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Sequences

Sequence BCAS1.cAug05

GGTGGCAGCTGGACCGGGGCAGGACACAGATAAAACCCCAGGGCACGCCCCGGCCCAAGACAAGGTCCTCTCTGCCGCCAGGGATCCCACGCTTCTCCCA
CCTGAGACAGGGGGAGCAGGAGGAGAAGCTCCCTCCAAGCCCAAGGACTCCAGCTTTTTTGACAAATTCTTCAAGCTGGACAAGGGACAGGAAAAGGTGC
CAGGTGACAGCCAACAGGAAGCCAAGAGGGCAGAGCATCAAGACAAGGTGGATGAGGTTCCTGGCTTATCAGGGCAGTCCGATGATGTCCCTGCAGGGAA
GGACATAGTTGACGGCAAGGAAAAAGAAGGACAAGAACTTGGAACTGCGGATTGCTCTGTCCCTGGGGACCCAGAAGGACTGGAGACTGCAAAGGACGAT
TCCCAGGCAGCAGCTATAGCAGAGAATAATAATTCCATCATGAGTTTCTTTAAAACTCTGGTTTCACCTAACAAAGCTGAAACAAAAAAGGACCCAGAAG
ACACGGCATCGAAAGCAGAAAGTGTCTGTGATGGACAAGCTGGTCAGAAGACATCCGAGATCCAGGCTAGAGGCACCAAGAAAAAGCACCTGGATAGCCC
CAGGCTAGGACTCGCCTTTAGAAAATTCTTTAGGCATAAGGGTGCTGAAAAGTCACCCACCACTTCAGCTGACCTTAAGTCAGACAAAGCCAACTTTACA
TCCCAGGAGACCCAAGGGGCTGGCAAGAATTCCAAAGGATGCAACCCATCGGGGCACACACAGTCCGTGACAACCCCTGAACCTGCGAAGGAAGGCACCA
AGGAGAAATCAGGACCCACCTCTCTGCCTCTGGGCAAACTGTTTTGGAAAAAGGTGGTGTGTGAGTCACCAGTAGAGATTATAAAGTCCAAGGAAGTAGA
ATCAGCCTTACAAACAGTGGACCTCAACGAAGGAGATGCTGCACCTGAACCCACAGAAGCGAAACTCAAAAGAGAAGAAAGCAAACCAAGAACCTCTCTG
ATGGCGTTTCTCAGACAAATGACATCAGACTCCACAGAAAAGACTATCACACCGCCAGAGCCTGAACCAACAGGAGCACCACAGAAGGGTAAAGAGGGCT
CCTCGAAGGACAAGAAGTCAGCAGCCGAGATGAACAAGCAGAAGAGCAACAAGCAGGAAGCCAAAGAACCAGCCCAGTGCACAGAGCAGGCCACGGTGGA
CACGAACTCACTGCAGAATGGGGACAAGCTCCAAAAGAGACCTGAGAAGCGGCAGCAGTCCCTTGGGGGCTTCTTTAAAGGCCTGGGACCAAAGCGGATG
TTGGATGCTCAAGTGCAAACAGACCCAGTATCCATCGGACCAGTTGGCAAATCCAAGTACACAGGGAAGTGTGTCTTCTCCCATGTCAAGAAGAAGTGGC
CTTTCCAGGAATGGAGCTATTGAGCTGAAATGGCTGCCACTGCGCGTACACACACACACGCGCACACACACACACACACACACACCTCCCTTCCAGTTCC
AGCTGACGGTGACTTTTTGACTGGTAACCATAAAAGAATTCATTCAAAAGGGCAGTTGTTCCCAGCAGCCAGTGAGGTCCTTCCTGCATTCGTTGTCTTG
GAGAAGGAGGCACATAACAAAATAGAAACCAAAATGGGAAGGAAAAGAGAAAGAAAGTGAAATTCCTGGTCTTTGTTGTATGTATCCCCTTGCTTTTTAG
ATAAATTATCAGGGGTTTAAACACACACTCTCTCCCCTCCCTCCTCCCACTTCACACACACTAGATTGTTCTTGTCTGTTTGCAAACAGTCCAGAAGCAC
TGGGGTCTCATTAAATTAGATTCTAAGTGTCTTAAAGAATTAGATTTCAAGAAACCAAGAATCTGTGTCCTTTCCCTTCTCAGAGTGCCTGGAAGGGTGC
AGATTTCTCCAATCTGAATCTGACAATGTTATTTCTTATGTCAGATAATCCCAAGTAGATCTTATACCTTAAAAAAATAAAATCAGCTACACTACCTACC
TTACAGGGAAGGAAAAAGAACTAATTCCAATTATTAACTAACCCAAGATACAAACACAGGGGAAACTAGGGAATGTGCTTTGCAGCAATGTAAGATTATA
GGGGTTGGTTTTAACCATCCTTTGAATGTCCCTCTTTAAGCCTTTTTAAAGGGCAATTTTGCACGGTGAGTAGAATTAGGCTTCTCCGTGGTCAAGATTT
TGTTCACTCTATCAGTGCAAATTAAGTGGCACCGGCCCACTGGAAATTATAGTCTCCATTAGGAAGAAAAGAAATTACTTACCGAGCAGCAAATTCTCTA
AATTTGTAATTCAGATCTTTAGTGCAGAATGCCGGTCAGGATCCAGCCCTTTGCATAAATTGGTTTTCTGGGGAGTTTAACCCCTTAGCCTCTGTTGCCC
TTTTCTTGAATGGCATTCACGCAACCCTCTCCATCTCCTGGCCCTTTAACTGGCCCCGGCAGAAGTTGCAGTCCACAATTTCACAGAAGTAAGGATAATA
CATTATCACACATTACCGTTTATAAAGTGCTCTGGGTACAATCTGTAATTTAAAGCTGTATTTCCCTCACTGAACTTAAGTGCTTCCTTTGTTACATTTC
ATTACCGGGGGACTGGCATGCACCTGTATAGATTTTTTTTTTTTTTTTCATTTTTTGTTTTGTTTTGTTTTTCCAAATAAGCACTTCTTGACAAAATGGC
CCCCAGCTGCACTTCTGCTAATGACAGCCATGGAAGGGCTGGTTCCTTACGTGATCTACTCTCTCAGAAGCTTGCTTTTGTCAAAATGCAGCCACGTGGG
GGAGCTGCAGGACCTGGGGTTTTACAATGCCTAGCCCTTTTTCCCTGATTGCCTAACCGGGAAACTGGGCCAAAGCAGCCTGAGAGTGTATTTGTTGATT
AACTTCTAGAAAGCAGTCAAGTTTAATTAGGAGAACTAGAACGAGAAAGCTGAACTGGGCAAGTCAGTTGCCTTCTATGATTCTCAGTCCTCCAAATGAA
GATAAGGTTGGTTGGTTTGTGTTTCCATCCTTATCTGCTCTATCTTGGAAGAAGCTCAAGGAAGATTGTGTGATTGCAAAACGTTTCAGTTGGTGTCATT
GCAATGCATTTAAGTAATCCTTACTGGCCCATCCTGCTCCACAAAGCAGCCTTACCTCTCATCTTCTCCACTCCCAACTAAAGAGCAAGTGATTTAAACT
CTGAGTGTCCTCCCTCAACAATGGGCTAATAATAGAGCCTACTCGTGGAGTTGCTGCCAGCATGCAGTGAGATGGTGCATACCAAACATTTAGTGCAGTG
GCTGGCACATTGTAAGCACTTAGGAAAGGCAAGCTATTCTATCATGGGGAACTTTTAGCACCAAGTAACAGAAAACCCCTACTTGAATTGGCAGATAATC
GTAACAATAAGGGTATTTATCATTTCATATAATAGGTGTTGTATAACATCTTTGGCTTTGATTCTCTGAGACTCTCTTCCAGCTAACAACGATAATGAGG
AAATGTATCGTTTCATATAACTGGTGTTAAGTCTCATAGCCTGGCTCTGGTTCTCTGACACTCCATTCTGCCATGTTCCATGGGTTGACTTCATCCTAAA
ACTGTGCTGAATGATGGATGCAGTTCCAGGCAAGGGGATTATGGGAATACCATAATCAGTTTCAATGAATCATTTTGGTGAAATCATGTTGAGAAGGCAA
TACAATGATACAGTAGACAGTAAAGCCATTATTGGTATCAGTGGCTTTAAGGCTAACCATTCCCCAAACTAACTGTGTCCCTTCAATTCACACCCATGGG
ATTTGTGTAATGACGGCCCCCACCTTCCAAAGCAGTGCCACTAGAATGTGAACTTATCAAGATCTGAACCTTCTTGTCTTGCTCATTCATTGTTCTATTG
TCAGTGTCTAGCACAATGCCTGGACATGGTAGATACTCAACAAATATTGGTTGAATGGATAAATTGCTAAACAAATATCCTGTGGCCACACCCATTGTGG
ATTGCCTTATCCTCTAGTGTGAATTTGTTTTAGCTCTGAATGTCTGATCACCCTAACCAGACATCTACCCTTCCCAGGTAACCAGCCCAGTGGTTCTAAA
AGTACAGTCCCAGGACCAGCAGCCTCAGCACCACCTGAGAACATGTTAGGAATGCAAACTCTCAAGCCCCACTCCATACCCGCAGAATCAAAAACTCTTG
GGGTGGGACCCAGTAACCTGTGTTTTAGCAGCCTCTCAGGAGGTTCCAATGAAGGAGAAAATTTGAAAACCACTGGTCCAGGTAGAGCTACCACTTCACT
GAGCATCAACAGCATGTTTGGTCCAAAGACTGGCCCTGAAGATGCTTAGATAAAAAAAGCTAACATTGAACCTCACTATATGCCAGGTATTGTGTGTCAC
ATGACTTAACTTTGTTCACTGCTTATTTGGCAGCTGAGGAAACTAAGGCTCACAGAGAAAAAAACAACTTTGCCCAAGAACACACATGGCAGAGCCAAGG
TTGAAATCCAACCTAAGTACATAAAAGCTTGCTTTCATGTCTGTGGGAGAGACAGGCAGGCAAAAAGATAATAATAATACAATGTAAGAGAAGCAAATGC
AGGGTGTCATGGGACTCAGGGGAAGGCACTGGCGCAAGACCACAGTGGGTGGAGGTTGGCCACTTTGGGGAGGTGACAGTTGAGTTAACTCACACAGAAT
GAATAAGCGTTGGACAAAAGGGCTCCCAGAAGAACAGCAAGTGCAAGAGCCCAGGGTCCACAGAGAGAATGGCAGGTAGCTTAGCATGGCTGAAGTGAGA
CTGTGAGGAGAACGGATAAGAGGATGGAAGCTGGGGAGGTGGCACAGATGGATCTCAAGGACTTGGAGTAAAAAGTCCATAGTAACGAGTTTAAATTGTC
TCTTGACGGCAATGGGGAGCCATGGAAGATATTTGAGCTGGAGGGTAACATAGATTTATATTTTGGCTGCAGGTAGGATCTAATACAAAAGCAATTCAAC
TAATATTCACTAAGTGACCTGAGTTATCTGTGAGATATTTATTATAAGGTGTTAAACTTCTTTGAAGATTGTAAGAAAGAGATAAAACCTCTAAATGGTT
TTGACCCTTCAAAGGACATTTAAATGGTTATTTCTAAGTGTCATAAACATGGACTTGGGATTTGGCCTCATCATTCACCCAGATGATTCATACCCATGAT
CCCAGGCTGTGAGGTCTTGAGCAAATTACTTCTTCTCAGAGCCTTAGTTTGTTCCTCTGTGGAATGGGAATGCTAACAGCACTTACCTCGGAAAGTTGCT
GAGAATAAACGAGACCGTGCATTTAGAGCATGTAGGCACCGTGTCTGGCAGGTAATGAGTGCTCAGTCAGCTTATTGATGAGGATGCAGATGACCTCAGC
CTGAGATAGAACAGATGTGTTCTCTTTCATGGACTGAAGGCCACCCAGACTTTGCGACATCCTTAAACTTTGCCCCAGTTTCTCCTTATCCTCATAAGCA
GTTGGCTGGCTGGCTTATCTAGAAAAAAGAAAGGAGGGAGGAGAAAAAGCAAAGAAAAATGTTCATTTTCCCTGCTGTTCACCCCATTCTAAAGTAAGGC
AGAATTTCTGGAGAGGGGGCTCAGGTCCACCCTTGGCTATCCTTGGTTGTACAGTTGCATCTACTTATAACGGTGGCCAAGTCGCCATGGCAGTAGGTGC
TGTGGCTGCCCTGCCCCATCGTCTTCAGACTACCCGAGTTTACCTGCAGCACTGACAGCTTCCCACCCCAAAACTCCTCGTTCTTTGCTTTTCCTCTCGG
TGGCTTTCTCCAGGGGCTGTAGGAGCTTGTTTAGCCCACCTGCATGGCATTTTGAAAATACCTGAAATTTTATGCCCCGAAGGACAATTCTCAATCAGTG
AAAGATGGGAGAGGAAAGGTAAACACCTCAGCTGCCCCATCTCTCAGTGGGACAATGTTGAGGGGTCGTCTTTGTGTTCTCTCAAGGGTCCTCAGCAAGA
CCGAGCCACCCGTCACCCACAGCAGTAACATACTCATGAGCACGCCCCCCCACCCCACCTTTTGTGCTTCCTGGGATCACTTCCCAAATAAACTACTTGC
ACCTA
  • BCAS1.F2 (forward primer)
  • BCAS1.R1 (reverse primer)
  • Single exon skipping

Frame 0

GGSWTGAGHR*NPRARPGPRQGPLCRQGSHASP
T*DRGSRRRSSLQAQGLQLF*QILQAGQGTGKGA
R*QPTGSQEGRASRQGG*GSWLIRAVR*CPCRE
GHS*RQGKRRTRTWNCGLLCPWGPRRTGDCKGR
FPGSSYSRE**FHHEFL*NSGFT*QS*NKKGPRR
HGIESRKCL*WTSWSEDIRDPG*RHQEKAPG*P
QARTRL*KIL*A*GC*KVTHHFS*P*VRQSQLY
IPGDPRGWQEFQRMQPIGAHTVRDNP*TCEGRHQ
GEIRTHLSASGQTVLEKGGV*VTSRDYKVQGSR
ISLTNSGPQRRRCCT*THRSETQKRRKQTKNLS
DGVSQTNDIRLHRKDYHTARA*TNRSTTEG*RGL
LEGQEVSSRDEQAEEQQAGSQRTSPVHRAGHGG
HELTAEWGQAPKET*EAAAVPWGLL*RPGTKAD
VGCSSANRPSIHRTSWQIQVHREVCLLPCQEEVA
FPGMELLS*NGCHCAYTHTRAHTHTHTHLPSSS
S*R*LFDW*P*KNSFKRAVVPSSQ*GPSCIRCL
GEGGT*QNRNQNGKEKRKKVKFLVFVVCIPLLFR
*IIRGLNTHSLPSLLPLHTH*IVLVCLQTVQKH
WGLIKLDSKCLKELDFKKPRICVLSLLRVPGRV
QISPI*I*QCYFLCQIIPSRSYTLKK*NQLHYLP
YREGKRTNSNY*LTQDTNTGETRECALQQCKII
GVGFNHPLNVPL*AFLKGNFAR*VELGFSVVKI
LFTLSVQIKWHRPTGNYSLH*EEKKLLTEQQIL*
ICNSDL*CRMPVRIQPFA*IGFLGSLTP*PLLP
FS*MAFTQPSPSPGPLTGPGRSCSPQFHRSKDN
TLSHITVYKVLWVQSVI*SCISLTELKCFLCYIS
LPGDWHAPV*IFFFFFIFCFVLFFQISTS*QNG
PQLHFC**QPWKGWFLT*STLSEACFCQNAATW
GSCRTWGFTMPSPFSLIA*PGNWAKAA*ECIC*L
TSRKQSSLIRRTRTRKLNWASQLPSMILSPPNE
DKVGWFVFPSLSALSWKKLKEDCVIAKRFSWCH
CNAFK*SLLAHPAPQSSLTSHLLHSQLKSK*FKL
*VSSLNNGLIIEPTRGVAASMQ*DGAYQTFSAV
AGTL*ALRKGKLFYHGELLAPSNRKPLLELADN
RNNKGIYHFI**VLYNIFGFDSLRLSSS*QR**G
NVSFHITGVKSHSLALVL*HSILPCSMG*LHPK
TVLNDGCSSRQGDYGNTIISFNESFW*NHVEKA
IQ*YSRQ*SHYWYQWL*G*PFPKLTVSLQFTPMG
FV**RPPPSKAVPLECELIKI*TFLSCSFIVLL
SVSSTMPGHGRYSTNIG*MDKLLNKYPVATPIV
DCLIL*CEFVLALNV*SP*PDIYPSQVTSPVVLK
VQSQDQQPQHHLRTC*ECKLSSPTPYPQNQKLL
GWDPVTCVLAASQEVPMKEKI*KPLVQVELPLH
*ASTACLVQRLALKMLR*KKLTLNLTICQVLCVT
*LNFVHCLFGS*GN*GSQRKKQLCPRTHMAEPR
LKSNLST*KLAFMSVGETGRQKDNNNTM*EKQM
QGVMGLRGRHWRKTTVGGGWPLWGGDS*VNSHRM
NKRWTKGLPEEQQVQEPRVHRENGR*LSMAEVR
L*GERIRGWKLGRWHRWISRTWSKKSIVTSLNC
LLTAMGSHGRYLSWRVT*IYILAAGRI*YKSNST
NIH*VT*VICEIFIIRC*TSLKIVRKR*NL*MV
LTLQRTFKWLFLSVINMDLGFGLIIHPDDSYP*
SQAVRS*ANYFFSEP*FVPLWNGNANSTYLGKLL
RINETVHLEHVGTVSGR**VLSQLIDEDADDLS
LR*NRCVLFHGLKATQTLRHP*TLPQFLLILIS
SWLAGLSRKKKGGRRKSKEKCSFSLLFTPF*SKA
EFLERGLRSTLGYPWLYSCIYL*RWPSRHGSRC
CGCPAPSSSDYPSLPAALTASHPKTPRSLLFLS
VAFSRGCRSLFSPPAWHFENT*NFMPRRTILNQ*
KMGEER*TPQLPHLSVGQC*GVVFVFSQGSSAR
PSHPSPTAVTYS*ARPPTPPFVLPGITSQINYL
HL

Frame 1

VAAGPGQDTDKTPGHAPAQDKVLSAARDPTLLP
PETGGAGGEAPSKPKDSSFFDKFFKLDKGQEKV
PGDSQQEAKRAEHQDKVDEVPGLSGQSDDVPAGK
DIVDGKEKEGQELGTADCSVPGDPEGLETAKDD
SQAAAIAENNNSIMSFFKTLVSPNKAETKKDPE
DTASKAESVCDGQAGQKTSEIQARGTKKKHLDSP
RLGLAFRKFFRHKGAEKSPTTSADLKSDKANFT
SQETQGAGKNSKGCNPSGHTQSVTTPEPAKEGT
KEKSGPTSLPLGKLFWKKVVCESPVEIIKSKEVE
SALQTVDLNEGDAAPEPTEAKLKREESKPRTSL
MAFLRQMTSDSTEKTITPPEPEPTGAPQKGKEG
SSKDKKSAAEMNKQKSNKQEAKEPAQCTEQATVD
TNSLQNGDKLQKRPEKRQQSLGGFFKGLGPKRM
LDAQVQTDPVSIGPVGKSKYTGKCVFSHVKKKW
PFQEWSY*AEMAATARTHTHAHTHTHTHTSLPVP
ADGDFLTGNHKRIHSKGQLFPAASEVLPAFVVL
EKEAHNKIETKMGRKRERK*NSWSLLYVSPCFL
DKLSGV*THTLSPPSSHFTHTRLFLSVCKQSRST
GVSLN*ILSVLKN*ISRNQESVSFPFSECLEGC
RFLQSESDNVISYVR*SQVDLIP*KNKISYTTY
LTGKEKELIPIIN*PKIQTQGKLGNVLCSNVRL*
GLVLTIL*MSLFKPF*RAILHGE*N*ASPWSRF
CSLYQCKLSGTGPLEIIVSIRKKRNYLPSSKFS
KFVIQIFSAECRSGSSPLHKLVFWGV*PLSLCCP
FLEWHSRNPLHLLAL*LAPAEVAVHNFTEVRII
HYHTLPFIKCSGYNL*FKAVFPSLNLSASFVTF
HYRGTGMHLYRFFFFFSFFVLFCFSK*ALLDKMA
PSCTSANDSHGRAGSLRDLLSQKLAFVKMQPRG
GAAGPGVLQCLALFP*LPNRETGPKQPESVFVD
*LLESSQV*LGELERES*TGQVSCLL*FSVLQMK
IRLVGLCFHPYLLYLGRSSRKIV*LQNVSVGVI
AMHLSNPYWPILLHKAALPLIFSTPN*RASDLN
SECPPSTMG***SLLVELLPACSEMVHTKHLVQW
LAHCKHLGKASYSIMGNF*HQVTENPYLNWQII
VTIRVFIISYNRCCITSLALIL*DSLPANNDNE
EMYRFI*LVLSLIAWLWFSDTPFCHVPWVDFILK
LC*MMDAVPGKGIMGIP*SVSMNHFGEIMLRRQ
YNDTVDSKAIIGISGFKANHSPN*LCPFNSHPW
DLCNDGPHLPKQCH*NVNLSRSEPSCLAHSLFYC
QCLAQCLDMVDTQQILVEWINC*TNILWPHPLW
IALSSSVNLF*L*MSDHPNQTSTLPR*PAQWF*
KYSPRTSSLSTT*EHVRNANSQAPLHTRRIKNSW
GGTQ*PVF*QPLRRFQ*RRKFENHWSR*SYHFT
EHQQHVWSKDWP*RCLDKKS*H*TSLYARYCVS
HDLTLFTAYLAAEETKAHREKNNFAQEHTWQSQG
*NPT*VHKSLLSCLWERQAGKKIIIIQCKRSKC
RVSWDSGEGTGARPQWVEVGHFGEVTVELTHTE
*ISVGQKGSQKNSKCKSPGSTERMAGSLAWLK*D
CEENG*EDGSWGGGTDGSQGLGVKSP**RV*IV
S*RQWGAMEDI*AGG*HRFIFWLQVGSNTKAIQ
LIFTK*PELSVRYLL*GVKLL*RL*ERDKTSKWF
*PFKGHLNGYF*VS*TWTWDLASSFTQMIHTHD
PRL*GLEQITSSQSLSLFLCGMGMLTALTSESC
*E*TRPCI*SM*APCLAGNECSVSLLMRMQMTSA
*DRTDVFSFMD*RPPRLCDILKLCPSFSLSS*A
VGWLAYLEKRKEGGEKAKKNVHFPCCSPHSKVR
QNFWRGGSGPPLAILGCTVASTYNGGQVAMAVGA
VAALPHRLQTTRVYLQH*QLPTPKLLVLCFSSR
WLSPGAVGACLAHLHGILKIPEILCPEGQFSIS
ERWERKGKHLSCPISQWDNVEGSSLCSLKGPQQD
RATRHPQQ*HTHEHAPPPHLLCFLGSLPK*TTC
T

Frame 2

WQLDRGRTQIKPQGTPRPKTRSSLPPGIPRFSH
LRQGEQEEKLPPSPRTPAFLTNSSSWTRDRKRC
QVTANRKPRGQSIKTRWMRFLAYQGSPMMSLQG
RT*LTARKKKDKNLELRIALSLGTQKDWRLQRTI
PRQQL*QRIIIPS*VSLKLWFHLTKLKQKRTQK
TRHRKQKVSVMDKLVRRHPRSRLEAPRKSTWIA
PG*DSPLENSLGIRVLKSHPPLQLTLSQTKPTLH
PRRPKGLARIPKDATHRGTHSP*QPLNLRRKAP
RRNQDPPLCLWANCFGKRWCVSHQ*RL*SPRK*
NQPYKQWTSTKEMLHLNPQKRNSKEKKANQEPL*
WRFSDK*HQTPQKRLSHRQSLNQQEHHRRVKRA
PRRTRSQQPR*TSRRATSRKPKNQPSAQSRPRW
TRTHCRMGTSSKRDLRSGSSPLGASLKAWDQSGC
WMLKCKQTQYPSDQLANPSTQGSVSSPMSRRSG
LSRNGAIELKWLPLRVHTHTRTHTHTHTPPFQF
QLTVTF*LVTIKEFIQKGSCSQQPVRSFLHSLSW
RRRHITK*KPKWEGKEKESEIPGLCCMYPLAF*
INYQGFKHTLSPLPPPTSHTLDCSCLFANSPEA
LGSH*IRF*VS*RIRFQETKNLCPFPSQSAWKGA
DFSNLNLTMLFLMSDNPK*ILYLKKIKSATLPT
LQGRKKN*FQLLTNPRYKHRGN*GMCFAAM*DY
RGWF*PSFECPSLSLFKGQFCTVSRIRLLRGQDF
VHSISAN*VAPAHWKL*SPLGRKEITYRAANSL
NL*FRSLVQNAGQDPALCINWFSGEFNPLASVA
LFLNGIHATLSISWPFNWPRQKLQSTISQK*G*Y
IITHYRL*SALGTICNLKLYFPH*T*VLPLLHF
ITGGLACTCIDFFFFFHFLFCFVFPNKHFLTKW
PPAALLLMTAMEGLVPYVIYSLRSLLLSKCSHVG
ELQDLGFYNA*PFFPDCLTGKLGQSSLRVYLLI
NF*KAVKFN*EN*NEKAELGKSVAFYDSQSSK*
R*GWLVCVSILICSILEEAQGRLCDCKTFQLVSL
QCI*VILTGPSCSTKQPYLSSSPLPTKEQVI*T
LSVLPQQWANNRAYSWSCCQHAVRWCIPNI*CS
GWHIVST*ERQAILSWGTFSTK*QKTPT*IGR*S
*Q*GYLSFHIIGVV*HLWL*FSETLFQLTTIMR
KCIVSYNWC*VS*PGSGSLTLHSAMFHGLTSS*
NCAE*WMQFQARGLWEYHNQFQ*IILVKSC*EGN
TMIQ*TVKPLLVSVALRLTIPQTNCVPSIHTHG
ICVMTAPTFQSSATRM*TYQDLNLLVLLIHCSI
VSV*HNAWTW*ILNKYWLNG*IAKQISCGHTHCG
LPYPLV*ICFSSECLITLTRHLPFPGNQPSGSK
STVPGPAASAPPENMLGMQTLKPHSIPAESKTL
GVGPSNLCFSSLSGGSNEGENLKTTGPGRATTSL
SINSMFGPKTGPEDA*IKKANIEPHYMPGIVCH
MT*LCSLLIWQLRKLRLTEKKTTLPKNTHGRAK
VEIQPKYIKACFHVCGRDRQAKR***YNVREANA
GCHGTQGKALAQDHSGWRLATLGR*QLS*LTQN
E*ALDKRAPRRTASARAQGPQREWQVA*HG*SE
TVRRTDKRMEAGEVAQMDLKDLE*KVHSNEFKLS
LDGNGEPWKIFELEGNIDLYFGCR*DLIQKQFN
*YSLSDLSYL*DIYYKVLNFFEDCKKEIKPLNG
FDPSKDI*MVISKCHKHGLGIWPHHSPR*FIPMI
PGCEVLSKLLLLRALVCSSVEWEC*QHLPRKVA
ENKRDRAFRACRHRVWQVMSAQSAY**GCR*PQ
PEIEQMCSLSWTEGHPDFATSLNFAPVSPYPHKQ
LAGWLI*KKERREEKKQRKMFIFPAVHPILK*G
RISGEGAQVHPWLSLVVQLHLLITVAKSPWQ*V
LWLPCPIVFRLPEFTCSTDSFPPQNSSFFAFPLG
GFLQGL*ELV*PTCMAF*KYLKFYAPKDNSQSV
KDGRGKVNTSAAPSLSGTMLRGRLCVLSRVLSK
TEPPVTHSSNILMSTPPHPTFCASWDHFPNKLLA
P

Sequence BCAS1.aAug05

GTATTCAGCCAGCAGGCTCTGCACAAGGAAGCAAAGTGCAGGGAGCCAGGCTCCACCGACAGCCAGGCACTGGGCAGCACGCACTGGAGACCCAGGACCC
TGTGCAGGAGCAGCTCCGGGTGACACGAGGGGACTGAAGATACTCCCACAGGGGCTCAGCAGGAGCAATGGGTAACCAAATGAGTGTTCCCCAAAGAGTT
GAAGACCAAGAGAATGAACCAGAAGCAGAGACTTACCAGGACAACGCGTCTGCTCTGAACGGGGTTCCAGTGGTGGTGTCGACCCACACAGTTCAGCACT
TAGAGGAAGTCGACTTGGGAATAAGTGTCAAGACGGATAATGTGGCCACTTCTTCCCCCGAGACAACGGAGATAAGTGCTGTTGCGGATGCCAACGGAAA
GAATCTTGGGAAAGAGGCCAAACCCGAGGCACCAGCTGCTAAATCTCGTTTTTTCTTGATGCTCTCTCGGCCTGTACCAGGACGTACCGGAGACCAAGCC
GCAGATTCATCCCTTGGATCAGTGAAGCTTGATGTCAGCTCCAATAAAGCTCCAGCGAACAAAGACCCAAGTGAGAGCTGGACACTTCCGGTGGCAGCTG
GACCGGGGCAGGACACAGATAAAACCCCAGGGCACGCCCCGGCCCAAGACAAGGTCCTCTCTGCCGCCAGGGATCCCACGCTTCTCCCACCTGAGACAGG
GGGAGCAGGAGGAGAAGCTCCCTCCAAGCCCAAGGACTCCAGCTTTTTTGACAAATTCTTCAAGCTGGACAAGGGACAGGAAAAGGTGCCAGGTGACAGC
CAACAGGAAGCCAAGAGGGCAGAGCATCAAGACAAGGTGGATGAGGTTCCTGGCTTATCAGGGCAGTCCGATGATGTCCCTGCAGGGAAGGACATAGTTG
ACGGCAAGGAAAAAGAAGGACAAGAACTTGGAACTGCGGATTGCTCTGTCCCTGGGGACCCAGAAGGACTGGAGACTGCAAAGGACGATTCCCAGGCAGC
AGCTATAGCAGAGAATAATAATTCCATCATGAGTTTCTTTAAAACTCTGGTTTCACCTAACAAAGCTGAAACAAAAAAGGACCCAGAAGACACGGGTGCT
GAAAAGTCACCCACCACTTCAGCTGACCTTAAGTCAGACAAAGCCAACTTTACATCCCAGGAGACCCAAGGGGCTGGCAAGAATTCCAAAGGATGCAACC
CATCGGGGCACACACAGTCCGTGACAACCCCTGAACCTGCGAAGGAAGGCACCAAGGAGAAATCAGGACCCACCTCTCTGCCTCTGGGCAAACTGTTTTG
GAAAAAGTCAGTTAAAGAGGACTCAGTCCCCACAGGTGCGGAGGAGAATGTGGTGTGTGAGTCACCAGTAGAGATTATAAAGTCCAAGGAAGTAGAATCA
GCCTTACAAACAGTGGACCTCAACGAAGGAGATGCTGCACCTGAACCCACAGAAGCGAAACTCAAAAGAGAAGAAAGCAAACCAAGAACCTCTCTGATGG
CGTTTCTCAGACAAATGTCAGTGAAAGGGGATGGAGGGATCACCCACTCAGAAGAAATAAATGGGAAAGACTCCAGCTGCCAAACATCAGACTCCACAGA
AAAGACTATCACACCGCCAGAGCCTGAACCAACAGGAGCACCACAGAAGGGTAAAGAGGGCTCCTCGAAGGACAAGAAGTCAGCAGCCGAGATGAACAAG
CAGAAGAGCAACAAGCAGGAAGCCAAAGAACCAGCCCAGTGCACAGAGCAGGCCACGGTGGACACGAACTCACTGCAGAATGGGGACAAGCTCCAAAAGA
GACCTGAGAAGCGGCAGCAGTCCCTTGGGGGCTTCTTTAAAGGCCTGGGACCAAAGCGGATGTTGGATGCTCAAGTGCAAACAGACCCAGTATCCATCGG
ACCAGTTGGCAAATCCAAGTAAACAAATCAGCACGGTTCCCACCAGGTTCTCCTGCCACCAAGATGTGTTCTCCTTACTCCATCTCCTCCCCAAACACGC
TCCATGTATATATTCTTCTGATGGCCAGCAAATGAAATTCTGCCTAGAAATTAAGCCCGAGCTGTTGTATATTGAGGTGTATTATTTACGTCTCTGGTCC
AGTCTTTTCTGGCAAATAACAGTAAAGATGGTTTAGCAGGTCACCTAGTTGGGTCAGAAGAGTCGATGATCACCAAGCAGGAAAGGGAGGGAATAGAGGA
ATGTGTTCGGGTTAAGTGATGAAAATGGCAGTGGTGGCCGGGCGTGGTGGCTCTCGCCTGTAATCTCAGCACTTTGGGAGGCCGAGGCAGGTGGATCACC
TGAGGTCAGGAGTTCAAGACTAGCCTGGCCAACATCATGAAACCCCGTCTCTACTAAAAATACAAAAATTAGCCAGGCATGGTGGCACACACCTGTAGTC
CCAGCTACTCGGGAGCCCAACGCACGAGAACCGCTTGTACCCAGGAGGTGGAGGTTGCAGTGAGCCGAAGTTGCACCATTGCACTCCACCCTGGGCGACA
GAGCAAGATTCTATCAAAAAAAAAAAAAGGCAGTGGCAAGTAAGTTATAGAAGAGAAATGCTGCTAGAAGGAATTAAGCGTTGTAGTAAATGCGTGCTTA
TCCTCTAAGCTTGAAGAAGGGAGACGAAAATCCATTTGTTTAAATTCACATCTCAAGGAGGGAGAACCCGGGCTGTGTTGGGTGGTTGCCAATTTCCTAG
AACGGAATGTGTGGGGTATAGAAAAAGGAATGAATAAGCGTTGTTTTTCAAATAGGGTCCTTGTAAGTTATTGATGAGAGGGAAAAGATTGACTGGGGAG
GGCTTAAAATGATTTGGGAAAACAATTGCTTTTGAGGCTCAGTGACAACGGCAAAGATTACAACTTAAAAAAAAAAAATAAATAAAAAATAAAGGAAGTT
GCACGGTTATTTTGCAACACAAGGGGGCGGCAAGGTCCCCATTTTTATCCTGTAATACTGTATCCCTAACAAAGATTTGGTCTCTGCTATCTTACATTAT
TAATGTTTCTCAGATGGCTGAGGGGCTCGCTTCATCTGTTCCGTCTGACACTTATCTCAAGTGTGTCTGTCATTCCTAATGTTCTCAGGATGTGCTCTGA
TAAAACCCTCCCCATAACCTCAGTTAATAAAAATTTACAGAAGACTTCTCAAATACCTGAGTTGTTTTTAATACCTGTACAAAGGAGTAAATAGGACCCT
GAGTCTATTAAAATGTAATTCAAAGTAGCATATGATTGACTGACAGTCATGTAAACTGTATCTTTCTTTTTCTGATTTAATAAAAAATACATTTACTTCT
AAAGTATCTTTTAATTTTCAACTTCAAAGCAGTCCCACTCTATATCTGAGATGACTCTAACTTCTACATTATAAAATCCATACTCTTTCTCCATTATCTT
CTTTGGAAGATCATTCTCTCCCAGATCTTCCCGGATTTCCTTGTATATGGGCACACACACACACAAATGTGTGCAGTTGTATGTTTGCATGTACACACAC
ATGCATGTATAGAATAGTGCTGTCTTAGGCCAGGTTGCTTGGAGCAGAGCCTGAGATGGGGATTTGGACGCATAGGATATACTGAGAGTGGGCTCCGAGG
GAAAGGGAGTAAGAGAAAAAGAAGAGGGCAAGAAAAGAGTTGGGCAAGGGTTGGTTTCAACTGGAGCATGAATTGCACCACCCAGATAGTTTGACCTTGA
GTCAAGGGAGCCAGCCTTTGAACCCTGGTAGGTGAATTCTGGGCTGCCCTGGGAGGTGGGGGG
  • BCAS1.F2 (forward primer)
  • BCAS1.R1 (reverse primer)
  • Single exon skipping

Frame 0

VFSQQALHKEAKCREPGSTDSQALGSTHWRPRT
LCRSSSG*HEGTEDTPTGAQQEQWVTK*VFPKEL
KTKRMNQKQRLTRTTRLL*TGFQWWCRPTQFST
*RKSTWE*VSRRIMWPLLPPRQRR*VLLRMPTE
RILGKRPNPRHQLLNLVFS*CSLGLYQDVPETKP
QIHPLDQ*SLMSAPIKLQRTKTQVRAGHFRWQL
DRGRTQIKPQGTPRPKTRSSLPPGIPRFSHLRQ
GEQEEKLPPSPRTPAFLTNSSSWTRDRKRCQVTA
NRKPRGQSIKTRWMRFLAYQGSPMMSLQGRT*L
TARKKKDKNLELRIALSLGTQKDWRLQRTIPRQ
QL*QRIIIPS*VSLKLWFHLTKLKQKRTQKTRVL
KSHPPLQLTLSQTKPTLHPRRPKGLARIPKDAT
HRGTHSP*QPLNLRRKAPRRNQDPPLCLWANCF
GKSQLKRTQSPQVRRRMWCVSHQ*RL*SPRK*NQ
PYKQWTSTKEMLHLNPQKRNSKEKKANQEPL*W
RFSDKCQ*KGMEGSPTQKK*MGKTPAAKHQTPQ
KRLSHRQSLNQQEHHRRVKRAPRRTRSQQPR*TS
RRATSRKPKNQPSAQSRPRWTRTHCRMGTSSKR
DLRSGSSPLGASLKAWDQSGCWMLKCKQTQYPS
DQLANPSKQISTVPTRFSCHQDVFSLLHLLPKHA
PCIYSSDGQQMKFCLEIKPELLYIEVYYLRLWS
SLFWQITVKMV*QVT*LGQKSR*SPSRKGRE*R
NVFGLSDENGSGGRAWWLSPVISALWEAEAGGSP
EVRSSRLAWPTS*NPVSTKNTKISQAWWHTPVV
PATREPNAREPLVPRRWRLQ*AEVAPLHSTLGD
RARFYQKKKKAVASKL*KRNAARRN*AL**MRAY
PLSLKKGDENPFV*IHISRRENPGCVGWLPIS*
NGMCGV*KKE*ISVVFQIGSL*VIDEREKIDWG
GLKMIWENNCF*GSVTTAKITT*KKKINKK*RKL
HGYFATQGGGKVPIFIL*YCIPNKDLVSAILHY
*CFSDG*GARFICSV*HLSQVCLSFLMFSGCAL
IKPSP*PQLIKIYRRLLKYLSCF*YLYKGVNRTL
SLLKCNSK*HMID*QSCKLYLSFSDLIKNTFTS
KVSFNFQLQSSPTLYLR*L*LLHYKIHTLSPLS
SLEDHSLPDLPGFPCIWAHTHTNVCSCMFACTHT
CMYRIVLS*ARLLGAEPEMGIWTHRIY*EWAPR
ERE*EKKKRARKELGKGWFQLEHELHHPDSLTL
SQGSQPLNPGR*ILGCPGRWG

Frame 1

YSASRLCTRKQSAGSQAPPTARHWAARTGDPGP
CAGAAPGDTRGLKILPQGLSRSNG*PNECSPKS
*RPRE*TRSRDLPGQRVCSERGSSGGVDPHSSAL
RGSRLGNKCQDG*CGHFFPRDNGDKCCCGCQRK
ESWERGQTRGTSC*ISFFLDALSACTRTYRRPS
RRFIPWISEA*CQLQ*SSSEQRPK*ELDTSGGSW
TGAGHR*NPRARPGPRQGPLCRQGSHASPT*DR
GSRRRSSLQAQGLQLF*QILQAGQGTGKGAR*Q
PTGSQEGRASRQGG*GSWLIRAVR*CPCREGHS*
RQGKRRTRTWNCGLLCPWGPRRTGDCKGRFPGS
SYSRE**FHHEFL*NSGFT*QS*NKKGPRRHGC
*KVTHHFS*P*VRQSQLYIPGDPRGWQEFQRMQP
IGAHTVRDNP*TCEGRHQGEIRTHLSASGQTVL
EKVS*RGLSPHRCGGECGV*VTSRDYKVQGSRI
SLTNSGPQRRRCCT*THRSETQKRRKQTKNLSDG
VSQTNVSERGWRDHPLRRNKWERLQLPNIRLHR
KDYHTARA*TNRSTTEG*RGLLEGQEVSSRDEQ
AEEQQAGSQRTSPVHRAGHGGHELTAEWGQAPKE
T*EAAAVPWGLL*RPGTKADVGCSSANRPSIHR
TSWQIQVNKSARFPPGSPATKMCSPYSISSPNT
LHVYILLMASK*NSA*KLSPSCCILRCIIYVSGP
VFSGK*Q*RWFSRSPSWVRRVDDHQAGKGGNRG
MCSG*VMKMAVVAGRGGSRL*SQHFGRPRQVDH
LRSGVQD*PGQHHETPSLLKIQKLARHGGTHL*S
QLLGSPTHENRLYPGGGGCSEPKLHHCTPPWAT
EQDSIKKKKRQWQVSYRREMLLEGIKRCSKCVL
IL*A*RRETKIHLFKFTSQGGRTRAVLGGCQFPR
TECVGYRKRNE*ALFFK*GPCKLLMRGKRLTGE
GLK*FGKTIAFEAQ*QRQRLQLKKKK*IKNKGS
CTVILQHKGAARSPFLSCNTVSLTKIWSLLSYII
NVSQMAEGLASSVPSDTYLKCVCHS*CSQDVL*
*NPPHNLS**KFTEDFSNT*VVFNTCTKE*IGP
*VY*NVIQSSI*LTDSHVNCIFLFLI**KIHLLL
KYLLIFNFKAVPLYI*DDSNFYIIKSILFLHYL
LWKIILSQIFPDFLVYGHTHTQMCAVVCLHVHT
HACIE*CCLRPGCLEQSLRWGFGRIGYTESGLRG
KGSKRKRRGQEKSWARVGFNWSMNCTTQIV*P*
VKGASL*TLVGEFWAALGGGG

Frame 2

IQPAGSAQGSKVQGARLHRQPGTGQHALETQDP
VQEQLRVTRGD*RYSHRGSAGAMGNQMSVPQRV
EDQENEPEAETYQDNASALNGVPVVVSTHTVQH
LEEVDLGISVKTDNVATSSPETTEISAVADANGK
NLGKEAKPEAPAAKSRFFLMLSRPVPGRTGDQA
ADSSLGSVKLDVSSNKAPANKDPSESWTLPVAA
GPGQDTDKTPGHAPAQDKVLSAARDPTLLPPETG
GAGGEAPSKPKDSSFFDKFFKLDKGQEKVPGDS
QQEAKRAEHQDKVDEVPGLSGQSDDVPAGKDIV
DGKEKEGQELGTADCSVPGDPEGLETAKDDSQAA
AIAENNNSIMSFFKTLVSPNKAETKKDPEDTGA
EKSPTTSADLKSDKANFTSQETQGAGKNSKGCN
PSGHTQSVTTPEPAKEGTKEKSGPTSLPLGKLFW
KKSVKEDSVPTGAEENVVCESPVEIIKSKEVES
ALQTVDLNEGDAAPEPTEAKLKREESKPRTSLM
AFLRQMSVKGDGGITHSEEINGKDSSCQTSDSTE
KTITPPEPEPTGAPQKGKEGSSKDKKSAAEMNK
QKSNKQEAKEPAQCTEQATVDTNSLQNGDKLQK
RPEKRQQSLGGFFKGLGPKRMLDAQVQTDPVSIG
PVGKSK*TNQHGSHQVLLPPRCVLLTPSPPQTR
SMYIFF*WPANEILPRN*ARAVVY*GVLFTSLV
QSFLANNSKDGLAGHLVGSEESMITKQEREGIEE
CVRVK**KWQWWPGVVALACNLSTLGGRGRWIT
*GQEFKTSLANIMKPRLY*KYKN*PGMVAHTCS
PSYSGAQRTRTACTQEVEVAVSRSCTIALHPGRQ
SKILSKKKKGSGK*VIEEKCC*KELSVVVNACL
SSKLEEGRRKSICLNSHLKEGEPGLCWVVANFL
ERNVWGIEKGMNKRCFSNRVLVSY**EGKD*LGR
A*NDLGKQLLLRLSDNGKDYNLKKKNK*KIKEV
ARLFCNTRGRQGPHFYPVILYP*QRFGLCYLTL
LMFLRWLRGSLHLFRLTLISSVSVIPNVLRMCSD
KTLPITSVNKNLQKTSQIPELFLIPVQRSK*DP
ESIKM*FKVAYD*LTVM*TVSFFF*FNKKYIYF
*SIF*FSTSKQSHSISEMTLTSTL*NPYSFSIIF
FGRSFSPRSSRISLYMGTHTHKCVQLYVCMYTH
MHV*NSAVLGQVAWSRA*DGDLDA*DILRVGSE
GKGVREKEEGKKRVGQGLVSTGA*IAPPR*FDLE
SREPAFEPW*VNSGLPWEVG

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