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Sequences

Sequence BCAS1.dAug05

GGTGGCAGCTGGACCGGGGCAGGACACAGATAAAACCCCAGGGCACGCCCCGGCCCAAGACAAGGTCCTCTCTGCCGCCAGGGATCCCACGCTTCTCCCA
CCTGAGACAGGGGGAGCAGGAGGAGAAGCTCCCTCCAAGCCCAAGGACTCCAGCTTTTTTGACAAATTCTTCAAGCTGGACAAGGGACAGGAAAAGGTGC
CAGGTGACAGCCAACAGGAAGCCAAGAGGGCAGAGCATCAAGACAAGGTGGATGAGGTTCCTGGCTTATCAGGGCAGTCCGATGATGTCCCTGCAGGGAA
GGACATAGTTGACGGCAAGGAAAAAGAAGGACAAGAACTTGGAACTGCGGATTGCTCTGTCCCTGGGGACCCAGAAGGACTGGAGACTGCAAAGGACGAT
TCCCAGGCAGCAGCTATAGCAGAGAATAATAATTCCATCATGAGTTTCTTTAAAACTCTGGTTTCACCTAACAAAGCTGAAACAAAAAAGGACCCAGAAG
ACACGGCATCGAAAGCAGAAAGTGTCTGTGATGGACAAGCTGGTCAGAAGACATCCGAGATCCAGGCTAGAGGCACCAAGAAAAAGCACCTGGATAGCCC
CAGGCTAGGACTCGCCTTTAGAAAATTCTTTAGGCATAAGGGTGCTGAAAAGTCACCCACCACTTCAGCTGACCTTAAGTCAGACAAAGCCAACTTTACA
TCCCAGGAGACCCAAGGGGCTGGCAAGAATTCCAAAGGATGCAACCCATCGGGGCACACACAGTCCGTGACAACCCCTGAACCTGCGAAGGAAGGCACCA
AGGAGAAATCAGGACCCACCTCTCTGCCTCTGGGCAAACTGTTTTGGAAAAAGACATCAGACTCCACAGAAAAGACTATCACACCGCCAGAGCCTGAACC
AACAGGAGCACC
  • BCAS1.F2 (forward primer)
  • BCAS1.R1 (reverse primer)
  • Multiple exon skipping

Frame 0

GGSWTGAGHR*NPRARPGPRQGPLCRQGSHASP
T*DRGSRRRSSLQAQGLQLF*QILQAGQGTGKGA
R*QPTGSQEGRASRQGG*GSWLIRAVR*CPCRE
GHS*RQGKRRTRTWNCGLLCPWGPRRTGDCKGR
FPGSSYSRE**FHHEFL*NSGFT*QS*NKKGPRR
HGIESRKCL*WTSWSEDIRDPG*RHQEKAPG*P
QARTRL*KIL*A*GC*KVTHHFS*P*VRQSQLY
IPGDPRGWQEFQRMQPIGAHTVRDNP*TCEGRHQ
GEIRTHLSASGQTVLEKDIRLHRKDYHTARA*T
NRST

Frame 1

VAAGPGQDTDKTPGHAPAQDKVLSAARDPTLLP
PETGGAGGEAPSKPKDSSFFDKFFKLDKGQEKV
PGDSQQEAKRAEHQDKVDEVPGLSGQSDDVPAGK
DIVDGKEKEGQELGTADCSVPGDPEGLETAKDD
SQAAAIAENNNSIMSFFKTLVSPNKAETKKDPE
DTASKAESVCDGQAGQKTSEIQARGTKKKHLDSP
RLGLAFRKFFRHKGAEKSPTTSADLKSDKANFT
SQETQGAGKNSKGCNPSGHTQSVTTPEPAKEGT
KEKSGPTSLPLGKLFWKKTSDSTEKTITPPEPEP
TGA

Frame 2

WQLDRGRTQIKPQGTPRPKTRSSLPPGIPRFSH
LRQGEQEEKLPPSPRTPAFLTNSSSWTRDRKRC
QVTANRKPRGQSIKTRWMRFLAYQGSPMMSLQG
RT*LTARKKKDKNLELRIALSLGTQKDWRLQRTI
PRQQL*QRIIIPS*VSLKLWFHLTKLKQKRTQK
TRHRKQKVSVMDKLVRRHPRSRLEAPRKSTWIA
PG*DSPLENSLGIRVLKSHPPLQLTLSQTKPTLH
PRRPKGLARIPKDATHRGTHSP*QPLNLRRKAP
RRNQDPPLCLWANCFGKRHQTPQKRLSHRQSLN
QQEH

Sequence BCAS1.eAug05

AGAATAATAATTCCATCATGAGTTTCTTTAAAACTCTGGTTTCACCTAACAAAGCTGAAACAAAAAAGGACCCAGAAGACACGGGTGCTGAAAAGTCACC
CACCACTTCAGCTGACCTTAAGTCAGACAAAGCCAACTTTACATCCCAGGAGACCCAAGGGGCTGGCAAGAATTCCAAAGGATGCAACCCATCGGGGCAC
ACACAGTCCGTGACAACCCCTGAACCTGCGAAGGAAGGCACCAAGGAGAAATCAGGACCCACCTCTCTGCCTCTGGGCAAACTGTTTTGGAAAAAGTCAG
TTAAAGAGGACTCAGTCCCCACAGGTGCGGAGGAGAATTCAGTGAAAGGGGATGGAGGGATCACCCACTCAGAAGAAATAAATGGGAAAGACTCCAGCTG
CCAAACATCAGACTCCACAGAAAAGACTATCACACCGCCAGAGCCTGAACCAACAGGAGCACCACAGAAGGGTAAAGAGGGCTCCTCGAAGGACAAGAAG
TCAGCAGCCGAGATGAACAAGCAGAAGAGCAACAAGCAGGAAGCCAAAGAACCAGCCCAGTGCACAGAGCAGGCCACGGTGGACACGAACTCACTGCAGA
ATGGGGACAAGCTCCAAAAGAGACCTGAGAAGCGGCAGCAGTCCCTTGGGGGCTTCTTTAAAGGCCTGGGACCAAAGCGGATGTTGGATGCTCAAGTGCA
AACAGACCCAGTATCCATCGGACCAGTTGGCAAATCCAAGTAAACAAATCAGCACGGTTCCCACCAGGTTCTCCTGCCACCAAGATGTGTTCTCCTTACT
CCATCTCCTCCCCAAACACGCTCCATGTATATATTCTTCTGATGGCCAGCAAATGAAATTCTGCCTAGAAATTAAGCCCGAGCTGTTGTATATTGAGGTG
TATTATTTACGTCTCTGGTCCAGTCTTTTCTGGCAAATAACAGTAAAGATGGTTTAGCAGGTCACCTAGTTGGGTCAGAAGAGTCGATGATCACCAAGCA
GGAAAGGGAGGGAATAGAGGAATGTGTTCGGGTTAAGTGATGAAAATGGCAGTGGTGGCCGGGCGTGGTGGCTCTCGCCTGTAATCTCAGCACTTTGGGA
GGCCGAGGCAGGTGGATCACCTGAGGTCAGGAGTTCAAGACTAGCCTGGCCAACATCATGAAACCCCGTCTCTACTAAAAATACAAAAATTAGCCAGGCA
TGGTGGCACACACCTGTAGTCCCAGCTACTCGGGAGCCCAACGCACGAGAACCGCTTGTACCCAGGAGGTGGAGGTTGCAGTGAGCCGAAGTTGCACCAT
TGCACTCCACCCTGGGCGACAGAGCAAGATTCTATCAAAAAAAAAAAAAGGCAGTGGCAAGTAAGTTATAGAAGAGAAATGCTGCTAGAAGGAATTAAGC
GTTGTAGTAAATGCGTGCTTATCCTCTAAGCTTGAAGAAGGGAGACGAAAATCCATTTGTTTAAATTCACATCTCAAGGAGGGAGAACCCGGGCTGTGTT
GGGTGGTTGCCAATTTCCTAGAACGGAATGTGTGGGGTATAGAAAAAGGAATGAATAAGCGTTGTTTTTCAAATAGGGTCCTTGTAAGTTATTGATGAGA
GGGAAAAGATTGACTGGGGAGGGCTTAAAATGATTTGGGAAAACAATTGCTTTTGAGGCTCAGTGACAACGGCAAAGATTACAACTTAAAAAAAAAAAAT
AAATAAAAAATAAAGGAAGTTGCACGGTTATTTTGCAACACAAGGGGGCGGCAAGGTCCCCATTTTTATCCTGTAATACTGTATCCCTAACAAAGATTTG
GTCTCTGCTATCTTACATTATTAATGTTTCTCAGATGGCTGAGGGGCTCGCTTCATCTGTTCCGTCTGACACTTATCTCAAGTGTGTCTGTCATTCCTAA
TGTTCTCAGGATGTGCTCTGATAAAACCCTCCCCATAACCTCAGTTAATAAAAATTTACAGAAGACTTCTCAAATACCTGAGTTGTTTTTAATACCTGTA
CAAAGGAGTAAATAGGACCCTGAGTCTATTAAAATGTAATTCAAAGTAGCATATGATTGACTGACAGTCATGTAAACTGTATCTTTCTTTTTCTGATTTA
ATAAAAAATACATTTACTTCTAAAGTATCTTTTAATTTTCAACTTCAAAGCAGTCCCACTCTATATCTGAGATGACTCTAACTTCTACATTATAAAATCC
ATACTCTTTCTCCATTATCTTCTTTGGAAGATCATTCTCTCCCAGATCTTCCCGGATTTCCTTGTATATGGGCACACACACACACAAATGTGTGCAGTTG
TATGTTTGCATGTACACACACATGCATGTATAGAATAGTGCTGTCTTAGGCCAGGTTGCTTGGAGCAGAGCCTGAGATGGGGATTTGGACGCATAGGATA
TACTGAGAGTGGGCTCCGAGGGAAAGGGAGTAAGAGAAAAAGAAGAGGGCAAGAAAAGAGTTGGGCAAGGGTTGGTTTCAACTGGAGCATGAATTGCACC
ACCCAGATAGTTTGACCTTGAGTCAAGGGAGCCAGCCTTTGAACCCTGGTAGGTGAATTCTGGGCTGCCCTGGGAGGTGGGGGG
  • BCAS1.F2 (forward primer)
  • BCAS1.R1 (reverse primer)
  • Multiple exon skipping

Frame 0

RIIIPS*VSLKLWFHLTKLKQKRTQKTRVLKSH
PPLQLTLSQTKPTLHPRRPKGLARIPKDATHRGT
HSP*QPLNLRRKAPRRNQDPPLCLWANCFGKSQ
LKRTQSPQVRRRIQ*KGMEGSPTQKK*MGKTPA
AKHQTPQKRLSHRQSLNQQEHHRRVKRAPRRTRS
QQPR*TSRRATSRKPKNQPSAQSRPRWTRTHCR
MGTSSKRDLRSGSSPLGASLKAWDQSGCWMLKC
KQTQYPSDQLANPSKQISTVPTRFSCHQDVFSLL
HLLPKHAPCIYSSDGQQMKFCLEIKPELLYIEV
YYLRLWSSLFWQITVKMV*QVT*LGQKSR*SPS
RKGRE*RNVFGLSDENGSGGRAWWLSPVISALWE
AEAGGSPEVRSSRLAWPTS*NPVSTKNTKISQA
WWHTPVVPATREPNAREPLVPRRWRLQ*AEVAP
LHSTLGDRARFYQKKKKAVASKL*KRNAARRN*A
L**MRAYPLSLKKGDENPFV*IHISRRENPGCV
GWLPIS*NGMCGV*KKE*ISVVFQIGSL*VIDE
REKIDWGGLKMIWENNCF*GSVTTAKITT*KKKI
NKK*RKLHGYFATQGGGKVPIFIL*YCIPNKDL
VSAILHY*CFSDG*GARFICSV*HLSQVCLSFL
MFSGCALIKPSP*PQLIKIYRRLLKYLSCF*YLY
KGVNRTLSLLKCNSK*HMID*QSCKLYLSFSDL
IKNTFTSKVSFNFQLQSSPTLYLR*L*LLHYKI
HTLSPLSSLEDHSLPDLPGFPCIWAHTHTNVCSC
MFACTHTCMYRIVLS*ARLLGAEPEMGIWTHRI
Y*EWAPRERE*EKKKRARKELGKGWFQLEHELH
HPDSLTLSQGSQPLNPGR*ILGCPGRWG

Frame 1

E**FHHEFL*NSGFT*QS*NKKGPRRHGC*KVT
HHFS*P*VRQSQLYIPGDPRGWQEFQRMQPIGA
HTVRDNP*TCEGRHQGEIRTHLSASGQTVLEKVS
*RGLSPHRCGGEFSERGWRDHPLRRNKWERLQL
PNIRLHRKDYHTARA*TNRSTTEG*RGLLEGQE
VSSRDEQAEEQQAGSQRTSPVHRAGHGGHELTAE
WGQAPKET*EAAAVPWGLL*RPGTKADVGCSSA
NRPSIHRTSWQIQVNKSARFPPGSPATKMCSPY
SISSPNTLHVYILLMASK*NSA*KLSPSCCILRC
IIYVSGPVFSGK*Q*RWFSRSPSWVRRVDDHQA
GKGGNRGMCSG*VMKMAVVAGRGGSRL*SQHFG
RPRQVDHLRSGVQD*PGQHHETPSLLKIQKLARH
GGTHL*SQLLGSPTHENRLYPGGGGCSEPKLHH
CTPPWATEQDSIKKKKRQWQVSYRREMLLEGIK
RCSKCVLIL*A*RRETKIHLFKFTSQGGRTRAVL
GGCQFPRTECVGYRKRNE*ALFFK*GPCKLLMR
GKRLTGEGLK*FGKTIAFEAQ*QRQRLQLKKKK
*IKNKGSCTVILQHKGAARSPFLSCNTVSLTKIW
SLLSYIINVSQMAEGLASSVPSDTYLKCVCHS*
CSQDVL**NPPHNLS**KFTEDFSNT*VVFNTC
TKE*IGP*VY*NVIQSSI*LTDSHVNCIFLFLI*
*KIHLLLKYLLIFNFKAVPLYI*DDSNFYIIKS
ILFLHYLLWKIILSQIFPDFLVYGHTHTQMCAV
VCLHVHTHACIE*CCLRPGCLEQSLRWGFGRIGY
TESGLRGKGSKRKRRGQEKSWARVGFNWSMNCT
TQIV*P*VKGASL*TLVGEFWAALGGGG

Frame 2

NNNSIMSFFKTLVSPNKAETKKDPEDTGAEKSP
TTSADLKSDKANFTSQETQGAGKNSKGCNPSGH
TQSVTTPEPAKEGTKEKSGPTSLPLGKLFWKKS
VKEDSVPTGAEENSVKGDGGITHSEEINGKDSSC
QTSDSTEKTITPPEPEPTGAPQKGKEGSSKDKK
SAAEMNKQKSNKQEAKEPAQCTEQATVDTNSLQ
NGDKLQKRPEKRQQSLGGFFKGLGPKRMLDAQVQ
TDPVSIGPVGKSK*TNQHGSHQVLLPPRCVLLT
PSPPQTRSMYIFF*WPANEILPRN*ARAVVY*G
VLFTSLVQSFLANNSKDGLAGHLVGSEESMITKQ
EREGIEECVRVK**KWQWWPGVVALACNLSTLG
GRGRWIT*GQEFKTSLANIMKPRLY*KYKN*PG
MVAHTCSPSYSGAQRTRTACTQEVEVAVSRSCTI
ALHPGRQSKILSKKKKGSGK*VIEEKCC*KELS
VVVNACLSSKLEEGRRKSICLNSHLKEGEPGLC
WVVANFLERNVWGIEKGMNKRCFSNRVLVSY**E
GKD*LGRA*NDLGKQLLLRLSDNGKDYNLKKKN
K*KIKEVARLFCNTRGRQGPHFYPVILYP*QRF
GLCYLTLLMFLRWLRGSLHLFRLTLISSVSVIPN
VLRMCSDKTLPITSVNKNLQKTSQIPELFLIPV
QRSK*DPESIKM*FKVAYD*LTVM*TVSFFF*F
NKKYIYF*SIF*FSTSKQSHSISEMTLTSTL*NP
YSFSIIFFGRSFSPRSSRISLYMGTHTHKCVQL
YVCMYTHMHV*NSAVLGQVAWSRA*DGDLDA*D
ILRVGSEGKGVREKEEGKKRVGQGLVSTGA*IAP
PR*FDLESREPAFEPW*VNSGLPWEVG

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