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Sequences

Sequence SHC1.kAug05

SHC1.F7 (forward primer)
SHC1.R7 (reverse primer)
Single exon skipping

Frame 0

SVKEGP*GRGSGVSEAGSECNKVSPGRQGPGRK LER*PKLAVA*SGTKSARGAVRRLHEALDMNKLS GGGGRRTRVEGGQLGGEEWTRHGSFVNKPTRGW LHPNDKVMGPGVSYLVRYMGCVEVLQSMRALDF NTRTQVTREAISLVCEAVPGAKGATRRRKPCSRP LSSILGRSNLKFAGMPITLTVSTSSLNLMAADC KQDTAEYVAYVAKDPVNQRACHILECPEGLAQD V

Frame 1

A*RRDPEVEGQGLVRPEVSVIKFLQGGRARGES WSGNLSWQWRDPAPNRPAVRCGDSMRPWT*TS* VEAAGAGLGWKGASLGARSGPATGALSISPRGAG CIPTTKSWDPGFPTWFGTWVVWRSSSQCVPWTS TPGLRSPGRPSVWCVRLCRVLRGRQGGESPVAA RSALSWGGVT*NLLECQSLSPSPPAASTSWPQTA NRTQPSMSPMLPKTL*IREPATFWSVPKGLPRM

Frame 2

REGGTLR*RVRG**GRK*V**SFSREAGPGEKV GAVT*AGSGVIRHQIGPRCGAETP*GPGHEQAE WRRRAQDSGGRGPAWGRGVDPPRELCQ*AHAGL AASQRQSHGTRGFLLGSVHGLCGGPPVNACPGLQ HPDSGHQGGHQSGV*GCAGC*GGDKEEKAL*PP AQLYPGEE*PEICWNANHSHRLHQQPQPHGRRL QTGHSRVCRLCCQRPCESESLPHSGVSRRACPGC

Sequence SHC1.jAug05

SHC1.F7 (forward primer)
SHC1.R7 (reverse primer)
Single exon skipping

Frame 0

PS*VGGRQAGAGSEYPSFPREAISLVCEAVPGA KGATRRRKPCSRPLSSILGRSNLKFAGMPITLTV STSSLNLMAADCKQDTAEYVAYVAKDPVNQRAC HILECPEGLAQDVISTIGQAFELRFKQYLRNPP KLVTPHDRMAGFDGSAWDEEEEEPPDHQYYNDFP GKEPPLGGVVDMRLREGAAPGAARPTAPNAQTP SHLGATL

Frame 1

PVEWEAGRQALGLSTPLSPGRPSVWCVRLCRVL RGRQGGESPVAARSALSWGGVT*NLLECQSLSP SPPAASTSWPQTANRTQPSMSPMLPKTL*IREPA TFWSVPKGLPRMSSAPLARPSSCASNNTSGTHP NWSPLMTGWLALMAQHGMRRRKSHLTISTIMTS RGRNPPWGGW*T*GFGKEPLQGLLDPLHPMPRPP ATWELH

Frame 2

QLSGRQAGRRWV*VPLFPQGGHQSGV*GCAGC* GGDKEEKAL*PPAQLYPGEE*PEICWNANHSHR LHQQPQPHGRRLQTGHSRVCRLCCQRPCESESL PHSGVSRRACPGCHQHHWPGLRVALQTIPQEPTQ TGHPS*QDGWL*WLSMG*GGGRAT*PSVL**LP GEGTPLGGGGRHEASGRSRSRGCSTHCTQCPDP QPLGSYI

Sequence SHC1.cAug05

TCTTCTCAGCCAGAGAAGAGGCTCCACGTTGGGCGGGGATGGGGCCTGAAACTGTCTGGGTCTGAGCTGGGGAGCGGAAGCCACTTGTCCCTCTCCCTCC CCAGGACTTCTGTGACTCCTGGGCCACAGAGGTCCAACCAGGCTGGGCAGCAGCCCCTCTTTCACCTCAACTATGGATCTCCTGCCCCCCAAGCCCAAGT ACAATCCACTCCGGAATGAGTCTCTGTCATCGCTGGAGGAAGGGGCTTCTGGGTCCACCCCCCCGGAGGAGCTGCCTTCCCCATCAGCTTCATCCCTGGG GCCCATCCTGCCTCCTCTGCCTGGGGACGATAGTCCCACTACCCTGTGCTCCTTCTTCCCCCGGATGAGCAACCTGAGGCTGGCCAACCCGGCTGGGGGG CGCCCAGGGTCTAAGGGGGAGCCAGGAAGGGCAGCTGATGATGGGGAGGGGATCGTAGGGGCAGCCATGCCAGACTCAGGCCCCCTACCCCTCCTCCAGG ACATGAACAAGCTGAGTGGAGGCGGCGGGCGCAGGACTCGGGTGGAAGGGGGCCAGCTTGGGGGCGAGGAGTGGACCCGCCACGGGAGCTTTGTCAATAA GCCCACGCGGGGCTGGCTGCATCCCAACGACAAAGTCATGGGACCCGGGGTTTCCTACTTGGTTCGGTACATGGGTTGTGTGGAGGTCCTCCAGTCAATG CGTGCCCTGGACTTCAACACCCGGACTCAGGTCACCAGGGAGGCCATCAGTCTGGTGTGTGAGGCTGTGCCGGGTGCTAAGGGGGCGACAAGGAGGAGAA AGCCCTGTAGCCGCCCGCTCAGCTCTATCCTGGGGAGGAGTAACCTGAAATTTGCTGGAATGCCAATCACTCTCACCGTCTCCACCAGCAGCCTCAACCT CATGGCCGCAGACTGCAAACAGGACACAGCCGAGTATGTCGCCTATGTTGCCAAAGACCCTGTGAATCAGAGAGCCTGCCACATTCTGGAGTGTCCCGAA GGGCTTGCCCAGGATGTCATCAGCACCATTGGCCAGGCCTTCGAGTTGCGCTTCAAACAATACCTCAGGAACCCACCCAAACTGGTCACCCCTCATGACA GGATGGCTGGCTTTGATGGCTCAGCATGGGATGAGGAGGAGGAAGAGCCACCTGACCATCAGTACTATAATGACTTCCCGGGGAAGGAACCCCCCTTGGG GGGGGTGGTAGACATGAGGCTTCGGGAAGGAGCCGCTCCAGGGGCTGCTCGACCCACTGCACCCAATGCCCAGACCCCCAGCCACTTGGGAGCTACATTG CCTGTAGGACAGCCTGTTGGGGGAGATCCAGAAGTCCGCAAACAGATGCCACCTCCACCACCCTGTCCAGCAGGCAGAGAGCTTTTTGATGATCCCTCCT ATGTCAACGTCCAGAACCTAGACAAGGCCCGGCAAGCAGTGGGTGGTGCTGGGCCCCCCAATCCTGCTATCAATGGCAGTGCACCCCGGGACCTGTTTGA CATGAAGCCCTTCGAAGATGCTCTTCGCGTGCCTCCACCTCCCCAGTCGGTGTCCATGGCTGAGCAGCTCCGAGGGGAGCCCTGGTTCCATGGGAAGCTG AGCCGGCGGGAGGCTGAGGCACTGCTGCAGCTCAATGGGGACTTCCTGGTACGGGAGAGCACGACCACACCTGGCCAGTATGTGCTCACTGGCTTGCAGA GTGGGCAGCCTAAGCATTTGCTACTGGTGGACCCTGAGGGTGTGAGATGGGGTTTCGCCATGTTGCCCAAGCTGTTCTTGAACTCCCGGGCTCAAGTGAT CCGCCTCCCTCGGCCTCCCAGAGTGCTGGGATTACAGGCACGGACCACCATGCCCAGCCTCCACATCTTTTTTTGCACTGTGTATACTCTTCTGAGACAT GCCAACTTCCTCCAGGTCAAGAAAGGGGTATATAGCTCTCAGCTTCACTCTTTCAGGGCTGATGTCGCCTTTGCCTTTTCTCACTTCACTGACCTGTCTA TTCCTACAACTGTCTCTTTCTAGAGAAGCCTCAATGATCAGGATTGACAGGCCACACTCTCCCCCACCATTTTTTTCTCCTCCTTCAAGCCTCTTGTCTG TTTCACCCTCTTCCACCTTGGAGGCTGAGGTCTTATTTGACTCTTCACCTGAATTGACCTTCTTCCTTCCCACCCTCCCAGGTTCGGACTAAGGATCACC GCTTTGAAAGTGTCAGTCACCTTATCAGCTACCACATGGACAATCACTTGCCCATCATCTCTGCGGGCAGCGAACTGTGTCTACAGCAACCTGTGGAGCG GAAACTGTGATCTGCCCTAGCGCTCTCTTCCAGAAGATGCCCTCCAATCCTTTCCACCCTATTCCCTAACTCTCGGGACCTCGTTTGGGAGTGTTCTGTG GGCTTGGCCTTGTGTCAGAGCTGGGAGTAGCATGGACTCTGGGTTTCATATCCAGCTGAGTGAGAGGGTTTGAGTCAAAAGCCTGGGTGAGAATCCTGCC TCTCCCCAAACATTAATCACCAAAGTATTAATGTACAGAGTGGCCCCTCACCTGGGCCTTTCCTGTGCCAACCTGATGCCCCTTCCCCAAGAAGGTGAGT GCTTGTCATGGAAAATGTCCTGTGGTGACAGGCCCAGTGGAACAGTCACCCTTCTGGGCAAGGGGGAACAAATCACACCTCTGGGCTTCAGGGTATCCCA GACCCCTCTCAACACCCGCCCCCCCCATGTTTAAACTTTGTGCCTTTGACCATCTCTTAGGTCTAATGATATTTTATGCAAACAGTTCTTGGACCCCTGA ATTCAATGACAGGGATGCCAACACCTTCTTGGCTTCTGGGACCTGTGTTCTTGCTGAGCACCCTCTCCGGTTTGGGTTGGGATAACAGAGGCAGGAGTGG CAGCTGTCCCCTCTCCCTGGGGATATGCAACCCTTAGAGATTGCCCCAGAGCCCCACTCCCGGCCAGGCGGGAGATGGACCCCTCCCTTGCTCAGTGCCT CCTGGCCGGGGCCCCTCACCCCAAGGGGTCTGTATATACATTTCATAAGGCCTGCCCTCCCATGTTGCATGCCTATGTACTCTACGCCAAAGTGCAGCCC TTCCTCCTGAAGCCTCTGCCCTGCCTCCCTTTCTGGGAGGGCGGGGTGGGGGTGACTGAATTTGGGCCTCTTGTACAGTTAACTCTCCCAGGTGGATTTT GTGGAGGTGAGAAAAGGGGCATTGAGACTATAAAGCAGTAGACAATCCCCACATACCATCTGTAGAGTTGGAACTGCATTCTTTTAAAGTTTTATATGCA TATATTTTAGGGCTGTAGACTTACTTTCCTATTTTCTTTTCCATTGCTTATTCTTGAGCACAAAATGATAATCAATTATTACATTTATACATCACCTTTT TGACTTTTCCAAGCCCTTTTACAGCTCTTGGCATTTTCCTCGCCTAGGCCTGTGAGGTAACTGGGATCGCACCTTTTATACCAGAGACCTGAGGCAGATG AAATTTATTTCCATCTAGGACTAGAAAAACTTGGGTCTCTTACCGCGAGACTGAGAGGCAGAAGTCAGCCCGAATGCCTGTCAGTTTCATGGAGGGGAAA CGCAAAACCTGCAGTTCCTGAGTACCTTCTACAGGCCCGGCCCAGCCTAGGCCCGGGGTGGCCACACCACAGCAAGCCGGCCCCCCCTCTTTTGGCCTTG TGGATAAGGGAGAGTTGACCGTTTTCATCCTGGCCTCCTTTTGCTGTTTGGATGTTTCCACGGGTCTCACTTATACCAAAGGGAAAACTCTTCATTAAAG TCCGTATTTCTTCT

SHC1.F7 (forward primer)
SHC1.R7 (reverse primer)
Single exon skipping

Frame 0

SSQPEKRLHVGRGWGLKLSGSELGSGSHLSLSL PRTSVTPGPQRSNQAGQQPLFHLNYGSPAPQAQV QSTPE*VSVIAGGRGFWVHPPGGAAFPISFIPG AHPASSAWGR*SHYPVLLLPPDEQPEAGQPGWG APRV*GGARKGS**WGGDRRGSHARLRPPTPPPG HEQAEWRRRAQDSGGRGPAWGRGVDPPRELCQ* AHAGLAASQRQSHGTRGFLLGSVHGLCGGPPVN ACPGLQHPDSGHQGGHQSGV*GCAGC*GGDKEEK AL*PPAQLYPGEE*PEICWNANHSHRLHQQPQP HGRRLQTGHSRVCRLCCQRPCESESLPHSGVSR RACPGCHQHHWPGLRVALQTIPQEPTQTGHPS*Q DGWL*WLSMG*GGGRAT*PSVL**LPGEGTPLG GGGRHEASGRSRSRGCSTHCTQCPDPQPLGSYI ACRTACWGRSRSPQTDATSTTLSSRQRAF**SLL CQRPEPRQGPASSGWCWAPQSCYQWQCTPGPV* HEALRRCSSRASTSPVGVHG*AAPRGALVPWEA EPAGG*GTAAAQWGLPGTGEHDHTWPVCAHWLAE WAA*AFATGGP*GCEMGFRHVAQAVLELPGSSD PPPSASQSAGITGTDHHAQPPHLFLHCVYSSET CQLPPGQERGI*LSASLFQG*CRLCLFSLH*PVY SYNCLFLEKPQ*SGLTGHTLPHHFFLLLQASCL FHPLPPWRLRSYLTLHLN*PSSFPPSQVRTKDH RFESVSHLISYHMDNHLPIISAGSELCLQQPVER KL*SALALSSRRCPPILSTLFPNSRDLVWECSV GLALCQSWE*HGLWVSYPAE*EGLSQKPG*ESC LSPNINHQSINVQSGPSPGPFLCQPDAPSPRR*V LVMENVLW*QAQWNSHPSGQGGTNHTSGLQGIP DPSQHPPPPCLNFVPLTIS*V**YFMQTVLGPL NSMTGMPTPSWLLGPVFLLSTLSGLGWDNRGRSG SCPLSLGICNP*RLPQSPTPGQAGDGPLPCSVP PGRGPSPQGVCIYIS*GLPSHVACLCTLRQSAA LPPEASALPPFLGGRGGGD*IWASCTVNSPRWIL WR*EKGH*DYKAVDNPHIPSVELELHSFKVLYA YILGL*TYFPIFFSIAYS*AQNDNQLLHLYITF LTFPSPFTALGIFLA*ACEVTGIAPFIPET*GR* NLFPSRTRKTWVSYRETERQKSARMPVSFMEGK RKTCSS*VPSTGPAQPRPGVATPQQAGPPSFGL VDKGELTVFILASFCCLDVSTGLTYTKGKTLH*S PYFF

Frame 1

LLSQRRGSTLGGDGA*NCLGLSWGAEATCPSPS PGLL*LLGHRGPTRLGSSPSFTSTMDLLPPKPK YNPLRNESLSSLEEGASGSTPPEELPSPSASSLG PILPPLPGDDSPTTLCSFFPRMSNLRLANPAGG RPGSKGEPGRAADDGEGIVGAAMPDSGPLPLLQ DMNKLSGGGGRRTRVEGGQLGGEEWTRHGSFVNK PTRGWLHPNDKVMGPGVSYLVRYMGCVEVLQSM RALDFNTRTQVTREAISLVCEAVPGAKGATRRR KPCSRPLSSILGRSNLKFAGMPITLTVSTSSLNL MAADCKQDTAEYVAYVAKDPVNQRACHILECPE GLAQDVISTIGQAFELRFKQYLRNPPKLVTPHD RMAGFDGSAWDEEEEEPPDHQYYNDFPGKEPPLG GVVDMRLREGAAPGAARPTAPNAQTPSHLGATL PVGQPVGGDPEVRKQMPPPPPCPAGRELFDDPS YVNVQNLDKARQAVGGAGPPNPAINGSAPRDLFD MKPFEDALRVPPPPQSVSMAEQLRGEPWFHGKL SRREAEALLQLNGDFLVRESTTTPGQYVLTGLQ SGQPKHLLLVDPEGVRWGFAMLPKLFLNSRAQVI RLPRPPRVLGLQARTTMPSLHIFFCTVYTLLRH ANFLQVKKGVYSSQLHSFRADVAFAFSHFTDLS IPTTVSF*RSLNDQD*QATLSPTIFFSSFKPLVC FTLFHLGG*GLI*LFT*IDLLPSHPPRFGLRIT ALKVSVTLSATTWTITCPSSLRAANCVYSNLWS GNCDLP*RSLPEDALQSFPPYSLTLGTSFGSVLW AWPCVRAGSSMDSGFHIQLSERV*VKSLGENPA SPQTLITKVLMYRVAPHLGLSCANLMPLPQEGE CLSWKMSCGDRPSGTVTLLGKGEQITPLGFRVSQ TPLNTRPPHV*TLCL*PSLRSNDILCKQFLDP* IQ*QGCQHLLGFWDLCSC*APSPVWVGITEAGV AAVPSPWGYATLRDCPRAPLPARREMDPSLAQCL LAGAPHPKGSVYTFHKACPPMLHAYVLYAKVQP FLLKPLPCLPFWEGGVGVTEFGPLVQLTLPGGF CGGEKRGIETIKQ*TIPTYHL*SWNCILLKFYMH IF*GCRLTFLFSFPLLILEHKMIINYYIYTSPF *LFQALLQLLAFSSPRPVR*LGSHLLYQRPEAD EIYFHLGLEKLGSLTARLRGRSQPECLSVSWRGN AKPAVPEYLLQARPSLGPGWPHHSKPAPPLLAL WIRES*PFSSWPPFAVWMFPRVSLIPKGKLFIK VRISS

Frame 2

FSAREEAPRWAGMGPETVWV*AGERKPLVPLPP QDFCDSWATEVQPGWAAAPLSPQLWISCPPSPS TIHSGMSLCHRWRKGLLGPPPRRSCLPHQLHPW GPSCLLCLGTIVPLPCAPSSPG*AT*GWPTRLGG AQGLRGSQEGQLMMGRGS*GQPCQTQAPYPSSR T*TS*VEAAGAGLGWKGASLGARSGPATGALSI SPRGAGCIPTTKSWDPGFPTWFGTWVVWRSSSQC VPWTSTPGLRSPGRPSVWCVRLCRVLRGRQGGE SPVAARSALSWGGVT*NLLECQSLSPSPPAAST SWPQTANRTQPSMSPMLPKTL*IREPATFWSVPK GLPRMSSAPLARPSSCASNNTSGTHPNWSPLMT GWLALMAQHGMRRRKSHLTISTIMTSRGRNPPW GGW*T*GFGKEPLQGLLDPLHPMPRPPATWELHC L*DSLLGEIQKSANRCHLHHPVQQAESFLMIPP MSTSRT*TRPGKQWVVLGPPILLSMAVHPGTCL T*SPSKMLFACLHLPSRCPWLSSSEGSPGSMGS* AGGRLRHCCSSMGTSWYGRARPHLASMCSLACR VGSLSICYWWTLRV*DGVSPCCPSCS*TPGLK* SASLGLPECWDYRHGPPCPASTSFFALCILF*DM PTSSRSRKGYIALSFTLSGLMSPLPFLTSLTCL FLQLSLSREASMIRIDRPHSPPPFFSPPSSLLS VSPSSTLEAEVLFDSSPELTFFLPTLPGSD*GSP L*KCQSPYQLPHGQSLAHHLCGQRTVSTATCGA ETVICPSALFQKMPSNPFHPIP*LSGPRLGVFC GLGLVSELGVAWTLGFISS*VRGFESKAWVRILP LPKH*SPKY*CTEWPLTWAFPVPT*CPFPKKVS ACHGKCPVVTGPVEQSPFWARGNKSHLWASGYP RPLSTPAPPMFKLCAFDHLLGLMIFYANSSWTPE FNDRDANTFLASGTCVLAEHPLRFGLG*QRQEW QLSPLPGDMQPLEIAPEPHSRPGGRWTPPLLSA SWPGPLTPRGLYIHFIRPALPCCMPMYSTPKCSP SS*SLCPASLSGRAGWG*LNLGLLYS*LSQVDF VEVRKGALRL*SSRQSPHTICRVGTAFF*SFIC IYFRAVDLLSYFLFHCLFLSTK**SIITFIHHLF DFSKPFYSSWHFPRLGL*GNWDRTFYTRDLRQM KFISI*D*KNLGLLPRD*EAEVSPNACQFHGGE TQNLQFLSTFYRPGPA*ARGGHTTASRPPLFWPC G*GRVDRFHPGLLLLFGCFHGSHLYQRENSSLK SVFL

Sequence SHC1.iAug05

SHC1.F7 (forward primer)
SHC1.R7 (reverse primer)
Single exon skipping

Frame 0

SVKEGP*GRGSGVSEAGSECNKVSPGRQGPGRK LER*PKLAVA*SGTKSARGAVRRLHEALTEFRSY RPGWSAMVRSRLTATSAS*VQVILLPQPPE*LG LQAPTTTPG*FLYF**RRGFPMLARLASNS*PQ DMNKLSGGGGRRTRVEGGQLGGEEWTRHGSFVNK PTRGWLHPNDKVMGPGVSYLVRYMGCVEVLQSM RALDFNTRTQVTREAISLVCEAVPGAKGATRRR KPCSRPLSSILGRSNLKFAGMPITLTVSTSSLNL MAADCKQIIANHHMQSISFASGGDPDTAEYVAY VAKDPVNQRACHILECPEGLAQDVISTIGQAFE LRFDGWL*WLSMG*GGGRAT*PSVL**LPGEGTP LGGGGRHEASGRSRSRGCSTHCTQCPDPQPLGS YI

Frame 1

A*RRDPEVEGQGLVRPEVSVIKFLQGGRARGES WSGNLSWQWRDPAPNRPAVRCGDSMRP*RSFAL IVQAGVQWCDLGSLQPPPPEFK*FSCLSLLSSWD YRRPPPRPANFCIFSRDGVSPCWLG*PRTPDLR T*TS*VEAAGAGLGWKGASLGARSGPATGALSI SPRGAGCIPTTKSWDPGFPTWFGTWVVWRSSSQC VPWTSTPGLRSPGRPSVWCVRLCRVLRGRQGGE SPVAARSALSWGGVT*NLLECQSLSPSPPAAST SWPQTANRSSPTTTCNLSHLHPAGIRTQPSMSPM LPKTL*IREPATFWSVPKGLPRMSSAPLARPSS CASMAGFDGSAWDEEEEEPPDHQYYNDFPGKEP PLGGVVDMRLREGAAPGAARPTAPNAQTPSHLGA TL

Frame 2

REGGTLR*RVRG**GRK*V**SFSREAGPGEKV GAVT*AGSGVIRHQIGPRCGAETP*GPDGVSLL SSRLECNGAISAHCNLRLLSSSDSPASAS*VAG ITGAHHHARLIFVFLVETGFPHVG*ASLELLTSG HEQAEWRRRAQDSGGRGPAWGRGVDPPRELCQ* AHAGLAASQRQSHGTRGFLLGSVHGLCGGPPVN ACPGLQHPDSGHQGGHQSGV*GCAGC*GGDKEEK AL*PPAQLYPGEE*PEICWNANHSHRLHQQPQP HGRRLQTDHRQPPHAIYLICIRRGSGHSRVCRL CCQRPCESESLPHSGVSRRACPGCHQHHWPGLRV ALRWLALMAQHGMRRRKSHLTISTIMTSRGRNP PWGGW*T*GFGKEPLQGLLDPLHPMPRPPATWE LH

Sequence SHC1.hAug05

GGGTTTCCCCATGTTGGCTAGGCTAGCCTCGAACTCCTGACCTCAGCTCTATCCTGGGGAGGAGTAACCTGAAATTTGCTGGAATGCCAATCACTCTCAC CGTCTCCACCAGCAGCCTCAACCTCATGGCCGCAGACTGCAAACAGATCATCGCCAACCACCACATGCAATCTATCTCATTTGCATCCGGCGGGGATCCG GACACAGCCGAGTATGTCGCCTATGTTGCCAAAGACCCTGTGAATCAGAGAGCCTGCCACATTCTGGAGTGTCCCGAAGGGCTTGCCCAGGATGTCATCA GCACCATTGGCCAGGCCTTCGAGTTGCGCTTCAAACAATACCTCAGGAACCCACCCAAACTGGTCACCCCTCATGACAGGATGGCTGGCTTTGATGGCTC AGCATGGGATGAGGAGGAGGAAGAGCCACCTGACCATCAGTACTATAATGACTTCCCGGGGAAGGAACCCCCCTTGGGGGGGGTGGTAGACATGAGGCTT CGGGAAGGAGCCGCTCCAGGGGCTGCTCGACCCACTGCACCCAATGCCCAGACCCCCAGCCACTTGGGAGCTACATTGCCTGTAGGACAGCCTGTTGGGG GAGATCCAGAAGTCCGCAAACAGATGCCACCTCCACCACCCTGTCCAGCAGGCAGAGAGCTTTTTGATGATCCCTCCTATGTCAACGTCCAGAACCTAGA CAAGGCCCGGCAAGCAGTGGGTGGTGCTG

SHC1.F7 (forward primer)
SHC1.R7 (reverse primer)
Single exon skipping

Frame 0

GFPHVG*ASLELLTSALSWGGVT*NLLECQSLS PSPPAASTSWPQTANRSSPTTTCNLSHLHPAGIR TQPSMSPMLPKTL*IREPATFWSVPKGLPRMSS APLARPSSCASNNTSGTHPNWSPLMTGWLALMA QHGMRRRKSHLTISTIMTSRGRNPPWGGW*T*GF GKEPLQGLLDPLHPMPRPPATWELHCL*DSLLG EIQKSANRCHLHHPVQQAESFLMIPPMSTSRT* TRPGKQWVVL

Frame 1

GFPMLARLASNS*PQLYPGEE*PEICWNANHSH RLHQQPQPHGRRLQTDHRQPPHAIYLICIRRGS GHSRVCRLCCQRPCESESLPHSGVSRRACPGCHQ HHWPGLRVALQTIPQEPTQTGHPS*QDGWL*WL SMG*GGGRAT*PSVL**LPGEGTPLGGGGRHEA SGRSRSRGCSTHCTQCPDPQPLGSYIACRTACWG RSRSPQTDATSTTLSSRQRAF**SLLCQRPEPR QGPASSGWC

Frame 2

VSPCWLG*PRTPDLSSILGRSNLKFAGMPITLT VSTSSLNLMAADCKQIIANHHMQSISFASGGDP DTAEYVAYVAKDPVNQRACHILECPEGLAQDVI STIGQAFELRFKQYLRNPPKLVTPHDRMAGFDGS AWDEEEEEPPDHQYYNDFPGKEPPLGGVVDMRL REGAAPGAARPTAPNAQTPSHLGATLPVGQPVG GDPEVRKQMPPPPPCPAGRELFDDPSYVNVQNLD KARQAVGGA

Sequence SHC1.gAug05

TCTTCTCAGCCAGAGAAGAGGCTCCACGTTGGGCGGGGATGGGGCCTGAAACTGTCTGGGTCTGAGCTGGGGAGCGGAAGCCACTTGTCCCTCTCCCTCC CCAGGACTTCTGTGACTCCTGGGCCACAGAGGTCCAACCAGGCTAAGGGCCTGGGGATACCCCCTGCCTGGCCCCCTTGCCCAAACTGGCAGGGGGGCCA GGCTGGGCAGCAGCCCCTCTTTCACCTCAACTATGGATCTCCTGCCCCCCAAGCCCAAGTACAATCCACTCCGGAATGAGTCTCTGTCATCGCTGGAGGA GTAACCTGAAATTTGCTGGAATGCCAATCACTCTCACCGTCTCCACCAGCAGCCTCAACCTCATGGCCGCAGACTGCAAACAGATCATCGCCAACCACCA CATGCAATCTATCTCATTTGCATCCGGCGGGGATCCGGACACAGCCGAGTATGTCGCCTATGTTGCCAAAGACCCTGTGAATCAGAGAGCCTGCCACATT CTGGAGTGTCCCGAAGGGCTTGCCCAGGATGTCATCAGCACCATTGGCCAGGCCTTCGAGTTGCGCTTCAAACAATACCTCAGGAACCCACCCAAACTGG TCACCCCTCATGACAGGATGGCTGGCTTTGATGGCTCAGCATGGGATGAGGAGGAGGAAGAGCCACCTGACCATCAGTACTATAATGACTTCCCGGGGAA GGAACCCCCCTTGGGGGGGGTGGTAGACATGAGGCTTCGGGAAGGAGCCGCTCCAGGGGCTGCTCGACCCACTGCACCCAATGCCCAGACCCCCAGCCAC TTGGGAGCTACATTGCCTGTAGGACAGCCTGTTGGGGGAGATCCAGAAGTCCGCAAACAGATGCCACCTCCACCACCCTGTCCAGGCAGAGAGCTTTTTG ATGATCCCTCCTATGTCAACGTCCAGAACCTAGACAAGGCCCGGCAAGCAGTGGGTGGTGCTGGGCCCCCCAATCCTGCTATCAATGGCAGTGCACCCCG GGACCTGTTTGACATGAAGCCCTTCGAAGATGCTCTTCGCGTGCCTCCACCTCCCCAGTCGGTGTCCATGGCTGAGCAGCTCCGAGGGGAGCCCTGGTTC CATGGGAAGCTGAGCCGGCGGGAGGCTGAGGCACTGCTGCAGCTCAATGGGGACTTCCTGGTACGGGAGAGCACGACCACACCTGGCCAGTATGTGCTCA CTGGCTTGCAGAGTGGGCAGCCTAAGCATTTGCTACTGGTGGACCCTGAGGGTGTGGTTCGGACTAAGGATCACCGCTTTGAAAGTGTCAGTCACCTTAT CAGCTACCACATGGACAATCACTTGCCCATCATCTCTGCGGGCAGCGAACTGTGTCTACAGCAACCTGTGGAGCGGAAACTGTGATCTGCCCTAGCGCTC TCTTCCAGAAGATGCCCTCCAATCCTTTCCACCCTATTCCCTAACTCTCGGGACCTCGTTTGGGAGTGTTCTGTGGGCTTGGCCTTGTGTCAGAGCTGGG AGTAGCATGGACTCTGGGTTTCATATCCAGCTGAGTGAGAGGGTTTGAGTCAAAAGCCTGGGTGAGAATCCTGCCTCTCCCCAAACATTAATCACCAAAG TATTAATGTACAGAGTGGCCCCTCACCTGGGCCTTTCCTGTGCCAACCTGATGCCCCTTCCCCAAGAAGGTGAGTGCTTGTCATGGAAAATGTCCTGTGG TGACAGGCCCAGTGGAACAGTCACCCTTCTGGGCAAGGGGGAACAAATCACACCTCTGGGCTTCAGGGTATCCCAGACCCCTCTCAACACCCGCCCCCCC CATGTTTAAACTTTGTGCCTTTGACCATCTCTTAGGTCTAATGATATTTTATGCAAACAGTTCTTGGACCCCTGAATTCAATGACAGGGATGCCAACACC TTCTTGGCTTCTGGGACCTGTGTTCTTGCTGAGCACCCTCTCCGGTTTGGGTTGGGATAACAGAGGCAGGAGTGGCAGCTGTCCCCTCTCCCTGGGGATA TGCAACCCTTAGAGATTGCCCCAGAGCCCCACTCCCGGCCAGGCGGGAGATGGACCCCTCCCTTGCTCAGTGCCTCCTGGCCGGGGCCCCTCACCCCAAG GGGTCTGTATATACATTTCATAAGGCCTGCCCTCCCATGTTGCATGCCTATGTACTCTACGCCAAAGTGCAGCCCTTCCTCCTGAAGCCTCTGCCCTGCC TCCCTTTCTGGGAGGGCGGGGTGGGGGTGACTGAATTTGGGCCTCTTGTACAGTTAACTCTCCCAGGTGGATTTTGTGGAGGTGAGAAAAGGGGCATTGA GACTATAAAGCAGTAGACAATCCCCACATACCATCTGTAGAGTTGGAACTGCATTCTTTTAAAGTTTTATATGCATATATTTTAGGGCTGTAGACTTACT TTCCTATTTTCTTTTCCATTGCTTATTCTTGAGCACAAAATGATAATCAATTATTACATTTATACATCACCTTTTTGACTTTTCCAAGCCCTTTTACAGC TCTTGGCATTTTCCTCGCCTAGGCCTGTGAGGTAACTGGGATCGCACCTTTTATACCAGAGACCTGAGGCAGATGAAATTTATTTCCATCTAGGACTAGA AAAACTTGGGTCTCTTACCGCGAGACTGAGAGGCAGAAGTCAGCCCGAATGCCTGTCAGTTTCATGGAGGGGAAACGCAAAACCTGCAGTTCCTGAGTAC CTTCTACAGGCCCGGCCCAGCCTAGGCCCGGGGTGGCCACACCACAGCAAGCCGGCCCCCCCTCTTTTGGCCTTGTGGATAAGGGAGAGTTGACCGTTTT CATCCTGGCCTCCTTTTGCTGTTTGGATGTTTCCACGGGTCTCACTTATACCAAAGGGAAAACTCTTCATTAAAGTCCGTATTTCTTCTACCTCTGCCTC TTAACCCTTCTGGGAAGCCTATTGTGTACGCCGCGGGCCCTCAGCGGCAGACGGGGAGGCGGGAGGTCGCGGCC

SHC1.F7 (forward primer)
SHC1.R7 (reverse primer)
Single exon skipping

Frame 0

SSQPEKRLHVGRGWGLKLSGSELGSGSHLSLSL PRTSVTPGPQRSNQAKGLGIPPAWPPCPNWQGGQ AGQQPLFHLNYGSPAPQAQVQSTPE*VSVIAGG VT*NLLECQSLSPSPPAASTSWPQTANRSSPTT TCNLSHLHPAGIRTQPSMSPMLPKTL*IREPATF WSVPKGLPRMSSAPLARPSSCASNNTSGTHPNW SPLMTGWLALMAQHGMRRRKSHLTISTIMTSRG RNPPWGGW*T*GFGKEPLQGLLDPLHPMPRPPAT WELHCL*DSLLGEIQKSANRCHLHHPVQAESFL MIPPMSTSRT*TRPGKQWVVLGPPILLSMAVHP GTCLT*SPSKMLFACLHLPSRCPWLSSSEGSPGS MGS*AGGRLRHCCSSMGTSWYGRARPHLASMCS LACRVGSLSICYWWTLRVWFGLRITALKVSVTL SATTWTITCPSSLRAANCVYSNLWSGNCDLP*RS LPEDALQSFPPYSLTLGTSFGSVLWAWPCVRAG SSMDSGFHIQLSERV*VKSLGENPASPQTLITK VLMYRVAPHLGLSCANLMPLPQEGECLSWKMSCG DRPSGTVTLLGKGEQITPLGFRVSQTPLNTRPP HV*TLCL*PSLRSNDILCKQFLDP*IQ*QGCQH LLGFWDLCSC*APSPVWVGITEAGVAAVPSPWGY ATLRDCPRAPLPARREMDPSLAQCLLAGAPHPK GSVYTFHKACPPMLHAYVLYAKVQPFLLKPLPC LPFWEGGVGVTEFGPLVQLTLPGGFCGGEKRGIE TIKQ*TIPTYHL*SWNCILLKFYMHIF*GCRLT FLFSFPLLILEHKMIINYYIYTSPF*LFQALLQ LLAFSSPRPVR*LGSHLLYQRPEADEIYFHLGLE KLGSLTARLRGRSQPECLSVSWRGNAKPAVPEY LLQARPSLGPGWPHHSKPAPPLLALWIRES*PF SSWPPFAVWMFPRVSLIPKGKLFIKVRISSTSAS *PFWEAYCVRRGPSAADGEAGGRG

Frame 1

LLSQRRGSTLGGDGA*NCLGLSWGAEATCPSPS PGLL*LLGHRGPTRLRAWGYPLPGPLAQTGRGA RLGSSPSFTSTMDLLPPKPKYNPLRNESLSSLEE *PEICWNANHSHRLHQQPQPHGRRLQTDHRQPP HAIYLICIRRGSGHSRVCRLCCQRPCESESLPH SGVSRRACPGCHQHHWPGLRVALQTIPQEPTQTG HPS*QDGWL*WLSMG*GGGRAT*PSVL**LPGE GTPLGGGGRHEASGRSRSRGCSTHCTQCPDPQP LGSYIACRTACWGRSRSPQTDATSTTLSRQRAF* *SLLCQRPEPRQGPASSGWCWAPQSCYQWQCTP GPV*HEALRRCSSRASTSPVGVHG*AAPRGALV PWEAEPAGG*GTAAAQWGLPGTGEHDHTWPVCAH WLAEWAA*AFATGGP*GCGSD*GSPL*KCQSPY QLPHGQSLAHHLCGQRTVSTATCGAETVICPSA LFQKMPSNPFHPIP*LSGPRLGVFCGLGLVSELG VAWTLGFISS*VRGFESKAWVRILPLPKH*SPK Y*CTEWPLTWAFPVPT*CPFPKKVSACHGKCPV VTGPVEQSPFWARGNKSHLWASGYPRPLSTPAPP MFKLCAFDHLLGLMIFYANSSWTPEFNDRDANT FLASGTCVLAEHPLRFGLG*QRQEWQLSPLPGD MQPLEIAPEPHSRPGGRWTPPLLSASWPGPLTPR GLYIHFIRPALPCCMPMYSTPKCSPSS*SLCPA SLSGRAGWG*LNLGLLYS*LSQVDFVEVRKGAL RL*SSRQSPHTICRVGTAFF*SFICIYFRAVDLL SYFLFHCLFLSTK**SIITFIHHLFDFSKPFYS SWHFPRLGL*GNWDRTFYTRDLRQMKFISI*D* KNLGLLPRD*EAEVSPNACQFHGGETQNLQFLST FYRPGPA*ARGGHTTASRPPLFWPCG*GRVDRF HPGLLLLFGCFHGSHLYQRENSSLKSVFLLPLP LNPSGKPIVYAAGPQRQTGRREVAA

Frame 2

FSAREEAPRWAGMGPETVWV*AGERKPLVPLPP QDFCDSWATEVQPG*GPGDTPCLAPLPKLAGGP GWAAAPLSPQLWISCPPSPSTIHSGMSLCHRWR SNLKFAGMPITLTVSTSSLNLMAADCKQIIANHH MQSISFASGGDPDTAEYVAYVAKDPVNQRACHI LECPEGLAQDVISTIGQAFELRFKQYLRNPPKL VTPHDRMAGFDGSAWDEEEEEPPDHQYYNDFPGK EPPLGGVVDMRLREGAAPGAARPTAPNAQTPSH LGATLPVGQPVGGDPEVRKQMPPPPPCPGRELF DDPSYVNVQNLDKARQAVGGAGPPNPAINGSAPR DLFDMKPFEDALRVPPPPQSVSMAEQLRGEPWF HGKLSRREAEALLQLNGDFLVRESTTTPGQYVL TGLQSGQPKHLLLVDPEGVVRTKDHRFESVSHLI SYHMDNHLPIISAGSELCLQQPVERKL*SALAL SSRRCPPILSTLFPNSRDLVWECSVGLALCQSW E*HGLWVSYPAE*EGLSQKPG*ESCLSPNINHQS INVQSGPSPGPFLCQPDAPSPRR*VLVMENVLW *QAQWNSHPSGQGGTNHTSGLQGIPDPSQHPPP PCLNFVPLTIS*V**YFMQTVLGPLNSMTGMPTP SWLLGPVFLLSTLSGLGWDNRGRSGSCPLSLGI CNP*RLPQSPTPGQAGDGPLPCSVPPGRGPSPQ GVCIYIS*GLPSHVACLCTLRQSAALPPEASALP PFLGGRGGGD*IWASCTVNSPRWILWR*EKGH* DYKAVDNPHIPSVELELHSFKVLYAYILGL*TY FPIFFSIAYS*AQNDNQLLHLYITFLTFPSPFTA LGIFLA*ACEVTGIAPFIPET*GR*NLFPSRTR KTWVSYRETERQKSARMPVSFMEGKRKTCSS*V PSTGPAQPRPGVATPQQAGPPSFGLVDKGELTVF ILASFCCLDVSTGLTYTKGKTLH*SPYFFYLCL LTLLGSLLCTPRALSGRRGGGRSR

Sequence SHC1.fAug05

CCCAGTTGAGTGGGAGGCAGGCAGGCAGGCGCTGGGTCTGAGTACCCCTCTTTCCCCAGGGAGGCCATCAGTCTGGTGTGTGAGGCTGTGCCGGGTGCTA AGGGGGCGACAAGGAGGAGAAAGCCCTGTAGCCGCCCGCTCAGCTCTATCCTGGGGAGGAGTAACCTGAAATTTGCTGGAATGCCAATCACTCTCACCGT CTCCACCAGCAGCCTCAACCTCATGGCCGCAGACTGCAAACAGATCATCGCCAACCACCACATGCAATCTATCTCATTTGCATCCGGCGGGGATCCGGAC ACAGCCGAGTATGTCGCCTATGTTGCCAAAGACCCTGTGAATCAGAGAGCCTGCCACATTCTGGAGTGTCCCGAAGGGCTTGCCCAGGATGTCATCAGCA CCATTGGCCAGGCCTTCGAGTTGCGCTTCAAACAATACCTCAGGAACCCACCCAAACTGGTCACCCCTCATGACAGGATGGCTGGCTTTGATGGCTCAGC ATGGGATGAGGAGGAGGAAGAGCCACCTGACCATCAGTACTATAATGACTTCCCGGGGAAGGAACCCCCCTTGGGGGGGGTGGTAGACATGAGGCTTCGG GAAGGAGCCGCTCCAGGGGCTGCTCGACCCACTGCACCCAATGCCCAGACCCCCAGCCACTTGGGAGCTACATTGCCTGTAGGACAGCCTGTTGGGGGAG ATCCAGAAGTCCGCAAACAGATGCCACCTCCACCACCCTGTCCAGGCAGAGAGCTTTTTGATGATCCCTCCTATGTCAACGTCCAGAACCTAGACAAGGC CCGGCAAGCAGTGGGTGGTGCTGGGCCCCCCAATCCTGCTATCAATGGCAGTGCACCCCGGGACCTGTTTGACATGAAGCCCTTCGAAGATGCTCTTCGC GTGCCTCCACCTCCCCAGTCGGTGTCCATGGCTGAGCAGCTCCGAGGGGAGCCCTGGTTCCATGGGAAGCTGAGCCGGCGGGAGGCTGAGGCACTGCTGC AGCTCAATGGGGACTTCCTGGTACGGGAGAGCACGACCACACCTGGCCAGTATGTGCTCACTGGCTTGCAGAGTGGGCAGCCTAAGCATTTGCTACTGGT GGACCCTGAGGGTGTGGTGAGTGTGGCAGAGGTGTGGGTGAGGGGTGGCAAAAAGGAAGGTACAGTACCCTACAGTGTATCCCTCTTCCTTCCCTCATTG CTTTGTAGACATTCCCGCTGGGCAGCTGTCTATGGGCCTCTCTTGCTCTTTAAAGTGTCTGGCATCTTCCTCCAGAGGCTCATCCCTTAGCCTGAACTAT GTTCTTTATAGTCCTGGCACAGGGCCAGGCGTGGTGGCTCACACCTGTAATCCCAGCGCTTTGGGAGGCTGAGGCAGGTGAATCACCTGAGGTCAGGAGT TCGAGACCAGCCTGACCAATATGGTGAAACCCTGTCTCTACTAAAAATACAAAAATTAGCCGGGTGTGGTGGCAGGCACCTGTAGTCCCAGCTACTCAGG AGGCTGAGGCAGGAGAATTGCTTGAATCTGGGATGCAGAGGTTGCAATGAGCTGAGATCACACCACTGCACTCCAGCCTGGGTGACAGAGTGAGACTCTG TCTGAAAAAACAAAACAAAACAAATACAGTCCTGGCCCGTCCTCAAATCCCCAAGCCTTAAGCTTATCAGAGCTAGAAGGCAGTCATCTCATCTGTGCCC CTCTTTTGACAAATAAGGAAACCACCCTGAAGAGGTTGTGATGCCCAATGTCAGATGGTTAGAGGCTAGACACTGCAAGTTTCCTTTTGTAAGAAGATCC AAGGGCTTCTCCAGCTCCCCAGACTCAGGACTCAGACCACTGTGCTTTCTCTTATCTGCACTGCCTCACTGTTCTTCAAAACTTTCCTTGTTTAGCCAGG TGTGGTGGTTCATGCCTGTAATCCCAACATTTTGCGAAGCTGAGGTGGGAGGATTGCTTGAGGCCAGGAGTTCAAGACCAGCCTGGGCAACATAGCGAGA CCTTGTCTCTAGTCAGGAATGGTGACACATGCCTGTAGTCCTACCTACCCAGGAGGCTGAGGCCTGAGGATCGCTTGAGCCCAGGAGTTCGAGGTTACAG TGAGCCATGATTGCGCCACTGTACTCTAGCCTGGGTGACAGAGACCCTGTCTGGAAACAAAAAAACTTTTGTTTGTTTTCTACAGTAATCTCAGCTTCCT TTCTTTTTTTTTGAGATAGGGTTTTGCTTTGTCACTCAGGCTGCGGTGTGGTGGCACACCTGGCTAATTTTTGTATTTTTTTTTTGGTAGAGATGGGGTT TCGCCATGTTGCCCAAGCTGTTCTTGAACTCCCGGGCTCAAGTGATCCGCCTCCCTCGGCCTCCCAGAGTGCTGGGATTACAGGCACGGACCACCATGCC CAGCCTCCACATCTTTTTTTGCACTGTGTATACTCTTCTGAGACATGCCAACTTCCTCCAGGTCAAGAAAGGGGTATATAGCTCTCAGCTTCACTCTTTC AGGGCTGATGTCGCCTTTGCCTTTTCTCACTTCACTGACCTGTCTATTCCTACAACTGTCTCTTTCTAGAGAAGCCTCAATGATCAGGATTGACAGGCCA CACTCTCCCCCACCATTTTTTTCTCCTCCTTCAAGCCTCTTGTCTGTTTCACCCTCTTCCACCTTGGAGGCTGAGGTCTTATTTGACTCTTCACCTGAAT TGACCTTCTTCCTTCCCACCCTCCCAGGTTCGGACTAAGGATCACCGCTTTGAAAGTGTCAGTCACCTTATCAGCTACCACATGGACAATCACTTGCCCA TCATCTCTGCGGGCAGCGAACTGTGTCTACAGCAACCTGTGGAGCGGAAACTGTGATCTGCCCTAGCGCTCTCTTCCAGAAGATGCCCTCCAATCCTTTC CACCCTATTCCCTAACTCTCGGGACCTCGTTTGGGAGTGTTCTGTGGGCTTGGCCTTGTGTCAGAGCTGGGAGTAGCATGGACTCTGGGTTTCATATCCA GCTGAGTGAGAGGGTTTGAGTCAAAAGCCTGGGTGAGAATCCTGCCTCTCCCCAAACATTAATCACCAAAGTATTAATGTACAGAGTGGCCCCTCACCTG GGCCTTTCCTGTGCCAACCTGATGCCCCTTCCCCAAGAAGGTGAGTGCTTGTCATGGAAAATGTCCTGTGGTGACAGGCCCAGTGGAACAGTCACCCTTC TGGGCAAGGGGGAACAAATCACACCTCTGGGCTTCAGGGTATCCCAGACCCCTCTCAACACCCGCCCCCCCCATGTTTAAACTTTGTGCCTTTGACCATC TCTTAGGTCTAATGATATTTTATGCAAACAGTTCTTGGACCCCTGAATTCAATGACAGGGATGCCAACACCTTCTTGGCTTCTGGGACCTGTGTTCTTGC TGAGCACCCTCTCCGGTTTGGGTTGGGATAACAGAGGCAGGAGTGGCAGCTGTCCCCTCTCCCTGGGGATATGCAACCCTTAGAGATTGCCCCAGAGCCC CACTCCCGGCCAGGCGGGAGATGGACCCCTCCCTTGCTCAGTGCCTCCTGGCCGGGGCCCCTCACCCCAAGGGGTCTGTATATACATTTCATAAGGCCTG CCCTCCCATGTTGCATGCCTATGTACTCTACGCCAAAGTGCAGCCCTTCCTCCTGAAGCCTCTGCCCTGCCTCCCTTTCTGGGAGGGCGGGGTGGGGGTG ACTGAATTTGGGCCTCTTGTACAGTTAACTCTCCCAGGTGGATTTTGTGGAGGTGAGAAAAGGGGCATTGAGACTATAAAGCAGTAGACAATCCCCACAT ACCATCTGTAGAGTTGGAACTGCATTCTTTTAAAGTTTTATATGCATATATTTTAGGGCTGTAGACTTACTTTCCTATTTTCTTTTCCATTGCTTATTCT TGAGCACAAAATGATAATCAATTATTACATTTATACATCACCTTTTTGACTTTTCCAAGCCCTTTTACAGCTCTTGGCATTTTCCTCGCCTAGGCCTGTG AGGTAACTGGGATCGCACCTTTTATACCAGAGACCTGAGGCAGATGAAATTTATTTCCATCTAGGACTAGAAAAACTTGGGTCTCTTACCGCGAGACTGA GAGGCAGAAGTCAGCCCGAATGCCTGTCAGTTTCATGGAGGGGAAACGCAAAACCTGCAGTTCCTGAGTACCTTCTACAGGCCCGGCCCAGCCTAGGCCC GGGGTGGCCACACCACAGCAAGCCGGCCCCCCCTCTTTTGGCCTTGTGGATAAGGGAGAGTTGACCGTTTTCATCCTGGCCTCCTTTTGCTGTTTGGATG TTTCCACGGGTCTCACTTATACCAAAGGGAAAACTCTTCATTAAAGTCCGTATTTCTTCT

SHC1.F7 (forward primer)
SHC1.R7 (reverse primer)
Single exon skipping

Frame 0

PS*VGGRQAGAGSEYPSFPREAISLVCEAVPGA KGATRRRKPCSRPLSSILGRSNLKFAGMPITLTV STSSLNLMAADCKQIIANHHMQSISFASGGDPD TAEYVAYVAKDPVNQRACHILECPEGLAQDVIS TIGQAFELRFKQYLRNPPKLVTPHDRMAGFDGSA WDEEEEEPPDHQYYNDFPGKEPPLGGVVDMRLR EGAAPGAARPTAPNAQTPSHLGATLPVGQPVGG DPEVRKQMPPPPPCPGRELFDDPSYVNVQNLDKA RQAVGGAGPPNPAINGSAPRDLFDMKPFEDALR VPPPPQSVSMAEQLRGEPWFHGKLSRREAEALL QLNGDFLVRESTTTPGQYVLTGLQSGQPKHLLLV DPEGVVSVAEVWVRGGKKEGTVPYSVSLFLPSL LCRHSRWAAVYGPLLLFKVSGIFLQRLIP*PEL CSL*SWHRARRGGSHL*SQRFGRLRQVNHLRSGV RDQPDQYGETLSLLKIQKLAGCGGRHL*SQLLR RLRQENCLNLGCRGCNELRSHHCTPAWVTE*DS V*KNKTKQIQSWPVLKSPSLKLIRARRQSSHLCP SFDK*GNHPEEVVMPNVRWLEARHCKFPFVRRS KGFSSSPDSGLRPLCFLLSALPHCSSKLSLFSQ VWWFMPVIPTFCEAEVGGLLEARSSRPAWAT*RD LVSSQEW*HMPVVLPTQEAEA*GSLEPRSSRLQ *AMIAPLYSSLGDRDPVWKQKNFCLFSTVISAS FLFF*DRVLLCHSGCGVVAHLANFCIFFLVEMGF RHVAQAVLELPGSSDPPPSASQSAGITGTDHHA QPPHLFLHCVYSSETCQLPPGQERGI*LSASLF QG*CRLCLFSLH*PVYSYNCLFLEKPQ*SGLTGH TLPHHFFLLLQASCLFHPLPPWRLRSYLTLHLN *PSSFPPSQVRTKDHRFESVSHLISYHMDNHLP IISAGSELCLQQPVERKL*SALALSSRRCPPILS TLFPNSRDLVWECSVGLALCQSWE*HGLWVSYP AE*EGLSQKPG*ESCLSPNINHQSINVQSGPSP GPFLCQPDAPSPRR*VLVMENVLW*QAQWNSHPS GQGGTNHTSGLQGIPDPSQHPPPPCLNFVPLTI S*V**YFMQTVLGPLNSMTGMPTPSWLLGPVFL LSTLSGLGWDNRGRSGSCPLSLGICNP*RLPQSP TPGQAGDGPLPCSVPPGRGPSPQGVCIYIS*GL PSHVACLCTLRQSAALPPEASALPPFLGGRGGG D*IWASCTVNSPRWILWR*EKGH*DYKAVDNPHI PSVELELHSFKVLYAYILGL*TYFPIFFSIAYS *AQNDNQLLHLYITFLTFPSPFTALGIFLA*AC EVTGIAPFIPET*GR*NLFPSRTRKTWVSYRETE RQKSARMPVSFMEGKRKTCSS*VPSTGPAQPRP GVATPQQAGPPSFGLVDKGELTVFILASFCCLD VSTGLTYTKGKTLH*SPYFF

Frame 1

PVEWEAGRQALGLSTPLSPGRPSVWCVRLCRVL RGRQGGESPVAARSALSWGGVT*NLLECQSLSP SPPAASTSWPQTANRSSPTTTCNLSHLHPAGIRT QPSMSPMLPKTL*IREPATFWSVPKGLPRMSSA PLARPSSCASNNTSGTHPNWSPLMTGWLALMAQ HGMRRRKSHLTISTIMTSRGRNPPWGGW*T*GFG KEPLQGLLDPLHPMPRPPATWELHCL*DSLLGE IQKSANRCHLHHPVQAESFLMIPPMSTSRT*TR PGKQWVVLGPPILLSMAVHPGTCLT*SPSKMLFA CLHLPSRCPWLSSSEGSPGSMGS*AGGRLRHCC SSMGTSWYGRARPHLASMCSLACRVGSLSICYW WTLRVW*VWQRCG*GVAKRKVQYPTVYPSSFPHC FVDIPAGQLSMGLSCSLKCLASSSRGSSLSLNY VLYSPGTGPGVVAHTCNPSALGG*GR*IT*GQE FETSLTNMVKPCLY*KYKN*PGVVAGTCSPSYSG G*GRRIA*IWDAEVAMS*DHTTALQPG*QSETL SEKTKQNKYSPGPSSNPQALSLSELEGSHLICA PLLTNKETTLKRL*CPMSDG*RLDTASFLL*EDP RASPAPQTQDSDHCAFSYLHCLTVLQNFPCLAR CGGSCL*SQHFAKLRWEDCLRPGVQDQPGQHSE TLSLVRNGDTCL*SYLPRRLRPEDRLSPGVRGYS EP*LRHCTLAWVTETLSGNKKTFVCFLQ*SQLP FFFFEIGFCFVTQAAVWWHTWLIFVFFFW*RWG FAMLPKLFLNSRAQVIRLPRPPRVLGLQARTTMP SLHIFFCTVYTLLRHANFLQVKKGVYSSQLHSF RADVAFAFSHFTDLSIPTTVSF*RSLNDQD*QA TLSPTIFFSSFKPLVCFTLFHLGG*GLI*LFT*I DLLPSHPPRFGLRITALKVSVTLSATTWTITCP SSLRAANCVYSNLWSGNCDLP*RSLPEDALQSF PPYSLTLGTSFGSVLWAWPCVRAGSSMDSGFHIQ LSERV*VKSLGENPASPQTLITKVLMYRVAPHL GLSCANLMPLPQEGECLSWKMSCGDRPSGTVTL LGKGEQITPLGFRVSQTPLNTRPPHV*TLCL*PS LRSNDILCKQFLDP*IQ*QGCQHLLGFWDLCSC *APSPVWVGITEAGVAAVPSPWGYATLRDCPRA PLPARREMDPSLAQCLLAGAPHPKGSVYTFHKAC PPMLHAYVLYAKVQPFLLKPLPCLPFWEGGVGV TEFGPLVQLTLPGGFCGGEKRGIETIKQ*TIPT YHL*SWNCILLKFYMHIF*GCRLTFLFSFPLLIL EHKMIINYYIYTSPF*LFQALLQLLAFSSPRPV R*LGSHLLYQRPEADEIYFHLGLEKLGSLTARL RGRSQPECLSVSWRGNAKPAVPEYLLQARPSLGP GWPHHSKPAPPLLALWIRES*PFSSWPPFAVWM FPRVSLIPKGKLFIKVRISS

Frame 2

QLSGRQAGRRWV*VPLFPQGGHQSGV*GCAGC* GGDKEEKAL*PPAQLYPGEE*PEICWNANHSHR LHQQPQPHGRRLQTDHRQPPHAIYLICIRRGSG HSRVCRLCCQRPCESESLPHSGVSRRACPGCHQH HWPGLRVALQTIPQEPTQTGHPS*QDGWL*WLS MG*GGGRAT*PSVL**LPGEGTPLGGGGRHEAS GRSRSRGCSTHCTQCPDPQPLGSYIACRTACWGR SRSPQTDATSTTLSRQRAF**SLLCQRPEPRQG PASSGWCWAPQSCYQWQCTPGPV*HEALRRCSS RASTSPVGVHG*AAPRGALVPWEAEPAGG*GTAA AQWGLPGTGEHDHTWPVCAHWLAEWAA*AFATG GP*GCGECGRGVGEGWQKGRYSTLQCIPLPSLI AL*TFPLGSCLWASLAL*SVWHLPPEAHPLA*TM FFIVLAQGQAWWLTPVIPALWEAEAGESPEVRS SRPA*PIW*NPVSTKNTKISRVWWQAPVVPATQ EAEAGELLESGMQRLQ*AEITPLHSSLGDRVRLC LKKQNKTNTVLARPQIPKP*AYQS*KAVISSVP LF*QIRKPP*RGCDAQCQMVRG*TLQVSFCKKI QGLLQLPRLRTQTTVLSLICTASLFFKTFLV*PG VVVHACNPNILRS*GGRIA*GQEFKTSLGNIAR PCL*SGMVTHACSPTYPGG*GLRIA*AQEFEVT VSHDCATVL*PG*QRPCLETKKLLFVFYSNLSFL SFFLR*GFALSLRLRCGGTPG*FLYFFFGRDGV SPCCPSCS*TPGLK*SASLGLPECWDYRHGPPC PASTSFFALCILF*DMPTSSRSRKGYIALSFTLS GLMSPLPFLTSLTCLFLQLSLSREASMIRIDRP HSPPPFFSPPSSLLSVSPSSTLEAEVLFDSSPE LTFFLPTLPGSD*GSPL*KCQSPYQLPHGQSLAH HLCGQRTVSTATCGAETVICPSALFQKMPSNPF HPIP*LSGPRLGVFCGLGLVSELGVAWTLGFIS S*VRGFESKAWVRILPLPKH*SPKY*CTEWPLTW AFPVPT*CPFPKKVSACHGKCPVVTGPVEQSPF WARGNKSHLWASGYPRPLSTPAPPMFKLCAFDH LLGLMIFYANSSWTPEFNDRDANTFLASGTCVLA EHPLRFGLG*QRQEWQLSPLPGDMQPLEIAPEP HSRPGGRWTPPLLSASWPGPLTPRGLYIHFIRP ALPCCMPMYSTPKCSPSS*SLCPASLSGRAGWG* LNLGLLYS*LSQVDFVEVRKGALRL*SSRQSPH TICRVGTAFF*SFICIYFRAVDLLSYFLFHCLF LSTK**SIITFIHHLFDFSKPFYSSWHFPRLGL* GNWDRTFYTRDLRQMKFISI*D*KNLGLLPRD* EAEVSPNACQFHGGETQNLQFLSTFYRPGPA*A RGGHTTASRPPLFWPCG*GRVDRFHPGLLLLFGC FHGSHLYQRENSSLKSVFL

Sequence SHC1.eAug05

AGCGTGAAGGAGGGACCCTGAGGTAGAGGGTCAGGGGTTAGTGAGGCCGGAAGTGAGTGTAATAAAGTTTCTCCAGGGAGGCAGGGCCCGGGGAGAAAGT TGGAGCGGTAACCTAAGCTGGCAGTGGCGTGATCCGGCACCAAATCGGCCCGCGGTGCGGTGCGGAGACTCCATGAGGCCCTGGACATGAACAAGCTGAG TGGAGGCGGCGGGCGCAGGACTCGGGTGGAAGGGGGCCAGCTTGGGGGCGAGGAGTGGACCCGCCACGGGAGCTTTGTCAATAAGCCCACGCGGGGCTGG CTGCATCCCAACGACAAAGTCATGGGACCCGGGGTTTCCTACTTGGTTCGGTACATGGGTTGTGTGGAGGTCCTCCAGTCAATGCGTGCCCTGGACTTCA ACACCCGGACTCAGGTCACCAGGGAGGCCATCAGTCTGGTGTGTGAGGCTGTGCCGGGTGCTAAGGGGGCGACAAGGAGGAGAAAGCCCTGTAGCCGCCC GCTCAGCTCTATCCTGGGGAGGAGTAACCTGAAATTTGCTGGAATGCCAATCACTCTCACCGTCTCCACCAGCAGCCTCAACCTCATGGCCGCAGACTGC AAACAGATCATCGCCAACCACCACATGCAATCTATCTCATTTGCATCCGGCGGGGATCCGGACACAGCCGAGTATGTCGCCTATGTTGCCAAAGACCCTG TGAATCAGAGAGCCTGCCACATTCTGGAGTGTCCCGAAGGGCTTGCCCAGGATGTCATCAGCACCATTGGCCAGGCCTTCGAGTTGCGCTTCAAACAATA CCTCAGGAACCCACCCAAACTGGTCACCCCTCATGACAGGATGGCTGGCTTTGATGGCTCAGCATGGGATGAGGAGGAGGAAGAGCCACCTGACCATCAG TACTATAATGACTTCCCGGGGAAGGAACCCCCCTTGGGGGGGGTGGTAGACATGAGGCTTCGGGAAGGAGCCGCTCCAGGGGCTGCTCGACCCACTGCAC CCAATGCCCAGACCCCCAGCCACTTGGGAGCTACATTGCCTGTAGGACAGCCTGTTGGGGGAGATCCAGAAGTCCGCAAACAGATGCCACCTCCACCACC CTGTCCAGGCAGAGAGCTTTTTGATGATCCCTCCTATGTCAACGTCCAGAACCTAGACAAGGCCCGGCAAGCAGTGGGTGGTGCTGGGCCCCCCAATCCT GCTATCAATGGCAGTGCACCCCGGGACCTGTTTGACATGAAGCCCTTCGAAGATGCTCTTCGCGTGCCTCCACCTCCCCAGTCGGTGTCCATGGCTGAGC AGCTCCGAGGGGAGCCCTGGTTCCATGGGAAGCTGAGCCGGCGGGAGGCTGAGGCACTGCTGCAGCTCAATGGGGACTTCCTGGTACGGGAGAGCACGAC CACACCTGGCCAGTATGTGCTCACTGGCTTGCAGAGTGGGCAGCCTAAGCATTTGCTACTGGTGGACCCTGAGGGTGTGGTTCGGACTAAGGATCACCGC TTTGAAAGTGTCAGTCACCTTATCAGCTACCACATGGACAATCACTTGCCCATCATCTCTGCGGGCAGCGAACTGTGTCTACAGCAACCTGTGGAGCGGA AACTGTGATCTGCCCTAGCGCTCTCTTCCAGAAGATGCCCTCCAATCCTTTCCACCCTATTCCCTAACTCTCGGGACCTCGTTTGGGAGTGTTCTGTGGG CTTGGCCTTGTGTCAGAGCTGGGAGTAGCATGGACTCTGGGTTTCATATCCAGCTGAGTGAGAGGGTTTGAGTCAAAAGCCTGGGTGAGAATCCTGCCTC TCCCCAAACATTAATCACCAAAGTATTAATGTACAGAGTGGCCCCTCACCTGGGCCTTTCCTGTGCCAACCTGATGCCCCTTCCCCAAGAAGGTGAGTGC TTGTCATGGAAAATGTCCTGTGGTGACAGGCCCAGTGGAACAGTCACCCTTCTGGGCAAGGGGGAACAAATCACACCTCTGGGCTTCAGGGTATCCCAGA CCCCTCTCAACACCCGCCCCCCCCATGTTTAAACTTTGTGCCTTTGACCATCTCTTAGGTCTAATGATATTTTATGCAAACAGTTCTTGGACCCCTGAAT TCAATGACAGGGATGCCAACACCTTCTTGGCTTCTGGGACCTGTGTTCTTGCTGAGCACCCTCTCCGGTTTGGGTTGGGATAACAGAGGCAGGAGTGGCA GCTGTCCCCTCTCCCTGGGGATATGCAACCCTTAGAGATTGCCCCAGAGCCCCACTCCCGGCCAGGCGGGAGATGGACCCCTCCCTTGCTCAGTGCCTCC TGGCCGGGGCCCCTCACCCCAAGGGGTCTGTATATACATTTCATAAGGCCTGCCCTCCCATGTTGCATGCCTATGTACTCTACGCCAAAGTGCAGCCCTT CCTCCTGAAGCCTCTGCCCTGCCTCCCTTTCTGGGAGGGCGGGGTGGGGGTGACTGAATTTGGGCCTCTTGTACAGTTAACTCTCCCAGGTGGATTTTGT GGAGGTGAGAAAAGGGGCATTGAGACTATAAAGCAGTAGACAATCCCCACATACCATCTGTAGAGTTGGAACTGCATTCTTTTAAAGTTTTATATGCATA TATTTTAGGGCTGTAGACTTACTTTCCTATTTTCTTTTCCATTGCTTATTCTTGAGCACAAAATGATAATCAATTATTACATTTATACATCACCTTTTTG ACTTTTCCAAGCCCTTTTACAGCTCTTGGCATTTTCCTCGCCTAGGCCTGTGAGGTAACTGGGATCGCACCTTTTATACCAGAGACCTGAGGCAGATGAA ATTTATTTCCATCTAGGACTAGAAAAACTTGGGTCTCTTACCGCGAGACTGAGAGGCAGAAGTCAGCCCGAATGCCTGTCAGTTTCATGGAGGGGAAACG CAAAACCTGCAGTTCCTGAGTACCTTCTACAGGCCCGGCCCAGCCTAGGCCCGGGGTGGCCACACCACAGCAAGCCGGCCCCCCCTCTTTTGGCCTTGTG GATAAGGGAGAGTTGACCGTTTTCATCCTGGCCTCCTTTTGCTGTTTGGATGTTTCCACGGGTCTCACTTATACCAAAGGGAAAACTCTTCATTAAAGTC CGTATTTCTTCTACCTCTGCCTCTTAACCCTTCTGGGAAGCCTATTGTGTACGCCGCGGGCCCTCAGCGGCAGACGGGGAGGCGGGAGGTCGCGGCC

SHC1.F7 (forward primer)
SHC1.R7 (reverse primer)
Single exon skipping

Frame 0

SVKEGP*GRGSGVSEAGSECNKVSPGRQGPGRK LER*PKLAVA*SGTKSARGAVRRLHEALDMNKLS GGGGRRTRVEGGQLGGEEWTRHGSFVNKPTRGW LHPNDKVMGPGVSYLVRYMGCVEVLQSMRALDF NTRTQVTREAISLVCEAVPGAKGATRRRKPCSRP LSSILGRSNLKFAGMPITLTVSTSSLNLMAADC KQIIANHHMQSISFASGGDPDTAEYVAYVAKDP VNQRACHILECPEGLAQDVISTIGQAFELRFKQY LRNPPKLVTPHDRMAGFDGSAWDEEEEEPPDHQ YYNDFPGKEPPLGGVVDMRLREGAAPGAARPTA PNAQTPSHLGATLPVGQPVGGDPEVRKQMPPPPP CPGRELFDDPSYVNVQNLDKARQAVGGAGPPNP AINGSAPRDLFDMKPFEDALRVPPPPQSVSMAE QLRGEPWFHGKLSRREAEALLQLNGDFLVRESTT TPGQYVLTGLQSGQPKHLLLVDPEGVVRTKDHR FESVSHLISYHMDNHLPIISAGSELCLQQPVER KL*SALALSSRRCPPILSTLFPNSRDLVWECSVG LALCQSWE*HGLWVSYPAE*EGLSQKPG*ESCL SPNINHQSINVQSGPSPGPFLCQPDAPSPRR*V LVMENVLW*QAQWNSHPSGQGGTNHTSGLQGIPD PSQHPPPPCLNFVPLTIS*V**YFMQTVLGPLN SMTGMPTPSWLLGPVFLLSTLSGLGWDNRGRSG SCPLSLGICNP*RLPQSPTPGQAGDGPLPCSVPP GRGPSPQGVCIYIS*GLPSHVACLCTLRQSAAL PPEASALPPFLGGRGGGD*IWASCTVNSPRWIL WR*EKGH*DYKAVDNPHIPSVELELHSFKVLYAY ILGL*TYFPIFFSIAYS*AQNDNQLLHLYITFL TFPSPFTALGIFLA*ACEVTGIAPFIPET*GR* NLFPSRTRKTWVSYRETERQKSARMPVSFMEGKR KTCSS*VPSTGPAQPRPGVATPQQAGPPSFGLV DKGELTVFILASFCCLDVSTGLTYTKGKTLH*S PYFFYLCLLTLLGSLLCTPRALSGRRGGGRSR

Frame 1

A*RRDPEVEGQGLVRPEVSVIKFLQGGRARGES WSGNLSWQWRDPAPNRPAVRCGDSMRPWT*TS* VEAAGAGLGWKGASLGARSGPATGALSISPRGAG CIPTTKSWDPGFPTWFGTWVVWRSSSQCVPWTS TPGLRSPGRPSVWCVRLCRVLRGRQGGESPVAA RSALSWGGVT*NLLECQSLSPSPPAASTSWPQTA NRSSPTTTCNLSHLHPAGIRTQPSMSPMLPKTL *IREPATFWSVPKGLPRMSSAPLARPSSCASNN TSGTHPNWSPLMTGWLALMAQHGMRRRKSHLTIS TIMTSRGRNPPWGGW*T*GFGKEPLQGLLDPLH PMPRPPATWELHCL*DSLLGEIQKSANRCHLHH PVQAESFLMIPPMSTSRT*TRPGKQWVVLGPPIL LSMAVHPGTCLT*SPSKMLFACLHLPSRCPWLS SSEGSPGSMGS*AGGRLRHCCSSMGTSWYGRAR PHLASMCSLACRVGSLSICYWWTLRVWFGLRITA LKVSVTLSATTWTITCPSSLRAANCVYSNLWSG NCDLP*RSLPEDALQSFPPYSLTLGTSFGSVLW AWPCVRAGSSMDSGFHIQLSERV*VKSLGENPAS PQTLITKVLMYRVAPHLGLSCANLMPLPQEGEC LSWKMSCGDRPSGTVTLLGKGEQITPLGFRVSQ TPLNTRPPHV*TLCL*PSLRSNDILCKQFLDP*I Q*QGCQHLLGFWDLCSC*APSPVWVGITEAGVA AVPSPWGYATLRDCPRAPLPARREMDPSLAQCL LAGAPHPKGSVYTFHKACPPMLHAYVLYAKVQPF LLKPLPCLPFWEGGVGVTEFGPLVQLTLPGGFC GGEKRGIETIKQ*TIPTYHL*SWNCILLKFYMH IF*GCRLTFLFSFPLLILEHKMIINYYIYTSPF* LFQALLQLLAFSSPRPVR*LGSHLLYQRPEADE IYFHLGLEKLGSLTARLRGRSQPECLSVSWRGN AKPAVPEYLLQARPSLGPGWPHHSKPAPPLLALW IRES*PFSSWPPFAVWMFPRVSLIPKGKLFIKV RISSTSAS*PFWEAYCVRRGPSAADGEAGGRG

Frame 2

REGGTLR*RVRG**GRK*V**SFSREAGPGEKV GAVT*AGSGVIRHQIGPRCGAETP*GPGHEQAE WRRRAQDSGGRGPAWGRGVDPPRELCQ*AHAGL AASQRQSHGTRGFLLGSVHGLCGGPPVNACPGLQ HPDSGHQGGHQSGV*GCAGC*GGDKEEKAL*PP AQLYPGEE*PEICWNANHSHRLHQQPQPHGRRL QTDHRQPPHAIYLICIRRGSGHSRVCRLCCQRPC ESESLPHSGVSRRACPGCHQHHWPGLRVALQTI PQEPTQTGHPS*QDGWL*WLSMG*GGGRAT*PS VL**LPGEGTPLGGGGRHEASGRSRSRGCSTHCT QCPDPQPLGSYIACRTACWGRSRSPQTDATSTT LSRQRAF**SLLCQRPEPRQGPASSGWCWAPQS CYQWQCTPGPV*HEALRRCSSRASTSPVGVHG*A APRGALVPWEAEPAGG*GTAAAQWGLPGTGEHD HTWPVCAHWLAEWAA*AFATGGP*GCGSD*GSP L*KCQSPYQLPHGQSLAHHLCGQRTVSTATCGAE TVICPSALFQKMPSNPFHPIP*LSGPRLGVFCG LGLVSELGVAWTLGFISS*VRGFESKAWVRILP LPKH*SPKY*CTEWPLTWAFPVPT*CPFPKKVSA CHGKCPVVTGPVEQSPFWARGNKSHLWASGYPR PLSTPAPPMFKLCAFDHLLGLMIFYANSSWTPE FNDRDANTFLASGTCVLAEHPLRFGLG*QRQEWQ LSPLPGDMQPLEIAPEPHSRPGGRWTPPLLSAS WPGPLTPRGLYIHFIRPALPCCMPMYSTPKCSP SS*SLCPASLSGRAGWG*LNLGLLYS*LSQVDFV EVRKGALRL*SSRQSPHTICRVGTAFF*SFICI YFRAVDLLSYFLFHCLFLSTK**SIITFIHHLF DFSKPFYSSWHFPRLGL*GNWDRTFYTRDLRQMK FISI*D*KNLGLLPRD*EAEVSPNACQFHGGET QNLQFLSTFYRPGPA*ARGGHTTASRPPLFWPC G*GRVDRFHPGLLLLFGCFHGSHLYQRENSSLKS VFLLPLPLNPSGKPIVYAAGPQRQTGRREVAA

Sequence SHC1.dAug05

AGCGTGAAGGAGGGACCCTGAGGTAGAGGGTCAGGGGTTAGTGAGGCCGGAAGTGAGTGTAATAAAGTTTCTCCAGGGAGGCAGGGCCCGGGGAGAAAGT TGGAGCGGTAACCTAAGCTGGCAGTGGCGTGATCCGGCACCAAATCGGCCCGCGGTGCGGTGCGGAGACTCCATGAGGCCCTGGACATGAACAAGCTGAG TGGAGGCGGCGGGCGCAGGACTCGGGTGGAAGGGGGCCAGCTTGGGGGCGAGGAGTGGACCCGCCACGGGAGCTTTGTCAATAAGCCCACGCGGGGCTGG CTGCATCCCAACGACAAAGTCATGGGACCCGGGGTTTCCTACTTGGTTCGGTACATGGGTTGTGTGGAGGTCCTCCAGTCAATGCGTGCCCTGGACTTCA ACACCCGGACTCAGGTCACCAGGGAGGCCATCAGTCTGGTGTGTGAGGCTGTGCCGGGTGCTAAGGGGGCGACAAGGAGGAGAAAGCCCTGTAGCCGCCC GCTCAGCTCTATCCTGGGGAGGAGTAACCTGAAATTTGCTGGAATGCCAATCACTCTCACCGTCTCCACCAGCAGCCTCAACCTCATGGCCGCAGACTGC AAACAGATCATCGCCAACCACCACATGCAATCTATCTCATTTGCATCCGGCGGGGATCCGGACACAGCCGAGTATGTCGCCTATGTTGCCAAAGACCCTG TGAATCAGAGAGCCTGCCACATTCTGGAGTGTCCCGAAGGGCTTGCCCAGGATGTCATCAGCACCATTGGCCAGGCCTTCGAGTTGCGCTTCAAACAATA CCTCAGGAACCCACCCAAACTGGTCACCCCTCATGACAGGATGGCTGGCTTTGATGGCTCAGCATGGGATGAGGAGGAGGAAGAGCCACCTGACCATCAG TACTATAATGACTTCCCGGGGAAGGAACCCCCCTTGGGGGGGGTGGTAGACATGAGGCTTCGGGAAGGAGCCGCTCCAGGGGCTGCTCGACCCACTGCAC CCAATGCCCAGACCCCCAGCCACTTGGGAGCTACATTGCCTGTAGGACAGCCTGTTGGGGGAGATCCAGAAGTCCGCAAACAGATGCCACCTCCACCACC CTGTCCAGCAGGCAGAGAGCTTTTTGATGATCCCTCCTATGTCAACGTCCAGAACCTAGACAAGGCCCGGCAAGCAGTGGGTGGTGCTGGGCCCCCCAAT CCTGCTATCAATGGCAGTGCACCCCGGGACCTGTTTGACATGAAGCCCTTCGAAGATGCTCTTCGCGTGCCTCCACCTCCCCAGTCGGTGTCCATGGCTG AGCAGCTCCGAGGGGAGCCCTGGTTCCATGGGAAGCTGAGCCGGCGGGAGGCTGAGGCACTGCTGCAGCTCAATGGGGACTTCCTGGTACGGGAGAGCAC GACCACACCTGGCCAGTATGTGCTCACTGGCTTGCAGAGTGGGCAGCCTAAGCATTTGCTACTGGTGGACCCTGAGGGTGTGGTTCGGACTAAGGATCAC CGCTTTGAAAGTGTCAGTCACCTTATCAGCTACCACATGGACAATCACTTGCCCATCATCTCTGCGGGCAGCGAACTGTGTCTACAGCAACCTGTGGAGC GGAAACTGTGATCTGCCCTAGCGCTCTCTTCCAGAAGATGCCCTCCAATCCTTTCCACCCTATTCCCTAACTCTCGGGACCTCGTTTGGGAGTGTTCTGT GGGCTTGGCCTTGTGTCAGAGCTGGGAGTAGCATGGACTCTGGGTTTCATATCCAGCTGAGTGAGAGGGTTTGAGTCAAAAGCCTGGGTGAGAATCCTGC CTCTCCCCAAACATTAATCACCAAAGTATTAATGTACAGAGTGGCCCCTCACCTGGGCCTTTCCTGTGCCAACCTGATGCCCCTTCCCCAAGAAGGTGAG TGCTTGTCATGGAAAATGTCCTGTGGTGACAGGCCCAGTGGAACAGTCACCCTTCTGGGCAAGGGGGAACAAATCACACCTCTGGGCTTCAGGGTATCCC AGACCCCTCTCAACACCCGCCCCCCCCATGTTTAAACTTTGTGCCTTTGACCATCTCTTAGGTCTAATGATATTTTATGCAAACAGTTCTTGGACCCCTG AATTCAATGACAGGGATGCCAACACCTTCTTGGCTTCTGGGACCTGTGTTCTTGCTGAGCACCCTCTCCGGTTTGGGTTGGGATAACAGAGGCAGGAGTG GCAGCTGTCCCCTCTCCCTGGGGATATGCAACCCTTAGAGATTGCCCCAGAGCCCCACTCCCGGCCAGGCGGGAGATGGACCCCTCCCTTGCTCAGTGCC TCCTGGCCGGGGCCCCTCACCCCAAGGGGTCTGTATATACATTTCATAAGGCCTGCCCTCCCATGTTGCATGCCTATGTACTCTACGCCAAAGTGCAGCC CTTCCTCCTGAAGCCTCTGCCCTGCCTCCCTTTCTGGGAGGGCGGGGTGGGGGTGACTGAATTTGGGCCTCTTGTACAGTTAACTCTCCCAGGTGGATTT TGTGGAGGTGAGAAAAGGGGCATTGAGACTATAAAGCAGTAGACAATCCCCACATACCATCTGTAGAGTTGGAACTGCATTCTTTTAAAGTTTTATATGC ATATATTTTAGGGCTGTAGACTTACTTTCCTATTTTCTTTTCCATTGCTTATTCTTGAGCACAAAATGATAATCAATTATTACATTTATACATCACCTTT TTGACTTTTCCAAGCCCTTTTACAGCTCTTGGCATTTTCCTCGCCTAGGCCTGTGAGGTAACTGGGATCGCACCTTTTATACCAGAGACCTGAGGCAGAT GAAATTTATTTCCATCTAGGACTAGAAAAACTTGGGTCTCTTACCGCGAGACTGAGAGGCAGAAGTCAGCCCGAATGCCTGTCAGTTTCATGGAGGGGAA ACGCAAAACCTGCAGTTCCTGAGTACCTTCTACAGGCCCGGCCCAGCCTAGGCCCGGGGTGGCCACACCACAGCAAGCCGGCCCCCCCTCTTTTGGCCTT GTGGATAAGGGAGAGTTGACCGTTTTCATCCTGGCCTCCTTTTGCTGTTTGGATGTTTCCACGGGTCTCACTTATACCAAAGGGAAAACTCTTCATTAAA GTCCGTATTTCTTCTACCTCTGCCTCTTAACCCTTCTGGGAAGCCTATTGTGTACGCCGCGGGCCCTCAGCGGCAGACGGGGAGGCGGGAGGTCGCGGCC

SHC1.F7 (forward primer)
SHC1.R7 (reverse primer)
Single exon skipping

Frame 0

SVKEGP*GRGSGVSEAGSECNKVSPGRQGPGRK LER*PKLAVA*SGTKSARGAVRRLHEALDMNKLS GGGGRRTRVEGGQLGGEEWTRHGSFVNKPTRGW LHPNDKVMGPGVSYLVRYMGCVEVLQSMRALDF NTRTQVTREAISLVCEAVPGAKGATRRRKPCSRP LSSILGRSNLKFAGMPITLTVSTSSLNLMAADC KQIIANHHMQSISFASGGDPDTAEYVAYVAKDP VNQRACHILECPEGLAQDVISTIGQAFELRFKQY LRNPPKLVTPHDRMAGFDGSAWDEEEEEPPDHQ YYNDFPGKEPPLGGVVDMRLREGAAPGAARPTA PNAQTPSHLGATLPVGQPVGGDPEVRKQMPPPPP CPAGRELFDDPSYVNVQNLDKARQAVGGAGPPN PAINGSAPRDLFDMKPFEDALRVPPPPQSVSMA EQLRGEPWFHGKLSRREAEALLQLNGDFLVREST TTPGQYVLTGLQSGQPKHLLLVDPEGVVRTKDH RFESVSHLISYHMDNHLPIISAGSELCLQQPVE RKL*SALALSSRRCPPILSTLFPNSRDLVWECSV GLALCQSWE*HGLWVSYPAE*EGLSQKPG*ESC LSPNINHQSINVQSGPSPGPFLCQPDAPSPRR* VLVMENVLW*QAQWNSHPSGQGGTNHTSGLQGIP DPSQHPPPPCLNFVPLTIS*V**YFMQTVLGPL NSMTGMPTPSWLLGPVFLLSTLSGLGWDNRGRS GSCPLSLGICNP*RLPQSPTPGQAGDGPLPCSVP PGRGPSPQGVCIYIS*GLPSHVACLCTLRQSAA LPPEASALPPFLGGRGGGD*IWASCTVNSPRWI LWR*EKGH*DYKAVDNPHIPSVELELHSFKVLYA YILGL*TYFPIFFSIAYS*AQNDNQLLHLYITF LTFPSPFTALGIFLA*ACEVTGIAPFIPET*GR *NLFPSRTRKTWVSYRETERQKSARMPVSFMEGK RKTCSS*VPSTGPAQPRPGVATPQQAGPPSFGL VDKGELTVFILASFCCLDVSTGLTYTKGKTLH* SPYFFYLCLLTLLGSLLCTPRALSGRRGGGRSR

Frame 1

A*RRDPEVEGQGLVRPEVSVIKFLQGGRARGES WSGNLSWQWRDPAPNRPAVRCGDSMRPWT*TS* VEAAGAGLGWKGASLGARSGPATGALSISPRGAG CIPTTKSWDPGFPTWFGTWVVWRSSSQCVPWTS TPGLRSPGRPSVWCVRLCRVLRGRQGGESPVAA RSALSWGGVT*NLLECQSLSPSPPAASTSWPQTA NRSSPTTTCNLSHLHPAGIRTQPSMSPMLPKTL *IREPATFWSVPKGLPRMSSAPLARPSSCASNN TSGTHPNWSPLMTGWLALMAQHGMRRRKSHLTIS TIMTSRGRNPPWGGW*T*GFGKEPLQGLLDPLH PMPRPPATWELHCL*DSLLGEIQKSANRCHLHH PVQQAESFLMIPPMSTSRT*TRPGKQWVVLGPPI LLSMAVHPGTCLT*SPSKMLFACLHLPSRCPWL SSSEGSPGSMGS*AGGRLRHCCSSMGTSWYGRA RPHLASMCSLACRVGSLSICYWWTLRVWFGLRIT ALKVSVTLSATTWTITCPSSLRAANCVYSNLWS GNCDLP*RSLPEDALQSFPPYSLTLGTSFGSVL WAWPCVRAGSSMDSGFHIQLSERV*VKSLGENPA SPQTLITKVLMYRVAPHLGLSCANLMPLPQEGE CLSWKMSCGDRPSGTVTLLGKGEQITPLGFRVS QTPLNTRPPHV*TLCL*PSLRSNDILCKQFLDP* IQ*QGCQHLLGFWDLCSC*APSPVWVGITEAGV AAVPSPWGYATLRDCPRAPLPARREMDPSLAQC LLAGAPHPKGSVYTFHKACPPMLHAYVLYAKVQP FLLKPLPCLPFWEGGVGVTEFGPLVQLTLPGGF CGGEKRGIETIKQ*TIPTYHL*SWNCILLKFYM HIF*GCRLTFLFSFPLLILEHKMIINYYIYTSPF *LFQALLQLLAFSSPRPVR*LGSHLLYQRPEAD EIYFHLGLEKLGSLTARLRGRSQPECLSVSWRG NAKPAVPEYLLQARPSLGPGWPHHSKPAPPLLAL WIRES*PFSSWPPFAVWMFPRVSLIPKGKLFIK VRISSTSAS*PFWEAYCVRRGPSAADGEAGGRG

Frame 2

REGGTLR*RVRG**GRK*V**SFSREAGPGEKV GAVT*AGSGVIRHQIGPRCGAETP*GPGHEQAE WRRRAQDSGGRGPAWGRGVDPPRELCQ*AHAGL AASQRQSHGTRGFLLGSVHGLCGGPPVNACPGLQ HPDSGHQGGHQSGV*GCAGC*GGDKEEKAL*PP AQLYPGEE*PEICWNANHSHRLHQQPQPHGRRL QTDHRQPPHAIYLICIRRGSGHSRVCRLCCQRPC ESESLPHSGVSRRACPGCHQHHWPGLRVALQTI PQEPTQTGHPS*QDGWL*WLSMG*GGGRAT*PS VL**LPGEGTPLGGGGRHEASGRSRSRGCSTHCT QCPDPQPLGSYIACRTACWGRSRSPQTDATSTT LSSRQRAF**SLLCQRPEPRQGPASSGWCWAPQ SCYQWQCTPGPV*HEALRRCSSRASTSPVGVHG* AAPRGALVPWEAEPAGG*GTAAAQWGLPGTGEH DHTWPVCAHWLAEWAA*AFATGGP*GCGSD*GS PL*KCQSPYQLPHGQSLAHHLCGQRTVSTATCGA ETVICPSALFQKMPSNPFHPIP*LSGPRLGVFC GLGLVSELGVAWTLGFISS*VRGFESKAWVRIL PLPKH*SPKY*CTEWPLTWAFPVPT*CPFPKKVS ACHGKCPVVTGPVEQSPFWARGNKSHLWASGYP RPLSTPAPPMFKLCAFDHLLGLMIFYANSSWTP EFNDRDANTFLASGTCVLAEHPLRFGLG*QRQEW QLSPLPGDMQPLEIAPEPHSRPGGRWTPPLLSA SWPGPLTPRGLYIHFIRPALPCCMPMYSTPKCS PSS*SLCPASLSGRAGWG*LNLGLLYS*LSQVDF VEVRKGALRL*SSRQSPHTICRVGTAFF*SFIC IYFRAVDLLSYFLFHCLFLSTK**SIITFIHHL FDFSKPFYSSWHFPRLGL*GNWDRTFYTRDLRQM KFISI*D*KNLGLLPRD*EAEVSPNACQFHGGE TQNLQFLSTFYRPGPA*ARGGHTTASRPPLFWP CG*GRVDRFHPGLLLLFGCFHGSHLYQRENSSLK SVFLLPLPLNPSGKPIVYAAGPQRQTGRREVAA

Sequence SHC1.bAug05

TCTTCTCAGCCAGAGAAGAGGCTCCACGTTGGGCGGGGATGGGGCCTGAAACTGTCTGGGTCTGAGCTGGGGAGCGGAAGCCACTTGTCCCTCTCCCTCC CCAGGACTTCTGTGACTCCTGGGCCACAGAGGTCCAACCAGGCTAAGGGCCTGGGGATACCCCCTGCCTGGCCCCCTTGCCCAAACTGGCAGGGGGGCCA GGCTGGGCAGCAGCCCCTCTTTCACCTCAACTATGGATCTCCTGCCCCCCAAGCCCAAGTACAATCCACTCCGGAATGAGTCTCTGTCATCGCTGGAGGA AGGGGCTTCTGGGTCCACCCCCCCGGAGGAGCTGCCTTCCCCATCAGCTTCATCCCTGGGGCCCATCCTGCCTCCTCTGCCTGGGGACGATAGTCCCACT ACCCTGTGCTCCTTCTTCCCCCGGATGAGCAACCTGAGGCTGGCCAACCCGGCTGGGGGGCGCCCAGGGTCTAAGGGGGAGCCAGGAAGGGCAGCTGATG ATGGGGAGGGGATCGTAGGGGCAGCCATGCCAGACTCAGGCCCCCTACCCCTCCTCCAGGACATGAACAAGCTGAGTGGAGGCGGCGGGCGCAGGACTCG GGTGGAAGGGGGCCAGCTTGGGGGCGAGGAGTGGACCCGCCACGGGAGCTTTGTCAATAAGCCCACGCGGGGCTGGCTGCATCCCAACGACAAAGTCATG GGACCCGGGGTTTCCTACTTGGTTCGGTACATGGGTTGTGTGGAGGTCCTCCAGTCAATGCGTGCCCTGGACTTCAACACCCGGACTCAGGTCACCAGGG AGGCCATCAGTCTGGTGTGTGAGGCTGTGCCGGGTGCTAAGGGGGCGACAAGGAGGAGAAAGCCCTGTAGCCGCCCGCTCAGCTCTATCCTGGGGAGGAG TAACCTGAAATTTGCTGGAATGCCAATCACTCTCACCGTCTCCACCAGCAGCCTCAACCTCATGGCCGCAGACTGCAAACAGATCATCGCCAACCACCAC ATGCAATCTATCTCATTTGCATCCGGCGGGGATCCGGACACAGCCGAGTATGTCGCCTATGTTGCCAAAGACCCTGTGAATCAGAGAGCCTGCCACATTC TGGAGTGTCCCGAAGGGCTTGCCCAGGATGTCATCAGCACCATTGGCCAGGCCTTCGAGTTGCGCTTCAAACAATACCTCAGGAACCCACCCAAACTGGT CACCCCTCATGACAGGATGGCTGGCTTTGATGGCTCAGCATGGGATGAGGAGGAGGAAGAGCCACCTGACCATCAGTACTATAATGACTTCCCGGGGAAG GAACCCCCCTTGGGGGGGGTGGTAGACATGAGGCTTCGGGAAGGAGCCGCTCCAGGGGCTGCTCGACCCACTGCACCCAATGCCCAGACCCCCAGCCACT TGGGAGCTACATTGCCTGTAGGACAGCCTGTTGGGGGAGATCCAGAAGTCCGCAAACAGATGCCACCTCCACCACCCTGTCCAGGCAGAGAGCTTTTTGA TGATCCCTCCTATGTCAACGTCCAGAACCTAGACAAGGCCCGGCAAGCAGTGGGTGGTGCTGGGCCCCCCAATCCTGCTATCAATGGCAGTGCACCCCGG GACCTGTTTGACATGAAGCCCTTCGAAGATGCTCTTCGCGTGCCTCCACCTCCCCAGTCGGTGTCCATGGCTGAGCAGCTCCGAGGGGAGCCCTGGTTCC ATGGGAAGCTGAGCCGGCGGGAGGCTGAGGCACTGCTGCAGCTCAATGGGGACTTCCTGGTACGGGAGAGCACGACCACACCTGGCCAGTATGTGCTCAC TGGCTTGCAGAGTGGGCAGCCTAAGCATTTGCTACTGGTGGACCCTGAGGGTGTGGTTCGGACTAAGGATCACCGCTTTGAAAGTGTCAGTCACCTTATC AGCTACCACATGGACAATCACTTGCCCATCATCTCTGCGGGCAGCGAACTGTGTCTACAGCAACCTGTGGAGCGGAAACTGTGATCTGCCCTAGCGCTCT CTTCCAGAAGATGCCCTCCAATCCTTTCCACCCTATTCCCTAACTCTCGGGACCTCGTTTGGGAGTGTTCTGTGGGCTTGGCCTTGTGTCAGAGCTGGGA GTAGCATGGACTCTGGGTTTCATATCCAGCTGAGTGAGAGGGTTTGAGTCAAAAGCCTGGGTGAGAATCCTGCCTCTCCCCAAACATTAATCACCAAAGT ATTAATGTACAGAGTGGCCCCTCACCTGGGCCTTTCCTGTGCCAACCTGATGCCCCTTCCCCAAGAAGGTGAGTGCTTGTCATGGAAAATGTCCTGTGGT GACAGGCCCAGTGGAACAGTCACCCTTCTGGGCAAGGGGGAACAAATCACACCTCTGGGCTTCAGGGTATCCCAGACCCCTCTCAACACCCGCCCCCCCC ATGTTTAAACTTTGTGCCTTTGACCATCTCTTAGGTCTAATGATATTTTATGCAAACAGTTCTTGGACCCCTGAATTCAATGACAGGGATGCCAACACCT TCTTGGCTTCTGGGACCTGTGTTCTTGCTGAGCACCCTCTCCGGTTTGGGTTGGGATAACAGAGGCAGGAGTGGCAGCTGTCCCCTCTCCCTGGGGATAT GCAACCCTTAGAGATTGCCCCAGAGCCCCACTCCCGGCCAGGCGGGAGATGGACCCCTCCCTTGCTCAGTGCCTCCTGGCCGGGGCCCCTCACCCCAAGG GGTCTGTATATACATTTCATAAGGCCTGCCCTCCCATGTTGCATGCCTATGTACTCTACGCCAAAGTGCAGCCCTTCCTCCTGAAGCCTCTGCCCTGCCT CCCTTTCTGGGAGGGCGGGGTGGGGGTGACTGAATTTGGGCCTCTTGTACAGTTAACTCTCCCAGGTGGATTTTGTGGAGGTGAGAAAAGGGGCATTGAG ACTATAAAGCAGTAGACAATCCCCACATACCATCTGTAGAGTTGGAACTGCATTCTTTTAAAGTTTTATATGCATATATTTTAGGGCTGTAGACTTACTT TCCTATTTTCTTTTCCATTGCTTATTCTTGAGCACAAAATGATAATCAATTATTACATTTATACATCACCTTTTTGACTTTTCCAAGCCCTTTTACAGCT CTTGGCATTTTCCTCGCCTAGGCCTGTGAGGTAACTGGGATCGCACCTTTTATACCAGAGACCTGAGGCAGATGAAATTTATTTCCATCTAGGACTAGAA AAACTTGGGTCTCTTACCGCGAGACTGAGAGGCAGAAGTCAGCCCGAATGCCTGTCAGTTTCATGGAGGGGAAACGCAAAACCTGCAGTTCCTGAGTACC TTCTACAGGCCCGGCCCAGCCTAGGCCCGGGGTGGCCACACCACAGCAAGCCGGCCCCCCCTCTTTTGGCCTTGTGGATAAGGGAGAGTTGACCGTTTTC ATCCTGGCCTCCTTTTGCTGTTTGGATGTTTCCACGGGTCTCACTTATACCAAAGGGAAAACTCTTCATTAAAGTCCGTATTTCTTCTACCTCTGCCTCT TAACCCTTCTGGGAAGCCTATTGTGTACGCCGCGGGCCCTCAGCGGCAGACGGGGAGGCGGGAGGTCGCGGCC

SHC1.F7 (forward primer)
SHC1.R7 (reverse primer)
Single exon skipping

Frame 0

SSQPEKRLHVGRGWGLKLSGSELGSGSHLSLSL PRTSVTPGPQRSNQAKGLGIPPAWPPCPNWQGGQ AGQQPLFHLNYGSPAPQAQVQSTPE*VSVIAGG RGFWVHPPGGAAFPISFIPGAHPASSAWGR*SH YPVLLLPPDEQPEAGQPGWGAPRV*GGARKGS** WGGDRRGSHARLRPPTPPPGHEQAEWRRRAQDS GGRGPAWGRGVDPPRELCQ*AHAGLAASQRQSH GTRGFLLGSVHGLCGGPPVNACPGLQHPDSGHQG GHQSGV*GCAGC*GGDKEEKAL*PPAQLYPGEE *PEICWNANHSHRLHQQPQPHGRRLQTDHRQPP HAIYLICIRRGSGHSRVCRLCCQRPCESESLPHS GVSRRACPGCHQHHWPGLRVALQTIPQEPTQTG HPS*QDGWL*WLSMG*GGGRAT*PSVL**LPGE GTPLGGGGRHEASGRSRSRGCSTHCTQCPDPQPL GSYIACRTACWGRSRSPQTDATSTTLSRQRAF* *SLLCQRPEPRQGPASSGWCWAPQSCYQWQCTP GPV*HEALRRCSSRASTSPVGVHG*AAPRGALVP WEAEPAGG*GTAAAQWGLPGTGEHDHTWPVCAH WLAEWAA*AFATGGP*GCGSD*GSPL*KCQSPY QLPHGQSLAHHLCGQRTVSTATCGAETVICPSAL FQKMPSNPFHPIP*LSGPRLGVFCGLGLVSELG VAWTLGFISS*VRGFESKAWVRILPLPKH*SPK Y*CTEWPLTWAFPVPT*CPFPKKVSACHGKCPVV TGPVEQSPFWARGNKSHLWASGYPRPLSTPAPP MFKLCAFDHLLGLMIFYANSSWTPEFNDRDANT FLASGTCVLAEHPLRFGLG*QRQEWQLSPLPGDM QPLEIAPEPHSRPGGRWTPPLLSASWPGPLTPR GLYIHFIRPALPCCMPMYSTPKCSPSS*SLCPA SLSGRAGWG*LNLGLLYS*LSQVDFVEVRKGALR L*SSRQSPHTICRVGTAFF*SFICIYFRAVDLL SYFLFHCLFLSTK**SIITFIHHLFDFSKPFYS SWHFPRLGL*GNWDRTFYTRDLRQMKFISI*D*K NLGLLPRD*EAEVSPNACQFHGGETQNLQFLST FYRPGPA*ARGGHTTASRPPLFWPCG*GRVDRF HPGLLLLFGCFHGSHLYQRENSSLKSVFLLPLPL NPSGKPIVYAAGPQRQTGRREVAA

Frame 1

LLSQRRGSTLGGDGA*NCLGLSWGAEATCPSPS PGLL*LLGHRGPTRLRAWGYPLPGPLAQTGRGA RLGSSPSFTSTMDLLPPKPKYNPLRNESLSSLEE GASGSTPPEELPSPSASSLGPILPPLPGDDSPT TLCSFFPRMSNLRLANPAGGRPGSKGEPGRAAD DGEGIVGAAMPDSGPLPLLQDMNKLSGGGGRRTR VEGGQLGGEEWTRHGSFVNKPTRGWLHPNDKVM GPGVSYLVRYMGCVEVLQSMRALDFNTRTQVTR EAISLVCEAVPGAKGATRRRKPCSRPLSSILGRS NLKFAGMPITLTVSTSSLNLMAADCKQIIANHH MQSISFASGGDPDTAEYVAYVAKDPVNQRACHI LECPEGLAQDVISTIGQAFELRFKQYLRNPPKLV TPHDRMAGFDGSAWDEEEEEPPDHQYYNDFPGK EPPLGGVVDMRLREGAAPGAARPTAPNAQTPSH LGATLPVGQPVGGDPEVRKQMPPPPPCPGRELFD DPSYVNVQNLDKARQAVGGAGPPNPAINGSAPR DLFDMKPFEDALRVPPPPQSVSMAEQLRGEPWF HGKLSRREAEALLQLNGDFLVRESTTTPGQYVLT GLQSGQPKHLLLVDPEGVVRTKDHRFESVSHLI SYHMDNHLPIISAGSELCLQQPVERKL*SALAL SSRRCPPILSTLFPNSRDLVWECSVGLALCQSWE *HGLWVSYPAE*EGLSQKPG*ESCLSPNINHQS INVQSGPSPGPFLCQPDAPSPRR*VLVMENVLW *QAQWNSHPSGQGGTNHTSGLQGIPDPSQHPPPP CLNFVPLTIS*V**YFMQTVLGPLNSMTGMPTP SWLLGPVFLLSTLSGLGWDNRGRSGSCPLSLGI CNP*RLPQSPTPGQAGDGPLPCSVPPGRGPSPQG VCIYIS*GLPSHVACLCTLRQSAALPPEASALP PFLGGRGGGD*IWASCTVNSPRWILWR*EKGH* DYKAVDNPHIPSVELELHSFKVLYAYILGL*TYF PIFFSIAYS*AQNDNQLLHLYITFLTFPSPFTA LGIFLA*ACEVTGIAPFIPET*GR*NLFPSRTR KTWVSYRETERQKSARMPVSFMEGKRKTCSS*VP STGPAQPRPGVATPQQAGPPSFGLVDKGELTVF ILASFCCLDVSTGLTYTKGKTLH*SPYFFYLCL LTLLGSLLCTPRALSGRRGGGRSR

Frame 2

FSAREEAPRWAGMGPETVWV*AGERKPLVPLPP QDFCDSWATEVQPG*GPGDTPCLAPLPKLAGGP GWAAAPLSPQLWISCPPSPSTIHSGMSLCHRWR KGLLGPPPRRSCLPHQLHPWGPSCLLCLGTIVPL PCAPSSPG*AT*GWPTRLGGAQGLRGSQEGQLM MGRGS*GQPCQTQAPYPSSRT*TS*VEAAGAGL GWKGASLGARSGPATGALSISPRGAGCIPTTKSW DPGFPTWFGTWVVWRSSSQCVPWTSTPGLRSPG RPSVWCVRLCRVLRGRQGGESPVAARSALSWGG VT*NLLECQSLSPSPPAASTSWPQTANRSSPTTT CNLSHLHPAGIRTQPSMSPMLPKTL*IREPATF WSVPKGLPRMSSAPLARPSSCASNNTSGTHPNW SPLMTGWLALMAQHGMRRRKSHLTISTIMTSRGR NPPWGGW*T*GFGKEPLQGLLDPLHPMPRPPAT WELHCL*DSLLGEIQKSANRCHLHHPVQAESFL MIPPMSTSRT*TRPGKQWVVLGPPILLSMAVHPG TCLT*SPSKMLFACLHLPSRCPWLSSSEGSPGS MGS*AGGRLRHCCSSMGTSWYGRARPHLASMCS LACRVGSLSICYWWTLRVWFGLRITALKVSVTLS ATTWTITCPSSLRAANCVYSNLWSGNCDLP*RS LPEDALQSFPPYSLTLGTSFGSVLWAWPCVRAG SSMDSGFHIQLSERV*VKSLGENPASPQTLITKV LMYRVAPHLGLSCANLMPLPQEGECLSWKMSCG DRPSGTVTLLGKGEQITPLGFRVSQTPLNTRPP HV*TLCL*PSLRSNDILCKQFLDP*IQ*QGCQHL LGFWDLCSC*APSPVWVGITEAGVAAVPSPWGY ATLRDCPRAPLPARREMDPSLAQCLLAGAPHPK GSVYTFHKACPPMLHAYVLYAKVQPFLLKPLPCL PFWEGGVGVTEFGPLVQLTLPGGFCGGEKRGIE TIKQ*TIPTYHL*SWNCILLKFYMHIF*GCRLT FLFSFPLLILEHKMIINYYIYTSPF*LFQALLQL LAFSSPRPVR*LGSHLLYQRPEADEIYFHLGLE KLGSLTARLRGRSQPECLSVSWRGNAKPAVPEY LLQARPSLGPGWPHHSKPAPPLLALWIRES*PFS SWPPFAVWMFPRVSLIPKGKLFIKVRISSTSAS *PFWEAYCVRRGPSAADGEAGGRG

Sequence SHC1.aAug05

TCTTCTCAGCCAGAGAAGAGGCTCCACGTTGGGCGGGGATGGGGCCTGAAACTGTCTGGGTCTGAGCTGGGGAGCGGAAGCCACTTGTCCCTCTCCCTCC CCAGGACTTCTGTGACTCCTGGGCCACAGAGGTCCAACCAGGCTAAGGGCCTGGGGATACCCCCTGCCTGGCCCCCTTGCCCAAACTGGCAGGGGGGCCA GGCTGGGCAGCAGCCCCTCTTTCACCTCAACTATGGATCTCCTGCCCCCCAAGCCCAAGTACAATCCACTCCGGAATGAGTCTCTGTCATCGCTGGAGGA AGGGGCTTCTGGGTCCACCCCCCCGGAGGAGCTGCCTTCCCCATCAGCTTCATCCCTGGGGCCCATCCTGCCTCCTCTGCCTGGGGACGATAGTCCCACT ACCCTGTGCTCCTTCTTCCCCCGGATGAGCAACCTGAGGCTGGCCAACCCGGCTGGGGGGCGCCCAGGGTCTAAGGGGGAGCCAGGAAGGGCAGCTGATG ATGGGGAGGGGATCGTAGGGGCAGCCATGCCAGACTCAGGCCCCCTACCCCTCCTCCAGGACATGAACAAGCTGAGTGGAGGCGGCGGGCGCAGGACTCG GGTGGAAGGGGGCCAGCTTGGGGGCGAGGAGTGGACCCGCCACGGGAGCTTTGTCAATAAGCCCACGCGGGGCTGGCTGCATCCCAACGACAAAGTCATG GGACCCGGGGTTTCCTACTTGGTTCGGTACATGGGTTGTGTGGAGGTCCTCCAGTCAATGCGTGCCCTGGACTTCAACACCCGGACTCAGGTCACCAGGG AGGCCATCAGTCTGGTGTGTGAGGCTGTGCCGGGTGCTAAGGGGGCGACAAGGAGGAGAAAGCCCTGTAGCCGCCCGCTCAGCTCTATCCTGGGGAGGAG TAACCTGAAATTTGCTGGAATGCCAATCACTCTCACCGTCTCCACCAGCAGCCTCAACCTCATGGCCGCAGACTGCAAACAGATCATCGCCAACCACCAC ATGCAATCTATCTCATTTGCATCCGGCGGGGATCCGGACACAGCCGAGTATGTCGCCTATGTTGCCAAAGACCCTGTGAATCAGAGAGCCTGCCACATTC TGGAGTGTCCCGAAGGGCTTGCCCAGGATGTCATCAGCACCATTGGCCAGGCCTTCGAGTTGCGCTTCAAACAATACCTCAGGAACCCACCCAAACTGGT CACCCCTCATGACAGGATGGCTGGCTTTGATGGCTCAGCATGGGATGAGGAGGAGGAAGAGCCACCTGACCATCAGTACTATAATGACTTCCCGGGGAAG GAACCCCCCTTGGGGGGGGTGGTAGACATGAGGCTTCGGGAAGGAGCCGCTCCAGGGGCTGCTCGACCCACTGCACCCAATGCCCAGACCCCCAGCCACT TGGGAGCTACATTGCCTGTAGGACAGCCTGTTGGGGGAGATCCAGAAGTCCGCAAACAGATGCCACCTCCACCACCCTGTCCAGCAGGCAGAGAGCTTTT TGATGATCCCTCCTATGTCAACGTCCAGAACCTAGACAAGGCCCGGCAAGCAGTGGGTGGTGCTGGGCCCCCCAATCCTGCTATCAATGGCAGTGCACCC CGGGACCTGTTTGACATGAAGCCCTTCGAAGATGCTCTTCGCGTGCCTCCACCTCCCCAGTCGGTGTCCATGGCTGAGCAGCTCCGAGGGGAGCCCTGGT TCCATGGGAAGCTGAGCCGGCGGGAGGCTGAGGCACTGCTGCAGCTCAATGGGGACTTCCTGGTACGGGAGAGCACGACCACACCTGGCCAGTATGTGCT CACTGGCTTGCAGAGTGGGCAGCCTAAGCATTTGCTACTGGTGGACCCTGAGGGTGTGGTTCGGACTAAGGATCACCGCTTTGAAAGTGTCAGTCACCTT ATCAGCTACCACATGGACAATCACTTGCCCATCATCTCTGCGGGCAGCGAACTGTGTCTACAGCAACCTGTGGAGCGGAAACTGTGATCTGCCCTAGCGC TCTCTTCCAGAAGATGCCCTCCAATCCTTTCCACCCTATTCCCTAACTCTCGGGACCTCGTTTGGGAGTGTTCTGTGGGCTTGGCCTTGTGTCAGAGCTG GGAGTAGCATGGACTCTGGGTTTCATATCCAGCTGAGTGAGAGGGTTTGAGTCAAAAGCCTGGGTGAGAATCCTGCCTCTCCCCAAACATTAATCACCAA AGTATTAATGTACAGAGTGGCCCCTCACCTGGGCCTTTCCTGTGCCAACCTGATGCCCCTTCCCCAAGAAGGTGAGTGCTTGTCATGGAAAATGTCCTGT GGTGACAGGCCCAGTGGAACAGTCACCCTTCTGGGCAAGGGGGAACAAATCACACCTCTGGGCTTCAGGGTATCCCAGACCCCTCTCAACACCCGCCCCC CCCATGTTTAAACTTTGTGCCTTTGACCATCTCTTAGGTCTAATGATATTTTATGCAAACAGTTCTTGGACCCCTGAATTCAATGACAGGGATGCCAACA CCTTCTTGGCTTCTGGGACCTGTGTTCTTGCTGAGCACCCTCTCCGGTTTGGGTTGGGATAACAGAGGCAGGAGTGGCAGCTGTCCCCTCTCCCTGGGGA TATGCAACCCTTAGAGATTGCCCCAGAGCCCCACTCCCGGCCAGGCGGGAGATGGACCCCTCCCTTGCTCAGTGCCTCCTGGCCGGGGCCCCTCACCCCA AGGGGTCTGTATATACATTTCATAAGGCCTGCCCTCCCATGTTGCATGCCTATGTACTCTACGCCAAAGTGCAGCCCTTCCTCCTGAAGCCTCTGCCCTG CCTCCCTTTCTGGGAGGGCGGGGTGGGGGTGACTGAATTTGGGCCTCTTGTACAGTTAACTCTCCCAGGTGGATTTTGTGGAGGTGAGAAAAGGGGCATT GAGACTATAAAGCAGTAGACAATCCCCACATACCATCTGTAGAGTTGGAACTGCATTCTTTTAAAGTTTTATATGCATATATTTTAGGGCTGTAGACTTA CTTTCCTATTTTCTTTTCCATTGCTTATTCTTGAGCACAAAATGATAATCAATTATTACATTTATACATCACCTTTTTGACTTTTCCAAGCCCTTTTACA GCTCTTGGCATTTTCCTCGCCTAGGCCTGTGAGGTAACTGGGATCGCACCTTTTATACCAGAGACCTGAGGCAGATGAAATTTATTTCCATCTAGGACTA GAAAAACTTGGGTCTCTTACCGCGAGACTGAGAGGCAGAAGTCAGCCCGAATGCCTGTCAGTTTCATGGAGGGGAAACGCAAAACCTGCAGTTCCTGAGT ACCTTCTACAGGCCCGGCCCAGCCTAGGCCCGGGGTGGCCACACCACAGCAAGCCGGCCCCCCCTCTTTTGGCCTTGTGGATAAGGGAGAGTTGACCGTT TTCATCCTGGCCTCCTTTTGCTGTTTGGATGTTTCCACGGGTCTCACTTATACCAAAGGGAAAACTCTTCATTAAAGTCCGTATTTCTTCTACCTCTGCC TCTTAACCCTTCTGGGAAGCCTATTGTGTACGCCGCGGGCCCTCAGCGGCAGACGGGGAGGCGGGAGGTCGCGGCC

SHC1.F7 (forward primer)
SHC1.R7 (reverse primer)
Single exon skipping

Frame 0

SSQPEKRLHVGRGWGLKLSGSELGSGSHLSLSL PRTSVTPGPQRSNQAKGLGIPPAWPPCPNWQGGQ AGQQPLFHLNYGSPAPQAQVQSTPE*VSVIAGG RGFWVHPPGGAAFPISFIPGAHPASSAWGR*SH YPVLLLPPDEQPEAGQPGWGAPRV*GGARKGS** WGGDRRGSHARLRPPTPPPGHEQAEWRRRAQDS GGRGPAWGRGVDPPRELCQ*AHAGLAASQRQSH GTRGFLLGSVHGLCGGPPVNACPGLQHPDSGHQG GHQSGV*GCAGC*GGDKEEKAL*PPAQLYPGEE *PEICWNANHSHRLHQQPQPHGRRLQTDHRQPP HAIYLICIRRGSGHSRVCRLCCQRPCESESLPHS GVSRRACPGCHQHHWPGLRVALQTIPQEPTQTG HPS*QDGWL*WLSMG*GGGRAT*PSVL**LPGE GTPLGGGGRHEASGRSRSRGCSTHCTQCPDPQPL GSYIACRTACWGRSRSPQTDATSTTLSSRQRAF **SLLCQRPEPRQGPASSGWCWAPQSCYQWQCT PGPV*HEALRRCSSRASTSPVGVHG*AAPRGALV PWEAEPAGG*GTAAAQWGLPGTGEHDHTWPVCA HWLAEWAA*AFATGGP*GCGSD*GSPL*KCQSP YQLPHGQSLAHHLCGQRTVSTATCGAETVICPSA LFQKMPSNPFHPIP*LSGPRLGVFCGLGLVSEL GVAWTLGFISS*VRGFESKAWVRILPLPKH*SP KY*CTEWPLTWAFPVPT*CPFPKKVSACHGKCPV VTGPVEQSPFWARGNKSHLWASGYPRPLSTPAP PMFKLCAFDHLLGLMIFYANSSWTPEFNDRDAN TFLASGTCVLAEHPLRFGLG*QRQEWQLSPLPGD MQPLEIAPEPHSRPGGRWTPPLLSASWPGPLTP RGLYIHFIRPALPCCMPMYSTPKCSPSS*SLCP ASLSGRAGWG*LNLGLLYS*LSQVDFVEVRKGAL RL*SSRQSPHTICRVGTAFF*SFICIYFRAVDL LSYFLFHCLFLSTK**SIITFIHHLFDFSKPFY SSWHFPRLGL*GNWDRTFYTRDLRQMKFISI*D* KNLGLLPRD*EAEVSPNACQFHGGETQNLQFLS TFYRPGPA*ARGGHTTASRPPLFWPCG*GRVDR FHPGLLLLFGCFHGSHLYQRENSSLKSVFLLPLP LNPSGKPIVYAAGPQRQTGRREVAA

Frame 1

LLSQRRGSTLGGDGA*NCLGLSWGAEATCPSPS PGLL*LLGHRGPTRLRAWGYPLPGPLAQTGRGA RLGSSPSFTSTMDLLPPKPKYNPLRNESLSSLEE GASGSTPPEELPSPSASSLGPILPPLPGDDSPT TLCSFFPRMSNLRLANPAGGRPGSKGEPGRAAD DGEGIVGAAMPDSGPLPLLQDMNKLSGGGGRRTR VEGGQLGGEEWTRHGSFVNKPTRGWLHPNDKVM GPGVSYLVRYMGCVEVLQSMRALDFNTRTQVTR EAISLVCEAVPGAKGATRRRKPCSRPLSSILGRS NLKFAGMPITLTVSTSSLNLMAADCKQIIANHH MQSISFASGGDPDTAEYVAYVAKDPVNQRACHI LECPEGLAQDVISTIGQAFELRFKQYLRNPPKLV TPHDRMAGFDGSAWDEEEEEPPDHQYYNDFPGK EPPLGGVVDMRLREGAAPGAARPTAPNAQTPSH LGATLPVGQPVGGDPEVRKQMPPPPPCPAGRELF DDPSYVNVQNLDKARQAVGGAGPPNPAINGSAP RDLFDMKPFEDALRVPPPPQSVSMAEQLRGEPW FHGKLSRREAEALLQLNGDFLVRESTTTPGQYVL TGLQSGQPKHLLLVDPEGVVRTKDHRFESVSHL ISYHMDNHLPIISAGSELCLQQPVERKL*SALA LSSRRCPPILSTLFPNSRDLVWECSVGLALCQSW E*HGLWVSYPAE*EGLSQKPG*ESCLSPNINHQ SINVQSGPSPGPFLCQPDAPSPRR*VLVMENVL W*QAQWNSHPSGQGGTNHTSGLQGIPDPSQHPPP PCLNFVPLTIS*V**YFMQTVLGPLNSMTGMPT PSWLLGPVFLLSTLSGLGWDNRGRSGSCPLSLG ICNP*RLPQSPTPGQAGDGPLPCSVPPGRGPSPQ GVCIYIS*GLPSHVACLCTLRQSAALPPEASAL PPFLGGRGGGD*IWASCTVNSPRWILWR*EKGH *DYKAVDNPHIPSVELELHSFKVLYAYILGL*TY FPIFFSIAYS*AQNDNQLLHLYITFLTFPSPFT ALGIFLA*ACEVTGIAPFIPET*GR*NLFPSRT RKTWVSYRETERQKSARMPVSFMEGKRKTCSS*V PSTGPAQPRPGVATPQQAGPPSFGLVDKGELTV FILASFCCLDVSTGLTYTKGKTLH*SPYFFYLC LLTLLGSLLCTPRALSGRRGGGRSR

Frame 2

FSAREEAPRWAGMGPETVWV*AGERKPLVPLPP QDFCDSWATEVQPG*GPGDTPCLAPLPKLAGGP GWAAAPLSPQLWISCPPSPSTIHSGMSLCHRWR KGLLGPPPRRSCLPHQLHPWGPSCLLCLGTIVPL PCAPSSPG*AT*GWPTRLGGAQGLRGSQEGQLM MGRGS*GQPCQTQAPYPSSRT*TS*VEAAGAGL GWKGASLGARSGPATGALSISPRGAGCIPTTKSW DPGFPTWFGTWVVWRSSSQCVPWTSTPGLRSPG RPSVWCVRLCRVLRGRQGGESPVAARSALSWGG VT*NLLECQSLSPSPPAASTSWPQTANRSSPTTT CNLSHLHPAGIRTQPSMSPMLPKTL*IREPATF WSVPKGLPRMSSAPLARPSSCASNNTSGTHPNW SPLMTGWLALMAQHGMRRRKSHLTISTIMTSRGR NPPWGGW*T*GFGKEPLQGLLDPLHPMPRPPAT WELHCL*DSLLGEIQKSANRCHLHHPVQQAESF LMIPPMSTSRT*TRPGKQWVVLGPPILLSMAVHP GTCLT*SPSKMLFACLHLPSRCPWLSSSEGSPG SMGS*AGGRLRHCCSSMGTSWYGRARPHLASMC SLACRVGSLSICYWWTLRVWFGLRITALKVSVTL SATTWTITCPSSLRAANCVYSNLWSGNCDLP*R SLPEDALQSFPPYSLTLGTSFGSVLWAWPCVRA GSSMDSGFHIQLSERV*VKSLGENPASPQTLITK VLMYRVAPHLGLSCANLMPLPQEGECLSWKMSC GDRPSGTVTLLGKGEQITPLGFRVSQTPLNTRP PHV*TLCL*PSLRSNDILCKQFLDP*IQ*QGCQH LLGFWDLCSC*APSPVWVGITEAGVAAVPSPWG YATLRDCPRAPLPARREMDPSLAQCLLAGAPHP KGSVYTFHKACPPMLHAYVLYAKVQPFLLKPLPC LPFWEGGVGVTEFGPLVQLTLPGGFCGGEKRGI ETIKQ*TIPTYHL*SWNCILLKFYMHIF*GCRL TFLFSFPLLILEHKMIINYYIYTSPF*LFQALLQ LLAFSSPRPVR*LGSHLLYQRPEADEIYFHLGL EKLGSLTARLRGRSQPECLSVSWRGNAKPAVPE YLLQARPSLGPGWPHHSKPAPPLLALWIRES*PF SSWPPFAVWMFPRVSLIPKGKLFIKVRISSTSA S*PFWEAYCVRRGPSAADGEAGGRG