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Sequence BCAS1.dAug05
GGTGGCAGCTGGACCGGGGCAGGACACAGATAAAACCCCAGGGCACGCCCCGGCCCAAGACAAGGTCCTCTCTGCCGCCAGGGATCCCACGCTTCTCCCACCTGAGACAGGGGGAGCAGGAGGAGAAGCTCCCTCCAAGCCCAAGGACTCCAGCTTTTTTGACAAATTCTTCAAGCTGGACAAGGGACAGGAAAAGGTGC
CAGGTGACAGCCAACAGGAAGCCAAGAGGGCAGAGCATCAAGACAAGGTGGATGAGGTTCCTGGCTTATCAGGGCAGTCCGATGATGTCCCTGCAGGGAA
GGACATAGTTGACGGCAAGGAAAAAGAAGGACAAGAACTTGGAACTGCGGATTGCTCTGTCCCTGGGGACCCAGAAGGACTGGAGACTGCAAAGGACGAT
TCCCAGGCAGCAGCTATAGCAGAGAATAATAATTCCATCATGAGTTTCTTTAAAACTCTGGTTTCACCTAACAAAGCTGAAACAAAAAAGGACCCAGAAG
ACACGGCATCGAAAGCAGAAAGTGTCTGTGATGGACAAGCTGGTCAGAAGACATCCGAGATCCAGGCTAGAGGCACCAAGAAAAAGCACCTGGATAGCCC
CAGGCTAGGACTCGCCTTTAGAAAATTCTTTAGGCATAAGGGTGCTGAAAAGTCACCCACCACTTCAGCTGACCTTAAGTCAGACAAAGCCAACTTTACA
TCCCAGGAGACCCAAGGGGCTGGCAAGAATTCCAAAGGATGCAACCCATCGGGGCACACACAGTCCGTGACAACCCCTGAACCTGCGAAGGAAGGCACCA
AGGAGAAATCAGGACCCACCTCTCTGCCTCTGGGCAAACTGTTTTGGAAAAAGACATCAGACTCCACAGAAAAGACTATCACACCGCCAGAGCCTGAACC
AACAGGAGCACC
- BCAS1.F2 (forward primer)
- BCAS1.R1 (reverse primer)
- Multiple exon skipping
Frame 0
GGSWTGAGHR*NPRARPGPRQGPLCRQGSHASPT*DRGSRRRSSLQAQGLQLF*QILQAGQGTGKGA
R*QPTGSQEGRASRQGG*GSWLIRAVR*CPCRE
GHS*RQGKRRTRTWNCGLLCPWGPRRTGDCKGR
FPGSSYSRE**FHHEFL*NSGFT*QS*NKKGPRR
HGIESRKCL*WTSWSEDIRDPG*RHQEKAPG*P
QARTRL*KIL*A*GC*KVTHHFS*P*VRQSQLY
IPGDPRGWQEFQRMQPIGAHTVRDNP*TCEGRHQ
GEIRTHLSASGQTVLEKDIRLHRKDYHTARA*T
NRST
Frame 1
VAAGPGQDTDKTPGHAPAQDKVLSAARDPTLLPPETGGAGGEAPSKPKDSSFFDKFFKLDKGQEKV
PGDSQQEAKRAEHQDKVDEVPGLSGQSDDVPAGK
DIVDGKEKEGQELGTADCSVPGDPEGLETAKDD
SQAAAIAENNNSIMSFFKTLVSPNKAETKKDPE
DTASKAESVCDGQAGQKTSEIQARGTKKKHLDSP
RLGLAFRKFFRHKGAEKSPTTSADLKSDKANFT
SQETQGAGKNSKGCNPSGHTQSVTTPEPAKEGT
KEKSGPTSLPLGKLFWKKTSDSTEKTITPPEPEP
TGA
Frame 2
WQLDRGRTQIKPQGTPRPKTRSSLPPGIPRFSHLRQGEQEEKLPPSPRTPAFLTNSSSWTRDRKRC
QVTANRKPRGQSIKTRWMRFLAYQGSPMMSLQG
RT*LTARKKKDKNLELRIALSLGTQKDWRLQRTI
PRQQL*QRIIIPS*VSLKLWFHLTKLKQKRTQK
TRHRKQKVSVMDKLVRRHPRSRLEAPRKSTWIA
PG*DSPLENSLGIRVLKSHPPLQLTLSQTKPTLH
PRRPKGLARIPKDATHRGTHSP*QPLNLRRKAP
RRNQDPPLCLWANCFGKRHQTPQKRLSHRQSLN
QQEH
Sequence BCAS1.aAug05
GTATTCAGCCAGCAGGCTCTGCACAAGGAAGCAAAGTGCAGGGAGCCAGGCTCCACCGACAGCCAGGCACTGGGCAGCACGCACTGGAGACCCAGGACCCTGTGCAGGAGCAGCTCCGGGTGACACGAGGGGACTGAAGATACTCCCACAGGGGCTCAGCAGGAGCAATGGGTAACCAAATGAGTGTTCCCCAAAGAGTT
GAAGACCAAGAGAATGAACCAGAAGCAGAGACTTACCAGGACAACGCGTCTGCTCTGAACGGGGTTCCAGTGGTGGTGTCGACCCACACAGTTCAGCACT
TAGAGGAAGTCGACTTGGGAATAAGTGTCAAGACGGATAATGTGGCCACTTCTTCCCCCGAGACAACGGAGATAAGTGCTGTTGCGGATGCCAACGGAAA
GAATCTTGGGAAAGAGGCCAAACCCGAGGCACCAGCTGCTAAATCTCGTTTTTTCTTGATGCTCTCTCGGCCTGTACCAGGACGTACCGGAGACCAAGCC
GCAGATTCATCCCTTGGATCAGTGAAGCTTGATGTCAGCTCCAATAAAGCTCCAGCGAACAAAGACCCAAGTGAGAGCTGGACACTTCCGGTGGCAGCTG
GACCGGGGCAGGACACAGATAAAACCCCAGGGCACGCCCCGGCCCAAGACAAGGTCCTCTCTGCCGCCAGGGATCCCACGCTTCTCCCACCTGAGACAGG
GGGAGCAGGAGGAGAAGCTCCCTCCAAGCCCAAGGACTCCAGCTTTTTTGACAAATTCTTCAAGCTGGACAAGGGACAGGAAAAGGTGCCAGGTGACAGC
CAACAGGAAGCCAAGAGGGCAGAGCATCAAGACAAGGTGGATGAGGTTCCTGGCTTATCAGGGCAGTCCGATGATGTCCCTGCAGGGAAGGACATAGTTG
ACGGCAAGGAAAAAGAAGGACAAGAACTTGGAACTGCGGATTGCTCTGTCCCTGGGGACCCAGAAGGACTGGAGACTGCAAAGGACGATTCCCAGGCAGC
AGCTATAGCAGAGAATAATAATTCCATCATGAGTTTCTTTAAAACTCTGGTTTCACCTAACAAAGCTGAAACAAAAAAGGACCCAGAAGACACGGGTGCT
GAAAAGTCACCCACCACTTCAGCTGACCTTAAGTCAGACAAAGCCAACTTTACATCCCAGGAGACCCAAGGGGCTGGCAAGAATTCCAAAGGATGCAACC
CATCGGGGCACACACAGTCCGTGACAACCCCTGAACCTGCGAAGGAAGGCACCAAGGAGAAATCAGGACCCACCTCTCTGCCTCTGGGCAAACTGTTTTG
GAAAAAGTCAGTTAAAGAGGACTCAGTCCCCACAGGTGCGGAGGAGAATGTGGTGTGTGAGTCACCAGTAGAGATTATAAAGTCCAAGGAAGTAGAATCA
GCCTTACAAACAGTGGACCTCAACGAAGGAGATGCTGCACCTGAACCCACAGAAGCGAAACTCAAAAGAGAAGAAAGCAAACCAAGAACCTCTCTGATGG
CGTTTCTCAGACAAATGTCAGTGAAAGGGGATGGAGGGATCACCCACTCAGAAGAAATAAATGGGAAAGACTCCAGCTGCCAAACATCAGACTCCACAGA
AAAGACTATCACACCGCCAGAGCCTGAACCAACAGGAGCACCACAGAAGGGTAAAGAGGGCTCCTCGAAGGACAAGAAGTCAGCAGCCGAGATGAACAAG
CAGAAGAGCAACAAGCAGGAAGCCAAAGAACCAGCCCAGTGCACAGAGCAGGCCACGGTGGACACGAACTCACTGCAGAATGGGGACAAGCTCCAAAAGA
GACCTGAGAAGCGGCAGCAGTCCCTTGGGGGCTTCTTTAAAGGCCTGGGACCAAAGCGGATGTTGGATGCTCAAGTGCAAACAGACCCAGTATCCATCGG
ACCAGTTGGCAAATCCAAGTAAACAAATCAGCACGGTTCCCACCAGGTTCTCCTGCCACCAAGATGTGTTCTCCTTACTCCATCTCCTCCCCAAACACGC
TCCATGTATATATTCTTCTGATGGCCAGCAAATGAAATTCTGCCTAGAAATTAAGCCCGAGCTGTTGTATATTGAGGTGTATTATTTACGTCTCTGGTCC
AGTCTTTTCTGGCAAATAACAGTAAAGATGGTTTAGCAGGTCACCTAGTTGGGTCAGAAGAGTCGATGATCACCAAGCAGGAAAGGGAGGGAATAGAGGA
ATGTGTTCGGGTTAAGTGATGAAAATGGCAGTGGTGGCCGGGCGTGGTGGCTCTCGCCTGTAATCTCAGCACTTTGGGAGGCCGAGGCAGGTGGATCACC
TGAGGTCAGGAGTTCAAGACTAGCCTGGCCAACATCATGAAACCCCGTCTCTACTAAAAATACAAAAATTAGCCAGGCATGGTGGCACACACCTGTAGTC
CCAGCTACTCGGGAGCCCAACGCACGAGAACCGCTTGTACCCAGGAGGTGGAGGTTGCAGTGAGCCGAAGTTGCACCATTGCACTCCACCCTGGGCGACA
GAGCAAGATTCTATCAAAAAAAAAAAAAGGCAGTGGCAAGTAAGTTATAGAAGAGAAATGCTGCTAGAAGGAATTAAGCGTTGTAGTAAATGCGTGCTTA
TCCTCTAAGCTTGAAGAAGGGAGACGAAAATCCATTTGTTTAAATTCACATCTCAAGGAGGGAGAACCCGGGCTGTGTTGGGTGGTTGCCAATTTCCTAG
AACGGAATGTGTGGGGTATAGAAAAAGGAATGAATAAGCGTTGTTTTTCAAATAGGGTCCTTGTAAGTTATTGATGAGAGGGAAAAGATTGACTGGGGAG
GGCTTAAAATGATTTGGGAAAACAATTGCTTTTGAGGCTCAGTGACAACGGCAAAGATTACAACTTAAAAAAAAAAAATAAATAAAAAATAAAGGAAGTT
GCACGGTTATTTTGCAACACAAGGGGGCGGCAAGGTCCCCATTTTTATCCTGTAATACTGTATCCCTAACAAAGATTTGGTCTCTGCTATCTTACATTAT
TAATGTTTCTCAGATGGCTGAGGGGCTCGCTTCATCTGTTCCGTCTGACACTTATCTCAAGTGTGTCTGTCATTCCTAATGTTCTCAGGATGTGCTCTGA
TAAAACCCTCCCCATAACCTCAGTTAATAAAAATTTACAGAAGACTTCTCAAATACCTGAGTTGTTTTTAATACCTGTACAAAGGAGTAAATAGGACCCT
GAGTCTATTAAAATGTAATTCAAAGTAGCATATGATTGACTGACAGTCATGTAAACTGTATCTTTCTTTTTCTGATTTAATAAAAAATACATTTACTTCT
AAAGTATCTTTTAATTTTCAACTTCAAAGCAGTCCCACTCTATATCTGAGATGACTCTAACTTCTACATTATAAAATCCATACTCTTTCTCCATTATCTT
CTTTGGAAGATCATTCTCTCCCAGATCTTCCCGGATTTCCTTGTATATGGGCACACACACACACAAATGTGTGCAGTTGTATGTTTGCATGTACACACAC
ATGCATGTATAGAATAGTGCTGTCTTAGGCCAGGTTGCTTGGAGCAGAGCCTGAGATGGGGATTTGGACGCATAGGATATACTGAGAGTGGGCTCCGAGG
GAAAGGGAGTAAGAGAAAAAGAAGAGGGCAAGAAAAGAGTTGGGCAAGGGTTGGTTTCAACTGGAGCATGAATTGCACCACCCAGATAGTTTGACCTTGA
GTCAAGGGAGCCAGCCTTTGAACCCTGGTAGGTGAATTCTGGGCTGCCCTGGGAGGTGGGGGG
- BCAS1.F2 (forward primer)
- BCAS1.R1 (reverse primer)
- Multiple exon skipping
Frame 0
VFSQQALHKEAKCREPGSTDSQALGSTHWRPRTLCRSSSG*HEGTEDTPTGAQQEQWVTK*VFPKEL
KTKRMNQKQRLTRTTRLL*TGFQWWCRPTQFST
*RKSTWE*VSRRIMWPLLPPRQRR*VLLRMPTE
RILGKRPNPRHQLLNLVFS*CSLGLYQDVPETKP
QIHPLDQ*SLMSAPIKLQRTKTQVRAGHFRWQL
DRGRTQIKPQGTPRPKTRSSLPPGIPRFSHLRQ
GEQEEKLPPSPRTPAFLTNSSSWTRDRKRCQVTA
NRKPRGQSIKTRWMRFLAYQGSPMMSLQGRT*L
TARKKKDKNLELRIALSLGTQKDWRLQRTIPRQ
QL*QRIIIPS*VSLKLWFHLTKLKQKRTQKTRVL
KSHPPLQLTLSQTKPTLHPRRPKGLARIPKDAT
HRGTHSP*QPLNLRRKAPRRNQDPPLCLWANCF
GKSQLKRTQSPQVRRRMWCVSHQ*RL*SPRK*NQ
PYKQWTSTKEMLHLNPQKRNSKEKKANQEPL*W
RFSDKCQ*KGMEGSPTQKK*MGKTPAAKHQTPQ
KRLSHRQSLNQQEHHRRVKRAPRRTRSQQPR*TS
RRATSRKPKNQPSAQSRPRWTRTHCRMGTSSKR
DLRSGSSPLGASLKAWDQSGCWMLKCKQTQYPS
DQLANPSKQISTVPTRFSCHQDVFSLLHLLPKHA
PCIYSSDGQQMKFCLEIKPELLYIEVYYLRLWS
SLFWQITVKMV*QVT*LGQKSR*SPSRKGRE*R
NVFGLSDENGSGGRAWWLSPVISALWEAEAGGSP
EVRSSRLAWPTS*NPVSTKNTKISQAWWHTPVV
PATREPNAREPLVPRRWRLQ*AEVAPLHSTLGD
RARFYQKKKKAVASKL*KRNAARRN*AL**MRAY
PLSLKKGDENPFV*IHISRRENPGCVGWLPIS*
NGMCGV*KKE*ISVVFQIGSL*VIDEREKIDWG
GLKMIWENNCF*GSVTTAKITT*KKKINKK*RKL
HGYFATQGGGKVPIFIL*YCIPNKDLVSAILHY
*CFSDG*GARFICSV*HLSQVCLSFLMFSGCAL
IKPSP*PQLIKIYRRLLKYLSCF*YLYKGVNRTL
SLLKCNSK*HMID*QSCKLYLSFSDLIKNTFTS
KVSFNFQLQSSPTLYLR*L*LLHYKIHTLSPLS
SLEDHSLPDLPGFPCIWAHTHTNVCSCMFACTHT
CMYRIVLS*ARLLGAEPEMGIWTHRIY*EWAPR
ERE*EKKKRARKELGKGWFQLEHELHHPDSLTL
SQGSQPLNPGR*ILGCPGRWG
Frame 1
YSASRLCTRKQSAGSQAPPTARHWAARTGDPGPCAGAAPGDTRGLKILPQGLSRSNG*PNECSPKS
*RPRE*TRSRDLPGQRVCSERGSSGGVDPHSSAL
RGSRLGNKCQDG*CGHFFPRDNGDKCCCGCQRK
ESWERGQTRGTSC*ISFFLDALSACTRTYRRPS
RRFIPWISEA*CQLQ*SSSEQRPK*ELDTSGGSW
TGAGHR*NPRARPGPRQGPLCRQGSHASPT*DR
GSRRRSSLQAQGLQLF*QILQAGQGTGKGAR*Q
PTGSQEGRASRQGG*GSWLIRAVR*CPCREGHS*
RQGKRRTRTWNCGLLCPWGPRRTGDCKGRFPGS
SYSRE**FHHEFL*NSGFT*QS*NKKGPRRHGC
*KVTHHFS*P*VRQSQLYIPGDPRGWQEFQRMQP
IGAHTVRDNP*TCEGRHQGEIRTHLSASGQTVL
EKVS*RGLSPHRCGGECGV*VTSRDYKVQGSRI
SLTNSGPQRRRCCT*THRSETQKRRKQTKNLSDG
VSQTNVSERGWRDHPLRRNKWERLQLPNIRLHR
KDYHTARA*TNRSTTEG*RGLLEGQEVSSRDEQ
AEEQQAGSQRTSPVHRAGHGGHELTAEWGQAPKE
T*EAAAVPWGLL*RPGTKADVGCSSANRPSIHR
TSWQIQVNKSARFPPGSPATKMCSPYSISSPNT
LHVYILLMASK*NSA*KLSPSCCILRCIIYVSGP
VFSGK*Q*RWFSRSPSWVRRVDDHQAGKGGNRG
MCSG*VMKMAVVAGRGGSRL*SQHFGRPRQVDH
LRSGVQD*PGQHHETPSLLKIQKLARHGGTHL*S
QLLGSPTHENRLYPGGGGCSEPKLHHCTPPWAT
EQDSIKKKKRQWQVSYRREMLLEGIKRCSKCVL
IL*A*RRETKIHLFKFTSQGGRTRAVLGGCQFPR
TECVGYRKRNE*ALFFK*GPCKLLMRGKRLTGE
GLK*FGKTIAFEAQ*QRQRLQLKKKK*IKNKGS
CTVILQHKGAARSPFLSCNTVSLTKIWSLLSYII
NVSQMAEGLASSVPSDTYLKCVCHS*CSQDVL*
*NPPHNLS**KFTEDFSNT*VVFNTCTKE*IGP
*VY*NVIQSSI*LTDSHVNCIFLFLI**KIHLLL
KYLLIFNFKAVPLYI*DDSNFYIIKSILFLHYL
LWKIILSQIFPDFLVYGHTHTQMCAVVCLHVHT
HACIE*CCLRPGCLEQSLRWGFGRIGYTESGLRG
KGSKRKRRGQEKSWARVGFNWSMNCTTQIV*P*
VKGASL*TLVGEFWAALGGGG
Frame 2
IQPAGSAQGSKVQGARLHRQPGTGQHALETQDPVQEQLRVTRGD*RYSHRGSAGAMGNQMSVPQRV
EDQENEPEAETYQDNASALNGVPVVVSTHTVQH
LEEVDLGISVKTDNVATSSPETTEISAVADANGK
NLGKEAKPEAPAAKSRFFLMLSRPVPGRTGDQA
ADSSLGSVKLDVSSNKAPANKDPSESWTLPVAA
GPGQDTDKTPGHAPAQDKVLSAARDPTLLPPETG
GAGGEAPSKPKDSSFFDKFFKLDKGQEKVPGDS
QQEAKRAEHQDKVDEVPGLSGQSDDVPAGKDIV
DGKEKEGQELGTADCSVPGDPEGLETAKDDSQAA
AIAENNNSIMSFFKTLVSPNKAETKKDPEDTGA
EKSPTTSADLKSDKANFTSQETQGAGKNSKGCN
PSGHTQSVTTPEPAKEGTKEKSGPTSLPLGKLFW
KKSVKEDSVPTGAEENVVCESPVEIIKSKEVES
ALQTVDLNEGDAAPEPTEAKLKREESKPRTSLM
AFLRQMSVKGDGGITHSEEINGKDSSCQTSDSTE
KTITPPEPEPTGAPQKGKEGSSKDKKSAAEMNK
QKSNKQEAKEPAQCTEQATVDTNSLQNGDKLQK
RPEKRQQSLGGFFKGLGPKRMLDAQVQTDPVSIG
PVGKSK*TNQHGSHQVLLPPRCVLLTPSPPQTR
SMYIFF*WPANEILPRN*ARAVVY*GVLFTSLV
QSFLANNSKDGLAGHLVGSEESMITKQEREGIEE
CVRVK**KWQWWPGVVALACNLSTLGGRGRWIT
*GQEFKTSLANIMKPRLY*KYKN*PGMVAHTCS
PSYSGAQRTRTACTQEVEVAVSRSCTIALHPGRQ
SKILSKKKKGSGK*VIEEKCC*KELSVVVNACL
SSKLEEGRRKSICLNSHLKEGEPGLCWVVANFL
ERNVWGIEKGMNKRCFSNRVLVSY**EGKD*LGR
A*NDLGKQLLLRLSDNGKDYNLKKKNK*KIKEV
ARLFCNTRGRQGPHFYPVILYP*QRFGLCYLTL
LMFLRWLRGSLHLFRLTLISSVSVIPNVLRMCSD
KTLPITSVNKNLQKTSQIPELFLIPVQRSK*DP
ESIKM*FKVAYD*LTVM*TVSFFF*FNKKYIYF
*SIF*FSTSKQSHSISEMTLTSTL*NPYSFSIIF
FGRSFSPRSSRISLYMGTHTHKCVQLYVCMYTH
MHV*NSAVLGQVAWSRA*DGDLDA*DILRVGSE
GKGVREKEEGKKRVGQGLVSTGA*IAPPR*FDLE
SREPAFEPW*VNSGLPWEVG
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