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Sequences

Sequence MAP2K4.gAug05

MAP2K4.F6 (forward primer)
MAP2K4.R3 (reverse primer)
Single exon skipping

Frame 0

GRARGRACCIAAAAAPLGSSLPTMAAPSPSGGG GSGGGSGSGTPGPVGSPAPGHPAVSSMQGKRKAL KLNFANPPFKSTARFTLNPNPTGVQNPHIEFGQ QWMKKNKNNFLWIWM**CGVVIAHTLFSFMVHS SERVTVGSVWNSCLPRLISFTNMYIVY*MMLFQK KF*AKSL*Q*VPGL*IIHCVYSYIEMLLEF*MH IFECHSQYLPENNS*LITRDKVTSVG*LTQ*VI MVSINTANVLSL*ISMSHNRLFCK*S*EKQFSVH FFLLL*LFLNRLYYHSYPEINQNHSSLLEAP*L KPLDKVCLL*FKILVTP*LKPLDKVC

Frame 1

AVREAELAALQPPRRRSALHSQQWRLRARAAAA APGAAAAAAPPAP*GPRRQATRPSAACRVNAKH *S*ILQIHLSNLQQGLL*IPILQEFKTHT*NSVN SG*KRTKTTSYGFGCSNAE**LPIHCSVLWCTL QRG*LLDLYGTHVYLV**VLQICI*CIR*CYSR RNFRQNHFSSKYLVFKLFIVYIVI*RCCWSFECT YLSVTHSTFLKIIHS**PETKSLLWGS*HNRS* WSR*ILPMFFPFESQ*VITDCSVNKARKSNFLF IFSCCFNYF*IGYITTVILKLIRITAVSLKPHN* SPWTKFVYCDLRFWLLHN*SPWTKY

Frame 2

PCERPSLLHCSRRGAARLFTPNNGGSEPERRRR LRGRQRQRHPRPRRVPGARPPGRQQHAG*TQST EVEFCKSTFQIYSKVYSESQSYRSSKPTHRIRS TVDEKEQKQLLMDLDVVMRSSDCPYIVQFYGALF REGDCWICMELMSTSFDKFYKYVYSVLDDVIPE EILGKITLAVSTWSLNYSLCI*LYRDAAGVLNA HI*VSLTVPS*K*FIVNNQRQSHFCGVVNTIGHN GLDKYCQCSFPLNLNES*QTVL*IKLGKAIFCS FFPVALTIFK*VILPQLS*N*SESQQSP*SPII EALGQSLFTVI*DFGYSIIEALGQSM

Sequence MAP2K4.aAug05

GGCCGTGCGAGAGGCCGAGCTTGCTGCATTGCAGCCGCCGCGGCGCCGCTCGGCTCTTCACTCCCAACAATGGCGGCTCCGAGCCCGAGCGGCGGCGGCG GCTCCGGGGGCGGCAGCGGCAGCGGCACCCCCGGCCCCGTAGGGTCCCCGGCGCCAGGCCACCCGGCCGTCAGCAGCATGCAGGGCTTTCAGATAAACTT CTGTGAAAAGGCACAAAGTAAACGCAAAGCACTGAAGTTGAATTTTGCAAATCCACCTTTCAAATCTACAGCAAGGTTTACTCTGAATCCCAATCCTACA GGAGTTCAAAACCCACACATAGAGAGACTGAGAACACACAGCATTGAGTCATCAGGAAAACTGAAGATCTCCCCTGAACAACACTGGGATTTCACTGCAG AGGACTTGAAAGACCTTGGAGAAATTGGACGAGGAGCTTATGGTTCTGTCAACAAAATGGTCCACAAACCAAGTGGGCAAATAATGGCAGTTAAAAGAAT TCGGTCAACAGTGGATGAAAAAGAACAAAAACAACTTCTTATGGATTTGGATGTAGTAATGCGGAGTAGTGATTGCCCATACATTGTTCAGTTTTATGGT GCACTCTTCAGAGAGGGTGACTGTTGGATCTGTATGGAACTCATGTCTACCTCGTTTGATAAGTTTTACAAATATGTATATAGTGTATTAGATGATGTTA TTCCAGAAGAAATTTTAGGCAAAATCACTTTAGCAACTGTGAAAGCACTAAACCACTTAAAAGAAAACTTGAAAATTATTCACAGAGATATCAAACCTTC CAATATTCTTCTGGACAGAAGTGGAAATATTAAGCTCTGTGACTTCGGCATCAGTGGACAGCTTGTGGACTCTATTGCCAAGACAAGAGATGCTGGCTGT AGGCCATACATGGCACCTGAAAGAATAGACCCAAGCGCATCACGACAAGGATATGATGTCCGCTCTGATGTCTGGAGTTTGGGGATCACATTGTATGAGT TGGCCACAGGCCGATTTCCTTATCCAAAGTGGAATAGTGTATTTGATCAACTAACACAAGTCGTGAAAGGAGATCCTCCGCAGCTGAGTAATTCTGAGGA AAGGGAATTCTCCCCGAGTTTCATCAACTTTGTCAACTTGTGCCTTACGAAGGATGAATCCAAAAGGCCAAAGTATAAAGAGCTTCTGAAACATCCCTTT ATTTTGATGTATGAAGAACGTGCCGTTGAGGTCGCATGCTATGTTTGTAAAATCCTGGATCAAATGCCAGCTACTCCCAGCTCTCCCATGTATGTCGATT GATATCGCTGCTACATCAGACTCTAGAAAAAAGGGCTGAGAGGAAGCAAGACGTAAAGAATTTTCATCCCGTATCACAGTGTTTTTATTGCTCGCCCAGA CACCATGTGCAATAAGATTGGTGTTCGTTTCCATCATGTCTGTATACTCCTGTCACCTAGAACGTGCATCCTTGTAATACCTGATTGATCACACAGTGTT AGTGCTGGTCAGAGAGACCTCATCCTGCTCTTTTGTGATGAACATATTCATGAAATGTGGAAGTCAGTACGATCAAGTTGTTGACTGTGATTAGATCACA TCTTAAATTCATTTCTAGACTCAAAACCTGGAGATGCAGCTACTGGAATGGTGTTTTGTCAGACTTCCAAATCCTGGAAGGACACAGTGATGAATGTACT ATGTCTGAACATAGAAACTCGGGCTTGAGTGAGAAGAGCTTGCACAGCCAACGAGACACATTGCCTTCTGGAGCTGGGAGACAAAGGAGGAATTTACTTT CTTCACCAAGTGCAATAGATTACTGATGTGATATTCTGTTGCTTTACAGTTACAGTTGATGTTTGGGGATCGATGTGCTCAGCCAAATTTCCTGTTTGAA ATATCATGTTAAATTAGAATGAATTTATCTTTACCAAAAACCATGTTGCGTTCAAAGAGGTGAACATTAAAATATAGAGACAGGACAGAATGTGTTCTTT TCTCCTTTACCAGTCCTATTTTTCAATGGGAAGACTCAGGAGTCTGCCACTTGTCAAAGAAGGTGCTGATCCTAAGAATTTTTCATTCTCAGAATTCGGT GTGCTGCCAACTTGATGTTCCACCTGCCACAAACCACCAGGACTGAAAGAAGAAAACAGTACAGAAGGCAAAGTTTACAGATGTTTTTAATTCTAGTATT TTATCTGGAACAACTTGTAGCAGCTATATATTTCCCCTTGGTCCCAAGCCTGATACTTTAGCCATCATAACTCACTAACAGGGAGAAGTAGCTAGTAGCA ATGTGCCTTGATTGATTAGATAAAGATTTCTAGTAGGCAGCAAAAGACCAAATCTCAGTTGTTTGCTTCTTGCCATCACTGGTCCAGGTCTTCAGTTTCC GAATCTCTTTCCCTTCCCCTGTGGTCTATTGTCGCTATGTGACTTGCGCTTAATCCAATATTTTGCCTTTTTTCTATATCAAAAAACCTTTACAGTTAGC AGGGATGTTCCTTACCAAGGATTTTTAGCCCCAAATCTCTCATATTCGCTAGTGTTTAAAAGGCTAAGAATAGTGGGGCCCAGCCGATGTGGTAGGTGAT AAAGAGGCATCTTTTCTAGAGACACATTGGACCAGATGAGGATCCGAAACGGCAGCCTTTACGTTCATCACCTGCTAGAACCTCTCGTAGTCCATCACCA TTTCTTGGCATTGGAATTCTACTGGAAAAAAATACAAAAAGCAAAACAAAACCCTCAGCACTGTTACAAGAGGCCATTTAAGTATCTTGTGCTTCTTCAC TTACCCATTAGCCAGGTTCTCATTAGGTTTTGCTTGGGCCTCCCTGGCACTGAACCTTAGGCTTTGTATGACAGTGAAGCAGCACTGTGAGTGGTTCAAG CACACTGGAATATAAAACAGTCATGGCCTGAGATGCAGGTGATGCCATTACAGAACCAAATCGTGGCACGTATTGCTGTGTCTCCTCTCAGAGTGACAGT CATAAATACTGTCAAACAATAAAGGGAGAATGGTGCTGTTTAAAGTCACATCCCTGTAAATTGCAGAATTCAAAAGTGATTATCTCTTTGATCTACTTGC CTCATTTCCCTATCTTCTCCCCCACGGTATCCTAAACTTTAGACTTCCCACTGTTCTGAAAGGAGACATTGCTCTATGTCTGCCTTCGACCACAGCAAGC CATCATCCTCCATTGCTCCCGGGGACTCAAGAGGAATCTGTTTCTCTGCTGTCAACTTCCCATCTGGCTCAGCATAGGGTCACTTTGCCATTATGCAAAT GGAGATAAAAGCAATTCTGACTGTCCAGGAGCTAATCTGACCGTTCTATTGTGTGGATGACCACATAAGAAGGCAATTTTAGTGTATTAATCATAGATTA TTATAAACTATAAACTTAAGGGCAAGGAGTTTATTACAATGTATCTTTATTAAAACAAAAGGGTGTATAGTGTTCACAAACTGTGAAAATAGTGTAAGAA CTGTACATTGTGAGCTCTGGTTATTTTTCTCTTGTACCATAGAAAAATGTATAAAAATTATCAAAAAGCTAATGTGCAGGGATATTGCCTTATTTGTCTG TAAAAAATGGAGCTCAGTAACATAACTGCTTCTTGGAGCTTTGGAATATTTTATCCTGTATTCTTGTTTGAATTCCTCCTCTATTTAAGATATATACATG GAATCGAAGTGTTTATGTAATAGTTCTATCCTTTTGCCTGCAGGTCAGTTGTAATAAATCTAGGATGTGATGATGACTTTGTAATTTGATTTTCTGAAAT CAGACCCTGAGAGGGGAAAATCTTAAAGTAAATTACATTAAATTATCTGTGCATTTCACACCAGGGAAAATGA

MAP2K4.F6 (forward primer)
MAP2K4.R3 (reverse primer)
Single exon skipping

Frame 0

GRARGRACCIAAAAAPLGSSLPTMAAPSPSGGG GSGGGSGSGTPGPVGSPAPGHPAVSSMQGFQINF CEKAQSKRKALKLNFANPPFKSTARFTLNPNPT GVQNPHIERLRTHSIESSGKLKISPEQHWDFTA EDLKDLGEIGRGAYGSVNKMVHKPSGQIMAVKRI RSTVDEKEQKQLLMDLDVVMRSSDCPYIVQFYG ALFREGDCWICMELMSTSFDKFYKYVYSVLDDV IPEEILGKITLATVKALNHLKENLKIIHRDIKPS NILLDRSGNIKLCDFGISGQLVDSIAKTRDAGC RPYMAPERIDPSASRQGYDVRSDVWSLGITLYE LATGRFPYPKWNSVFDQLTQVVKGDPPQLSNSEE REFSPSFINFVNLCLTKDESKRPKYKELLKHPF ILMYEERAVEVACYVCKILDQMPATPSSPMYVD *YRCYIRL*KKGLRGSKT*RIFIPYHSVFIARPD TMCNKIGVRFHHVCILLSPRTCILVIPD*SHSV SAGQRDLILLFCDEHIHEMWKSVRSSC*L*LDH ILNSFLDSKPGDAATGMVFCQTSKSWKDTVMNVL CLNIETRA*VRRACTANETHCLLELGDKGGIYF LHQVQ*ITDVIFCCFTVTVDVWGSMCSAKFPV* NIMLN*NEFIFTKNHVAFKEVNIKI*RQDRMCSF LLYQSYFSMGRLRSLPLVKEGADPKNFSFSEFG VLPT*CSTCHKPPGLKEENSTEGKVYRCF*F*Y FIWNNL*QLYISPWSQA*YFSHHNSLTGRSS**Q CALID*IKISSRQQKTKSQLFASCHHWSRSSVS ESLSLPLWSIVAM*LALNPIFCLFSISKNLYS* QGCSLPRIFSPKSLIFASV*KAKNSGAQPMW*VI KRHLF*RHIGPDEDPKRQPLRSSPARTSRSPSP FLGIGILLEKNTKSKTKPSALLQEAI*VSCASS LTH*PGSH*VLLGPPWH*TLGFV*Q*SSTVSGSS TLEYKTVMA*DAGDAITEPNRGTYCCVSSQSDS HKYCQTIKGEWCCLKSHPCKLQNSKVIISLIYL PHFPIFSPTVS*TLDFPLF*KETLLYVCLRPQQA IILHCSRGLKRNLFLCCQLPIWLSIGSLCHYAN GDKSNSDCPGANLTVLLCG*PHKKAILVY*S*I IINYKLKGKEFITMYLY*NKRVYSVHKL*K*CKN CTL*ALVIFLLYHRKMYKNYQKANVQGYCLICL *KMELSNITASWSFGIFYPVFLFEFLLYLRYIH GIEVFM**FYPFACRSVVINLGCDDDFVI*FSEI RP*EGKILK*ITLNYLCISHQGK*

Frame 1

AVREAELAALQPPRRRSALHSQQWRLRARAAAA APGAAAAAAPPAP*GPRRQATRPSAACRAFR*T SVKRHKVNAKH*S*ILQIHLSNLQQGLL*IPILQ EFKTHT*RD*EHTALSHQEN*RSPLNNTGISLQ RT*KTLEKLDEELMVLSTKWSTNQVGK*WQLKE FGQQWMKKNKNNFLWIWM**CGVVIAHTLFSFMV HSSERVTVGSVWNSCLPRLISFTNMYIVY*MML FQKKF*AKSL*QL*KH*TT*KKT*KLFTEISNL PIFFWTEVEILSSVTSASVDSLWTLLPRQEMLAV GHTWHLKE*TQAHHDKDMMSALMSGVWGSHCMS WPQADFLIQSGIVYLIN*HKS*KEILRS*VILR KGNSPRVSSTLSTCALRRMNPKGQSIKSF*NIPL F*CMKNVPLRSHAMFVKSWIKCQLLPALPCMSI DIAATSDSRKKG*EEARRKEFSSRITVFLLLAQ TPCAIRLVFVSIMSVYSCHLERASL*YLIDHTVL VLVRETSSCSFVMNIFMKCGSQYDQVVDCD*IT S*IHF*TQNLEMQLLEWCFVRLPNPGRTQ**MY YV*T*KLGLE*EELAQPTRHIAFWSWETKEEFTF FTKCNRLLM*YSVALQLQLMFGDRCAQPNFLFE ISC*IRMNLSLPKTMLRSKR*TLKYRDRTECVL FSFTSPIFQWEDSGVCHLSKKVLILRIFHSQNSV CCQLDVPPATNHQD*KKKTVQKAKFTDVFNSSI LSGTTCSSYIFPLGPKPDTLAIITH*QGEVASS NVP*LIR*RFLVGSKRPNLSCLLLAITGPGLQFP NLFPFPCGLLSLCDLRLIQYFAFFLYQKTFTVS RDVPYQGFLAPNLSYSLVFKRLRIVGPSRCGR* *RGIFSRDTLDQMRIRNGSLYVHHLLEPLVVHHH FLALEFYWKKIQKAKQNPQHCYKRPFKYLVLLH LPISQVLIRFCLGLPGTEP*ALYDSEAAL*VVQ AHWNIKQSWPEMQVMPLQNQIVARIAVSPLRVTV INTVKQ*RENGAV*SHIPVNCRIQK*LSL*STC LISLSSPPRYPKL*TSHCSERRHCSMSAFDHSK PSSSIAPGDSRGICFSAVNFPSGSA*GHFAIMQM EIKAILTVQELI*PFYCVDDHIRRQF*CINHRL L*TINLRARSLLQCIFIKTKGCIVFTNCENSVR TVHCELWLFFSCTIEKCIKIIKKLMCRDIALFVC KKWSSVT*LLLGALEYFILYSCLNSSSI*DIYM ESKCLCNSSILLPAGQL**I*DVMMTL*FDFLK SDPERGKS*SKLH*IICAFHTREN

Frame 2

PCERPSLLHCSRRGAARLFTPNNGGSEPERRRR LRGRQRQRHPRPRRVPGARPPGRQQHAGLSDKL L*KGTK*TQSTEVEFCKSTFQIYSKVYSESQSY RSSKPTHRETENTQH*VIRKTEDLP*TTLGFHCR GLERPWRNWTRSLWFCQQNGPQTKWANNGS*KN SVNSG*KRTKTTSYGFGCSNAE**LPIHCSVLW CTLQRG*LLDLYGTHVYLV**VLQICI*CIR*CY SRRNFRQNHFSNCESTKPLKRKLENYSQRYQTF QYSSGQKWKY*AL*LRHQWTACGLYCQDKRCWL *AIHGT*KNRPKRITTRI*CPL*CLEFGDHIV*V GHRPISLSKVE*CI*STNTSRERRSSAAE*F*G KGILPEFHQLCQLVPYEG*IQKAKV*RASETSL YFDV*RTCR*GRMLCL*NPGSNASYSQLSHVCRL ISLLHQTLEKRAERKQDVKNFHPVSQCFYCSPR HHVQ*DWCSFPSCLYTPVT*NVHPCNT*LITQC *CWSERPHPALL**TYS*NVEVSTIKLLTVIRSH LKFISRLKTWRCSYWNGVLSDFQILEGHSDECT MSEHRNSGLSEKSLHSQRDTLPSGAGRQRRNLL SSPSAIDY*CDILLLYSYS*CLGIDVLSQISCLK YHVKLE*IYLYQKPCCVQRGEH*NIETGQNVFF SPLPVLFFNGKTQESATCQRRC*S*EFFILRIR CAANLMFHLPQTTRTERRKQYRRQSLQMFLILVF YLEQLVAAIYFPLVPSLIL*PS*LTNREK*LVA MCLD*LDKDF**AAKDQISVVCFLPSLVQVFSF RISFPSPVVYCRYVTCA*SNILPFFYIKKPLQLA GMFLTKDF*PQISHIR*CLKG*E*WGPADVVGD KEASFLETHWTR*GSETAAFTFITC*NLS*SIT ISWHWNSTGKKYKKQNKTLSTVTRGHLSILCFFT YPLARFSLGFAWASLALNLRLCMTVKQHCEWFK HTGI*NSHGLRCR*CHYRTKSWHVLLCLLSE*Q S*ILSNNKGRMVLFKVTSL*IAEFKSDYLFDLLA SFPYLLPHGILNFRLPTVLKGDIALCLPSTTAS HHPPLLPGTQEESVSLLSTSHLAQHRVTLPLCK WR*KQF*LSRS*SDRSIVWMTT*EGNFSVLIIDY YKL*T*GQGVYYNVSLLKQKGV*CSQTVKIV*E LYIVSSGYFSLVP*KNV*KLSKS*CAGILPYLS VKNGAQ*HNCFLELWNILSCILV*IPPLFKIYTW NRSVYVIVLSFCLQVSCNKSRM***LCNLIF*N QTLRGENLKVNYIKLSVHFTPGKM

Sequence MAP2K4.bAug05

GGCCGTGCGAGAGGCCGAGCTTGCTGCATTGCAGCCGCCGCGGCGCCGCTCGGCTCTTCACTCCCAACAATGGCGGCTCCGAGCCCGAGCGGCGGCGGCG GCTCCGGGGGCGGCAGCGGCAGCGGCACCCCCGGCCCCGTAGGGTCCCCGGCGCCAGGCCACCCGGCCGTCAGCAGCATGCAGGGTAAACGCAAAGCACT GAAGTTGAATTTTGCAAATCCACCTTTCAAATCTACAGCAAGGTTTACTCTGAATCCCAATCCTACAGGAGTTCAAAACCCACACATAGAGAGACTGAGA ACACACAGCATTGAGTCATCAGGAAAACTGAAGATCTCCCCTGAACAACACTGGGATTTCACTGCAGAGGACTTGAAAGACCTTGGAGAAATTGGACGAG GAGCTTATGGTTCTGTCAACAAAATGGTCCACAAACCAAGTGGGCAAATAATGGCAGTTAAAAGAATTCGGTCAACAGTGGATGAAAAAGAACAAAAACA ACTTCTTATGGATTTGGATGTAGTAATGCGGAGTAGTGATTGCCCATACATTGTTCAGTTTTATGGTGCACTCTTCAGAGAGGGTGACTGTTGGATCTGT ATGGAACTCATGTCTACCTCGTTTGATAAGTTTTACAAATATGTATATAGTGTATTAGATGATGTTATTCCAGAAGAAATTTTAGGCAAAATCACTTTAG CAACTGTGAAAGCACTAAACCACTTAAAAGAAAACTTGAAAATTATTCACAGAGATATCAAACCTTCCAATATTCTTCTGGACAGAAGTGGAAATATTAA GCTCTGTGACTTCGGCATCAGTGGACAGCTTGTGGACTCTATTGCCAAGACAAGAGATGCTGGCTGTAGGCCATACATGGCACCTGAAAGAATAGACCCA AGCGCATCACGACAAGGATATGATGTCCGCTCTGATGTCTGGAGTTTGGGGATCACATTGTATGAGTTGGCCACAGGCCGATTTCCTTATCCAAAGTGGA ATAGTGTATTTGATCAACTAACACAAGTCGTGAAAGGAGATCCTCCGCAGCTGAGTAATTCTGAGGAAAGGGAATTCTCCCCGAGTTTCATCAACTTTGT CAACTTGTGCCTTACGAAGGATGAATCCAAAAGGCCAAAGTATAAAGAGCTTCTGAAACATCCCTTTATTTTGATGTATGAAGAACGTGCCGTTGAGGTC GCATGCTATGTTTGTAAAATCCTGGATCAAATGCCAGCTACTCCCAGCTCTCCCATGTATGTCGATTGATATCGCTGCTACATCAGACTCTAGAAAAAAG GGCTGAGAGGAAGCAAGACGTAAAGAATTTTCATCCCGTATCACAGTGTTTTTATTGCTCGCCCAGACACCATGTGCAATAAGATTGGTGTTCGTTTCCA TCATGTCTGTATACTCCTGTCACCTAGAACGTGCATCCTTGTAATACCTGATTGATCACACAGTGTTAGTGCTGGTCAGAGAGACCTCATCCTGCTCTTT TGTGATGAACATATTCATGAAATGTGGAAGTCAGTACGATCAAGTTGTTGACTGTGATTAGATCACATCTTAAATTCATTTCTAGACTCAAAACCTGGAG ATGCAGCTACTGGAATGGTGTTTTGTCAGACTTCCAAATCCTGGAAGGACACAGTGATGAATGTACTATGTCTGAACATAGAAACTCGGGCTTGAGTGAG AAGAGCTTGCACAGCCAACGAGACACATTGCCTTCTGGAGCTGGGAGACAAAGGAGGAATTTACTTTCTTCACCAAGTGCAATAGATTACTGATGTGATA TTCTGTTGCTTTACAGTTACAGTTGATGTTTGGGGATCGATGTGCTCAGCCAAATTTCCTGTTTGAAATATCATGTTAAATTAGAATGAATTTATCTTTA CCAAAAACCATGTTGCGTTCAAAGAGGTGAACATTAAAATATAGAGACAGGACAGAATGTGTTCTTTTCTCCTTTACCAGTCCTATTTTTCAATGGGAAG ACTCAGGAGTCTGCCACTTGTCAAAGAAGGTGCTGATCCTAAGAATTTTTCATTCTCAGAATTCGGTGTGCTGCCAACTTGATGTTCCACCTGCCACAAA CCACCAGGACTGAAAGAAGAAAACAGTACAGAAGGCAAAGTTTACAGATGTTTTTAATTCTAGTATTTTATCTGGAACAACTTGTAGCAGCTATATATTT CCCCTTGGTCCCAAGCCTGATACTTTAGCCATCATAACTCACTAACAGGGAGAAGTAGCTAGTAGCAATGTGCCTTGATTGATTAGATAAAGATTTCTAG TAGGCAGCAAAAGACCAAATCTCAGTTGTTTGCTTCTTGCCATCACTGGTCCAGGTCTTCAGTTTCCGAATCTCTTTCCCTTCCCCTGTGGTCTATTGTC GCTATGTGACTTGCGCTTAATCCAATATTTTGCCTTTTTTCTATATCAAAAAACCTTTACAGTTAGCAGGGATGTTCCTTACCAAGGATTTTTAGCCCCA AATCTCTCATATTCGCTAGTGTTTAAAAGGCTAAGAATAGTGGGGCCCAGCCGATGTGGTAGGTGATAAAGAGGCATCTTTTCTAGAGACACATTGGACC AGATGAGGATCCGAAACGGCAGCCTTTACGTTCATCACCTGCTAGAACCTCTCGTAGTCCATCACCATTTCTTGGCATTGGAATTCTACTGGAAAAAAAT ACAAAAAGCAAAACAAAACCCTCAGCACTGTTACAAGAGGCCATTTAAGTATCTTGTGCTTCTTCACTTACCCATTAGCCAGGTTCTCATTAGGTTTTGC TTGGGCCTCCCTGGCACTGAACCTTAGGCTTTGTATGACAGTGAAGCAGCACTGTGAGTGGTTCAAGCACACTGGAATATAAAACAGTCATGGCCTGAGA TGCAGGTGATGCCATTACAGAACCAAATCGTGGCACGTATTGCTGTGTCTCCTCTCAGAGTGACAGTCATAAATACTGTCAAACAATAAAGGGAGAATGG TGCTGTTTAAAGTCACATCCCTGTAAATTGCAGAATTCAAAAGTGATTATCTCTTTGATCTACTTGCCTCATTTCCCTATCTTCTCCCCCACGGTATCCT AAACTTTAGACTTCCCACTGTTCTGAAAGGAGACATTGCTCTATGTCTGCCTTCGACCACAGCAAGCCATCATCCTCCATTGCTCCCGGGGACTCAAGAG GAATCTGTTTCTCTGCTGTCAACTTCCCATCTGGCTCAGCATAGGGTCACTTTGCCATTATGCAAATGGAGATAAAAGCAATTCTGACTGTCCAGGAGCT AATCTGACCGTTCTATTGTGTGGATGACCACATAAGAAGGCAATTTTAGTGTATTAATCATAGATTATTATAAACTATAAACTTAAGGGCAAGGAGTTTA TTACAATGTATCTTTATTAAAACAAAAGGGTGTATAGTGTTCACAAACTGTGAAAATAGTGTAAGAACTGTACATTGTGAGCTCTGGTTATTTTTCTCTT GTACCATAGAAAAATGTATAAAAATTATCAAAAAGCTAATGTGCAGGGATATTGCCTTATTTGTCTGTAAAAAATGGAGCTCAGTAACATAACTGCTTCT TGGAGCTTTGGAATATTTTATCCTGTATTCTTGTTTGAATTCCTCCTCTATTTAAGATATATACATGGAATCGAAGTGTTTATGTAATAGTTCTATCCTT TTGCCTGCAGGTCAGTTGTAATAAATCTAGGATGTGATGATGACTTTGTAATTTGATTTTCTGAAATCAGACCCTGAGAGGGGAAAATCTTAAAGTAAAT TACATTAAATTATCTGTGCATTTCACACCAGGGAAAATGA

MAP2K4.F6 (forward primer)
MAP2K4.R3 (reverse primer)
Single exon skipping

Frame 0

GRARGRACCIAAAAAPLGSSLPTMAAPSPSGGG GSGGGSGSGTPGPVGSPAPGHPAVSSMQGKRKAL KLNFANPPFKSTARFTLNPNPTGVQNPHIERLR THSIESSGKLKISPEQHWDFTAEDLKDLGEIGR GAYGSVNKMVHKPSGQIMAVKRIRSTVDEKEQKQ LLMDLDVVMRSSDCPYIVQFYGALFREGDCWIC MELMSTSFDKFYKYVYSVLDDVIPEEILGKITL ATVKALNHLKENLKIIHRDIKPSNILLDRSGNIK LCDFGISGQLVDSIAKTRDAGCRPYMAPERIDP SASRQGYDVRSDVWSLGITLYELATGRFPYPKW NSVFDQLTQVVKGDPPQLSNSEEREFSPSFINFV NLCLTKDESKRPKYKELLKHPFILMYEERAVEV ACYVCKILDQMPATPSSPMYVD*YRCYIRL*KK GLRGSKT*RIFIPYHSVFIARPDTMCNKIGVRFH HVCILLSPRTCILVIPD*SHSVSAGQRDLILLF CDEHIHEMWKSVRSSC*L*LDHILNSFLDSKPG DAATGMVFCQTSKSWKDTVMNVLCLNIETRA*VR RACTANETHCLLELGDKGGIYFLHQVQ*ITDVI FCCFTVTVDVWGSMCSAKFPV*NIMLN*NEFIF TKNHVAFKEVNIKI*RQDRMCSFLLYQSYFSMGR LRSLPLVKEGADPKNFSFSEFGVLPT*CSTCHK PPGLKEENSTEGKVYRCF*F*YFIWNNL*QLYI SPWSQA*YFSHHNSLTGRSS**QCALID*IKISS RQQKTKSQLFASCHHWSRSSVSESLSLPLWSIV AM*LALNPIFCLFSISKNLYS*QGCSLPRIFSP KSLIFASV*KAKNSGAQPMW*VIKRHLF*RHIGP DEDPKRQPLRSSPARTSRSPSPFLGIGILLEKN TKSKTKPSALLQEAI*VSCASSLTH*PGSH*VL LGPPWH*TLGFV*Q*SSTVSGSSTLEYKTVMA*D AGDAITEPNRGTYCCVSSQSDSHKYCQTIKGEW CCLKSHPCKLQNSKVIISLIYLPHFPIFSPTVS *TLDFPLF*KETLLYVCLRPQQAIILHCSRGLKR NLFLCCQLPIWLSIGSLCHYANGDKSNSDCPGA NLTVLLCG*PHKKAILVY*S*IIINYKLKGKEF ITMYLY*NKRVYSVHKL*K*CKNCTL*ALVIFLL YHRKMYKNYQKANVQGYCLICL*KMELSNITAS WSFGIFYPVFLFEFLLYLRYIHGIEVFM**FYP FACRSVVINLGCDDDFVI*FSEIRP*EGKILK*I TLNYLCISHQGK*

Frame 1

AVREAELAALQPPRRRSALHSQQWRLRARAAAA APGAAAAAAPPAP*GPRRQATRPSAACRVNAKH *S*ILQIHLSNLQQGLL*IPILQEFKTHT*RD*E HTALSHQEN*RSPLNNTGISLQRT*KTLEKLDE ELMVLSTKWSTNQVGK*WQLKEFGQQWMKKNKN NFLWIWM**CGVVIAHTLFSFMVHSSERVTVGSV WNSCLPRLISFTNMYIVY*MMLFQKKF*AKSL* QL*KH*TT*KKT*KLFTEISNLPIFFWTEVEIL SSVTSASVDSLWTLLPRQEMLAVGHTWHLKE*TQ AHHDKDMMSALMSGVWGSHCMSWPQADFLIQSG IVYLIN*HKS*KEILRS*VILRKGNSPRVSSTL STCALRRMNPKGQSIKSF*NIPLF*CMKNVPLRS HAMFVKSWIKCQLLPALPCMSIDIAATSDSRKK G*EEARRKEFSSRITVFLLLAQTPCAIRLVFVS IMSVYSCHLERASL*YLIDHTVLVLVRETSSCSF VMNIFMKCGSQYDQVVDCD*ITS*IHF*TQNLE MQLLEWCFVRLPNPGRTQ**MYYV*T*KLGLE* EELAQPTRHIAFWSWETKEEFTFFTKCNRLLM*Y SVALQLQLMFGDRCAQPNFLFEISC*IRMNLSL PKTMLRSKR*TLKYRDRTECVLFSFTSPIFQWE DSGVCHLSKKVLILRIFHSQNSVCCQLDVPPATN HQD*KKKTVQKAKFTDVFNSSILSGTTCSSYIF PLGPKPDTLAIITH*QGEVASSNVP*LIR*RFL VGSKRPNLSCLLLAITGPGLQFPNLFPFPCGLLS LCDLRLIQYFAFFLYQKTFTVSRDVPYQGFLAP NLSYSLVFKRLRIVGPSRCGR**RGIFSRDTLD QMRIRNGSLYVHHLLEPLVVHHHFLALEFYWKKI QKAKQNPQHCYKRPFKYLVLLHLPISQVLIRFC LGLPGTEP*ALYDSEAAL*VVQAHWNIKQSWPE MQVMPLQNQIVARIAVSPLRVTVINTVKQ*RENG AV*SHIPVNCRIQK*LSL*STCLISLSSPPRYP KL*TSHCSERRHCSMSAFDHSKPSSSIAPGDSR GICFSAVNFPSGSA*GHFAIMQMEIKAILTVQEL I*PFYCVDDHIRRQF*CINHRLL*TINLRARSL LQCIFIKTKGCIVFTNCENSVRTVHCELWLFFS CTIEKCIKIIKKLMCRDIALFVCKKWSSVT*LLL GALEYFILYSCLNSSSI*DIYMESKCLCNSSIL LPAGQL**I*DVMMTL*FDFLKSDPERGKS*SK LH*IICAFHTREN

Frame 2

PCERPSLLHCSRRGAARLFTPNNGGSEPERRRR LRGRQRQRHPRPRRVPGARPPGRQQHAG*TQST EVEFCKSTFQIYSKVYSESQSYRSSKPTHRETE NTQH*VIRKTEDLP*TTLGFHCRGLERPWRNWTR SLWFCQQNGPQTKWANNGS*KNSVNSG*KRTKT TSYGFGCSNAE**LPIHCSVLWCTLQRG*LLDL YGTHVYLV**VLQICI*CIR*CYSRRNFRQNHFS NCESTKPLKRKLENYSQRYQTFQYSSGQKWKY* AL*LRHQWTACGLYCQDKRCWL*AIHGT*KNRP KRITTRI*CPL*CLEFGDHIV*VGHRPISLSKVE *CI*STNTSRERRSSAAE*F*GKGILPEFHQLC QLVPYEG*IQKAKV*RASETSLYFDV*RTCR*G RMLCL*NPGSNASYSQLSHVCRLISLLHQTLEKR AERKQDVKNFHPVSQCFYCSPRHHVQ*DWCSFP SCLYTPVT*NVHPCNT*LITQC*CWSERPHPAL L**TYS*NVEVSTIKLLTVIRSHLKFISRLKTWR CSYWNGVLSDFQILEGHSDECTMSEHRNSGLSE KSLHSQRDTLPSGAGRQRRNLLSSPSAIDY*CD ILLLYSYS*CLGIDVLSQISCLKYHVKLE*IYLY QKPCCVQRGEH*NIETGQNVFFSPLPVLFFNGK TQESATCQRRC*S*EFFILRIRCAANLMFHLPQ TTRTERRKQYRRQSLQMFLILVFYLEQLVAAIYF PLVPSLIL*PS*LTNREK*LVAMCLD*LDKDF* *AAKDQISVVCFLPSLVQVFSFRISFPSPVVYC RYVTCA*SNILPFFYIKKPLQLAGMFLTKDF*PQ ISHIR*CLKG*E*WGPADVVGDKEASFLETHWT R*GSETAAFTFITC*NLS*SITISWHWNSTGKK YKKQNKTLSTVTRGHLSILCFFTYPLARFSLGFA WASLALNLRLCMTVKQHCEWFKHTGI*NSHGLR CR*CHYRTKSWHVLLCLLSE*QS*ILSNNKGRM VLFKVTSL*IAEFKSDYLFDLLASFPYLLPHGIL NFRLPTVLKGDIALCLPSTTASHHPPLLPGTQE ESVSLLSTSHLAQHRVTLPLCKWR*KQF*LSRS *SDRSIVWMTT*EGNFSVLIIDYYKL*T*GQGVY YNVSLLKQKGV*CSQTVKIV*ELYIVSSGYFSL VP*KNV*KLSKS*CAGILPYLSVKNGAQ*HNCF LELWNILSCILV*IPPLFKIYTWNRSVYVIVLSF CLQVSCNKSRM***LCNLIF*NQTLRGENLKVN YIKLSVHFTPGKM