCGCGGTTCGGGACGCGTGTTGGTGACCGGGAGCGTGGGAGGATTGATGGGGCTGCCTTTCAATGACGTTTATTGCGCCAGCAAG
TTCGCGCTCGAAGGCTTATGCGAGAGTCTGGCGGTTCTGCTGCTGCCCTTTGGGGTCCACTTGAGCCTGATCGAGTGCGGCCCAGTGCACACCGCCTTCA
TGGAGAAGGTGTTGGGCAGCCCAGAGGAGGTGCTGGACCGCACGGACATCCACACCTTCCACCGCTTCTACCAATACCTCGCCCACAGCAAGCAAGTCTT
TCGCGAGGCGGCGCAGAACCCTGAGGAGGTGGCGGAGGTCTTCCTCACCGCTTTGCGCGCCCCGAAGCCGACCCTGCGCTACTTCACCACCGAGCGCTTC
CTGCCCCTGCTGCGGATGCGCCTGGACGACCCCAGCGGCTCCAACTACGTCACCGCCATGCACCGGGAAGTGTTCGGCGACGTTCCGGCAAAGGCCGAGG
CTGGGGCCGAGGCTGGGGGCGGGGCCGGGCCTGGGGCAGAGGACGAGGCCGGGCGCGGTGCGGTGGGGGACCCTGAGCTCGGCGATCCTCCGGCCGCCCC
GCAGTAAAGGCTTCCTCAGCCGCTGTCTCCCGCGCCCTTCTTTGTCCCCTGGGTCTGTGTGGTCCCTGGGGATGGGGCGGCGGTAGCAGCTGTGGGTGGC
TAATTAAGATAGATCGCGTTAGCCAGTTTTACCAGCGCAGCTAGGCGCGATGGCTGTCGCCTGTAATGCCAGCGCTTTGGGAGGCGGAGGCAGGAGGATC
GCTCAAGCCCCGGAGTTGGAGACCAGCCAGAGCAACACAGTGAGACCCCCATCTCTACAAAAATAAAGAAAATTTAAAAATCAG
- HSD17B1.F4 (forward primer)
- HSD17B1.R4 (reverse primer)
- Intron retention
Frame 0
S*VGLGLG*SQVVTCTPLAMRPQASSLLSLSAF
LSFPFSFLFCFSFVLRRSFTLVAQPGVQ*RGLSS
PQPLPPGFKCS*DCRHVPPRPANFVFLVETRFL
HVGQTGLGLPTSGDPPALASQSAGITGMTHPIR
PSESFLSVKWV**YLSNRF**WWR*CLKVLV*RF
NTQQGLLLE*IVI*LLSHFANV*RRKRAK*IKG
RQPHSPCSAPED*AIPTIWEAASPGGPAGATGL
ATLLQWPIFPEPSGGSQGGRSH**GPVGL*SHCL
NFCGGLVQGRCEQIGG*VRMQQRKGAGVPQEGA
VPRQGFSWV*EGGKAEGDGPLEWAGT*PQKAWE
VETDRSLLELLELCVGH*GKEAGTPP*PAPTSG*
GSSLPVG*CAAPALGGRGLPATPSCQSPCPADP
DRSAIPTASRKATGRGPRWHGFLRPGICSQSRS
RQPPLPLCDSVCTYACMCVPVWCGSQGHPWGWDN
KR*SVTPSLPYIHFFKLVNIHIVNIIFCSPMTS
NST*MPMTPPPKKPHCLPQTPGLYLQLPTQYNT
ST*YNELNVSKPQFHISSPPTGSSPSLLYFTI*Q
LHFSQYWF*KPWSSP*LQSLSPFNQFSSKY*QL
YLCSSKHIPKSPYSRLHTVISPFNYSSSLLTSF
LDSTPAHQQ*NYPRAVKMIF*DGHSGSRL*YQHF
GRWRLADPLRLGVQDHPHQQGETPSLLKIQKLA
GCGGTCL*SQLLGRLRQENCLNPGGGGCSEPES
CHSTPAWARSAKLRLKIK*SSKNDRARWGGTFL*
SEHFGRLRWADRLTSGV*DQPGQHGETPSLLKI
LKISWAWWRVPVIPATREADAGESPEPGRCRMQ
*AKIKTLHSNLSNTLSQK*KKAWETK*DPVSTKK
SKN*LGMVAVMAHTCSPGYLGSFLIFFAETGSH
SVTWPGLELLGSNNPLSWASQSAGITGMSHRAQ
PQSIF*RIEINSHMKRYRQGGLEGSRAGASISIE
LGLRHPLGTFCQPPHVQPSEAPEPCPLGLLWRT
PLAVHD*SMDNCDNVIGQKGSDLSPARPVQILW
AFVQHSFLQGMGQGPTLE*GSYNPQSD*SPALGS
*KEDRRRSERRKAVF*GLRHPNMTT*DCNHGHV
SYELGTLDETSTYTIISHLPTPLLSQPLQQTAV
TIIAPVAQRHTQGNLPRGPLCAHVGTHTCL*SLP
SL*PATRTVCCSKQWAHVCFGSEQGGESGPGTR
RKAYP*DCVGDTTRGGGPQAGRGEAGDIKPRAP
ASPHSLTMARTVVLITGCSSGIGLHLAVRLASDP
SQSFKVYATLRDLKTQGRLWEAARALACPPGSL
ETLQLDVRDSKSVAAARERVTEGRVDVLVCNAG
LGLLGPLEALGEDAVASVLDVNVVGTVRMLQAFL
PDMKRRGSGRVLVTGSVGGLMGLPFNDVYCASK
FALEGLCESLAVLLLPFGVHLSLIECGPVHTAF
MEKVLGSPEEVLDRTDIHTFHRFYQYLAHSKQVF
REAAQNPEEVAEVFLTALRAPKPTLRYFTTERF
LPLLRMRLDDPSGSNYVTAMHREVFGDVPAKAE
AGAEAGGGAGPGAEDEAGRGAVGDPELGDPPAAP
Q*RLPQPLSPAPFFVPWVCVVPGDGAAVAAVGG
*LR*IALASFTSAARRDGCRL*CQRFGRRRQED
RSSPGVGDQPEQHSETPISTKIKKI*KS
Frame 1
VRWDLGWGRVRL*PALH*P*DLRQVPCFL*VLS
FPFPFLFFFVFLLF*DGVSLLLPSLECNSAVSV
HHNLCLPGSSVARIAGMCHHAQLILYF**RRGFS
MLVRLVLDSRPQVIRPPWPLRVLGLQA*PTPSG
PLSLSYL*NGYNNTYLIGFSNGGDNA*KC*YRG
LIPSKDCY*NE**YDYCHILQMCKEERGLSESKG
DSPTHPVLPQRTERSPPSGKLPHQEVQLGLQDW
LLCYNGPSFLSPVEGPRGAEVIDRGQ*GCRATV
*TSVEVWCRGGVNR*EAR*GCSRGRGQECPRRGR
YRGRDSAGSEREGRLRGMALWSGQGHDHRKPGK
WKQTGACWSSWSFVWGTRGRRQALHPDLPLPLD
EGLLCLLDDVLPLLLEEEASQPPPPASRLALLTQ
TDLLSLQHPGRPQGGGPGGMGF*GLGSALSPAL
ANPLCPSVTVCVHMRACVYLCGVAHKVTPGGGI
IRGKV*PPLFRTFIFSSL*IFTLLI*SSAVP*LR
IALRCR*PPHQRNPTVSHRLQVCISNCLLNTTR
PPDTMNLMCLNPNSTFPVLLPAPPPVFSTLLYD
NSIFPNTGFKNPGVVLDSSLSHLSTNSAANTDSS
TFAHQSISPNHPIPDCTLSSLPSITAVAS*LVF
LIPLLPISSEITPEQLK*SFRMGTVAHACNTST
LGGGGWLIPLGWEFKTILTNREKPHLY*KYKN*P
DVVGRVCNPNYLGG*DRRTA*TREVEVAVSQNR
ATALQPGQEARNSVSK*NNLLKMTGPGGVALFY
NPSTLGG*GGQIA*HQGFETSLANMVKLRLY*KY
*KLAGRGGGCL*SQLLGRLMQENRLNQADAGCS
EPRLRHCTPT*AILCLKNKKKPGKQSETPSLQK
SQKISWVWWQ*WHTPVVPATWEAF*YFLQRPGLT
LLPGLVLNSWAPTIPFPGPPKVLGLQA*ATVPS
LKAYFKG*K*TAI*RDTDRAVWKGPEQELLSP*
SWGYVTLWAHSVSLHTFSPQKLPNPVLWAFYGEL
HWLSMTEAWTTVIM*LGKKGLI*AQQGQSRFFG
PLCSIPFSRVWGKDPLWNEDPTTHNQIRVLPWA
AEKRTGEGQRDERLFFEA*GTPT*QRKTVTMVM*
VMS*EPWTKPAHIQSSPTSQRLYFHSLCSKLRS
L*SLLWHRGIPRGICPGGHSVPTWEPTPACKAS
PPSDQQPGQFVVPSSGLMSVLAQNRVGRAGQGPA
GRLILEIAWETQQGVGARRRGGAKQVISSPEPQ
PLPTVSPWPAPWCSSPAVPRASACTWPYVWLQI
HPRASKCMPR*GT*KHRAGCGRRPGPWHALRDPW
RRCSWT*GTQNPWPLPGNA*LRAAWTCWCVTQA
WACWGRWRRWGRTPWPLCWT*M**GLCGCCRPS
CQT*RGAVRDACW*PGAWED*WGCLSMTFIAPAS
SRSKAYARVWRFCCCPLGST*A*SSAAQCTPPS
WRRCWAAQRRCWTARTSTPSTASTNTSPTASKS
FARRRRTLRRWRRSSSPLCAPRSRPCATSPPSAS
CPCCGCAWTTPAAPTTSPPCTGKCSATFRQRPR
LGPRLGAGPGLGQRTRPGAVRWGTLSSAILRPP
RSKGFLSRCLPRPSLSPGSVWSLGMGRR*QLWVA
N*DRSR*PVLPAQLGAMAVACNASALGGGGRRI
AQAPELETSQSNTVRPPSLQK*RKFKNQ
Frame 2
LGGTWVGVESGCDLHSISHETSGKFLAFSECFP
FLSLFFSFLFFFCFETEFHSCCPAWSAIARSQF
TTTSASRVQV*LGLQACATTPS*FCIFSRDEVS
PCWSDWSWTPDLR*SARPGLSECWDYRHDPPHPA
L*VFLICKMGIIIPI*SVLVMVEIMPESASIEV
*YPARTVIRMNSNMTTVTFCKCVKKKEG*VNQR
ETAPLTLFCPRGLSDPHHLGSCLTRRSSWGYRTG
YFATMAHLS*AQWRVPGGQKSLIGASRVVEPLS
ELLWRSGAGEV*TDRRLGEDAAEEGGRSAPGGG
GTEAGIQLGLRGREG*GGWPFGVGRDMTTESLGS
GNRQEPAGALGALCGALGEGGRHSTLTCPYLWM
RVFSACWMMCCPCSWRKRPPSHPLLPVALPC*P
RQICYPYSIQEGHREGAQVAWVSEAWDLLSVPLS
PTPFAPL*QCVYICVHVCTCVVWLTRSPLGVG*
*EVKCDPLSSVHSFFQACEYSHC*YNLLQSHDF
E*HLDADDPPTKETPLSPTDSRSVSPTAYSIQHV
HLIQ*T*CV*TPIPHFQSSYRLLPQSSLLYYMT
TPFFPILVLKTLE*SLTPVSLTFQPIQQQILTA
LPLLIKAYPQITLFQTAHCHLSLQLQQ*PPN*FS
*FHSCPSAVKLPQSS*NDLLGWAQWLTPVIPAL
WEVEAG*SP*VGSSRPSSPTGRNPISTKNTKIS
RMWWDVSVIPTTWEAETGELLEPGRWRLQ*ARIV
PQHSSLGKKRETPSQNKIIF*K*QGQVGWHFFI
IRALWEAEVGRSLDIRGLRPAWPTW*NSVSTKN
TKN*LGVVAGACNPSYSGG*CRRIA*TRQMQDAV
SQD*DTALQPKQYFVSKIKKSLGNKVRPRLYKK
VKKLAGYGGSDGTHL*SRLLGKLFNIFCRDRVS
LCYLAWS*TPGLQQSPFLGLPKCWDYRHEPPCPA
SKHILKDRNKQPYEEIQTGRSGRVQSRSFYLHR
VGVTSPSGHILSASTRSALRSSRTLSFGPFMEN
SIGCP*LKHGQL**CDWAKRV*SKPSKASPDSLG
LCAAFLSPGYGARTHSGMRILQPTIRLESCLGQ
LKRGQEKVRETKGCFLRPEAPQHDNVRL*PWSC
EL*ARNPGRNQHIYNHLPPPNAFTFTASAANCGH
YNRSCGTEAYPGESAQGATLCPRGNPHLLVKPP
LPLTSNQDSLLFQAVGSCLFWLRTGWGERARDP
QEGLSLRLRGRHNKGWGPAGGAGRSR*YQAQSPS
LSPQSHHGPHRGAHHRLFLGHRPALGRTSGFRS
IPELQSVCHVEGPENTGPAVGGGPGPGMPSGIP
GDVAAGRKGLKIRGRCPGTRD*GPRGRAGV*RRP
GPAGAAGGAGGGRRGLCAGRECSRDCADAAGLP
ARHEEARFGTRVGDRERGRIDGAAFQ*RLLRQQ
VRARRLMRESGGSAAALWGPLEPDRVRPSAHRLH
GEGVGQPRGGAGPHGHPHLPPLLPIPRPQQASL
SRGGAEP*GGGGGLPHRFARPEADPALLHHRAL
PAPAADAPGRPQRLQLRHRHAPGSVRRRSGKGRG
WGRGWGRGRAWGRGRGRARCGGGP*ARRSSGRP
AVKASSAAVSRALLCPLGLCGPWGWGGGSSCGW
LIKIDRVSQFYQRS*ARWLSPVMPALWEAEAGGS
LKPRSWRPARATQ*DPHLYKNKENLKI
Sequence HSD17B1.dAug05
AGGACCCACTCTGGAATGAGGATCCTACAACCCACAATCAGATTAGAGTCCTGCCTTGGGCAGCTGAAAAGAGGACAGGAGAAGGTCAGAGAGACGAAAG
GCTGTTTTTTGAGGCCTGAGGCACCCCAACATGACAACGTAAGACTGTAACCATGGTCATGTGAGTTATGAGCTAGGAACCCTGGACGAAACCAGCACAT
ATACAATCATCTCCCACCTCCCAACGCCTTTACTTTCACAGCCTCTGCAGCAAACTGCGGTCACTATAATCGCTCCTGTGGCACAGAGGCATACCCAGGG
GAATCTGCCCAGGGGGCCACTCTGTGCCCACGTGGGAACCCACACCTGCTTGTAAAGCCTCCCCTCCCTCTGACCAGCAACCAGGACAGTTTGTTGTTCC
AAGCAGTGGGCTCATGTCTGTTTTGGCTCAGAACAGGGTGGGGAGAGCGGGCCAGGGACCCGCAGGAAGGCTTATCCTTGAGATTGCGTGGGAGACACAA
CAAGGGGTGGGGGCCCGCAGGCGGGGCGGGGCGAAGCAGGTGATATCAAGCCCAGAGCCCCAGCCTCTCCCCACAGTCTCACCATGGCCCGCACCGTGGT
GCTCATCACCGGCTGTTCCTCGGGCATCGGCCTGCACTTGGCCGTACGTCTGGCTTCAGATCCATCCCAGAGCTTCAAAGGTATAGATAGGCAGGGACAG
GGAGGGAGAGAAGGGAGGAGCCCTTGGAGGCCAGAAGGGAAGTCAGATCTTCCTCCTCTCCCAAAACCTCCAGTGTATGCCACGTTGAGGGACCTGAAAA
CACAGGGCCGGCTGTGGGAGGCGGCCCGGGCCCTGGCATGCCCTCCGGGATCCCTGGAGACGTTGCAGCTGGACGTAAGGGACTCAAAATCCGTGGCCGC
TGCCCGGGAACGCGTGACTGAGGGCCGCGTGGACGTGCTGGTGTGTAACGCAGGCCTGGGCCTGCTGGGGCCGCTGGAGGCGCTGGGGGAGGACGCCGTG
GCCTCTGTGCTGGACGTGAATGTAGTAGGGACTGTGCGGATGCTGCAGGCCTTCCTGCCAGACATGAAGAGGCGCGGTTCGGGACGCGTGTTGGTGACCG
GGAGCGTGGGAGGATTGATGGGTGAGTGGTAGGGAGTGGCCTCGGCAGCTCCAGATTCTTTGTGTGCGGAGCTGAGCCTTGAAGGCAGGTTCCGCGGGGG
GGGTGGAGTGGGGTGCCGTCAGCTTGGAGGGGCACCGTCTGCCCGGGGATGACCCCCTGGCCGCTGCGCCTCAGGAACCTCGTCTCCCCACCTAAGGGCT
GCCTTTCAATGACGTTTATTGCGCCAGCAAGTTCGCGCTCGAAGGCTTATGCGAGAGTCTGGCGGTTCTGCTGCTGCCCTTTGGGGTCCACTTGAGCCTG
ATCGAGTGCGGCCCAGTGCACACCGCCTTCATGGAGAAGGTGTTGGGCAGCCCAGAGGAGGTGCTGGACCGCACGGACATCCACACCTTCCACCGCTTCT
ACCAATACCTCGCCCACAGCAAGCAAGTCTTTCGCGAGGCGGCGCAGAACCCTGAGGAGGTGGCGGAGGTCTTCCTCACCGCTTTGCGCGCCCCGAAGCC
GACCCTGCGCTACTTCACCACCGAGCGCTTCCTGCCCCTGCTGCGGATGCGCCTGGACGACCCCAGCGGCTCCAACTACGTCACCGCCATGCACCGGGAA
GTGTTCGGCGACGTTCCGGCAAAGGCCGAGGCTGGGGCCGAGGCTGGGGGCGGGGCCGGGCCTGGGGCAGAGGACGAGGCCGGGCGCGGTGCGGTGGGGG
ACCCTGAGCTCGGCGATCCTCCGGCCGCCCCGCAGTAAAGGCTTCCTCAGCCGCTGTCTCCCGCGCCCTTCTTTGTCCCCTGGGTCTGTGTGGTCCCTGG
GGATGGGGCGGCGGTAGCAGCTGTGGGTGGCTAATTAAGATAGATCGCGTTAGCCAGTTTTACCAGCGCAGCTAGGCGCGATGGCTGTCGCCTGTAATGC
CAGCGCTTTGGGAGGCGGAGGCAGGAGGATCGCTCAAGCCCCGGAGTTGGAGACCAGCCAGAGCAACACAGTGAGACCCCCATCTCTACAAAAATAAAGA
AAATTTAAAAATCAG
- HSD17B1.F4 (forward primer)
- HSD17B1.R4 (reverse primer)
- Intron retention
Frame 0
RTHSGMRILQPTIRLESCLGQLKRGQEKVRETK
GCFLRPEAPQHDNVRL*PWSCEL*ARNPGRNQHI
YNHLPPPNAFTFTASAANCGHYNRSCGTEAYPG
ESAQGATLCPRGNPHLLVKPPLPLTSNQDSLLF
QAVGSCLFWLRTGWGERARDPQEGLSLRLRGRHN
KGWGPAGGAGRSR*YQAQSPSLSPQSHHGPHRG
AHHRLFLGHRPALGRTSGFRSIPELQRYR*AGT
GRERREEPLEARREVRSSSSPKTSSVCHVEGPEN
TGPAVGGGPGPGMPSGIPGDVAAGRKGLKIRGR
CPGTRD*GPRGRAGV*RRPGPAGAAGGAGGGRR
GLCAGRECSRDCADAAGLPARHEEARFGTRVGDR
ERGRIDG*VVGSGLGSSRFFVCGAEP*RQVPRG
GWSGVPSAWRGTVCPGMTPWPLRLRNLVSPPKG
CLSMTFIAPASSRSKAYARVWRFCCCPLGST*A*
SSAAQCTPPSWRRCWAAQRRCWTARTSTPSTAS
TNTSPTASKSFARRRRTLRRWRRSSSPLCAPRS
RPCATSPPSASCPCCGCAWTTPAAPTTSPPCTGK
CSATFRQRPRLGPRLGAGPGLGQRTRPGAVRWG
TLSSAILRPPRSKGFLSRCLPRPSLSPGSVWSL
GMGRR*QLWVAN*DRSR*PVLPAQLGAMAVACNA
SALGGGGRRIAQAPELETSQSNTVRPPSLQK*R
KFKNQ
Frame 1
GPTLE*GSYNPQSD*SPALGS*KEDRRRSERRK
AVF*GLRHPNMTT*DCNHGHVSYELGTLDETST
YTIISHLPTPLLSQPLQQTAVTIIAPVAQRHTQG
NLPRGPLCAHVGTHTCL*SLPSL*PATRTVCCS
KQWAHVCFGSEQGGESGPGTRRKAYP*DCVGDT
TRGGGPQAGRGEAGDIKPRAPASPHSLTMARTVV
LITGCSSGIGLHLAVRLASDPSQSFKGIDRQGQ
GGREGRSPWRPEGKSDLPPLPKPPVYATLRDLK
TQGRLWEAARALACPPGSLETLQLDVRDSKSVAA
ARERVTEGRVDVLVCNAGLGLLGPLEALGEDAV
ASVLDVNVVGTVRMLQAFLPDMKRRGSGRVLVT
GSVGGLMGEW*GVASAAPDSLCAELSLEGRFRGG
GGVGCRQLGGAPSARG*PPGRCASGTSSPHLRA
AFQ*RLLRQQVRARRLMRESGGSAAALWGPLEP
DRVRPSAHRLHGEGVGQPRGGAGPHGHPHLPPLL
PIPRPQQASLSRGGAEP*GGGGGLPHRFARPEA
DPALLHHRALPAPAADAPGRPQRLQLRHRHAPG
SVRRRSGKGRGWGRGWGRGRAWGRGRGRARCGGG
P*ARRSSGRPAVKASSAAVSRALLCPLGLCGPW
GWGGGSSCGWLIKIDRVSQFYQRS*ARWLSPVM
PALWEAEAGGSLKPRSWRPARATQ*DPHLYKNKE
NLKI
Frame 2
DPLWNEDPTTHNQIRVLPWAAEKRTGEGQRDER
LFFEA*GTPT*QRKTVTMVM*VMS*EPWTKPAH
IQSSPTSQRLYFHSLCSKLRSL*SLLWHRGIPR
GICPGGHSVPTWEPTPACKASPPSDQQPGQFVVP
SSGLMSVLAQNRVGRAGQGPAGRLILEIAWETQ
QGVGARRRGGAKQVISSPEPQPLPTVSPWPAPW
CSSPAVPRASACTWPYVWLQIHPRASKV*IGRDR
EGEKGGALGGQKGSQIFLLSQNLQCMPR*GT*K
HRAGCGRRPGPWHALRDPWRRCSWT*GTQNPWP
LPGNA*LRAAWTCWCVTQAWACWGRWRRWGRTPW
PLCWT*M**GLCGCCRPSCQT*RGAVRDACW*P
GAWED*WVSGREWPRQLQILCVRS*ALKAGSAG
GVEWGAVSLEGHRLPGDDPLAAAPQEPRLPT*GL
PFNDVYCASKFALEGLCESLAVLLLPFGVHLSL
IECGPVHTAFMEKVLGSPEEVLDRTDIHTFHRF
YQYLAHSKQVFREAAQNPEEVAEVFLTALRAPKP
TLRYFTTERFLPLLRMRLDDPSGSNYVTAMHRE
VFGDVPAKAEAGAEAGGGAGPGAEDEAGRGAVG
DPELGDPPAAPQ*RLPQPLSPAPFFVPWVCVVPG
DGAAVAAVGG*LR*IALASFTSAARRDGCRL*C
QRFGRRRQEDRSSPGVGDQPEQHSETPISTKIK
KI*KS
Sequence HSD17B1.shed1.aAug05
AGGACCCACTCTGGAATGAGGATCCTACAACCCACAATCAGATTAGAGTCCTGCCTTGGGCAGCTGAAAAGAGGACAGGAGAAGGTCAGAGAGACGAAAG
GCTGTTTTTTGAGGCCTGAGGCACCCCAACATGACAACGTAAGACTGTAACCATGGTCATGTGAGTTATGAGCTAGGAACCCTGGACGAAACCAGCACAT
ATACAATCATCTCCCACCTCCCAACGCCTTTACTTTCACAGCCTCTGCAGCAAACTGCGGTCACTATAATCGCTCCTGTGGCACAGAGGCATACCCAGGG
GAATCTGCCCAGGGGGCCACTCTGTGCCCACGTGGGAACCCACACCTGCTTGTAAAGCCTCCCCTCCCTCTGACCAGCAACCAGGACAGTTTGTTGTTCC
AAGCAGTGGGCTCATGTCTGTTTTGGCTCAGAACAGGGTGGGGAGAGCGGGCCAGGGACCCGCAGGAAGGCTTATCCTTGAGATTGCGTGGGAGACACAA
CAAGGGGTGGGGGCCCGCAGGCGGGGCGGGGCGAAGCAGGTGATATCAAGCCCAGAGCCCCAGCCTCTCCCCACAGTCTCACCATGGCCCGCACCGTGGT
GCTCATCACCGGCTGTTCCTCGGGCATCGGCCTGCACTTGGCCGTACGTCTGGCTTCAGATCCATCCCAGAGCTTCAAAGGTATAGATAGGCAGGGACAG
GGAGGGAGAGAAGGGAGGAGCCCTTGGAGGCCAGAAGGGAAGTCAGATCTTCCTCCTCTCCCAAAACCTCCAGTGTATGCCACGTTGAGGGACCTGAAAA
CACAGGGCCGGCTGTGGGAGGCGGCCCGGGCCCTGGCATGCCCTCCGGGATCCCTGGAGACGTTGCAGCTGGACGTAAGGGACTCAAAATCCGTGGCCGC
TGCCCGGGAACGCGTGACTGAGGGCCGCGTGGACGTGCTGGGTGAGCCTCCTGGAAGCATATGGGCTCCTAGGAGCCTTCTCCGCCCTGCGTTGAAACCA
ACATGTTCCCAGGCCCAGGGAGCACGAGGGGACAGGCCGTGCTGAGGGTGATGCTGAGGCGGGCTGGTCGGGCCTCTTGTCTCCGCAGTGTGTAACGCAG
GCCTGGGCCTGCTGGGGCCGCTGGAGGCGCTGGGGGAGGACGCCGTGGCCTCTGTGCTGGACGTGAATGTAGTAGGGACTGTGCGGATGCTGCAGGCCTT
CCTGCCAGACATGAAGAGGCGCGGTTCGGGACGCGTGTTGGTGACCGGGAGCGTGGGAGGATTGATGGGTGAGTGGTAGGGAGTGGCCTCGGCAGCTCCA
GATTCTTTGTGTGCGGAGCTGAGCCTTGAAGGCAGGTTCCGCGGGGGGGGTGGAGTGGGGTGCCGTCAGCTTGGAGGGGCACCGTCTGCCCGGGGATGAC
CCCCTGGCCGCTGCGCCTCAGGAACCTCGTCTCCCCACCTAAGGGCTGCCTTTCAATGACGTTTATTGCGCCAGCAAGTTCGCGCTCGAAGGCTTATGCG
AGAGTCTGGCGGTTCTGCTGCTGCCCTTTGGGGTCCAGTGAGTCAACACCCCCGTTCCCCGAACCCTCTTAACTCTGACCTAGAGACGCCGAGCACCCTG
TCCTGCGGGAGCCGCTCTGGGGCGATCTCCCTGGCCCTCTCTGCCCGGCTCACATTAGCTGTGTGCCAGGCACTTGGGCTGGGCGCATGGCACGCATTGT
GCCACCTGCTGACCTAGCTGCTGAAGTGTTGGTATTGTTATGGGGAAGCTCCAGCCCAGAGAGGTTAGGTGACTGGCCCAAGGTCACACAGCGGCCAGGG
ACCCCGCTAGATTCGAATCCTGACACCAGGGCTCCCTTGTAGCCTCAGATGGATTTGGGAGGGCTGCTCCGGCAGGAACCCGCGTTTCAAATGTTCTGGT
TATCCCCAGCGCCCTTTCCGCCTCACTTCCCAGCGCAGCGGTGCTGCTCGCGGTCGGGGGCCGGGACGTGGTTGGGGCTGGGACTGGGGTTGGGGCTGGG
ACTGGGGCCTGGCTGGCGTCCGCCCCCTCCCACTCGTTGCTCTCCGGCCAGCAGCTTGAGCCTGATCGAGTGCGGCCCAGTGCACACCGCCTTCATGGAG
AAGGTGTTGGGCAGCCCAGAGGAGGTGCTGGACCGCACGGACATCCACACCTTCCACCGCTTCTACCAATACCTCGCCCACAGCAAGCAAGTCTTTCGCG
AGGCGGCGCAGAACCCTGAGGAGGTGGCGGAGGTGAGCGCCGGGCTGGACTCCAGGAGTGGGGGCGGTGCGTCCTCCGGCGCGCAGCGGTGGCCACAGCT
CTCCTCCCGCCGCCGCAGGTCTTCCTCACCGCTTTGCGCGCCCCGAAGCCGACCCTGCGCTACTTCACCACCGAGCGCTTCCTGCCCCTGCTGCGGATGC
GCCTGGACGACCCCAGCGGCTCCAACTACGTCACCGCCATGCACCGGGAAGTGTTCGGCGACGTTCCGGCAAAGGCCGAGGCTGGGGCCGAGGCTGGGGG
CGGGGCCGGGCCTGGGGCAGAGGACGAGGCCGGGCGCGGTGCGGTGGGGGACCCTGAGCTCGGCGATCCTCCGGCCGCCCCGCAGTAAAGGCTTCCTCAG
CCGCTGTCTCCCGCGCCCTTCTTTGTCCCCTGGGTCTGTGTGGTCCCTGGGGATGGGGCGGCGGTAGCAGCTGTGGGTGGCTAATTAAGATAGATCGCGT
TAGCCAGTTTTACCAGCGCAGCTAGGCGCGATGGCTGTCGCCTGTAATGCCAGCGCTTTGGGAGGCGGAGGCAGGAGGATCGCTCAAGCCCCGGAGTTGG
AGACCAGCCAGAGCAACACAGTGAGACCCCCATCTCTACAAAAATAAAGAAAATTT
- HSD17B1.F4 (forward primer)
- HSD17B1.R4 (reverse primer)
- Intron retention
Frame 0
RTHSGMRILQPTIRLESCLGQLKRGQEKVRETK
GCFLRPEAPQHDNVRL*PWSCEL*ARNPGRNQHI
YNHLPPPNAFTFTASAANCGHYNRSCGTEAYPG
ESAQGATLCPRGNPHLLVKPPLPLTSNQDSLLF
QAVGSCLFWLRTGWGERARDPQEGLSLRLRGRHN
KGWGPAGGAGRSR*YQAQSPSLSPQSHHGPHRG
AHHRLFLGHRPALGRTSGFRSIPELQRYR*AGT
GRERREEPLEARREVRSSSSPKTSSVCHVEGPEN
TGPAVGGGPGPGMPSGIPGDVAAGRKGLKIRGR
CPGTRD*GPRGRAG*ASWKHMGS*EPSPPCVET
NMFPGPGSTRGQAVLRVMLRRAGRASCLRSV*RR
PGPAGAAGGAGGGRRGLCAGRECSRDCADAAGL
PARHEEARFGTRVGDRERGRIDG*VVGSGLGSS
RFFVCGAEP*RQVPRGGWSGVPSAWRGTVCPGMT
PWPLRLRNLVSPPKGCLSMTFIAPASSRSKAYA
RVWRFCCCPLGSSESTPPFPEPS*L*PRDAEHP
VLREPLWGDLPGPLCPAHISCVPGTWAGRMARIV
PPADLAAEVLVLLWGSSSPERLGDWPKVTQRPG
TPLDSNPDTRAPL*PQMDLGGLLRQEPAFQMFW
LSPAPFPPHFPAQRCCSRSGAGTWLGLGLGLGLG
LGPGWRPPPPTRCSPASSLSLIECGPVHTAFME
KVLGSPEEVLDRTDIHTFHRFYQYLAHSKQVFR
EAAQNPEEVAEVSAGLDSRSGGGASSGAQRWPQL
SSRRRRSSSPLCAPRSRPCATSPPSASCPCCGC
AWTTPAAPTTSPPCTGKCSATFRQRPRLGPRLG
AGPGLGQRTRPGAVRWGTLSSAILRPPRSKGFLS
RCLPRPSLSPGSVWSLGMGRR*QLWVAN*DRSR
*PVLPAQLGAMAVACNASALGGGGRRIAQAPEL
ETSQSNTVRPPSLQK*RKF
Frame 1
GPTLE*GSYNPQSD*SPALGS*KEDRRRSERRK
AVF*GLRHPNMTT*DCNHGHVSYELGTLDETST
YTIISHLPTPLLSQPLQQTAVTIIAPVAQRHTQG
NLPRGPLCAHVGTHTCL*SLPSL*PATRTVCCS
KQWAHVCFGSEQGGESGPGTRRKAYP*DCVGDT
TRGGGPQAGRGEAGDIKPRAPASPHSLTMARTVV
LITGCSSGIGLHLAVRLASDPSQSFKGIDRQGQ
GGREGRSPWRPEGKSDLPPLPKPPVYATLRDLK
TQGRLWEAARALACPPGSLETLQLDVRDSKSVAA
ARERVTEGRVDVLGEPPGSIWAPRSLLRPALKP
TCSQAQGARGDRPC*G*C*GGLVGPLVSAVCNA
GLGLLGPLEALGEDAVASVLDVNVVGTVRMLQAF
LPDMKRRGSGRVLVTGSVGGLMGEW*GVASAAP
DSLCAELSLEGRFRGGGGVGCRQLGGAPSARG*
PPGRCASGTSSPHLRAAFQ*RLLRQQVRARRLMR
ESGGSAAALWGPVSQHPRSPNPLNSDLETPSTL
SCGSRSGAISLALSARLTLAVCQALGLGAWHAL
CHLLT*LLKCWYCYGEAPAQRG*VTGPRSHSGQG
PR*IRILTPGLPCSLRWIWEGCSGRNPRFKCSG
YPQRPFRLTSQRSGAARGRGPGRGWGWDWGWGW
DWGLAGVRPLPLVALRPAA*A*SSAAQCTPPSWR
RCWAAQRRCWTARTSTPSTASTNTSPTASKSFA
RRRRTLRRWRR*APGWTPGVGAVRPPARSGGHS
SPPAAAGLPHRFARPEADPALLHHRALPAPAADA
PGRPQRLQLRHRHAPGSVRRRSGKGRGWGRGWG
RGRAWGRGRGRARCGGGP*ARRSSGRPAVKASS
AAVSRALLCPLGLCGPWGWGGGSSCGWLIKIDRV
SQFYQRS*ARWLSPVMPALWEAEAGGSLKPRSW
RPARATQ*DPHLYKNKEN
Frame 2
DPLWNEDPTTHNQIRVLPWAAEKRTGEGQRDER
LFFEA*GTPT*QRKTVTMVM*VMS*EPWTKPAH
IQSSPTSQRLYFHSLCSKLRSL*SLLWHRGIPR
GICPGGHSVPTWEPTPACKASPPSDQQPGQFVVP
SSGLMSVLAQNRVGRAGQGPAGRLILEIAWETQ
QGVGARRRGGAKQVISSPEPQPLPTVSPWPAPW
CSSPAVPRASACTWPYVWLQIHPRASKV*IGRDR
EGEKGGALGGQKGSQIFLLSQNLQCMPR*GT*K
HRAGCGRRPGPWHALRDPWRRCSWT*GTQNPWP
LPGNA*LRAAWTCWVSLLEAYGLLGAFSALR*NQ
HVPRPREHEGTGRAEGDAEAGWSGLLSPQCVTQ
AWACWGRWRRWGRTPWPLCWT*M**GLCGCCRP
SCQT*RGAVRDACW*PGAWED*WVSGREWPRQLQ
ILCVRS*ALKAGSAGGVEWGAVSLEGHRLPGDD
PLAAAPQEPRLPT*GLPFNDVYCASKFALEGLC
ESLAVLLLPFGVQ*VNTPVPRTLLTLT*RRRAPC
PAGAALGRSPWPSLPGSH*LCARHLGWAHGTHC
ATC*PSC*SVGIVMGKLQPREVR*LAQGHTAAR
DPARFES*HQGSLVASDGFGRAAPAGTRVSNVLV
IPSALSASLPSAAVLLAVGGRDVVGAGTGVGAG
TGAWLASAPSHSLLSGQQLEPDRVRPSAHRLHG
EGVGQPRGGAGPHGHPHLPPLLPIPRPQQASLSR
GGAEP*GGGGGERRAGLQEWGRCVLRRAAVATA
LLPPPQVFLTALRAPKPTLRYFTTERFLPLLRM
RLDDPSGSNYVTAMHREVFGDVPAKAEAGAEAGG
GAGPGAEDEAGRGAVGDPELGDPPAAPQ*RLPQ
PLSPAPFFVPWVCVVPGDGAAVAAVGG*LR*IA
LASFTSAARRDGCRL*CQRFGRRRQEDRSSPGVG
DQPEQHSETPISTKIKKI
Sequence HSD17B1.gAug05
AGTTAGGTGGGACTTGGGTTGGGGTAGAGTCAGGTTGTGACCTGCACTCCATTAGCCATGAGACCTCAGGCAAGTTCCTTGCTTTCTCTGAGTGCTTTCC
TTTCCTTTCCCTTTTCTTTTCTTTTTTGTTTTTCTTTTGTTTTGAGACGGAGTTTCACTCTTGTTGCCCAGCCTGGAGTGCAATAGCGCGGTCTCAGTTC
ACCACAACCTCTGCCTCCCGGGTTCAAGTGTAGCTAGGATTGCAGGCATGTGCCACCACGCCCAGCTAATTTTGTATTTTTAGTAGAGACGAGGTTTCTC
CATGTTGGTCAGACTGGTCTTGGACTCCCGACCTCAGGTGATCCGCCCGCCCTGGCCTCTCAGAGTGCTGGGATTACAGGCATGACCCACCCCATCCGGC
CCTCTGAGTCTTTCTTATCTGTAAAATGGGTATAATAATACCTATCTAATCGGTTTTAGTAATGGTGGAGATAATGCCTGAAAGTGCTAGTATAGAGGTT
TAATACCCAGCAAGGACTGTTATTAGAATGAATAGTAATATGACTACTGTCACATTTTGCAAATGTGTAAAGAAGAAAGAGGGCTAAGTGAATCAAAGGG
AGACAGCCCCACTCACCCTGTTCTGCCCCAGAGGACTGAGCGATCCCCACCATCTGGGAAGCTGCCTCACCAGGAGGTCCAGCTGGGGCTACAGGACTGG
CTACTTTGCTACAATGGCCCATCTTTCCTGAGCCCAGTGGAGGGTCCCAGGGGGGCAGAAGTCATTGATAGGGGCCAGTAGGGTTGTAGAGCCACTGTCT
GAACTTCTGTGGAGGTCTGGTGCAGGGGAGGTGTGAACAGATAGGAGGCTAGGTGAGGATGCAGCAGAGGAAGGGGGCAGGAGTGCCCCAGGAGGGGGCG
GTACCGAGGCAGGGATTCAGCTGGGTCTGAGAGGGAGGGAAGGCTGAGGGGGATGGCCCTTTGGAGTGGGCAGGGACATGACCACAGAAAGCCTGGGAAG
TGGAAACAGACAGGAGCCTGCTGGAGCTCTTGGAGCTTTGTGTGGGGCACTAGGGGAAGGAGGCAGGCACTCCACCCTGACCTGCCCCTACCTCTGGATG
AGGGTCTTCTCTGCCTGTTGGATGATGTGCTGCCCCTGCTCTTGGAGGAAGAGGCCTCCCAGCCACCCCCTCCTGCCAGTCGCCTTGCCCTGCTGACCCA
GACAGATCTGCTATCCCTACAGCATCCAGGAAGGCCACAGGGAGGGGGCCCAGGTGGCATGGGTTTCTGAGGCCTGGGATCTGCTCTCAGTCCCGCTCTC
GCCAACCCCCTTTGCCCCTCTGTGACAGTGTGTGTACATATGCGTGCATGTGTGTACCTGTGTGGTGTGGCTCACAAGGTCACCCCTGGGGGTGGGATAA
TAAGAGGTAAAGTGTGACCCCCTCTCTTCCGTACATTCATTTTTTCAAGCTTGTGAATATTCACATTGTTAATATAATCTTCTGCAGTCCCATGACTTCG
AATAGCACTTAGATGCCGATGACCCCCCCACCAAAGAAACCCCACTGTCTCCCACAGACTCCAGGTCTGTATCTCCAACTGCCTACTCAATACAACACGT
CCACCTGATACAATGAACTTAATGTGTCTAAACCCCAATTCCACATTTCCAGTCCTCCTACCGGCTCCTCCCCCAGTCTTCTCTACTTTACTATATGACA
ACTCCATTTTTCCCAATACTGGTTTTAAAAACCCTGGAGTAGTCCTTGACTCCAGTCTCTCTCACCTTTCAACCAATTCAGCAGCAAATACTGACAGCTC
TACCTTTGCTCATCAAAGCATATCCCCAAATCACCCTATTCCAGACTGCACACTGTCATCTCTCCCTTCAATTACAGCAGTAGCCTCCTAACTAGTTTTC
TTGATTCCACTCCTGCCCATCAGCAGTGAAATTACCCCAGAGCAGTTAAAATGATCTTTTAGGATGGGCACAGTGGCTCACGCCTGTAATACCAGCACTT
TGGGAGGTGGAGGCTGGCTGATCCCCTTAGGTTGGGAGTTCAAGACCATCCTCACCAACAGGGAGAAACCCCATCTCTACTAAAAATACAAAAATTAGCC
GGATGTGGTGGGACGTGTCTGTAATCCCAACTACTTGGGAGGCTGAGACAGGAGAACTGCTTGAACCCGGGAGGTGGAGGTTGCAGTGAGCCAGAATCGT
GCCACAGCACTCCAGCCTGGGCAAGAAGCGCGAAACTCCGTCTCAAAATAAAATAATCTTCTAAAAATGACAGGGCCAGGTGGGGTGGCACTTTTTTATA
ATCCGAGCACTTTGGGAGGCTGAGGTGGGCAGATCGCTTGACATCAGGGGTTTGAGACCAGCCTGGCCAACATGGTGAAACTCCGTCTCTACTAAAAATA
CTAAAAATTAGCTGGGCGTGGTGGCGGGTGCCTGTAATCCCAGCTACTCGGGAGGCTGATGCAGGAGAATCGCCTGAACCAGGCAGATGCAGGATGCAGT
GAGCCAAGATTAAGACACTGCACTCCAACCTAAGCAATACTTTGTCTCAAAAATAAAAAAAAGCCTGGGAAACAAAGTGAGACCCCGTCTCTACAAAAAA
GTCAAAAAATTAGCTGGGTATGGTGGCAGTGATGGCACACACCTGTAGTCCCGGCTACTTGGGAAGCTTTTTAATATTTTTTGCAGAGACCGGGTCTCAC
TCTGTTACCTGGCCTGGTCTTGAACTCCTGGGCTCCAACAATCCCCTTTCCTGGGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACCGTGCCCAGC
CTCAAAGCATATTTTAAAGGATAGAAATAAACAGCCATATGAAGAGATACAGACAGGGCGGTCTGGAAGGGTCCAGAGCAGGAGCTTCTATCTCCATAGA
GTTGGGGTTACGTCACCCTCTGGGCACATTCTGTCAGCCTCCACACGTTCAGCCCTCAGAAGCTCCCGAACCCTGTCCTTTGGGCCTTTTATGGAGAACT
CCATTGGCTGTCCATGACTGAAGCATGGACAACTGTGATAATGTGATTGGGCAAAAAGGGTCTGATCTAAGCCCAGCAAGGCCAGTCCAGATTCTTTGGG
CCTTTGTGCAGCATTCCTTTCTCCAGGGTATGGGGCAAGGACCCACTCTGGAATGAGGATCCTACAACCCACAATCAGATTAGAGTCCTGCCTTGGGCAG
CTGAAAAGAGGACAGGAGAAGGTCAGAGAGACGAAAGGCTGTTTTTTGAGGCCTGAGGCACCCCAACATGACAACGTAAGACTGTAACCATGGTCATGTG
AGTTATGAGCTAGGAACCCTGGACGAAACCAGCACATATACAATCATCTCCCACCTCCCAACGCCTTTACTTTCACAGCCTCTGCAGCAAACTGCGGTCA
CTATAATCGCTCCTGTGGCACAGAGGCATACCCAGGGGAATCTGCCCAGGGGGCCACTCTGTGCCCACGTGGGAACCCACACCTGCTTGTAAAGCCTCCC
CTCCCTCTGACCAGCAACCAGGACAGTTTGTTGTTCCAAGCAGTGGGCTCATGTCTGTTTTGGCTCAGAACAGGGTGGGGAGAGCGGGCCAGGGACCCGC
AGGAAGGCTTATCCTTGAGATTGCGTGGGAGACACAACAAGGGGTGGGGGCCCGCAGGCGGGGCGGGGCGAAGCAGGTGATATCAAGCCCAGAGCCCCAG
CCTCTCCCCACAGTCTCACCATGGCCCGCACCGTGGTGCTCATCACCGGCTGTTCCTCGGGCATCGGCCTGCACTTGGCCGTACGTCTGGCTTCAGATCC
ATCCCAGAGCTTCAAAGTGTATGCCACGTTGAGGGACCTGAAAACACAGGGCCGGCTGTGGGAGGCGGCCCGGGCCCTGGCATGCCCTCCGGGATCCCTG
GAGACGTTGCAGCTGGACGTAAGGGACTCAAAATCCGTGGCCGCTGCCCGGGAACGCGTGACTGAGGGCCGCGTGGACGTGCTGGGTGAGCCTCCTGGAA
GCATATGGGCTCCTAGGAGCCTTCTCCGCCCTGCGTTGAAACCAACATGTTCCCAGGCCCAGGGAGCACGAGGGGACAGGCCGTGCTGAGGGTGATGCTG
AGGCGGGCTGGTCGGGCCTCTTGTCTCCGCAGTGTGTAACGCAGGCCTGGGCCTGCTGGGGCCGCTGGAGGCGCTGGGGGAGGACGCCGTGGCCTCTGTG
CTGGACGTGAATGTAGTAGGGACTGTGCGGATGCTGCAGGCCTTCCTGCCAGACATGAAGAGGCGCGGTTCGGGACGCGTGTTGGTGACCGGGAGCGTGG
GAGGATTGATGGGTGAGTGGTAGGGAGTGGCCTCGGCAGCTCCAGATTCTTTGTGTGCGGAGCTGAGCCTTGAAGGCAGGTTCCGCGGGGGGGGTGGAGT
GGGGTGCCGTCAGCTTGGAGGGGCACCGTCTGCCCGGGGATGACCCCCTGGCCGCTGCGCCTCAGGAACCTCGTCTCCCCACCTAAGGGCTGCCTTTCAA
TGACGTTTATTGCGCCAGCAAGTTCGCGCTCGAAGGCTTATGCGAGAGTCTGGCGGTTCTGCTGCTGCCCTTTGGGGTCCAGTGAGTCAACACCCCCGTT
CCCCGAACCCTCTTAACTCTGACCTAGAGACGCCGAGCACCCTGTCCTGCGGGAGCCGCTCTGGGGCGATCTCCCTGGCCCTCTCTGCCCGGCTCACATT
AGCTGTGTGCCAGGCACTTGGGCTGGGCGCATGGCACGCATTGTGCCACCTGCTGACCTAGCTGCTGAAGTGTTGGTATTGTTATGGGGAAGCTCCAGCC
CAGAGAGGTTAGGTGACTGGCCCAAGGTCACACAGCGGCCAGGGACCCCGCTAGATTCGAATCCTGACACCAGGGCTCCCTTGTAGCCTCAGATGGATTT
GGGAGGGCTGCTCCGGCAGGAACCCGCGTTTCAAATGTTCTGGTTATCCCCAGCGCCCTTTCCGCCTCACTTCCCAGCGCAGCGGTGCTGCTCGCGGTCG
GGGGCCGGGACGTGGTTGGGGCTGGGACTGGGGTTGGGGCTGGGACTGGGGCCTGGCTGGCGTCCGCCCCCTCCCACTCGTTGCTCTCCGGCCAGCAGCT
TGAGCCTGATCGAGTGCGGCCCAGTGCACACCGCCTTCATGGAGAAGGTGTTGGGCAGCCCAGAGGAGGTGCTGGACCGCACGGACATCCACACCTTCCA
CCGCTTCTACCAATACCTCGCCCACAGCAAGCAAGTCTTTCGCGAGGCGGCGCAGAACCCTGAGGAGGTGGCGGAGGTGAGCGCCGGGCTGGACTCCAGG
AGTGGGGGCGGTGCGTCCTCCGGCGCGCAGCGGTGGCCACAGCTCTCCTCCCGCCGCCGCAGGTCTTCCTCACCGCTTTGCGCGCCCCGAAGCCGACCCT
GCGCTACTTCACCACCGAGCGCTTCCTGCCCCTGCTGCGGATGCGCCTGGACGACCCCAGCGGCTCCAACTACGTCACCGCCATGCACCGGGAAGTGTTC
GGCGACGTTCCGGCAAAGGCCGAGGCTGGGGCCGAGGCTGGGGGCGGGGCCGGGCCTGGGGCAGAGGACGAGGCCGGGCGCGGTGCGGTGGGGGACCCTG
AGCTCGGCGATCCTCCGGCCGCCCCGCAGTAAAGGCTTCCTCAGCCGCTGTCTCCCGCGCCCTTCTTTGTCCCCTGGGTCTGTGTGGTCCCTGGGGATGG
GGCGGCGGTAGCAGCTGTGGGTGGCTAATTAAGATAGATCGCGTTAGCCAGTTTTACCAGCGCAGCTAGGCGCGATGGCTGTCGCCTGTAATGCCAGCGC
TTTGGGAGGCGGAGGCAGGAGGATCGCTCAAGCCCCGGAGTTGGAGACCAGCCAGAGCAACACAGTGAGACCCCCATCTCTACAAAAATAAAGAAAATTT
- HSD17B1.F4 (forward primer)
- HSD17B1.R4 (reverse primer)
- Intron retention
Frame 0
S*VGLGLG*SQVVTCTPLAMRPQASSLLSLSAF
LSFPFSFLFCFSFVLRRSFTLVAQPGVQ*RGLSS
PQPLPPGFKCS*DCRHVPPRPANFVFLVETRFL
HVGQTGLGLPTSGDPPALASQSAGITGMTHPIR
PSESFLSVKWV**YLSNRF**WWR*CLKVLV*RF
NTQQGLLLE*IVI*LLSHFANV*RRKRAK*IKG
RQPHSPCSAPED*AIPTIWEAASPGGPAGATGL
ATLLQWPIFPEPSGGSQGGRSH**GPVGL*SHCL
NFCGGLVQGRCEQIGG*VRMQQRKGAGVPQEGA
VPRQGFSWV*EGGKAEGDGPLEWAGT*PQKAWE
VETDRSLLELLELCVGH*GKEAGTPP*PAPTSG*
GSSLPVG*CAAPALGGRGLPATPSCQSPCPADP
DRSAIPTASRKATGRGPRWHGFLRPGICSQSRS
RQPPLPLCDSVCTYACMCVPVWCGSQGHPWGWDN
KR*SVTPSLPYIHFFKLVNIHIVNIIFCSPMTS
NST*MPMTPPPKKPHCLPQTPGLYLQLPTQYNT
ST*YNELNVSKPQFHISSPPTGSSPSLLYFTI*Q
LHFSQYWF*KPWSSP*LQSLSPFNQFSSKY*QL
YLCSSKHIPKSPYSRLHTVISPFNYSSSLLTSF
LDSTPAHQQ*NYPRAVKMIF*DGHSGSRL*YQHF
GRWRLADPLRLGVQDHPHQQGETPSLLKIQKLA
GCGGTCL*SQLLGRLRQENCLNPGGGGCSEPES
CHSTPAWARSAKLRLKIK*SSKNDRARWGGTFL*
SEHFGRLRWADRLTSGV*DQPGQHGETPSLLKI
LKISWAWWRVPVIPATREADAGESPEPGRCRMQ
*AKIKTLHSNLSNTLSQK*KKAWETK*DPVSTKK
SKN*LGMVAVMAHTCSPGYLGSFLIFFAETGSH
SVTWPGLELLGSNNPLSWASQSAGITGMSHRAQ
PQSIF*RIEINSHMKRYRQGGLEGSRAGASISIE
LGLRHPLGTFCQPPHVQPSEAPEPCPLGLLWRT
PLAVHD*SMDNCDNVIGQKGSDLSPARPVQILW
AFVQHSFLQGMGQGPTLE*GSYNPQSD*SPALGS
*KEDRRRSERRKAVF*GLRHPNMTT*DCNHGHV
SYELGTLDETSTYTIISHLPTPLLSQPLQQTAV
TIIAPVAQRHTQGNLPRGPLCAHVGTHTCL*SLP
SL*PATRTVCCSKQWAHVCFGSEQGGESGPGTR
RKAYP*DCVGDTTRGGGPQAGRGEAGDIKPRAP
ASPHSLTMARTVVLITGCSSGIGLHLAVRLASDP
SQSFKVYATLRDLKTQGRLWEAARALACPPGSL
ETLQLDVRDSKSVAAARERVTEGRVDVLGEPPG
SIWAPRSLLRPALKPTCSQAQGARGDRPC*G*C*
GGLVGPLVSAVCNAGLGLLGPLEALGEDAVASV
LDVNVVGTVRMLQAFLPDMKRRGSGRVLVTGSV
GGLMGEW*GVASAAPDSLCAELSLEGRFRGGGGV
GCRQLGGAPSARG*PPGRCASGTSSPHLRAAFQ
*RLLRQQVRARRLMRESGGSAAALWGPVSQHPR
SPNPLNSDLETPSTLSCGSRSGAISLALSARLTL
AVCQALGLGAWHALCHLLT*LLKCWYCYGEAPA
QRG*VTGPRSHSGQGPR*IRILTPGLPCSLRWI
WEGCSGRNPRFKCSGYPQRPFRLTSQRSGAARGR
GPGRGWGWDWGWGWDWGLAGVRPLPLVALRPAA
*A*SSAAQCTPPSWRRCWAAQRRCWTARTSTPS
TASTNTSPTASKSFARRRRTLRRWRR*APGWTPG
VGAVRPPARSGGHSSPPAAAGLPHRFARPEADP
ALLHHRALPAPAADAPGRPQRLQLRHRHAPGSV
RRRSGKGRGWGRGWGRGRAWGRGRGRARCGGGP*
ARRSSGRPAVKASSAAVSRALLCPLGLCGPWGW
GGGSSCGWLIKIDRVSQFYQRS*ARWLSPVMPA
LWEAEAGGSLKPRSWRPARATQ*DPHLYKNKEN
Frame 1
VRWDLGWGRVRL*PALH*P*DLRQVPCFL*VLS
FPFPFLFFFVFLLF*DGVSLLLPSLECNSAVSV
HHNLCLPGSSVARIAGMCHHAQLILYF**RRGFS
MLVRLVLDSRPQVIRPPWPLRVLGLQA*PTPSG
PLSLSYL*NGYNNTYLIGFSNGGDNA*KC*YRG
LIPSKDCY*NE**YDYCHILQMCKEERGLSESKG
DSPTHPVLPQRTERSPPSGKLPHQEVQLGLQDW
LLCYNGPSFLSPVEGPRGAEVIDRGQ*GCRATV
*TSVEVWCRGGVNR*EAR*GCSRGRGQECPRRGR
YRGRDSAGSEREGRLRGMALWSGQGHDHRKPGK
WKQTGACWSSWSFVWGTRGRRQALHPDLPLPLD
EGLLCLLDDVLPLLLEEEASQPPPPASRLALLTQ
TDLLSLQHPGRPQGGGPGGMGF*GLGSALSPAL
ANPLCPSVTVCVHMRACVYLCGVAHKVTPGGGI
IRGKV*PPLFRTFIFSSL*IFTLLI*SSAVP*LR
IALRCR*PPHQRNPTVSHRLQVCISNCLLNTTR
PPDTMNLMCLNPNSTFPVLLPAPPPVFSTLLYD
NSIFPNTGFKNPGVVLDSSLSHLSTNSAANTDSS
TFAHQSISPNHPIPDCTLSSLPSITAVAS*LVF
LIPLLPISSEITPEQLK*SFRMGTVAHACNTST
LGGGGWLIPLGWEFKTILTNREKPHLY*KYKN*P
DVVGRVCNPNYLGG*DRRTA*TREVEVAVSQNR
ATALQPGQEARNSVSK*NNLLKMTGPGGVALFY
NPSTLGG*GGQIA*HQGFETSLANMVKLRLY*KY
*KLAGRGGGCL*SQLLGRLMQENRLNQADAGCS
EPRLRHCTPT*AILCLKNKKKPGKQSETPSLQK
SQKISWVWWQ*WHTPVVPATWEAF*YFLQRPGLT
LLPGLVLNSWAPTIPFPGPPKVLGLQA*ATVPS
LKAYFKG*K*TAI*RDTDRAVWKGPEQELLSP*
SWGYVTLWAHSVSLHTFSPQKLPNPVLWAFYGEL
HWLSMTEAWTTVIM*LGKKGLI*AQQGQSRFFG
PLCSIPFSRVWGKDPLWNEDPTTHNQIRVLPWA
AEKRTGEGQRDERLFFEA*GTPT*QRKTVTMVM*
VMS*EPWTKPAHIQSSPTSQRLYFHSLCSKLRS
L*SLLWHRGIPRGICPGGHSVPTWEPTPACKAS
PPSDQQPGQFVVPSSGLMSVLAQNRVGRAGQGPA
GRLILEIAWETQQGVGARRRGGAKQVISSPEPQ
PLPTVSPWPAPWCSSPAVPRASACTWPYVWLQI
HPRASKCMPR*GT*KHRAGCGRRPGPWHALRDPW
RRCSWT*GTQNPWPLPGNA*LRAAWTCWVSLLE
AYGLLGAFSALR*NQHVPRPREHEGTGRAEGDA
EAGWSGLLSPQCVTQAWACWGRWRRWGRTPWPLC
WT*M**GLCGCCRPSCQT*RGAVRDACW*PGAW
ED*WVSGREWPRQLQILCVRS*ALKAGSAGGVE
WGAVSLEGHRLPGDDPLAAAPQEPRLPT*GLPFN
DVYCASKFALEGLCESLAVLLLPFGVQ*VNTPV
PRTLLTLT*RRRAPCPAGAALGRSPWPSLPGSH
*LCARHLGWAHGTHCATC*PSC*SVGIVMGKLQP
REVR*LAQGHTAARDPARFES*HQGSLVASDGF
GRAAPAGTRVSNVLVIPSALSASLPSAAVLLAV
GGRDVVGAGTGVGAGTGAWLASAPSHSLLSGQQL
EPDRVRPSAHRLHGEGVGQPRGGAGPHGHPHLP
PLLPIPRPQQASLSRGGAEP*GGGGGERRAGLQ
EWGRCVLRRAAVATALLPPPQVFLTALRAPKPTL
RYFTTERFLPLLRMRLDDPSGSNYVTAMHREVF
GDVPAKAEAGAEAGGGAGPGAEDEAGRGAVGDP
ELGDPPAAPQ*RLPQPLSPAPFFVPWVCVVPGDG
AAVAAVGG*LR*IALASFTSAARRDGCRL*CQR
FGRRRQEDRSSPGVGDQPEQHSETPISTKIKKI
Frame 2
LGGTWVGVESGCDLHSISHETSGKFLAFSECFP
FLSLFFSFLFFFCFETEFHSCCPAWSAIARSQF
TTTSASRVQV*LGLQACATTPS*FCIFSRDEVS
PCWSDWSWTPDLR*SARPGLSECWDYRHDPPHPA
L*VFLICKMGIIIPI*SVLVMVEIMPESASIEV
*YPARTVIRMNSNMTTVTFCKCVKKKEG*VNQR
ETAPLTLFCPRGLSDPHHLGSCLTRRSSWGYRTG
YFATMAHLS*AQWRVPGGQKSLIGASRVVEPLS
ELLWRSGAGEV*TDRRLGEDAAEEGGRSAPGGG
GTEAGIQLGLRGREG*GGWPFGVGRDMTTESLGS
GNRQEPAGALGALCGALGEGGRHSTLTCPYLWM
RVFSACWMMCCPCSWRKRPPSHPLLPVALPC*P
RQICYPYSIQEGHREGAQVAWVSEAWDLLSVPLS
PTPFAPL*QCVYICVHVCTCVVWLTRSPLGVG*
*EVKCDPLSSVHSFFQACEYSHC*YNLLQSHDF
E*HLDADDPPTKETPLSPTDSRSVSPTAYSIQHV
HLIQ*T*CV*TPIPHFQSSYRLLPQSSLLYYMT
TPFFPILVLKTLE*SLTPVSLTFQPIQQQILTA
LPLLIKAYPQITLFQTAHCHLSLQLQQ*PPN*FS
*FHSCPSAVKLPQSS*NDLLGWAQWLTPVIPAL
WEVEAG*SP*VGSSRPSSPTGRNPISTKNTKIS
RMWWDVSVIPTTWEAETGELLEPGRWRLQ*ARIV
PQHSSLGKKRETPSQNKIIF*K*QGQVGWHFFI
IRALWEAEVGRSLDIRGLRPAWPTW*NSVSTKN
TKN*LGVVAGACNPSYSGG*CRRIA*TRQMQDAV
SQD*DTALQPKQYFVSKIKKSLGNKVRPRLYKK
VKKLAGYGGSDGTHL*SRLLGKLFNIFCRDRVS
LCYLAWS*TPGLQQSPFLGLPKCWDYRHEPPCPA
SKHILKDRNKQPYEEIQTGRSGRVQSRSFYLHR
VGVTSPSGHILSASTRSALRSSRTLSFGPFMEN
SIGCP*LKHGQL**CDWAKRV*SKPSKASPDSLG
LCAAFLSPGYGARTHSGMRILQPTIRLESCLGQ
LKRGQEKVRETKGCFLRPEAPQHDNVRL*PWSC
EL*ARNPGRNQHIYNHLPPPNAFTFTASAANCGH
YNRSCGTEAYPGESAQGATLCPRGNPHLLVKPP
LPLTSNQDSLLFQAVGSCLFWLRTGWGERARDP
QEGLSLRLRGRHNKGWGPAGGAGRSR*YQAQSPS
LSPQSHHGPHRGAHHRLFLGHRPALGRTSGFRS
IPELQSVCHVEGPENTGPAVGGGPGPGMPSGIP
GDVAAGRKGLKIRGRCPGTRD*GPRGRAG*ASWK
HMGS*EPSPPCVETNMFPGPGSTRGQAVLRVML
RRAGRASCLRSV*RRPGPAGAAGGAGGGRRGLC
AGRECSRDCADAAGLPARHEEARFGTRVGDRERG
RIDG*VVGSGLGSSRFFVCGAEP*RQVPRGGWS
GVPSAWRGTVCPGMTPWPLRLRNLVSPPKGCLS
MTFIAPASSRSKAYARVWRFCCCPLGSSESTPPF
PEPS*L*PRDAEHPVLREPLWGDLPGPLCPAHI
SCVPGTWAGRMARIVPPADLAAEVLVLLWGSSS
PERLGDWPKVTQRPGTPLDSNPDTRAPL*PQMDL
GGLLRQEPAFQMFWLSPAPFPPHFPAQRCCSRS
GAGTWLGLGLGLGLGLGPGWRPPPPTRCSPASS
LSLIECGPVHTAFMEKVLGSPEEVLDRTDIHTFH
RFYQYLAHSKQVFREAAQNPEEVAEVSAGLDSR
SGGGASSGAQRWPQLSSRRRRSSSPLCAPRSRP
CATSPPSASCPCCGCAWTTPAAPTTSPPCTGKCS
ATFRQRPRLGPRLGAGPGLGQRTRPGAVRWGTL
SSAILRPPRSKGFLSRCLPRPSLSPGSVWSLGM
GRR*QLWVAN*DRSR*PVLPAQLGAMAVACNASA
LGGGGRRIAQAPELETSQSNTVRPPSLQK*RKF
Sequence HSD17B1.eAug05
AGTTAGGTGGGACTTGGGTTGGGGTAGAGTCAGGTTGTGACCTGCACTCCATTAGCCATGAGACCTCAGGCAAGTTCCTTGCTTTCTCTGAGTGCTTTCC
TTTCCTTTCCCTTTTCTTTTCTTTTTTGTTTTTCTTTTGTTTTGAGACGGAGTTTCACTCTTGTTGCCCAGCCTGGAGTGCAATAGCGCGGTCTCAGTTC
ACCACAACCTCTGCCTCCCGGGTTCAAGTGTAGCTAGGATTGCAGGCATGTGCCACCACGCCCAGCTAATTTTGTATTTTTAGTAGAGACGAGGTTTCTC
CATGTTGGTCAGACTGGTCTTGGACTCCCGACCTCAGGTGATCCGCCCGCCCTGGCCTCTCAGAGTGCTGGGATTACAGGCATGACCCACCCCATCCGGC
CCTCTGAGTCTTTCTTATCTGTAAAATGGGTATAATAATACCTATCTAATCGGTTTTAGTAATGGTGGAGATAATGCCTGAAAGTGCTAGTATAGAGGTT
TAATACCCAGCAAGGACTGTTATTAGAATGAATAGTAATATGACTACTGTCACATTTTGCAAATGTGTAAAGAAGAAAGAGGGCTAAGTGAATCAAAGGG
AGACAGCCCCACTCACCCTGTTCTGCCCCAGAGGACTGAGCGATCCCCACCATCTGGGAAGCTGCCTCACCAGGAGGTCCAGCTGGGGCTACAGGACTGG
CTACTTTGCTACAATGGCCCATCTTTCCTGAGCCCAGTGGAGGGTCCCAGGGGGGCAGAAGTCATTGATAGGGGCCAGTAGGGTTGTAGAGCCACTGTCT
GAACTTCTGTGGAGGTCTGGTGCAGGGGAGGTGTGAACAGATAGGAGGCTAGGTGAGGATGCAGCAGAGGAAGGGGGCAGGAGTGCCCCAGGAGGGGGCG
GTACCGAGGCAGGGATTCAGCTGGGTCTGAGAGGGAGGGAAGGCTGAGGGGGATGGCCCTTTGGAGTGGGCAGGGACATGACCACAGAAAGCCTGGGAAG
TGGAAACAGACAGGAGCCTGCTGGAGCTCTTGGAGCTTTGTGTGGGGCACTAGGGGAAGGAGGCAGGCACTCCACCCTGACCTGCCCCTACCTCTGGATG
AGGGTCTTCTCTGCCTGTTGGATGATGTGCTGCCCCTGCTCTTGGAGGAAGAGGCCTCCCAGCCACCCCCTCCTGCCAGTCGCCTTGCCCTGCTGACCCA
GACAGATCTGCTATCCCTACAGCATCCAGGAAGGCCACAGGGAGGGGGCCCAGGTGGCATGGGTTTCTGAGGCCTGGGATCTGCTCTCAGTCCCGCTCTC
GCCAACCCCCTTTGCCCCTCTGTGACAGTGTGTGTACATATGCGTGCATGTGTGTACCTGTGTGGTGTGGCTCACAAGGTCACCCCTGGGGGTGGGATAA
TAAGAGGTAAAGTGTGACCCCCTCTCTTCCGTACATTCATTTTTTCAAGCTTGTGAATATTCACATTGTTAATATAATCTTCTGCAGTCCCATGACTTCG
AATAGCACTTAGATGCCGATGACCCCCCCACCAAAGAAACCCCACTGTCTCCCACAGACTCCAGGTCTGTATCTCCAACTGCCTACTCAATACAACACGT
CCACCTGATACAATGAACTTAATGTGTCTAAACCCCAATTCCACATTTCCAGTCCTCCTACCGGCTCCTCCCCCAGTCTTCTCTACTTTACTATATGACA
ACTCCATTTTTCCCAATACTGGTTTTAAAAACCCTGGAGTAGTCCTTGACTCCAGTCTCTCTCACCTTTCAACCAATTCAGCAGCAAATACTGACAGCTC
TACCTTTGCTCATCAAAGCATATCCCCAAATCACCCTATTCCAGACTGCACACTGTCATCTCTCCCTTCAATTACAGCAGTAGCCTCCTAACTAGTTTTC
TTGATTCCACTCCTGCCCATCAGCAGTGAAATTACCCCAGAGCAGTTAAAATGATCTTTTAGGATGGGCACAGTGGCTCACGCCTGTAATACCAGCACTT
TGGGAGGTGGAGGCTGGCTGATCCCCTTAGGTTGGGAGTTCAAGACCATCCTCACCAACAGGGAGAAACCCCATCTCTACTAAAAATACAAAAATTAGCC
GGATGTGGTGGGACGTGTCTGTAATCCCAACTACTTGGGAGGCTGAGACAGGAGAACTGCTTGAACCCGGGAGGTGGAGGTTGCAGTGAGCCAGAATCGT
GCCACAGCACTCCAGCCTGGGCAAGAAGCGCGAAACTCCGTCTCAAAATAAAATAATCTTCTAAAAATGACAGGGCCAGGTGGGGTGGCACTTTTTTATA
ATCCGAGCACTTTGGGAGGCTGAGGTGGGCAGATCGCTTGACATCAGGGGTTTGAGACCAGCCTGGCCAACATGGTGAAACTCCGTCTCTACTAAAAATA
CTAAAAATTAGCTGGGCGTGGTGGCGGGTGCCTGTAATCCCAGCTACTCGGGAGGCTGATGCAGGAGAATCGCCTGAACCAGGCAGATGCAGGATGCAGT
GAGCCAAGATTAAGACACTGCACTCCAACCTAAGCAATACTTTGTCTCAAAAATAAAAAAAAGCCTGGGAAACAAAGTGAGACCCCGTCTCTACAAAAAA
GTCAAAAAATTAGCTGGGTATGGTGGCAGTGATGGCACACACCTGTAGTCCCGGCTACTTGGGAAGCTTTTTAATATTTTTTGCAGAGACCGGGTCTCAC
TCTGTTACCTGGCCTGGTCTTGAACTCCTGGGCTCCAACAATCCCCTTTCCTGGGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACCGTGCCCAGC
CTCAAAGCATATTTTAAAGGATAGAAATAAACAGCCATATGAAGAGATACAGACAGGGCGGTCTGGAAGGGTCCAGAGCAGGAGCTTCTATCTCCATAGA
GTTGGGGTTACGTCACCCTCTGGGCACATTCTGTCAGCCTCCACACGTTCAGCCCTCAGAAGCTCCCGAACCCTGTCCTTTGGGCCTTTTATGGAGAACT
CCATTGGCTGTCCATGACTGAAGCATGGACAACTGTGATAATGTGATTGGGCAAAAAGGGTCTGATCTAAGCCCAGCAAGGCCAGTCCAGATTCTTTGGG
CCTTTGTGCAGCATTCCTTTCTCCAGGGTATGGGGCAAGGACCCACTCTGGAATGAGGATCCTACAACCCACAATCAGATTAGAGTCCTGCCTTGGGCAG
CTGAAAAGAGGACAGGAGAAGGTCAGAGAGACGAAAGGCTGTTTTTTGAGGCCTGAGGCACCCCAACATGACAACGTAAGACTGTAACCATGGTCATGTG
AGTTATGAGCTAGGAACCCTGGACGAAACCAGCACATATACAATCATCTCCCACCTCCCAACGCCTTTACTTTCACAGCCTCTGCAGCAAACTGCGGTCA
CTATAATCGCTCCTGTGGCACAGAGGCATACCCAGGGGAATCTGCCCAGGGGGCCACTCTGTGCCCACGTGGGAACCCACACCTGCTTGTAAAGCCTCCC
CTCCCTCTGACCAGCAACCAGGACAGTTTGTTGTTCCAAGCAGTGGGCTCATGTCTGTTTTGGCTCAGAACAGGGTGGGGAGAGCGGGCCAGGGACCCGC
AGGAAGGCTTATCCTTGAGATTGCGTGGGAGACACAACAAGGGGTGGGGGCCCGCAGGCGGGGCGGGGCGAAGCAGGTGATATCAAGCCCAGAGCCCCAG
CCTCTCCCCACAGTCTCACCATGGCCCGCACCGTGGTGCTCATCACCGGCTGTTCCTCGGGCATCGGCCTGCACTTGGCCGTACGTCTGGCTTCAGATCC
ATCCCAGAGCTTCAAAGTGTATGCCACGTTGAGGGACCTGAAAACACAGGGCCGGCTGTGGGAGGCGGCCCGGGCCCTGGCATGCCCTCCGGGATCCCTG
GAGACGTTGCAGCTGGACGTAAGGGACTCAAAATCCGTGGCCGCTGCCCGGGAACGCGTGACTGAGGGCCGCGTGGACGTGCTGGTGTGTAACGCAGGCC
TGGGCCTGCTGGGGCCGCTGGAGGCGCTGGGGGAGGACGCCGTGGCCTCTGTGCTGGACGTGAATGTAGTAGGGACTGTGCGGATGCTGCAGGCCTTCCT
GCCAGACATGAAGAGGCGCGGTTCGGGACGCGTGTTGGTGACCGGGAGCGTGGGAGGATTGATGGGTGAGTGGTAGGGAGTGGCCTCGGCAGCTCCAGAT
TCTTTGTGTGCGGAGCTGAGCCTTGAAGGCAGGTTCCGCGGGGGGGGTGGAGTGGGGTGCCGTCAGCTTGGAGGGGCACCGTCTGCCCGGGGATGACCCC
CTGGCCGCTGCGCCTCAGGAACCTCGTCTCCCCACCTAAGGGCTGCCTTTCAATGACGTTTATTGCGCCAGCAAGTTCGCGCTCGAAGGCTTATGCGAGA
GTCTGGCGGTTCTGCTGCTGCCCTTTGGGGTCCAGTGAGTCAACACCCCCGTTCCCCGAACCCTCTTAACTCTGACCTAGAGACGCCGAGCACCCTGTCC
TGCGGGAGCCGCTCTGGGGCGATCTCCCTGGCCCTCTCTGCCCGGCTCACATTAGCTGTGTGCCAGGCACTTGGGCTGGGCGCATGGCACGCATTGTGCC
ACCTGCTGACCTAGCTGCTGAAGTGTTGGTATTGTTATGGGGAAGCTCCAGCCCAGAGAGGTTAGGTGACTGGCCCAAGGTCACACAGCGGCCAGGGACC
CCGCTAGATTCGAATCCTGACACCAGGGCTCCCTTGTAGCCTCAGATGGATTTGGGAGGGCTGCTCCGGCAGGAACCCGCGTTTCAAATGTTCTGGTTAT
CCCCAGCGCCCTTTCCGCCTCACTTCCCAGCGCAGCGGTGCTGCTCGCGGTCGGGGGCCGGGACGTGGTTGGGGCTGGGACTGGGGTTGGGGCTGGGACT
GGGGCCTGGCTGGCGTCCGCCCCCTCCCACTCGTTGCTCTCCGGCCAGCAGCTTGAGCCTGATCGAGTGCGGCCCAGTGCACACCGCCTTCATGGAGAAG
GTGTTGGGCAGCCCAGAGGAGGTGCTGGACCGCACGGACATCCACACCTTCCACCGCTTCTACCAATACCTCGCCCACAGCAAGCAAGTCTTTCGCGAGG
CGGCGCAGAACCCTGAGGAGGTGGCGGAGGTGAGCGCCGGGCTGGACTCCAGGAGTGGGGGCGGTGCGTCCTCCGGCGCGCAGCGGTGGCCACAGCTCTC
CTCCCGCCGCCGCAGGTCTTCCTCACCGCTTTGCGCGCCCCGAAGCCGACCCTGCGCTACTTCACCACCGAGCGCTTCCTGCCCCTGCTGCGGATGCGCC
TGGACGACCCCAGCGGCTCCAACTACGTCACCGCCATGCACCGGGAAGTGTTCGGCGACGTTCCGGCAAAGGCCGAGGCTGGGGCCGAGGCTGGGGGCGG
GGCCGGGCCTGGGGCAGAGGACGAGGCCGGGCGCGGTGCGGTGGGGGACCCTGAGCTCGGCGATCCTCCGGCCGCCCCGCAGTAAAGGCTTCCTCAGCCG
CTGTCTCCCGCGCCCTTCTTTGTCCCCTGGGTCTGTGTGGTCCCTGGGGATGGGGCGGCGGTAGCAGCTGTGGGTGGCTAATTAAGATAGATCGCGTTAG
CCAGTTTTACCAGCGCAGCTAGGCGCGATGGCTGTCGCCTGTAATGCCAGCGCTTTGGGAGGCGGAGGCAGGAGGATCGCTCAAGCCCCGGAGTTGGAGA
CCAGCCAGAGCAACACAGTGAGACCCCCATCTCTACAAAAATAAAGAAAATTT
- HSD17B1.F4 (forward primer)
- HSD17B1.R4 (reverse primer)
- Intron retention
Frame 0
S*VGLGLG*SQVVTCTPLAMRPQASSLLSLSAF
LSFPFSFLFCFSFVLRRSFTLVAQPGVQ*RGLSS
PQPLPPGFKCS*DCRHVPPRPANFVFLVETRFL
HVGQTGLGLPTSGDPPALASQSAGITGMTHPIR
PSESFLSVKWV**YLSNRF**WWR*CLKVLV*RF
NTQQGLLLE*IVI*LLSHFANV*RRKRAK*IKG
RQPHSPCSAPED*AIPTIWEAASPGGPAGATGL
ATLLQWPIFPEPSGGSQGGRSH**GPVGL*SHCL
NFCGGLVQGRCEQIGG*VRMQQRKGAGVPQEGA
VPRQGFSWV*EGGKAEGDGPLEWAGT*PQKAWE
VETDRSLLELLELCVGH*GKEAGTPP*PAPTSG*
GSSLPVG*CAAPALGGRGLPATPSCQSPCPADP
DRSAIPTASRKATGRGPRWHGFLRPGICSQSRS
RQPPLPLCDSVCTYACMCVPVWCGSQGHPWGWDN
KR*SVTPSLPYIHFFKLVNIHIVNIIFCSPMTS
NST*MPMTPPPKKPHCLPQTPGLYLQLPTQYNT
ST*YNELNVSKPQFHISSPPTGSSPSLLYFTI*Q
LHFSQYWF*KPWSSP*LQSLSPFNQFSSKY*QL
YLCSSKHIPKSPYSRLHTVISPFNYSSSLLTSF
LDSTPAHQQ*NYPRAVKMIF*DGHSGSRL*YQHF
GRWRLADPLRLGVQDHPHQQGETPSLLKIQKLA
GCGGTCL*SQLLGRLRQENCLNPGGGGCSEPES
CHSTPAWARSAKLRLKIK*SSKNDRARWGGTFL*
SEHFGRLRWADRLTSGV*DQPGQHGETPSLLKI
LKISWAWWRVPVIPATREADAGESPEPGRCRMQ
*AKIKTLHSNLSNTLSQK*KKAWETK*DPVSTKK
SKN*LGMVAVMAHTCSPGYLGSFLIFFAETGSH
SVTWPGLELLGSNNPLSWASQSAGITGMSHRAQ
PQSIF*RIEINSHMKRYRQGGLEGSRAGASISIE
LGLRHPLGTFCQPPHVQPSEAPEPCPLGLLWRT
PLAVHD*SMDNCDNVIGQKGSDLSPARPVQILW
AFVQHSFLQGMGQGPTLE*GSYNPQSD*SPALGS
*KEDRRRSERRKAVF*GLRHPNMTT*DCNHGHV
SYELGTLDETSTYTIISHLPTPLLSQPLQQTAV
TIIAPVAQRHTQGNLPRGPLCAHVGTHTCL*SLP
SL*PATRTVCCSKQWAHVCFGSEQGGESGPGTR
RKAYP*DCVGDTTRGGGPQAGRGEAGDIKPRAP
ASPHSLTMARTVVLITGCSSGIGLHLAVRLASDP
SQSFKVYATLRDLKTQGRLWEAARALACPPGSL
ETLQLDVRDSKSVAAARERVTEGRVDVLVCNAG
LGLLGPLEALGEDAVASVLDVNVVGTVRMLQAFL
PDMKRRGSGRVLVTGSVGGLMGEW*GVASAAPD
SLCAELSLEGRFRGGGGVGCRQLGGAPSARG*P
PGRCASGTSSPHLRAAFQ*RLLRQQVRARRLMRE
SGGSAAALWGPVSQHPRSPNPLNSDLETPSTLS
CGSRSGAISLALSARLTLAVCQALGLGAWHALC
HLLT*LLKCWYCYGEAPAQRG*VTGPRSHSGQGP
R*IRILTPGLPCSLRWIWEGCSGRNPRFKCSGY
PQRPFRLTSQRSGAARGRGPGRGWGWDWGWGWD
WGLAGVRPLPLVALRPAA*A*SSAAQCTPPSWRR
CWAAQRRCWTARTSTPSTASTNTSPTASKSFAR
RRRTLRRWRR*APGWTPGVGAVRPPARSGGHSS
PPAAAGLPHRFARPEADPALLHHRALPAPAADAP
GRPQRLQLRHRHAPGSVRRRSGKGRGWGRGWGR
GRAWGRGRGRARCGGGP*ARRSSGRPAVKASSA
AVSRALLCPLGLCGPWGWGGGSSCGWLIKIDRVS
QFYQRS*ARWLSPVMPALWEAEAGGSLKPRSWR
PARATQ*DPHLYKNKEN
Frame 1
VRWDLGWGRVRL*PALH*P*DLRQVPCFL*VLS
FPFPFLFFFVFLLF*DGVSLLLPSLECNSAVSV
HHNLCLPGSSVARIAGMCHHAQLILYF**RRGFS
MLVRLVLDSRPQVIRPPWPLRVLGLQA*PTPSG
PLSLSYL*NGYNNTYLIGFSNGGDNA*KC*YRG
LIPSKDCY*NE**YDYCHILQMCKEERGLSESKG
DSPTHPVLPQRTERSPPSGKLPHQEVQLGLQDW
LLCYNGPSFLSPVEGPRGAEVIDRGQ*GCRATV
*TSVEVWCRGGVNR*EAR*GCSRGRGQECPRRGR
YRGRDSAGSEREGRLRGMALWSGQGHDHRKPGK
WKQTGACWSSWSFVWGTRGRRQALHPDLPLPLD
EGLLCLLDDVLPLLLEEEASQPPPPASRLALLTQ
TDLLSLQHPGRPQGGGPGGMGF*GLGSALSPAL
ANPLCPSVTVCVHMRACVYLCGVAHKVTPGGGI
IRGKV*PPLFRTFIFSSL*IFTLLI*SSAVP*LR
IALRCR*PPHQRNPTVSHRLQVCISNCLLNTTR
PPDTMNLMCLNPNSTFPVLLPAPPPVFSTLLYD
NSIFPNTGFKNPGVVLDSSLSHLSTNSAANTDSS
TFAHQSISPNHPIPDCTLSSLPSITAVAS*LVF
LIPLLPISSEITPEQLK*SFRMGTVAHACNTST
LGGGGWLIPLGWEFKTILTNREKPHLY*KYKN*P
DVVGRVCNPNYLGG*DRRTA*TREVEVAVSQNR
ATALQPGQEARNSVSK*NNLLKMTGPGGVALFY
NPSTLGG*GGQIA*HQGFETSLANMVKLRLY*KY
*KLAGRGGGCL*SQLLGRLMQENRLNQADAGCS
EPRLRHCTPT*AILCLKNKKKPGKQSETPSLQK
SQKISWVWWQ*WHTPVVPATWEAF*YFLQRPGLT
LLPGLVLNSWAPTIPFPGPPKVLGLQA*ATVPS
LKAYFKG*K*TAI*RDTDRAVWKGPEQELLSP*
SWGYVTLWAHSVSLHTFSPQKLPNPVLWAFYGEL
HWLSMTEAWTTVIM*LGKKGLI*AQQGQSRFFG
PLCSIPFSRVWGKDPLWNEDPTTHNQIRVLPWA
AEKRTGEGQRDERLFFEA*GTPT*QRKTVTMVM*
VMS*EPWTKPAHIQSSPTSQRLYFHSLCSKLRS
L*SLLWHRGIPRGICPGGHSVPTWEPTPACKAS
PPSDQQPGQFVVPSSGLMSVLAQNRVGRAGQGPA
GRLILEIAWETQQGVGARRRGGAKQVISSPEPQ
PLPTVSPWPAPWCSSPAVPRASACTWPYVWLQI
HPRASKCMPR*GT*KHRAGCGRRPGPWHALRDPW
RRCSWT*GTQNPWPLPGNA*LRAAWTCWCVTQA
WACWGRWRRWGRTPWPLCWT*M**GLCGCCRPS
CQT*RGAVRDACW*PGAWED*WVSGREWPRQLQI
LCVRS*ALKAGSAGGVEWGAVSLEGHRLPGDDP
LAAAPQEPRLPT*GLPFNDVYCASKFALEGLCE
SLAVLLLPFGVQ*VNTPVPRTLLTLT*RRRAPCP
AGAALGRSPWPSLPGSH*LCARHLGWAHGTHCA
TC*PSC*SVGIVMGKLQPREVR*LAQGHTAARD
PARFES*HQGSLVASDGFGRAAPAGTRVSNVLVI
PSALSASLPSAAVLLAVGGRDVVGAGTGVGAGT
GAWLASAPSHSLLSGQQLEPDRVRPSAHRLHGE
GVGQPRGGAGPHGHPHLPPLLPIPRPQQASLSRG
GAEP*GGGGGERRAGLQEWGRCVLRRAAVATAL
LPPPQVFLTALRAPKPTLRYFTTERFLPLLRMR
LDDPSGSNYVTAMHREVFGDVPAKAEAGAEAGGG
AGPGAEDEAGRGAVGDPELGDPPAAPQ*RLPQP
LSPAPFFVPWVCVVPGDGAAVAAVGG*LR*IAL
ASFTSAARRDGCRL*CQRFGRRRQEDRSSPGVGD
QPEQHSETPISTKIKKI
Frame 2
LGGTWVGVESGCDLHSISHETSGKFLAFSECFP
FLSLFFSFLFFFCFETEFHSCCPAWSAIARSQF
TTTSASRVQV*LGLQACATTPS*FCIFSRDEVS
PCWSDWSWTPDLR*SARPGLSECWDYRHDPPHPA
L*VFLICKMGIIIPI*SVLVMVEIMPESASIEV
*YPARTVIRMNSNMTTVTFCKCVKKKEG*VNQR
ETAPLTLFCPRGLSDPHHLGSCLTRRSSWGYRTG
YFATMAHLS*AQWRVPGGQKSLIGASRVVEPLS
ELLWRSGAGEV*TDRRLGEDAAEEGGRSAPGGG
GTEAGIQLGLRGREG*GGWPFGVGRDMTTESLGS
GNRQEPAGALGALCGALGEGGRHSTLTCPYLWM
RVFSACWMMCCPCSWRKRPPSHPLLPVALPC*P
RQICYPYSIQEGHREGAQVAWVSEAWDLLSVPLS
PTPFAPL*QCVYICVHVCTCVVWLTRSPLGVG*
*EVKCDPLSSVHSFFQACEYSHC*YNLLQSHDF
E*HLDADDPPTKETPLSPTDSRSVSPTAYSIQHV
HLIQ*T*CV*TPIPHFQSSYRLLPQSSLLYYMT
TPFFPILVLKTLE*SLTPVSLTFQPIQQQILTA
LPLLIKAYPQITLFQTAHCHLSLQLQQ*PPN*FS
*FHSCPSAVKLPQSS*NDLLGWAQWLTPVIPAL
WEVEAG*SP*VGSSRPSSPTGRNPISTKNTKIS
RMWWDVSVIPTTWEAETGELLEPGRWRLQ*ARIV
PQHSSLGKKRETPSQNKIIF*K*QGQVGWHFFI
IRALWEAEVGRSLDIRGLRPAWPTW*NSVSTKN
TKN*LGVVAGACNPSYSGG*CRRIA*TRQMQDAV
SQD*DTALQPKQYFVSKIKKSLGNKVRPRLYKK
VKKLAGYGGSDGTHL*SRLLGKLFNIFCRDRVS
LCYLAWS*TPGLQQSPFLGLPKCWDYRHEPPCPA
SKHILKDRNKQPYEEIQTGRSGRVQSRSFYLHR
VGVTSPSGHILSASTRSALRSSRTLSFGPFMEN
SIGCP*LKHGQL**CDWAKRV*SKPSKASPDSLG
LCAAFLSPGYGARTHSGMRILQPTIRLESCLGQ
LKRGQEKVRETKGCFLRPEAPQHDNVRL*PWSC
EL*ARNPGRNQHIYNHLPPPNAFTFTASAANCGH
YNRSCGTEAYPGESAQGATLCPRGNPHLLVKPP
LPLTSNQDSLLFQAVGSCLFWLRTGWGERARDP
QEGLSLRLRGRHNKGWGPAGGAGRSR*YQAQSPS
LSPQSHHGPHRGAHHRLFLGHRPALGRTSGFRS
IPELQSVCHVEGPENTGPAVGGGPGPGMPSGIP
GDVAAGRKGLKIRGRCPGTRD*GPRGRAGV*RRP
GPAGAAGGAGGGRRGLCAGRECSRDCADAAGLP
ARHEEARFGTRVGDRERGRIDG*VVGSGLGSSR
FFVCGAEP*RQVPRGGWSGVPSAWRGTVCPGMTP
WPLRLRNLVSPPKGCLSMTFIAPASSRSKAYAR
VWRFCCCPLGSSESTPPFPEPS*L*PRDAEHPV
LREPLWGDLPGPLCPAHISCVPGTWAGRMARIVP
PADLAAEVLVLLWGSSSPERLGDWPKVTQRPGT
PLDSNPDTRAPL*PQMDLGGLLRQEPAFQMFWL
SPAPFPPHFPAQRCCSRSGAGTWLGLGLGLGLGL
GPGWRPPPPTRCSPASSLSLIECGPVHTAFMEK
VLGSPEEVLDRTDIHTFHRFYQYLAHSKQVFRE
AAQNPEEVAEVSAGLDSRSGGGASSGAQRWPQLS
SRRRRSSSPLCAPRSRPCATSPPSASCPCCGCA
WTTPAAPTTSPPCTGKCSATFRQRPRLGPRLGA
GPGLGQRTRPGAVRWGTLSSAILRPPRSKGFLSR
CLPRPSLSPGSVWSLGMGRR*QLWVAN*DRSR*
PVLPAQLGAMAVACNASALGGGGRRIAQAPELE
TSQSNTVRPPSLQK*RKF
Sequence HSD17B1.cAug05
AGGACCCACTCTGGAATGAGGATCCTACAACCCACAATCAGATTAGAGTCCTGCCTTGGGCAGCTGAAAAGAGGACAGGAGAAGGTCAGAGAGACGAAAG
GCTGTTTTTTGAGGCCTGAGGCACCCCAACATGACAACGTAAGACTGTAACCATGGTCATGTGAGTTATGAGCTAGGAACCCTGGACGAAACCAGCACAT
ATACAATCATCTCCCACCTCCCAACGCCTTTACTTTCACAGCCTCTGCAGCAAACTGCGGTCACTATAATCGCTCCTGTGGCACAGAGGCATACCCAGGG
GAATCTGCCCAGGGGGCCACTCTGTGCCCACGTGGGAACCCACACCTGCTTGTAAAGCCTCCCCTCCCTCTGACCAGCAACCAGGACAGTTTGTTGTTCC
AAGCAGTGGGCTCATGTCTGTTTTGGCTCAGAACAGGGTGGGGAGAGCGGGCCAGGGACCCGCAGGAAGGCTTATCCTTGAGATTGCGTGGGAGACACAA
CAAGGGGTGGGGGCCCGCAGGCGGGGCGGGGCGAAGCAGGTGATATCAAGCCCAGAGCCCCAGCCTCTCCCCACAGTCTCACCATGGCCCGCACCGTGGT
GCTCATCACCGGCTGTTCCTCGGGCATCGGCCTGCACTTGGCCGTACGTCTGGCTTCAGATCCATCCCAGAGCTTCAAAGGTATAGATAGGCAGGGACAG
GGAGGGAGAGAAGGGAGGAGCCCTTGGAGGCCAGAAGGGAAGTCAGATCTTCCTCCTCTCCCAAAACCTCCAGTGTATGCCACGTTGAGGGACCTGAAAA
CACAGGGCCGGCTGTGGGAGGCGGCCCGGGCCCTGGCATGCCCTCCGGGATCCCTGGAGACGTTGCAGCTGGACGTAAGGGACTCAAAATCCGTGGCCGC
TGCCCGGGAACGCGTGACTGAGGGCCGCGTGGACGTGCTGGGTGAGCCTCCTGGAAGCATATGGGCTCCTAGGAGCCTTCTCCGCCCTGCGTTGAAACCA
ACATGTTCCCAGGCCCAGGGAGCACGAGGGGACAGGCCGTGCTGAGGGTGATGCTGAGGCGGGCTGGTCGGGCCTCTTGTCTCCGCAGTGTGTAACGCAG
GCCTGGGCCTGCTGGGGCCGCTGGAGGCGCTGGGGGAGGACGCCGTGGCCTCTGTGCTGGACGTGAATGTAGTAGGGACTGTGCGGATGCTGCAGGCCTT
CCTGCCAGACATGAAGAGGCGCGGTTCGGGACGCGTGTTGGTGACCGGGAGCGTGGGAGGATTGATGGGTGAGTGGTAGGGAGTGGCCTCGGCAGCTCCA
GATTCTTTGTGTGCGGAGCTGAGCCTTGAAGGCAGGTTCCGCGGGGGGGGTGGAGTGGGGTGCCGTCAGCTTGGAGGGGCACCGTCTGCCCGGGGATGAC
CCCCTGGCCGCTGCGCCTCAGGAACCTCGTCTCCCCACCTAAGGGCTGCCTTTCAATGACGTTTATTGCGCCAGCAAGTTCGCGCTCGAAGGCTTATGCG
AGAGTCTGGCGGTTCTGCTGCTGCCCTTTGGGGTCCAGTGAGTCAACACCCCCGTTCCCCGAACCCTCTTAACTCTGACCTAGAGACGCCGAGCACCCTG
TCCTGCGGGAGCCGCTCTGGGGCGATCTCCCTGGCCCTCTCTGCCCGGCTCACATTAGCTGTGTGCCAGGCACTTGGGCTGGGCGCATGGCACGCATTGT
GCCACCTGCTGACCTAGCTGCTGAAGTGTTGGTATTGTTATGGGGAAGCTCCAGCCCAGAGAGGTTAGGTGACTGGCCCAAGGTCACACAGCGGCCAGGG
ACCCCGCTAGATTCGAATCCTGACACCAGGGCTCCCTTGTAGCCTCAGATGGATTTGGGAGGGCTGCTCCGGCAGGAACCCGCGTTTCAAATGTTCTGGT
TATCCCCAGCGCCCTTTCCGCCTCACTTCCCAGCGCAGCGGTGCTGCTCGCGGTCGGGGGCCGGGACGTGGTTGGGGCTGGGACTGGGGTTGGGGCTGGG
ACTGGGGCCTGGCTGGCGTCCGCCCCCTCCCACTCGTTGCTCTCCGGCCAGCAGCTTGAGCCTGATCGAGTGCGGCCCAGTGCACACCGCCTTCATGGAG
AAGGTGTTGGGCAGCCCAGAGGAGGTGCTGGACCGCACGGACATCCACACCTTCCACCGCTTCTACCAATACCTCGCCCACAGCAAGCAAGTCTTTCGCG
AGGCGGCGCAGAACCCTGAGGAGGTGGCGGAGGTCTTCCTCACCGCTTTGCGCGCCCCGAAGCCGACCCTGCGCTACTTCACCACCGAGCGCTTCCTGCC
CCTGCTGCGGATGCGCCTGGACGACCCCAGCGGCTCCAACTACGTCACCGCCATGCACCGGGAAGTGTTCGGCGACGTTCCGGCAAAGGCCGAGGCTGGG
GCCGAGGCTGGGGGCGGGGCCGGGCCTGGGGCAGAGGACGAGGCCGGGCGCGGTGCGGTGGGGGACCCTGAGCTCGGCGATCCTCCGGCCGCCCCGCAGT
AAAGGCTTCCTCAGCCGCTGTCTCCCGCGCCCTTCTTTGTCCCCTGGGTCTGTGTGGTCCCTGGGGATGGGGCGGCGGTAGCAGCTGTGGGTGGCTAATT
AAGATAGATCGCGTTAGCCAGTTTTACCAGCGCAGCTAGGCGCGATGGCTGTCGCCTGTAATGCCAGCGCTTTGGGAGGCGGAGGCAGGAGGATCGCTCA
AGCCCCGGAGTTGGAGACCAGCCAGAGCAACACAGTGAGACCCCCATCTCTACAAAAATAAAGAAAATTTAAAAATCAG
- HSD17B1.F4 (forward primer)
- HSD17B1.R4 (reverse primer)
- Intron retention
Frame 0
RTHSGMRILQPTIRLESCLGQLKRGQEKVRETK
GCFLRPEAPQHDNVRL*PWSCEL*ARNPGRNQHI
YNHLPPPNAFTFTASAANCGHYNRSCGTEAYPG
ESAQGATLCPRGNPHLLVKPPLPLTSNQDSLLF
QAVGSCLFWLRTGWGERARDPQEGLSLRLRGRHN
KGWGPAGGAGRSR*YQAQSPSLSPQSHHGPHRG
AHHRLFLGHRPALGRTSGFRSIPELQRYR*AGT
GRERREEPLEARREVRSSSSPKTSSVCHVEGPEN
TGPAVGGGPGPGMPSGIPGDVAAGRKGLKIRGR
CPGTRD*GPRGRAG*ASWKHMGS*EPSPPCVET
NMFPGPGSTRGQAVLRVMLRRAGRASCLRSV*RR
PGPAGAAGGAGGGRRGLCAGRECSRDCADAAGL
PARHEEARFGTRVGDRERGRIDG*VVGSGLGSS
RFFVCGAEP*RQVPRGGWSGVPSAWRGTVCPGMT
PWPLRLRNLVSPPKGCLSMTFIAPASSRSKAYA
RVWRFCCCPLGSSESTPPFPEPS*L*PRDAEHP
VLREPLWGDLPGPLCPAHISCVPGTWAGRMARIV
PPADLAAEVLVLLWGSSSPERLGDWPKVTQRPG
TPLDSNPDTRAPL*PQMDLGGLLRQEPAFQMFW
LSPAPFPPHFPAQRCCSRSGAGTWLGLGLGLGLG
LGPGWRPPPPTRCSPASSLSLIECGPVHTAFME
KVLGSPEEVLDRTDIHTFHRFYQYLAHSKQVFR
EAAQNPEEVAEVFLTALRAPKPTLRYFTTERFLP
LLRMRLDDPSGSNYVTAMHREVFGDVPAKAEAG
AEAGGGAGPGAEDEAGRGAVGDPELGDPPAAPQ
*RLPQPLSPAPFFVPWVCVVPGDGAAVAAVGG*L
R*IALASFTSAARRDGCRL*CQRFGRRRQEDRS
SPGVGDQPEQHSETPISTKIKKI*KS
Frame 1
GPTLE*GSYNPQSD*SPALGS*KEDRRRSERRK
AVF*GLRHPNMTT*DCNHGHVSYELGTLDETST
YTIISHLPTPLLSQPLQQTAVTIIAPVAQRHTQG
NLPRGPLCAHVGTHTCL*SLPSL*PATRTVCCS
KQWAHVCFGSEQGGESGPGTRRKAYP*DCVGDT
TRGGGPQAGRGEAGDIKPRAPASPHSLTMARTVV
LITGCSSGIGLHLAVRLASDPSQSFKGIDRQGQ
GGREGRSPWRPEGKSDLPPLPKPPVYATLRDLK
TQGRLWEAARALACPPGSLETLQLDVRDSKSVAA
ARERVTEGRVDVLGEPPGSIWAPRSLLRPALKP
TCSQAQGARGDRPC*G*C*GGLVGPLVSAVCNA
GLGLLGPLEALGEDAVASVLDVNVVGTVRMLQAF
LPDMKRRGSGRVLVTGSVGGLMGEW*GVASAAP
DSLCAELSLEGRFRGGGGVGCRQLGGAPSARG*
PPGRCASGTSSPHLRAAFQ*RLLRQQVRARRLMR
ESGGSAAALWGPVSQHPRSPNPLNSDLETPSTL
SCGSRSGAISLALSARLTLAVCQALGLGAWHAL
CHLLT*LLKCWYCYGEAPAQRG*VTGPRSHSGQG
PR*IRILTPGLPCSLRWIWEGCSGRNPRFKCSG
YPQRPFRLTSQRSGAARGRGPGRGWGWDWGWGW
DWGLAGVRPLPLVALRPAA*A*SSAAQCTPPSWR
RCWAAQRRCWTARTSTPSTASTNTSPTASKSFA
RRRRTLRRWRRSSSPLCAPRSRPCATSPPSASC
PCCGCAWTTPAAPTTSPPCTGKCSATFRQRPRLG
PRLGAGPGLGQRTRPGAVRWGTLSSAILRPPRS
KGFLSRCLPRPSLSPGSVWSLGMGRR*QLWVAN
*DRSR*PVLPAQLGAMAVACNASALGGGGRRIAQ
APELETSQSNTVRPPSLQK*RKFKNQ
Frame 2
DPLWNEDPTTHNQIRVLPWAAEKRTGEGQRDER
LFFEA*GTPT*QRKTVTMVM*VMS*EPWTKPAH
IQSSPTSQRLYFHSLCSKLRSL*SLLWHRGIPR
GICPGGHSVPTWEPTPACKASPPSDQQPGQFVVP
SSGLMSVLAQNRVGRAGQGPAGRLILEIAWETQ
QGVGARRRGGAKQVISSPEPQPLPTVSPWPAPW
CSSPAVPRASACTWPYVWLQIHPRASKV*IGRDR
EGEKGGALGGQKGSQIFLLSQNLQCMPR*GT*K
HRAGCGRRPGPWHALRDPWRRCSWT*GTQNPWP
LPGNA*LRAAWTCWVSLLEAYGLLGAFSALR*NQ
HVPRPREHEGTGRAEGDAEAGWSGLLSPQCVTQ
AWACWGRWRRWGRTPWPLCWT*M**GLCGCCRP
SCQT*RGAVRDACW*PGAWED*WVSGREWPRQLQ
ILCVRS*ALKAGSAGGVEWGAVSLEGHRLPGDD
PLAAAPQEPRLPT*GLPFNDVYCASKFALEGLC
ESLAVLLLPFGVQ*VNTPVPRTLLTLT*RRRAPC
PAGAALGRSPWPSLPGSH*LCARHLGWAHGTHC
ATC*PSC*SVGIVMGKLQPREVR*LAQGHTAAR
DPARFES*HQGSLVASDGFGRAAPAGTRVSNVLV
IPSALSASLPSAAVLLAVGGRDVVGAGTGVGAG
TGAWLASAPSHSLLSGQQLEPDRVRPSAHRLHG
EGVGQPRGGAGPHGHPHLPPLLPIPRPQQASLSR
GGAEP*GGGGGLPHRFARPEADPALLHHRALPA
PAADAPGRPQRLQLRHRHAPGSVRRRSGKGRGW
GRGWGRGRAWGRGRGRARCGGGP*ARRSSGRPAV
KASSAAVSRALLCPLGLCGPWGWGGGSSCGWLI
KIDRVSQFYQRS*ARWLSPVMPALWEAEAGGSL
KPRSWRPARATQ*DPHLYKNKENLKI