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Sequences

Sequence CTNNA1.iAug05

GTTGCCTCTAATCCCAGCCACTTGGGAGGCTGAGGCAGGTGAATCTCTTGAACCCGGGAGGCGGAGGTTGCATTGATTCCAAGATCACGCCACTGCACTC CAGCCTGGGTGACAGAGCGAGACTCTGTCTCAAAGGAAAAAAAAAAACAATCATGTCTTACAAAGAAATAGTGAATTGACTCAGTGATAGCAATTGAGCC CCACACACAGTGAAACCAGCCCAGGCCTGCTGTGGGCCAGGCAGCCCAGCGTGTGCACCAGTGAAGCAGAGGCTCGAGACAAATGCTGAGTGATTTTGAC TTTATTCACCATACTGTTCAGAACACTGCACATTAAAATCCAGAATTCTGGGCCTCCGTGCACCTGGCCAAGAGGCTCAGGGTGGCTGGGGGCTGGTGGC CCAGAGATGTGTCTGACCTGTGATCTTTGTCTGGGTGGCAGACCCCTGAGGAGTTGGATGACTCTGACTTTGAGACAGAAGATTTTGATGTCAGAAGCAG GACGAGCGTCCAGACAGAAGACGATCAGCTGATAGCTGGCCAGAGTGCCCGGGCGATCATGGCTCAGCTTCCCCAGGAGCAAAAAGCGAAGATTGCGGAA CAGGTGGCCAGCTTCCAGGAAGAAAAGAGCAAGCTGGATGCTGAAGTGTCCAAATGGGACGACAGTGGCAATGACATCATTGTGCTGGCCAAGCAGATGT GCATGATTATGATGGAGATGACAGACTTTACCCGAGGTAAAGGACCACTCAAAAATACATCGGATGTCATCAGTGCTGCCAAGAAAATTGCTGAGGCAGG ATCCAGGATGGACAAGCTTGGCCGCACCATTGCAGACCATTGCCCCGACTCGGCTTGCAAGCAGGACCTGCTGGCCTACCTGCAACGCATCGCCCTCTAC TGCCACCAGCTGAACATCTGCAGCAAGGTCAAGGCCGAGGTGCAGAATCTCGGCGGGGAGCTTGTTGTCTCTGGGGCAGCCAAGAACTTGATGAATGCTG TGGTGCAGACAGTGAAGGCATCCTACGTCGCCTCTACCAAATACCAAAAGTCACAGGGTATGGCTTCCCTCAACCTTCCTGCTGTGTCATGGAAGATGAA GGCACCAGAGAAAAAGCCATTGGTGAAGAGAGAGAAACAGGATGAGACACAGACCAAGATTAAACGGGCATCTCAGAAGAAGCACGTGAACCCGGTGCAG GCCCTCAGCGAGTTCAAAGCTATGGACAGCATCTAAGTCTGCCCAGGCCGGCCGCCCCCACCCCTCGGGGCTCCTGAATATCAGTCACTGTTCGTCACTC AAATGAATTTGCTAAATACAACACTGATACTAGATTCCACAGGGAAATGGGCAGACTGAACCAGTCCAGGTGGTGAATTTTCCAAGAACATAGTTTAAGT TGATTAAAAATGCTTTTAGAATGCAGGAGCCTACTTCTAGCTGTATTTTTTGTATGCTTAAATAAAAATAAAAATTCATAACCAAAGAGAATCCCACATT AGCTTGTTAGTAATGCTCTGACCAAGCCGAGATGCCCATTCTCTTAGTGATGGCGGCGTTAGGGTTTGAGAGAAGGGAATTTGGCTCAACTTCAGTTGAG AGGGTGCAGTCCAGACAGCTTGACTGCTTTTAAATGACCAAAGATGACCTGTGGTAAGCAACCTGGGCATCTTAGGAAGCAGTCCCTGGAGAAGGCATGT TCCCAGAAAGGTCTCTGGAGGGACAAACTCACTCAGTAAAACATAATGTATCATGAAGAAAACTGATTCTCTATGACATGAAATGAAAATTTTAATGCAT TGTTATAATTACTAATGTACGCTGCTGCAGGACATTAATAAAGTTGCTTTTTTAGGCTACAGTGTCTCGATGCCATAATCAGAACACACTTTTTTTCCTC TTTCTCCCAGCTTCAAATGCAAATTCATCATTGGGCTCACTTCTAATAACTGCAGTGTTTCCCGCCTTGGGCTTGCAGCAGAAAAACCTGACAACATAGT GTTTGCTAAGGCAGTAATTTAGACTTTACCTTATTTGTGATTACTGTAGTGATTGATTGATTGATTACTATTAACTACAAGGTATAATTTACTATCACCT TATTTAAATTTTATGAATTAATTTGAATGTTTTTTACACTAACTAACTTTTCCCAATAAAGTCCACTATGAAACCACGACATCCAAGAGCCCAAAGTCGT CTTCTCTGCCTTCAAGTCATAGATTTGCCCGCAGTATCTGTGGTGCTCTGGGCCCTCCCGGTGTCCGTCTCTTCCAGGATGGGGATGCCCGGGAGGGAAA CTGTCTGTGGCTCTAGGCTGCACGGCTCGTGCCAACCCATCAGGGAGGGCCATGCCCGTTGTCCTATTGAGTGCCCCACCCTGCACCCCCACCTTGGGAA TTCACATGTCCATTCCTTGAGGTTCATGTCAACCTCGGAGGCATCCCTGTCTTCATTATAGCTGACCCCTCTCCTGCGTCCTTCTGTCAGCATATCCCTT CTGCATCCTTCCCGTCACACATACATACCAAGCTATGATGATTGATTGATAGTGGCCTTCGAGATGAAAACCATCCTTAACCCCATGATCCTTCCCAGCT GGCATCCCCACCCTAAGCAAGGTTCCCTAAAGAGAAGCTTGTTGACATTTTCTCCCCTTCCTCACTTACAGTCAGCTGTCACCTTGCTCCCTCCACCTCC CCTCGTCAAGGTGACTGCCTTCCACTGCCAGCCACACTGGCAATTGTCTTGAGACCTCACTGTGCAAGCGGCCTGGGGGCGTGGCGGGGAGGAGGAGGAG GACAAGGACTCCTGAACGCTGCTCACTCGGCTTCCAGGACACCCCGCTCAGGTTCTGCCTGCCTTACTGGGGGCTCCTTCTCCTTTCCTGGGGCTGAGAG GTTGTGTCTGTGCCCACTCTCCTGAGCTCAGCCAGAATCTCGCATGTAAAGACATCCAAATGCCGACTTCTTCTCCCCAGAGCCTGGCTTTTATAACTGA CTGCCGCCTCACGCTCCATTCGGATGTCTAATGAATCTCTCAGACTTGACCTGTCCAAACTGCTTGCCACCTCTACTCGCCACCGCCCACCCCCACACCC TCCATACCGTTTTCCCCACGTCACTGCCCCTCCCCCCCTTCCTCTTGCCAAATGCCTAAAAATAAAAATTCTGAGGTGGATCTGTAGCACAATGGGCTTC TAGTGACAAATCAAAATTCTGATCTTTAAAATGTATGCACAATTTACCTGTTACCGCCTGGCCTAAGCCATCCTGCTGTTACTGCTGTGTAGCAAGTTGC AGCCAAAGCGACCTCACACGGCTCCTGAAGGTCAAGAATCTGGGAGCAGCTCAGCTGGATGGCTGTGACTCGGGGTCTCATGAAGTGGCCAAGATGCCAG CTAAGGCTGCCGTCTCTGAAGGCTTGACCTGGCGAGGATTTGCTTCCAGACTCACTCGCGTGGCTATTGACAGAAGGCCTCAGTTCCATGCCACATGGCC CGTTCCACAGAGCAGCTCACAGCTTGGTAGTAGCTGGCTTCCCCAGAGCGGTATCTGCGAGCAGCGTCCAGATGGAAGCCATGGTGTTCCCTTAAAACCT GATATGGAGGGTGATTCCCCATCATGTCTGTATTCTGTGGGTCACACAGACCAAGGCCGGTCCACTGTGGAAGGCAAGGACGAGGTTGCCAGTGCCCGGC GGTGGGTGGCCACAGCCAGCCTCCCTGCCAGTGTCCCCCACAGACAAGTTGTCCCTGTAAGACAGGTCAGCTCAGGTCATTCCGAGCTCAGACCTTCCAA CGGCGTCCATGTCCCTCAGTAAAAGCCAGAGTCCTAAAGGCCTCTTGCCTGCTCTGGCCTCCTCTCACCATTCTCTGCCATCCTCCATCTGCCCCTGGTG TCCCTGAAGTCTTGAACATGCCGAGCACATCCTGCCTCGTATGCTTCCTGGCCTGGAATGCACTTGCCCCGGGACACCCTGTGCTGGCTTTAGTGTTTCC TTCCTGTCTCCTCAAGTCACCTTATTATCAAAAGGACTCCGACAGGCTAAGTGTGGTGGCTCATGCCTGTAGTCCCAGCACTCGGGAAGGCCGAGGTGGG AGGATCGCTTGAGCCCAGGAGTTCAGGACCTGCCTGGGCAACATGGCAAAACTTTGTCTCTCTTAAAAAAAAAAAAAAAAAAAAAAAAAAG

CTNNA1.F1 (forward primer)
CTNNA1.R1 (reverse primer)
Exon skipping and alternative donor or acceptor

Frame 0

VASNPSHLGG*GR*IS*TREAEVALIPRSRHCT PAWVTERDSVSKEKKKQSCLTKK**IDSVIAIEP HTQ*NQPRPAVGQAAQRVHQ*SRGSRQMLSDFD FIHHTVQNTAH*NPEFWASVHLAKRLRVAGGWW PRDVSDL*SLSGWQTPEELDDSDFETEDFDVRSR TSVQTEDDQLIAGQSARAIMAQLPQEQKAKIAE QVASFQEEKSKLDAEVSKWDDSGNDIIVLAKQM CMIMMEMTDFTRGKGPLKNTSDVISAAKKIAEAG SRMDKLGRTIADHCPDSACKQDLLAYLQRIALY CHQLNICSKVKAEVQNLGGELVVSGAAKNLMNA VVQTVKASYVASTKYQKSQGMASLNLPAVSWKMK APEKKPLVKREKQDETQTKIKRASQKKHVNPVQ ALSEFKAMDSI*VCPGRPPPPLGAPEYQSLFVT QMNLLNTTLILDSTGKWAD*TSPGGEFSKNIV*V D*KCF*NAGAYF*LYFLYA*IKIKIHNQRESHI SLLVML*PSRDAHSLSDGGVRV*EKGIWLNFS* EGAVQTA*LLLNDQR*PVVSNLGILGSSPWRRHV PRKVSGGTNSLSKT*CIMKKTDSL*HEMKILMH CYNY*CTLLQDINKVAFLGYSVSMP*SEHTFFP LSPSFKCKFIIGLTSNNCSVSRLGLAAEKPDNIV FAKAVI*TLPYL*LL**LID*LLLTTRYNLLSP YLNFMN*FECFLH*LTFPNKVHYETTTSKSPKS SSLPSSHRFARSICGALGPPGVRLFQDGDAREGN CLWL*AARLVPTHQGGPCPLSY*VPHPAPPPWE FTCPFLEVHVNLGGIPVFIIADPSPASFCQHIP SASFPSHIHTKL**LIDSGLRDENHP*PHDPSQL ASPP*ARFPKEKLVDIFSPSSLTVSCHLAPSTS PRQGDCLPLPATLAIVLRPHCASGLGAWRGGGG GQGLLNAAHSASRTPRSGSACLTGGSFSFPGAER LCLCPLS*AQPESRM*RHPNADFFSPEPGFYN* LPPHAPFGCLMNLSDLTCPNCLPPLLATAHPHT LHTVFPTSLPLPPFLLPNA*K*KF*GGSVAQWAS SDKSKF*SLKCMHNLPVTAWPKPSCCYCCVASC SQSDLTRLLKVKNLGAAQLDGCDSGSHEVAKMP AKAAVSEGLTWRGFASRLTRVAIDRRPQFHATWP VPQSSSQLGSSWLPQSGICEQRPDGSHGVPLKP DMEGDSPSCLYSVGHTDQGRSTVEGKDEVASAR RWVATASLPASVPHRQVVPVRQVSSGHSELRPSN GVHVPQ*KPES*RPLACSGLLSPFSAILHLPLV SLKS*TCRAHPASYASWPGMHLPRDTLCWL*CF LPVSSSHLIIKRTPTG*VWWLMPVVPALGKAEVG GSLEPRSSGPAWATWQNFVSLKKKKKKKKK

Frame 1

LPLIPATWEAEAGESLEPGRRRLH*FQDHATAL QPG*QSETLSQRKKKNNHVLQRNSELTQ**QLS PTHSETSPGLLWARQPSVCTSEAEARDKC*VILT LFTILFRTLHIKIQNSGPPCTWPRGSGWLGAGG PEMCLTCDLCLGGRPLRSWMTLTLRQKILMSEA GRASRQKTIS**LARVPGRSWLSFPRSKKRRLRN RWPASRKKRASWMLKCPNGTTVAMTSLCWPSRC A*L*WR*QTLPEVKDHSKIHRMSSVLPRKLLRQ DPGWTSLAAPLQTIAPTRLASRTCWPTCNASPST ATS*TSAARSRPRCRISAGSLLSLGQPRT**ML WCRQ*RHPTSPLPNTKSHRVWLPSTFLLCHGR* RHQRKSHW*RERNRMRHRPRLNGHLRRST*TRCR PSASSKLWTASKSAQAGRPHPSGLLNISHCSSL K*IC*IQH*Y*IPQGNGQTEPVQVVNFPRT*FK LIKNAFRMQEPTSSCIFCMLK*K*KFITKENPTL AC**CSDQAEMPILLVMAALGFERREFGSTSVE RVQSRQLDCF*MTKDDLW*ATWAS*EAVPGEGM FPERSLEGQTHSVKHNVS*RKLILYDMK*KF*CI VIITNVRCCRTLIKLLF*ATVSRCHNQNTLFFL FLPASNANSSLGSLLITAVFPALGLQQKNLTT* CLLRQ*FRLYLICDYCSD*LIDYY*LQGIIYYHL I*IL*INLNVFYTN*LFPIKSTMKPRHPRAQSR LLCLQVIDLPAVSVVLWALPVSVSSRMGMPGRE TVCGSRLHGSCQPIREGHARCPIECPTLHPHLGN SHVHSLRFMSTSEASLSSL*LTPLLRPSVSISL LHPSRHTYIPSYDD*LIVAFEMKTILNPMILPS WHPHPKQGSLKRSLLTFSPLPHLQSAVTLLPPPP LVKVTAFHCQPHWQLS*DLTVQAAWGRGGEEEE DKDS*TLLTRLPGHPAQVLPALLGAPSPFLGLR GCVCAHSPELSQNLACKDIQMPTSSPQSLAFITD CRLTLHSDV**ISQT*PVQTACHLYSPPPTPTP SIPFSPRHCPSPPSSCQMPKNKNSEVDL*HNGL LVTNQNSDL*NVCTIYLLPPGLSHPAVTAV*QVA AKATSHGS*RSRIWEQLSWMAVTRGLMKWPRCQ LRLPSLKA*PGEDLLPDSLAWLLTEGLSSMPHG PFHRAAHSLVVAGFPRAVSASSVQMEAMVFP*NL IWRVIPHHVCILWVTQTKAGPLWKARTRLPVPG GGWPQPASLPVSPTDKLSL*DRSAQVIPSSDLP TASMSLSKSQSPKGLLPALASSHHSLPSSICPWC P*SLEHAEHILPRMLPGLECTCPGTPCAGFSVS FLSPQVTLLSKGLRQAKCGGSCL*SQHSGRPRW EDRLSPGVQDLPGQHGKTLSLLKKKKKKKK

Frame 2

CL*SQPLGRLRQVNLLNPGGGGCIDSKITPLHS SLGDRARLCLKGKKKTIMSYKEIVN*LSDSN*A PHTVKPAQACCGPGSPACAPVKQRLETNAE*F* LYSPYCSEHCTLKSRILGLRAPGQEAQGGWGLVA QRCV*PVIFVWVADP*GVG*L*L*DRRF*CQKQ DERPDRRRSADSWPECPGDHGSASPGAKSEDCG TGGQLPGRKEQAGC*SVQMGRQWQ*HHCAGQADV HDYDGDDRLYPR*RTTQKYIGCHQCCQENC*GR IQDGQAWPHHCRPLPRLGLQAGPAGLPATHRPL LPPAEHLQQGQGRGAESRRGACCLWGSQELDECC GADSEGILRRLYQIPKVTGYGFPQPSCCVMEDE GTREKAIGEERETG*DTDQD*TGISEEAREPGA GPQRVQSYGQHLSLPRPAAPTPRGS*ISVTVRHS NEFAKYNTDTRFHREMGRLNQSRW*IFQEHSLS *LKMLLECRSLLLAVFFVCLNKNKNS*PKRIPH *LVSNALTKPRCPFS**WRR*GLREGNLAQLQLR GCSPDSLTAFK*PKMTCGKQPGHLRKQSLEKAC SQKGLWRDKLTQ*NIMYHEEN*FSMT*NENFNA LL*LLMYAAAGH**SCFFRLQCLDAIIRTHFFSS FSQLQMQIHHWAHF**LQCFPPWACSRKT*QHS VC*GSNLDFTLFVITVVID*LITINYKV*FTIT LFKFYELI*MFFTLTNFSQ*SPL*NHDIQEPKVV FSAFKS*ICPQYLWCSGPSRCPSLPGWGCPGGK LSVALGCTARANPSGRAMPVVLLSAPPCTPTLG IHMSIP*GSCQPRRHPCLHYS*PLSCVLLSAYPF CILPVTHTYQAMMID**WPSR*KPSLTP*SFPA GIPTLSKVP*REAC*HFLPFLTYSQLSPCSLHL PSSR*LPSTASHTGNCLETSLCKRPGGVAGRRRR TRTPERCSLGFQDTPLRFCLPYWGLLLLSWG*E VVSVPTLLSSARISHVKTSKCRLLLPRAWLL*L TAASRSIRMSNESLRLDLSKLLATSTRHRPPPHP PYRFPHVTAPPPLPLAKCLKIKILRWICSTMGF **QIKILIFKMYAQFTCYRLA*AILLLLLCSKL QPKRPHTAPEGQESGSSSAGWL*LGVS*SGQDAS *GCRL*RLDLARICFQTHSRGY*QKASVPCHMA RSTEQLTAW**LASPERYLRAASRWKPWCSLKT *YGG*FPIMSVFCGSHRPRPVHCGRQGRGCQCPA VGGHSQPPCQCPPQTSCPCKTGQLRSFRAQTFQ RRPCPSVKARVLKASCLLWPPLTILCHPPSAPG VPEVLNMPSTSCLVCFLAWNALAPGHPVLALVFP SCLLKSPYYQKDSDRLSVVAHACSPSTREGRGG RIA*AQEFRTCLGNMAKLCLS*KKKKKKKK

Sequence CTNNA1.bAug05

GGATACCCCAAACCAAGACAGATGTCTCACAAAACACAAGCAGAGATGTCAGTCTTCAGGCCTCGCATGGAGAAGATGAAAGAGAGGGGTGGCTTTTCCA AAGCCCAGCGTCAGCACGAAGCTTCCACGAGATGATTTTCCCTCTTATTTTGAAGTCCTGAAATGACTGCTGTCCATGCAGGCAACATAAACTTCAAGTG GGATCCTAAAAGTCTAGAGATCAGGACTCTGGCAGTTGAGAGACTGTTGGAGCCTCTTGTTACACAGGTTACAACCCTTGTAAACACCAATAGTAAAGGG CCCTCTAATAAGAAGAGAGGTCGTTCTAAGAAGGCCCATGTTTTGGCTGCATCTGTTGAACAAGCAACTGAGAATTTCTTGGAGAAGGGGGATAAAATTG CGAAGGAGAGCCAGTTTCTCAAGGAGGAGCTTGTGGCTGCTGTAGAAGATGTTCGAAAACAAGGTGATTTGATGAAGGCTGCTGCAGGAGAGTTCGCAGA TGATCCCTGCTCTTCTGTGAAGCGAGGCAACATGGTTCGGGCAGCTCGAGCTTTGCTCTCTGCTGTTACCCGGTTGCTGATTTTGGCTGACATGGCAGAT GTCTACAAATTACTTGTTCAGCTGAAAGTTGTGGAAGATGGTATCTTGAAGTTGAGGAATGCTGGCAATGAACAAGACTTAGGAATCCAGTATAAAGCCC TAAAACCTGAAGTGGATAAGCTGAACATTATGGCAGCCAAAAGACAACAGGAATTGAAAGATGTTGGCCATCGTGATCAGATGGCTGCAGCTAGAGGAAT CCTGCAGAAGAACGTTCCGATCCTCTATACTGCATCCCAGGCATGCCTACAGCACCCTGATGTCGCAGCCTATAAGGCCAACAGGGACCTGATATACAAG CAGCTGCAGCAGGCGGTCACAGGCATTTCCAATGCAGCCCAGGCCACTGCCTCAGACGATGCCTCACAGCACCAGGGTGGAGGAGGAGGAGAACTGGCAT ATGCACTCAATAACTTTGACAAACAAATCATTGTGGACCCCTTGAGCTTCAGCGAGGAGCGCTTTAGGCCTTCCCTGGAGGAGCGTCTGGAAAGCATCAT TAGTGGGGCTGCCTTGATGGCCGACTCGTCCTGCACGCGTGATGACCGTCGTGAGCGAATTGTGGCAGAGTGTAATGCTGTCCGCCAGGCCCTGCAGGAC CTGCTTTCGGAGTACATGGGCAATGCTGGACGTAAAGAAAGAAGTGATGCACTCAATTCTGCAATAGATAAAATGACCAAGAAGACCAGGGACTTGCGTA GACAGCTCCGCAAAGCTGTCATGGACCACGTTTCAGATTCTTTCCTGGAAACCAATGTTCCACTTTTGGTATTGATTGAAGCTGCAAAGAATGGAAATGA GAAAGAAGTTAAGGAGTATGCCCAAGTTTTCCGTGAACATGCCAACAAATTGATTGAGGTTGCCAACTTGGCCTGTTCCATCTCAAATAATGAAGAAGGT GTAAAGCTTGTTCGAATGTCTGCAAGCCAGTTAGAAGCCCTCTGTCCTCAGGTTATTAATGCTGCACTGGCTTTAGCAGCAAAACCACAGAGTAAACTGG CCCAAGAGAACATGGATCTTTTTAAAGAACAATGGGAAAAACAAGTCCGTGTTCTCACAGATGCTGTCGATGACATTACTTCCATTGATGACTTCTTGGC TGTCTCAGAGAATCACATTTTGGAAGATGTGAACAAATGTGTCATTGCTCTCCAAGAGAAGGATGTGGATGGCCTGGACCGCACAGCTGGTGCAATTCGA GGCCGGGCAGCCCGGGTCATTCACGTAGTCACCTCAGAGATGGACAACTATGAGCCAGGAGTCTACACAGAGAAGGTTCTGGAAGCCACTAAGCTGCTCT CCAACACAGTCATGCCACGTTTTACTGAGCAAGTAGAAGCAGCCGTGGAAGCCCTCAGCTCGGACCCTGCCCAGCCCATGGATGAGAATGAGTTTATCGA TGCTTCCCGCCTGGTATATGATGGCATCCGGGACATCAGGAAAGCAGTGCTGATGATAAGGACCCCTGAGGAGTTGGATGACTCTGACTTTGAGACAGAA GATTTTGATGTCAGAAGCAGGACGAGCGTCCAGACAGAAGACGATCAGCTGATAGCTGGCCAGAGTGCCCGGGCGATCATGGCTCAGCTTCCCCAGGAGC AAAAAGCGAAGATTGCGGAACAGGTGGCCAGCTTCCAGGAAGAAAAGAGCAAGCTGGATGCTGAAGTGTCCAAATGGGACGACAGTGGCAATGACATCAT TGTGCTGGCCAAGCAGATGTGCATGATTATGATGGAGATGACAGACTTTACCCGAGGTAAAGGACCACTCAAAAATACATCGGATGTCATCAGTGCTGCC AAGAAAATTGCTGAGGCAGGATCCAGGATGGACAAGCTTGGCCGCACCATTGCAGACCATTGCCCCGACTCGGCTTGCAAGCAGGACCTGCTGGCCTACC TGCAACGCATCGCCCTCTACTGCCACCAGCTGAACATCTGCAGCAAGGTCAAGGCCGAGGTGCAGAATCTCGGCGGGGAGCTTGTTGTCTCTGGGAACTG TGACACCTGCGGGGCACTGCAAGGGCTGAAAGGCTGGCCTCCTCCCCTTTGCTGGCCACTCACTGGGTGGACAGCGCCATGTCCCTGATCCAGGCAGCCA AGAACTTGATGAATGCTGTGGTGCAGACAGTGAAGGCATCCTACGTCGCCTCTACCAAATACCAAAAGTCACAGGGTATGGCTTCCCTCAACCTTCCTGC TGTGTCATGGAAGATGAAGGCACCAGAGAAAAAGCCATTGGTGAAGAGAGAGAAACAGGATGAGACACAGACCAAGATTAAACGGGCATCTCAGAAGAAG CACGTGAACCCGGTGCAGGCCCTCAGCGAGTTCAAAGCTATGGACAGCATCTAAGTCTGCCCAGGCCGGCCGCCCCCACCCCTCGGGGCTCCTGAATATC AGTCACTGTTCGTCACTCAAATGAATTTGCTAAATACAACACTGATACTAGATTCCACAGGGAAATGGGCAGACTGAACCAGTCCAGGTGGTGAATTTTC CAAGAACATAGTTTAAGTTGATTAAAAATGCTTTTAGAATGCAGGAGCCTACTTCTAGCTGTATTTTTTGTATGCTTAAATAAAAATAAAAATTCATAAC CAAAGAGAATCCCACATTAGCTTGTTAGTAATGCTCTGACCAAGCCGAGATGCCCATTCTCTTAGTGATGGCGGCGTTAGGGTTTGAGAGAAGGGAATTT GGCTCAACTTCAGTTGAGAGGGTGCAGTCCAGACAGCTTGACTGCTTTTAAATGACCAAAGATGACCTGTGGTAAGCAACCTGGGCATCTTAGGAAGCAG TCCCTGGAGAAGGCATGTTCCCAGAAAGGTCTCTGGAGGGACAAACTCACTCAGTAAAACATAATGTATCATGAAGAAAACTGATTCTCTATGACATGAA ATGAAAATTTTAATGCATTGTTATAATTACTAATGTACGCTGCTGCAGGACATTAATAAAGTTGCTTTTTTAGGCTACAGTGTCTCGATGCCATAATCAG AACACACTTTTTTTCCTCTTTCTCCCAGCTTCAAATGCAAATTCATCATTGGGCTCACTTCTAATAACTGCAGTGTTTCCCGCCTTGGGCTTGCAGCAGA AAAACCTGACAACATAGTGTTTGCTAAGGCAGTAATTTAGACTTTACCTTATTTGTGATTACTGTAGTGATTGATTGATTGATTACTATTAACTACAAGG TATAATTTACTATCACCTTATTTAAATTTTATGAATTAATTTGAATGTTTTTTACACTAACTAACTTTTCCCAATAAAGTCCACTATGAAACCACGACAT CCAAGAGCCCAAAGTCGTCTTCTCTGCCTTCAAGTCATAGATTTGCCCGCAGTATCTGTGGTGCTCTGGGCCCTCCCGGTGTCCGTCTCTTCCAGGATGG GGATGCCCGGGAGGGAAACTGTCTGTGGCTCTAGGCTGCACGGCTCGTGCCAACCCATCAGGGAGGGCCATGCCCGTTGTCCTATTGAGTGCCCCACCCT GCACCCCCACCTTGGGAATTCACATGTCCATTCCTTGAGGTTCATGTCAACCTCGGAGGCATCCCTGTCTTCATTATAGCTGACCCCTCTCCTGCGTCCT TCTGTCAGCATATCCCTTCTGCATCCTTCCCGTCACACATACATACCAAGCTATGATGATTGATTGATAGTGGCCTTCGAGATGAAAACCATCCTTAACC CCATGATCCTTCCCAGCTGGCATCCCCACCCTAAGCAAGGTTCCCTAAAGAGAAGCTTGTTGACATTTTCTCCCCTTCCTCACTTACAGTCAGCTGTCAC CTTGCTCCCTCCACCTCCCCTCGTCAAGGTGACTGCCTTCCACTGCCAGCCACACTGGCAATTGTCTTGAGACCTCACTGTGCAAGCGGCCTGGGGGCGT GGCGGGGAGGAGGAGGAGGACAAGGACTCCTGAACGCTGCTCACTCGGCTTCCAGGACACCCCGCTCAGGTTCTGCCTGCCTTACTGGGGGCTCCTTCTC CTTTCCTGGGGCTGAGAGGTTGTGTCTGTGCCCACTCTCCTGAGCTCAGCCAGAATCTCGCATGTAAAGACATCCAAATGCCGACTTCTTCTCCCCAGAG CCTGGCTTTTATAACTGACTGCCGCCTCACGCTCCATTCGGATGTCTAATGAATCTCTCAGACTTGACCTGTCCAAACTGCTTGCCACCTCTACTCGCCA CCGCCCACCCCCACACCCTCCATACCGTTTTCCCCACGTCACTGCCCCTCCCCCCCTTCCTCTTGCCAAATGCCTAAAAATAAAAATTCTGAGGTGGATC TGTAGCACAATGGGCTTCTAGTGACAAATCAAAATTCTGATCTTTAAAATGTATGCACAATTTACCTGTTACCGCCTGGCCTAAGCCATCCTGCTGTTAC TGCTGTGTAGCAAGTTGCAGCCAAAGCGACCTCACACGGCTCCTGAAGGTCAAGAATCTGGGAGCAGCTCAGCTGGATGGCTGTGACTCGGGGTCTCATG AAGTGGCCAAGATGCCAGCTAAGGCTGCCGTCTCTGAAGGCTTGACCTGGCGAGGATTTGCTTCCAGACTCACTCGCGTGGCTATTGACAGAAGGCCTCA GTTCCATGCCACATGGCCCGTTCCACAGAGCAGCTCACAGCTTGGTAGTAGCTGGCTTCCCCAGAGCGGTATCTGCGAGCAGCGTCCAGATGGAAGCCAT GGTGTTCCCTTAAAACCTGATATGGAGGGTGATTCCCCATCATGTCTGTATTCTGTGGGTCACACAGACCAAGGCCGGTCCACTGTGGAAGGCAAGGACG AGGTTGCCAGTGCCCGGCGGTGGGTGGCCACAGCCAGCCTCCCTGCCAGTGTCCCCCACAGACAAGTTGTCCCTGTAAGACAGGTCAGCTCAGGTCATTC CGAGCTCAGACCTTCCAACGGCGTCCATGTCCCTCAGTAAAAGCCAGAGTCCTAAAGGCCTCTTGCCTGCTCTGGCCTCCTCTCACCATTCTCTGCCATC CTCCATCTGCCCCTGGTGTCCCTGAAGTCTTGAACATGCCGAGCACATCCTGCCTCGTATGCTTCCTGGCCTGGAATGCACTTGCCCCGGGACACCCTGT GCTGGCTTTAGTGTTTCCTTCCTGTCTCCTCAAGTCACCTTATTATCAAAAGGACTCCGACAGGCTAAGTGTGGTGGCTCATGCCTGTAGTCCCAGCACT CGGGAAGGCCGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCAGGACCTGCCTGGGCAACATGGCAAAACTTTGTCTCTCTTAAAAAAAAAAAAAAAAAA AAAAAAAAG

CTNNA1.F1 (forward primer)
CTNNA1.R1 (reverse primer)
Exon skipping and alternative donor or acceptor

Frame 0

GYPKPRQMSHKTQAEMSVFRPRMEKMKERGGFS KAQRQHEASTR*FSLLF*SPEMTAVHAGNINFKW DPKSLEIRTLAVERLLEPLVTQVTTLVNTNSKG PSNKKRGRSKKAHVLAASVEQATENFLEKGDKI AKESQFLKEELVAAVEDVRKQGDLMKAAAGEFAD DPCSSVKRGNMVRAARALLSAVTRLLILADMAD VYKLLVQLKVVEDGILKLRNAGNEQDLGIQYKA LKPEVDKLNIMAAKRQQELKDVGHRDQMAAARGI LQKNVPILYTASQACLQHPDVAAYKANRDLIYK QLQQAVTGISNAAQATASDDASQHQGGGGGELA YALNNFDKQIIVDPLSFSEERFRPSLEERLESII SGAALMADSSCTRDDRRERIVAECNAVRQALQD LLSEYMGNAGRKERSDALNSAIDKMTKKTRDLR RQLRKAVMDHVSDSFLETNVPLLVLIEAAKNGNE KEVKEYAQVFREHANKLIEVANLACSISNNEEG VKLVRMSASQLEALCPQVINAALALAAKPQSKL AQENMDLFKEQWEKQVRVLTDAVDDITSIDDFLA VSENHILEDVNKCVIALQEKDVDGLDRTAGAIR GRAARVIHVVTSEMDNYEPGVYTEKVLEATKLL SNTVMPRFTEQVEAAVEALSSDPAQPMDENEFID ASRLVYDGIRDIRKAVLMIRTPEELDDSDFETE DFDVRSRTSVQTEDDQLIAGQSARAIMAQLPQE QKAKIAEQVASFQEEKSKLDAEVSKWDDSGNDII VLAKQMCMIMMEMTDFTRGKGPLKNTSDVISAA KKIAEAGSRMDKLGRTIADHCPDSACKQDLLAY LQRIALYCHQLNICSKVKAEVQNLGGELVVSGNC DTCGALQGLKGWPPPLCWPLTGWTAPCP*SRQP RT**MLWCRQ*RHPTSPLPNTKSHRVWLPSTFL LCHGR*RHQRKSHW*RERNRMRHRPRLNGHLRRS T*TRCRPSASSKLWTASKSAQAGRPHPSGLLNI SHCSSLK*IC*IQH*Y*IPQGNGQTEPVQVVNF PRT*FKLIKNAFRMQEPTSSCIFCMLK*K*KFIT KENPTLAC**CSDQAEMPILLVMAALGFERREF GSTSVERVQSRQLDCF*MTKDDLW*ATWAS*EA VPGEGMFPERSLEGQTHSVKHNVS*RKLILYDMK *KF*CIVIITNVRCCRTLIKLLF*ATVSRCHNQ NTLFFLFLPASNANSSLGSLLITAVFPALGLQQ KNLTT*CLLRQ*FRLYLICDYCSD*LIDYY*LQG IIYYHLI*IL*INLNVFYTN*LFPIKSTMKPRH PRAQSRLLCLQVIDLPAVSVVLWALPVSVSSRM GMPGRETVCGSRLHGSCQPIREGHARCPIECPTL HPHLGNSHVHSLRFMSTSEASLSSL*LTPLLRP SVSISLLHPSRHTYIPSYDD*LIVAFEMKTILN PMILPSWHPHPKQGSLKRSLLTFSPLPHLQSAVT LLPPPPLVKVTAFHCQPHWQLS*DLTVQAAWGR GGEEEEDKDS*TLLTRLPGHPAQVLPALLGAPS PFLGLRGCVCAHSPELSQNLACKDIQMPTSSPQS LAFITDCRLTLHSDV**ISQT*PVQTACHLYSP PPTPTPSIPFSPRHCPSPPSSCQMPKNKNSEVD L*HNGLLVTNQNSDL*NVCTIYLLPPGLSHPAVT AV*QVAAKATSHGS*RSRIWEQLSWMAVTRGLM KWPRCQLRLPSLKA*PGEDLLPDSLAWLLTEGL SSMPHGPFHRAAHSLVVAGFPRAVSASSVQMEAM VFP*NLIWRVIPHHVCILWVTQTKAGPLWKART RLPVPGGGWPQPASLPVSPTDKLSL*DRSAQVI PSSDLPTASMSLSKSQSPKGLLPALASSHHSLPS SICPWCP*SLEHAEHILPRMLPGLECTCPGTPC AGFSVSFLSPQVTLLSKGLRQAKCGGSCL*SQH SGRPRWEDRLSPGVQDLPGQHGKTLSLLKKKKKK KK

Frame 1

DTPNQDRCLTKHKQRCQSSGLAWRR*KRGVAFP KPSVSTKLPRDDFPSYFEVLK*LLSMQAT*TSS GILKV*RSGLWQLRDCWSLLLHRLQPL*TPIVKG PLIRREVVLRRPMFWLHLLNKQLRISWRRGIKL RRRASFSRRSLWLL*KMFENKVI**RLLQESSQ MIPALL*SEATWFGQLELCSLLLPGC*FWLTWQM STNYLFS*KLWKMVS*S*GMLAMNKT*ESSIKP *NLKWIS*TLWQPKDNRN*KMLAIVIRWLQLEE SCRRTFRSSILHPRHAYSTLMSQPIRPTGT*YTS SCSRRSQAFPMQPRPLPQTMPHSTRVEEEENWH MHSITLTNKSLWTP*ASARSALGLPWRSVWKAS LVGLP*WPTRPARVMTVVSELWQSVMLSARPCRT CFRSTWAMLDVKKEVMHSILQ*IK*PRRPGTCV DSSAKLSWTTFQILSWKPMFHFWY*LKLQRMEM RKKLRSMPKFSVNMPTN*LRLPTWPVPSQIMKKV *SLFECLQAS*KPSVLRLLMLHWL*QQNHRVNW PKRTWIFLKNNGKNKSVFSQMLSMTLLPLMTSW LSQRITFWKM*TNVSLLSKRRMWMAWTAQLVQFE AGQPGSFT*SPQRWTTMSQESTQRRFWKPLSCS PTQSCHVLLSK*KQPWKPSARTLPSPWMRMSLS MLPAWYMMASGTSGKQC***GPLRSWMTLTLRQK ILMSEAGRASRQKTIS**LARVPGRSWLSFPRS KKRRLRNRWPASRKKRASWMLKCPNGTTVAMTS LCWPSRCA*L*WR*QTLPEVKDHSKIHRMSSVLP RKLLRQDPGWTSLAAPLQTIAPTRLASRTCWPT CNASPSTATS*TSAARSRPRCRISAGSLLSLGT VTPAGHCKG*KAGLLPFAGHSLGGQRHVPDPGSQ ELDECCGADSEGILRRLYQIPKVTGYGFPQPSC CVMEDEGTREKAIGEERETG*DTDQD*TGISEE AREPGAGPQRVQSYGQHLSLPRPAAPTPRGS*IS VTVRHSNEFAKYNTDTRFHREMGRLNQSRW*IF QEHSLS*LKMLLECRSLLLAVFFVCLNKNKNS* PKRIPH*LVSNALTKPRCPFS**WRR*GLREGNL AQLQLRGCSPDSLTAFK*PKMTCGKQPGHLRKQ SLEKACSQKGLWRDKLTQ*NIMYHEEN*FSMT* NENFNALL*LLMYAAAGH**SCFFRLQCLDAIIR THFFSSFSQLQMQIHHWAHF**LQCFPPWACSR KT*QHSVC*GSNLDFTLFVITVVID*LITINYK V*FTITLFKFYELI*MFFTLTNFSQ*SPL*NHDI QEPKVVFSAFKS*ICPQYLWCSGPSRCPSLPGW GCPGGKLSVALGCTARANPSGRAMPVVLLSAPP CTPTLGIHMSIP*GSCQPRRHPCLHYS*PLSCVL LSAYPFCILPVTHTYQAMMID**WPSR*KPSLT P*SFPAGIPTLSKVP*REAC*HFLPFLTYSQLS PCSLHLPSSR*LPSTASHTGNCLETSLCKRPGGV AGRRRRTRTPERCSLGFQDTPLRFCLPYWGLLL LSWG*EVVSVPTLLSSARISHVKTSKCRLLLPR AWLL*LTAASRSIRMSNESLRLDLSKLLATSTRH RPPPHPPYRFPHVTAPPPLPLAKCLKIKILRWI CSTMGF**QIKILIFKMYAQFTCYRLA*AILLL LLCSKLQPKRPHTAPEGQESGSSSAGWL*LGVS* SGQDAS*GCRL*RLDLARICFQTHSRGY*QKAS VPCHMARSTEQLTAW**LASPERYLRAASRWKP WCSLKT*YGG*FPIMSVFCGSHRPRPVHCGRQGR GCQCPAVGGHSQPPCQCPPQTSCPCKTGQLRSF RAQTFQRRPCPSVKARVLKASCLLWPPLTILCH PPSAPGVPEVLNMPSTSCLVCFLAWNALAPGHPV LALVFPSCLLKSPYYQKDSDRLSVVAHACSPST REGRGGRIA*AQEFRTCLGNMAKLCLS*KKKKK KKK

Frame 2

IPQTKTDVSQNTSRDVSLQASHGEDEREGWLFQ SPASARSFHEMIFPLILKS*NDCCPCRQHKLQV GS*KSRDQDSGS*ETVGASCYTGYNPCKHQ**R AL**EERSF*EGPCFGCIC*TSN*EFLGEGG*NC EGEPVSQGGACGCCRRCSKTR*FDEGCCRRVRR *SLLFCEARQHGSGSSSFALCCYPVADFG*HGR CLQITCSAESCGRWYLEVEECWQ*TRLRNPV*SP KT*SG*AEHYGSQKTTGIERCWPS*SDGCS*RN PAEERSDPLYCIPGMPTAP*CRSL*GQQGPDIQ AAAAGGHRHFQCSPGHCLRRCLTAPGWRRRRTGI CTQ*L*QTNHCGPLELQRGAL*AFPGGASGKHH *WGCLDGRLVLHA**PS*ANCGRV*CCPPGPAG PAFGVHGQCWT*RKK*CTQFCNR*NDQEDQGLA* TAPQSCHGPRFRFFPGNQCSTFGID*SCKEWK* ERS*GVCPSFP*TCQQID*GCQLGLFHLK**RR CKACSNVCKPVRSPLSSGY*CCTGFSSKTTE*TG PREHGSF*RTMGKTSPCSHRCCR*HYFH**LLG CLRESHFGRCEQMCHCSPREGCGWPGPHSWCNS RPGSPGHSRSHLRDGQL*ARSLHREGSGSH*AAL QHSHATFY*ASRSSRGSPQLGPCPAHG*E*VYR CFPPGI*WHPGHQESSADDKDP*GVG*L*L*DR RF*CQKQDERPDRRRSADSWPECPGDHGSASPGA KSEDCGTGGQLPGRKEQAGC*SVQMGRQWQ*HH CAGQADVHDYDGDDRLYPR*RTTQKYIGCHQCC QENC*GRIQDGQAWPHHCRPLPRLGLQAGPAGLP ATHRPLLPPAEHLQQGQGRGAESRRGACCLWEL *HLRGTARAERLASSPLLATHWVDSAMSLIQAA KNLMNAVVQTVKASYVASTKYQKSQGMASLNLPA VSWKMKAPEKKPLVKREKQDETQTKIKRASQKK HVNPVQALSEFKAMDSI*VCPGRPPPPLGAPEY QSLFVTQMNLLNTTLILDSTGKWAD*TSPGGEFS KNIV*VD*KCF*NAGAYF*LYFLYA*IKIKIHN QRESHISLLVML*PSRDAHSLSDGGVRV*EKGI WLNFS*EGAVQTA*LLLNDQR*PVVSNLGILGSS PWRRHVPRKVSGGTNSLSKT*CIMKKTDSL*HE MKILMHCYNY*CTLLQDINKVAFLGYSVSMP*S EHTFFPLSPSFKCKFIIGLTSNNCSVSRLGLAAE KPDNIVFAKAVI*TLPYL*LL**LID*LLLTTR YNLLSPYLNFMN*FECFLH*LTFPNKVHYETTT SKSPKSSSLPSSHRFARSICGALGPPGVRLFQDG DAREGNCLWL*AARLVPTHQGGPCPLSY*VPHP APPPWEFTCPFLEVHVNLGGIPVFIIADPSPAS FCQHIPSASFPSHIHTKL**LIDSGLRDENHP*P HDPSQLASPP*ARFPKEKLVDIFSPSSLTVSCH LAPSTSPRQGDCLPLPATLAIVLRPHCASGLGA WRGGGGGQGLLNAAHSASRTPRSGSACLTGGSFS FPGAERLCLCPLS*AQPESRM*RHPNADFFSPE PGFYN*LPPHAPFGCLMNLSDLTCPNCLPPLLA TAHPHTLHTVFPTSLPLPPFLLPNA*K*KF*GGS VAQWASSDKSKF*SLKCMHNLPVTAWPKPSCCY CCVASCSQSDLTRLLKVKNLGAAQLDGCDSGSH EVAKMPAKAAVSEGLTWRGFASRLTRVAIDRRPQ FHATWPVPQSSSQLGSSWLPQSGICEQRPDGSH GVPLKPDMEGDSPSCLYSVGHTDQGRSTVEGKD EVASARRWVATASLPASVPHRQVVPVRQVSSGHS ELRPSNGVHVPQ*KPES*RPLACSGLLSPFSAI LHLPLVSLKS*TCRAHPASYASWPGMHLPRDTL CWL*CFLPVSSSHLIIKRTPTG*VWWLMPVVPAL GKAEVGGSLEPRSSGPAWATWQNFVSLKKKKKK KKK