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Sequences

Sequence HSD17B1.dAug05

AGGACCCACTCTGGAATGAGGATCCTACAACCCACAATCAGATTAGAGTCCTGCCTTGGGCAGCTGAAAAGAGGACAGGAGAAGGTCAGAGAGACGAAAG GCTGTTTTTTGAGGCCTGAGGCACCCCAACATGACAACGTAAGACTGTAACCATGGTCATGTGAGTTATGAGCTAGGAACCCTGGACGAAACCAGCACAT ATACAATCATCTCCCACCTCCCAACGCCTTTACTTTCACAGCCTCTGCAGCAAACTGCGGTCACTATAATCGCTCCTGTGGCACAGAGGCATACCCAGGG GAATCTGCCCAGGGGGCCACTCTGTGCCCACGTGGGAACCCACACCTGCTTGTAAAGCCTCCCCTCCCTCTGACCAGCAACCAGGACAGTTTGTTGTTCC AAGCAGTGGGCTCATGTCTGTTTTGGCTCAGAACAGGGTGGGGAGAGCGGGCCAGGGACCCGCAGGAAGGCTTATCCTTGAGATTGCGTGGGAGACACAA CAAGGGGTGGGGGCCCGCAGGCGGGGCGGGGCGAAGCAGGTGATATCAAGCCCAGAGCCCCAGCCTCTCCCCACAGTCTCACCATGGCCCGCACCGTGGT GCTCATCACCGGCTGTTCCTCGGGCATCGGCCTGCACTTGGCCGTACGTCTGGCTTCAGATCCATCCCAGAGCTTCAAAGGTATAGATAGGCAGGGACAG GGAGGGAGAGAAGGGAGGAGCCCTTGGAGGCCAGAAGGGAAGTCAGATCTTCCTCCTCTCCCAAAACCTCCAGTGTATGCCACGTTGAGGGACCTGAAAA CACAGGGCCGGCTGTGGGAGGCGGCCCGGGCCCTGGCATGCCCTCCGGGATCCCTGGAGACGTTGCAGCTGGACGTAAGGGACTCAAAATCCGTGGCCGC TGCCCGGGAACGCGTGACTGAGGGCCGCGTGGACGTGCTGGTGTGTAACGCAGGCCTGGGCCTGCTGGGGCCGCTGGAGGCGCTGGGGGAGGACGCCGTG GCCTCTGTGCTGGACGTGAATGTAGTAGGGACTGTGCGGATGCTGCAGGCCTTCCTGCCAGACATGAAGAGGCGCGGTTCGGGACGCGTGTTGGTGACCG GGAGCGTGGGAGGATTGATGGGTGAGTGGTAGGGAGTGGCCTCGGCAGCTCCAGATTCTTTGTGTGCGGAGCTGAGCCTTGAAGGCAGGTTCCGCGGGGG GGGTGGAGTGGGGTGCCGTCAGCTTGGAGGGGCACCGTCTGCCCGGGGATGACCCCCTGGCCGCTGCGCCTCAGGAACCTCGTCTCCCCACCTAAGGGCT GCCTTTCAATGACGTTTATTGCGCCAGCAAGTTCGCGCTCGAAGGCTTATGCGAGAGTCTGGCGGTTCTGCTGCTGCCCTTTGGGGTCCACTTGAGCCTG ATCGAGTGCGGCCCAGTGCACACCGCCTTCATGGAGAAGGTGTTGGGCAGCCCAGAGGAGGTGCTGGACCGCACGGACATCCACACCTTCCACCGCTTCT ACCAATACCTCGCCCACAGCAAGCAAGTCTTTCGCGAGGCGGCGCAGAACCCTGAGGAGGTGGCGGAGGTCTTCCTCACCGCTTTGCGCGCCCCGAAGCC GACCCTGCGCTACTTCACCACCGAGCGCTTCCTGCCCCTGCTGCGGATGCGCCTGGACGACCCCAGCGGCTCCAACTACGTCACCGCCATGCACCGGGAA GTGTTCGGCGACGTTCCGGCAAAGGCCGAGGCTGGGGCCGAGGCTGGGGGCGGGGCCGGGCCTGGGGCAGAGGACGAGGCCGGGCGCGGTGCGGTGGGGG ACCCTGAGCTCGGCGATCCTCCGGCCGCCCCGCAGTAAAGGCTTCCTCAGCCGCTGTCTCCCGCGCCCTTCTTTGTCCCCTGGGTCTGTGTGGTCCCTGG GGATGGGGCGGCGGTAGCAGCTGTGGGTGGCTAATTAAGATAGATCGCGTTAGCCAGTTTTACCAGCGCAGCTAGGCGCGATGGCTGTCGCCTGTAATGC CAGCGCTTTGGGAGGCGGAGGCAGGAGGATCGCTCAAGCCCCGGAGTTGGAGACCAGCCAGAGCAACACAGTGAGACCCCCATCTCTACAAAAATAAAGA AAATTTAAAAATCAG

HSD17B1.F4 (forward primer)
HSD17B1.R4 (reverse primer)
Intron retention

Frame 0

RTHSGMRILQPTIRLESCLGQLKRGQEKVRETK GCFLRPEAPQHDNVRL*PWSCEL*ARNPGRNQHI YNHLPPPNAFTFTASAANCGHYNRSCGTEAYPG ESAQGATLCPRGNPHLLVKPPLPLTSNQDSLLF QAVGSCLFWLRTGWGERARDPQEGLSLRLRGRHN KGWGPAGGAGRSR*YQAQSPSLSPQSHHGPHRG AHHRLFLGHRPALGRTSGFRSIPELQRYR*AGT GRERREEPLEARREVRSSSSPKTSSVCHVEGPEN TGPAVGGGPGPGMPSGIPGDVAAGRKGLKIRGR CPGTRD*GPRGRAGV*RRPGPAGAAGGAGGGRR GLCAGRECSRDCADAAGLPARHEEARFGTRVGDR ERGRIDG*VVGSGLGSSRFFVCGAEP*RQVPRG GWSGVPSAWRGTVCPGMTPWPLRLRNLVSPPKG CLSMTFIAPASSRSKAYARVWRFCCCPLGST*A* SSAAQCTPPSWRRCWAAQRRCWTARTSTPSTAS TNTSPTASKSFARRRRTLRRWRRSSSPLCAPRS RPCATSPPSASCPCCGCAWTTPAAPTTSPPCTGK CSATFRQRPRLGPRLGAGPGLGQRTRPGAVRWG TLSSAILRPPRSKGFLSRCLPRPSLSPGSVWSL GMGRR*QLWVAN*DRSR*PVLPAQLGAMAVACNA SALGGGGRRIAQAPELETSQSNTVRPPSLQK*R KFKNQ

Frame 1

GPTLE*GSYNPQSD*SPALGS*KEDRRRSERRK AVF*GLRHPNMTT*DCNHGHVSYELGTLDETST YTIISHLPTPLLSQPLQQTAVTIIAPVAQRHTQG NLPRGPLCAHVGTHTCL*SLPSL*PATRTVCCS KQWAHVCFGSEQGGESGPGTRRKAYP*DCVGDT TRGGGPQAGRGEAGDIKPRAPASPHSLTMARTVV LITGCSSGIGLHLAVRLASDPSQSFKGIDRQGQ GGREGRSPWRPEGKSDLPPLPKPPVYATLRDLK TQGRLWEAARALACPPGSLETLQLDVRDSKSVAA ARERVTEGRVDVLVCNAGLGLLGPLEALGEDAV ASVLDVNVVGTVRMLQAFLPDMKRRGSGRVLVT GSVGGLMGEW*GVASAAPDSLCAELSLEGRFRGG GGVGCRQLGGAPSARG*PPGRCASGTSSPHLRA AFQ*RLLRQQVRARRLMRESGGSAAALWGPLEP DRVRPSAHRLHGEGVGQPRGGAGPHGHPHLPPLL PIPRPQQASLSRGGAEP*GGGGGLPHRFARPEA DPALLHHRALPAPAADAPGRPQRLQLRHRHAPG SVRRRSGKGRGWGRGWGRGRAWGRGRGRARCGGG P*ARRSSGRPAVKASSAAVSRALLCPLGLCGPW GWGGGSSCGWLIKIDRVSQFYQRS*ARWLSPVM PALWEAEAGGSLKPRSWRPARATQ*DPHLYKNKE NLKI

Frame 2

DPLWNEDPTTHNQIRVLPWAAEKRTGEGQRDER LFFEA*GTPT*QRKTVTMVM*VMS*EPWTKPAH IQSSPTSQRLYFHSLCSKLRSL*SLLWHRGIPR GICPGGHSVPTWEPTPACKASPPSDQQPGQFVVP SSGLMSVLAQNRVGRAGQGPAGRLILEIAWETQ QGVGARRRGGAKQVISSPEPQPLPTVSPWPAPW CSSPAVPRASACTWPYVWLQIHPRASKV*IGRDR EGEKGGALGGQKGSQIFLLSQNLQCMPR*GT*K HRAGCGRRPGPWHALRDPWRRCSWT*GTQNPWP LPGNA*LRAAWTCWCVTQAWACWGRWRRWGRTPW PLCWT*M**GLCGCCRPSCQT*RGAVRDACW*P GAWED*WVSGREWPRQLQILCVRS*ALKAGSAG GVEWGAVSLEGHRLPGDDPLAAAPQEPRLPT*GL PFNDVYCASKFALEGLCESLAVLLLPFGVHLSL IECGPVHTAFMEKVLGSPEEVLDRTDIHTFHRF YQYLAHSKQVFREAAQNPEEVAEVFLTALRAPKP TLRYFTTERFLPLLRMRLDDPSGSNYVTAMHRE VFGDVPAKAEAGAEAGGGAGPGAEDEAGRGAVG DPELGDPPAAPQ*RLPQPLSPAPFFVPWVCVVPG DGAAVAAVGG*LR*IALASFTSAARRDGCRL*C QRFGRRRQEDRSSPGVGDQPEQHSETPISTKIK KI*KS

Sequence HSD17B1.aAug05

AGTTAGGTGGGACTTGGGTTGGGGTAGAGTCAGGTTGTGACCTGCACTCCATTAGCCATGAGACCTCAGGCAAGTTCCTTGCTTTCTCTGAGTGCTTTCC TTTCCTTTCCCTTTTCTTTTCTTTTTTGTTTTTCTTTTGTTTTGAGACGGAGTTTCACTCTTGTTGCCCAGCCTGGAGTGCAATAGCGCGGTCTCAGTTC ACCACAACCTCTGCCTCCCGGGTTCAAGTGTAGCTAGGATTGCAGGCATGTGCCACCACGCCCAGCTAATTTTGTATTTTTAGTAGAGACGAGGTTTCTC CATGTTGGTCAGACTGGTCTTGGACTCCCGACCTCAGGTGATCCGCCCGCCCTGGCCTCTCAGAGTGCTGGGATTACAGGCATGACCCACCCCATCCGGC CCTCTGAGTCTTTCTTATCTGTAAAATGGGTATAATAATACCTATCTAATCGGTTTTAGTAATGGTGGAGATAATGCCTGAAAGTGCTAGTATAGAGGTT TAATACCCAGCAAGGACTGTTATTAGAATGAATAGTAATATGACTACTGTCACATTTTGCAAATGTGTAAAGAAGAAAGAGGGCTAAGTGAATCAAAGGG AGACAGCCCCACTCACCCTGTTCTGCCCCAGAGGACTGAGCGATCCCCACCATCTGGGAAGCTGCCTCACCAGGAGGTCCAGCTGGGGCTACAGGACTGG CTACTTTGCTACAATGGCCCATCTTTCCTGAGCCCAGTGGAGGGTCCCAGGGGGGCAGAAGTCATTGATAGGGGCCAGTAGGGTTGTAGAGCCACTGTCT GAACTTCTGTGGAGGTCTGGTGCAGGGGAGGTGTGAACAGATAGGAGGCTAGGTGAGGATGCAGCAGAGGAAGGGGGCAGGAGTGCCCCAGGAGGGGGCG GTACCGAGGCAGGGATTCAGCTGGGTCTGAGAGGGAGGGAAGGCTGAGGGGGATGGCCCTTTGGAGTGGGCAGGGACATGACCACAGAAAGCCTGGGAAG TGGAAACAGACAGGAGCCTGCTGGAGCTCTTGGAGCTTTGTGTGGGGCACTAGGGGAAGGAGGCAGGCACTCCACCCTGACCTGCCCCTACCTCTGGATG AGGGTCTTCTCTGCCTGTTGGATGATGTGCTGCCCCTGCTCTTGGAGGAAGAGGCCTCCCAGCCACCCCCTCCTGCCAGTCGCCTTGCCCTGCTGACCCA GACAGATCTGCTATCCCTACAGCATCCAGGAAGGCCACAGGGAGGGGGCCCAGGTGGCATGGGTTTCTGAGGCCTGGGATCTGCTCTCAGTCCCGCTCTC GCCAACCCCCTTTGCCCCTCTGTGACAGTGTGTGTACATATGCGTGCATGTGTGTACCTGTGTGGTGTGGCTCACAAGGTCACCCCTGGGGGTGGGATAA TAAGAGGTAAAGTGTGACCCCCTCTCTTCCGTACATTCATTTTTTCAAGCTTGTGAATATTCACATTGTTAATATAATCTTCTGCAGTCCCATGACTTCG AATAGCACTTAGATGCCGATGACCCCCCCACCAAAGAAACCCCACTGTCTCCCACAGACTCCAGGTCTGTATCTCCAACTGCCTACTCAATACAACACGT CCACCTGATACAATGAACTTAATGTGTCTAAACCCCAATTCCACATTTCCAGTCCTCCTACCGGCTCCTCCCCCAGTCTTCTCTACTTTACTATATGACA ACTCCATTTTTCCCAATACTGGTTTTAAAAACCCTGGAGTAGTCCTTGACTCCAGTCTCTCTCACCTTTCAACCAATTCAGCAGCAAATACTGACAGCTC TACCTTTGCTCATCAAAGCATATCCCCAAATCACCCTATTCCAGACTGCACACTGTCATCTCTCCCTTCAATTACAGCAGTAGCCTCCTAACTAGTTTTC TTGATTCCACTCCTGCCCATCAGCAGTGAAATTACCCCAGAGCAGTTAAAATGATCTTTTAGGATGGGCACAGTGGCTCACGCCTGTAATACCAGCACTT TGGGAGGTGGAGGCTGGCTGATCCCCTTAGGTTGGGAGTTCAAGACCATCCTCACCAACAGGGAGAAACCCCATCTCTACTAAAAATACAAAAATTAGCC GGATGTGGTGGGACGTGTCTGTAATCCCAACTACTTGGGAGGCTGAGACAGGAGAACTGCTTGAACCCGGGAGGTGGAGGTTGCAGTGAGCCAGAATCGT GCCACAGCACTCCAGCCTGGGCAAGAAGCGCGAAACTCCGTCTCAAAATAAAATAATCTTCTAAAAATGACAGGGCCAGGTGGGGTGGCACTTTTTTATA ATCCGAGCACTTTGGGAGGCTGAGGTGGGCAGATCGCTTGACATCAGGGGTTTGAGACCAGCCTGGCCAACATGGTGAAACTCCGTCTCTACTAAAAATA CTAAAAATTAGCTGGGCGTGGTGGCGGGTGCCTGTAATCCCAGCTACTCGGGAGGCTGATGCAGGAGAATCGCCTGAACCAGGCAGATGCAGGATGCAGT GAGCCAAGATTAAGACACTGCACTCCAACCTAAGCAATACTTTGTCTCAAAAATAAAAAAAAGCCTGGGAAACAAAGTGAGACCCCGTCTCTACAAAAAA GTCAAAAAATTAGCTGGGTATGGTGGCAGTGATGGCACACACCTGTAGTCCCGGCTACTTGGGAAGCTTTTTAATATTTTTTGCAGAGACCGGGTCTCAC TCTGTTACCTGGCCTGGTCTTGAACTCCTGGGCTCCAACAATCCCCTTTCCTGGGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACCGTGCCCAGC CTCAAAGCATATTTTAAAGGATAGAAATAAACAGCCATATGAAGAGATACAGACAGGGCGGTCTGGAAGGGTCCAGAGCAGGAGCTTCTATCTCCATAGA GTTGGGGTTACGTCACCCTCTGGGCACATTCTGTCAGCCTCCACACGTTCAGCCCTCAGAAGCTCCCGAACCCTGTCCTTTGGGCCTTTTATGGAGAACT CCATTGGCTGTCCATGACTGAAGCATGGACAACTGTGATAATGTGATTGGGCAAAAAGGGTCTGATCTAAGCCCAGCAAGGCCAGTCCAGATTCTTTGGG CCTTTGTGCAGCATTCCTTTCTCCAGGGTATGGGGCAAGGACCCACTCTGGAATGAGGATCCTACAACCCACAATCAGATTAGAGTCCTGCCTTGGGCAG CTGAAAAGAGGACAGGAGAAGGTCAGAGAGACGAAAGGCTGTTTTTTGAGGCCTGAGGCACCCCAACATGACAACGTAAGACTGTAACCATGGTCATGTG AGTTATGAGCTAGGAACCCTGGACGAAACCAGCACATATACAATCATCTCCCACCTCCCAACGCCTTTACTTTCACAGCCTCTGCAGCAAACTGCGGTCA CTATAATCGCTCCTGTGGCACAGAGGCATACCCAGGGGAATCTGCCCAGGGGGCCACTCTGTGCCCACGTGGGAACCCACACCTGCTTGTAAAGCCTCCC CTCCCTCTGACCAGCAACCAGGACAGTTTGTTGTTCCAAGCAGTGGGCTCATGTCTGTTTTGGCTCAGAACAGGGTGGGGAGAGCGGGCCAGGGACCCGC AGGAAGGCTTATCCTTGAGATTGCGTGGGAGACACAACAAGGGGTGGGGGCCCGCAGGCGGGGCGGGGCGAAGCAGGTGATATCAAGCCCAGAGCCCCAG CCTCTCCCCACAGTCTCACCATGGCCCGCACCGTGGTGCTCATCACCGGCTGTTCCTCGGGCATCGGCCTGCACTTGGCCGTACGTCTGGCTTCAGATCC ATCCCAGAGCTTCAAAGTGTATGCCACGTTGAGGGACCTGAAAACACAGGGCCGGCTGTGGGAGGCGGCCCGGGCCCTGGCATGCCCTCCGGGATCCCTG GAGACGTTGCAGCTGGACGTAAGGGACTCAAAATCCGTGGCCGCTGCCCGGGAACGCGTGACTGAGGGCCGCGTGGACGTGCTGGTGTGTAACGCAGGCC TGGGCCTGCTGGGGCCGCTGGAGGCGCTGGGGGAGGACGCCGTGGCCTCTGTGCTGGACGTGAATGTAGTAGGGACTGTGCGGATGCTGCAGGCCTTCCT GCCAGACATGAAGAGGCGCGGTTCGGGACGCGTGTTGGTGACCGGGAGCGTGGGAGGATTGATGGGGCTGCCTTTCAATGACGTTTATTGCGCCAGCAAG TTCGCGCTCGAAGGCTTATGCGAGAGTCTGGCGGTTCTGCTGCTGCCCTTTGGGGTCCACTTGAGCCTGATCGAGTGCGGCCCAGTGCACACCGCCTTCA TGGAGAAGGTGTTGGGCAGCCCAGAGGAGGTGCTGGACCGCACGGACATCCACACCTTCCACCGCTTCTACCAATACCTCGCCCACAGCAAGCAAGTCTT TCGCGAGGCGGCGCAGAACCCTGAGGAGGTGGCGGAGGTCTTCCTCACCGCTTTGCGCGCCCCGAAGCCGACCCTGCGCTACTTCACCACCGAGCGCTTC CTGCCCCTGCTGCGGATGCGCCTGGACGACCCCAGCGGCTCCAACTACGTCACCGCCATGCACCGGGAAGTGTTCGGCGACGTTCCGGCAAAGGCCGAGG CTGGGGCCGAGGCTGGGGGCGGGGCCGGGCCTGGGGCAGAGGACGAGGCCGGGCGCGGTGCGGTGGGGGACCCTGAGCTCGGCGATCCTCCGGCCGCCCC GCAGTAAAGGCTTCCTCAGCCGCTGTCTCCCGCGCCCTTCTTTGTCCCCTGGGTCTGTGTGGTCCCTGGGGATGGGGCGGCGGTAGCAGCTGTGGGTGGC TAATTAAGATAGATCGCGTTAGCCAGTTTTACCAGCGCAGCTAGGCGCGATGGCTGTCGCCTGTAATGCCAGCGCTTTGGGAGGCGGAGGCAGGAGGATC GCTCAAGCCCCGGAGTTGGAGACCAGCCAGAGCAACACAGTGAGACCCCCATCTCTACAAAAATAAAGAAAATTTAAAAATCAG

HSD17B1.F4 (forward primer)
HSD17B1.R4 (reverse primer)
Intron retention

Frame 0

S*VGLGLG*SQVVTCTPLAMRPQASSLLSLSAF LSFPFSFLFCFSFVLRRSFTLVAQPGVQ*RGLSS PQPLPPGFKCS*DCRHVPPRPANFVFLVETRFL HVGQTGLGLPTSGDPPALASQSAGITGMTHPIR PSESFLSVKWV**YLSNRF**WWR*CLKVLV*RF NTQQGLLLE*IVI*LLSHFANV*RRKRAK*IKG RQPHSPCSAPED*AIPTIWEAASPGGPAGATGL ATLLQWPIFPEPSGGSQGGRSH**GPVGL*SHCL NFCGGLVQGRCEQIGG*VRMQQRKGAGVPQEGA VPRQGFSWV*EGGKAEGDGPLEWAGT*PQKAWE VETDRSLLELLELCVGH*GKEAGTPP*PAPTSG* GSSLPVG*CAAPALGGRGLPATPSCQSPCPADP DRSAIPTASRKATGRGPRWHGFLRPGICSQSRS RQPPLPLCDSVCTYACMCVPVWCGSQGHPWGWDN KR*SVTPSLPYIHFFKLVNIHIVNIIFCSPMTS NST*MPMTPPPKKPHCLPQTPGLYLQLPTQYNT ST*YNELNVSKPQFHISSPPTGSSPSLLYFTI*Q LHFSQYWF*KPWSSP*LQSLSPFNQFSSKY*QL YLCSSKHIPKSPYSRLHTVISPFNYSSSLLTSF LDSTPAHQQ*NYPRAVKMIF*DGHSGSRL*YQHF GRWRLADPLRLGVQDHPHQQGETPSLLKIQKLA GCGGTCL*SQLLGRLRQENCLNPGGGGCSEPES CHSTPAWARSAKLRLKIK*SSKNDRARWGGTFL* SEHFGRLRWADRLTSGV*DQPGQHGETPSLLKI LKISWAWWRVPVIPATREADAGESPEPGRCRMQ *AKIKTLHSNLSNTLSQK*KKAWETK*DPVSTKK SKN*LGMVAVMAHTCSPGYLGSFLIFFAETGSH SVTWPGLELLGSNNPLSWASQSAGITGMSHRAQ PQSIF*RIEINSHMKRYRQGGLEGSRAGASISIE LGLRHPLGTFCQPPHVQPSEAPEPCPLGLLWRT PLAVHD*SMDNCDNVIGQKGSDLSPARPVQILW AFVQHSFLQGMGQGPTLE*GSYNPQSD*SPALGS *KEDRRRSERRKAVF*GLRHPNMTT*DCNHGHV SYELGTLDETSTYTIISHLPTPLLSQPLQQTAV TIIAPVAQRHTQGNLPRGPLCAHVGTHTCL*SLP SL*PATRTVCCSKQWAHVCFGSEQGGESGPGTR RKAYP*DCVGDTTRGGGPQAGRGEAGDIKPRAP ASPHSLTMARTVVLITGCSSGIGLHLAVRLASDP SQSFKVYATLRDLKTQGRLWEAARALACPPGSL ETLQLDVRDSKSVAAARERVTEGRVDVLVCNAG LGLLGPLEALGEDAVASVLDVNVVGTVRMLQAFL PDMKRRGSGRVLVTGSVGGLMGLPFNDVYCASK FALEGLCESLAVLLLPFGVHLSLIECGPVHTAF MEKVLGSPEEVLDRTDIHTFHRFYQYLAHSKQVF REAAQNPEEVAEVFLTALRAPKPTLRYFTTERF LPLLRMRLDDPSGSNYVTAMHREVFGDVPAKAE AGAEAGGGAGPGAEDEAGRGAVGDPELGDPPAAP Q*RLPQPLSPAPFFVPWVCVVPGDGAAVAAVGG *LR*IALASFTSAARRDGCRL*CQRFGRRRQED RSSPGVGDQPEQHSETPISTKIKKI*KS

Frame 1

VRWDLGWGRVRL*PALH*P*DLRQVPCFL*VLS FPFPFLFFFVFLLF*DGVSLLLPSLECNSAVSV HHNLCLPGSSVARIAGMCHHAQLILYF**RRGFS MLVRLVLDSRPQVIRPPWPLRVLGLQA*PTPSG PLSLSYL*NGYNNTYLIGFSNGGDNA*KC*YRG LIPSKDCY*NE**YDYCHILQMCKEERGLSESKG DSPTHPVLPQRTERSPPSGKLPHQEVQLGLQDW LLCYNGPSFLSPVEGPRGAEVIDRGQ*GCRATV *TSVEVWCRGGVNR*EAR*GCSRGRGQECPRRGR YRGRDSAGSEREGRLRGMALWSGQGHDHRKPGK WKQTGACWSSWSFVWGTRGRRQALHPDLPLPLD EGLLCLLDDVLPLLLEEEASQPPPPASRLALLTQ TDLLSLQHPGRPQGGGPGGMGF*GLGSALSPAL ANPLCPSVTVCVHMRACVYLCGVAHKVTPGGGI IRGKV*PPLFRTFIFSSL*IFTLLI*SSAVP*LR IALRCR*PPHQRNPTVSHRLQVCISNCLLNTTR PPDTMNLMCLNPNSTFPVLLPAPPPVFSTLLYD NSIFPNTGFKNPGVVLDSSLSHLSTNSAANTDSS TFAHQSISPNHPIPDCTLSSLPSITAVAS*LVF LIPLLPISSEITPEQLK*SFRMGTVAHACNTST LGGGGWLIPLGWEFKTILTNREKPHLY*KYKN*P DVVGRVCNPNYLGG*DRRTA*TREVEVAVSQNR ATALQPGQEARNSVSK*NNLLKMTGPGGVALFY NPSTLGG*GGQIA*HQGFETSLANMVKLRLY*KY *KLAGRGGGCL*SQLLGRLMQENRLNQADAGCS EPRLRHCTPT*AILCLKNKKKPGKQSETPSLQK SQKISWVWWQ*WHTPVVPATWEAF*YFLQRPGLT LLPGLVLNSWAPTIPFPGPPKVLGLQA*ATVPS LKAYFKG*K*TAI*RDTDRAVWKGPEQELLSP* SWGYVTLWAHSVSLHTFSPQKLPNPVLWAFYGEL HWLSMTEAWTTVIM*LGKKGLI*AQQGQSRFFG PLCSIPFSRVWGKDPLWNEDPTTHNQIRVLPWA AEKRTGEGQRDERLFFEA*GTPT*QRKTVTMVM* VMS*EPWTKPAHIQSSPTSQRLYFHSLCSKLRS L*SLLWHRGIPRGICPGGHSVPTWEPTPACKAS PPSDQQPGQFVVPSSGLMSVLAQNRVGRAGQGPA GRLILEIAWETQQGVGARRRGGAKQVISSPEPQ PLPTVSPWPAPWCSSPAVPRASACTWPYVWLQI HPRASKCMPR*GT*KHRAGCGRRPGPWHALRDPW RRCSWT*GTQNPWPLPGNA*LRAAWTCWCVTQA WACWGRWRRWGRTPWPLCWT*M**GLCGCCRPS CQT*RGAVRDACW*PGAWED*WGCLSMTFIAPAS SRSKAYARVWRFCCCPLGST*A*SSAAQCTPPS WRRCWAAQRRCWTARTSTPSTASTNTSPTASKS FARRRRTLRRWRRSSSPLCAPRSRPCATSPPSAS CPCCGCAWTTPAAPTTSPPCTGKCSATFRQRPR LGPRLGAGPGLGQRTRPGAVRWGTLSSAILRPP RSKGFLSRCLPRPSLSPGSVWSLGMGRR*QLWVA N*DRSR*PVLPAQLGAMAVACNASALGGGGRRI AQAPELETSQSNTVRPPSLQK*RKFKNQ

Frame 2

LGGTWVGVESGCDLHSISHETSGKFLAFSECFP FLSLFFSFLFFFCFETEFHSCCPAWSAIARSQF TTTSASRVQV*LGLQACATTPS*FCIFSRDEVS PCWSDWSWTPDLR*SARPGLSECWDYRHDPPHPA L*VFLICKMGIIIPI*SVLVMVEIMPESASIEV *YPARTVIRMNSNMTTVTFCKCVKKKEG*VNQR ETAPLTLFCPRGLSDPHHLGSCLTRRSSWGYRTG YFATMAHLS*AQWRVPGGQKSLIGASRVVEPLS ELLWRSGAGEV*TDRRLGEDAAEEGGRSAPGGG GTEAGIQLGLRGREG*GGWPFGVGRDMTTESLGS GNRQEPAGALGALCGALGEGGRHSTLTCPYLWM RVFSACWMMCCPCSWRKRPPSHPLLPVALPC*P RQICYPYSIQEGHREGAQVAWVSEAWDLLSVPLS PTPFAPL*QCVYICVHVCTCVVWLTRSPLGVG* *EVKCDPLSSVHSFFQACEYSHC*YNLLQSHDF E*HLDADDPPTKETPLSPTDSRSVSPTAYSIQHV HLIQ*T*CV*TPIPHFQSSYRLLPQSSLLYYMT TPFFPILVLKTLE*SLTPVSLTFQPIQQQILTA LPLLIKAYPQITLFQTAHCHLSLQLQQ*PPN*FS *FHSCPSAVKLPQSS*NDLLGWAQWLTPVIPAL WEVEAG*SP*VGSSRPSSPTGRNPISTKNTKIS RMWWDVSVIPTTWEAETGELLEPGRWRLQ*ARIV PQHSSLGKKRETPSQNKIIF*K*QGQVGWHFFI IRALWEAEVGRSLDIRGLRPAWPTW*NSVSTKN TKN*LGVVAGACNPSYSGG*CRRIA*TRQMQDAV SQD*DTALQPKQYFVSKIKKSLGNKVRPRLYKK VKKLAGYGGSDGTHL*SRLLGKLFNIFCRDRVS LCYLAWS*TPGLQQSPFLGLPKCWDYRHEPPCPA SKHILKDRNKQPYEEIQTGRSGRVQSRSFYLHR VGVTSPSGHILSASTRSALRSSRTLSFGPFMEN SIGCP*LKHGQL**CDWAKRV*SKPSKASPDSLG LCAAFLSPGYGARTHSGMRILQPTIRLESCLGQ LKRGQEKVRETKGCFLRPEAPQHDNVRL*PWSC EL*ARNPGRNQHIYNHLPPPNAFTFTASAANCGH YNRSCGTEAYPGESAQGATLCPRGNPHLLVKPP LPLTSNQDSLLFQAVGSCLFWLRTGWGERARDP QEGLSLRLRGRHNKGWGPAGGAGRSR*YQAQSPS LSPQSHHGPHRGAHHRLFLGHRPALGRTSGFRS IPELQSVCHVEGPENTGPAVGGGPGPGMPSGIP GDVAAGRKGLKIRGRCPGTRD*GPRGRAGV*RRP GPAGAAGGAGGGRRGLCAGRECSRDCADAAGLP ARHEEARFGTRVGDRERGRIDGAAFQ*RLLRQQ VRARRLMRESGGSAAALWGPLEPDRVRPSAHRLH GEGVGQPRGGAGPHGHPHLPPLLPIPRPQQASL SRGGAEP*GGGGGLPHRFARPEADPALLHHRAL PAPAADAPGRPQRLQLRHRHAPGSVRRRSGKGRG WGRGWGRGRAWGRGRGRARCGGGP*ARRSSGRP AVKASSAAVSRALLCPLGLCGPWGWGGGSSCGW LIKIDRVSQFYQRS*ARWLSPVMPALWEAEAGGS LKPRSWRPARATQ*DPHLYKNKENLKI

Sequence HSD17B1.shed1.aAug05

AGGACCCACTCTGGAATGAGGATCCTACAACCCACAATCAGATTAGAGTCCTGCCTTGGGCAGCTGAAAAGAGGACAGGAGAAGGTCAGAGAGACGAAAG GCTGTTTTTTGAGGCCTGAGGCACCCCAACATGACAACGTAAGACTGTAACCATGGTCATGTGAGTTATGAGCTAGGAACCCTGGACGAAACCAGCACAT ATACAATCATCTCCCACCTCCCAACGCCTTTACTTTCACAGCCTCTGCAGCAAACTGCGGTCACTATAATCGCTCCTGTGGCACAGAGGCATACCCAGGG GAATCTGCCCAGGGGGCCACTCTGTGCCCACGTGGGAACCCACACCTGCTTGTAAAGCCTCCCCTCCCTCTGACCAGCAACCAGGACAGTTTGTTGTTCC AAGCAGTGGGCTCATGTCTGTTTTGGCTCAGAACAGGGTGGGGAGAGCGGGCCAGGGACCCGCAGGAAGGCTTATCCTTGAGATTGCGTGGGAGACACAA CAAGGGGTGGGGGCCCGCAGGCGGGGCGGGGCGAAGCAGGTGATATCAAGCCCAGAGCCCCAGCCTCTCCCCACAGTCTCACCATGGCCCGCACCGTGGT GCTCATCACCGGCTGTTCCTCGGGCATCGGCCTGCACTTGGCCGTACGTCTGGCTTCAGATCCATCCCAGAGCTTCAAAGGTATAGATAGGCAGGGACAG GGAGGGAGAGAAGGGAGGAGCCCTTGGAGGCCAGAAGGGAAGTCAGATCTTCCTCCTCTCCCAAAACCTCCAGTGTATGCCACGTTGAGGGACCTGAAAA CACAGGGCCGGCTGTGGGAGGCGGCCCGGGCCCTGGCATGCCCTCCGGGATCCCTGGAGACGTTGCAGCTGGACGTAAGGGACTCAAAATCCGTGGCCGC TGCCCGGGAACGCGTGACTGAGGGCCGCGTGGACGTGCTGGGTGAGCCTCCTGGAAGCATATGGGCTCCTAGGAGCCTTCTCCGCCCTGCGTTGAAACCA ACATGTTCCCAGGCCCAGGGAGCACGAGGGGACAGGCCGTGCTGAGGGTGATGCTGAGGCGGGCTGGTCGGGCCTCTTGTCTCCGCAGTGTGTAACGCAG GCCTGGGCCTGCTGGGGCCGCTGGAGGCGCTGGGGGAGGACGCCGTGGCCTCTGTGCTGGACGTGAATGTAGTAGGGACTGTGCGGATGCTGCAGGCCTT CCTGCCAGACATGAAGAGGCGCGGTTCGGGACGCGTGTTGGTGACCGGGAGCGTGGGAGGATTGATGGGTGAGTGGTAGGGAGTGGCCTCGGCAGCTCCA GATTCTTTGTGTGCGGAGCTGAGCCTTGAAGGCAGGTTCCGCGGGGGGGGTGGAGTGGGGTGCCGTCAGCTTGGAGGGGCACCGTCTGCCCGGGGATGAC CCCCTGGCCGCTGCGCCTCAGGAACCTCGTCTCCCCACCTAAGGGCTGCCTTTCAATGACGTTTATTGCGCCAGCAAGTTCGCGCTCGAAGGCTTATGCG AGAGTCTGGCGGTTCTGCTGCTGCCCTTTGGGGTCCAGTGAGTCAACACCCCCGTTCCCCGAACCCTCTTAACTCTGACCTAGAGACGCCGAGCACCCTG TCCTGCGGGAGCCGCTCTGGGGCGATCTCCCTGGCCCTCTCTGCCCGGCTCACATTAGCTGTGTGCCAGGCACTTGGGCTGGGCGCATGGCACGCATTGT GCCACCTGCTGACCTAGCTGCTGAAGTGTTGGTATTGTTATGGGGAAGCTCCAGCCCAGAGAGGTTAGGTGACTGGCCCAAGGTCACACAGCGGCCAGGG ACCCCGCTAGATTCGAATCCTGACACCAGGGCTCCCTTGTAGCCTCAGATGGATTTGGGAGGGCTGCTCCGGCAGGAACCCGCGTTTCAAATGTTCTGGT TATCCCCAGCGCCCTTTCCGCCTCACTTCCCAGCGCAGCGGTGCTGCTCGCGGTCGGGGGCCGGGACGTGGTTGGGGCTGGGACTGGGGTTGGGGCTGGG ACTGGGGCCTGGCTGGCGTCCGCCCCCTCCCACTCGTTGCTCTCCGGCCAGCAGCTTGAGCCTGATCGAGTGCGGCCCAGTGCACACCGCCTTCATGGAG AAGGTGTTGGGCAGCCCAGAGGAGGTGCTGGACCGCACGGACATCCACACCTTCCACCGCTTCTACCAATACCTCGCCCACAGCAAGCAAGTCTTTCGCG AGGCGGCGCAGAACCCTGAGGAGGTGGCGGAGGTGAGCGCCGGGCTGGACTCCAGGAGTGGGGGCGGTGCGTCCTCCGGCGCGCAGCGGTGGCCACAGCT CTCCTCCCGCCGCCGCAGGTCTTCCTCACCGCTTTGCGCGCCCCGAAGCCGACCCTGCGCTACTTCACCACCGAGCGCTTCCTGCCCCTGCTGCGGATGC GCCTGGACGACCCCAGCGGCTCCAACTACGTCACCGCCATGCACCGGGAAGTGTTCGGCGACGTTCCGGCAAAGGCCGAGGCTGGGGCCGAGGCTGGGGG CGGGGCCGGGCCTGGGGCAGAGGACGAGGCCGGGCGCGGTGCGGTGGGGGACCCTGAGCTCGGCGATCCTCCGGCCGCCCCGCAGTAAAGGCTTCCTCAG CCGCTGTCTCCCGCGCCCTTCTTTGTCCCCTGGGTCTGTGTGGTCCCTGGGGATGGGGCGGCGGTAGCAGCTGTGGGTGGCTAATTAAGATAGATCGCGT TAGCCAGTTTTACCAGCGCAGCTAGGCGCGATGGCTGTCGCCTGTAATGCCAGCGCTTTGGGAGGCGGAGGCAGGAGGATCGCTCAAGCCCCGGAGTTGG AGACCAGCCAGAGCAACACAGTGAGACCCCCATCTCTACAAAAATAAAGAAAATTT

HSD17B1.F4 (forward primer)
HSD17B1.R4 (reverse primer)
Intron retention

Frame 0

RTHSGMRILQPTIRLESCLGQLKRGQEKVRETK GCFLRPEAPQHDNVRL*PWSCEL*ARNPGRNQHI YNHLPPPNAFTFTASAANCGHYNRSCGTEAYPG ESAQGATLCPRGNPHLLVKPPLPLTSNQDSLLF QAVGSCLFWLRTGWGERARDPQEGLSLRLRGRHN KGWGPAGGAGRSR*YQAQSPSLSPQSHHGPHRG AHHRLFLGHRPALGRTSGFRSIPELQRYR*AGT GRERREEPLEARREVRSSSSPKTSSVCHVEGPEN TGPAVGGGPGPGMPSGIPGDVAAGRKGLKIRGR CPGTRD*GPRGRAG*ASWKHMGS*EPSPPCVET NMFPGPGSTRGQAVLRVMLRRAGRASCLRSV*RR PGPAGAAGGAGGGRRGLCAGRECSRDCADAAGL PARHEEARFGTRVGDRERGRIDG*VVGSGLGSS RFFVCGAEP*RQVPRGGWSGVPSAWRGTVCPGMT PWPLRLRNLVSPPKGCLSMTFIAPASSRSKAYA RVWRFCCCPLGSSESTPPFPEPS*L*PRDAEHP VLREPLWGDLPGPLCPAHISCVPGTWAGRMARIV PPADLAAEVLVLLWGSSSPERLGDWPKVTQRPG TPLDSNPDTRAPL*PQMDLGGLLRQEPAFQMFW LSPAPFPPHFPAQRCCSRSGAGTWLGLGLGLGLG LGPGWRPPPPTRCSPASSLSLIECGPVHTAFME KVLGSPEEVLDRTDIHTFHRFYQYLAHSKQVFR EAAQNPEEVAEVSAGLDSRSGGGASSGAQRWPQL SSRRRRSSSPLCAPRSRPCATSPPSASCPCCGC AWTTPAAPTTSPPCTGKCSATFRQRPRLGPRLG AGPGLGQRTRPGAVRWGTLSSAILRPPRSKGFLS RCLPRPSLSPGSVWSLGMGRR*QLWVAN*DRSR *PVLPAQLGAMAVACNASALGGGGRRIAQAPEL ETSQSNTVRPPSLQK*RKF

Frame 1

GPTLE*GSYNPQSD*SPALGS*KEDRRRSERRK AVF*GLRHPNMTT*DCNHGHVSYELGTLDETST YTIISHLPTPLLSQPLQQTAVTIIAPVAQRHTQG NLPRGPLCAHVGTHTCL*SLPSL*PATRTVCCS KQWAHVCFGSEQGGESGPGTRRKAYP*DCVGDT TRGGGPQAGRGEAGDIKPRAPASPHSLTMARTVV LITGCSSGIGLHLAVRLASDPSQSFKGIDRQGQ GGREGRSPWRPEGKSDLPPLPKPPVYATLRDLK TQGRLWEAARALACPPGSLETLQLDVRDSKSVAA ARERVTEGRVDVLGEPPGSIWAPRSLLRPALKP TCSQAQGARGDRPC*G*C*GGLVGPLVSAVCNA GLGLLGPLEALGEDAVASVLDVNVVGTVRMLQAF LPDMKRRGSGRVLVTGSVGGLMGEW*GVASAAP DSLCAELSLEGRFRGGGGVGCRQLGGAPSARG* PPGRCASGTSSPHLRAAFQ*RLLRQQVRARRLMR ESGGSAAALWGPVSQHPRSPNPLNSDLETPSTL SCGSRSGAISLALSARLTLAVCQALGLGAWHAL CHLLT*LLKCWYCYGEAPAQRG*VTGPRSHSGQG PR*IRILTPGLPCSLRWIWEGCSGRNPRFKCSG YPQRPFRLTSQRSGAARGRGPGRGWGWDWGWGW DWGLAGVRPLPLVALRPAA*A*SSAAQCTPPSWR RCWAAQRRCWTARTSTPSTASTNTSPTASKSFA RRRRTLRRWRR*APGWTPGVGAVRPPARSGGHS SPPAAAGLPHRFARPEADPALLHHRALPAPAADA PGRPQRLQLRHRHAPGSVRRRSGKGRGWGRGWG RGRAWGRGRGRARCGGGP*ARRSSGRPAVKASS AAVSRALLCPLGLCGPWGWGGGSSCGWLIKIDRV SQFYQRS*ARWLSPVMPALWEAEAGGSLKPRSW RPARATQ*DPHLYKNKEN

Frame 2

DPLWNEDPTTHNQIRVLPWAAEKRTGEGQRDER LFFEA*GTPT*QRKTVTMVM*VMS*EPWTKPAH IQSSPTSQRLYFHSLCSKLRSL*SLLWHRGIPR GICPGGHSVPTWEPTPACKASPPSDQQPGQFVVP SSGLMSVLAQNRVGRAGQGPAGRLILEIAWETQ QGVGARRRGGAKQVISSPEPQPLPTVSPWPAPW CSSPAVPRASACTWPYVWLQIHPRASKV*IGRDR EGEKGGALGGQKGSQIFLLSQNLQCMPR*GT*K HRAGCGRRPGPWHALRDPWRRCSWT*GTQNPWP LPGNA*LRAAWTCWVSLLEAYGLLGAFSALR*NQ HVPRPREHEGTGRAEGDAEAGWSGLLSPQCVTQ AWACWGRWRRWGRTPWPLCWT*M**GLCGCCRP SCQT*RGAVRDACW*PGAWED*WVSGREWPRQLQ ILCVRS*ALKAGSAGGVEWGAVSLEGHRLPGDD PLAAAPQEPRLPT*GLPFNDVYCASKFALEGLC ESLAVLLLPFGVQ*VNTPVPRTLLTLT*RRRAPC PAGAALGRSPWPSLPGSH*LCARHLGWAHGTHC ATC*PSC*SVGIVMGKLQPREVR*LAQGHTAAR DPARFES*HQGSLVASDGFGRAAPAGTRVSNVLV IPSALSASLPSAAVLLAVGGRDVVGAGTGVGAG TGAWLASAPSHSLLSGQQLEPDRVRPSAHRLHG EGVGQPRGGAGPHGHPHLPPLLPIPRPQQASLSR GGAEP*GGGGGERRAGLQEWGRCVLRRAAVATA LLPPPQVFLTALRAPKPTLRYFTTERFLPLLRM RLDDPSGSNYVTAMHREVFGDVPAKAEAGAEAGG GAGPGAEDEAGRGAVGDPELGDPPAAPQ*RLPQ PLSPAPFFVPWVCVVPGDGAAVAAVGG*LR*IA LASFTSAARRDGCRL*CQRFGRRRQEDRSSPGVG DQPEQHSETPISTKIKKI

Sequence HSD17B1.gAug05

AGTTAGGTGGGACTTGGGTTGGGGTAGAGTCAGGTTGTGACCTGCACTCCATTAGCCATGAGACCTCAGGCAAGTTCCTTGCTTTCTCTGAGTGCTTTCC TTTCCTTTCCCTTTTCTTTTCTTTTTTGTTTTTCTTTTGTTTTGAGACGGAGTTTCACTCTTGTTGCCCAGCCTGGAGTGCAATAGCGCGGTCTCAGTTC ACCACAACCTCTGCCTCCCGGGTTCAAGTGTAGCTAGGATTGCAGGCATGTGCCACCACGCCCAGCTAATTTTGTATTTTTAGTAGAGACGAGGTTTCTC CATGTTGGTCAGACTGGTCTTGGACTCCCGACCTCAGGTGATCCGCCCGCCCTGGCCTCTCAGAGTGCTGGGATTACAGGCATGACCCACCCCATCCGGC CCTCTGAGTCTTTCTTATCTGTAAAATGGGTATAATAATACCTATCTAATCGGTTTTAGTAATGGTGGAGATAATGCCTGAAAGTGCTAGTATAGAGGTT TAATACCCAGCAAGGACTGTTATTAGAATGAATAGTAATATGACTACTGTCACATTTTGCAAATGTGTAAAGAAGAAAGAGGGCTAAGTGAATCAAAGGG AGACAGCCCCACTCACCCTGTTCTGCCCCAGAGGACTGAGCGATCCCCACCATCTGGGAAGCTGCCTCACCAGGAGGTCCAGCTGGGGCTACAGGACTGG CTACTTTGCTACAATGGCCCATCTTTCCTGAGCCCAGTGGAGGGTCCCAGGGGGGCAGAAGTCATTGATAGGGGCCAGTAGGGTTGTAGAGCCACTGTCT GAACTTCTGTGGAGGTCTGGTGCAGGGGAGGTGTGAACAGATAGGAGGCTAGGTGAGGATGCAGCAGAGGAAGGGGGCAGGAGTGCCCCAGGAGGGGGCG GTACCGAGGCAGGGATTCAGCTGGGTCTGAGAGGGAGGGAAGGCTGAGGGGGATGGCCCTTTGGAGTGGGCAGGGACATGACCACAGAAAGCCTGGGAAG TGGAAACAGACAGGAGCCTGCTGGAGCTCTTGGAGCTTTGTGTGGGGCACTAGGGGAAGGAGGCAGGCACTCCACCCTGACCTGCCCCTACCTCTGGATG AGGGTCTTCTCTGCCTGTTGGATGATGTGCTGCCCCTGCTCTTGGAGGAAGAGGCCTCCCAGCCACCCCCTCCTGCCAGTCGCCTTGCCCTGCTGACCCA GACAGATCTGCTATCCCTACAGCATCCAGGAAGGCCACAGGGAGGGGGCCCAGGTGGCATGGGTTTCTGAGGCCTGGGATCTGCTCTCAGTCCCGCTCTC GCCAACCCCCTTTGCCCCTCTGTGACAGTGTGTGTACATATGCGTGCATGTGTGTACCTGTGTGGTGTGGCTCACAAGGTCACCCCTGGGGGTGGGATAA TAAGAGGTAAAGTGTGACCCCCTCTCTTCCGTACATTCATTTTTTCAAGCTTGTGAATATTCACATTGTTAATATAATCTTCTGCAGTCCCATGACTTCG AATAGCACTTAGATGCCGATGACCCCCCCACCAAAGAAACCCCACTGTCTCCCACAGACTCCAGGTCTGTATCTCCAACTGCCTACTCAATACAACACGT CCACCTGATACAATGAACTTAATGTGTCTAAACCCCAATTCCACATTTCCAGTCCTCCTACCGGCTCCTCCCCCAGTCTTCTCTACTTTACTATATGACA ACTCCATTTTTCCCAATACTGGTTTTAAAAACCCTGGAGTAGTCCTTGACTCCAGTCTCTCTCACCTTTCAACCAATTCAGCAGCAAATACTGACAGCTC TACCTTTGCTCATCAAAGCATATCCCCAAATCACCCTATTCCAGACTGCACACTGTCATCTCTCCCTTCAATTACAGCAGTAGCCTCCTAACTAGTTTTC TTGATTCCACTCCTGCCCATCAGCAGTGAAATTACCCCAGAGCAGTTAAAATGATCTTTTAGGATGGGCACAGTGGCTCACGCCTGTAATACCAGCACTT TGGGAGGTGGAGGCTGGCTGATCCCCTTAGGTTGGGAGTTCAAGACCATCCTCACCAACAGGGAGAAACCCCATCTCTACTAAAAATACAAAAATTAGCC GGATGTGGTGGGACGTGTCTGTAATCCCAACTACTTGGGAGGCTGAGACAGGAGAACTGCTTGAACCCGGGAGGTGGAGGTTGCAGTGAGCCAGAATCGT GCCACAGCACTCCAGCCTGGGCAAGAAGCGCGAAACTCCGTCTCAAAATAAAATAATCTTCTAAAAATGACAGGGCCAGGTGGGGTGGCACTTTTTTATA ATCCGAGCACTTTGGGAGGCTGAGGTGGGCAGATCGCTTGACATCAGGGGTTTGAGACCAGCCTGGCCAACATGGTGAAACTCCGTCTCTACTAAAAATA CTAAAAATTAGCTGGGCGTGGTGGCGGGTGCCTGTAATCCCAGCTACTCGGGAGGCTGATGCAGGAGAATCGCCTGAACCAGGCAGATGCAGGATGCAGT GAGCCAAGATTAAGACACTGCACTCCAACCTAAGCAATACTTTGTCTCAAAAATAAAAAAAAGCCTGGGAAACAAAGTGAGACCCCGTCTCTACAAAAAA GTCAAAAAATTAGCTGGGTATGGTGGCAGTGATGGCACACACCTGTAGTCCCGGCTACTTGGGAAGCTTTTTAATATTTTTTGCAGAGACCGGGTCTCAC TCTGTTACCTGGCCTGGTCTTGAACTCCTGGGCTCCAACAATCCCCTTTCCTGGGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACCGTGCCCAGC CTCAAAGCATATTTTAAAGGATAGAAATAAACAGCCATATGAAGAGATACAGACAGGGCGGTCTGGAAGGGTCCAGAGCAGGAGCTTCTATCTCCATAGA GTTGGGGTTACGTCACCCTCTGGGCACATTCTGTCAGCCTCCACACGTTCAGCCCTCAGAAGCTCCCGAACCCTGTCCTTTGGGCCTTTTATGGAGAACT CCATTGGCTGTCCATGACTGAAGCATGGACAACTGTGATAATGTGATTGGGCAAAAAGGGTCTGATCTAAGCCCAGCAAGGCCAGTCCAGATTCTTTGGG CCTTTGTGCAGCATTCCTTTCTCCAGGGTATGGGGCAAGGACCCACTCTGGAATGAGGATCCTACAACCCACAATCAGATTAGAGTCCTGCCTTGGGCAG CTGAAAAGAGGACAGGAGAAGGTCAGAGAGACGAAAGGCTGTTTTTTGAGGCCTGAGGCACCCCAACATGACAACGTAAGACTGTAACCATGGTCATGTG AGTTATGAGCTAGGAACCCTGGACGAAACCAGCACATATACAATCATCTCCCACCTCCCAACGCCTTTACTTTCACAGCCTCTGCAGCAAACTGCGGTCA CTATAATCGCTCCTGTGGCACAGAGGCATACCCAGGGGAATCTGCCCAGGGGGCCACTCTGTGCCCACGTGGGAACCCACACCTGCTTGTAAAGCCTCCC CTCCCTCTGACCAGCAACCAGGACAGTTTGTTGTTCCAAGCAGTGGGCTCATGTCTGTTTTGGCTCAGAACAGGGTGGGGAGAGCGGGCCAGGGACCCGC AGGAAGGCTTATCCTTGAGATTGCGTGGGAGACACAACAAGGGGTGGGGGCCCGCAGGCGGGGCGGGGCGAAGCAGGTGATATCAAGCCCAGAGCCCCAG CCTCTCCCCACAGTCTCACCATGGCCCGCACCGTGGTGCTCATCACCGGCTGTTCCTCGGGCATCGGCCTGCACTTGGCCGTACGTCTGGCTTCAGATCC ATCCCAGAGCTTCAAAGTGTATGCCACGTTGAGGGACCTGAAAACACAGGGCCGGCTGTGGGAGGCGGCCCGGGCCCTGGCATGCCCTCCGGGATCCCTG GAGACGTTGCAGCTGGACGTAAGGGACTCAAAATCCGTGGCCGCTGCCCGGGAACGCGTGACTGAGGGCCGCGTGGACGTGCTGGGTGAGCCTCCTGGAA GCATATGGGCTCCTAGGAGCCTTCTCCGCCCTGCGTTGAAACCAACATGTTCCCAGGCCCAGGGAGCACGAGGGGACAGGCCGTGCTGAGGGTGATGCTG AGGCGGGCTGGTCGGGCCTCTTGTCTCCGCAGTGTGTAACGCAGGCCTGGGCCTGCTGGGGCCGCTGGAGGCGCTGGGGGAGGACGCCGTGGCCTCTGTG CTGGACGTGAATGTAGTAGGGACTGTGCGGATGCTGCAGGCCTTCCTGCCAGACATGAAGAGGCGCGGTTCGGGACGCGTGTTGGTGACCGGGAGCGTGG GAGGATTGATGGGTGAGTGGTAGGGAGTGGCCTCGGCAGCTCCAGATTCTTTGTGTGCGGAGCTGAGCCTTGAAGGCAGGTTCCGCGGGGGGGGTGGAGT GGGGTGCCGTCAGCTTGGAGGGGCACCGTCTGCCCGGGGATGACCCCCTGGCCGCTGCGCCTCAGGAACCTCGTCTCCCCACCTAAGGGCTGCCTTTCAA TGACGTTTATTGCGCCAGCAAGTTCGCGCTCGAAGGCTTATGCGAGAGTCTGGCGGTTCTGCTGCTGCCCTTTGGGGTCCAGTGAGTCAACACCCCCGTT CCCCGAACCCTCTTAACTCTGACCTAGAGACGCCGAGCACCCTGTCCTGCGGGAGCCGCTCTGGGGCGATCTCCCTGGCCCTCTCTGCCCGGCTCACATT AGCTGTGTGCCAGGCACTTGGGCTGGGCGCATGGCACGCATTGTGCCACCTGCTGACCTAGCTGCTGAAGTGTTGGTATTGTTATGGGGAAGCTCCAGCC CAGAGAGGTTAGGTGACTGGCCCAAGGTCACACAGCGGCCAGGGACCCCGCTAGATTCGAATCCTGACACCAGGGCTCCCTTGTAGCCTCAGATGGATTT GGGAGGGCTGCTCCGGCAGGAACCCGCGTTTCAAATGTTCTGGTTATCCCCAGCGCCCTTTCCGCCTCACTTCCCAGCGCAGCGGTGCTGCTCGCGGTCG GGGGCCGGGACGTGGTTGGGGCTGGGACTGGGGTTGGGGCTGGGACTGGGGCCTGGCTGGCGTCCGCCCCCTCCCACTCGTTGCTCTCCGGCCAGCAGCT TGAGCCTGATCGAGTGCGGCCCAGTGCACACCGCCTTCATGGAGAAGGTGTTGGGCAGCCCAGAGGAGGTGCTGGACCGCACGGACATCCACACCTTCCA CCGCTTCTACCAATACCTCGCCCACAGCAAGCAAGTCTTTCGCGAGGCGGCGCAGAACCCTGAGGAGGTGGCGGAGGTGAGCGCCGGGCTGGACTCCAGG AGTGGGGGCGGTGCGTCCTCCGGCGCGCAGCGGTGGCCACAGCTCTCCTCCCGCCGCCGCAGGTCTTCCTCACCGCTTTGCGCGCCCCGAAGCCGACCCT GCGCTACTTCACCACCGAGCGCTTCCTGCCCCTGCTGCGGATGCGCCTGGACGACCCCAGCGGCTCCAACTACGTCACCGCCATGCACCGGGAAGTGTTC GGCGACGTTCCGGCAAAGGCCGAGGCTGGGGCCGAGGCTGGGGGCGGGGCCGGGCCTGGGGCAGAGGACGAGGCCGGGCGCGGTGCGGTGGGGGACCCTG AGCTCGGCGATCCTCCGGCCGCCCCGCAGTAAAGGCTTCCTCAGCCGCTGTCTCCCGCGCCCTTCTTTGTCCCCTGGGTCTGTGTGGTCCCTGGGGATGG GGCGGCGGTAGCAGCTGTGGGTGGCTAATTAAGATAGATCGCGTTAGCCAGTTTTACCAGCGCAGCTAGGCGCGATGGCTGTCGCCTGTAATGCCAGCGC TTTGGGAGGCGGAGGCAGGAGGATCGCTCAAGCCCCGGAGTTGGAGACCAGCCAGAGCAACACAGTGAGACCCCCATCTCTACAAAAATAAAGAAAATTT

HSD17B1.F4 (forward primer)
HSD17B1.R4 (reverse primer)
Intron retention

Frame 0

S*VGLGLG*SQVVTCTPLAMRPQASSLLSLSAF LSFPFSFLFCFSFVLRRSFTLVAQPGVQ*RGLSS PQPLPPGFKCS*DCRHVPPRPANFVFLVETRFL HVGQTGLGLPTSGDPPALASQSAGITGMTHPIR PSESFLSVKWV**YLSNRF**WWR*CLKVLV*RF NTQQGLLLE*IVI*LLSHFANV*RRKRAK*IKG RQPHSPCSAPED*AIPTIWEAASPGGPAGATGL ATLLQWPIFPEPSGGSQGGRSH**GPVGL*SHCL NFCGGLVQGRCEQIGG*VRMQQRKGAGVPQEGA VPRQGFSWV*EGGKAEGDGPLEWAGT*PQKAWE VETDRSLLELLELCVGH*GKEAGTPP*PAPTSG* GSSLPVG*CAAPALGGRGLPATPSCQSPCPADP DRSAIPTASRKATGRGPRWHGFLRPGICSQSRS RQPPLPLCDSVCTYACMCVPVWCGSQGHPWGWDN KR*SVTPSLPYIHFFKLVNIHIVNIIFCSPMTS NST*MPMTPPPKKPHCLPQTPGLYLQLPTQYNT ST*YNELNVSKPQFHISSPPTGSSPSLLYFTI*Q LHFSQYWF*KPWSSP*LQSLSPFNQFSSKY*QL YLCSSKHIPKSPYSRLHTVISPFNYSSSLLTSF LDSTPAHQQ*NYPRAVKMIF*DGHSGSRL*YQHF GRWRLADPLRLGVQDHPHQQGETPSLLKIQKLA GCGGTCL*SQLLGRLRQENCLNPGGGGCSEPES CHSTPAWARSAKLRLKIK*SSKNDRARWGGTFL* SEHFGRLRWADRLTSGV*DQPGQHGETPSLLKI LKISWAWWRVPVIPATREADAGESPEPGRCRMQ *AKIKTLHSNLSNTLSQK*KKAWETK*DPVSTKK SKN*LGMVAVMAHTCSPGYLGSFLIFFAETGSH SVTWPGLELLGSNNPLSWASQSAGITGMSHRAQ PQSIF*RIEINSHMKRYRQGGLEGSRAGASISIE LGLRHPLGTFCQPPHVQPSEAPEPCPLGLLWRT PLAVHD*SMDNCDNVIGQKGSDLSPARPVQILW AFVQHSFLQGMGQGPTLE*GSYNPQSD*SPALGS *KEDRRRSERRKAVF*GLRHPNMTT*DCNHGHV SYELGTLDETSTYTIISHLPTPLLSQPLQQTAV TIIAPVAQRHTQGNLPRGPLCAHVGTHTCL*SLP SL*PATRTVCCSKQWAHVCFGSEQGGESGPGTR RKAYP*DCVGDTTRGGGPQAGRGEAGDIKPRAP ASPHSLTMARTVVLITGCSSGIGLHLAVRLASDP SQSFKVYATLRDLKTQGRLWEAARALACPPGSL ETLQLDVRDSKSVAAARERVTEGRVDVLGEPPG SIWAPRSLLRPALKPTCSQAQGARGDRPC*G*C* GGLVGPLVSAVCNAGLGLLGPLEALGEDAVASV LDVNVVGTVRMLQAFLPDMKRRGSGRVLVTGSV GGLMGEW*GVASAAPDSLCAELSLEGRFRGGGGV GCRQLGGAPSARG*PPGRCASGTSSPHLRAAFQ *RLLRQQVRARRLMRESGGSAAALWGPVSQHPR SPNPLNSDLETPSTLSCGSRSGAISLALSARLTL AVCQALGLGAWHALCHLLT*LLKCWYCYGEAPA QRG*VTGPRSHSGQGPR*IRILTPGLPCSLRWI WEGCSGRNPRFKCSGYPQRPFRLTSQRSGAARGR GPGRGWGWDWGWGWDWGLAGVRPLPLVALRPAA *A*SSAAQCTPPSWRRCWAAQRRCWTARTSTPS TASTNTSPTASKSFARRRRTLRRWRR*APGWTPG VGAVRPPARSGGHSSPPAAAGLPHRFARPEADP ALLHHRALPAPAADAPGRPQRLQLRHRHAPGSV RRRSGKGRGWGRGWGRGRAWGRGRGRARCGGGP* ARRSSGRPAVKASSAAVSRALLCPLGLCGPWGW GGGSSCGWLIKIDRVSQFYQRS*ARWLSPVMPA LWEAEAGGSLKPRSWRPARATQ*DPHLYKNKEN

Frame 1

VRWDLGWGRVRL*PALH*P*DLRQVPCFL*VLS FPFPFLFFFVFLLF*DGVSLLLPSLECNSAVSV HHNLCLPGSSVARIAGMCHHAQLILYF**RRGFS MLVRLVLDSRPQVIRPPWPLRVLGLQA*PTPSG PLSLSYL*NGYNNTYLIGFSNGGDNA*KC*YRG LIPSKDCY*NE**YDYCHILQMCKEERGLSESKG DSPTHPVLPQRTERSPPSGKLPHQEVQLGLQDW LLCYNGPSFLSPVEGPRGAEVIDRGQ*GCRATV *TSVEVWCRGGVNR*EAR*GCSRGRGQECPRRGR YRGRDSAGSEREGRLRGMALWSGQGHDHRKPGK WKQTGACWSSWSFVWGTRGRRQALHPDLPLPLD EGLLCLLDDVLPLLLEEEASQPPPPASRLALLTQ TDLLSLQHPGRPQGGGPGGMGF*GLGSALSPAL ANPLCPSVTVCVHMRACVYLCGVAHKVTPGGGI IRGKV*PPLFRTFIFSSL*IFTLLI*SSAVP*LR IALRCR*PPHQRNPTVSHRLQVCISNCLLNTTR PPDTMNLMCLNPNSTFPVLLPAPPPVFSTLLYD NSIFPNTGFKNPGVVLDSSLSHLSTNSAANTDSS TFAHQSISPNHPIPDCTLSSLPSITAVAS*LVF LIPLLPISSEITPEQLK*SFRMGTVAHACNTST LGGGGWLIPLGWEFKTILTNREKPHLY*KYKN*P DVVGRVCNPNYLGG*DRRTA*TREVEVAVSQNR ATALQPGQEARNSVSK*NNLLKMTGPGGVALFY NPSTLGG*GGQIA*HQGFETSLANMVKLRLY*KY *KLAGRGGGCL*SQLLGRLMQENRLNQADAGCS EPRLRHCTPT*AILCLKNKKKPGKQSETPSLQK SQKISWVWWQ*WHTPVVPATWEAF*YFLQRPGLT LLPGLVLNSWAPTIPFPGPPKVLGLQA*ATVPS LKAYFKG*K*TAI*RDTDRAVWKGPEQELLSP* SWGYVTLWAHSVSLHTFSPQKLPNPVLWAFYGEL HWLSMTEAWTTVIM*LGKKGLI*AQQGQSRFFG PLCSIPFSRVWGKDPLWNEDPTTHNQIRVLPWA AEKRTGEGQRDERLFFEA*GTPT*QRKTVTMVM* VMS*EPWTKPAHIQSSPTSQRLYFHSLCSKLRS L*SLLWHRGIPRGICPGGHSVPTWEPTPACKAS PPSDQQPGQFVVPSSGLMSVLAQNRVGRAGQGPA GRLILEIAWETQQGVGARRRGGAKQVISSPEPQ PLPTVSPWPAPWCSSPAVPRASACTWPYVWLQI HPRASKCMPR*GT*KHRAGCGRRPGPWHALRDPW RRCSWT*GTQNPWPLPGNA*LRAAWTCWVSLLE AYGLLGAFSALR*NQHVPRPREHEGTGRAEGDA EAGWSGLLSPQCVTQAWACWGRWRRWGRTPWPLC WT*M**GLCGCCRPSCQT*RGAVRDACW*PGAW ED*WVSGREWPRQLQILCVRS*ALKAGSAGGVE WGAVSLEGHRLPGDDPLAAAPQEPRLPT*GLPFN DVYCASKFALEGLCESLAVLLLPFGVQ*VNTPV PRTLLTLT*RRRAPCPAGAALGRSPWPSLPGSH *LCARHLGWAHGTHCATC*PSC*SVGIVMGKLQP REVR*LAQGHTAARDPARFES*HQGSLVASDGF GRAAPAGTRVSNVLVIPSALSASLPSAAVLLAV GGRDVVGAGTGVGAGTGAWLASAPSHSLLSGQQL EPDRVRPSAHRLHGEGVGQPRGGAGPHGHPHLP PLLPIPRPQQASLSRGGAEP*GGGGGERRAGLQ EWGRCVLRRAAVATALLPPPQVFLTALRAPKPTL RYFTTERFLPLLRMRLDDPSGSNYVTAMHREVF GDVPAKAEAGAEAGGGAGPGAEDEAGRGAVGDP ELGDPPAAPQ*RLPQPLSPAPFFVPWVCVVPGDG AAVAAVGG*LR*IALASFTSAARRDGCRL*CQR FGRRRQEDRSSPGVGDQPEQHSETPISTKIKKI

Frame 2

LGGTWVGVESGCDLHSISHETSGKFLAFSECFP FLSLFFSFLFFFCFETEFHSCCPAWSAIARSQF TTTSASRVQV*LGLQACATTPS*FCIFSRDEVS PCWSDWSWTPDLR*SARPGLSECWDYRHDPPHPA L*VFLICKMGIIIPI*SVLVMVEIMPESASIEV *YPARTVIRMNSNMTTVTFCKCVKKKEG*VNQR ETAPLTLFCPRGLSDPHHLGSCLTRRSSWGYRTG YFATMAHLS*AQWRVPGGQKSLIGASRVVEPLS ELLWRSGAGEV*TDRRLGEDAAEEGGRSAPGGG GTEAGIQLGLRGREG*GGWPFGVGRDMTTESLGS GNRQEPAGALGALCGALGEGGRHSTLTCPYLWM RVFSACWMMCCPCSWRKRPPSHPLLPVALPC*P RQICYPYSIQEGHREGAQVAWVSEAWDLLSVPLS PTPFAPL*QCVYICVHVCTCVVWLTRSPLGVG* *EVKCDPLSSVHSFFQACEYSHC*YNLLQSHDF E*HLDADDPPTKETPLSPTDSRSVSPTAYSIQHV HLIQ*T*CV*TPIPHFQSSYRLLPQSSLLYYMT TPFFPILVLKTLE*SLTPVSLTFQPIQQQILTA LPLLIKAYPQITLFQTAHCHLSLQLQQ*PPN*FS *FHSCPSAVKLPQSS*NDLLGWAQWLTPVIPAL WEVEAG*SP*VGSSRPSSPTGRNPISTKNTKIS RMWWDVSVIPTTWEAETGELLEPGRWRLQ*ARIV PQHSSLGKKRETPSQNKIIF*K*QGQVGWHFFI IRALWEAEVGRSLDIRGLRPAWPTW*NSVSTKN TKN*LGVVAGACNPSYSGG*CRRIA*TRQMQDAV SQD*DTALQPKQYFVSKIKKSLGNKVRPRLYKK VKKLAGYGGSDGTHL*SRLLGKLFNIFCRDRVS LCYLAWS*TPGLQQSPFLGLPKCWDYRHEPPCPA SKHILKDRNKQPYEEIQTGRSGRVQSRSFYLHR VGVTSPSGHILSASTRSALRSSRTLSFGPFMEN SIGCP*LKHGQL**CDWAKRV*SKPSKASPDSLG LCAAFLSPGYGARTHSGMRILQPTIRLESCLGQ LKRGQEKVRETKGCFLRPEAPQHDNVRL*PWSC EL*ARNPGRNQHIYNHLPPPNAFTFTASAANCGH YNRSCGTEAYPGESAQGATLCPRGNPHLLVKPP LPLTSNQDSLLFQAVGSCLFWLRTGWGERARDP QEGLSLRLRGRHNKGWGPAGGAGRSR*YQAQSPS LSPQSHHGPHRGAHHRLFLGHRPALGRTSGFRS IPELQSVCHVEGPENTGPAVGGGPGPGMPSGIP GDVAAGRKGLKIRGRCPGTRD*GPRGRAG*ASWK HMGS*EPSPPCVETNMFPGPGSTRGQAVLRVML RRAGRASCLRSV*RRPGPAGAAGGAGGGRRGLC AGRECSRDCADAAGLPARHEEARFGTRVGDRERG RIDG*VVGSGLGSSRFFVCGAEP*RQVPRGGWS GVPSAWRGTVCPGMTPWPLRLRNLVSPPKGCLS MTFIAPASSRSKAYARVWRFCCCPLGSSESTPPF PEPS*L*PRDAEHPVLREPLWGDLPGPLCPAHI SCVPGTWAGRMARIVPPADLAAEVLVLLWGSSS PERLGDWPKVTQRPGTPLDSNPDTRAPL*PQMDL GGLLRQEPAFQMFWLSPAPFPPHFPAQRCCSRS GAGTWLGLGLGLGLGLGPGWRPPPPTRCSPASS LSLIECGPVHTAFMEKVLGSPEEVLDRTDIHTFH RFYQYLAHSKQVFREAAQNPEEVAEVSAGLDSR SGGGASSGAQRWPQLSSRRRRSSSPLCAPRSRP CATSPPSASCPCCGCAWTTPAAPTTSPPCTGKCS ATFRQRPRLGPRLGAGPGLGQRTRPGAVRWGTL SSAILRPPRSKGFLSRCLPRPSLSPGSVWSLGM GRR*QLWVAN*DRSR*PVLPAQLGAMAVACNASA LGGGGRRIAQAPELETSQSNTVRPPSLQK*RKF

Sequence HSD17B1.eAug05

AGTTAGGTGGGACTTGGGTTGGGGTAGAGTCAGGTTGTGACCTGCACTCCATTAGCCATGAGACCTCAGGCAAGTTCCTTGCTTTCTCTGAGTGCTTTCC TTTCCTTTCCCTTTTCTTTTCTTTTTTGTTTTTCTTTTGTTTTGAGACGGAGTTTCACTCTTGTTGCCCAGCCTGGAGTGCAATAGCGCGGTCTCAGTTC ACCACAACCTCTGCCTCCCGGGTTCAAGTGTAGCTAGGATTGCAGGCATGTGCCACCACGCCCAGCTAATTTTGTATTTTTAGTAGAGACGAGGTTTCTC CATGTTGGTCAGACTGGTCTTGGACTCCCGACCTCAGGTGATCCGCCCGCCCTGGCCTCTCAGAGTGCTGGGATTACAGGCATGACCCACCCCATCCGGC CCTCTGAGTCTTTCTTATCTGTAAAATGGGTATAATAATACCTATCTAATCGGTTTTAGTAATGGTGGAGATAATGCCTGAAAGTGCTAGTATAGAGGTT TAATACCCAGCAAGGACTGTTATTAGAATGAATAGTAATATGACTACTGTCACATTTTGCAAATGTGTAAAGAAGAAAGAGGGCTAAGTGAATCAAAGGG AGACAGCCCCACTCACCCTGTTCTGCCCCAGAGGACTGAGCGATCCCCACCATCTGGGAAGCTGCCTCACCAGGAGGTCCAGCTGGGGCTACAGGACTGG CTACTTTGCTACAATGGCCCATCTTTCCTGAGCCCAGTGGAGGGTCCCAGGGGGGCAGAAGTCATTGATAGGGGCCAGTAGGGTTGTAGAGCCACTGTCT GAACTTCTGTGGAGGTCTGGTGCAGGGGAGGTGTGAACAGATAGGAGGCTAGGTGAGGATGCAGCAGAGGAAGGGGGCAGGAGTGCCCCAGGAGGGGGCG GTACCGAGGCAGGGATTCAGCTGGGTCTGAGAGGGAGGGAAGGCTGAGGGGGATGGCCCTTTGGAGTGGGCAGGGACATGACCACAGAAAGCCTGGGAAG TGGAAACAGACAGGAGCCTGCTGGAGCTCTTGGAGCTTTGTGTGGGGCACTAGGGGAAGGAGGCAGGCACTCCACCCTGACCTGCCCCTACCTCTGGATG AGGGTCTTCTCTGCCTGTTGGATGATGTGCTGCCCCTGCTCTTGGAGGAAGAGGCCTCCCAGCCACCCCCTCCTGCCAGTCGCCTTGCCCTGCTGACCCA GACAGATCTGCTATCCCTACAGCATCCAGGAAGGCCACAGGGAGGGGGCCCAGGTGGCATGGGTTTCTGAGGCCTGGGATCTGCTCTCAGTCCCGCTCTC GCCAACCCCCTTTGCCCCTCTGTGACAGTGTGTGTACATATGCGTGCATGTGTGTACCTGTGTGGTGTGGCTCACAAGGTCACCCCTGGGGGTGGGATAA TAAGAGGTAAAGTGTGACCCCCTCTCTTCCGTACATTCATTTTTTCAAGCTTGTGAATATTCACATTGTTAATATAATCTTCTGCAGTCCCATGACTTCG AATAGCACTTAGATGCCGATGACCCCCCCACCAAAGAAACCCCACTGTCTCCCACAGACTCCAGGTCTGTATCTCCAACTGCCTACTCAATACAACACGT CCACCTGATACAATGAACTTAATGTGTCTAAACCCCAATTCCACATTTCCAGTCCTCCTACCGGCTCCTCCCCCAGTCTTCTCTACTTTACTATATGACA ACTCCATTTTTCCCAATACTGGTTTTAAAAACCCTGGAGTAGTCCTTGACTCCAGTCTCTCTCACCTTTCAACCAATTCAGCAGCAAATACTGACAGCTC TACCTTTGCTCATCAAAGCATATCCCCAAATCACCCTATTCCAGACTGCACACTGTCATCTCTCCCTTCAATTACAGCAGTAGCCTCCTAACTAGTTTTC TTGATTCCACTCCTGCCCATCAGCAGTGAAATTACCCCAGAGCAGTTAAAATGATCTTTTAGGATGGGCACAGTGGCTCACGCCTGTAATACCAGCACTT TGGGAGGTGGAGGCTGGCTGATCCCCTTAGGTTGGGAGTTCAAGACCATCCTCACCAACAGGGAGAAACCCCATCTCTACTAAAAATACAAAAATTAGCC GGATGTGGTGGGACGTGTCTGTAATCCCAACTACTTGGGAGGCTGAGACAGGAGAACTGCTTGAACCCGGGAGGTGGAGGTTGCAGTGAGCCAGAATCGT GCCACAGCACTCCAGCCTGGGCAAGAAGCGCGAAACTCCGTCTCAAAATAAAATAATCTTCTAAAAATGACAGGGCCAGGTGGGGTGGCACTTTTTTATA ATCCGAGCACTTTGGGAGGCTGAGGTGGGCAGATCGCTTGACATCAGGGGTTTGAGACCAGCCTGGCCAACATGGTGAAACTCCGTCTCTACTAAAAATA CTAAAAATTAGCTGGGCGTGGTGGCGGGTGCCTGTAATCCCAGCTACTCGGGAGGCTGATGCAGGAGAATCGCCTGAACCAGGCAGATGCAGGATGCAGT GAGCCAAGATTAAGACACTGCACTCCAACCTAAGCAATACTTTGTCTCAAAAATAAAAAAAAGCCTGGGAAACAAAGTGAGACCCCGTCTCTACAAAAAA GTCAAAAAATTAGCTGGGTATGGTGGCAGTGATGGCACACACCTGTAGTCCCGGCTACTTGGGAAGCTTTTTAATATTTTTTGCAGAGACCGGGTCTCAC TCTGTTACCTGGCCTGGTCTTGAACTCCTGGGCTCCAACAATCCCCTTTCCTGGGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACCGTGCCCAGC CTCAAAGCATATTTTAAAGGATAGAAATAAACAGCCATATGAAGAGATACAGACAGGGCGGTCTGGAAGGGTCCAGAGCAGGAGCTTCTATCTCCATAGA GTTGGGGTTACGTCACCCTCTGGGCACATTCTGTCAGCCTCCACACGTTCAGCCCTCAGAAGCTCCCGAACCCTGTCCTTTGGGCCTTTTATGGAGAACT CCATTGGCTGTCCATGACTGAAGCATGGACAACTGTGATAATGTGATTGGGCAAAAAGGGTCTGATCTAAGCCCAGCAAGGCCAGTCCAGATTCTTTGGG CCTTTGTGCAGCATTCCTTTCTCCAGGGTATGGGGCAAGGACCCACTCTGGAATGAGGATCCTACAACCCACAATCAGATTAGAGTCCTGCCTTGGGCAG CTGAAAAGAGGACAGGAGAAGGTCAGAGAGACGAAAGGCTGTTTTTTGAGGCCTGAGGCACCCCAACATGACAACGTAAGACTGTAACCATGGTCATGTG AGTTATGAGCTAGGAACCCTGGACGAAACCAGCACATATACAATCATCTCCCACCTCCCAACGCCTTTACTTTCACAGCCTCTGCAGCAAACTGCGGTCA CTATAATCGCTCCTGTGGCACAGAGGCATACCCAGGGGAATCTGCCCAGGGGGCCACTCTGTGCCCACGTGGGAACCCACACCTGCTTGTAAAGCCTCCC CTCCCTCTGACCAGCAACCAGGACAGTTTGTTGTTCCAAGCAGTGGGCTCATGTCTGTTTTGGCTCAGAACAGGGTGGGGAGAGCGGGCCAGGGACCCGC AGGAAGGCTTATCCTTGAGATTGCGTGGGAGACACAACAAGGGGTGGGGGCCCGCAGGCGGGGCGGGGCGAAGCAGGTGATATCAAGCCCAGAGCCCCAG CCTCTCCCCACAGTCTCACCATGGCCCGCACCGTGGTGCTCATCACCGGCTGTTCCTCGGGCATCGGCCTGCACTTGGCCGTACGTCTGGCTTCAGATCC ATCCCAGAGCTTCAAAGTGTATGCCACGTTGAGGGACCTGAAAACACAGGGCCGGCTGTGGGAGGCGGCCCGGGCCCTGGCATGCCCTCCGGGATCCCTG GAGACGTTGCAGCTGGACGTAAGGGACTCAAAATCCGTGGCCGCTGCCCGGGAACGCGTGACTGAGGGCCGCGTGGACGTGCTGGTGTGTAACGCAGGCC TGGGCCTGCTGGGGCCGCTGGAGGCGCTGGGGGAGGACGCCGTGGCCTCTGTGCTGGACGTGAATGTAGTAGGGACTGTGCGGATGCTGCAGGCCTTCCT GCCAGACATGAAGAGGCGCGGTTCGGGACGCGTGTTGGTGACCGGGAGCGTGGGAGGATTGATGGGTGAGTGGTAGGGAGTGGCCTCGGCAGCTCCAGAT TCTTTGTGTGCGGAGCTGAGCCTTGAAGGCAGGTTCCGCGGGGGGGGTGGAGTGGGGTGCCGTCAGCTTGGAGGGGCACCGTCTGCCCGGGGATGACCCC CTGGCCGCTGCGCCTCAGGAACCTCGTCTCCCCACCTAAGGGCTGCCTTTCAATGACGTTTATTGCGCCAGCAAGTTCGCGCTCGAAGGCTTATGCGAGA GTCTGGCGGTTCTGCTGCTGCCCTTTGGGGTCCAGTGAGTCAACACCCCCGTTCCCCGAACCCTCTTAACTCTGACCTAGAGACGCCGAGCACCCTGTCC TGCGGGAGCCGCTCTGGGGCGATCTCCCTGGCCCTCTCTGCCCGGCTCACATTAGCTGTGTGCCAGGCACTTGGGCTGGGCGCATGGCACGCATTGTGCC ACCTGCTGACCTAGCTGCTGAAGTGTTGGTATTGTTATGGGGAAGCTCCAGCCCAGAGAGGTTAGGTGACTGGCCCAAGGTCACACAGCGGCCAGGGACC CCGCTAGATTCGAATCCTGACACCAGGGCTCCCTTGTAGCCTCAGATGGATTTGGGAGGGCTGCTCCGGCAGGAACCCGCGTTTCAAATGTTCTGGTTAT CCCCAGCGCCCTTTCCGCCTCACTTCCCAGCGCAGCGGTGCTGCTCGCGGTCGGGGGCCGGGACGTGGTTGGGGCTGGGACTGGGGTTGGGGCTGGGACT GGGGCCTGGCTGGCGTCCGCCCCCTCCCACTCGTTGCTCTCCGGCCAGCAGCTTGAGCCTGATCGAGTGCGGCCCAGTGCACACCGCCTTCATGGAGAAG GTGTTGGGCAGCCCAGAGGAGGTGCTGGACCGCACGGACATCCACACCTTCCACCGCTTCTACCAATACCTCGCCCACAGCAAGCAAGTCTTTCGCGAGG CGGCGCAGAACCCTGAGGAGGTGGCGGAGGTGAGCGCCGGGCTGGACTCCAGGAGTGGGGGCGGTGCGTCCTCCGGCGCGCAGCGGTGGCCACAGCTCTC CTCCCGCCGCCGCAGGTCTTCCTCACCGCTTTGCGCGCCCCGAAGCCGACCCTGCGCTACTTCACCACCGAGCGCTTCCTGCCCCTGCTGCGGATGCGCC TGGACGACCCCAGCGGCTCCAACTACGTCACCGCCATGCACCGGGAAGTGTTCGGCGACGTTCCGGCAAAGGCCGAGGCTGGGGCCGAGGCTGGGGGCGG GGCCGGGCCTGGGGCAGAGGACGAGGCCGGGCGCGGTGCGGTGGGGGACCCTGAGCTCGGCGATCCTCCGGCCGCCCCGCAGTAAAGGCTTCCTCAGCCG CTGTCTCCCGCGCCCTTCTTTGTCCCCTGGGTCTGTGTGGTCCCTGGGGATGGGGCGGCGGTAGCAGCTGTGGGTGGCTAATTAAGATAGATCGCGTTAG CCAGTTTTACCAGCGCAGCTAGGCGCGATGGCTGTCGCCTGTAATGCCAGCGCTTTGGGAGGCGGAGGCAGGAGGATCGCTCAAGCCCCGGAGTTGGAGA CCAGCCAGAGCAACACAGTGAGACCCCCATCTCTACAAAAATAAAGAAAATTT

HSD17B1.F4 (forward primer)
HSD17B1.R4 (reverse primer)
Intron retention

Frame 0

S*VGLGLG*SQVVTCTPLAMRPQASSLLSLSAF LSFPFSFLFCFSFVLRRSFTLVAQPGVQ*RGLSS PQPLPPGFKCS*DCRHVPPRPANFVFLVETRFL HVGQTGLGLPTSGDPPALASQSAGITGMTHPIR PSESFLSVKWV**YLSNRF**WWR*CLKVLV*RF NTQQGLLLE*IVI*LLSHFANV*RRKRAK*IKG RQPHSPCSAPED*AIPTIWEAASPGGPAGATGL ATLLQWPIFPEPSGGSQGGRSH**GPVGL*SHCL NFCGGLVQGRCEQIGG*VRMQQRKGAGVPQEGA VPRQGFSWV*EGGKAEGDGPLEWAGT*PQKAWE VETDRSLLELLELCVGH*GKEAGTPP*PAPTSG* GSSLPVG*CAAPALGGRGLPATPSCQSPCPADP DRSAIPTASRKATGRGPRWHGFLRPGICSQSRS RQPPLPLCDSVCTYACMCVPVWCGSQGHPWGWDN KR*SVTPSLPYIHFFKLVNIHIVNIIFCSPMTS NST*MPMTPPPKKPHCLPQTPGLYLQLPTQYNT ST*YNELNVSKPQFHISSPPTGSSPSLLYFTI*Q LHFSQYWF*KPWSSP*LQSLSPFNQFSSKY*QL YLCSSKHIPKSPYSRLHTVISPFNYSSSLLTSF LDSTPAHQQ*NYPRAVKMIF*DGHSGSRL*YQHF GRWRLADPLRLGVQDHPHQQGETPSLLKIQKLA GCGGTCL*SQLLGRLRQENCLNPGGGGCSEPES CHSTPAWARSAKLRLKIK*SSKNDRARWGGTFL* SEHFGRLRWADRLTSGV*DQPGQHGETPSLLKI LKISWAWWRVPVIPATREADAGESPEPGRCRMQ *AKIKTLHSNLSNTLSQK*KKAWETK*DPVSTKK SKN*LGMVAVMAHTCSPGYLGSFLIFFAETGSH SVTWPGLELLGSNNPLSWASQSAGITGMSHRAQ PQSIF*RIEINSHMKRYRQGGLEGSRAGASISIE LGLRHPLGTFCQPPHVQPSEAPEPCPLGLLWRT PLAVHD*SMDNCDNVIGQKGSDLSPARPVQILW AFVQHSFLQGMGQGPTLE*GSYNPQSD*SPALGS *KEDRRRSERRKAVF*GLRHPNMTT*DCNHGHV SYELGTLDETSTYTIISHLPTPLLSQPLQQTAV TIIAPVAQRHTQGNLPRGPLCAHVGTHTCL*SLP SL*PATRTVCCSKQWAHVCFGSEQGGESGPGTR RKAYP*DCVGDTTRGGGPQAGRGEAGDIKPRAP ASPHSLTMARTVVLITGCSSGIGLHLAVRLASDP SQSFKVYATLRDLKTQGRLWEAARALACPPGSL ETLQLDVRDSKSVAAARERVTEGRVDVLVCNAG LGLLGPLEALGEDAVASVLDVNVVGTVRMLQAFL PDMKRRGSGRVLVTGSVGGLMGEW*GVASAAPD SLCAELSLEGRFRGGGGVGCRQLGGAPSARG*P PGRCASGTSSPHLRAAFQ*RLLRQQVRARRLMRE SGGSAAALWGPVSQHPRSPNPLNSDLETPSTLS CGSRSGAISLALSARLTLAVCQALGLGAWHALC HLLT*LLKCWYCYGEAPAQRG*VTGPRSHSGQGP R*IRILTPGLPCSLRWIWEGCSGRNPRFKCSGY PQRPFRLTSQRSGAARGRGPGRGWGWDWGWGWD WGLAGVRPLPLVALRPAA*A*SSAAQCTPPSWRR CWAAQRRCWTARTSTPSTASTNTSPTASKSFAR RRRTLRRWRR*APGWTPGVGAVRPPARSGGHSS PPAAAGLPHRFARPEADPALLHHRALPAPAADAP GRPQRLQLRHRHAPGSVRRRSGKGRGWGRGWGR GRAWGRGRGRARCGGGP*ARRSSGRPAVKASSA AVSRALLCPLGLCGPWGWGGGSSCGWLIKIDRVS QFYQRS*ARWLSPVMPALWEAEAGGSLKPRSWR PARATQ*DPHLYKNKEN

Frame 1

VRWDLGWGRVRL*PALH*P*DLRQVPCFL*VLS FPFPFLFFFVFLLF*DGVSLLLPSLECNSAVSV HHNLCLPGSSVARIAGMCHHAQLILYF**RRGFS MLVRLVLDSRPQVIRPPWPLRVLGLQA*PTPSG PLSLSYL*NGYNNTYLIGFSNGGDNA*KC*YRG LIPSKDCY*NE**YDYCHILQMCKEERGLSESKG DSPTHPVLPQRTERSPPSGKLPHQEVQLGLQDW LLCYNGPSFLSPVEGPRGAEVIDRGQ*GCRATV *TSVEVWCRGGVNR*EAR*GCSRGRGQECPRRGR YRGRDSAGSEREGRLRGMALWSGQGHDHRKPGK WKQTGACWSSWSFVWGTRGRRQALHPDLPLPLD EGLLCLLDDVLPLLLEEEASQPPPPASRLALLTQ TDLLSLQHPGRPQGGGPGGMGF*GLGSALSPAL ANPLCPSVTVCVHMRACVYLCGVAHKVTPGGGI IRGKV*PPLFRTFIFSSL*IFTLLI*SSAVP*LR IALRCR*PPHQRNPTVSHRLQVCISNCLLNTTR PPDTMNLMCLNPNSTFPVLLPAPPPVFSTLLYD NSIFPNTGFKNPGVVLDSSLSHLSTNSAANTDSS TFAHQSISPNHPIPDCTLSSLPSITAVAS*LVF LIPLLPISSEITPEQLK*SFRMGTVAHACNTST LGGGGWLIPLGWEFKTILTNREKPHLY*KYKN*P DVVGRVCNPNYLGG*DRRTA*TREVEVAVSQNR ATALQPGQEARNSVSK*NNLLKMTGPGGVALFY NPSTLGG*GGQIA*HQGFETSLANMVKLRLY*KY *KLAGRGGGCL*SQLLGRLMQENRLNQADAGCS EPRLRHCTPT*AILCLKNKKKPGKQSETPSLQK SQKISWVWWQ*WHTPVVPATWEAF*YFLQRPGLT LLPGLVLNSWAPTIPFPGPPKVLGLQA*ATVPS LKAYFKG*K*TAI*RDTDRAVWKGPEQELLSP* SWGYVTLWAHSVSLHTFSPQKLPNPVLWAFYGEL HWLSMTEAWTTVIM*LGKKGLI*AQQGQSRFFG PLCSIPFSRVWGKDPLWNEDPTTHNQIRVLPWA AEKRTGEGQRDERLFFEA*GTPT*QRKTVTMVM* VMS*EPWTKPAHIQSSPTSQRLYFHSLCSKLRS L*SLLWHRGIPRGICPGGHSVPTWEPTPACKAS PPSDQQPGQFVVPSSGLMSVLAQNRVGRAGQGPA GRLILEIAWETQQGVGARRRGGAKQVISSPEPQ PLPTVSPWPAPWCSSPAVPRASACTWPYVWLQI HPRASKCMPR*GT*KHRAGCGRRPGPWHALRDPW RRCSWT*GTQNPWPLPGNA*LRAAWTCWCVTQA WACWGRWRRWGRTPWPLCWT*M**GLCGCCRPS CQT*RGAVRDACW*PGAWED*WVSGREWPRQLQI LCVRS*ALKAGSAGGVEWGAVSLEGHRLPGDDP LAAAPQEPRLPT*GLPFNDVYCASKFALEGLCE SLAVLLLPFGVQ*VNTPVPRTLLTLT*RRRAPCP AGAALGRSPWPSLPGSH*LCARHLGWAHGTHCA TC*PSC*SVGIVMGKLQPREVR*LAQGHTAARD PARFES*HQGSLVASDGFGRAAPAGTRVSNVLVI PSALSASLPSAAVLLAVGGRDVVGAGTGVGAGT GAWLASAPSHSLLSGQQLEPDRVRPSAHRLHGE GVGQPRGGAGPHGHPHLPPLLPIPRPQQASLSRG GAEP*GGGGGERRAGLQEWGRCVLRRAAVATAL LPPPQVFLTALRAPKPTLRYFTTERFLPLLRMR LDDPSGSNYVTAMHREVFGDVPAKAEAGAEAGGG AGPGAEDEAGRGAVGDPELGDPPAAPQ*RLPQP LSPAPFFVPWVCVVPGDGAAVAAVGG*LR*IAL ASFTSAARRDGCRL*CQRFGRRRQEDRSSPGVGD QPEQHSETPISTKIKKI

Frame 2

LGGTWVGVESGCDLHSISHETSGKFLAFSECFP FLSLFFSFLFFFCFETEFHSCCPAWSAIARSQF TTTSASRVQV*LGLQACATTPS*FCIFSRDEVS PCWSDWSWTPDLR*SARPGLSECWDYRHDPPHPA L*VFLICKMGIIIPI*SVLVMVEIMPESASIEV *YPARTVIRMNSNMTTVTFCKCVKKKEG*VNQR ETAPLTLFCPRGLSDPHHLGSCLTRRSSWGYRTG YFATMAHLS*AQWRVPGGQKSLIGASRVVEPLS ELLWRSGAGEV*TDRRLGEDAAEEGGRSAPGGG GTEAGIQLGLRGREG*GGWPFGVGRDMTTESLGS GNRQEPAGALGALCGALGEGGRHSTLTCPYLWM RVFSACWMMCCPCSWRKRPPSHPLLPVALPC*P RQICYPYSIQEGHREGAQVAWVSEAWDLLSVPLS PTPFAPL*QCVYICVHVCTCVVWLTRSPLGVG* *EVKCDPLSSVHSFFQACEYSHC*YNLLQSHDF E*HLDADDPPTKETPLSPTDSRSVSPTAYSIQHV HLIQ*T*CV*TPIPHFQSSYRLLPQSSLLYYMT TPFFPILVLKTLE*SLTPVSLTFQPIQQQILTA LPLLIKAYPQITLFQTAHCHLSLQLQQ*PPN*FS *FHSCPSAVKLPQSS*NDLLGWAQWLTPVIPAL WEVEAG*SP*VGSSRPSSPTGRNPISTKNTKIS RMWWDVSVIPTTWEAETGELLEPGRWRLQ*ARIV PQHSSLGKKRETPSQNKIIF*K*QGQVGWHFFI IRALWEAEVGRSLDIRGLRPAWPTW*NSVSTKN TKN*LGVVAGACNPSYSGG*CRRIA*TRQMQDAV SQD*DTALQPKQYFVSKIKKSLGNKVRPRLYKK VKKLAGYGGSDGTHL*SRLLGKLFNIFCRDRVS LCYLAWS*TPGLQQSPFLGLPKCWDYRHEPPCPA SKHILKDRNKQPYEEIQTGRSGRVQSRSFYLHR VGVTSPSGHILSASTRSALRSSRTLSFGPFMEN SIGCP*LKHGQL**CDWAKRV*SKPSKASPDSLG LCAAFLSPGYGARTHSGMRILQPTIRLESCLGQ LKRGQEKVRETKGCFLRPEAPQHDNVRL*PWSC EL*ARNPGRNQHIYNHLPPPNAFTFTASAANCGH YNRSCGTEAYPGESAQGATLCPRGNPHLLVKPP LPLTSNQDSLLFQAVGSCLFWLRTGWGERARDP QEGLSLRLRGRHNKGWGPAGGAGRSR*YQAQSPS LSPQSHHGPHRGAHHRLFLGHRPALGRTSGFRS IPELQSVCHVEGPENTGPAVGGGPGPGMPSGIP GDVAAGRKGLKIRGRCPGTRD*GPRGRAGV*RRP GPAGAAGGAGGGRRGLCAGRECSRDCADAAGLP ARHEEARFGTRVGDRERGRIDG*VVGSGLGSSR FFVCGAEP*RQVPRGGWSGVPSAWRGTVCPGMTP WPLRLRNLVSPPKGCLSMTFIAPASSRSKAYAR VWRFCCCPLGSSESTPPFPEPS*L*PRDAEHPV LREPLWGDLPGPLCPAHISCVPGTWAGRMARIVP PADLAAEVLVLLWGSSSPERLGDWPKVTQRPGT PLDSNPDTRAPL*PQMDLGGLLRQEPAFQMFWL SPAPFPPHFPAQRCCSRSGAGTWLGLGLGLGLGL GPGWRPPPPTRCSPASSLSLIECGPVHTAFMEK VLGSPEEVLDRTDIHTFHRFYQYLAHSKQVFRE AAQNPEEVAEVSAGLDSRSGGGASSGAQRWPQLS SRRRRSSSPLCAPRSRPCATSPPSASCPCCGCA WTTPAAPTTSPPCTGKCSATFRQRPRLGPRLGA GPGLGQRTRPGAVRWGTLSSAILRPPRSKGFLSR CLPRPSLSPGSVWSLGMGRR*QLWVAN*DRSR* PVLPAQLGAMAVACNASALGGGGRRIAQAPELE TSQSNTVRPPSLQK*RKF

Sequence HSD17B1.cAug05

AGGACCCACTCTGGAATGAGGATCCTACAACCCACAATCAGATTAGAGTCCTGCCTTGGGCAGCTGAAAAGAGGACAGGAGAAGGTCAGAGAGACGAAAG GCTGTTTTTTGAGGCCTGAGGCACCCCAACATGACAACGTAAGACTGTAACCATGGTCATGTGAGTTATGAGCTAGGAACCCTGGACGAAACCAGCACAT ATACAATCATCTCCCACCTCCCAACGCCTTTACTTTCACAGCCTCTGCAGCAAACTGCGGTCACTATAATCGCTCCTGTGGCACAGAGGCATACCCAGGG GAATCTGCCCAGGGGGCCACTCTGTGCCCACGTGGGAACCCACACCTGCTTGTAAAGCCTCCCCTCCCTCTGACCAGCAACCAGGACAGTTTGTTGTTCC AAGCAGTGGGCTCATGTCTGTTTTGGCTCAGAACAGGGTGGGGAGAGCGGGCCAGGGACCCGCAGGAAGGCTTATCCTTGAGATTGCGTGGGAGACACAA CAAGGGGTGGGGGCCCGCAGGCGGGGCGGGGCGAAGCAGGTGATATCAAGCCCAGAGCCCCAGCCTCTCCCCACAGTCTCACCATGGCCCGCACCGTGGT GCTCATCACCGGCTGTTCCTCGGGCATCGGCCTGCACTTGGCCGTACGTCTGGCTTCAGATCCATCCCAGAGCTTCAAAGGTATAGATAGGCAGGGACAG GGAGGGAGAGAAGGGAGGAGCCCTTGGAGGCCAGAAGGGAAGTCAGATCTTCCTCCTCTCCCAAAACCTCCAGTGTATGCCACGTTGAGGGACCTGAAAA CACAGGGCCGGCTGTGGGAGGCGGCCCGGGCCCTGGCATGCCCTCCGGGATCCCTGGAGACGTTGCAGCTGGACGTAAGGGACTCAAAATCCGTGGCCGC TGCCCGGGAACGCGTGACTGAGGGCCGCGTGGACGTGCTGGGTGAGCCTCCTGGAAGCATATGGGCTCCTAGGAGCCTTCTCCGCCCTGCGTTGAAACCA ACATGTTCCCAGGCCCAGGGAGCACGAGGGGACAGGCCGTGCTGAGGGTGATGCTGAGGCGGGCTGGTCGGGCCTCTTGTCTCCGCAGTGTGTAACGCAG GCCTGGGCCTGCTGGGGCCGCTGGAGGCGCTGGGGGAGGACGCCGTGGCCTCTGTGCTGGACGTGAATGTAGTAGGGACTGTGCGGATGCTGCAGGCCTT CCTGCCAGACATGAAGAGGCGCGGTTCGGGACGCGTGTTGGTGACCGGGAGCGTGGGAGGATTGATGGGTGAGTGGTAGGGAGTGGCCTCGGCAGCTCCA GATTCTTTGTGTGCGGAGCTGAGCCTTGAAGGCAGGTTCCGCGGGGGGGGTGGAGTGGGGTGCCGTCAGCTTGGAGGGGCACCGTCTGCCCGGGGATGAC CCCCTGGCCGCTGCGCCTCAGGAACCTCGTCTCCCCACCTAAGGGCTGCCTTTCAATGACGTTTATTGCGCCAGCAAGTTCGCGCTCGAAGGCTTATGCG AGAGTCTGGCGGTTCTGCTGCTGCCCTTTGGGGTCCAGTGAGTCAACACCCCCGTTCCCCGAACCCTCTTAACTCTGACCTAGAGACGCCGAGCACCCTG TCCTGCGGGAGCCGCTCTGGGGCGATCTCCCTGGCCCTCTCTGCCCGGCTCACATTAGCTGTGTGCCAGGCACTTGGGCTGGGCGCATGGCACGCATTGT GCCACCTGCTGACCTAGCTGCTGAAGTGTTGGTATTGTTATGGGGAAGCTCCAGCCCAGAGAGGTTAGGTGACTGGCCCAAGGTCACACAGCGGCCAGGG ACCCCGCTAGATTCGAATCCTGACACCAGGGCTCCCTTGTAGCCTCAGATGGATTTGGGAGGGCTGCTCCGGCAGGAACCCGCGTTTCAAATGTTCTGGT TATCCCCAGCGCCCTTTCCGCCTCACTTCCCAGCGCAGCGGTGCTGCTCGCGGTCGGGGGCCGGGACGTGGTTGGGGCTGGGACTGGGGTTGGGGCTGGG ACTGGGGCCTGGCTGGCGTCCGCCCCCTCCCACTCGTTGCTCTCCGGCCAGCAGCTTGAGCCTGATCGAGTGCGGCCCAGTGCACACCGCCTTCATGGAG AAGGTGTTGGGCAGCCCAGAGGAGGTGCTGGACCGCACGGACATCCACACCTTCCACCGCTTCTACCAATACCTCGCCCACAGCAAGCAAGTCTTTCGCG AGGCGGCGCAGAACCCTGAGGAGGTGGCGGAGGTCTTCCTCACCGCTTTGCGCGCCCCGAAGCCGACCCTGCGCTACTTCACCACCGAGCGCTTCCTGCC CCTGCTGCGGATGCGCCTGGACGACCCCAGCGGCTCCAACTACGTCACCGCCATGCACCGGGAAGTGTTCGGCGACGTTCCGGCAAAGGCCGAGGCTGGG GCCGAGGCTGGGGGCGGGGCCGGGCCTGGGGCAGAGGACGAGGCCGGGCGCGGTGCGGTGGGGGACCCTGAGCTCGGCGATCCTCCGGCCGCCCCGCAGT AAAGGCTTCCTCAGCCGCTGTCTCCCGCGCCCTTCTTTGTCCCCTGGGTCTGTGTGGTCCCTGGGGATGGGGCGGCGGTAGCAGCTGTGGGTGGCTAATT AAGATAGATCGCGTTAGCCAGTTTTACCAGCGCAGCTAGGCGCGATGGCTGTCGCCTGTAATGCCAGCGCTTTGGGAGGCGGAGGCAGGAGGATCGCTCA AGCCCCGGAGTTGGAGACCAGCCAGAGCAACACAGTGAGACCCCCATCTCTACAAAAATAAAGAAAATTTAAAAATCAG

HSD17B1.F4 (forward primer)
HSD17B1.R4 (reverse primer)
Intron retention

Frame 0

RTHSGMRILQPTIRLESCLGQLKRGQEKVRETK GCFLRPEAPQHDNVRL*PWSCEL*ARNPGRNQHI YNHLPPPNAFTFTASAANCGHYNRSCGTEAYPG ESAQGATLCPRGNPHLLVKPPLPLTSNQDSLLF QAVGSCLFWLRTGWGERARDPQEGLSLRLRGRHN KGWGPAGGAGRSR*YQAQSPSLSPQSHHGPHRG AHHRLFLGHRPALGRTSGFRSIPELQRYR*AGT GRERREEPLEARREVRSSSSPKTSSVCHVEGPEN TGPAVGGGPGPGMPSGIPGDVAAGRKGLKIRGR CPGTRD*GPRGRAG*ASWKHMGS*EPSPPCVET NMFPGPGSTRGQAVLRVMLRRAGRASCLRSV*RR PGPAGAAGGAGGGRRGLCAGRECSRDCADAAGL PARHEEARFGTRVGDRERGRIDG*VVGSGLGSS RFFVCGAEP*RQVPRGGWSGVPSAWRGTVCPGMT PWPLRLRNLVSPPKGCLSMTFIAPASSRSKAYA RVWRFCCCPLGSSESTPPFPEPS*L*PRDAEHP VLREPLWGDLPGPLCPAHISCVPGTWAGRMARIV PPADLAAEVLVLLWGSSSPERLGDWPKVTQRPG TPLDSNPDTRAPL*PQMDLGGLLRQEPAFQMFW LSPAPFPPHFPAQRCCSRSGAGTWLGLGLGLGLG LGPGWRPPPPTRCSPASSLSLIECGPVHTAFME KVLGSPEEVLDRTDIHTFHRFYQYLAHSKQVFR EAAQNPEEVAEVFLTALRAPKPTLRYFTTERFLP LLRMRLDDPSGSNYVTAMHREVFGDVPAKAEAG AEAGGGAGPGAEDEAGRGAVGDPELGDPPAAPQ *RLPQPLSPAPFFVPWVCVVPGDGAAVAAVGG*L R*IALASFTSAARRDGCRL*CQRFGRRRQEDRS SPGVGDQPEQHSETPISTKIKKI*KS

Frame 1

GPTLE*GSYNPQSD*SPALGS*KEDRRRSERRK AVF*GLRHPNMTT*DCNHGHVSYELGTLDETST YTIISHLPTPLLSQPLQQTAVTIIAPVAQRHTQG NLPRGPLCAHVGTHTCL*SLPSL*PATRTVCCS KQWAHVCFGSEQGGESGPGTRRKAYP*DCVGDT TRGGGPQAGRGEAGDIKPRAPASPHSLTMARTVV LITGCSSGIGLHLAVRLASDPSQSFKGIDRQGQ GGREGRSPWRPEGKSDLPPLPKPPVYATLRDLK TQGRLWEAARALACPPGSLETLQLDVRDSKSVAA ARERVTEGRVDVLGEPPGSIWAPRSLLRPALKP TCSQAQGARGDRPC*G*C*GGLVGPLVSAVCNA GLGLLGPLEALGEDAVASVLDVNVVGTVRMLQAF LPDMKRRGSGRVLVTGSVGGLMGEW*GVASAAP DSLCAELSLEGRFRGGGGVGCRQLGGAPSARG* PPGRCASGTSSPHLRAAFQ*RLLRQQVRARRLMR ESGGSAAALWGPVSQHPRSPNPLNSDLETPSTL SCGSRSGAISLALSARLTLAVCQALGLGAWHAL CHLLT*LLKCWYCYGEAPAQRG*VTGPRSHSGQG PR*IRILTPGLPCSLRWIWEGCSGRNPRFKCSG YPQRPFRLTSQRSGAARGRGPGRGWGWDWGWGW DWGLAGVRPLPLVALRPAA*A*SSAAQCTPPSWR RCWAAQRRCWTARTSTPSTASTNTSPTASKSFA RRRRTLRRWRRSSSPLCAPRSRPCATSPPSASC PCCGCAWTTPAAPTTSPPCTGKCSATFRQRPRLG PRLGAGPGLGQRTRPGAVRWGTLSSAILRPPRS KGFLSRCLPRPSLSPGSVWSLGMGRR*QLWVAN *DRSR*PVLPAQLGAMAVACNASALGGGGRRIAQ APELETSQSNTVRPPSLQK*RKFKNQ

Frame 2

DPLWNEDPTTHNQIRVLPWAAEKRTGEGQRDER LFFEA*GTPT*QRKTVTMVM*VMS*EPWTKPAH IQSSPTSQRLYFHSLCSKLRSL*SLLWHRGIPR GICPGGHSVPTWEPTPACKASPPSDQQPGQFVVP SSGLMSVLAQNRVGRAGQGPAGRLILEIAWETQ QGVGARRRGGAKQVISSPEPQPLPTVSPWPAPW CSSPAVPRASACTWPYVWLQIHPRASKV*IGRDR EGEKGGALGGQKGSQIFLLSQNLQCMPR*GT*K HRAGCGRRPGPWHALRDPWRRCSWT*GTQNPWP LPGNA*LRAAWTCWVSLLEAYGLLGAFSALR*NQ HVPRPREHEGTGRAEGDAEAGWSGLLSPQCVTQ AWACWGRWRRWGRTPWPLCWT*M**GLCGCCRP SCQT*RGAVRDACW*PGAWED*WVSGREWPRQLQ ILCVRS*ALKAGSAGGVEWGAVSLEGHRLPGDD PLAAAPQEPRLPT*GLPFNDVYCASKFALEGLC ESLAVLLLPFGVQ*VNTPVPRTLLTLT*RRRAPC PAGAALGRSPWPSLPGSH*LCARHLGWAHGTHC ATC*PSC*SVGIVMGKLQPREVR*LAQGHTAAR DPARFES*HQGSLVASDGFGRAAPAGTRVSNVLV IPSALSASLPSAAVLLAVGGRDVVGAGTGVGAG TGAWLASAPSHSLLSGQQLEPDRVRPSAHRLHG EGVGQPRGGAGPHGHPHLPPLLPIPRPQQASLSR GGAEP*GGGGGLPHRFARPEADPALLHHRALPA PAADAPGRPQRLQLRHRHAPGSVRRRSGKGRGW GRGWGRGRAWGRGRGRARCGGGP*ARRSSGRPAV KASSAAVSRALLCPLGLCGPWGWGGGSSCGWLI KIDRVSQFYQRS*ARWLSPVMPALWEAEAGGSL KPRSWRPARATQ*DPHLYKNKENLKI