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Sequences

Sequence BCAS1.eAug05

AGAATAATAATTCCATCATGAGTTTCTTTAAAACTCTGGTTTCACCTAACAAAGCTGAAACAAAAAAGGACCCAGAAGACACGGGTGCTGAAAAGTCACC CACCACTTCAGCTGACCTTAAGTCAGACAAAGCCAACTTTACATCCCAGGAGACCCAAGGGGCTGGCAAGAATTCCAAAGGATGCAACCCATCGGGGCAC ACACAGTCCGTGACAACCCCTGAACCTGCGAAGGAAGGCACCAAGGAGAAATCAGGACCCACCTCTCTGCCTCTGGGCAAACTGTTTTGGAAAAAGTCAG TTAAAGAGGACTCAGTCCCCACAGGTGCGGAGGAGAATTCAGTGAAAGGGGATGGAGGGATCACCCACTCAGAAGAAATAAATGGGAAAGACTCCAGCTG CCAAACATCAGACTCCACAGAAAAGACTATCACACCGCCAGAGCCTGAACCAACAGGAGCACCACAGAAGGGTAAAGAGGGCTCCTCGAAGGACAAGAAG TCAGCAGCCGAGATGAACAAGCAGAAGAGCAACAAGCAGGAAGCCAAAGAACCAGCCCAGTGCACAGAGCAGGCCACGGTGGACACGAACTCACTGCAGA ATGGGGACAAGCTCCAAAAGAGACCTGAGAAGCGGCAGCAGTCCCTTGGGGGCTTCTTTAAAGGCCTGGGACCAAAGCGGATGTTGGATGCTCAAGTGCA AACAGACCCAGTATCCATCGGACCAGTTGGCAAATCCAAGTAAACAAATCAGCACGGTTCCCACCAGGTTCTCCTGCCACCAAGATGTGTTCTCCTTACT CCATCTCCTCCCCAAACACGCTCCATGTATATATTCTTCTGATGGCCAGCAAATGAAATTCTGCCTAGAAATTAAGCCCGAGCTGTTGTATATTGAGGTG TATTATTTACGTCTCTGGTCCAGTCTTTTCTGGCAAATAACAGTAAAGATGGTTTAGCAGGTCACCTAGTTGGGTCAGAAGAGTCGATGATCACCAAGCA GGAAAGGGAGGGAATAGAGGAATGTGTTCGGGTTAAGTGATGAAAATGGCAGTGGTGGCCGGGCGTGGTGGCTCTCGCCTGTAATCTCAGCACTTTGGGA GGCCGAGGCAGGTGGATCACCTGAGGTCAGGAGTTCAAGACTAGCCTGGCCAACATCATGAAACCCCGTCTCTACTAAAAATACAAAAATTAGCCAGGCA TGGTGGCACACACCTGTAGTCCCAGCTACTCGGGAGCCCAACGCACGAGAACCGCTTGTACCCAGGAGGTGGAGGTTGCAGTGAGCCGAAGTTGCACCAT TGCACTCCACCCTGGGCGACAGAGCAAGATTCTATCAAAAAAAAAAAAAGGCAGTGGCAAGTAAGTTATAGAAGAGAAATGCTGCTAGAAGGAATTAAGC GTTGTAGTAAATGCGTGCTTATCCTCTAAGCTTGAAGAAGGGAGACGAAAATCCATTTGTTTAAATTCACATCTCAAGGAGGGAGAACCCGGGCTGTGTT GGGTGGTTGCCAATTTCCTAGAACGGAATGTGTGGGGTATAGAAAAAGGAATGAATAAGCGTTGTTTTTCAAATAGGGTCCTTGTAAGTTATTGATGAGA GGGAAAAGATTGACTGGGGAGGGCTTAAAATGATTTGGGAAAACAATTGCTTTTGAGGCTCAGTGACAACGGCAAAGATTACAACTTAAAAAAAAAAAAT AAATAAAAAATAAAGGAAGTTGCACGGTTATTTTGCAACACAAGGGGGCGGCAAGGTCCCCATTTTTATCCTGTAATACTGTATCCCTAACAAAGATTTG GTCTCTGCTATCTTACATTATTAATGTTTCTCAGATGGCTGAGGGGCTCGCTTCATCTGTTCCGTCTGACACTTATCTCAAGTGTGTCTGTCATTCCTAA TGTTCTCAGGATGTGCTCTGATAAAACCCTCCCCATAACCTCAGTTAATAAAAATTTACAGAAGACTTCTCAAATACCTGAGTTGTTTTTAATACCTGTA CAAAGGAGTAAATAGGACCCTGAGTCTATTAAAATGTAATTCAAAGTAGCATATGATTGACTGACAGTCATGTAAACTGTATCTTTCTTTTTCTGATTTA ATAAAAAATACATTTACTTCTAAAGTATCTTTTAATTTTCAACTTCAAAGCAGTCCCACTCTATATCTGAGATGACTCTAACTTCTACATTATAAAATCC ATACTCTTTCTCCATTATCTTCTTTGGAAGATCATTCTCTCCCAGATCTTCCCGGATTTCCTTGTATATGGGCACACACACACACAAATGTGTGCAGTTG TATGTTTGCATGTACACACACATGCATGTATAGAATAGTGCTGTCTTAGGCCAGGTTGCTTGGAGCAGAGCCTGAGATGGGGATTTGGACGCATAGGATA TACTGAGAGTGGGCTCCGAGGGAAAGGGAGTAAGAGAAAAAGAAGAGGGCAAGAAAAGAGTTGGGCAAGGGTTGGTTTCAACTGGAGCATGAATTGCACC ACCCAGATAGTTTGACCTTGAGTCAAGGGAGCCAGCCTTTGAACCCTGGTAGGTGAATTCTGGGCTGCCCTGGGAGGTGGGGGG

BCAS1.F2 (forward primer)
BCAS1.R1 (reverse primer)
Mutually exclusive exon skipping

Frame 0

RIIIPS*VSLKLWFHLTKLKQKRTQKTRVLKSH PPLQLTLSQTKPTLHPRRPKGLARIPKDATHRGT HSP*QPLNLRRKAPRRNQDPPLCLWANCFGKSQ LKRTQSPQVRRRIQ*KGMEGSPTQKK*MGKTPA AKHQTPQKRLSHRQSLNQQEHHRRVKRAPRRTRS QQPR*TSRRATSRKPKNQPSAQSRPRWTRTHCR MGTSSKRDLRSGSSPLGASLKAWDQSGCWMLKC KQTQYPSDQLANPSKQISTVPTRFSCHQDVFSLL HLLPKHAPCIYSSDGQQMKFCLEIKPELLYIEV YYLRLWSSLFWQITVKMV*QVT*LGQKSR*SPS RKGRE*RNVFGLSDENGSGGRAWWLSPVISALWE AEAGGSPEVRSSRLAWPTS*NPVSTKNTKISQA WWHTPVVPATREPNAREPLVPRRWRLQ*AEVAP LHSTLGDRARFYQKKKKAVASKL*KRNAARRN*A L**MRAYPLSLKKGDENPFV*IHISRRENPGCV GWLPIS*NGMCGV*KKE*ISVVFQIGSL*VIDE REKIDWGGLKMIWENNCF*GSVTTAKITT*KKKI NKK*RKLHGYFATQGGGKVPIFIL*YCIPNKDL VSAILHY*CFSDG*GARFICSV*HLSQVCLSFL MFSGCALIKPSP*PQLIKIYRRLLKYLSCF*YLY KGVNRTLSLLKCNSK*HMID*QSCKLYLSFSDL IKNTFTSKVSFNFQLQSSPTLYLR*L*LLHYKI HTLSPLSSLEDHSLPDLPGFPCIWAHTHTNVCSC MFACTHTCMYRIVLS*ARLLGAEPEMGIWTHRI Y*EWAPRERE*EKKKRARKELGKGWFQLEHELH HPDSLTLSQGSQPLNPGR*ILGCPGRWG

Frame 1

E**FHHEFL*NSGFT*QS*NKKGPRRHGC*KVT HHFS*P*VRQSQLYIPGDPRGWQEFQRMQPIGA HTVRDNP*TCEGRHQGEIRTHLSASGQTVLEKVS *RGLSPHRCGGEFSERGWRDHPLRRNKWERLQL PNIRLHRKDYHTARA*TNRSTTEG*RGLLEGQE VSSRDEQAEEQQAGSQRTSPVHRAGHGGHELTAE WGQAPKET*EAAAVPWGLL*RPGTKADVGCSSA NRPSIHRTSWQIQVNKSARFPPGSPATKMCSPY SISSPNTLHVYILLMASK*NSA*KLSPSCCILRC IIYVSGPVFSGK*Q*RWFSRSPSWVRRVDDHQA GKGGNRGMCSG*VMKMAVVAGRGGSRL*SQHFG RPRQVDHLRSGVQD*PGQHHETPSLLKIQKLARH GGTHL*SQLLGSPTHENRLYPGGGGCSEPKLHH CTPPWATEQDSIKKKKRQWQVSYRREMLLEGIK RCSKCVLIL*A*RRETKIHLFKFTSQGGRTRAVL GGCQFPRTECVGYRKRNE*ALFFK*GPCKLLMR GKRLTGEGLK*FGKTIAFEAQ*QRQRLQLKKKK *IKNKGSCTVILQHKGAARSPFLSCNTVSLTKIW SLLSYIINVSQMAEGLASSVPSDTYLKCVCHS* CSQDVL**NPPHNLS**KFTEDFSNT*VVFNTC TKE*IGP*VY*NVIQSSI*LTDSHVNCIFLFLI* *KIHLLLKYLLIFNFKAVPLYI*DDSNFYIIKS ILFLHYLLWKIILSQIFPDFLVYGHTHTQMCAV VCLHVHTHACIE*CCLRPGCLEQSLRWGFGRIGY TESGLRGKGSKRKRRGQEKSWARVGFNWSMNCT TQIV*P*VKGASL*TLVGEFWAALGGGG

Frame 2

NNNSIMSFFKTLVSPNKAETKKDPEDTGAEKSP TTSADLKSDKANFTSQETQGAGKNSKGCNPSGH TQSVTTPEPAKEGTKEKSGPTSLPLGKLFWKKS VKEDSVPTGAEENSVKGDGGITHSEEINGKDSSC QTSDSTEKTITPPEPEPTGAPQKGKEGSSKDKK SAAEMNKQKSNKQEAKEPAQCTEQATVDTNSLQ NGDKLQKRPEKRQQSLGGFFKGLGPKRMLDAQVQ TDPVSIGPVGKSK*TNQHGSHQVLLPPRCVLLT PSPPQTRSMYIFF*WPANEILPRN*ARAVVY*G VLFTSLVQSFLANNSKDGLAGHLVGSEESMITKQ EREGIEECVRVK**KWQWWPGVVALACNLSTLG GRGRWIT*GQEFKTSLANIMKPRLY*KYKN*PG MVAHTCSPSYSGAQRTRTACTQEVEVAVSRSCTI ALHPGRQSKILSKKKKGSGK*VIEEKCC*KELS VVVNACLSSKLEEGRRKSICLNSHLKEGEPGLC WVVANFLERNVWGIEKGMNKRCFSNRVLVSY**E GKD*LGRA*NDLGKQLLLRLSDNGKDYNLKKKN K*KIKEVARLFCNTRGRQGPHFYPVILYP*QRF GLCYLTLLMFLRWLRGSLHLFRLTLISSVSVIPN VLRMCSDKTLPITSVNKNLQKTSQIPELFLIPV QRSK*DPESIKM*FKVAYD*LTVM*TVSFFF*F NKKYIYF*SIF*FSTSKQSHSISEMTLTSTL*NP YSFSIIFFGRSFSPRSSRISLYMGTHTHKCVQL YVCMYTHMHV*NSAVLGQVAWSRA*DGDLDA*D ILRVGSEGKGVREKEEGKKRVGQGLVSTGA*IAP PR*FDLESREPAFEPW*VNSGLPWEVG

Sequence BCAS1.cAug05

GGTGGCAGCTGGACCGGGGCAGGACACAGATAAAACCCCAGGGCACGCCCCGGCCCAAGACAAGGTCCTCTCTGCCGCCAGGGATCCCACGCTTCTCCCA CCTGAGACAGGGGGAGCAGGAGGAGAAGCTCCCTCCAAGCCCAAGGACTCCAGCTTTTTTGACAAATTCTTCAAGCTGGACAAGGGACAGGAAAAGGTGC CAGGTGACAGCCAACAGGAAGCCAAGAGGGCAGAGCATCAAGACAAGGTGGATGAGGTTCCTGGCTTATCAGGGCAGTCCGATGATGTCCCTGCAGGGAA GGACATAGTTGACGGCAAGGAAAAAGAAGGACAAGAACTTGGAACTGCGGATTGCTCTGTCCCTGGGGACCCAGAAGGACTGGAGACTGCAAAGGACGAT TCCCAGGCAGCAGCTATAGCAGAGAATAATAATTCCATCATGAGTTTCTTTAAAACTCTGGTTTCACCTAACAAAGCTGAAACAAAAAAGGACCCAGAAG ACACGGCATCGAAAGCAGAAAGTGTCTGTGATGGACAAGCTGGTCAGAAGACATCCGAGATCCAGGCTAGAGGCACCAAGAAAAAGCACCTGGATAGCCC CAGGCTAGGACTCGCCTTTAGAAAATTCTTTAGGCATAAGGGTGCTGAAAAGTCACCCACCACTTCAGCTGACCTTAAGTCAGACAAAGCCAACTTTACA TCCCAGGAGACCCAAGGGGCTGGCAAGAATTCCAAAGGATGCAACCCATCGGGGCACACACAGTCCGTGACAACCCCTGAACCTGCGAAGGAAGGCACCA AGGAGAAATCAGGACCCACCTCTCTGCCTCTGGGCAAACTGTTTTGGAAAAAGGTGGTGTGTGAGTCACCAGTAGAGATTATAAAGTCCAAGGAAGTAGA ATCAGCCTTACAAACAGTGGACCTCAACGAAGGAGATGCTGCACCTGAACCCACAGAAGCGAAACTCAAAAGAGAAGAAAGCAAACCAAGAACCTCTCTG ATGGCGTTTCTCAGACAAATGACATCAGACTCCACAGAAAAGACTATCACACCGCCAGAGCCTGAACCAACAGGAGCACCACAGAAGGGTAAAGAGGGCT CCTCGAAGGACAAGAAGTCAGCAGCCGAGATGAACAAGCAGAAGAGCAACAAGCAGGAAGCCAAAGAACCAGCCCAGTGCACAGAGCAGGCCACGGTGGA CACGAACTCACTGCAGAATGGGGACAAGCTCCAAAAGAGACCTGAGAAGCGGCAGCAGTCCCTTGGGGGCTTCTTTAAAGGCCTGGGACCAAAGCGGATG TTGGATGCTCAAGTGCAAACAGACCCAGTATCCATCGGACCAGTTGGCAAATCCAAGTACACAGGGAAGTGTGTCTTCTCCCATGTCAAGAAGAAGTGGC CTTTCCAGGAATGGAGCTATTGAGCTGAAATGGCTGCCACTGCGCGTACACACACACACGCGCACACACACACACACACACACACCTCCCTTCCAGTTCC AGCTGACGGTGACTTTTTGACTGGTAACCATAAAAGAATTCATTCAAAAGGGCAGTTGTTCCCAGCAGCCAGTGAGGTCCTTCCTGCATTCGTTGTCTTG GAGAAGGAGGCACATAACAAAATAGAAACCAAAATGGGAAGGAAAAGAGAAAGAAAGTGAAATTCCTGGTCTTTGTTGTATGTATCCCCTTGCTTTTTAG ATAAATTATCAGGGGTTTAAACACACACTCTCTCCCCTCCCTCCTCCCACTTCACACACACTAGATTGTTCTTGTCTGTTTGCAAACAGTCCAGAAGCAC TGGGGTCTCATTAAATTAGATTCTAAGTGTCTTAAAGAATTAGATTTCAAGAAACCAAGAATCTGTGTCCTTTCCCTTCTCAGAGTGCCTGGAAGGGTGC AGATTTCTCCAATCTGAATCTGACAATGTTATTTCTTATGTCAGATAATCCCAAGTAGATCTTATACCTTAAAAAAATAAAATCAGCTACACTACCTACC TTACAGGGAAGGAAAAAGAACTAATTCCAATTATTAACTAACCCAAGATACAAACACAGGGGAAACTAGGGAATGTGCTTTGCAGCAATGTAAGATTATA GGGGTTGGTTTTAACCATCCTTTGAATGTCCCTCTTTAAGCCTTTTTAAAGGGCAATTTTGCACGGTGAGTAGAATTAGGCTTCTCCGTGGTCAAGATTT TGTTCACTCTATCAGTGCAAATTAAGTGGCACCGGCCCACTGGAAATTATAGTCTCCATTAGGAAGAAAAGAAATTACTTACCGAGCAGCAAATTCTCTA AATTTGTAATTCAGATCTTTAGTGCAGAATGCCGGTCAGGATCCAGCCCTTTGCATAAATTGGTTTTCTGGGGAGTTTAACCCCTTAGCCTCTGTTGCCC TTTTCTTGAATGGCATTCACGCAACCCTCTCCATCTCCTGGCCCTTTAACTGGCCCCGGCAGAAGTTGCAGTCCACAATTTCACAGAAGTAAGGATAATA CATTATCACACATTACCGTTTATAAAGTGCTCTGGGTACAATCTGTAATTTAAAGCTGTATTTCCCTCACTGAACTTAAGTGCTTCCTTTGTTACATTTC ATTACCGGGGGACTGGCATGCACCTGTATAGATTTTTTTTTTTTTTTTCATTTTTTGTTTTGTTTTGTTTTTCCAAATAAGCACTTCTTGACAAAATGGC CCCCAGCTGCACTTCTGCTAATGACAGCCATGGAAGGGCTGGTTCCTTACGTGATCTACTCTCTCAGAAGCTTGCTTTTGTCAAAATGCAGCCACGTGGG GGAGCTGCAGGACCTGGGGTTTTACAATGCCTAGCCCTTTTTCCCTGATTGCCTAACCGGGAAACTGGGCCAAAGCAGCCTGAGAGTGTATTTGTTGATT AACTTCTAGAAAGCAGTCAAGTTTAATTAGGAGAACTAGAACGAGAAAGCTGAACTGGGCAAGTCAGTTGCCTTCTATGATTCTCAGTCCTCCAAATGAA GATAAGGTTGGTTGGTTTGTGTTTCCATCCTTATCTGCTCTATCTTGGAAGAAGCTCAAGGAAGATTGTGTGATTGCAAAACGTTTCAGTTGGTGTCATT GCAATGCATTTAAGTAATCCTTACTGGCCCATCCTGCTCCACAAAGCAGCCTTACCTCTCATCTTCTCCACTCCCAACTAAAGAGCAAGTGATTTAAACT CTGAGTGTCCTCCCTCAACAATGGGCTAATAATAGAGCCTACTCGTGGAGTTGCTGCCAGCATGCAGTGAGATGGTGCATACCAAACATTTAGTGCAGTG GCTGGCACATTGTAAGCACTTAGGAAAGGCAAGCTATTCTATCATGGGGAACTTTTAGCACCAAGTAACAGAAAACCCCTACTTGAATTGGCAGATAATC GTAACAATAAGGGTATTTATCATTTCATATAATAGGTGTTGTATAACATCTTTGGCTTTGATTCTCTGAGACTCTCTTCCAGCTAACAACGATAATGAGG AAATGTATCGTTTCATATAACTGGTGTTAAGTCTCATAGCCTGGCTCTGGTTCTCTGACACTCCATTCTGCCATGTTCCATGGGTTGACTTCATCCTAAA ACTGTGCTGAATGATGGATGCAGTTCCAGGCAAGGGGATTATGGGAATACCATAATCAGTTTCAATGAATCATTTTGGTGAAATCATGTTGAGAAGGCAA TACAATGATACAGTAGACAGTAAAGCCATTATTGGTATCAGTGGCTTTAAGGCTAACCATTCCCCAAACTAACTGTGTCCCTTCAATTCACACCCATGGG ATTTGTGTAATGACGGCCCCCACCTTCCAAAGCAGTGCCACTAGAATGTGAACTTATCAAGATCTGAACCTTCTTGTCTTGCTCATTCATTGTTCTATTG TCAGTGTCTAGCACAATGCCTGGACATGGTAGATACTCAACAAATATTGGTTGAATGGATAAATTGCTAAACAAATATCCTGTGGCCACACCCATTGTGG ATTGCCTTATCCTCTAGTGTGAATTTGTTTTAGCTCTGAATGTCTGATCACCCTAACCAGACATCTACCCTTCCCAGGTAACCAGCCCAGTGGTTCTAAA AGTACAGTCCCAGGACCAGCAGCCTCAGCACCACCTGAGAACATGTTAGGAATGCAAACTCTCAAGCCCCACTCCATACCCGCAGAATCAAAAACTCTTG GGGTGGGACCCAGTAACCTGTGTTTTAGCAGCCTCTCAGGAGGTTCCAATGAAGGAGAAAATTTGAAAACCACTGGTCCAGGTAGAGCTACCACTTCACT GAGCATCAACAGCATGTTTGGTCCAAAGACTGGCCCTGAAGATGCTTAGATAAAAAAAGCTAACATTGAACCTCACTATATGCCAGGTATTGTGTGTCAC ATGACTTAACTTTGTTCACTGCTTATTTGGCAGCTGAGGAAACTAAGGCTCACAGAGAAAAAAACAACTTTGCCCAAGAACACACATGGCAGAGCCAAGG TTGAAATCCAACCTAAGTACATAAAAGCTTGCTTTCATGTCTGTGGGAGAGACAGGCAGGCAAAAAGATAATAATAATACAATGTAAGAGAAGCAAATGC AGGGTGTCATGGGACTCAGGGGAAGGCACTGGCGCAAGACCACAGTGGGTGGAGGTTGGCCACTTTGGGGAGGTGACAGTTGAGTTAACTCACACAGAAT GAATAAGCGTTGGACAAAAGGGCTCCCAGAAGAACAGCAAGTGCAAGAGCCCAGGGTCCACAGAGAGAATGGCAGGTAGCTTAGCATGGCTGAAGTGAGA CTGTGAGGAGAACGGATAAGAGGATGGAAGCTGGGGAGGTGGCACAGATGGATCTCAAGGACTTGGAGTAAAAAGTCCATAGTAACGAGTTTAAATTGTC TCTTGACGGCAATGGGGAGCCATGGAAGATATTTGAGCTGGAGGGTAACATAGATTTATATTTTGGCTGCAGGTAGGATCTAATACAAAAGCAATTCAAC TAATATTCACTAAGTGACCTGAGTTATCTGTGAGATATTTATTATAAGGTGTTAAACTTCTTTGAAGATTGTAAGAAAGAGATAAAACCTCTAAATGGTT TTGACCCTTCAAAGGACATTTAAATGGTTATTTCTAAGTGTCATAAACATGGACTTGGGATTTGGCCTCATCATTCACCCAGATGATTCATACCCATGAT CCCAGGCTGTGAGGTCTTGAGCAAATTACTTCTTCTCAGAGCCTTAGTTTGTTCCTCTGTGGAATGGGAATGCTAACAGCACTTACCTCGGAAAGTTGCT GAGAATAAACGAGACCGTGCATTTAGAGCATGTAGGCACCGTGTCTGGCAGGTAATGAGTGCTCAGTCAGCTTATTGATGAGGATGCAGATGACCTCAGC CTGAGATAGAACAGATGTGTTCTCTTTCATGGACTGAAGGCCACCCAGACTTTGCGACATCCTTAAACTTTGCCCCAGTTTCTCCTTATCCTCATAAGCA GTTGGCTGGCTGGCTTATCTAGAAAAAAGAAAGGAGGGAGGAGAAAAAGCAAAGAAAAATGTTCATTTTCCCTGCTGTTCACCCCATTCTAAAGTAAGGC AGAATTTCTGGAGAGGGGGCTCAGGTCCACCCTTGGCTATCCTTGGTTGTACAGTTGCATCTACTTATAACGGTGGCCAAGTCGCCATGGCAGTAGGTGC TGTGGCTGCCCTGCCCCATCGTCTTCAGACTACCCGAGTTTACCTGCAGCACTGACAGCTTCCCACCCCAAAACTCCTCGTTCTTTGCTTTTCCTCTCGG TGGCTTTCTCCAGGGGCTGTAGGAGCTTGTTTAGCCCACCTGCATGGCATTTTGAAAATACCTGAAATTTTATGCCCCGAAGGACAATTCTCAATCAGTG AAAGATGGGAGAGGAAAGGTAAACACCTCAGCTGCCCCATCTCTCAGTGGGACAATGTTGAGGGGTCGTCTTTGTGTTCTCTCAAGGGTCCTCAGCAAGA CCGAGCCACCCGTCACCCACAGCAGTAACATACTCATGAGCACGCCCCCCCACCCCACCTTTTGTGCTTCCTGGGATCACTTCCCAAATAAACTACTTGC ACCTA

BCAS1.F2 (forward primer)
BCAS1.R1 (reverse primer)
Mutually exclusive exon skipping

Frame 0

GGSWTGAGHR*NPRARPGPRQGPLCRQGSHASP T*DRGSRRRSSLQAQGLQLF*QILQAGQGTGKGA R*QPTGSQEGRASRQGG*GSWLIRAVR*CPCRE GHS*RQGKRRTRTWNCGLLCPWGPRRTGDCKGR FPGSSYSRE**FHHEFL*NSGFT*QS*NKKGPRR HGIESRKCL*WTSWSEDIRDPG*RHQEKAPG*P QARTRL*KIL*A*GC*KVTHHFS*P*VRQSQLY IPGDPRGWQEFQRMQPIGAHTVRDNP*TCEGRHQ GEIRTHLSASGQTVLEKGGV*VTSRDYKVQGSR ISLTNSGPQRRRCCT*THRSETQKRRKQTKNLS DGVSQTNDIRLHRKDYHTARA*TNRSTTEG*RGL LEGQEVSSRDEQAEEQQAGSQRTSPVHRAGHGG HELTAEWGQAPKET*EAAAVPWGLL*RPGTKAD VGCSSANRPSIHRTSWQIQVHREVCLLPCQEEVA FPGMELLS*NGCHCAYTHTRAHTHTHTHLPSSS S*R*LFDW*P*KNSFKRAVVPSSQ*GPSCIRCL GEGGT*QNRNQNGKEKRKKVKFLVFVVCIPLLFR *IIRGLNTHSLPSLLPLHTH*IVLVCLQTVQKH WGLIKLDSKCLKELDFKKPRICVLSLLRVPGRV QISPI*I*QCYFLCQIIPSRSYTLKK*NQLHYLP YREGKRTNSNY*LTQDTNTGETRECALQQCKII GVGFNHPLNVPL*AFLKGNFAR*VELGFSVVKI LFTLSVQIKWHRPTGNYSLH*EEKKLLTEQQIL* ICNSDL*CRMPVRIQPFA*IGFLGSLTP*PLLP FS*MAFTQPSPSPGPLTGPGRSCSPQFHRSKDN TLSHITVYKVLWVQSVI*SCISLTELKCFLCYIS LPGDWHAPV*IFFFFFIFCFVLFFQISTS*QNG PQLHFC**QPWKGWFLT*STLSEACFCQNAATW GSCRTWGFTMPSPFSLIA*PGNWAKAA*ECIC*L TSRKQSSLIRRTRTRKLNWASQLPSMILSPPNE DKVGWFVFPSLSALSWKKLKEDCVIAKRFSWCH CNAFK*SLLAHPAPQSSLTSHLLHSQLKSK*FKL *VSSLNNGLIIEPTRGVAASMQ*DGAYQTFSAV AGTL*ALRKGKLFYHGELLAPSNRKPLLELADN RNNKGIYHFI**VLYNIFGFDSLRLSSS*QR**G NVSFHITGVKSHSLALVL*HSILPCSMG*LHPK TVLNDGCSSRQGDYGNTIISFNESFW*NHVEKA IQ*YSRQ*SHYWYQWL*G*PFPKLTVSLQFTPMG FV**RPPPSKAVPLECELIKI*TFLSCSFIVLL SVSSTMPGHGRYSTNIG*MDKLLNKYPVATPIV DCLIL*CEFVLALNV*SP*PDIYPSQVTSPVVLK VQSQDQQPQHHLRTC*ECKLSSPTPYPQNQKLL GWDPVTCVLAASQEVPMKEKI*KPLVQVELPLH *ASTACLVQRLALKMLR*KKLTLNLTICQVLCVT *LNFVHCLFGS*GN*GSQRKKQLCPRTHMAEPR LKSNLST*KLAFMSVGETGRQKDNNNTM*EKQM QGVMGLRGRHWRKTTVGGGWPLWGGDS*VNSHRM NKRWTKGLPEEQQVQEPRVHRENGR*LSMAEVR L*GERIRGWKLGRWHRWISRTWSKKSIVTSLNC LLTAMGSHGRYLSWRVT*IYILAAGRI*YKSNST NIH*VT*VICEIFIIRC*TSLKIVRKR*NL*MV LTLQRTFKWLFLSVINMDLGFGLIIHPDDSYP* SQAVRS*ANYFFSEP*FVPLWNGNANSTYLGKLL RINETVHLEHVGTVSGR**VLSQLIDEDADDLS LR*NRCVLFHGLKATQTLRHP*TLPQFLLILIS SWLAGLSRKKKGGRRKSKEKCSFSLLFTPF*SKA EFLERGLRSTLGYPWLYSCIYL*RWPSRHGSRC CGCPAPSSSDYPSLPAALTASHPKTPRSLLFLS VAFSRGCRSLFSPPAWHFENT*NFMPRRTILNQ* KMGEER*TPQLPHLSVGQC*GVVFVFSQGSSAR PSHPSPTAVTYS*ARPPTPPFVLPGITSQINYL HL

Frame 1

VAAGPGQDTDKTPGHAPAQDKVLSAARDPTLLP PETGGAGGEAPSKPKDSSFFDKFFKLDKGQEKV PGDSQQEAKRAEHQDKVDEVPGLSGQSDDVPAGK DIVDGKEKEGQELGTADCSVPGDPEGLETAKDD SQAAAIAENNNSIMSFFKTLVSPNKAETKKDPE DTASKAESVCDGQAGQKTSEIQARGTKKKHLDSP RLGLAFRKFFRHKGAEKSPTTSADLKSDKANFT SQETQGAGKNSKGCNPSGHTQSVTTPEPAKEGT KEKSGPTSLPLGKLFWKKVVCESPVEIIKSKEVE SALQTVDLNEGDAAPEPTEAKLKREESKPRTSL MAFLRQMTSDSTEKTITPPEPEPTGAPQKGKEG SSKDKKSAAEMNKQKSNKQEAKEPAQCTEQATVD TNSLQNGDKLQKRPEKRQQSLGGFFKGLGPKRM LDAQVQTDPVSIGPVGKSKYTGKCVFSHVKKKW PFQEWSY*AEMAATARTHTHAHTHTHTHTSLPVP ADGDFLTGNHKRIHSKGQLFPAASEVLPAFVVL EKEAHNKIETKMGRKRERK*NSWSLLYVSPCFL DKLSGV*THTLSPPSSHFTHTRLFLSVCKQSRST GVSLN*ILSVLKN*ISRNQESVSFPFSECLEGC RFLQSESDNVISYVR*SQVDLIP*KNKISYTTY LTGKEKELIPIIN*PKIQTQGKLGNVLCSNVRL* GLVLTIL*MSLFKPF*RAILHGE*N*ASPWSRF CSLYQCKLSGTGPLEIIVSIRKKRNYLPSSKFS KFVIQIFSAECRSGSSPLHKLVFWGV*PLSLCCP FLEWHSRNPLHLLAL*LAPAEVAVHNFTEVRII HYHTLPFIKCSGYNL*FKAVFPSLNLSASFVTF HYRGTGMHLYRFFFFFSFFVLFCFSK*ALLDKMA PSCTSANDSHGRAGSLRDLLSQKLAFVKMQPRG GAAGPGVLQCLALFP*LPNRETGPKQPESVFVD *LLESSQV*LGELERES*TGQVSCLL*FSVLQMK IRLVGLCFHPYLLYLGRSSRKIV*LQNVSVGVI AMHLSNPYWPILLHKAALPLIFSTPN*RASDLN SECPPSTMG***SLLVELLPACSEMVHTKHLVQW LAHCKHLGKASYSIMGNF*HQVTENPYLNWQII VTIRVFIISYNRCCITSLALIL*DSLPANNDNE EMYRFI*LVLSLIAWLWFSDTPFCHVPWVDFILK LC*MMDAVPGKGIMGIP*SVSMNHFGEIMLRRQ YNDTVDSKAIIGISGFKANHSPN*LCPFNSHPW DLCNDGPHLPKQCH*NVNLSRSEPSCLAHSLFYC QCLAQCLDMVDTQQILVEWINC*TNILWPHPLW IALSSSVNLF*L*MSDHPNQTSTLPR*PAQWF* KYSPRTSSLSTT*EHVRNANSQAPLHTRRIKNSW GGTQ*PVF*QPLRRFQ*RRKFENHWSR*SYHFT EHQQHVWSKDWP*RCLDKKS*H*TSLYARYCVS HDLTLFTAYLAAEETKAHREKNNFAQEHTWQSQG *NPT*VHKSLLSCLWERQAGKKIIIIQCKRSKC RVSWDSGEGTGARPQWVEVGHFGEVTVELTHTE *ISVGQKGSQKNSKCKSPGSTERMAGSLAWLK*D CEENG*EDGSWGGGTDGSQGLGVKSP**RV*IV S*RQWGAMEDI*AGG*HRFIFWLQVGSNTKAIQ LIFTK*PELSVRYLL*GVKLL*RL*ERDKTSKWF *PFKGHLNGYF*VS*TWTWDLASSFTQMIHTHD PRL*GLEQITSSQSLSLFLCGMGMLTALTSESC *E*TRPCI*SM*APCLAGNECSVSLLMRMQMTSA *DRTDVFSFMD*RPPRLCDILKLCPSFSLSS*A VGWLAYLEKRKEGGEKAKKNVHFPCCSPHSKVR QNFWRGGSGPPLAILGCTVASTYNGGQVAMAVGA VAALPHRLQTTRVYLQH*QLPTPKLLVLCFSSR WLSPGAVGACLAHLHGILKIPEILCPEGQFSIS ERWERKGKHLSCPISQWDNVEGSSLCSLKGPQQD RATRHPQQ*HTHEHAPPPHLLCFLGSLPK*TTC T

Frame 2

WQLDRGRTQIKPQGTPRPKTRSSLPPGIPRFSH LRQGEQEEKLPPSPRTPAFLTNSSSWTRDRKRC QVTANRKPRGQSIKTRWMRFLAYQGSPMMSLQG RT*LTARKKKDKNLELRIALSLGTQKDWRLQRTI PRQQL*QRIIIPS*VSLKLWFHLTKLKQKRTQK TRHRKQKVSVMDKLVRRHPRSRLEAPRKSTWIA PG*DSPLENSLGIRVLKSHPPLQLTLSQTKPTLH PRRPKGLARIPKDATHRGTHSP*QPLNLRRKAP RRNQDPPLCLWANCFGKRWCVSHQ*RL*SPRK* NQPYKQWTSTKEMLHLNPQKRNSKEKKANQEPL* WRFSDK*HQTPQKRLSHRQSLNQQEHHRRVKRA PRRTRSQQPR*TSRRATSRKPKNQPSAQSRPRW TRTHCRMGTSSKRDLRSGSSPLGASLKAWDQSGC WMLKCKQTQYPSDQLANPSTQGSVSSPMSRRSG LSRNGAIELKWLPLRVHTHTRTHTHTHTPPFQF QLTVTF*LVTIKEFIQKGSCSQQPVRSFLHSLSW RRRHITK*KPKWEGKEKESEIPGLCCMYPLAF* INYQGFKHTLSPLPPPTSHTLDCSCLFANSPEA LGSH*IRF*VS*RIRFQETKNLCPFPSQSAWKGA DFSNLNLTMLFLMSDNPK*ILYLKKIKSATLPT LQGRKKN*FQLLTNPRYKHRGN*GMCFAAM*DY RGWF*PSFECPSLSLFKGQFCTVSRIRLLRGQDF VHSISAN*VAPAHWKL*SPLGRKEITYRAANSL NL*FRSLVQNAGQDPALCINWFSGEFNPLASVA LFLNGIHATLSISWPFNWPRQKLQSTISQK*G*Y IITHYRL*SALGTICNLKLYFPH*T*VLPLLHF ITGGLACTCIDFFFFFHFLFCFVFPNKHFLTKW PPAALLLMTAMEGLVPYVIYSLRSLLLSKCSHVG ELQDLGFYNA*PFFPDCLTGKLGQSSLRVYLLI NF*KAVKFN*EN*NEKAELGKSVAFYDSQSSK* R*GWLVCVSILICSILEEAQGRLCDCKTFQLVSL QCI*VILTGPSCSTKQPYLSSSPLPTKEQVI*T LSVLPQQWANNRAYSWSCCQHAVRWCIPNI*CS GWHIVST*ERQAILSWGTFSTK*QKTPT*IGR*S *Q*GYLSFHIIGVV*HLWL*FSETLFQLTTIMR KCIVSYNWC*VS*PGSGSLTLHSAMFHGLTSS* NCAE*WMQFQARGLWEYHNQFQ*IILVKSC*EGN TMIQ*TVKPLLVSVALRLTIPQTNCVPSIHTHG ICVMTAPTFQSSATRM*TYQDLNLLVLLIHCSI VSV*HNAWTW*ILNKYWLNG*IAKQISCGHTHCG LPYPLV*ICFSSECLITLTRHLPFPGNQPSGSK STVPGPAASAPPENMLGMQTLKPHSIPAESKTL GVGPSNLCFSSLSGGSNEGENLKTTGPGRATTSL SINSMFGPKTGPEDA*IKKANIEPHYMPGIVCH MT*LCSLLIWQLRKLRLTEKKTTLPKNTHGRAK VEIQPKYIKACFHVCGRDRQAKR***YNVREANA GCHGTQGKALAQDHSGWRLATLGR*QLS*LTQN E*ALDKRAPRRTASARAQGPQREWQVA*HG*SE TVRRTDKRMEAGEVAQMDLKDLE*KVHSNEFKLS LDGNGEPWKIFELEGNIDLYFGCR*DLIQKQFN *YSLSDLSYL*DIYYKVLNFFEDCKKEIKPLNG FDPSKDI*MVISKCHKHGLGIWPHHSPR*FIPMI PGCEVLSKLLLLRALVCSSVEWEC*QHLPRKVA ENKRDRAFRACRHRVWQVMSAQSAY**GCR*PQ PEIEQMCSLSWTEGHPDFATSLNFAPVSPYPHKQ LAGWLI*KKERREEKKQRKMFIFPAVHPILK*G RISGEGAQVHPWLSLVVQLHLLITVAKSPWQ*V LWLPCPIVFRLPEFTCSTDSFPPQNSSFFAFPLG GFLQGL*ELV*PTCMAF*KYLKFYAPKDNSQSV KDGRGKVNTSAAPSLSGTMLRGRLCVLSRVLSK TEPPVTHSSNILMSTPPHPTFCASWDHFPNKLLA P