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Sequences

Sequence APC.oAug05

TGACAAACTTGACTTTTGGAGATGTAGCCAACAAGGCTACGCTATGCTCTATGAAAGGCTGCATGAGAGCACTTGTGGCCCAACTAAAATCTGAAAGTGA AGACTTACAGCAGGTTATTGCGAGTGTTTTGAGGAATTTGTCTTGGCGAGCAGATGTAAATAGTAAAAAGACGTTGCGAGAAGTTGGAAGTGTGAAAGCA TTGATGGAATGTGCTTTAGAAGTTAAAAAGATGGCTCTACAAAAACAATTCCATGGTTTATTTGTATCTATCCTGCTTATTTAAATAATAAGAAGACCAT CGCAGAGGGAAGGCGAATCCCCATAAGTAAGGCTGTTGAAAATCCTACAGCTACAGAGATTCAAGATGTATGTTCAGCAGTTGGACTTAACGTATTTCTT GAGAAAAATAAAATGTACTCTAGAGAATGGAATCGTGATGTCCAATACAGAGGCAGAGTCCGGGTCCAGCTCAAACAGGAAGATGGGAGCCTCTGCCTTG TACAGTTCCCATCACGTAAGTCAGTAATGTTGTATGCAGCAGAAATGATACCTAAACTAAAAACAAGGACACAAAAAACAGGAGGTGCTGACCAAAGTCT TCAACAAGGAGAGGGAAGTAAAAAAGGGAAAGGAAAGAAAAAGAAGTAACCTAGTATCAGCATCAAGTATGTGGTACTACTGTAAGAGACATGAATGGAG ACTTCTAATTTGTATCGGAGGGAAACAGAAGCTTTTTGTTTGCATCATTTAACTGAACTGTGAACCCTTGTGCCTCTCATCTTTATCATCGGAGTTGACA GTGAAACAAATTTACATCAGAAGTTTGCATCTCGCGTATATGCCGTATAAAAGAATTTTTTTGTCTTTCAATGCAGTTTTTTGGAAGAAAATATTTTTAA ATGGACAATGGACTGTACAATAAGTTACTTGAAATAAGTTGTTTCAGATAAATTTCAATTAGATTTAAAATAAACATTTTGTCCACCTTTTAAGTTAATG AAATAAAATTTGAAACTGACTTTTGCAGCTTTTGCTTATATACTAATGCTAGGAGAGGAGGGATAATTAAGAATAAAATATGTAGTGAAAGTTTCCATAG TGTGAGGCTAAAACTAGAAGAAACTGTGGTAGGTCCTTTTGTGGGTGGGGGACAGGGGAGTCTGTAAAAAGCCAGTCTGTAGATGATATTTTAATATATT ATTGTAATCGAATCGTTCAGTTGTTTTTTGACATGGAAAGTCCTGGATTTTAAGCTTTAAATTTGCTTATTTTGTAGGTTTAAGAACATGATTTTCATGG GAGTTGTAAAATTAACTGTGCTTTAGCACCTTGAGGTACACTTTCCTTCAACAAATGAAATTGGATTGGTGCTCCAGAATTTCAGAGCGGTTTCTGAAAG TAGTGATTTTGAGCTATCCCAATTCCTGTTCTTCCTGAGGCCTGGTTTAGCTCTTCCTTGAATTCTGCAAGCTATCTCATATTTTCAGTAAATTCCATTT TTTTTAAGTTTGTTTGTTTGTTTGTTTTTGAGATGGAGTTTCACTCTTGTTGCCTAGGCTGGAGTGCAATGGCACGATATCGGCGTACCACAACCTCTGC ATCCCAGGTTCAAGAGATTCTCCTGCCTCAGCCTCCCTAGGAGCTGGGATTACAGGCATGTGCCACCACACCCGGCTAATTTTGTATTTTTAGTAGAGAC AGGGTTTTTCCATGTTGGTCAGGCTGGTCTCGAACTCCCGACCTCAGGTGATCTGCCCACCTCAGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCAC CGTGCCCGGCCTTCTTTACATTTTTATTGCTCACAGTCAAACATTCCTATTGATTGTAAAATAAGCAAACCTAGAGTTGTGGAGATTTATATGTATTTAT TTATTTGAATTGAGACAGGGTCTTAAACTCCCAGGCTCAAGTGATCCTCCTGCCTCAACCTCCCAAAGTGCTGGGATTACAGGCATGAGCTAGCTGGCCT GTTGACTTTTTTTTTTTAACTCTAACAATTTCTGTTTTATAGTGAACATAATAGTATAAGACTTAGTTTATATATTTGACTTACAAATAAATCCTGGGGA GAGGGGAGTATGTGTAAAACATAATTCAGAGCAGCCTTCTTTGAGAAAGTGGGTGTGTTTGGCTGTGTAACAAAACAATAAGGGGATGTGTTTGCTGTGT AACAAAACAATGAAGGATTTAAGCAGGATATTTTTGCCTCATATGTTAAATCCAAGTGAGGTTGCTGTGGTGGTTGGTTTAGCTCTCAGTGATGCCTTTT TTGTATTTTTCCGCTCTGCTGTCCATGACATATTTCTAACACCTTTATGATTATTGTTCCTGCTTGTAAAAGGGCTGATATTTACATGAGTGCAAGGCAG GAAGAAAAGGTAGCTGTGCCAGCCACTTCTGGCAAGCAGTTCTCCCACCTTAGCCTCCCAAGTAGCTGAGACCATAGGCATGAGATTTCTCAAAATTCCT CCCAGCAGGCTTTCACTTAGTTTCATTGTTGAGAACTGTGACAGGTCCATCTCTAGCTGCAAAGGAGGCTGAGAAAGTGAACACAGCAGTCCTCCTTATC CTTGGGGAATACATTCCAAGAGTGGATCCCTGAAACAGCAGATAGTACTGAACCCTATATATACTATGTTTTTGCCTATGTATATATACTTGATATGGTT TGGCTGCTACCCAACCCAAAATCTCATCTTGAATTATAATCCCCAAATCCCTATGTGTTAAGGGTGGGACCAGGTGGAGATAATTGGATCATGGGGGCAA TTTCCCCTGTGCTGTTCTTGAGATAATGAGTGACTCTCAGGAGATCTGTTGGTTTTATAAGTGCCTGGCGTTTCCCCTGCTTGCAGTCACTCCATCTTGC TGCCCTGTGAAGGTGCCTGCTTCTCCTTGCCTTCTGCCATGATTGTAAGTTTCCTGAGGCCTTCCCAGCAATGCGGAACTGTGAGTCAGTTAAACCTGTT TCCTTTATAATTAATCAGTTTTGGATATTTCTTCATAGCAGTGTGAGAATGGATTAATACATACCTATGATAAAGTTTATAAATTGGACACAGTAACAGA TTAACAATAAAATAGAACAATTATAATAATATACTATAATAAAATGTATGTGG

APC.F12 (forward primer)
APC.R11 (reverse primer)
Alternative acceptor

Frame 0

*QT*LLEM*PTRLRYAL*KAA*EHLWPN*NLKV KTYSRLLRVF*GICLGEQM*IVKRRCEKLEV*KH *WNVL*KLKRWLYKNNSMVYLYLSCLFK**EDH RRGKANPHK*GC*KSYSYRDSRCMFSSWT*RIS *EK*NVL*RMES*CPIQRQSPGPAQTGRWEPLPC TVPIT*VSNVVCSRNDT*TKNKDTKNRRC*PKS STRRGK*KRERKEKEVT*YQHQVCGTTVRDMNG DF*FVSEGNRSFLFASFN*TVNPCASHLYHRS*Q *NKFTSEVCISRICRIKEFFCLSMQFFGRKYF* MDNGLYNKLLEISCFR*ISIRFKINILSTF*VN EIKFETDFCSFCLYTNARRGGIIKNKICSESFHS VRLKLEETVVGPFVGGGQGSL*KASL*MIF*YI IVIESFSCFLTWKVLDFKL*ICLFCRFKNMIFM GVVKLTVL*HLEVHFPSTNEIGLVLQNFRAVSES SDFELSQFLFFLRPGLALP*ILQAISYFQ*IPF FLSLFVCLFLRWSFTLVA*AGVQWHDIGVPQPL HPRFKRFSCLSLPRSWDYRHVPPHPANFVFLVET GFFHVGQAGLELPTSGDLPTSASQSAGITGMSH RARPSLHFYCSQSNIPIDCKISKPRVVEIYMYL FI*IETGS*TPRLK*SSCLNLPKCWDYRHELAGL LTFFF*L*QFLFYSEHNSIRLSLYI*LTNKSWG EGSMCKT*FRAAFFEKVGVFGCVTKQ*GDVFAV *QNNEGFKQDIFASYVKSK*GCCGGWFSSQ*CLF CIFPLCCP*HISNTFMIIVPACKRADIYMSARQ EEKVAVPATSGKQFSHLSLPSS*DHRHEISQNS SQQAFT*FHC*EL*QVHL*LQRRLRK*TQQSSLS LGNTFQEWIPETADSTEPYIYYVFAYVYILDMV WLLPNPKSHLEL*SPNPYVLRVGPGGDNWIMGA ISPVLFLR**VTLRRSVGFISAWRFPCLQSLHLA AL*RCLLLLAFCHDCKFPEAFPAMRNCESVKPV SFIINQFWIFLHSSVRMD*YIPMIKFINWTQ*Q INNKIEQL**YTIIKCMW

Frame 1

DKLDFWRCSQQGYAMLYERLHESTCGPTKI*K* RLTAGYCECFEEFVLASRCK**KDVARSWKCES IDGMCFRS*KDGSTKTIPWFICIYPAYLNNKKTI AEGRRIPISKAVENPTATEIQDVCSAVGLNVFL EKNKMYSREWNRDVQYRGRVRVQLKQEDGSLCL VQFPSRKSVMLYAAEMIPKLKTRTQKTGGADQSL QQGEGSKKGKGKKKK*PSISIKYVVLL*ET*ME TSNLYRRETEAFCLHHLTEL*TLVPLIFIIGVD SETNLHQKFASRVYAV*KNFFVFQCSFLEENIFK WTMDCTISYLK*VVSDKFQLDLK*TFCPPFKLM K*NLKLTFAAFAYILMLGEEG*LRIKYVVKVSI V*G*N*KKLW*VLLWVGDRGVCKKPVCR*YFNIL L*SNRSVVF*HGKSWILSFKFAYFVGLRT*FSW EL*N*LCFSTLRYTFLQQMKLDWCSRISERFLK VVILSYPNSCSS*GLV*LFLEFCKLSHIFSKFHF F*VCLFVCF*DGVSLLLPRLECNGTISAYHNLC IPGSRDSPASASLGAGITGMCHHTRLILYF**R QGFSMLVRLVSNSRPQVICPPQPPKVLGLQA*AT VPGLLYIFIAHSQTFLLIVK*ANLELWRFICIY LFELRQGLKLPGSSDPPASTSQSAGITGMS*LA C*LFFFNSNNFCFIVNIIV*DLVYIFDLQINPGE RGVCVKHNSEQPSLRKWVCLAV*QNNKGMCLLC NKTMKDLSRIFLPHMLNPSEVAVVVGLALSDAF FVFFRSAVHDIFLTPL*LLFLLVKGLIFT*VQGR KKR*LCQPLLASSSPTLASQVAETIGMRFLKIP PSRLSLSFIVENCDRSISSCKGG*ESEHSSPPY PWGIHSKSGSLKQQIVLNPIYTMFLPMYIYLIWF GCYPTQNLILNYNPQIPMC*GWDQVEIIGSWGQ FPLCCS*DNE*LSGDLLVL*VPGVSPACSHSIL LPCEGACFSLPSAMIVSFLRPSQQCGTVSQLNLF PL*LISFGYFFIAV*EWINTYL**SL*IGHSNR LTIK*NNYNNIL**NVC

Frame 2

TNLTFGDVANKATLCSMKGCMRALVAQLKSESE DLQQVIASVLRNLSWRADVNSKKTLREVGSVKA LMECALEVKKMALQKQFHGLFVSILLI*IIRRP SQREGESP*VRLLKILQLQRFKMYVQQLDLTYFL RKIKCTLENGIVMSNTEAESGSSSNRKMGASAL YSSHHVSQ*CCMQQK*YLN*KQGHKKQEVLTKV FNKEREVKKGKERKRSNLVSASSMWYYCKRHEWR LLICIGGKQKLFVCII*LNCEPLCLSSLSSELT VKQIYIRSLHLAYMPYKRIFLSFNAVFWKKIFL NGQWTVQ*VT*NKLFQINFN*I*NKHFVHLLS** NKI*N*LLQLLLIY*C*ERRDN*E*NM**KFP* CEAKTRRNCGRSFCGWGTGESVKSQSVDDILIY YCNRIVQLFFDMESPGF*ALNLLIL*V*EHDFHG SCKINCALAP*GTLSFNK*NWIGAPEFQSGF*K **F*AIPIPVLPEAWFSSSLNSASYLIFSVNSI FFKFVCLFVFEMEFHSCCLGWSAMARYRRTTTSA SQVQEILLPQPP*ELGLQACATTPG*FCIFSRD RVFPCWSGWSRTPDLR*SAHLSLPKCWDYRHEP PCPAFFTFLLLTVKHSY*L*NKQT*SCGDLYVFI YLN*DRVLNSQAQVILLPQPPKVLGLQA*ASWP VDFFFLTLTISVL**T**YKT*FIYLTYK*ILG RGEYV*NIIQSSLL*ESGCVWLCNKTIRGCVCCV TKQ*RI*AGYFCLIC*IQVRLLWWLV*LSVMPF LYFSALLSMTYF*HLYDYCSCL*KG*YLHECKA GRKGSCASHFWQAVLPP*PPK*LRP*A*DFSKFL PAGFHLVSLLRTVTGPSLAAKEAEKVNTAVLLI LGEYIPRVDP*NSR*Y*TLYILCFCLCIYT*YG LAATQPKISS*IIIPKSLCVKGGTRWR*LDHGGN FPCAVLEIMSDSQEICWFYKCLAFPLLAVTPSC CPVKVPASPCLLP*L*VS*GLPSNAEL*VS*TC FLYN*SVLDISS*QCENGLIHTYDKVYKLDTVTD *Q*NRTIIIIYYNKMYV

Sequence APC.mAug05

GGGACTACAGGCACATGCCACCATGTCCGGCTACTTTGGTATTTTTTTGTAGCAATGAGATTTCTCCATGTTGTCCAGGCTTGTCTCAAACTCCTGAGCT GAAGTGATCTGCTGGGATTACAGGCTGTTGAAAATCCTACAGCTACAGAGATTCAAGATGTATGTTCAGCAGTTGGACTTAACGTATTTCTTGAGAAAAA TAAAATGTACTCTAGAGAATGGAATCGTGATGTCCAATACAGAGGCAGAGTCCGGGTCCAGCTCAAACAGGAAGATGGGAGCCTCTGCCTTGTACAGTTC CCATCACGTAAGTCAGTAATGTTGTATGCAGCAGAAATGATACCTAAACTAAAAACAAGGACACAAAAAACAGGAGGTGCTGACCAAAGTCTTCAACAAG GAGAGGGAAGTAAAAAAGGGAAAGGAAAGAAAAAGAAGTAACCTAGTATCAGCATCAAGTATGTGGTACTACTGTAAGAGACATGAATGGAGACTTCTAA TTTGTATCGGAGGGAAACAGAAGCTTTTTGTTTGCATCATTTAACTGAACTGTGAACCCTTGTGCCTCTCATCTTTATCATCGGAGTTGACAGTGAAACA AATTTACATCAGAAGTTTGCATCTCGCGTATATGCCGTATAAAAGAATTTTTTTGTCTTTCAATGCAGTTTTTTGGAAGAAAATATTTTTAAATGGACAA TGGACTGTACAATAAGTTACTTGAAATAAGTTGTTTCAGATAAATTTCAATTAGATTTAAAATAAACATTTTGTCCACCTTTTAAGTTAATGAAATAAAA TTTGAAACTGACTTTTGCAGCTTTTGCTTATATACTAATGCTAGGAGAGGAGGGATAATTAAGAATAAAATATGTAGTGAAAGTTTCCATAGTGTGAGGC TAAAACTAGAAGAAACTGTGGTAGGTCCTTTTGTGGGTGGGGGACAGGGGAGTCTGTAAAAAGCCAGTCTGTAGATGATATTTTAATATATTATTGTAAT CGAATCGTTCAGTTGTTTTTTGACATGGAAAGTCCTGGATTTTAAGCTTTAAATTTGCTTATTTTGTAGGTTTAAGAACATGATTTTCATGGGAGTTGTA AAATTAACTGTGCTTTAGCACCTTGAGGTACACTTTCCTTCAACAAATGAAATTGGATTGGTGCTCCAGAATTTCAGAGCGGTTTCTGAAAGTAGTGATT TTGAGCTATCCCAATTCCTGTTCTTCCTGAGGCCTGGTTTAGCTCTTCCTTGAATTCTGCAAGCTATCTCATATTTTCAGTAAATTCCATTTTTTTTAAG TTTGTTTGTTTGTTTGTTTTTGAGATGGAGTTTCACTCTTGTTGCCTAGGCTGGAGTGCAATGGCACGATATCGGCGTACCACAACCTCTGCATCCCAGG TTCAAGAGATTCTCCTGCCTCAGCCTCCCTAGGAGCTGGGATTACAGGCATGTGCCACCACACCCGGCTAATTTTGTATTTTTAGTAGAGACAGGGTTTT TCCATGTTGGTCAGGCTGGTCTCGAACTCCCGACCTCAGGTGATCTGCCCACCTCAGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACCGTGCCCG GCCTTCTTTACATTTTTATTGCTCACAGTCAAACATTCCTATTGATTGTAAAATAAGCAAACCTAGAGTTGTGGAGATTTATATGTATTTATTTATTTGA ATTGAGACAGGGTCTTAAACTCCCAGGCTCAAGTGATCCTCCTGCCTCAACCTCCCAAAGTGCTGGGATTACAGGCATGAGCTAGCTGGCCTGTTGACTT TTTTTTTTTAACTCTAACAATTTCTGTTTTATAGTGAACATAATAGTATAAGACTTAGTTTATATATTTGACTTACAAATAAATCCTGGGGAGAGGGGAG TATGTGTAAAACATAATTCAGAGCAGCCTTCTTTGAGAAAGTGGGTGTGTTTGGCTGTGTAACAAAACAATAAGGGGATGTGTTTGCTGTGTAACAAAAC AATGAAGGATTTAAGCAGGATATTTTTGCCTCATATGTTAAATCCAAGTGAGGTTGCTGTGGTGGTTGGTTTAGCTCTCAGTGATGCCTTTTTTGTATTT TTCCGCTCTGCTGTCCATGACATATTTCTAACACCTTTATGATTATTGTTCCTGCTTGTAAAAGGGCTGATATTTACATGAGTGCAAGGCAGGAAGAAAA GGTAGCTGTGCCAGCCACTTCTGGCAAGCAGTTCTCCCACCTTAGCCTCCCAAGTAGCTGAGACCATAGGCATGAGATTTCTCAAAATTCCTCCCAGCAG GCTTTCACTTAGTTTCATTGTTGAGAACTGTGACAGGTCCATCTCTAGCTGCAAAGGAGGCTGAGAAAGTGAACACAGCAGTCCTCCTTATCCTTGGGGA ATACATTCCAAGAGTGGATCCCTGAAACAGCAGATAGTACTGAACCCTATATATACTATGTTTTTGCCTATGTATATATACTTGATATGGTTTGGCTGCT ACCCAACCCAAAATCTCATCTTGAATTATAATCCCCAAATCCCTATGTGTTAAGGGTGGGACCAGGTGGAGATAATTGGATCATGGGGGCAATTTCCCCT GTGCTGTTCTTGAGATAATGAGTGACTCTCAGGAGATCTGTTGGTTTTATAAGTGCCTGGCGTTTCCCCTGCTTGCAGTCACTCCATCTTGCTGCCCTGT GAAGGTGCCTGCTTCTCCTTGCCTTCTGCCATGATTGTAAGTTTCCTGAGGCCTTCCCAGCAATGCGGAACTGTGAGTCAGTTAAACCTGTTTCCTTTAT AATTAATCAGTTTTGGATATTTCTTCATAGCAGTGTGAGAATGGATTAATACATACCTATGATAAAGTTTATAAATTGGACACAGTAACAGATTAACAAT AAAATAGAACAATTATAATAATATACTATAATAAAATGTATGTGG

APC.F12 (forward primer)
APC.R11 (reverse primer)
Alternative acceptor

Frame 0

GTTGTCHHVRLLWYFFVAMRFLHVVQACLKLLS *SDLLGLQAVENPTATEIQDVCSAVGLNVFLEKN KMYSREWNRDVQYRGRVRVQLKQEDGSLCLVQF PSRKSVMLYAAEMIPKLKTRTQKTGGADQSLQQ GEGSKKGKGKKKK*PSISIKYVVLL*ET*METSN LYRRETEAFCLHHLTEL*TLVPLIFIIGVDSET NLHQKFASRVYAV*KNFFVFQCSFLEENIFKWT MDCTISYLK*VVSDKFQLDLK*TFCPPFKLMK*N LKLTFAAFAYILMLGEEG*LRIKYVVKVSIV*G *N*KKLW*VLLWVGDRGVCKKPVCR*YFNILL* SNRSVVF*HGKSWILSFKFAYFVGLRT*FSWEL* N*LCFSTLRYTFLQQMKLDWCSRISERFLKVVI LSYPNSCSS*GLV*LFLEFCKLSHIFSKFHFF* VCLFVCF*DGVSLLLPRLECNGTISAYHNLCIPG SRDSPASASLGAGITGMCHHTRLILYF**RQGF SMLVRLVSNSRPQVICPPQPPKVLGLQA*ATVP GLLYIFIAHSQTFLLIVK*ANLELWRFICIYLFE LRQGLKLPGSSDPPASTSQSAGITGMS*LAC*L FFFNSNNFCFIVNIIV*DLVYIFDLQINPGERG VCVKHNSEQPSLRKWVCLAV*QNNKGMCLLCNKT MKDLSRIFLPHMLNPSEVAVVVGLALSDAFFVF FRSAVHDIFLTPL*LLFLLVKGLIFT*VQGRKK R*LCQPLLASSSPTLASQVAETIGMRFLKIPPSR LSLSFIVENCDRSISSCKGG*ESEHSSPPYPWG IHSKSGSLKQQIVLNPIYTMFLPMYIYLIWFGC YPTQNLILNYNPQIPMC*GWDQVEIIGSWGQFPL CCS*DNE*LSGDLLVL*VPGVSPACSHSILLPC EGACFSLPSAMIVSFLRPSQQCGTVSQLNLFPL *LISFGYFFIAV*EWINTYL**SL*IGHSNRLTI K*NNYNNIL**NVC

Frame 1

GLQAHATMSGYFGIFL*Q*DFSMLSRLVSNS*A EVICWDYRLLKILQLQRFKMYVQQLDLTYFLRK IKCTLENGIVMSNTEAESGSSSNRKMGASALYSS HHVSQ*CCMQQK*YLN*KQGHKKQEVLTKVFNK EREVKKGKERKRSNLVSASSMWYYCKRHEWRLL ICIGGKQKLFVCII*LNCEPLCLSSLSSELTVKQ IYIRSLHLAYMPYKRIFLSFNAVFWKKIFLNGQ WTVQ*VT*NKLFQINFN*I*NKHFVHLLS**NK I*N*LLQLLLIY*C*ERRDN*E*NM**KFP*CEA KTRRNCGRSFCGWGTGESVKSQSVDDILIYYCN RIVQLFFDMESPGF*ALNLLIL*V*EHDFHGSC KINCALAP*GTLSFNK*NWIGAPEFQSGF*K**F *AIPIPVLPEAWFSSSLNSASYLIFSVNSIFFK FVCLFVFEMEFHSCCLGWSAMARYRRTTTSASQ VQEILLPQPP*ELGLQACATTPG*FCIFSRDRVF PCWSGWSRTPDLR*SAHLSLPKCWDYRHEPPCP AFFTFLLLTVKHSY*L*NKQT*SCGDLYVFIYL N*DRVLNSQAQVILLPQPPKVLGLQA*ASWPVDF FFLTLTISVL**T**YKT*FIYLTYK*ILGRGE YV*NIIQSSLL*ESGCVWLCNKTIRGCVCCVTK Q*RI*AGYFCLIC*IQVRLLWWLV*LSVMPFLYF SALLSMTYF*HLYDYCSCL*KG*YLHECKAGRK GSCASHFWQAVLPP*PPK*LRP*A*DFSKFLPA GFHLVSLLRTVTGPSLAAKEAEKVNTAVLLILGE YIPRVDP*NSR*Y*TLYILCFCLCIYT*YGLAA TQPKISS*IIIPKSLCVKGGTRWR*LDHGGNFP CAVLEIMSDSQEICWFYKCLAFPLLAVTPSCCPV KVPASPCLLP*L*VS*GLPSNAEL*VS*TCFLY N*SVLDISS*QCENGLIHTYDKVYKLDTVTD*Q *NRTIIIIYYNKMYV

Frame 2

DYRHMPPCPATLVFFCSNEISPCCPGLSQTPEL K*SAGITGC*KSYSYRDSRCMFSSWT*RIS*EK *NVL*RMES*CPIQRQSPGPAQTGRWEPLPCTV PIT*VSNVVCSRNDT*TKNKDTKNRRC*PKSSTR RGK*KRERKEKEVT*YQHQVCGTTVRDMNGDF* FVSEGNRSFLFASFN*TVNPCASHLYHRS*Q*N KFTSEVCISRICRIKEFFCLSMQFFGRKYF*MDN GLYNKLLEISCFR*ISIRFKINILSTF*VNEIK FETDFCSFCLYTNARRGGIIKNKICSESFHSVR LKLEETVVGPFVGGGQGSL*KASL*MIF*YIIVI ESFSCFLTWKVLDFKL*ICLFCRFKNMIFMGVV KLTVL*HLEVHFPSTNEIGLVLQNFRAVSESSD FELSQFLFFLRPGLALP*ILQAISYFQ*IPFFLS LFVCLFLRWSFTLVA*AGVQWHDIGVPQPLHPR FKRFSCLSLPRSWDYRHVPPHPANFVFLVETGF FHVGQAGLELPTSGDLPTSASQSAGITGMSHRAR PSLHFYCSQSNIPIDCKISKPRVVEIYMYLFI* IETGS*TPRLK*SSCLNLPKCWDYRHELAGLLT FFF*L*QFLFYSEHNSIRLSLYI*LTNKSWGEGS MCKT*FRAAFFEKVGVFGCVTKQ*GDVFAV*QN NEGFKQDIFASYVKSK*GCCGGWFSSQ*CLFCI FPLCCP*HISNTFMIIVPACKRADIYMSARQEEK VAVPATSGKQFSHLSLPSS*DHRHEISQNSSQQ AFT*FHC*EL*QVHL*LQRRLRK*TQQSSLSLG NTFQEWIPETADSTEPYIYYVFAYVYILDMVWLL PNPKSHLEL*SPNPYVLRVGPGGDNWIMGAISP VLFLR**VTLRRSVGFISAWRFPCLQSLHLAAL *RCLLLLAFCHDCKFPEAFPAMRNCESVKPVSFI INQFWIFLHSSVRMD*YIPMIKFINWTQ*QINN KIEQL**YTIIKCMW

Sequence APC.jAug05

GGCGGAAAAGCGGGCTGTCTCGGAAACTCAGAGCCGGGTTCCTCCCGGGTTTCTGCCGGGTTTCTCCCTGCGGCTCCTGGGTTGTTGAGACTCTTGTGAA GATGGCTTGCGCTGCCGCGCGGTCCCCGGCCGACCAGGACAGGCTGTTGAAAATCCTACAGCTACAGAGATTCAAGATGTATGTTCAGCAGTTGGACTTA ACGTATTTCTTGAGAAAAATAAAATGTACTCTAGAGAATGGAATCGTGATGTCCAATACAGAGGCAGAGTCCGGGTCCAGCTCAAACAGGAAGATGGGAG CCTCTGCCTTGTACAGTTCCCATCACGTAAGTCAGTAATGTTGTATGCAGCAGAAATGATACCTAAACTAAAAACAAGGACACAAAAAACAGGAGGTGCT GACCAAAGTCTTCAACAAGGAGAGGGAAGTAAAAAAGGGAAAGGAAAGAAAAAGAAGTAACCTAGTATCAGCATCAAGTATGTGGTACTACTGTAAGAGA CATGAATGGAGACTTCTAATTTGTATCGGAGGGAAACAGAAGCTTTTTGTTTGCATCATTTAACTGAACTGTGAACCCTTGTGCCTCTCATCTTTATCAT CGGAGTTGACAGTGAAACAAATTTACATCAGAAGTTTGCATCTCGCGTATATGCCGTATAAAAGAATTTTTTTGTCTTTCAATGCAGTTTTTTGGAAGAA AATATTTTTAAATGGACAATGGACTGTACAATAAGTTACTTGAAATAAGTTGTTTCAGATAAATTTCAATTAGATTTAAAATAAACATTTTGTCCACCTT TTAAGTTAATGAAATAAAATTTGAAACTGACTTTTGCAGCTTTTGCTTATATACTAATGCTAGGAGAGGAGGGATAATTAAGAATAAAATATGTAGTGAA AGTTTCCATAGTGTGAGGCTAAAACTAGAAGAAACTGTGGTAGGTCCTTTTGTGGGTGGGGGACAGGGGAGTCTGTAAAAAGCCAGTCTGTAGATGATAT TTTAATATATTATTGTAATCGAATCGTTCAGTTGTTTTTTGACATGGAAAGTCCTGGATTTTAAGCTTTAAATTTGCTTATTTTGTAGGTTTAAGAACAT GATTTTCATGGGAGTTGTAAAATTAACTGTGCTTTAGCACCTTGAGGTACACTTTCCTTCAACAAATGAAATTGGATTGGTGCTCCAGAATTTCAGAGCG GTTTCTGAAAGTAGTGATTTTGAGCTATCCCAATTCCTGTTCTTCCTGAGGCCTGGTTTAGCTCTTCCTTGAATTCTGCAAGCTATCTCATATTTTCAGT AAATTCCATTTTTTTTAAGTTTGTTTGTTTGTTTGTTTTTGAGATGGAGTTTCACTCTTGTTGCCTAGGCTGGAGTGCAATGGCACGATATCGGCGTACC ACAACCTCTGCATCCCAGGTTCAAGAGATTCTCCTGCCTCAGCCTCCCTAGGAGCTGGGATTACAGGCATGTGCCACCACACCCGGCTAATTTTGTATTT TTAGTAGAGACAGGGTTTTTCCATGTTGGTCAGGCTGGTCTCGAACTCCCGACCTCAGGTGATCTGCCCACCTCAGCCTCCCAAAGTGCTGGGATTACAG GCATGAGCCACCGTGCCCGGCCTTCTTTACATTTTTATTGCTCACAGTCAAACATTCCTATTGATTGTAAAATAAGCAAACCTAGAGTTGTGGAGATTTA TATGTATTTATTTATTTGAATTGAGACAGGGTCTTAAACTCCCAGGCTCAAGTGATCCTCCTGCCTCAACCTCCCAAAGTGCTGGGATTACAGGCATGAG CTAGCTGGCCTGTTGACTTTTTTTTTTTAACTCTAACAATTTCTGTTTTATAGTGAACATAATAGTATAAGACTTAGTTTATATATTTGACTTACAAATA AATCCTGGGGAGAGGGGAGTATGTGTAAAACATAATTCAGAGCAGCCTTCTTTGAGAAAGTGGGTGTGTTTGGCTGTGTAACAAAACAATAAGGGGATGT GTTTGCTGTGTAACAAAACAATGAAGGATTTAAGCAGGATATTTTTGCCTCATATGTTAAATCCAAGTGAGGTTGCTGTGGTGGTTGGTTTAGCTCTCAG TGATGCCTTTTTTGTATTTTTCCGCTCTGCTGTCCATGACATATTTCTAACACCTTTATGATTATTGTTCCTGCTTGTAAAAGGGCTGATATTTACATGA GTGCAAGGCAGGAAGAAAAGGTAGCTGTGCCAGCCACTTCTGGCAAGCAGTTCTCCCACCTTAGCCTCCCAAGTAGCTGAGACCATAGGCATGAGATTTC TCAAAATTCCTCCCAGCAGGCTTTCACTTAGTTTCATTGTTGAGAACTGTGACAGGTCCATCTCTAGCTGCAAAGGAGGCTGAGAAAGTGAACACAGCAG TCCTCCTTATCCTTGGGGAATACATTCCAAGAGTGGATCCCTGAAACAGCAGATAGTACTGAACCCTATATATACTATGTTTTTGCCTATGTATATATAC TTGATATGGTTTGGCTGCTACCCAACCCAAAATCTCATCTTGAATTATAATCCCCAAATCCCTATGTGTTAAGGGTGGGACCAGGTGGAGATAATTGGAT CATGGGGGCAATTTCCCCTGTGCTGTTCTTGAGATAATGAGTGACTCTCAGGAGATCTGTTGGTTTTATAAGTGCCTGGCGTTTCCCCTGCTTGCAGTCA CTCCATCTTGCTGCCCTGTGAAGGTGCCTGCTTCTCCTTGCCTTCTGCCATGATTGTAAGTTTCCTGAGGCCTTCCCAGCAATGCGGAACTGTGAGTCAG TTAAACCTGTTTCCTTTATAATTAATCAGTTTTGGATATTTCTTCATAGCAGTGTGAGAATGGATTAATACATACCTATGATAAAGTTTATAAATTGGAC ACAGTAACAGATTAACAATAAAATAGAACAATTATAATAATATACTATAATAAAATGTATGTGG

APC.F12 (forward primer)
APC.R11 (reverse primer)
Alternative acceptor

Frame 0

GGKAGCLGNSEPGSSRVSAGFLPAAPGLLRLL* RWLALPRGPRPTRTGC*KSYSYRDSRCMFSSWT* RIS*EK*NVL*RMES*CPIQRQSPGPAQTGRWE PLPCTVPIT*VSNVVCSRNDT*TKNKDTKNRRC *PKSSTRRGK*KRERKEKEVT*YQHQVCGTTVRD MNGDF*FVSEGNRSFLFASFN*TVNPCASHLYH RS*Q*NKFTSEVCISRICRIKEFFCLSMQFFGR KYF*MDNGLYNKLLEISCFR*ISIRFKINILSTF *VNEIKFETDFCSFCLYTNARRGGIIKNKICSE SFHSVRLKLEETVVGPFVGGGQGSL*KASL*MI F*YIIVIESFSCFLTWKVLDFKL*ICLFCRFKNM IFMGVVKLTVL*HLEVHFPSTNEIGLVLQNFRA VSESSDFELSQFLFFLRPGLALP*ILQAISYFQ *IPFFLSLFVCLFLRWSFTLVA*AGVQWHDIGVP QPLHPRFKRFSCLSLPRSWDYRHVPPHPANFVF LVETGFFHVGQAGLELPTSGDLPTSASQSAGIT GMSHRARPSLHFYCSQSNIPIDCKISKPRVVEIY MYLFI*IETGS*TPRLK*SSCLNLPKCWDYRHE LAGLLTFFF*L*QFLFYSEHNSIRLSLYI*LTN KSWGEGSMCKT*FRAAFFEKVGVFGCVTKQ*GDV FAV*QNNEGFKQDIFASYVKSK*GCCGGWFSSQ *CLFCIFPLCCP*HISNTFMIIVPACKRADIYM SARQEEKVAVPATSGKQFSHLSLPSS*DHRHEIS QNSSQQAFT*FHC*EL*QVHL*LQRRLRK*TQQ SSLSLGNTFQEWIPETADSTEPYIYYVFAYVYI LDMVWLLPNPKSHLEL*SPNPYVLRVGPGGDNWI MGAISPVLFLR**VTLRRSVGFISAWRFPCLQS LHLAAL*RCLLLLAFCHDCKFPEAFPAMRNCES VKPVSFIINQFWIFLHSSVRMD*YIPMIKFINWT Q*QINNKIEQL**YTIIKCMW

Frame 1

AEKRAVSETQSRVPPGFLPGFSLRLLGC*DSCE DGLRCRAVPGRPGQAVENPTATEIQDVCSAVGL NVFLEKNKMYSREWNRDVQYRGRVRVQLKQEDGS LCLVQFPSRKSVMLYAAEMIPKLKTRTQKTGGA DQSLQQGEGSKKGKGKKKK*PSISIKYVVLL*E T*METSNLYRRETEAFCLHHLTEL*TLVPLIFII GVDSETNLHQKFASRVYAV*KNFFVFQCSFLEE NIFKWTMDCTISYLK*VVSDKFQLDLK*TFCPP FKLMK*NLKLTFAAFAYILMLGEEG*LRIKYVVK VSIV*G*N*KKLW*VLLWVGDRGVCKKPVCR*Y FNILL*SNRSVVF*HGKSWILSFKFAYFVGLRT *FSWEL*N*LCFSTLRYTFLQQMKLDWCSRISER FLKVVILSYPNSCSS*GLV*LFLEFCKLSHIFS KFHFF*VCLFVCF*DGVSLLLPRLECNGTISAY HNLCIPGSRDSPASASLGAGITGMCHHTRLILYF **RQGFSMLVRLVSNSRPQVICPPQPPKVLGLQ A*ATVPGLLYIFIAHSQTFLLIVK*ANLELWRF ICIYLFELRQGLKLPGSSDPPASTSQSAGITGMS *LAC*LFFFNSNNFCFIVNIIV*DLVYIFDLQI NPGERGVCVKHNSEQPSLRKWVCLAV*QNNKGM CLLCNKTMKDLSRIFLPHMLNPSEVAVVVGLALS DAFFVFFRSAVHDIFLTPL*LLFLLVKGLIFT* VQGRKKR*LCQPLLASSSPTLASQVAETIGMRF LKIPPSRLSLSFIVENCDRSISSCKGG*ESEHSS PPYPWGIHSKSGSLKQQIVLNPIYTMFLPMYIY LIWFGCYPTQNLILNYNPQIPMC*GWDQVEIIG SWGQFPLCCS*DNE*LSGDLLVL*VPGVSPACSH SILLPCEGACFSLPSAMIVSFLRPSQQCGTVSQ LNLFPL*LISFGYFFIAV*EWINTYL**SL*IG HSNRLTIK*NNYNNIL**NVC

Frame 2

RKSGLSRKLRAGFLPGFCRVSPCGSWVVETLVK MACAAARSPADQDRLLKILQLQRFKMYVQQLDL TYFLRKIKCTLENGIVMSNTEAESGSSSNRKMG ASALYSSHHVSQ*CCMQQK*YLN*KQGHKKQEVL TKVFNKEREVKKGKERKRSNLVSASSMWYYCKR HEWRLLICIGGKQKLFVCII*LNCEPLCLSSLS SELTVKQIYIRSLHLAYMPYKRIFLSFNAVFWKK IFLNGQWTVQ*VT*NKLFQINFN*I*NKHFVHL LS**NKI*N*LLQLLLIY*C*ERRDN*E*NM** KFP*CEAKTRRNCGRSFCGWGTGESVKSQSVDDI LIYYCNRIVQLFFDMESPGF*ALNLLIL*V*EH DFHGSCKINCALAP*GTLSFNK*NWIGAPEFQS GF*K**F*AIPIPVLPEAWFSSSLNSASYLIFSV NSIFFKFVCLFVFEMEFHSCCLGWSAMARYRRT TTSASQVQEILLPQPP*ELGLQACATTPG*FCI FSRDRVFPCWSGWSRTPDLR*SAHLSLPKCWDYR HEPPCPAFFTFLLLTVKHSY*L*NKQT*SCGDL YVFIYLN*DRVLNSQAQVILLPQPPKVLGLQA* ASWPVDFFFLTLTISVL**T**YKT*FIYLTYK* ILGRGEYV*NIIQSSLL*ESGCVWLCNKTIRGC VCCVTKQ*RI*AGYFCLIC*IQVRLLWWLV*LS VMPFLYFSALLSMTYF*HLYDYCSCL*KG*YLHE CKAGRKGSCASHFWQAVLPP*PPK*LRP*A*DF SKFLPAGFHLVSLLRTVTGPSLAAKEAEKVNTA VLLILGEYIPRVDP*NSR*Y*TLYILCFCLCIYT *YGLAATQPKISS*IIIPKSLCVKGGTRWR*LD HGGNFPCAVLEIMSDSQEICWFYKCLAFPLLAV TPSCCPVKVPASPCLLP*L*VS*GLPSNAEL*VS *TCFLYN*SVLDISS*QCENGLIHTYDKVYKLD TVTD*Q*NRTIIIIYYNKMYV

Sequence APC.gAug05

GCCACGCAGAAAAAGGAGGGGTTGAAAAGGGAGTACCCATCTGATATCTAGGCAGCATGAATTGGCCTTTGGTTCCCTGCCACCCGACCCTTTTCTCCTG CCTCTTACTGCTCTAGTGATACCACTTATGCTATTGTCTATGGCAGGTTTATTTGTATCTATCCTGCTTATTTAAATAATAAGAAGACCATCGCAGAGGG AAGGCGAATCCCCATAAGTAAGGCTGTTGAAAATCCTACAGCTACAGAGATTCAAGATGTATGTTCAGCAGTTGGACTTAACGTATTTCTTGAGAAAAAT AAAATGTACTCTAGAGAATGGAATCGTGATGTCCAATACAGAGGCAGAGTCCGGGTCCAGCTCAAACAGGAAGATGGGAGCCTCTGCCTTGTACAGTTCC CATCACGTAAGTCAGTAATGTTGTATGCAGCAGAAATGATACCTAAACTAAAAACAAGGACACAAAAAACAGGAGGTGCTGACCAAAGTCTTCAACAAGG AGAGGGAAGTAAAAAAGGGAAAGGAAAGAAAAAGAAGTAACCTAGTATCAGCATCAAGTATGTGGTACTACTGTAAGAGACATGAATGGAGACTTCTAAT TTGTATCGGAGGGAAACAGAAGCTTTTTGTTTGCATCATTTAACTGAACTGTGAACCCTTGTGCCTCTCATCTTTATCATCGGAGTTGACAGTGAAACAA ATTTACATCAGAAGTTTGCATCTCGCGTATATGCCGTATAAAAGAATTTTTTTGTCTTTCAATGCAGTTTTTTGGAAGAAAATATTTTTAAATGGACAAT GGACTGTACAATAAGTTACTTGAAATAAGTTGTTTCAGATAAATTTCAATTAGATTTAAAATAAACATTTTGTCCACCTTTTAAGTTAATGAAATAAAAT TTGAAACTGACTTTTGCAGCTTTTGCTTATATACTAATGCTAGGAGAGGAGGGATAATTAAGAATAAAATATGTAGTGAAAGTTTCCATAGTGTGAGGCT AAAACTAGAAGAAACTGTGGTAGGTCCTTTTGTGGGTGGGGGACAGGGGAGTCTGTAAAAAGCCAGTCTGTAGATGATATTTTAATATATTATTGTAATC GAATCGTTCAGTTGTTTTTTGACATGGAAAGTCCTGGATTTTAAGCTTTAAATTTGCTTATTTTGTAGGTTTAAGAACATGATTTTCATGGGAGTTGTAA AATTAACTGTGCTTTAGCACCTTGAGGTACACTTTCCTTCAACAAATGAAATTGGATTGGTGCTCCAGAATTTCAGAGCGGTTTCTGAAAGTAGTGATTT TGAGCTATCCCAATTCCTGTTCTTCCTGAGGCCTGGTTTAGCTCTTCCTTGAATTCTGCAAGCTATCTCATATTTTCAGTAAATTCCATTTTTTTTAAGT TTGTTTGTTTGTTTGTTTTTGAGATGGAGTTTCACTCTTGTTGCCTAGGCTGGAGTGCAATGGCACGATATCGGCGTACCACAACCTCTGCATCCCAGGT TCAAGAGATTCTCCTGCCTCAGCCTCCCTAGGAGCTGGGATTACAGGCATGTGCCACCACACCCGGCTAATTTTGTATTTTTAGTAGAGACAGGGTTTTT CCATGTTGGTCAGGCTGGTCTCGAACTCCCGACCTCAGGTGATCTGCCCACCTCAGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACCGTGCCCGG CCTTCTTTACATTTTTATTGCTCACAGTCAAACATTCCTATTGATTGTAAAATAAGCAAACCTAGAGTTGTGGAGATTTATATGTATTTATTTATTTGAA TTGAGACAGGGTCTTAAACTCCCAGGCTCAAGTGATCCTCCTGCCTCAACCTCCCAAAGTGCTGGGATTACAGGCATGAGCTAGCTGGCCTGTTGACTTT TTTTTTTTAACTCTAACAATTTCTGTTTTATAGTGAACATAATAGTATAAGACTTAGTTTATATATTTGACTTACAAATAAATCCTGGGGAGAGGGGAGT ATGTGTAAAACATAATTCAGAGCAGCCTTCTTTGAGAAAGTGGGTGTGTTTGGCTGTGTAACAAAACAATAAGGGGATGTGTTTGCTGTGTAACAAAACA ATGAAGGATTTAAGCAGGATATTTTTGCCTCATATGTTAAATCCAAGTGAGGTTGCTGTGGTGGTTGGTTTAGCTCTCAGTGATGCCTTTTTTGTATTTT TCCGCTCTGCTGTCCATGACATATTTCTAACACCTTTATGATTATTGTTCCTGCTTGTAAAAGGGCTGATATTTACATGAGTGCAAGGCAGGAAGAAAAG GTAGCTGTGCCAGCCACTTCTGGCAAGCAGTTCTCCCACCTTAGCCTCCCAAGTAGCTGAGACCATAGGCATGAGATTTCTCAAAATTCCTCCCAGCAGG CTTTCACTTAGTTTCATTGTTGAGAACTGTGACAGGTCCATCTCTAGCTGCAAAGGAGGCTGAGAAAGTGAACACAGCAGTCCTCCTTATCCTTGGGGAA TACATTCCAAGAGTGGATCCCTGAAACAGCAGATAGTACTGAACCCTATATATACTATGTTTTTGCCTATGTATATATACTTGATATGGTTTGGCTGCTA CCCAACCCAAAATCTCATCTTGAATTATAATCCCCAAATCCCTATGTGTTAAGGGTGGGACCAGGTGGAGATAATTGGATCATGGGGGCAATTTCCCCTG TGCTGTTCTTGAGATAATGAGTGACTCTCAGGAGATCTGTTGGTTTTATAAGTGCCTGGCGTTTCCCCTGCTTGCAGTCACTCCATCTTGCTGCCCTGTG AAGGTGCCTGCTTCTCCTTGCCTTCTGCCATGATTGTAAGTTTCCTGAGGCCTTCCCAGCAATGCGGAACTGTGAGTCAGTTAAACCTGTTTCCTTTATA ATTAATCAGTTTTGGATATTTCTTCATAGCAGTGTGAGAATGGATTAATACATACCTATGATAAAGTTTATAAATTGGACACAGTAACAGATTAACAATA AAATAGAACAATTATAATAATATACTATAATAAAATGTATGTGG

APC.F12 (forward primer)
APC.R11 (reverse primer)
Alternative acceptor

Frame 0

ATQKKEGLKREYPSDI*AA*IGLWFPATRPFSP ASYCSSDTTYAIVYGRFICIYPAYLNNKKTIAEG RRIPISKAVENPTATEIQDVCSAVGLNVFLEKN KMYSREWNRDVQYRGRVRVQLKQEDGSLCLVQF PSRKSVMLYAAEMIPKLKTRTQKTGGADQSLQQG EGSKKGKGKKKK*PSISIKYVVLL*ET*METSN LYRRETEAFCLHHLTEL*TLVPLIFIIGVDSET NLHQKFASRVYAV*KNFFVFQCSFLEENIFKWTM DCTISYLK*VVSDKFQLDLK*TFCPPFKLMK*N LKLTFAAFAYILMLGEEG*LRIKYVVKVSIV*G *N*KKLW*VLLWVGDRGVCKKPVCR*YFNILL*S NRSVVF*HGKSWILSFKFAYFVGLRT*FSWEL* N*LCFSTLRYTFLQQMKLDWCSRISERFLKVVI LSYPNSCSS*GLV*LFLEFCKLSHIFSKFHFF*V CLFVCF*DGVSLLLPRLECNGTISAYHNLCIPG SRDSPASASLGAGITGMCHHTRLILYF**RQGF SMLVRLVSNSRPQVICPPQPPKVLGLQA*ATVPG LLYIFIAHSQTFLLIVK*ANLELWRFICIYLFE LRQGLKLPGSSDPPASTSQSAGITGMS*LAC*L FFFNSNNFCFIVNIIV*DLVYIFDLQINPGERGV CVKHNSEQPSLRKWVCLAV*QNNKGMCLLCNKT MKDLSRIFLPHMLNPSEVAVVVGLALSDAFFVF FRSAVHDIFLTPL*LLFLLVKGLIFT*VQGRKKR *LCQPLLASSSPTLASQVAETIGMRFLKIPPSR LSLSFIVENCDRSISSCKGG*ESEHSSPPYPWG IHSKSGSLKQQIVLNPIYTMFLPMYIYLIWFGCY PTQNLILNYNPQIPMC*GWDQVEIIGSWGQFPL CCS*DNE*LSGDLLVL*VPGVSPACSHSILLPC EGACFSLPSAMIVSFLRPSQQCGTVSQLNLFPL* LISFGYFFIAV*EWINTYL**SL*IGHSNRLTI K*NNYNNIL**NVC

Frame 1

PRRKRRG*KGSTHLISRQHELAFGSLPPDPFLL PLTALVIPLMLLSMAGLFVSILLI*IIRRPSQR EGESP*VRLLKILQLQRFKMYVQQLDLTYFLRKI KCTLENGIVMSNTEAESGSSSNRKMGASALYSS HHVSQ*CCMQQK*YLN*KQGHKKQEVLTKVFNK EREVKKGKERKRSNLVSASSMWYYCKRHEWRLLI CIGGKQKLFVCII*LNCEPLCLSSLSSELTVKQ IYIRSLHLAYMPYKRIFLSFNAVFWKKIFLNGQ WTVQ*VT*NKLFQINFN*I*NKHFVHLLS**NKI *N*LLQLLLIY*C*ERRDN*E*NM**KFP*CEA KTRRNCGRSFCGWGTGESVKSQSVDDILIYYCN RIVQLFFDMESPGF*ALNLLIL*V*EHDFHGSCK INCALAP*GTLSFNK*NWIGAPEFQSGF*K**F *AIPIPVLPEAWFSSSLNSASYLIFSVNSIFFK FVCLFVFEMEFHSCCLGWSAMARYRRTTTSASQV QEILLPQPP*ELGLQACATTPG*FCIFSRDRVF PCWSGWSRTPDLR*SAHLSLPKCWDYRHEPPCP AFFTFLLLTVKHSY*L*NKQT*SCGDLYVFIYLN *DRVLNSQAQVILLPQPPKVLGLQA*ASWPVDF FFLTLTISVL**T**YKT*FIYLTYK*ILGRGE YV*NIIQSSLL*ESGCVWLCNKTIRGCVCCVTKQ *RI*AGYFCLIC*IQVRLLWWLV*LSVMPFLYF SALLSMTYF*HLYDYCSCL*KG*YLHECKAGRK GSCASHFWQAVLPP*PPK*LRP*A*DFSKFLPAG FHLVSLLRTVTGPSLAAKEAEKVNTAVLLILGE YIPRVDP*NSR*Y*TLYILCFCLCIYT*YGLAA TQPKISS*IIIPKSLCVKGGTRWR*LDHGGNFPC AVLEIMSDSQEICWFYKCLAFPLLAVTPSCCPV KVPASPCLLP*L*VS*GLPSNAEL*VS*TCFLY N*SVLDISS*QCENGLIHTYDKVYKLDTVTD*Q* NRTIIIIYYNKMYV

Frame 2

HAEKGGVEKGVPI*YLGSMNWPLVPCHPTLFSC LLLL**YHLCYCLWQVYLYLSCLFK**EDHRRG KANPHK*GC*KSYSYRDSRCMFSSWT*RIS*EK *NVL*RMES*CPIQRQSPGPAQTGRWEPLPCTVP IT*VSNVVCSRNDT*TKNKDTKNRRC*PKSSTR RGK*KRERKEKEVT*YQHQVCGTTVRDMNGDF* FVSEGNRSFLFASFN*TVNPCASHLYHRS*Q*NK FTSEVCISRICRIKEFFCLSMQFFGRKYF*MDN GLYNKLLEISCFR*ISIRFKINILSTF*VNEIK FETDFCSFCLYTNARRGGIIKNKICSESFHSVRL KLEETVVGPFVGGGQGSL*KASL*MIF*YIIVI ESFSCFLTWKVLDFKL*ICLFCRFKNMIFMGVV KLTVL*HLEVHFPSTNEIGLVLQNFRAVSESSDF ELSQFLFFLRPGLALP*ILQAISYFQ*IPFFLS LFVCLFLRWSFTLVA*AGVQWHDIGVPQPLHPR FKRFSCLSLPRSWDYRHVPPHPANFVFLVETGFF HVGQAGLELPTSGDLPTSASQSAGITGMSHRAR PSLHFYCSQSNIPIDCKISKPRVVEIYMYLFI* IETGS*TPRLK*SSCLNLPKCWDYRHELAGLLTF FF*L*QFLFYSEHNSIRLSLYI*LTNKSWGEGS MCKT*FRAAFFEKVGVFGCVTKQ*GDVFAV*QN NEGFKQDIFASYVKSK*GCCGGWFSSQ*CLFCIF PLCCP*HISNTFMIIVPACKRADIYMSARQEEK VAVPATSGKQFSHLSLPSS*DHRHEISQNSSQQ AFT*FHC*EL*QVHL*LQRRLRK*TQQSSLSLGN TFQEWIPETADSTEPYIYYVFAYVYILDMVWLL PNPKSHLEL*SPNPYVLRVGPGGDNWIMGAISP VLFLR**VTLRRSVGFISAWRFPCLQSLHLAAL* RCLLLLAFCHDCKFPEAFPAMRNCESVKPVSFI INQFWIFLHSSVRMD*YIPMIKFINWTQ*QINN KIEQL**YTIIKCMW

Sequence APC.fAug05

GGCGGAAAAGCGGGCTGTCTCGGAAACTCAGAGCCGGGTTCCTCCCGGGTTTCTGCCGGGTTTCTCCCTGCGGCTCCTGGGTTGTTGAGACTCTTGTGAA GATGGCTTGCGCTGCCGCGCGGTCCCCGGCCGACCAGGACAGGTTTATTTGTATCTATCCTGCTTATTTAAATAATAAGAAGACCATCGCAGAGGGAAGG CGAATCCCCATAAGTAAGGCTGTTGAAAATCCTACAGCTACAGAGATTCAAGATGTATGTTCAGCAGTTGGACTTAACGTATTTCTTGAGAAAAATAAAA TGTACTCTAGAGAATGGAATCGTGATGTCCAATACAGAGGCAGAGTCCGGGTCCAGCTCAAACAGGAAGATGGGAGCCTCTGCCTTGTACAGTTCCCATC ACGTAAGTCAGTAATGTTGTATGCAGCAGAAATGATACCTAAACTAAAAACAAGGACACAAAAAACAGGAGGTGCTGACCAAAGTCTTCAACAAGGAGAG GGAAGTAAAAAAGGGAAAGGAAAGAAAAAGAAGTAACCTAGTATCAGCATCAAGTATGTGGTACTACTGTAAGAGACATGAATGGAGACTTCTAATTTGT ATCGGAGGGAAACAGAAGCTTTTTGTTTGCATCATTTAACTGAACTGTGAACCCTTGTGCCTCTCATCTTTATCATCGGAGTTGACAGTGAAACAAATTT ACATCAGAAGTTTGCATCTCGCGTATATGCCGTATAAAAGAATTTTTTTGTCTTTCAATGCAGTTTTTTGGAAGAAAATATTTTTAAATGGACAATGGAC TGTACAATAAGTTACTTGAAATAAGTTGTTTCAGATAAATTTCAATTAGATTTAAAATAAACATTTTGTCCACCTTTTAAGTTAATGAAATAAAATTTGA AACTGACTTTTGCAGCTTTTGCTTATATACTAATGCTAGGAGAGGAGGGATAATTAAGAATAAAATATGTAGTGAAAGTTTCCATAGTGTGAGGCTAAAA CTAGAAGAAACTGTGGTAGGTCCTTTTGTGGGTGGGGGACAGGGGAGTCTGTAAAAAGCCAGTCTGTAGATGATATTTTAATATATTATTGTAATCGAAT CGTTCAGTTGTTTTTTGACATGGAAAGTCCTGGATTTTAAGCTTTAAATTTGCTTATTTTGTAGGTTTAAGAACATGATTTTCATGGGAGTTGTAAAATT AACTGTGCTTTAGCACCTTGAGGTACACTTTCCTTCAACAAATGAAATTGGATTGGTGCTCCAGAATTTCAGAGCGGTTTCTGAAAGTAGTGATTTTGAG CTATCCCAATTCCTGTTCTTCCTGAGGCCTGGTTTAGCTCTTCCTTGAATTCTGCAAGCTATCTCATATTTTCAGTAAATTCCATTTTTTTTAAGTTTGT TTGTTTGTTTGTTTTTGAGATGGAGTTTCACTCTTGTTGCCTAGGCTGGAGTGCAATGGCACGATATCGGCGTACCACAACCTCTGCATCCCAGGTTCAA GAGATTCTCCTGCCTCAGCCTCCCTAGGAGCTGGGATTACAGGCATGTGCCACCACACCCGGCTAATTTTGTATTTTTAGTAGAGACAGGGTTTTTCCAT GTTGGTCAGGCTGGTCTCGAACTCCCGACCTCAGGTGATCTGCCCACCTCAGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACCGTGCCCGGCCTT CTTTACATTTTTATTGCTCACAGTCAAACATTCCTATTGATTGTAAAATAAGCAAACCTAGAGTTGTGGAGATTTATATGTATTTATTTATTTGAATTGA GACAGGGTCTTAAACTCCCAGGCTCAAGTGATCCTCCTGCCTCAACCTCCCAAAGTGCTGGGATTACAGGCATGAGCTAGCTGGCCTGTTGACTTTTTTT TTTTAACTCTAACAATTTCTGTTTTATAGTGAACATAATAGTATAAGACTTAGTTTATATATTTGACTTACAAATAAATCCTGGGGAGAGGGGAGTATGT GTAAAACATAATTCAGAGCAGCCTTCTTTGAGAAAGTGGGTGTGTTTGGCTGTGTAACAAAACAATAAGGGGATGTGTTTGCTGTGTAACAAAACAATGA AGGATTTAAGCAGGATATTTTTGCCTCATATGTTAAATCCAAGTGAGGTTGCTGTGGTGGTTGGTTTAGCTCTCAGTGATGCCTTTTTTGTATTTTTCCG CTCTGCTGTCCATGACATATTTCTAACACCTTTATGATTATTGTTCCTGCTTGTAAAAGGGCTGATATTTACATGAGTGCAAGGCAGGAAGAAAAGGTAG CTGTGCCAGCCACTTCTGGCAAGCAGTTCTCCCACCTTAGCCTCCCAAGTAGCTGAGACCATAGGCATGAGATTTCTCAAAATTCCTCCCAGCAGGCTTT CACTTAGTTTCATTGTTGAGAACTGTGACAGGTCCATCTCTAGCTGCAAAGGAGGCTGAGAAAGTGAACACAGCAGTCCTCCTTATCCTTGGGGAATACA TTCCAAGAGTGGATCCCTGAAACAGCAGATAGTACTGAACCCTATATATACTATGTTTTTGCCTATGTATATATACTTGATATGGTTTGGCTGCTACCCA ACCCAAAATCTCATCTTGAATTATAATCCCCAAATCCCTATGTGTTAAGGGTGGGACCAGGTGGAGATAATTGGATCATGGGGGCAATTTCCCCTGTGCT GTTCTTGAGATAATGAGTGACTCTCAGGAGATCTGTTGGTTTTATAAGTGCCTGGCGTTTCCCCTGCTTGCAGTCACTCCATCTTGCTGCCCTGTGAAGG TGCCTGCTTCTCCTTGCCTTCTGCCATGATTGTAAGTTTCCTGAGGCCTTCCCAGCAATGCGGAACTGTGAGTCAGTTAAACCTGTTTCCTTTATAATTA ATCAGTTTTGGATATTTCTTCATAGCAGTGTGAGAATGGATTAATACATACCTATGATAAAGTTTATAAATTGGACACAGTAACAGATTAACAATAAAAT AGAACAATTATAATAATATACTATAATAAAATGTATGTGG

APC.F12 (forward primer)
APC.R11 (reverse primer)
Alternative acceptor

Frame 0

GGKAGCLGNSEPGSSRVSAGFLPAAPGLLRLL* RWLALPRGPRPTRTGLFVSILLI*IIRRPSQREG ESP*VRLLKILQLQRFKMYVQQLDLTYFLRKIK CTLENGIVMSNTEAESGSSSNRKMGASALYSSH HVSQ*CCMQQK*YLN*KQGHKKQEVLTKVFNKER EVKKGKERKRSNLVSASSMWYYCKRHEWRLLIC IGGKQKLFVCII*LNCEPLCLSSLSSELTVKQI YIRSLHLAYMPYKRIFLSFNAVFWKKIFLNGQWT VQ*VT*NKLFQINFN*I*NKHFVHLLS**NKI* N*LLQLLLIY*C*ERRDN*E*NM**KFP*CEAK TRRNCGRSFCGWGTGESVKSQSVDDILIYYCNRI VQLFFDMESPGF*ALNLLIL*V*EHDFHGSCKI NCALAP*GTLSFNK*NWIGAPEFQSGF*K**F* AIPIPVLPEAWFSSSLNSASYLIFSVNSIFFKFV CLFVFEMEFHSCCLGWSAMARYRRTTTSASQVQ EILLPQPP*ELGLQACATTPG*FCIFSRDRVFP CWSGWSRTPDLR*SAHLSLPKCWDYRHEPPCPAF FTFLLLTVKHSY*L*NKQT*SCGDLYVFIYLN* DRVLNSQAQVILLPQPPKVLGLQA*ASWPVDFF FLTLTISVL**T**YKT*FIYLTYK*ILGRGEYV *NIIQSSLL*ESGCVWLCNKTIRGCVCCVTKQ* RI*AGYFCLIC*IQVRLLWWLV*LSVMPFLYFS ALLSMTYF*HLYDYCSCL*KG*YLHECKAGRKGS CASHFWQAVLPP*PPK*LRP*A*DFSKFLPAGF HLVSLLRTVTGPSLAAKEAEKVNTAVLLILGEY IPRVDP*NSR*Y*TLYILCFCLCIYT*YGLAATQ PKISS*IIIPKSLCVKGGTRWR*LDHGGNFPCA VLEIMSDSQEICWFYKCLAFPLLAVTPSCCPVK VPASPCLLP*L*VS*GLPSNAEL*VS*TCFLYN* SVLDISS*QCENGLIHTYDKVYKLDTVTD*Q*N RTIIIIYYNKMYV

Frame 1

AEKRAVSETQSRVPPGFLPGFSLRLLGC*DSCE DGLRCRAVPGRPGQVYLYLSCLFK**EDHRRGK ANPHK*GC*KSYSYRDSRCMFSSWT*RIS*EK*N VL*RMES*CPIQRQSPGPAQTGRWEPLPCTVPI T*VSNVVCSRNDT*TKNKDTKNRRC*PKSSTRR GK*KRERKEKEVT*YQHQVCGTTVRDMNGDF*FV SEGNRSFLFASFN*TVNPCASHLYHRS*Q*NKF TSEVCISRICRIKEFFCLSMQFFGRKYF*MDNG LYNKLLEISCFR*ISIRFKINILSTF*VNEIKFE TDFCSFCLYTNARRGGIIKNKICSESFHSVRLK LEETVVGPFVGGGQGSL*KASL*MIF*YIIVIE SFSCFLTWKVLDFKL*ICLFCRFKNMIFMGVVKL TVL*HLEVHFPSTNEIGLVLQNFRAVSESSDFE LSQFLFFLRPGLALP*ILQAISYFQ*IPFFLSL FVCLFLRWSFTLVA*AGVQWHDIGVPQPLHPRFK RFSCLSLPRSWDYRHVPPHPANFVFLVETGFFH VGQAGLELPTSGDLPTSASQSAGITGMSHRARP SLHFYCSQSNIPIDCKISKPRVVEIYMYLFI*IE TGS*TPRLK*SSCLNLPKCWDYRHELAGLLTFF F*L*QFLFYSEHNSIRLSLYI*LTNKSWGEGSM CKT*FRAAFFEKVGVFGCVTKQ*GDVFAV*QNNE GFKQDIFASYVKSK*GCCGGWFSSQ*CLFCIFP LCCP*HISNTFMIIVPACKRADIYMSARQEEKV AVPATSGKQFSHLSLPSS*DHRHEISQNSSQQAF T*FHC*EL*QVHL*LQRRLRK*TQQSSLSLGNT FQEWIPETADSTEPYIYYVFAYVYILDMVWLLP NPKSHLEL*SPNPYVLRVGPGGDNWIMGAISPVL FLR**VTLRRSVGFISAWRFPCLQSLHLAAL*R CLLLLAFCHDCKFPEAFPAMRNCESVKPVSFII NQFWIFLHSSVRMD*YIPMIKFINWTQ*QINNKI EQL**YTIIKCMW

Frame 2

RKSGLSRKLRAGFLPGFCRVSPCGSWVVETLVK MACAAARSPADQDRFICIYPAYLNNKKTIAEGR RIPISKAVENPTATEIQDVCSAVGLNVFLEKNK MYSREWNRDVQYRGRVRVQLKQEDGSLCLVQFPS RKSVMLYAAEMIPKLKTRTQKTGGADQSLQQGE GSKKGKGKKKK*PSISIKYVVLL*ET*METSNL YRRETEAFCLHHLTEL*TLVPLIFIIGVDSETNL HQKFASRVYAV*KNFFVFQCSFLEENIFKWTMD CTISYLK*VVSDKFQLDLK*TFCPPFKLMK*NL KLTFAAFAYILMLGEEG*LRIKYVVKVSIV*G*N *KKLW*VLLWVGDRGVCKKPVCR*YFNILL*SN RSVVF*HGKSWILSFKFAYFVGLRT*FSWEL*N *LCFSTLRYTFLQQMKLDWCSRISERFLKVVILS YPNSCSS*GLV*LFLEFCKLSHIFSKFHFF*VC LFVCF*DGVSLLLPRLECNGTISAYHNLCIPGS RDSPASASLGAGITGMCHHTRLILYF**RQGFSM LVRLVSNSRPQVICPPQPPKVLGLQA*ATVPGL LYIFIAHSQTFLLIVK*ANLELWRFICIYLFEL RQGLKLPGSSDPPASTSQSAGITGMS*LAC*LFF FNSNNFCFIVNIIV*DLVYIFDLQINPGERGVC VKHNSEQPSLRKWVCLAV*QNNKGMCLLCNKTM KDLSRIFLPHMLNPSEVAVVVGLALSDAFFVFFR SAVHDIFLTPL*LLFLLVKGLIFT*VQGRKKR* LCQPLLASSSPTLASQVAETIGMRFLKIPPSRL SLSFIVENCDRSISSCKGG*ESEHSSPPYPWGIH SKSGSLKQQIVLNPIYTMFLPMYIYLIWFGCYP TQNLILNYNPQIPMC*GWDQVEIIGSWGQFPLC CS*DNE*LSGDLLVL*VPGVSPACSHSILLPCEG ACFSLPSAMIVSFLRPSQQCGTVSQLNLFPL*L ISFGYFFIAV*EWINTYL**SL*IGHSNRLTIK *NNYNNIL**NVC

Sequence APC.hAug05

APC.F12 (forward primer)
APC.R11 (reverse primer)
Alternative acceptor

Frame 0

GGKAGCLGNSEPGSSRVSAGFLPAAPGLLRLL* RWLALPRGPRPTRTGLFVSILLI*IIRRPSQREG ESP*VRLLKILQLQRFKMYVQQLDLTYFLRKIK CTLENGIVMSNTEAESGSSSNRKMGASALYSSH HIIH*A*LLVPR*VSNVVCSRNDT*TKNKDTKNR RC*PKSSTRRGK*KRERKEKEVT*YQHQVCGTT VRDMNGDF*FVSEGNRSFLFASFN*TVNPCASH LYHRS*Q*NKFTSEVCISRICRIKEFFCLSMQFF GRKYF*MDNGLYNKLLEISCFR*ISIRFKINIL STF*VNEIKFETD

Frame 1

AEKRAVSETQSRVPPGFLPGFSLRLLGC*DSCE DGLRCRAVPGRPGQVYLYLSCLFK**EDHRRGK ANPHK*GC*KSYSYRDSRCMFSSWT*RIS*EK*N VL*RMES*CPIQRQSPGPAQTGRWEPLPCTVPI TLYTKPNFWFLGKSVMLYAAEMIPKLKTRTQKT GGADQSLQQGEGSKKGKGKKKK*PSISIKYVVLL *ET*METSNLYRRETEAFCLHHLTEL*TLVPLI FIIGVDSETNLHQKFASRVYAV*KNFFVFQCSF LEENIFKWTMDCTISYLK*VVSDKFQLDLK*TFC PPFKLMK*NLKL

Frame 2

RKSGLSRKLRAGFLPGFCRVSPCGSWVVETLVK MACAAARSPADQDRFICIYPAYLNNKKTIAEGR RIPISKAVENPTATEIQDVCSAVGLNVFLEKNK MYSREWNRDVQYRGRVRVQLKQEDGSLCLVQFPS HYTLSLTSGS*VSQ*CCMQQK*YLN*KQGHKKQ EVLTKVFNKEREVKKGKERKRSNLVSASSMWYY CKRHEWRLLICIGGKQKLFVCII*LNCEPLCLSS LSSELTVKQIYIRSLHLAYMPYKRIFLSFNAVF WKKIFLNGQWTVQ*VT*NKLFQINFN*I*NKHF VHLLS**NKI*N*