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Sequence BCAS1.dAug05
GGTGGCAGCTGGACCGGGGCAGGACACAGATAAAACCCCAGGGCACGCCCCGGCCCAAGACAAGGTCCTCTCTGCCGCCAGGGATCCCACGCTTCTCCCACCTGAGACAGGGGGAGCAGGAGGAGAAGCTCCCTCCAAGCCCAAGGACTCCAGCTTTTTTGACAAATTCTTCAAGCTGGACAAGGGACAGGAAAAGGTGC
CAGGTGACAGCCAACAGGAAGCCAAGAGGGCAGAGCATCAAGACAAGGTGGATGAGGTTCCTGGCTTATCAGGGCAGTCCGATGATGTCCCTGCAGGGAA
GGACATAGTTGACGGCAAGGAAAAAGAAGGACAAGAACTTGGAACTGCGGATTGCTCTGTCCCTGGGGACCCAGAAGGACTGGAGACTGCAAAGGACGAT
TCCCAGGCAGCAGCTATAGCAGAGAATAATAATTCCATCATGAGTTTCTTTAAAACTCTGGTTTCACCTAACAAAGCTGAAACAAAAAAGGACCCAGAAG
ACACGGCATCGAAAGCAGAAAGTGTCTGTGATGGACAAGCTGGTCAGAAGACATCCGAGATCCAGGCTAGAGGCACCAAGAAAAAGCACCTGGATAGCCC
CAGGCTAGGACTCGCCTTTAGAAAATTCTTTAGGCATAAGGGTGCTGAAAAGTCACCCACCACTTCAGCTGACCTTAAGTCAGACAAAGCCAACTTTACA
TCCCAGGAGACCCAAGGGGCTGGCAAGAATTCCAAAGGATGCAACCCATCGGGGCACACACAGTCCGTGACAACCCCTGAACCTGCGAAGGAAGGCACCA
AGGAGAAATCAGGACCCACCTCTCTGCCTCTGGGCAAACTGTTTTGGAAAAAGACATCAGACTCCACAGAAAAGACTATCACACCGCCAGAGCCTGAACC
AACAGGAGCACC
- BCAS1.F1 (forward primer)
- BCAS1.R1 (reverse primer)
- Single exon skipping
Frame 0
GGSWTGAGHR*NPRARPGPRQGPLCRQGSHASPT*DRGSRRRSSLQAQGLQLF*QILQAGQGTGKGA
R*QPTGSQEGRASRQGG*GSWLIRAVR*CPCRE
GHS*RQGKRRTRTWNCGLLCPWGPRRTGDCKGR
FPGSSYSRE**FHHEFL*NSGFT*QS*NKKGPRR
HGIESRKCL*WTSWSEDIRDPG*RHQEKAPG*P
QARTRL*KIL*A*GC*KVTHHFS*P*VRQSQLY
IPGDPRGWQEFQRMQPIGAHTVRDNP*TCEGRHQ
GEIRTHLSASGQTVLEKDIRLHRKDYHTARA*T
NRST
Frame 1
VAAGPGQDTDKTPGHAPAQDKVLSAARDPTLLPPETGGAGGEAPSKPKDSSFFDKFFKLDKGQEKV
PGDSQQEAKRAEHQDKVDEVPGLSGQSDDVPAGK
DIVDGKEKEGQELGTADCSVPGDPEGLETAKDD
SQAAAIAENNNSIMSFFKTLVSPNKAETKKDPE
DTASKAESVCDGQAGQKTSEIQARGTKKKHLDSP
RLGLAFRKFFRHKGAEKSPTTSADLKSDKANFT
SQETQGAGKNSKGCNPSGHTQSVTTPEPAKEGT
KEKSGPTSLPLGKLFWKKTSDSTEKTITPPEPEP
TGA
Frame 2
WQLDRGRTQIKPQGTPRPKTRSSLPPGIPRFSHLRQGEQEEKLPPSPRTPAFLTNSSSWTRDRKRC
QVTANRKPRGQSIKTRWMRFLAYQGSPMMSLQG
RT*LTARKKKDKNLELRIALSLGTQKDWRLQRTI
PRQQL*QRIIIPS*VSLKLWFHLTKLKQKRTQK
TRHRKQKVSVMDKLVRRHPRSRLEAPRKSTWIA
PG*DSPLENSLGIRVLKSHPPLQLTLSQTKPTLH
PRRPKGLARIPKDATHRGTHSP*QPLNLRRKAP
RRNQDPPLCLWANCFGKRHQTPQKRLSHRQSLN
QQEH
Sequence BCAS1.bAug05
GTATTCAGCCAGCAGGCTCTGCACAAGGAAGCAAAGTGCAGGGAGCCAGGCTCCACCGACAGCCAGGCACTGGGCAGCACGCACTGGAGACCCAGGACCCTGTGCAGGAGCAGCTCCGGGTGACACGAGGGGACTGAAGATACTCCCACAGGGGCTCAGCAGGAGCAATGGGTAACCAAATGAGTGTTCCCCAAAGAGTT
GAAGACCAAGAGAATGAACCAGAAGCAGAGACTTACCAGGACAACGCGTCTGCTCTGAACGGGGTTCCAGTGGTGGTGTCGACCCACACAGTTCAGCACT
TAGAGGAAGTCGACTTGGGAATAAGTGTCAAGACGGATAATGTGGCCACTTCTTCCCCCGAGACAACGGAGATAAGTGCTGTTGCGGATGCCAACGGAAA
GAATCTTGGGAAAGAGGCCAAACCCGAGGCACCAGCTGCTAAATCTCGTTTTTTCTTGATGCTCTCTCGGCCTGTACCAGGACGTACCGGAGACCAAGCC
GCAGATTCATCCCTTGGATCAGTGAAGCTTGATGTCAGCTCCAATAAAGCTCCAGCGAACAAAGACCCAAGTGAGAGCTGGACACTTCCGGTGGCAGCTG
GACCGGGGCAGGACACAGATAAAACCCCAGGGCACGCCCCGGCCCAAGACAAGGTCCTCTCTGCCGCCAGGGATCCCACGCTTCTCCCACCTGAGACAGG
GGGAGCAGGAGGAGAAGCTCCCTCCAAGCCCAAGGACTCCAGCTTTTTTGACAAATTCTTCAAGCTGGACAAGGGACAGGAAAAGGTGCCAGGTGACAGC
CAACAGGAAGCCAAGAGGGCAGAGCATCAAGACAAGGTGGATGAGGTTCCTGGCTTATCAGGGCAGTCCGATGATGTCCCTGCAGGGAAGGACATAGTTG
ACGGCAAGGAAAAAGAAGGACAAGAACTTGGAACTGCGGATTGCTCTGTCCCTGGGGACCCAGAAGGACTGGAGACTGCAAAGGACGATTCCCAGGCAGC
AGCTATAGCAGAGAATAATAATTCCATCATGAGTTTCTTTAAAACTCTGGTTTCACCTAACAAAGCTGAAACAAAAAAGGACCCAGAAGACACGGGTGCT
GAAAAGTCACCCACCACTTCAGCTGACCTTAAGTCAGACAAAGCCAACTTTACATCCCAGGAGACCCAAGGGGCTGGCAAGAATTCCAAAGGATGCAACC
CATCGGGGCACACACAGTCCGTGACAACCCCTGAACCTGCGAAGGAAGGCACCAAGGAGAAATCAGGACCCACCTCTCTGCCTCTGGGCAAACTGTTTTG
GAAAAAGTCAGTTAAAGAGGACTCAGTCCCCACAGGTGCGGAGGAGAATACATCAGACTCCACAGAAAAGACTATCACACCGCCAGAGCCTGAACCAACA
GGAGCACCACAGAAGGGTAAAGAGGGCTCCTCGAAGGACAAGAAGTCAGCAGCCGAGATGAACAAGCAGAAGAGCAACAAGCAGGAAGCCAAAGAACCAG
CCCAGTGCACAGAGCAGGCCACGGTGGACACGAACTCACTGCAGAATGGGGACAAGCTCCAAAAGAGACCTGAGAAGCGGCAGCAGTCCCTTGGGGGCTT
CTTTAAAGGCCTGGGACCAAAGCGGATGTTGGATGCTCAAGTGCAAACAGACCCAGTATCCATCGGACCAGTTGGCAAATCCAAGTAAACAAATCAGCAC
GGTTCCCACCAGGTTCTCCTGCCACCAAGATGTGTTCTCCTTACTCCATCTCCTCCCCAAACACGCTCCATGTATATATTCTTCTGATGGCCAGCAAATG
AAATTCTGCCTAGAAATTAAGCCCGAGCTGTTGTATATTGAGGTGTATTATTTACGTCTCTGGTCCAGTCTTTTCTGGCAAATAACAGTAAAGATGGTTT
AGCAGGTCACCTAGTTGGGTCAGAAGAGTCGATGATCACCAAGCAGGAAAGGGAGGGAATAGAGGAATGTGTTCGGGTTAAGTGATGAAAATGGCAGTGG
TGGCCGGGCGTGGTGGCTCTCGCCTGTAATCTCAGCACTTTGGGAGGCCGAGGCAGGTGGATCACCTGAGGTCAGGAGTTCAAGACTAGCCTGGCCAACA
TCATGAAACCCCGTCTCTACTAAAAATACAAAAATTAGCCAGGCATGGTGGCACACACCTGTAGTCCCAGCTACTCGGGAGCCCAACGCACGAGAACCGC
TTGTACCCAGGAGGTGGAGGTTGCAGTGAGCCGAAGTTGCACCATTGCACTCCACCCTGGGCGACAGAGCAAGATTCTATCAAAAAAAAAAAAAGGCAGT
GGCAAGTAAGTTATAGAAGAGAAATGCTGCTAGAAGGAATTAAGCGTTGTAGTAAATGCGTGCTTATCCTCTAAGCTTGAAGAAGGGAGACGAAAATCCA
TTTGTTTAAATTCACATCTCAAGGAGGGAGAACCCGGGCTGTGTTGGGTGGTTGCCAATTTCCTAGAACGGAATGTGTGGGGTATAGAAAAAGGAATGAA
TAAGCGTTGTTTTTCAAATAGGGTCCTTGTAAGTTATTGATGAGAGGGAAAAGATTGACTGGGGAGGGCTTAAAATGATTTGGGAAAACAATTGCTTTTG
AGGCTCAGTGACAACGGCAAAGATTACAACTTAAAAAAAAAAAATAAATAAAAAATAAAGGAAGTTGCACGGTTATTTTGCAACACAAGGGGGCGGCAAG
GTCCCCATTTTTATCCTGTAATACTGTATCCCTAACAAAGATTTGGTCTCTGCTATCTTACATTATTAATGTTTCTCAGATGGCTGAGGGGCTCGCTTCA
TCTGTTCCGTCTGACACTTATCTCAAGTGTGTCTGTCATTCCTAATGTTCTCAGGATGTGCTCTGATAAAACCCTCCCCATAACCTCAGTTAATAAAAAT
TTACAGAAGACTTCTCAAATACCTGAGTTGTTTTTAATACCTGTACAAAGGAGTAAATAGGACCCTGAGTCTATTAAAATGTAATTCAAAGTAGCATATG
ATTGACTGACAGTCATGTAAACTGTATCTTTCTTTTTCTGATTTAATAAAAAATACATTTACTTCTAAAGTATCTTTTAATTTTCAACTTCAAAGCAGTC
CCACTCTATATCTGAGATGACTCTAACTTCTACATTATAAAATCCATACTCTTTCTCCATTATCTTCTTTGGAAGATCATTCTCTCCCAGATCTTCCCGG
ATTTCCTTGTATATGGGCACACACACACACAAATGTGTGCAGTTGTATGTTTGCATGTACACACACATGCATGTATAGAATAGTGCTGTCTTAGGCCAGG
TTGCTTGGAGCAGAGCCTGAGATGGGGATTTGGACGCATAGGATATACTGAGAGTGGGCTCCGAGGGAAAGGGAGTAAGAGAAAAAGAAGAGGGCAAGAA
AAGAGTTGGGCAAGGGTTGGTTTCAACTGGAGCATGAATTGCACCACCCAGATAGTTTGACCTTGAGTCAAGGGAGCCAGCCTTTGAACCCTGGTAGGTG
AATTCTGGGCTGCCCTGGGAGGTGGGGGG
- BCAS1.F1 (forward primer)
- BCAS1.R1 (reverse primer)
- Single exon skipping
Frame 0
VFSQQALHKEAKCREPGSTDSQALGSTHWRPRTLCRSSSG*HEGTEDTPTGAQQEQWVTK*VFPKEL
KTKRMNQKQRLTRTTRLL*TGFQWWCRPTQFST
*RKSTWE*VSRRIMWPLLPPRQRR*VLLRMPTE
RILGKRPNPRHQLLNLVFS*CSLGLYQDVPETKP
QIHPLDQ*SLMSAPIKLQRTKTQVRAGHFRWQL
DRGRTQIKPQGTPRPKTRSSLPPGIPRFSHLRQ
GEQEEKLPPSPRTPAFLTNSSSWTRDRKRCQVTA
NRKPRGQSIKTRWMRFLAYQGSPMMSLQGRT*L
TARKKKDKNLELRIALSLGTQKDWRLQRTIPRQ
QL*QRIIIPS*VSLKLWFHLTKLKQKRTQKTRVL
KSHPPLQLTLSQTKPTLHPRRPKGLARIPKDAT
HRGTHSP*QPLNLRRKAPRRNQDPPLCLWANCF
GKSQLKRTQSPQVRRRIHQTPQKRLSHRQSLNQQ
EHHRRVKRAPRRTRSQQPR*TSRRATSRKPKNQ
PSAQSRPRWTRTHCRMGTSSKRDLRSGSSPLGA
SLKAWDQSGCWMLKCKQTQYPSDQLANPSKQIST
VPTRFSCHQDVFSLLHLLPKHAPCIYSSDGQQM
KFCLEIKPELLYIEVYYLRLWSSLFWQITVKMV
*QVT*LGQKSR*SPSRKGRE*RNVFGLSDENGSG
GRAWWLSPVISALWEAEAGGSPEVRSSRLAWPT
S*NPVSTKNTKISQAWWHTPVVPATREPNAREP
LVPRRWRLQ*AEVAPLHSTLGDRARFYQKKKKAV
ASKL*KRNAARRN*AL**MRAYPLSLKKGDENP
FV*IHISRRENPGCVGWLPIS*NGMCGV*KKE*
ISVVFQIGSL*VIDEREKIDWGGLKMIWENNCF*
GSVTTAKITT*KKKINKK*RKLHGYFATQGGGK
VPIFIL*YCIPNKDLVSAILHY*CFSDG*GARF
ICSV*HLSQVCLSFLMFSGCALIKPSP*PQLIKI
YRRLLKYLSCF*YLYKGVNRTLSLLKCNSK*HM
ID*QSCKLYLSFSDLIKNTFTSKVSFNFQLQSS
PTLYLR*L*LLHYKIHTLSPLSSLEDHSLPDLPG
FPCIWAHTHTNVCSCMFACTHTCMYRIVLS*AR
LLGAEPEMGIWTHRIY*EWAPRERE*EKKKRAR
KELGKGWFQLEHELHHPDSLTLSQGSQPLNPGR*
ILGCPGRWG
Frame 1
YSASRLCTRKQSAGSQAPPTARHWAARTGDPGPCAGAAPGDTRGLKILPQGLSRSNG*PNECSPKS
*RPRE*TRSRDLPGQRVCSERGSSGGVDPHSSAL
RGSRLGNKCQDG*CGHFFPRDNGDKCCCGCQRK
ESWERGQTRGTSC*ISFFLDALSACTRTYRRPS
RRFIPWISEA*CQLQ*SSSEQRPK*ELDTSGGSW
TGAGHR*NPRARPGPRQGPLCRQGSHASPT*DR
GSRRRSSLQAQGLQLF*QILQAGQGTGKGAR*Q
PTGSQEGRASRQGG*GSWLIRAVR*CPCREGHS*
RQGKRRTRTWNCGLLCPWGPRRTGDCKGRFPGS
SYSRE**FHHEFL*NSGFT*QS*NKKGPRRHGC
*KVTHHFS*P*VRQSQLYIPGDPRGWQEFQRMQP
IGAHTVRDNP*TCEGRHQGEIRTHLSASGQTVL
EKVS*RGLSPHRCGGEYIRLHRKDYHTARA*TN
RSTTEG*RGLLEGQEVSSRDEQAEEQQAGSQRTS
PVHRAGHGGHELTAEWGQAPKET*EAAAVPWGL
L*RPGTKADVGCSSANRPSIHRTSWQIQVNKSA
RFPPGSPATKMCSPYSISSPNTLHVYILLMASK*
NSA*KLSPSCCILRCIIYVSGPVFSGK*Q*RWF
SRSPSWVRRVDDHQAGKGGNRGMCSG*VMKMAV
VAGRGGSRL*SQHFGRPRQVDHLRSGVQD*PGQH
HETPSLLKIQKLARHGGTHL*SQLLGSPTHENR
LYPGGGGCSEPKLHHCTPPWATEQDSIKKKKRQ
WQVSYRREMLLEGIKRCSKCVLIL*A*RRETKIH
LFKFTSQGGRTRAVLGGCQFPRTECVGYRKRNE
*ALFFK*GPCKLLMRGKRLTGEGLK*FGKTIAF
EAQ*QRQRLQLKKKK*IKNKGSCTVILQHKGAAR
SPFLSCNTVSLTKIWSLLSYIINVSQMAEGLAS
SVPSDTYLKCVCHS*CSQDVL**NPPHNLS**K
FTEDFSNT*VVFNTCTKE*IGP*VY*NVIQSSI*
LTDSHVNCIFLFLI**KIHLLLKYLLIFNFKAV
PLYI*DDSNFYIIKSILFLHYLLWKIILSQIFP
DFLVYGHTHTQMCAVVCLHVHTHACIE*CCLRPG
CLEQSLRWGFGRIGYTESGLRGKGSKRKRRGQE
KSWARVGFNWSMNCTTQIV*P*VKGASL*TLVG
EFWAALGGGG
Frame 2
IQPAGSAQGSKVQGARLHRQPGTGQHALETQDPVQEQLRVTRGD*RYSHRGSAGAMGNQMSVPQRV
EDQENEPEAETYQDNASALNGVPVVVSTHTVQH
LEEVDLGISVKTDNVATSSPETTEISAVADANGK
NLGKEAKPEAPAAKSRFFLMLSRPVPGRTGDQA
ADSSLGSVKLDVSSNKAPANKDPSESWTLPVAA
GPGQDTDKTPGHAPAQDKVLSAARDPTLLPPETG
GAGGEAPSKPKDSSFFDKFFKLDKGQEKVPGDS
QQEAKRAEHQDKVDEVPGLSGQSDDVPAGKDIV
DGKEKEGQELGTADCSVPGDPEGLETAKDDSQAA
AIAENNNSIMSFFKTLVSPNKAETKKDPEDTGA
EKSPTTSADLKSDKANFTSQETQGAGKNSKGCN
PSGHTQSVTTPEPAKEGTKEKSGPTSLPLGKLFW
KKSVKEDSVPTGAEENTSDSTEKTITPPEPEPT
GAPQKGKEGSSKDKKSAAEMNKQKSNKQEAKEP
AQCTEQATVDTNSLQNGDKLQKRPEKRQQSLGGF
FKGLGPKRMLDAQVQTDPVSIGPVGKSK*TNQH
GSHQVLLPPRCVLLTPSPPQTRSMYIFF*WPAN
EILPRN*ARAVVY*GVLFTSLVQSFLANNSKDGL
AGHLVGSEESMITKQEREGIEECVRVK**KWQW
WPGVVALACNLSTLGGRGRWIT*GQEFKTSLAN
IMKPRLY*KYKN*PGMVAHTCSPSYSGAQRTRTA
CTQEVEVAVSRSCTIALHPGRQSKILSKKKKGS
GK*VIEEKCC*KELSVVVNACLSSKLEEGRRKS
ICLNSHLKEGEPGLCWVVANFLERNVWGIEKGMN
KRCFSNRVLVSY**EGKD*LGRA*NDLGKQLLL
RLSDNGKDYNLKKKNK*KIKEVARLFCNTRGRQ
GPHFYPVILYP*QRFGLCYLTLLMFLRWLRGSLH
LFRLTLISSVSVIPNVLRMCSDKTLPITSVNKN
LQKTSQIPELFLIPVQRSK*DPESIKM*FKVAY
D*LTVM*TVSFFF*FNKKYIYF*SIF*FSTSKQS
HSISEMTLTSTL*NPYSFSIIFFGRSFSPRSSR
ISLYMGTHTHKCVQLYVCMYTHMHV*NSAVLGQ
VAWSRA*DGDLDA*DILRVGSEGKGVREKEEGKK
RVGQGLVSTGA*IAPPR*FDLESREPAFEPW*V
NSGLPWEVG
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