Sequences - Rnomics 📊 Palace

        ☰
    
        Rnomics 📊 Palace
    
                       Back to Rnomics Platform
            
                       Open Session

1 warning:

Access restricted to public data.
Please sign in at Rnomics Platform to access your data.

Navigation

Home

Sequences

Sequence CTNNA1.jAug05

TGGGCACAATGGCAATGACATCATTGTGCTGGCCAAGCAGATGTGCATGATTATGATGGAGATGACAGACTTTACCCGAGGTAAAGGACCACTCAAAAAT ACATCGGATGTCATCAGTGCTGCCAAGAAAATTGCTGAGGCAGGATCCAGGATGGACAAGCTTGGCCGCACCATTGCAGACCATTGCCCCGACTCGGCTT GCAAGCAGGACCTGCTGGCCTACCTGCAACGCATCGCCCTCTACTGCCACCAGCTGAACATCTGCAGCAAGGTCAAGGCCGAGGTGCAGAATCTCGGCGG GGAGCTTGTTGTCTCTGGGGTGGACAGCGCCATGTCCCTGATCCAGGCAGCCAAGAACTTGATGAATGCTGTGGTGCAGACAGTGAAGGCATCCTACGTC GCCTCTACCAAATACCAAAAGTCACAGGGTATGGCTTCCCTCAACCTTCCTGCTGTGTCATGGAAGATGAAGGCACCAGAGAAAAAGCCATTGGTGAAGA GAGAGAAACAGGATGAGACACAGACCAAGATTAAACGGGCATCTCAGAAGAAGCACGTGAACCCGGTGCAGGCCCTCAGCGAGTTCAAAGCTATGGACAG CATCTAAGTCTGCCCAGGCCGGCCGCCCCCACCCCTCGGGGCTCCTGAATATCAGTCACTGTTCGTCACTCAAATGAATTTGCTAAATACAACACTGATA CTAGATTCCACAGGGAAATGGGCAGACTGAACCAGTCCAGGTGGTGAATTTTCCAAGAACATAGTTTAAGTTGATTAAAAATGCTTTTAGAATGCAGGAG CCTACTTCTAGCTGTATTTTTTGTATGCTTAAATAAAAATAAAAATTCATAACCAAAGAGAATCCCACATTAGCTTGTTAGTAATGCTCTGACCAAGCCG AGATGCCCATTCTCTTAGTGATGGCGGCGTTAGGGTTTGAGAGAAGGGAATTTGGCTCAACTTCAGTTGAGAGGGTGCAGTCCAGACAGCTTGACTGCTT TTAAATGACCAAAGATGACCTGTGGTAAGCAACCTGGGCATCTTAGGAAGCAGTCCCTGGAGAAGGCATGTTCCCAGAAAGGTCTCTGGAGGGACAAACT CACTCAGTAAAACATAATGTATCATGAAGAAAACTGATTCTCTATGACATGAAATGAAAATTTTAATGCATTGTTATAATTACTAATGTACGCTGCTGCA GGACATTAATAAAGTTGCTTTTTTAGGCTACAGTGTCTCGATGCCATAATCAGAACACACTTTTTTTCCTCTTTCTCCCAGCTTCAAATGCAAATTCATC ATTGGGCTCACTTCTAATAACTGCAGTGTTTCCCGCCTTGGGCTTGCAGCAGAAAAACCTGACAACATAGTGTTTGCTAAGGCAGTAATTTAGACTTTAC CTTATTTGTGATTACTGTAGTGATTGATTGATTGATTACTATTAACTACAAGGTATAATTTACTATCACCTTATTTAAATTTTATGAATTAATTTGAATG TTTTTTACACTAACTAACTTTTCCCAATAAAGTCCACTATGAAACCACGACATCCAAGAGCCCAAAGTCGTCTTCTCTGCCTTCAAGTCATAGATTTGCC CGCAGTATCTGTGGTGCTCTGGGCCCTCCCGGTGTCCGTCTCTTCCAGGATGGGGATGCCCGGGAGGGAAACTGTCTGTGGCTCTAGGCTGCACGGCTCG TGCCAACCCATCAGGGAGGGCCATGCCCGTTGTCCTATTGAGTGCCCCACCCTGCACCCCCACCTTGGGAATTCACATGTCCATTCCTTGAGGTTCATGT CAACCTCGGAGGCATCCCTGTCTTCATTATAGCTGACCCCTCTCCTGCGTCCTTCTGTCAGCATATCCCTTCTGCATCCTTCCCGTCACACATACATACC AAGCTATGATGATTGATTGATAGTGGCCTTCGAGATGAAAACCATCCTTAACCCCATGATCCTTCCCAGCTGGCATCCCCACCCTAAGCAAGGTTCCCTA AAGAGAAGCTTGTTGACATTTTCTCCCCTTCCTCACTTACAGTCAGCTGTCACCTTGCTCCCTCCACCTCCCCTCGTCAAGGTGACTGCCTTCCACTGCC AGCCACACTGGCAATTGTCTTGAGACCTCACTGTGCAAGCGGCCTGGGGGCGTGGCGGGGAGGAGGAGGAGGACAAGGACTCCTGAACGCTGCTCACTCG GCTTCCAGGACACCCCGCTCAGGTTCTGCCTGCCTTACTGGGGGCTCCTTCTCCTTTCCTGGGGCTGAGAGGTTGTGTCTGTGCCCACTCTCCTGAGCTC AGCCAGAATCTCGCATGTAAAGACATCCAAATGCCGACTTCTTCTCCCCAGAGCCTGGCTTTTATAACTGACTGCCGCCTCACGCTCCATTCGGATGTCT AATGAATCTCTCAGACTTGACCTGTCCAAACTGCTTGCCACCTCTACTCGCCACCGCCCACCCCCACACCCTCCATACCGTTTTCCCCACGTCACTGCCC CTCCCCCCCTTCCTCTTGCCAAATGCCTAAAAATAAAAATTCTGAGGTGGATCTGTAGCACAATGGGCTTCTAGTGACAAATCAAAATTCTGATCTTTAA AATGTATGCACAATTTACCTGTTACCGCCTGGCCTAAGCCATCCTGCTGTTACTGCTGTGTAGCAAGTTGCAGCCAAAGCGACCTCACACGGCTCCTGAA GGTCAAGAATCTGGGAGCAGCTCAGCTGGATGGCTGTGACTCGGGGTCTCATGAAGTGGCCAAGATGCCAGCTAAGGCTGCCGTCTCTGAAGGCTTGACC TGGCGAGGATTTGCTTCCAGACTCACTCGCGTGGCTATTGACAGAAGGCCTCAGTTCCATGCCACATGGCCCGTTCCACAGAGCAGCTCACAGCTTGGTA GTAGCTGGCTTCCCCAGAGCGGTATCTGCGAGCAGCGTCCAGATGGAAGCCATGGTGTTCCCTTAAAACCTGATATGGAGGGTGATTCCCCATCATGTCT GTATTCTGTGGGTCACACAGACCAAGGCCGGTCCACTGTGGAAGGCAAGGACGAGGTTGCCAGTGCCCGGCGGTGGGTGGCCACAGCCAGCCTCCCTGCC AGTGTCCCCCACAGACAAGTTGTCCCTGTAAGACAGGTCAGCTCAGGTCATTCCGAGCTCAGACCTTCCAACGGCGTCCATGTCCCTCAGTAAAAGCCAG AGTCCTAAAGGCCTCTTGCCTGCTCTGGCCTCCTCTCACCATTCTCTGCCATCCTCCATCTGCCCCTGGTGTCCCTGAAGTCTTGAACATGCCGAGCACA TCCTGCCTCGTATGCTTCCTGGCCTGGAATGCACTTGCCCCGGGACACCCTGTGCTGGCTTTAGTGTTTCCTTCCTGTCTCCTCAAGTCACCTTATTATC AAAAGGACTCCGACAGGCTAAGTGTGGTGGCTCATGCCTGTAGTCCCAGCACTCGGGAAGGCCGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCAGGAC CTGCCTGGGCAACATGGCAAAACTTTGTCTCTCTTAAAAAAAAAAAAAAAAAAAAAAAAAAG

CTNNA1.F1 (forward primer)
CTNNA1.R1 (reverse primer)
Single exon skipping

Frame 0

WAQWQ*HHCAGQADVHDYDGDDRLYPR*RTTQK YIGCHQCCQENC*GRIQDGQAWPHHCRPLPRLGL QAGPAGLPATHRPLLPPAEHLQQGQGRGAESRR GACCLWGGQRHVPDPGSQELDECCGADSEGILR RLYQIPKVTGYGFPQPSCCVMEDEGTREKAIGEE RETG*DTDQD*TGISEEAREPGAGPQRVQSYGQ HLSLPRPAAPTPRGS*ISVTVRHSNEFAKYNTD TRFHREMGRLNQSRW*IFQEHSLS*LKMLLECRS LLLAVFFVCLNKNKNS*PKRIPH*LVSNALTKP RCPFS**WRR*GLREGNLAQLQLRGCSPDSLTA FK*PKMTCGKQPGHLRKQSLEKACSQKGLWRDKL TQ*NIMYHEEN*FSMT*NENFNALL*LLMYAAA GH**SCFFRLQCLDAIIRTHFFSSFSQLQMQIH HWAHF**LQCFPPWACSRKT*QHSVC*GSNLDFT LFVITVVID*LITINYKV*FTITLFKFYELI*M FFTLTNFSQ*SPL*NHDIQEPKVVFSAFKS*IC PQYLWCSGPSRCPSLPGWGCPGGKLSVALGCTAR ANPSGRAMPVVLLSAPPCTPTLGIHMSIP*GSC QPRRHPCLHYS*PLSCVLLSAYPFCILPVTHTY QAMMID**WPSR*KPSLTP*SFPAGIPTLSKVP* REAC*HFLPFLTYSQLSPCSLHLPSSR*LPSTA SHTGNCLETSLCKRPGGVAGRRRRTRTPERCSL GFQDTPLRFCLPYWGLLLLSWG*EVVSVPTLLSS ARISHVKTSKCRLLLPRAWLL*LTAASRSIRMS NESLRLDLSKLLATSTRHRPPPHPPYRFPHVTA PPPLPLAKCLKIKILRWICSTMGF**QIKILIFK MYAQFTCYRLA*AILLLLLCSKLQPKRPHTAPE GQESGSSSAGWL*LGVS*SGQDAS*GCRL*RLD LARICFQTHSRGY*QKASVPCHMARSTEQLTAW* *LASPERYLRAASRWKPWCSLKT*YGG*FPIMS VFCGSHRPRPVHCGRQGRGCQCPAVGGHSQPPC QCPPQTSCPCKTGQLRSFRAQTFQRRPCPSVKAR VLKASCLLWPPLTILCHPPSAPGVPEVLNMPST SCLVCFLAWNALAPGHPVLALVFPSCLLKSPYY QKDSDRLSVVAHACSPSTREGRGGRIA*AQEFRT CLGNMAKLCLS*KKKKKKKK

Frame 1

GHNGNDIIVLAKQMCMIMMEMTDFTRGKGPLKN TSDVISAAKKIAEAGSRMDKLGRTIADHCPDSA CKQDLLAYLQRIALYCHQLNICSKVKAEVQNLGG ELVVSGVDSAMSLIQAAKNLMNAVVQTVKASYV ASTKYQKSQGMASLNLPAVSWKMKAPEKKPLVK REKQDETQTKIKRASQKKHVNPVQALSEFKAMDS I*VCPGRPPPPLGAPEYQSLFVTQMNLLNTTLI LDSTGKWAD*TSPGGEFSKNIV*VD*KCF*NAG AYF*LYFLYA*IKIKIHNQRESHISLLVML*PSR DAHSLSDGGVRV*EKGIWLNFS*EGAVQTA*LL LNDQR*PVVSNLGILGSSPWRRHVPRKVSGGTN SLSKT*CIMKKTDSL*HEMKILMHCYNY*CTLLQ DINKVAFLGYSVSMP*SEHTFFPLSPSFKCKFI IGLTSNNCSVSRLGLAAEKPDNIVFAKAVI*TL PYL*LL**LID*LLLTTRYNLLSPYLNFMN*FEC FLH*LTFPNKVHYETTTSKSPKSSSLPSSHRFA RSICGALGPPGVRLFQDGDAREGNCLWL*AARL VPTHQGGPCPLSY*VPHPAPPPWEFTCPFLEVHV NLGGIPVFIIADPSPASFCQHIPSASFPSHIHT KL**LIDSGLRDENHP*PHDPSQLASPP*ARFP KEKLVDIFSPSSLTVSCHLAPSTSPRQGDCLPLP ATLAIVLRPHCASGLGAWRGGGGGQGLLNAAHS ASRTPRSGSACLTGGSFSFPGAERLCLCPLS*A QPESRM*RHPNADFFSPEPGFYN*LPPHAPFGCL MNLSDLTCPNCLPPLLATAHPHTLHTVFPTSLP LPPFLLPNA*K*KF*GGSVAQWASSDKSKF*SL KCMHNLPVTAWPKPSCCYCCVASCSQSDLTRLLK VKNLGAAQLDGCDSGSHEVAKMPAKAAVSEGLT WRGFASRLTRVAIDRRPQFHATWPVPQSSSQLG SSWLPQSGICEQRPDGSHGVPLKPDMEGDSPSCL YSVGHTDQGRSTVEGKDEVASARRWVATASLPA SVPHRQVVPVRQVSSGHSELRPSNGVHVPQ*KP ES*RPLACSGLLSPFSAILHLPLVSLKS*TCRAH PASYASWPGMHLPRDTLCWL*CFLPVSSSHLII KRTPTG*VWWLMPVVPALGKAEVGGSLEPRSSG PAWATWQNFVSLKKKKKKKKK

Frame 2

GTMAMTSLCWPSRCA*L*WR*QTLPEVKDHSKI HRMSSVLPRKLLRQDPGWTSLAAPLQTIAPTRL ASRTCWPTCNASPSTATS*TSAARSRPRCRISA GSLLSLGWTAPCP*SRQPRT**MLWCRQ*RHPTS PLPNTKSHRVWLPSTFLLCHGR*RHQRKSHW*R ERNRMRHRPRLNGHLRRST*TRCRPSASSKLWT ASKSAQAGRPHPSGLLNISHCSSLK*IC*IQH*Y *IPQGNGQTEPVQVVNFPRT*FKLIKNAFRMQE PTSSCIFCMLK*K*KFITKENPTLAC**CSDQA EMPILLVMAALGFERREFGSTSVERVQSRQLDCF *MTKDDLW*ATWAS*EAVPGEGMFPERSLEGQT HSVKHNVS*RKLILYDMK*KF*CIVIITNVRCC RTLIKLLF*ATVSRCHNQNTLFFLFLPASNANSS LGSLLITAVFPALGLQQKNLTT*CLLRQ*FRLY LICDYCSD*LIDYY*LQGIIYYHLI*IL*INLN VFYTN*LFPIKSTMKPRHPRAQSRLLCLQVIDLP AVSVVLWALPVSVSSRMGMPGRETVCGSRLHGS CQPIREGHARCPIECPTLHPHLGNSHVHSLRFM STSEASLSSL*LTPLLRPSVSISLLHPSRHTYIP SYDD*LIVAFEMKTILNPMILPSWHPHPKQGSL KRSLLTFSPLPHLQSAVTLLPPPPLVKVTAFHC QPHWQLS*DLTVQAAWGRGGEEEEDKDS*TLLTR LPGHPAQVLPALLGAPSPFLGLRGCVCAHSPEL SQNLACKDIQMPTSSPQSLAFITDCRLTLHSDV **ISQT*PVQTACHLYSPPPTPTPSIPFSPRHCP SPPSSCQMPKNKNSEVDL*HNGLLVTNQNSDL* NVCTIYLLPPGLSHPAVTAV*QVAAKATSHGS* RSRIWEQLSWMAVTRGLMKWPRCQLRLPSLKA*P GEDLLPDSLAWLLTEGLSSMPHGPFHRAAHSLV VAGFPRAVSASSVQMEAMVFP*NLIWRVIPHHV CILWVTQTKAGPLWKARTRLPVPGGGWPQPASLP VSPTDKLSL*DRSAQVIPSSDLPTASMSLSKSQ SPKGLLPALASSHHSLPSSICPWCP*SLEHAEH ILPRMLPGLECTCPGTPCAGFSVSFLSPQVTLLS KGLRQAKCGGSCL*SQHSGRPRWEDRLSPGVQD LPGQHGKTLSLLKKKKKKKK

Sequence CTNNA1.dAug05

TCTTTTGTTTGGGTTTTCCAGAAGCTTTACTGTTATAAAGCACCATGCTTTGTAACAGCATCGGGGTCGTTGCAGGACCCCCTGATTACAATAAAGCGAG AAAGAAGAACTCCTGACTTAAAAACATGATATTCCTTCAGTGTAGGAAGCAGCAGTGAGGTTGTAATAAAGGCTGCATTCAAGAAGACCCATCTGAACAG CTGGACGTAAAGAAAGAAGTGATGCACTCAATTCTGCAATAGATAAAATGACCAAGAAGACCAGGGACTTGCGTAGACAGCTCCGCAAAGCTGTCATGGA CCACGTTTCAGATTCTTTCCTGGAAACCAATGTTCCACTTTTGGTATTGATTGAAGCTGCAAAGAATGGAAATGAGAAAGAAGTTAAGGAGTATGCCCAA GTTTTCCGTGAACATGCCAACAAATTGATTGAGGTTGCCAACTTGGCCTGTTCCATCTCAAATAATGAAGAAGGTGTAAAGCTTGTTCGAATGTCTGCAA GCCAGTTAGAAGCCCTCTGTCCTCAGGTTATTAATGCTGCACTGGCTTTAGCAGCAAAACCACAGAGTAAACTGGCCCAAGAGAACATGGATCTTTTTAA AGAACAATGGGAAAAACAAGTCCGTGTTCTCACAGATGCTGTCGATGACATTACTTCCATTGATGACTTCTTGGCTGTCTCAGAGAATCACATTTTGGAA GATGTGAACAAATGTGTCATTGCTCTCCAAGAGAAGGATGTGGATGGCCTGGACCGCACAGCTGGTGCAATTCGAGGCCGGGCAGCCCGGGTCATTCACG TAGTCACCTCAGAGATGGACAACTATGAGCCAGGAGTCTACACAGAGAAGGTTCTGGAAGCCACTAAGCTGCTCTCCAACACAGTCATGCCACGTTTTAC TGAGCAAGTAGAAGCAGCCGTGGAAGCCCTCAGCTCGGACCCTGCCCAGCCCATGGATGAGAATGAGTTTATCGATGCTTCCCGCCTGGTATATGATGGC ATCCGGGACATCAGGAAAGCAGTGCTGATGATAAGGACCCCTGAGGAGTTGGATGACTCTGACTTTGAGACAGAAGATTTTGATGTCAGAAGCAGGACGA GCGTCCAGACAGAAGACGATCAGCTGATAGCTGGCCAGAGTGCCCGGGCGATCATGGCTCAGCTTCCCCAGGAGCAAAAAGCGAAGATTGCGGAACAGGT GGCCAGCTTCCAGGAAGAAAAGAGCAAGCTGGATGCTGAAGTGTCCAAATGGGACGACAGTGGCAATGACATCATTGTGCTGGCCAAGCAGATGTGCATG ATTATGATGGAGATGACAGACTTTACCCGAGGTAAAGGACCACTCAAAAATACATCGGATGTCATCAGTGCTGCCAAGAAAATTGCTGAGGCAGGATCCA GGATGGACAAGCTTGGCCGCACCATTGCAGACCATGTAAGTGACAGACTTGCCAGGTGGGTCTCCAAGCTCCTCCTGGGGCTCAGGCAGCCCAGCCTGGT CCATTCAGGGTAAGTTTCATGGGCCACAGCACTTTTGCCACTCATCTCTAAAAATGGTTCTTATTAATCCCAGCATAGATTTGTAAGGATTCTCTAGAGC GGATGTGTATGTCCTGGAATCTTCCATCGCTTGGTAGCCAAAATTAACCTCTGCTATTGTCTCTTATGTCTCAGTTCCCTCTCTCCCCCTCTGGGCAGGG GCCGAGAGCAGCCAGAAGCCATGTGGCCAGTGCTGTGGAAGGCTGGTGTGCCTAGATGGGCCCAGGTTTCTTCATCCTCAGCACTGTGTTTCTCAGCCAC CCCGTCTTCATCCCCCTATTCCCTCACACTGATAGGTACAAAAATACGGTTCCTACCGCCTGGAAATTGTACAATACATTTCATTTGGTGTTTTAAGCCT GATCAAGAGAAGCTCTTTCTCAGTTACAGTTTTTCTCTGTAGTTAACAAGCCTCCCCACCATTTTCCCTGAAATAGCCAAACTCTCAGCCTCATCTAGGC CACAGGATGTGTGAGACACAAGCCTCCTTACACAAGCCAAAGATTGTAAAGGCATCCTTGACTCCCACACAATCGGTAATAGGGCCTATTGCTTCCATTG GCCAAATGTATCTTGACACAAACCAGTCTTGTCCTTTCTGCAGATCTCCATAGCCACTTGGACTACTGTGGCCGCTCCCGATTTCCTGTTCTTTCCTCTC TCCACTTCCTTCTGCATCCAGCTGTCAATACTGTCATCTGAAAATGCAGATCACATGGCTCTGCCCTGCTTCCATGACTACTTAGAATACAGCTCATCTC CAGTATGGTCTGCGGGGTCTCAGTTCATACCACTCTCTGTGGTGTACTTTGCTCCAGCCTCACTGGAAGAAAATGTTTGCTTGCTATAAACACTTGAGAG GTGCTCTGTGTTTCTCCCTTTGGGTAAAGCTTTCTAGGCTGGTCCTAATCTGGGAGCCTCTAAAACTCTGAGATGTGGAGCGAGACAGGAACTGGGCAGG GGCCACCCTTTTCTCTTTGACCATCCCCAACTGCTTTTTTTCTTGCTTTACTTTACTCACTTGTCTTCCACCTCAGCCTGCAGTGAGTGGCTTTTGTCAT CAGGCATTAATAACGCAAAGTGCCTCAGAGCAGAAGAGTTAACTATTTGTTTACTTAGCTAAAAACATCTGGACTTTTCTCCATAGCCAGGCTCCTGCTT GCTGAGCCGGCTTGTTCTCACCTCGAGGGGCATGGGCCTCCACCCTGCCGTTTGGGGTGAGGGGATCCTTGGCCAGGGTGGTTTCCCCGCCGTCATAGGA GGGAAGTCAGTGGGAGGCTCAGAAGGCCTTGGCTATACAACCAGTGCCATGCGGCGCAGTCAGGGTCCCATGGACGACTTCCATCAGCTCACCCGCCTCC ATCCCCAAGCAGAGTGTAGGCAAGGGCTGTGACTGCCTCAGGTAATTGGGTGATTTTGCTCCAGCAAGAGGAGAGGAAGCATGTGAGTGCCACACTGAAC CTTTCAGAAACAGCCGTGGGGTCGGGGGTGCTTGGGCCAGGCCAGGATACTTGGTGTTAAGCCTGCTCTCTCTTCAGTGCCCCGACTCGGCTTGCAAGCA GGACCTGCTGGCCTACCTGCAACGCATCGCCCTCTACTGCCACCAGCTGAACATCTGCAGCAAGGTCAAGGCCGAGGTGCAGAATCTCGGCGGGGAGCTT GTTGTCTCTGGGGTGGACAGCGCCATGTCCCTGATCCAGGCAGCCAAGAACTTGATGAATGCTGTGGTGCAGACAGTGAAGGCATCCTACGTCGCCTCTA CCAAATACCAAAAGTCACAGGGTATGGCTTCCCTCAACCTTCCTGCTGTGTCATGGAAGATGAAGGCACCAGAGAAAAAGCCATTGGTGAAGAGAGAGAA ACAGGATGAGACACAGACCAAGATTAAACGGGCATCTCAGAAGAAGCACGTGAACCCGGTGCAGGCCCTCAGCGAGTTCAAAGCTATGGACAGCATCTAA GTCTGCCCAGGCCGGCCGCCCCCACCCCTCGGGGCTCCTGAATATCAGTCACTGTTCGTCACTCAAATGAATTTGCTAAATACAACACTGATACTAGATT CCACAGGGAAATGGGCAGACTGAACCAGTCCAGGTGGTGAATTTTCCAAGAACATAGTTTAAGTTGATTAAAAATGCTTTTAGAATGCAGGAGCCTACTT CTAGCTGTATTTTTTGTATGCTTAAATAAAAATAAAAATTCATAACCAAAGAGAATCCCACATTAGCTTGTTAGTAATGCTCTGACCAAGCCGAGATGCC CATTCTCTTAGTGATGGCGGCGTTAGGGTTTGAGAGAAGGGAATTTGGCTCAACTTCAGTTGAGAGGGTGCAGTCCAGACAGCTTGACTGCTTTTAAATG ACCAAAGATGACCTGTGGTAAGCAACCTGGGCATCTTAGGAAGCAGTCCCTGGAGAAGGCATGTTCCCAGAAAGGTCTCTGGAGGGACAAACTCACTCAG TAAAACATAATGTATCATGAAGAAAACTGATTCTCTATGACATGAAATGAAAATTTTAATGCATTGTTATAATTACTAATGTACGCTGCTGCAGGACATT AATAAAGTTGCTTTTTTAGGCTACAGTGTCTCGATGCCATAATCAGAACACACTTTTTTTCCTCTTTCTCCCAGCTTCAAATGCAAATTCATCATTGGGC TCACTTCTAATAACTGCAGTGTTTCCCGCCTTGGGCTTGCAGCAGAAAAACCTGACAACATAGTGTTTGCTAAGGCAGTAATTTAGACTTTACCTTATTT GTGATTACTGTAGTGATTGATTGATTGATTACTATTAACTACAAGGTATAATTTACTATCACCTTATTTAAATTTTATGAATTAATTTGAATGTTTTTTA CACTAACTAACTTTTCCCAATAAAGTCCACTATGAAACCACGACATCCAAGAGCCCAAAGTCGTCTTCTCTGCCTTCAAGTCATAGATTTGCCCGCAGTA TCTGTGGTGCTCTGGGCCCTCCCGGTGTCCGTCTCTTCCAGGATGGGGATGCCCGGGAGGGAAACTGTCTGTGGCTCTAGGCTGCACGGCTCGTGCCAAC CCATCAGGGAGGGCCATGCCCGTTGTCCTATTGAGTGCCCCACCCTGCACCCCCACCTTGGGAATTCACATGTCCATTCCTTGAGGTTCATGTCAACCTC GGAGGCATCCCTGTCTTCATTATAGCTGACCCCTCTCCTGCGTCCTTCTGTCAGCATATCCCTTCTGCATCCTTCCCGTCACACATACATACCAAGCTAT GATGATTGATTGATAGTGGCCTTCGAGATGAAAACCATCCTTAACCCCATGATCCTTCCCAGCTGGCATCCCCACCCTAAGCAAGGTTCCCTAAAGAGAA GCTTGTTGACATTTTCTCCCCTTCCTCACTTACAGTCAGCTGTCACCTTGCTCCCTCCACCTCCCCTCGTCAAGGTGACTGCCTTCCACTGCCAGCCACA CTGGCAATTGTCTTGAGACCTCACTGTGCAAGCGGCCTGGGGGCGTGGCGGGGAGGAGGAGGAGGACAAGGACTCCTGAACGCTGCTCACTCGGCTTCCA GGACACCCCGCTCAGGTTCTGCCTGCCTTACTGGGGGCTCCTTCTCCTTTCCTGGGGCTGAGAGGTTGTGTCTGTGCCCACTCTCCTGAGCTCAGCCAGA ATCTCGCATGTAAAGACATCCAAATGCCGACTTCTTCTCCCCAGAGCCTGGCTTTTATAACTGACTGCCGCCTCACGCTCCATTCGGATGTCTAATGAAT CTCTCAGACTTGACCTGTCCAAACTGCTTGCCACCTCTACTCGCCACCGCCCACCCCCACACCCTCCATACCGTTTTCCCCACGTCACTGCCCCTCCCCC CCTTCCTCTTGCCAAATGCCTAAAAATAAAAATTCTGAGGTGGATCTGTAGCACAATGGGCTTCTAGTGACAAATCAAAATTCTGATCTTTAAAATGTAT GCACAATTTACCTGTTACCGCCTGGCCTAAGCCATCCTGCTGTTACTGCTGTGTAGCAAGTTGCAGCCAAAGCGACCTCACACGGCTCCTGAAGGTCAAG AATCTGGGAGCAGCTCAGCTGGATGGCTGTGACTCGGGGTCTCATGAAGTGGCCAAGATGCCAGCTAAGGCTGCCGTCTCTGAAGGCTTGACCTGGCGAG GATTTGCTTCCAGACTCACTCGCGTGGCTATTGACAGAAGGCCTCAGTTCCATGCCACATGGCCCGTTCCACAGAGCAGCTCACAGCTTGGTAGTAGCTG GCTTCCCCAGAGCGGTATCTGCGAGCAGCGTCCAGATGGAAGCCATGGTGTTCCCTTAAAACCTGATATGGAGGGTGATTCCCCATCATGTCTGTATTCT GTGGGTCACACAGACCAAGGCCGGTCCACTGTGGAAGGCAAGGACGAGGTTGCCAGTGCCCGGCGGTGGGTGGCCACAGCCAGCCTCCCTGCCAGTGTCC CCCACAGACAAGTTGTCCCTGTAAGACAGGTCAGCTCAGGTCATTCCGAGCTCAGACCTTCCAACGGCGTCCATGTCCCTCAGTAAAAGCCAGAGTCCTA AAGGCCTCTTGCCTGCTCTGGCCTCCTCTCACCATTCTCTGCCATCCTCCATCTGCCCCTGGTGTCCCTGAAGTCTTGAACATGCCGAGCACATCCTGCC TCGTATGCTTCCTGGCCTGGAATGCACTTGCCCCGGGACACCCTGTGCTGGCTTTAGTGTTTCCTTCCTGTCTCCTCAAGTCACCTTATTATCAAAAGGA CTCCGACAGGCTAAGTGTGGTGGCTCATGCCTGTAGTCCCAGCACTCGGGAAGGCCGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCAGGACCTGCCTG GGCAACATGGCAAAACTTTGTCTCTCTTAAAAAAAAAAAAAAAAAAAAAAAAAAG

CTNNA1.F1 (forward primer)
CTNNA1.R1 (reverse primer)
Single exon skipping

Frame 0

SFVWVFQKLYCYKAPCFVTASGSLQDPLITIKR ERRTPDLKT*YSFSVGSSSEVVIKAAFKKTHLNS WT*RKK*CTQFCNR*NDQEDQGLA*TAPQSCHG PRFRFFPGNQCSTFGID*SCKEWK*ERS*GVCP SFP*TCQQID*GCQLGLFHLK**RRCKACSNVCK PVRSPLSSGY*CCTGFSSKTTE*TGPREHGSF* RTMGKTSPCSHRCCR*HYFH**LLGCLRESHFG RCEQMCHCSPREGCGWPGPHSWCNSRPGSPGHSR SHLRDGQL*ARSLHREGSGSH*AALQHSHATFY *ASRSSRGSPQLGPCPAHG*E*VYRCFPPGI*W HPGHQESSADDKDP*GVG*L*L*DRRF*CQKQDE RPDRRRSADSWPECPGDHGSASPGAKSEDCGTG GQLPGRKEQAGC*SVQMGRQWQ*HHCAGQADVH DYDGDDRLYPR*RTTQKYIGCHQCCQENC*GRIQ DGQAWPHHCRPCK*QTCQVGLQAPPGAQAAQPG PFRVSFMGHSTFATHL*KWFLLIPA*ICKDSLE RMCMSWNLPSLGSQN*PLLLSLMSQFPLSPSGQG PRAARSHVASAVEGWCA*MGPGFFILSTVFLSH PVFIPLFPHTDRYKNTVPTAWKLYNTFHLVF*A *SREALSQLQFFSVVNKPPHHFP*NSQTLSLI*A TGCVRHKPPYTSQRL*RHP*LPHNR**GLLLPL AKCILTQTSLVLSADLHSHLDYCGRSRFPVLSS LHFLLHPAVNTVI*KCRSHGSALLP*LLRIQLIS SMVCGVSVHTTLCGVLCSSLTGRKCLLAINT*E VLCVSPFG*SFLGWS*SGSL*NSEMWSETGTGQ GPPFSL*PSPTAFFLALLYSLVFHLSLQ*VAFVI RH**RKVPQSRRVNYLFT*LKTSGLFSIARLLL AEPACSHLEGHGPPPCRLG*GDPWPGWFPRRHR REVSGRLRRPWLYNQCHAAQSGSHGRLPSAHPPP SPSRV*ARAVTASGNWVILLQQEERKHVSATLN LSETAVGSGVLGPGQDTWC*ACSLFSAPTRLAS RTCWPTCNASPSTATS*TSAARSRPRCRISAGSL LSLGWTAPCP*SRQPRT**MLWCRQ*RHPTSPL PNTKSHRVWLPSTFLLCHGR*RHQRKSHW*RER NRMRHRPRLNGHLRRST*TRCRPSASSKLWTASK SAQAGRPHPSGLLNISHCSSLK*IC*IQH*Y*I PQGNGQTEPVQVVNFPRT*FKLIKNAFRMQEPT SSCIFCMLK*K*KFITKENPTLAC**CSDQAEMP ILLVMAALGFERREFGSTSVERVQSRQLDCF*M TKDDLW*ATWAS*EAVPGEGMFPERSLEGQTHS VKHNVS*RKLILYDMK*KF*CIVIITNVRCCRTL IKLLF*ATVSRCHNQNTLFFLFLPASNANSSLG SLLITAVFPALGLQQKNLTT*CLLRQ*FRLYLI CDYCSD*LIDYY*LQGIIYYHLI*IL*INLNVFY TN*LFPIKSTMKPRHPRAQSRLLCLQVIDLPAV SVVLWALPVSVSSRMGMPGRETVCGSRLHGSCQ PIREGHARCPIECPTLHPHLGNSHVHSLRFMSTS EASLSSL*LTPLLRPSVSISLLHPSRHTYIPSY DD*LIVAFEMKTILNPMILPSWHPHPKQGSLKR SLLTFSPLPHLQSAVTLLPPPPLVKVTAFHCQPH WQLS*DLTVQAAWGRGGEEEEDKDS*TLLTRLP GHPAQVLPALLGAPSPFLGLRGCVCAHSPELSQ NLACKDIQMPTSSPQSLAFITDCRLTLHSDV**I SQT*PVQTACHLYSPPPTPTPSIPFSPRHCPSP PSSCQMPKNKNSEVDL*HNGLLVTNQNSDL*NV CTIYLLPPGLSHPAVTAV*QVAAKATSHGS*RSR IWEQLSWMAVTRGLMKWPRCQLRLPSLKA*PGE DLLPDSLAWLLTEGLSSMPHGPFHRAAHSLVVA GFPRAVSASSVQMEAMVFP*NLIWRVIPHHVCIL WVTQTKAGPLWKARTRLPVPGGGWPQPASLPVS PTDKLSL*DRSAQVIPSSDLPTASMSLSKSQSP KGLLPALASSHHSLPSSICPWCP*SLEHAEHILP RMLPGLECTCPGTPCAGFSVSFLSPQVTLLSKG LRQAKCGGSCL*SQHSGRPRWEDRLSPGVQDLP GQHGKTLSLLKKKKKKKK

Frame 1

LLFGFSRSFTVIKHHAL*QHRGRCRTP*LQ*SE KEELLT*KHDIPSV*EAAVRL**RLHSRRPI*T AGRKERSDALNSAIDKMTKKTRDLRRQLRKAVMD HVSDSFLETNVPLLVLIEAAKNGNEKEVKEYAQ VFREHANKLIEVANLACSISNNEEGVKLVRMSA SQLEALCPQVINAALALAAKPQSKLAQENMDLFK EQWEKQVRVLTDAVDDITSIDDFLAVSENHILE DVNKCVIALQEKDVDGLDRTAGAIRGRAARVIH VVTSEMDNYEPGVYTEKVLEATKLLSNTVMPRFT EQVEAAVEALSSDPAQPMDENEFIDASRLVYDG IRDIRKAVLMIRTPEELDDSDFETEDFDVRSRT SVQTEDDQLIAGQSARAIMAQLPQEQKAKIAEQV ASFQEEKSKLDAEVSKWDDSGNDIIVLAKQMCM IMMEMTDFTRGKGPLKNTSDVISAAKKIAEAGS RMDKLGRTIADHVSDRLARWVSKLLLGLRQPSLV HSG*VSWATALLPLISKNGSY*SQHRFVRIL*S GCVCPGIFHRLVAKINLCYCLLCLSSLSPPLGR GREQPEAMWPVLWKAGVPRWAQVSSSSALCFSAT PSSSPYSLTLIGTKIRFLPPGNCTIHFIWCFKP DQEKLFLSYSFSL*LTSLPTIFPEIAKLSASSR PQDV*DTSLLTQAKDCKGILDSHTIGNRAYCFHW PNVS*HKPVLSFLQISIATWTTVAAPDFLFFPL STSFCIQLSILSSENADHMALPCFHDYLEYSSS PVWSAGSQFIPLSVVYFAPASLEENVCLL*TLER CSVFLPLGKAF*AGPNLGASKTLRCGARQELGR GHPFLFDHPQLLFFLLYFTHLSSTSACSEWLLS SGINNAKCLRAEELTICLLS*KHLDFSP*PGSCL LSRLVLTSRGMGLHPAVWGEGILGQGGFPAVIG GKSVGGSEGLGYTTSAMRRSQGPMDDFHQLTRL HPQAECRQGL*LPQVIG*FCSSKRRGSM*VPH*T FQKQPWGRGCLGQARILGVKPALSSVPRLGLQA GPAGLPATHRPLLPPAEHLQQGQGRGAESRRGA CCLWGGQRHVPDPGSQELDECCGADSEGILRRLY QIPKVTGYGFPQPSCCVMEDEGTREKAIGEERE TG*DTDQD*TGISEEAREPGAGPQRVQSYGQHL SLPRPAAPTPRGS*ISVTVRHSNEFAKYNTDTRF HREMGRLNQSRW*IFQEHSLS*LKMLLECRSLL LAVFFVCLNKNKNS*PKRIPH*LVSNALTKPRC PFS**WRR*GLREGNLAQLQLRGCSPDSLTAFK* PKMTCGKQPGHLRKQSLEKACSQKGLWRDKLTQ *NIMYHEEN*FSMT*NENFNALL*LLMYAAAGH **SCFFRLQCLDAIIRTHFFSSFSQLQMQIHHWA HF**LQCFPPWACSRKT*QHSVC*GSNLDFTLF VITVVID*LITINYKV*FTITLFKFYELI*MFF TLTNFSQ*SPL*NHDIQEPKVVFSAFKS*ICPQY LWCSGPSRCPSLPGWGCPGGKLSVALGCTARAN PSGRAMPVVLLSAPPCTPTLGIHMSIP*GSCQP RRHPCLHYS*PLSCVLLSAYPFCILPVTHTYQAM MID**WPSR*KPSLTP*SFPAGIPTLSKVP*RE AC*HFLPFLTYSQLSPCSLHLPSSR*LPSTASH TGNCLETSLCKRPGGVAGRRRRTRTPERCSLGFQ DTPLRFCLPYWGLLLLSWG*EVVSVPTLLSSAR ISHVKTSKCRLLLPRAWLL*LTAASRSIRMSNE SLRLDLSKLLATSTRHRPPPHPPYRFPHVTAPPP LPLAKCLKIKILRWICSTMGF**QIKILIFKMY AQFTCYRLA*AILLLLLCSKLQPKRPHTAPEGQ ESGSSSAGWL*LGVS*SGQDAS*GCRL*RLDLAR ICFQTHSRGY*QKASVPCHMARSTEQLTAW**L ASPERYLRAASRWKPWCSLKT*YGG*FPIMSVF CGSHRPRPVHCGRQGRGCQCPAVGGHSQPPCQCP PQTSCPCKTGQLRSFRAQTFQRRPCPSVKARVL KASCLLWPPLTILCHPPSAPGVPEVLNMPSTSC LVCFLAWNALAPGHPVLALVFPSCLLKSPYYQKD SDRLSVVAHACSPSTREGRGGRIA*AQEFRTCL GNMAKLCLS*KKKKKKKK

Frame 2

FCLGFPEALLL*STMLCNSIGVVAGPPDYNKAR KKNS*LKNMIFLQCRKQQ*GCNKGCIQEDPSEQ LDVKKEVMHSILQ*IK*PRRPGTCVDSSAKLSW TTFQILSWKPMFHFWY*LKLQRMEMRKKLRSMPK FSVNMPTN*LRLPTWPVPSQIMKKV*SLFECLQ AS*KPSVLRLLMLHWL*QQNHRVNWPKRTWIFL KNNGKNKSVFSQMLSMTLLPLMTSWLSQRITFWK M*TNVSLLSKRRMWMAWTAQLVQFEAGQPGSFT *SPQRWTTMSQESTQRRFWKPLSCSPTQSCHVL LSK*KQPWKPSARTLPSPWMRMSLSMLPAWYMMA SGTSGKQC***GPLRSWMTLTLRQKILMSEAGR ASRQKTIS**LARVPGRSWLSFPRSKKRRLRNR WPASRKKRASWMLKCPNGTTVAMTSLCWPSRCA* L*WR*QTLPEVKDHSKIHRMSSVLPRKLLRQDP GWTSLAAPLQTM*VTDLPGGSPSSSWGSGSPAW SIQGKFHGPQHFCHSSLKMVLINPSIDL*GFSRA DVYVLESSIAW*PKLTSAIVSYVSVPSLPLWAG AESSQKPCGQCCGRLVCLDGPRFLHPQHCVSQP PRLHPPIPSH**VQKYGSYRLEIVQYISFGVLSL IKRSSFSVTVFLCS*QASPPFSLK*PNSQPHLG HRMCETQASLHKPKIVKASLTPTQSVIGPIASI GQMYLDTNQSCPFCRSP*PLGLLWPLPISCSFLS PLPSASSCQYCHLKMQITWLCPASMTT*NTAHL QYGLRGLSSYHSLWCTLLQPHWKKMFACYKHLR GALCFSLWVKLSRLVLIWEPLKL*DVERDRNWAG ATLFSLTIPNCFFSCFTLLTCLPPQPAVSGFCH QALITQSASEQKS*LFVYLAKNIWTFLHSQAPA C*AGLFSPRGAWASTLPFGVRGSLARVVSPPS*E GSQWEAQKALAIQPVPCGAVRVPWTTSISSPAS IPKQSVGKGCDCLR*LGDFAPARGEEACECHTE PFRNSRGVGGAWARPGYLVLSLLSLQCPDSACKQ DLLAYLQRIALYCHQLNICSKVKAEVQNLGGEL VVSGVDSAMSLIQAAKNLMNAVVQTVKASYVAS TKYQKSQGMASLNLPAVSWKMKAPEKKPLVKREK QDETQTKIKRASQKKHVNPVQALSEFKAMDSI* VCPGRPPPPLGAPEYQSLFVTQMNLLNTTLILD STGKWAD*TSPGGEFSKNIV*VD*KCF*NAGAYF *LYFLYA*IKIKIHNQRESHISLLVML*PSRDA HSLSDGGVRV*EKGIWLNFS*EGAVQTA*LLLN DQR*PVVSNLGILGSSPWRRHVPRKVSGGTNSLS KT*CIMKKTDSL*HEMKILMHCYNY*CTLLQDI NKVAFLGYSVSMP*SEHTFFPLSPSFKCKFIIG LTSNNCSVSRLGLAAEKPDNIVFAKAVI*TLPYL *LL**LID*LLLTTRYNLLSPYLNFMN*FECFL H*LTFPNKVHYETTTSKSPKSSSLPSSHRFARS ICGALGPPGVRLFQDGDAREGNCLWL*AARLVPT HQGGPCPLSY*VPHPAPPPWEFTCPFLEVHVNL GGIPVFIIADPSPASFCQHIPSASFPSHIHTKL **LIDSGLRDENHP*PHDPSQLASPP*ARFPKEK LVDIFSPSSLTVSCHLAPSTSPRQGDCLPLPAT LAIVLRPHCASGLGAWRGGGGGQGLLNAAHSAS RTPRSGSACLTGGSFSFPGAERLCLCPLS*AQPE SRM*RHPNADFFSPEPGFYN*LPPHAPFGCLMN LSDLTCPNCLPPLLATAHPHTLHTVFPTSLPLP PFLLPNA*K*KF*GGSVAQWASSDKSKF*SLKCM HNLPVTAWPKPSCCYCCVASCSQSDLTRLLKVK NLGAAQLDGCDSGSHEVAKMPAKAAVSEGLTWR GFASRLTRVAIDRRPQFHATWPVPQSSSQLGSSW LPQSGICEQRPDGSHGVPLKPDMEGDSPSCLYS VGHTDQGRSTVEGKDEVASARRWVATASLPASV PHRQVVPVRQVSSGHSELRPSNGVHVPQ*KPES* RPLACSGLLSPFSAILHLPLVSLKS*TCRAHPA SYASWPGMHLPRDTLCWL*CFLPVSSSHLIIKR TPTG*VWWLMPVVPALGKAEVGGSLEPRSSGPAW ATWQNFVSLKKKKKKKKK

Sequence CTNNA1.cAug05

TCTTTTGTTTGGGTTTTCCAGAAGCTTTACTGTTATAAAGCACCATGCTTTGTAACAGCATCGGGGTCGTTGCAGGACCCCCTGATTACAATAAAGCGAG AAAGAAGAACTCCTGACTTAAAAACATGATATTCCTTCAGTGTAGGAAGCAGCAGTGAGGTTGTAATAAAGGCTGCATTCAAGAAGACCCATCTGAACAG TGAGTTGGGTTAGCAGAGTGATGATTTTTAATGCTTGTGAGAGCCAGTGTTAGACTGCCGATTCTGCATAGAGAGATCATCTAAATGGGCAGAAGCCTGC ACAATTTATGAAGTGCTGATTTAACACCAGCCTATGCAAGGCTGGACGTAAAGAAAGAAGTGATGCACTCAATTCTGCAATAGATAAAATGACCAAGAAG ACCAGGGACTTGCGTAGACAGCTCCGCAAAGCTGTCATGGACCACGTTTCAGATTCTTTCCTGGAAACCAATGTTCCACTTTTGGTATTGATTGAAGCTG CAAAGAATGGAAATGAGAAAGAAGTTAAGGAGTATGCCCAAGTTTTCCGTGAACATGCCAACAAATTGATTGAGGTTGCCAACTTGGCCTGTTCCATCTC AAATAATGAAGAAGGTGTAAAGCTTGTTCGAATGTCTGCAAGCCAGTTAGAAGCCCTCTGTCCTCAGGTTATTAATGCTGCACTGGCTTTAGCAGCAAAA CCACAGAGTAAACTGGCCCAAGAGAACATGGATCTTTTTAAAGAACAATGGGAAAAACAAGTCCGTGTTCTCACAGATGCTGTCGATGACATTACTTCCA TTGATGACTTCTTGGCTGTCTCAGAGAATCACATTTTGGAAGATGTGAACAAATGTGTCATTGCTCTCCAAGAGAAGGATGTGGATGGCCTGGACCGCAC AGCTGGTGCAATTCGAGGCCGGGCAGCCCGGGTCATTCACGTAGTCACCTCAGAGATGGACAACTATGAGCCAGGAGTCTACACAGAGAAGGTTCTGGAA GCCACTAAGCTGCTCTCCAACACAGTCATGCCACGTTTTACTGAGCAAGTAGAAGCAGCCGTGGAAGCCCTCAGCTCGGACCCTGCCCAGCCCATGGATG AGAATGAGTTTATCGATGCTTCCCGCCTGGTATATGATGGCATCCGGGACATCAGGAAAGCAGTGCTGATGATAAGGACCCCTGAGGAGTTGGATGACTC TGACTTTGAGACAGAAGATTTTGATGTCAGAAGCAGGACGAGCGTCCAGACAGAAGACGATCAGCTGATAGCTGGCCAGAGTGCCCGGGCGATCATGGCT CAGCTTCCCCAGGAGCAAAAAGCGAAGATTGCGGAACAGGTGGCCAGCTTCCAGGAAGAAAAGAGCAAGCTGGATGCTGAAGTGTCCAAATGGGACGACA GTGGCAATGACATCATTGTGCTGGCCAAGCAGATGTGCATGATTATGATGGAGATGACAGACTTTACCCGAGGTAAAGGACCACTCAAAAATACATCGGA TGTCATCAGTGCTGCCAAGAAAATTGCTGAGGCAGGATCCAGGATGGACAAGCTTGGCCGCACCATTGCAGACCATTGCCCCGACTCGGCTTGCAAGCAG GACCTGCTGGCCTACCTGCAACGCATCGCCCTCTACTGCCACCAGCTGAACATCTGCAGCAAGGTCAAGGCCGAGGTGCAGAATCTCGGCGGGGAGCTTG TTGTCTCTGGGGTGGACAGCGCCATGTCCCTGATCCAGGCAGCCAAGAACTTGATGAATGCTGTGGTGCAGACAGTGAAGGCATCCTACGTCGCCTCTAC CAAATACCAAAAGTCACAGGGTATGGCTTCCCTCAACCTTCCTGCTGTGTCATGGAAGATGAAGGCACCAGAGAAAAAGCCATTGGTGAAGAGAGAGAAA CAGGATGAGACACAGACCAAGATTAAACGGGCATCTCAGAAGAAGCACGTGAACCCGGTGCAGGCCCTCAGCGAGTTCAAAGCTATGGACAGCATCTAAG TCTGCCCAGGCCGGCCGCCCCCACCCCTCGGGGCTCCTGAATATCAGTCACTGTTCGTCACTCAAATGAATTTGCTAAATACAACACTGATACTAGATTC CACAGGGAAATGGGCAGACTGAACCAGTCCAGGTGGTGAATTTTCCAAGAACATAGTTTAAGTTGATTAAAAATGCTTTTAGAATGCAGGAGCCTACTTC TAGCTGTATTTTTTGTATGCTTAAATAAAAATAAAAATTCATAACCAAAGAGAATCCCACATTAGCTTGTTAGTAATGCTCTGACCAAGCCGAGATGCCC ATTCTCTTAGTGATGGCGGCGTTAGGGTTTGAGAGAAGGGAATTTGGCTCAACTTCAGTTGAGAGGGTGCAGTCCAGACAGCTTGACTGCTTTTAAATGA CCAAAGATGACCTGTGGTAAGCAACCTGGGCATCTTAGGAAGCAGTCCCTGGAGAAGGCATGTTCCCAGAAAGGTCTCTGGAGGGACAAACTCACTCAGT AAAACATAATGTATCATGAAGAAAACTGATTCTCTATGACATGAAATGAAAATTTTAATGCATTGTTATAATTACTAATGTACGCTGCTGCAGGACATTA ATAAAGTTGCTTTTTTAGGCTACAGTGTCTCGATGCCATAATCAGAACACACTTTTTTTCCTCTTTCTCCCAGCTTCAAATGCAAATTCATCATTGGGCT CACTTCTAATAACTGCAGTGTTTCCCGCCTTGGGCTTGCAGCAGAAAAACCTGACAACATAGTGTTTGCTAAGGCAGTAATTTAGACTTTACCTTATTTG TGATTACTGTAGTGATTGATTGATTGATTACTATTAACTACAAGGTATAATTTACTATCACCTTATTTAAATTTTATGAATTAATTTGAATGTTTTTTAC ACTAACTAACTTTTCCCAATAAAGTCCACTATGAAACCACGACATCCAAGAGCCCAAAGTCGTCTTCTCTGCCTTCAAGTCATAGATTTGCCCGCAGTAT CTGTGGTGCTCTGGGCCCTCCCGGTGTCCGTCTCTTCCAGGATGGGGATGCCCGGGAGGGAAACTGTCTGTGGCTCTAGGCTGCACGGCTCGTGCCAACC CATCAGGGAGGGCCATGCCCGTTGTCCTATTGAGTGCCCCACCCTGCACCCCCACCTTGGGAATTCACATGTCCATTCCTTGAGGTTCATGTCAACCTCG GAGGCATCCCTGTCTTCATTATAGCTGACCCCTCTCCTGCGTCCTTCTGTCAGCATATCCCTTCTGCATCCTTCCCGTCACACATACATACCAAGCTATG ATGATTGATTGATAGTGGCCTTCGAGATGAAAACCATCCTTAACCCCATGATCCTTCCCAGCTGGCATCCCCACCCTAAGCAAGGTTCCCTAAAGAGAAG CTTGTTGACATTTTCTCCCCTTCCTCACTTACAGTCAGCTGTCACCTTGCTCCCTCCACCTCCCCTCGTCAAGGTGACTGCCTTCCACTGCCAGCCACAC TGGCAATTGTCTTGAGACCTCACTGTGCAAGCGGCCTGGGGGCGTGGCGGGGAGGAGGAGGAGGACAAGGACTCCTGAACGCTGCTCACTCGGCTTCCAG GACACCCCGCTCAGGTTCTGCCTGCCTTACTGGGGGCTCCTTCTCCTTTCCTGGGGCTGAGAGGTTGTGTCTGTGCCCACTCTCCTGAGCTCAGCCAGAA TCTCGCATGTAAAGACATCCAAATGCCGACTTCTTCTCCCCAGAGCCTGGCTTTTATAACTGACTGCCGCCTCACGCTCCATTCGGATGTCTAATGAATC TCTCAGACTTGACCTGTCCAAACTGCTTGCCACCTCTACTCGCCACCGCCCACCCCCACACCCTCCATACCGTTTTCCCCACGTCACTGCCCCTCCCCCC CTTCCTCTTGCCAAATGCCTAAAAATAAAAATTCTGAGGTGGATCTGTAGCACAATGGGCTTCTAGTGACAAATCAAAATTCTGATCTTTAAAATGTATG CACAATTTACCTGTTACCGCCTGGCCTAAGCCATCCTGCTGTTACTGCTGTGTAGCAAGTTGCAGCCAAAGCGACCTCACACGGCTCCTGAAGGTCAAGA ATCTGGGAGCAGCTCAGCTGGATGGCTGTGACTCGGGGTCTCATGAAGTGGCCAAGATGCCAGCTAAGGCTGCCGTCTCTGAAGGCTTGACCTGGCGAGG ATTTGCTTCCAGACTCACTCGCGTGGCTATTGACAGAAGGCCTCAGTTCCATGCCACATGGCCCGTTCCACAGAGCAGCTCACAGCTTGGTAGTAGCTGG CTTCCCCAGAGCGGTATCTGCGAGCAGCGTCCAGATGGAAGCCATGGTGTTCCCTTAAAACCTGATATGGAGGGTGATTCCCCATCATGTCTGTATTCTG TGGGTCACACAGACCAAGGCCGGTCCACTGTGGAAGGCAAGGACGAGGTTGCCAGTGCCCGGCGGTGGGTGGCCACAGCCAGCCTCCCTGCCAGTGTCCC CCACAGACAAGTTGTCCCTGTAAGACAGGTCAGCTCAGGTCATTCCGAGCTCAGACCTTCCAACGGCGTCCATGTCCCTCAGTAAAAGCCAGAGTCCTAA AGGCCTCTTGCCTGCTCTGGCCTCCTCTCACCATTCTCTGCCATCCTCCATCTGCCCCTGGTGTCCCTGAAGTCTTGAACATGCCGAGCACATCCTGCCT CGTATGCTTCCTGGCCTGGAATGCACTTGCCCCGGGACACCCTGTGCTGGCTTTAGTGTTTCCTTCCTGTCTCCTCAAGTCACCTTATTATCAAAAGGAC TCCGACAGGCTAAGTGTGGTGGCTCATGCCTGTAGTCCCAGCACTCGGGAAGGCCGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCAGGACCTGCCTGG GCAACATGGCAAAACTTTGTCTCTCTTAAAAAAAAAAAAAAAAAAAAAAAAAAG

CTNNA1.F1 (forward primer)
CTNNA1.R1 (reverse primer)
Single exon skipping

Frame 0

SFVWVFQKLYCYKAPCFVTASGSLQDPLITIKR ERRTPDLKT*YSFSVGSSSEVVIKAAFKKTHLNS ELG*QSDDF*CL*EPVLDCRFCIERSSKWAEAC TIYEVLI*HQPMQGWT*RKK*CTQFCNR*NDQE DQGLA*TAPQSCHGPRFRFFPGNQCSTFGID*SC KEWK*ERS*GVCPSFP*TCQQID*GCQLGLFHL K**RRCKACSNVCKPVRSPLSSGY*CCTGFSSK TTE*TGPREHGSF*RTMGKTSPCSHRCCR*HYFH **LLGCLRESHFGRCEQMCHCSPREGCGWPGPH SWCNSRPGSPGHSRSHLRDGQL*ARSLHREGSG SH*AALQHSHATFY*ASRSSRGSPQLGPCPAHG* E*VYRCFPPGI*WHPGHQESSADDKDP*GVG*L *L*DRRF*CQKQDERPDRRRSADSWPECPGDHG SASPGAKSEDCGTGGQLPGRKEQAGC*SVQMGRQ WQ*HHCAGQADVHDYDGDDRLYPR*RTTQKYIG CHQCCQENC*GRIQDGQAWPHHCRPLPRLGLQA GPAGLPATHRPLLPPAEHLQQGQGRGAESRRGAC CLWGGQRHVPDPGSQELDECCGADSEGILRRLY QIPKVTGYGFPQPSCCVMEDEGTREKAIGEERE TG*DTDQD*TGISEEAREPGAGPQRVQSYGQHLS LPRPAAPTPRGS*ISVTVRHSNEFAKYNTDTRF HREMGRLNQSRW*IFQEHSLS*LKMLLECRSLL LAVFFVCLNKNKNS*PKRIPH*LVSNALTKPRCP FS**WRR*GLREGNLAQLQLRGCSPDSLTAFK* PKMTCGKQPGHLRKQSLEKACSQKGLWRDKLTQ *NIMYHEEN*FSMT*NENFNALL*LLMYAAAGH* *SCFFRLQCLDAIIRTHFFSSFSQLQMQIHHWA HF**LQCFPPWACSRKT*QHSVC*GSNLDFTLF VITVVID*LITINYKV*FTITLFKFYELI*MFFT LTNFSQ*SPL*NHDIQEPKVVFSAFKS*ICPQY LWCSGPSRCPSLPGWGCPGGKLSVALGCTARAN PSGRAMPVVLLSAPPCTPTLGIHMSIP*GSCQPR RHPCLHYS*PLSCVLLSAYPFCILPVTHTYQAM MID**WPSR*KPSLTP*SFPAGIPTLSKVP*RE AC*HFLPFLTYSQLSPCSLHLPSSR*LPSTASHT GNCLETSLCKRPGGVAGRRRRTRTPERCSLGFQ DTPLRFCLPYWGLLLLSWG*EVVSVPTLLSSAR ISHVKTSKCRLLLPRAWLL*LTAASRSIRMSNES LRLDLSKLLATSTRHRPPPHPPYRFPHVTAPPP LPLAKCLKIKILRWICSTMGF**QIKILIFKMY AQFTCYRLA*AILLLLLCSKLQPKRPHTAPEGQE SGSSSAGWL*LGVS*SGQDAS*GCRL*RLDLAR ICFQTHSRGY*QKASVPCHMARSTEQLTAW**L ASPERYLRAASRWKPWCSLKT*YGG*FPIMSVFC GSHRPRPVHCGRQGRGCQCPAVGGHSQPPCQCP PQTSCPCKTGQLRSFRAQTFQRRPCPSVKARVL KASCLLWPPLTILCHPPSAPGVPEVLNMPSTSCL VCFLAWNALAPGHPVLALVFPSCLLKSPYYQKD SDRLSVVAHACSPSTREGRGGRIA*AQEFRTCL GNMAKLCLS*KKKKKKKK

Frame 1

LLFGFSRSFTVIKHHAL*QHRGRCRTP*LQ*SE KEELLT*KHDIPSV*EAAVRL**RLHSRRPI*T VSWVSRVMIFNACESQC*TADSA*RDHLNGQKPA QFMKC*FNTSLCKAGRKERSDALNSAIDKMTKK TRDLRRQLRKAVMDHVSDSFLETNVPLLVLIEA AKNGNEKEVKEYAQVFREHANKLIEVANLACSIS NNEEGVKLVRMSASQLEALCPQVINAALALAAK PQSKLAQENMDLFKEQWEKQVRVLTDAVDDITS IDDFLAVSENHILEDVNKCVIALQEKDVDGLDRT AGAIRGRAARVIHVVTSEMDNYEPGVYTEKVLE ATKLLSNTVMPRFTEQVEAAVEALSSDPAQPMD ENEFIDASRLVYDGIRDIRKAVLMIRTPEELDDS DFETEDFDVRSRTSVQTEDDQLIAGQSARAIMA QLPQEQKAKIAEQVASFQEEKSKLDAEVSKWDD SGNDIIVLAKQMCMIMMEMTDFTRGKGPLKNTSD VISAAKKIAEAGSRMDKLGRTIADHCPDSACKQ DLLAYLQRIALYCHQLNICSKVKAEVQNLGGEL VVSGVDSAMSLIQAAKNLMNAVVQTVKASYVAST KYQKSQGMASLNLPAVSWKMKAPEKKPLVKREK QDETQTKIKRASQKKHVNPVQALSEFKAMDSI* VCPGRPPPPLGAPEYQSLFVTQMNLLNTTLILDS TGKWAD*TSPGGEFSKNIV*VD*KCF*NAGAYF *LYFLYA*IKIKIHNQRESHISLLVML*PSRDA HSLSDGGVRV*EKGIWLNFS*EGAVQTA*LLLND QR*PVVSNLGILGSSPWRRHVPRKVSGGTNSLS KT*CIMKKTDSL*HEMKILMHCYNY*CTLLQDI NKVAFLGYSVSMP*SEHTFFPLSPSFKCKFIIGL TSNNCSVSRLGLAAEKPDNIVFAKAVI*TLPYL *LL**LID*LLLTTRYNLLSPYLNFMN*FECFL H*LTFPNKVHYETTTSKSPKSSSLPSSHRFARSI CGALGPPGVRLFQDGDAREGNCLWL*AARLVPT HQGGPCPLSY*VPHPAPPPWEFTCPFLEVHVNL GGIPVFIIADPSPASFCQHIPSASFPSHIHTKL* *LIDSGLRDENHP*PHDPSQLASPP*ARFPKEK LVDIFSPSSLTVSCHLAPSTSPRQGDCLPLPAT LAIVLRPHCASGLGAWRGGGGGQGLLNAAHSASR TPRSGSACLTGGSFSFPGAERLCLCPLS*AQPE SRM*RHPNADFFSPEPGFYN*LPPHAPFGCLMN LSDLTCPNCLPPLLATAHPHTLHTVFPTSLPLPP FLLPNA*K*KF*GGSVAQWASSDKSKF*SLKCM HNLPVTAWPKPSCCYCCVASCSQSDLTRLLKVK NLGAAQLDGCDSGSHEVAKMPAKAAVSEGLTWRG FASRLTRVAIDRRPQFHATWPVPQSSSQLGSSW LPQSGICEQRPDGSHGVPLKPDMEGDSPSCLYS VGHTDQGRSTVEGKDEVASARRWVATASLPASVP HRQVVPVRQVSSGHSELRPSNGVHVPQ*KPES* RPLACSGLLSPFSAILHLPLVSLKS*TCRAHPA SYASWPGMHLPRDTLCWL*CFLPVSSSHLIIKRT PTG*VWWLMPVVPALGKAEVGGSLEPRSSGPAW ATWQNFVSLKKKKKKKKK

Frame 2

FCLGFPEALLL*STMLCNSIGVVAGPPDYNKAR KKNS*LKNMIFLQCRKQQ*GCNKGCIQEDPSEQ *VGLAE**FLMLVRASVRLPILHREII*MGRSL HNL*SADLTPAYARLDVKKEVMHSILQ*IK*PRR PGTCVDSSAKLSWTTFQILSWKPMFHFWY*LKL QRMEMRKKLRSMPKFSVNMPTN*LRLPTWPVPS QIMKKV*SLFECLQAS*KPSVLRLLMLHWL*QQN HRVNWPKRTWIFLKNNGKNKSVFSQMLSMTLLP LMTSWLSQRITFWKM*TNVSLLSKRRMWMAWTA QLVQFEAGQPGSFT*SPQRWTTMSQESTQRRFWK PLSCSPTQSCHVLLSK*KQPWKPSARTLPSPWM RMSLSMLPAWYMMASGTSGKQC***GPLRSWMT LTLRQKILMSEAGRASRQKTIS**LARVPGRSWL SFPRSKKRRLRNRWPASRKKRASWMLKCPNGTT VAMTSLCWPSRCA*L*WR*QTLPEVKDHSKIHR MSSVLPRKLLRQDPGWTSLAAPLQTIAPTRLASR TCWPTCNASPSTATS*TSAARSRPRCRISAGSL LSLGWTAPCP*SRQPRT**MLWCRQ*RHPTSPL PNTKSHRVWLPSTFLLCHGR*RHQRKSHW*RERN RMRHRPRLNGHLRRST*TRCRPSASSKLWTASK SAQAGRPHPSGLLNISHCSSLK*IC*IQH*Y*I PQGNGQTEPVQVVNFPRT*FKLIKNAFRMQEPTS SCIFCMLK*K*KFITKENPTLAC**CSDQAEMP ILLVMAALGFERREFGSTSVERVQSRQLDCF*M TKDDLW*ATWAS*EAVPGEGMFPERSLEGQTHSV KHNVS*RKLILYDMK*KF*CIVIITNVRCCRTL IKLLF*ATVSRCHNQNTLFFLFLPASNANSSLG SLLITAVFPALGLQQKNLTT*CLLRQ*FRLYLIC DYCSD*LIDYY*LQGIIYYHLI*IL*INLNVFY TN*LFPIKSTMKPRHPRAQSRLLCLQVIDLPAV SVVLWALPVSVSSRMGMPGRETVCGSRLHGSCQP IREGHARCPIECPTLHPHLGNSHVHSLRFMSTS EASLSSL*LTPLLRPSVSISLLHPSRHTYIPSY DD*LIVAFEMKTILNPMILPSWHPHPKQGSLKRS LLTFSPLPHLQSAVTLLPPPPLVKVTAFHCQPH WQLS*DLTVQAAWGRGGEEEEDKDS*TLLTRLP GHPAQVLPALLGAPSPFLGLRGCVCAHSPELSQN LACKDIQMPTSSPQSLAFITDCRLTLHSDV**I SQT*PVQTACHLYSPPPTPTPSIPFSPRHCPSP PSSCQMPKNKNSEVDL*HNGLLVTNQNSDL*NVC TIYLLPPGLSHPAVTAV*QVAAKATSHGS*RSR IWEQLSWMAVTRGLMKWPRCQLRLPSLKA*PGE DLLPDSLAWLLTEGLSSMPHGPFHRAAHSLVVAG FPRAVSASSVQMEAMVFP*NLIWRVIPHHVCIL WVTQTKAGPLWKARTRLPVPGGGWPQPASLPVS PTDKLSL*DRSAQVIPSSDLPTASMSLSKSQSPK GLLPALASSHHSLPSSICPWCP*SLEHAEHILP RMLPGLECTCPGTPCAGFSVSFLSPQVTLLSKG LRQAKCGGSCL*SQHSGRPRWEDRLSPGVQDLPG QHGKTLSLLKKKKKKKK

Sequence CTNNA1.aAug05

CCATTTCCTCCTCCTAGCCGGACTGGAGGGAGACAAAGCAGCGCCCGTCTGCTTCGGGCCTCTGGAATTTAGCGCTCGCCCAGCTAGCCGCAGAAATGAC TGCTGTCCATGCAGGCAACATAAACTTCAAGTGGGATCCTAAAAGTCTAGAGATCAGGACTCTGGCAGTTGAGAGACTGTTGGAGCCTCTTGTTACACAG GTTACAACCCTTGTAAACACCAATAGTAAAGGGCCCTCTAATAAGAAGAGAGGTCGTTCTAAGAAGGCCCATGTTTTGGCTGCATCTGTTGAACAAGCAA CTGAGAATTTCTTGGAGAAGGGGGATAAAATTGCGAAGGAGAGCCAGTTTCTCAAGGAGGAGCTTGTGGCTGCTGTAGAAGATGTTCGAAAACAAGGTGA TTTGATGAAGGCTGCTGCAGGAGAGTTCGCAGATGATCCCTGCTCTTCTGTGAAGCGAGGCAACATGGTTCGGGCAGCTCGAGCTTTGCTCTCTGCTGTT ACCCGGTTGCTGATTTTGGCTGACATGGCAGATGTCTACAAATTACTTGTTCAGCTGAAAGTTGTGGAAGATGGTATCTTGAAGTTGAGGAATGCTGGCA ATGAACAAGACTTAGGAATCCAGTATAAAGCCCTAAAACCTGAAGTGGATAAGCTGAACATTATGGCAGCCAAAAGACAACAGGAATTGAAAGATGTTGG CCATCGTGATCAGATGGCTGCAGCTAGAGGAATCCTGCAGAAGAACGTTCCGATCCTCTATACTGCATCCCAGGCATGCCTACAGCACCCTGATGTCGCA GCCTATAAGGCCAACAGGGACCTGATATACAAGCAGCTGCAGCAGGCGGTCACAGGCATTTCCAATGCAGCCCAGGCCACTGCCTCAGACGATGCCTCAC AGCACCAGGGTGGAGGAGGAGGAGAACTGGCATATGCACTCAATAACTTTGACAAACAAATCATTGTGGACCCCTTGAGCTTCAGCGAGGAGCGCTTTAG GCCTTCCCTGGAGGAGCGTCTGGAAAGCATCATTAGTGGGGCTGCCTTGATGGCCGACTCGTCCTGCACGCGTGATGACCGTCGTGAGCGAATTGTGGCA GAGTGTAATGCTGTCCGCCAGGCCCTGCAGGACCTGCTTTCGGAGTACATGGGCAATGCTGGACGTAAAGAAAGAAGTGATGCACTCAATTCTGCAATAG ATAAAATGACCAAGAAGACCAGGGACTTGCGTAGACAGCTCCGCAAAGCTGTCATGGACCACGTTTCAGATTCTTTCCTGGAAACCAATGTTCCACTTTT GGTATTGATTGAAGCTGCAAAGAATGGAAATGAGAAAGAAGTTAAGGAGTATGCCCAAGTTTTCCGTGAACATGCCAACAAATTGATTGAGGTTGCCAAC TTGGCCTGTTCCATCTCAAATAATGAAGAAGGTGTAAAGCTTGTTCGAATGTCTGCAAGCCAGTTAGAAGCCCTCTGTCCTCAGGTTATTAATGCTGCAC TGGCTTTAGCAGCAAAACCACAGAGTAAACTGGCCCAAGAGAACATGGATCTTTTTAAAGAACAATGGGAAAAACAAGTCCGTGTTCTCACAGATGCTGT CGATGACATTACTTCCATTGATGACTTCTTGGCTGTCTCAGAGAATCACATTTTGGAAGATGTGAACAAATGTGTCATTGCTCTCCAAGAGAAGGATGTG GATGGCCTGGACCGCACAGCTGGTGCAATTCGAGGCCGGGCAGCCCGGGTCATTCACGTAGTCACCTCAGAGATGGACAACTATGAGCCAGGAGTCTACA CAGAGAAGGTTCTGGAAGCCACTAAGCTGCTCTCCAACACAGTCATGCCACGTTTTACTGAGCAAGTAGAAGCAGCCGTGGAAGCCCTCAGCTCGGACCC TGCCCAGCCCATGGATGAGAATGAGTTTATCGATGCTTCCCGCCTGGTATATGATGGCATCCGGGACATCAGGAAAGCAGTGCTGATGATAAGGACCCCT GAGGAGTTGGATGACTCTGACTTTGAGACAGAAGATTTTGATGTCAGAAGCAGGACGAGCGTCCAGACAGAAGACGATCAGCTGATAGCTGGCCAGAGTG CCCGGGCGATCATGGCTCAGCTTCCCCAGGAGCAAAAAGCGAAGATTGCGGAACAGGTGGCCAGCTTCCAGGAAGAAAAGAGCAAGCTGGATGCTGAAGT GTCCAAATGGGACGACAGTGGCAATGACATCATTGTGCTGGCCAAGCAGATGTGCATGATTATGATGGAGATGACAGACTTTACCCGAGGTAAAGGACCA CTCAAAAATACATCGGATGTCATCAGTGCTGCCAAGAAAATTGCTGAGGCAGGATCCAGGATGGACAAGCTTGGCCGCACCATTGCAGACCATTGCCCCG ACTCGGCTTGCAAGCAGGACCTGCTGGCCTACCTGCAACGCATCGCCCTCTACTGCCACCAGCTGAACATCTGCAGCAAGGTCAAGGCCGAGGTGCAGAA TCTCGGCGGGGAGCTTGTTGTCTCTGGGGTGGACAGCGCCATGTCCCTGATCCAGGCAGCCAAGAACTTGATGAATGCTGTGGTGCAGACAGTGAAGGCA TCCTACGTCGCCTCTACCAAATACCAAAAGTCACAGGGTATGGCTTCCCTCAACCTTCCTGCTGTGTCATGGAAGATGAAGGCACCAGAGAAAAAGCCAT TGGTGAAGAGAGAGAAACAGGATGAGACACAGACCAAGATTAAACGGGCATCTCAGAAGAAGCACGTGAACCCGGTGCAGGCCCTCAGCGAGTTCAAAGC TATGGACAGCATCTAAGTCTGCCCAGGCCGGCCGCCCCCACCCCTCGGGGCTCCTGAATATCAGTCACTGTTCGTCACTCAAATGAATTTGCTAAATACA ACACTGATACTAGATTCCACAGGGAAATGGGCAGACTGAACCAGTCCAGGTGGTGAATTTTCCAAGAACATAGTTTAAGTTGATTAAAAATGCTTTTAGA ATGCAGGAGCCTACTTCTAGCTGTATTTTTTGTATGCTTAAATAAAAATAAAAATTCATAACCAAAGAGAATCCCACATTAGCTTGTTAGTAATGCTCTG ACCAAGCCGAGATGCCCATTCTCTTAGTGATGGCGGCGTTAGGGTTTGAGAGAAGGGAATTTGGCTCAACTTCAGTTGAGAGGGTGCAGTCCAGACAGCT TGACTGCTTTTAAATGACCAAAGATGACCTGTGGTAAGCAACCTGGGCATCTTAGGAAGCAGTCCCTGGAGAAGGCATGTTCCCAGAAAGGTCTCTGGAG GGACAAACTCACTCAGTAAAACATAATGTATCATGAAGAAAACTGATTCTCTATGACATGAAATGAAAATTTTAATGCATTGTTATAATTACTAATGTAC GCTGCTGCAGGACATTAATAAAGTTGCTTTTTTAGGCTACAGTGTCTCGATGCCATAATCAGAACACACTTTTTTTCCTCTTTCTCCCAGCTTCAAATGC AAATTCATCATTGGGCTCACTTCTAATAACTGCAGTGTTTCCCGCCTTGGGCTTGCAGCAGAAAAACCTGACAACATAGTGTTTGCTAAGGCAGTAATTT AGACTTTACCTTATTTGTGATTACTGTAGTGATTGATTGATTGATTACTATTAACTACAAGGTATAATTTACTATCACCTTATTTAAATTTTATGAATTA ATTTGAATGTTTTTTACACTAACTAACTTTTCCCAATAAAGTCCACTATGAAACCACGACATCCAAGAGCCCAAAGTCGTCTTCTCTGCCTTCAAGTCAT AGATTTGCCCGCAGTATCTGTGGTGCTCTGGGCCCTCCCGGTGTCCGTCTCTTCCAGGATGGGGATGCCCGGGAGGGAAACTGTCTGTGGCTCTAGGCTG CACGGCTCGTGCCAACCCATCAGGGAGGGCCATGCCCGTTGTCCTATTGAGTGCCCCACCCTGCACCCCCACCTTGGGAATTCACATGTCCATTCCTTGA GGTTCATGTCAACCTCGGAGGCATCCCTGTCTTCATTATAGCTGACCCCTCTCCTGCGTCCTTCTGTCAGCATATCCCTTCTGCATCCTTCCCGTCACAC ATACATACCAAGCTATGATGATTGATTGATAGTGGCCTTCGAGATGAAAACCATCCTTAACCCCATGATCCTTCCCAGCTGGCATCCCCACCCTAAGCAA GGTTCCCTAAAGAGAAGCTTGTTGACATTTTCTCCCCTTCCTCACTTACAGTCAGCTGTCACCTTGCTCCCTCCACCTCCCCTCGTCAAGGTGACTGCCT TCCACTGCCAGCCACACTGGCAATTGTCTTGAGACCTCACTGTGCAAGCGGCCTGGGGGCGTGGCGGGGAGGAGGAGGAGGACAAGGACTCCTGAACGCT GCTCACTCGGCTTCCAGGACACCCCGCTCAGGTTCTGCCTGCCTTACTGGGGGCTCCTTCTCCTTTCCTGGGGCTGAGAGGTTGTGTCTGTGCCCACTCT CCTGAGCTCAGCCAGAATCTCGCATGTAAAGACATCCAAATGCCGACTTCTTCTCCCCAGAGCCTGGCTTTTATAACTGACTGCCGCCTCACGCTCCATT CGGATGTCTAATGAATCTCTCAGACTTGACCTGTCCAAACTGCTTGCCACCTCTACTCGCCACCGCCCACCCCCACACCCTCCATACCGTTTTCCCCACG TCACTGCCCCTCCCCCCCTTCCTCTTGCCAAATGCCTAAAAATAAAAATTCTGAGGTGGATCTGTAGCACAATGGGCTTCTAGTGACAAATCAAAATTCT GATCTTTAAAATGTATGCACAATTTACCTGTTACCGCCTGGCCTAAGCCATCCTGCTGTTACTGCTGTGTAGCAAGTTGCAGCCAAAGCGACCTCACACG GCTCCTGAAGGTCAAGAATCTGGGAGCAGCTCAGCTGGATGGCTGTGACTCGGGGTCTCATGAAGTGGCCAAGATGCCAGCTAAGGCTGCCGTCTCTGAA GGCTTGACCTGGCGAGGATTTGCTTCCAGACTCACTCGCGTGGCTATTGACAGAAGGCCTCAGTTCCATGCCACATGGCCCGTTCCACAGAGCAGCTCAC AGCTTGGTAGTAGCTGGCTTCCCCAGAGCGGTATCTGCGAGCAGCGTCCAGATGGAAGCCATGGTGTTCCCTTAAAACCTGATATGGAGGGTGATTCCCC ATCATGTCTGTATTCTGTGGGTCACACAGACCAAGGCCGGTCCACTGTGGAAGGCAAGGACGAGGTTGCCAGTGCCCGGCGGTGGGTGGCCACAGCCAGC CTCCCTGCCAGTGTCCCCCACAGACAAGTTGTCCCTGTAAGACAGGTCAGCTCAGGTCATTCCGAGCTCAGACCTTCCAACGGCGTCCATGTCCCTCAGT AAAAGCCAGAGTCCTAAAGGCCTCTTGCCTGCTCTGGCCTCCTCTCACCATTCTCTGCCATCCTCCATCTGCCCCTGGTGTCCCTGAAGTCTTGAACATG CCGAGCACATCCTGCCTCGTATGCTTCCTGGCCTGGAATGCACTTGCCCCGGGACACCCTGTGCTGGCTTTAGTGTTTCCTTCCTGTCTCCTCAAGTCAC CTTATTATCAAAAGGACTCCGACAGGCTAAGTGTGGTGGCTCATGCCTGTAGTCCCAGCACTCGGGAAGGCCGAGGTGGGAGGATCGCTTGAGCCCAGGA GTTCAGGACCTGCCTGGGCAACATGGCAAAACTTTGTCTCTCTTAAAAAAAAAAAAAAAAAAAAAAAAAAG

CTNNA1.F1 (forward primer)
CTNNA1.R1 (reverse primer)
Single exon skipping

Frame 0

PFPPPSRTGGRQSSARLLRASGI*RSPS*PQK* LLSMQAT*TSSGILKV*RSGLWQLRDCWSLLLHR LQPL*TPIVKGPLIRREVVLRRPMFWLHLLNKQ LRISWRRGIKLRRRASFSRRSLWLL*KMFENKV I**RLLQESSQMIPALL*SEATWFGQLELCSLLL PGC*FWLTWQMSTNYLFS*KLWKMVS*S*GMLA MNKT*ESSIKP*NLKWIS*TLWQPKDNRN*KML AIVIRWLQLEESCRRTFRSSILHPRHAYSTLMSQ PIRPTGT*YTSSCSRRSQAFPMQPRPLPQTMPH STRVEEEENWHMHSITLTNKSLWTP*ASARSAL GLPWRSVWKASLVGLP*WPTRPARVMTVVSELWQ SVMLSARPCRTCFRSTWAMLDVKKEVMHSILQ* IK*PRRPGTCVDSSAKLSWTTFQILSWKPMFHF WY*LKLQRMEMRKKLRSMPKFSVNMPTN*LRLPT WPVPSQIMKKV*SLFECLQAS*KPSVLRLLMLH WL*QQNHRVNWPKRTWIFLKNNGKNKSVFSQML SMTLLPLMTSWLSQRITFWKM*TNVSLLSKRRMW MAWTAQLVQFEAGQPGSFT*SPQRWTTMSQEST QRRFWKPLSCSPTQSCHVLLSK*KQPWKPSART LPSPWMRMSLSMLPAWYMMASGTSGKQC***GPL RSWMTLTLRQKILMSEAGRASRQKTIS**LARV PGRSWLSFPRSKKRRLRNRWPASRKKRASWMLK CPNGTTVAMTSLCWPSRCA*L*WR*QTLPEVKDH SKIHRMSSVLPRKLLRQDPGWTSLAAPLQTIAP TRLASRTCWPTCNASPSTATS*TSAARSRPRCR ISAGSLLSLGWTAPCP*SRQPRT**MLWCRQ*RH PTSPLPNTKSHRVWLPSTFLLCHGR*RHQRKSH W*RERNRMRHRPRLNGHLRRST*TRCRPSASSK LWTASKSAQAGRPHPSGLLNISHCSSLK*IC*IQ H*Y*IPQGNGQTEPVQVVNFPRT*FKLIKNAFR MQEPTSSCIFCMLK*K*KFITKENPTLAC**CS DQAEMPILLVMAALGFERREFGSTSVERVQSRQL DCF*MTKDDLW*ATWAS*EAVPGEGMFPERSLE GQTHSVKHNVS*RKLILYDMK*KF*CIVIITNV RCCRTLIKLLF*ATVSRCHNQNTLFFLFLPASNA NSSLGSLLITAVFPALGLQQKNLTT*CLLRQ*F RLYLICDYCSD*LIDYY*LQGIIYYHLI*IL*I NLNVFYTN*LFPIKSTMKPRHPRAQSRLLCLQVI DLPAVSVVLWALPVSVSSRMGMPGRETVCGSRL HGSCQPIREGHARCPIECPTLHPHLGNSHVHSL RFMSTSEASLSSL*LTPLLRPSVSISLLHPSRHT YIPSYDD*LIVAFEMKTILNPMILPSWHPHPKQ GSLKRSLLTFSPLPHLQSAVTLLPPPPLVKVTA FHCQPHWQLS*DLTVQAAWGRGGEEEEDKDS*TL LTRLPGHPAQVLPALLGAPSPFLGLRGCVCAHS PELSQNLACKDIQMPTSSPQSLAFITDCRLTLH SDV**ISQT*PVQTACHLYSPPPTPTPSIPFSPR HCPSPPSSCQMPKNKNSEVDL*HNGLLVTNQNS DL*NVCTIYLLPPGLSHPAVTAV*QVAAKATSH GS*RSRIWEQLSWMAVTRGLMKWPRCQLRLPSLK A*PGEDLLPDSLAWLLTEGLSSMPHGPFHRAAH SLVVAGFPRAVSASSVQMEAMVFP*NLIWRVIP HHVCILWVTQTKAGPLWKARTRLPVPGGGWPQPA SLPVSPTDKLSL*DRSAQVIPSSDLPTASMSLS KSQSPKGLLPALASSHHSLPSSICPWCP*SLEH AEHILPRMLPGLECTCPGTPCAGFSVSFLSPQVT LLSKGLRQAKCGGSCL*SQHSGRPRWEDRLSPG VQDLPGQHGKTLSLLKKKKKKKK

Frame 1

HFLLLAGLEGDKAAPVCFGPLEFSARPASRRND CCPCRQHKLQVGS*KSRDQDSGS*ETVGASCYT GYNPCKHQ**RAL**EERSF*EGPCFGCIC*TSN *EFLGEGG*NCEGEPVSQGGACGCCRRCSKTR* FDEGCCRRVRR*SLLFCEARQHGSGSSSFALCC YPVADFG*HGRCLQITCSAESCGRWYLEVEECWQ *TRLRNPV*SPKT*SG*AEHYGSQKTTGIERCW PS*SDGCS*RNPAEERSDPLYCIPGMPTAP*CR SL*GQQGPDIQAAAAGGHRHFQCSPGHCLRRCLT APGWRRRRTGICTQ*L*QTNHCGPLELQRGAL* AFPGGASGKHH*WGCLDGRLVLHA**PS*ANCG RV*CCPPGPAGPAFGVHGQCWT*RKK*CTQFCNR *NDQEDQGLA*TAPQSCHGPRFRFFPGNQCSTF GID*SCKEWK*ERS*GVCPSFP*TCQQID*GCQ LGLFHLK**RRCKACSNVCKPVRSPLSSGY*CCT GFSSKTTE*TGPREHGSF*RTMGKTSPCSHRCC R*HYFH**LLGCLRESHFGRCEQMCHCSPREGC GWPGPHSWCNSRPGSPGHSRSHLRDGQL*ARSLH REGSGSH*AALQHSHATFY*ASRSSRGSPQLGP CPAHG*E*VYRCFPPGI*WHPGHQESSADDKDP *GVG*L*L*DRRF*CQKQDERPDRRRSADSWPEC PGDHGSASPGAKSEDCGTGGQLPGRKEQAGC*S VQMGRQWQ*HHCAGQADVHDYDGDDRLYPR*RT TQKYIGCHQCCQENC*GRIQDGQAWPHHCRPLPR LGLQAGPAGLPATHRPLLPPAEHLQQGQGRGAE SRRGACCLWGGQRHVPDPGSQELDECCGADSEG ILRRLYQIPKVTGYGFPQPSCCVMEDEGTREKAI GEERETG*DTDQD*TGISEEAREPGAGPQRVQS YGQHLSLPRPAAPTPRGS*ISVTVRHSNEFAKY NTDTRFHREMGRLNQSRW*IFQEHSLS*LKMLLE CRSLLLAVFFVCLNKNKNS*PKRIPH*LVSNAL TKPRCPFS**WRR*GLREGNLAQLQLRGCSPDS LTAFK*PKMTCGKQPGHLRKQSLEKACSQKGLWR DKLTQ*NIMYHEEN*FSMT*NENFNALL*LLMY AAAGH**SCFFRLQCLDAIIRTHFFSSFSQLQM QIHHWAHF**LQCFPPWACSRKT*QHSVC*GSNL DFTLFVITVVID*LITINYKV*FTITLFKFYEL I*MFFTLTNFSQ*SPL*NHDIQEPKVVFSAFKS *ICPQYLWCSGPSRCPSLPGWGCPGGKLSVALGC TARANPSGRAMPVVLLSAPPCTPTLGIHMSIP* GSCQPRRHPCLHYS*PLSCVLLSAYPFCILPVT HTYQAMMID**WPSR*KPSLTP*SFPAGIPTLSK VP*REAC*HFLPFLTYSQLSPCSLHLPSSR*LP STASHTGNCLETSLCKRPGGVAGRRRRTRTPER CSLGFQDTPLRFCLPYWGLLLLSWG*EVVSVPTL LSSARISHVKTSKCRLLLPRAWLL*LTAASRSI RMSNESLRLDLSKLLATSTRHRPPPHPPYRFPH VTAPPPLPLAKCLKIKILRWICSTMGF**QIKIL IFKMYAQFTCYRLA*AILLLLLCSKLQPKRPHT APEGQESGSSSAGWL*LGVS*SGQDAS*GCRL* RLDLARICFQTHSRGY*QKASVPCHMARSTEQLT AW**LASPERYLRAASRWKPWCSLKT*YGG*FP IMSVFCGSHRPRPVHCGRQGRGCQCPAVGGHSQ PPCQCPPQTSCPCKTGQLRSFRAQTFQRRPCPSV KARVLKASCLLWPPLTILCHPPSAPGVPEVLNM PSTSCLVCFLAWNALAPGHPVLALVFPSCLLKS PYYQKDSDRLSVVAHACSPSTREGRGGRIA*AQE FRTCLGNMAKLCLS*KKKKKKKK

Frame 2

ISSS*PDWRETKQRPSASGLWNLALAQLAAEMT AVHAGNINFKWDPKSLEIRTLAVERLLEPLVTQ VTTLVNTNSKGPSNKKRGRSKKAHVLAASVEQA TENFLEKGDKIAKESQFLKEELVAAVEDVRKQGD LMKAAAGEFADDPCSSVKRGNMVRAARALLSAV TRLLILADMADVYKLLVQLKVVEDGILKLRNAG NEQDLGIQYKALKPEVDKLNIMAAKRQQELKDVG HRDQMAAARGILQKNVPILYTASQACLQHPDVA AYKANRDLIYKQLQQAVTGISNAAQATASDDAS QHQGGGGGELAYALNNFDKQIIVDPLSFSEERFR PSLEERLESIISGAALMADSSCTRDDRRERIVA ECNAVRQALQDLLSEYMGNAGRKERSDALNSAI DKMTKKTRDLRRQLRKAVMDHVSDSFLETNVPLL VLIEAAKNGNEKEVKEYAQVFREHANKLIEVAN LACSISNNEEGVKLVRMSASQLEALCPQVINAA LALAAKPQSKLAQENMDLFKEQWEKQVRVLTDAV DDITSIDDFLAVSENHILEDVNKCVIALQEKDV DGLDRTAGAIRGRAARVIHVVTSEMDNYEPGVY TEKVLEATKLLSNTVMPRFTEQVEAAVEALSSDP AQPMDENEFIDASRLVYDGIRDIRKAVLMIRTP EELDDSDFETEDFDVRSRTSVQTEDDQLIAGQS ARAIMAQLPQEQKAKIAEQVASFQEEKSKLDAEV SKWDDSGNDIIVLAKQMCMIMMEMTDFTRGKGP LKNTSDVISAAKKIAEAGSRMDKLGRTIADHCP DSACKQDLLAYLQRIALYCHQLNICSKVKAEVQN LGGELVVSGVDSAMSLIQAAKNLMNAVVQTVKA SYVASTKYQKSQGMASLNLPAVSWKMKAPEKKP LVKREKQDETQTKIKRASQKKHVNPVQALSEFKA MDSI*VCPGRPPPPLGAPEYQSLFVTQMNLLNT TLILDSTGKWAD*TSPGGEFSKNIV*VD*KCF* NAGAYF*LYFLYA*IKIKIHNQRESHISLLVML* PSRDAHSLSDGGVRV*EKGIWLNFS*EGAVQTA *LLLNDQR*PVVSNLGILGSSPWRRHVPRKVSG GTNSLSKT*CIMKKTDSL*HEMKILMHCYNY*CT LLQDINKVAFLGYSVSMP*SEHTFFPLSPSFKC KFIIGLTSNNCSVSRLGLAAEKPDNIVFAKAVI *TLPYL*LL**LID*LLLTTRYNLLSPYLNFMN* FECFLH*LTFPNKVHYETTTSKSPKSSSLPSSH RFARSICGALGPPGVRLFQDGDAREGNCLWL*A ARLVPTHQGGPCPLSY*VPHPAPPPWEFTCPFLE VHVNLGGIPVFIIADPSPASFCQHIPSASFPSH IHTKL**LIDSGLRDENHP*PHDPSQLASPP*A RFPKEKLVDIFSPSSLTVSCHLAPSTSPRQGDCL PLPATLAIVLRPHCASGLGAWRGGGGGQGLLNA AHSASRTPRSGSACLTGGSFSFPGAERLCLCPL S*AQPESRM*RHPNADFFSPEPGFYN*LPPHAPF GCLMNLSDLTCPNCLPPLLATAHPHTLHTVFPT SLPLPPFLLPNA*K*KF*GGSVAQWASSDKSKF *SLKCMHNLPVTAWPKPSCCYCCVASCSQSDLTR LLKVKNLGAAQLDGCDSGSHEVAKMPAKAAVSE GLTWRGFASRLTRVAIDRRPQFHATWPVPQSSS QLGSSWLPQSGICEQRPDGSHGVPLKPDMEGDSP SCLYSVGHTDQGRSTVEGKDEVASARRWVATAS LPASVPHRQVVPVRQVSSGHSELRPSNGVHVPQ *KPES*RPLACSGLLSPFSAILHLPLVSLKS*TC RAHPASYASWPGMHLPRDTLCWL*CFLPVSSSH LIIKRTPTG*VWWLMPVVPALGKAEVGGSLEPR SSGPAWATWQNFVSLKKKKKKKKK

Sequence CTNNA1.bAug05

GGATACCCCAAACCAAGACAGATGTCTCACAAAACACAAGCAGAGATGTCAGTCTTCAGGCCTCGCATGGAGAAGATGAAAGAGAGGGGTGGCTTTTCCA AAGCCCAGCGTCAGCACGAAGCTTCCACGAGATGATTTTCCCTCTTATTTTGAAGTCCTGAAATGACTGCTGTCCATGCAGGCAACATAAACTTCAAGTG GGATCCTAAAAGTCTAGAGATCAGGACTCTGGCAGTTGAGAGACTGTTGGAGCCTCTTGTTACACAGGTTACAACCCTTGTAAACACCAATAGTAAAGGG CCCTCTAATAAGAAGAGAGGTCGTTCTAAGAAGGCCCATGTTTTGGCTGCATCTGTTGAACAAGCAACTGAGAATTTCTTGGAGAAGGGGGATAAAATTG CGAAGGAGAGCCAGTTTCTCAAGGAGGAGCTTGTGGCTGCTGTAGAAGATGTTCGAAAACAAGGTGATTTGATGAAGGCTGCTGCAGGAGAGTTCGCAGA TGATCCCTGCTCTTCTGTGAAGCGAGGCAACATGGTTCGGGCAGCTCGAGCTTTGCTCTCTGCTGTTACCCGGTTGCTGATTTTGGCTGACATGGCAGAT GTCTACAAATTACTTGTTCAGCTGAAAGTTGTGGAAGATGGTATCTTGAAGTTGAGGAATGCTGGCAATGAACAAGACTTAGGAATCCAGTATAAAGCCC TAAAACCTGAAGTGGATAAGCTGAACATTATGGCAGCCAAAAGACAACAGGAATTGAAAGATGTTGGCCATCGTGATCAGATGGCTGCAGCTAGAGGAAT CCTGCAGAAGAACGTTCCGATCCTCTATACTGCATCCCAGGCATGCCTACAGCACCCTGATGTCGCAGCCTATAAGGCCAACAGGGACCTGATATACAAG CAGCTGCAGCAGGCGGTCACAGGCATTTCCAATGCAGCCCAGGCCACTGCCTCAGACGATGCCTCACAGCACCAGGGTGGAGGAGGAGGAGAACTGGCAT ATGCACTCAATAACTTTGACAAACAAATCATTGTGGACCCCTTGAGCTTCAGCGAGGAGCGCTTTAGGCCTTCCCTGGAGGAGCGTCTGGAAAGCATCAT TAGTGGGGCTGCCTTGATGGCCGACTCGTCCTGCACGCGTGATGACCGTCGTGAGCGAATTGTGGCAGAGTGTAATGCTGTCCGCCAGGCCCTGCAGGAC CTGCTTTCGGAGTACATGGGCAATGCTGGACGTAAAGAAAGAAGTGATGCACTCAATTCTGCAATAGATAAAATGACCAAGAAGACCAGGGACTTGCGTA GACAGCTCCGCAAAGCTGTCATGGACCACGTTTCAGATTCTTTCCTGGAAACCAATGTTCCACTTTTGGTATTGATTGAAGCTGCAAAGAATGGAAATGA GAAAGAAGTTAAGGAGTATGCCCAAGTTTTCCGTGAACATGCCAACAAATTGATTGAGGTTGCCAACTTGGCCTGTTCCATCTCAAATAATGAAGAAGGT GTAAAGCTTGTTCGAATGTCTGCAAGCCAGTTAGAAGCCCTCTGTCCTCAGGTTATTAATGCTGCACTGGCTTTAGCAGCAAAACCACAGAGTAAACTGG CCCAAGAGAACATGGATCTTTTTAAAGAACAATGGGAAAAACAAGTCCGTGTTCTCACAGATGCTGTCGATGACATTACTTCCATTGATGACTTCTTGGC TGTCTCAGAGAATCACATTTTGGAAGATGTGAACAAATGTGTCATTGCTCTCCAAGAGAAGGATGTGGATGGCCTGGACCGCACAGCTGGTGCAATTCGA GGCCGGGCAGCCCGGGTCATTCACGTAGTCACCTCAGAGATGGACAACTATGAGCCAGGAGTCTACACAGAGAAGGTTCTGGAAGCCACTAAGCTGCTCT CCAACACAGTCATGCCACGTTTTACTGAGCAAGTAGAAGCAGCCGTGGAAGCCCTCAGCTCGGACCCTGCCCAGCCCATGGATGAGAATGAGTTTATCGA TGCTTCCCGCCTGGTATATGATGGCATCCGGGACATCAGGAAAGCAGTGCTGATGATAAGGACCCCTGAGGAGTTGGATGACTCTGACTTTGAGACAGAA GATTTTGATGTCAGAAGCAGGACGAGCGTCCAGACAGAAGACGATCAGCTGATAGCTGGCCAGAGTGCCCGGGCGATCATGGCTCAGCTTCCCCAGGAGC AAAAAGCGAAGATTGCGGAACAGGTGGCCAGCTTCCAGGAAGAAAAGAGCAAGCTGGATGCTGAAGTGTCCAAATGGGACGACAGTGGCAATGACATCAT TGTGCTGGCCAAGCAGATGTGCATGATTATGATGGAGATGACAGACTTTACCCGAGGTAAAGGACCACTCAAAAATACATCGGATGTCATCAGTGCTGCC AAGAAAATTGCTGAGGCAGGATCCAGGATGGACAAGCTTGGCCGCACCATTGCAGACCATTGCCCCGACTCGGCTTGCAAGCAGGACCTGCTGGCCTACC TGCAACGCATCGCCCTCTACTGCCACCAGCTGAACATCTGCAGCAAGGTCAAGGCCGAGGTGCAGAATCTCGGCGGGGAGCTTGTTGTCTCTGGGAACTG TGACACCTGCGGGGCACTGCAAGGGCTGAAAGGCTGGCCTCCTCCCCTTTGCTGGCCACTCACTGGGTGGACAGCGCCATGTCCCTGATCCAGGCAGCCA AGAACTTGATGAATGCTGTGGTGCAGACAGTGAAGGCATCCTACGTCGCCTCTACCAAATACCAAAAGTCACAGGGTATGGCTTCCCTCAACCTTCCTGC TGTGTCATGGAAGATGAAGGCACCAGAGAAAAAGCCATTGGTGAAGAGAGAGAAACAGGATGAGACACAGACCAAGATTAAACGGGCATCTCAGAAGAAG CACGTGAACCCGGTGCAGGCCCTCAGCGAGTTCAAAGCTATGGACAGCATCTAAGTCTGCCCAGGCCGGCCGCCCCCACCCCTCGGGGCTCCTGAATATC AGTCACTGTTCGTCACTCAAATGAATTTGCTAAATACAACACTGATACTAGATTCCACAGGGAAATGGGCAGACTGAACCAGTCCAGGTGGTGAATTTTC CAAGAACATAGTTTAAGTTGATTAAAAATGCTTTTAGAATGCAGGAGCCTACTTCTAGCTGTATTTTTTGTATGCTTAAATAAAAATAAAAATTCATAAC CAAAGAGAATCCCACATTAGCTTGTTAGTAATGCTCTGACCAAGCCGAGATGCCCATTCTCTTAGTGATGGCGGCGTTAGGGTTTGAGAGAAGGGAATTT GGCTCAACTTCAGTTGAGAGGGTGCAGTCCAGACAGCTTGACTGCTTTTAAATGACCAAAGATGACCTGTGGTAAGCAACCTGGGCATCTTAGGAAGCAG TCCCTGGAGAAGGCATGTTCCCAGAAAGGTCTCTGGAGGGACAAACTCACTCAGTAAAACATAATGTATCATGAAGAAAACTGATTCTCTATGACATGAA ATGAAAATTTTAATGCATTGTTATAATTACTAATGTACGCTGCTGCAGGACATTAATAAAGTTGCTTTTTTAGGCTACAGTGTCTCGATGCCATAATCAG AACACACTTTTTTTCCTCTTTCTCCCAGCTTCAAATGCAAATTCATCATTGGGCTCACTTCTAATAACTGCAGTGTTTCCCGCCTTGGGCTTGCAGCAGA AAAACCTGACAACATAGTGTTTGCTAAGGCAGTAATTTAGACTTTACCTTATTTGTGATTACTGTAGTGATTGATTGATTGATTACTATTAACTACAAGG TATAATTTACTATCACCTTATTTAAATTTTATGAATTAATTTGAATGTTTTTTACACTAACTAACTTTTCCCAATAAAGTCCACTATGAAACCACGACAT CCAAGAGCCCAAAGTCGTCTTCTCTGCCTTCAAGTCATAGATTTGCCCGCAGTATCTGTGGTGCTCTGGGCCCTCCCGGTGTCCGTCTCTTCCAGGATGG GGATGCCCGGGAGGGAAACTGTCTGTGGCTCTAGGCTGCACGGCTCGTGCCAACCCATCAGGGAGGGCCATGCCCGTTGTCCTATTGAGTGCCCCACCCT GCACCCCCACCTTGGGAATTCACATGTCCATTCCTTGAGGTTCATGTCAACCTCGGAGGCATCCCTGTCTTCATTATAGCTGACCCCTCTCCTGCGTCCT TCTGTCAGCATATCCCTTCTGCATCCTTCCCGTCACACATACATACCAAGCTATGATGATTGATTGATAGTGGCCTTCGAGATGAAAACCATCCTTAACC CCATGATCCTTCCCAGCTGGCATCCCCACCCTAAGCAAGGTTCCCTAAAGAGAAGCTTGTTGACATTTTCTCCCCTTCCTCACTTACAGTCAGCTGTCAC CTTGCTCCCTCCACCTCCCCTCGTCAAGGTGACTGCCTTCCACTGCCAGCCACACTGGCAATTGTCTTGAGACCTCACTGTGCAAGCGGCCTGGGGGCGT GGCGGGGAGGAGGAGGAGGACAAGGACTCCTGAACGCTGCTCACTCGGCTTCCAGGACACCCCGCTCAGGTTCTGCCTGCCTTACTGGGGGCTCCTTCTC CTTTCCTGGGGCTGAGAGGTTGTGTCTGTGCCCACTCTCCTGAGCTCAGCCAGAATCTCGCATGTAAAGACATCCAAATGCCGACTTCTTCTCCCCAGAG CCTGGCTTTTATAACTGACTGCCGCCTCACGCTCCATTCGGATGTCTAATGAATCTCTCAGACTTGACCTGTCCAAACTGCTTGCCACCTCTACTCGCCA CCGCCCACCCCCACACCCTCCATACCGTTTTCCCCACGTCACTGCCCCTCCCCCCCTTCCTCTTGCCAAATGCCTAAAAATAAAAATTCTGAGGTGGATC TGTAGCACAATGGGCTTCTAGTGACAAATCAAAATTCTGATCTTTAAAATGTATGCACAATTTACCTGTTACCGCCTGGCCTAAGCCATCCTGCTGTTAC TGCTGTGTAGCAAGTTGCAGCCAAAGCGACCTCACACGGCTCCTGAAGGTCAAGAATCTGGGAGCAGCTCAGCTGGATGGCTGTGACTCGGGGTCTCATG AAGTGGCCAAGATGCCAGCTAAGGCTGCCGTCTCTGAAGGCTTGACCTGGCGAGGATTTGCTTCCAGACTCACTCGCGTGGCTATTGACAGAAGGCCTCA GTTCCATGCCACATGGCCCGTTCCACAGAGCAGCTCACAGCTTGGTAGTAGCTGGCTTCCCCAGAGCGGTATCTGCGAGCAGCGTCCAGATGGAAGCCAT GGTGTTCCCTTAAAACCTGATATGGAGGGTGATTCCCCATCATGTCTGTATTCTGTGGGTCACACAGACCAAGGCCGGTCCACTGTGGAAGGCAAGGACG AGGTTGCCAGTGCCCGGCGGTGGGTGGCCACAGCCAGCCTCCCTGCCAGTGTCCCCCACAGACAAGTTGTCCCTGTAAGACAGGTCAGCTCAGGTCATTC CGAGCTCAGACCTTCCAACGGCGTCCATGTCCCTCAGTAAAAGCCAGAGTCCTAAAGGCCTCTTGCCTGCTCTGGCCTCCTCTCACCATTCTCTGCCATC CTCCATCTGCCCCTGGTGTCCCTGAAGTCTTGAACATGCCGAGCACATCCTGCCTCGTATGCTTCCTGGCCTGGAATGCACTTGCCCCGGGACACCCTGT GCTGGCTTTAGTGTTTCCTTCCTGTCTCCTCAAGTCACCTTATTATCAAAAGGACTCCGACAGGCTAAGTGTGGTGGCTCATGCCTGTAGTCCCAGCACT CGGGAAGGCCGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCAGGACCTGCCTGGGCAACATGGCAAAACTTTGTCTCTCTTAAAAAAAAAAAAAAAAAA AAAAAAAAG

CTNNA1.F1 (forward primer)
CTNNA1.R1 (reverse primer)
Single exon skipping

Frame 0

GYPKPRQMSHKTQAEMSVFRPRMEKMKERGGFS KAQRQHEASTR*FSLLF*SPEMTAVHAGNINFKW DPKSLEIRTLAVERLLEPLVTQVTTLVNTNSKG PSNKKRGRSKKAHVLAASVEQATENFLEKGDKI AKESQFLKEELVAAVEDVRKQGDLMKAAAGEFAD DPCSSVKRGNMVRAARALLSAVTRLLILADMAD VYKLLVQLKVVEDGILKLRNAGNEQDLGIQYKA LKPEVDKLNIMAAKRQQELKDVGHRDQMAAARGI LQKNVPILYTASQACLQHPDVAAYKANRDLIYK QLQQAVTGISNAAQATASDDASQHQGGGGGELA YALNNFDKQIIVDPLSFSEERFRPSLEERLESII SGAALMADSSCTRDDRRERIVAECNAVRQALQD LLSEYMGNAGRKERSDALNSAIDKMTKKTRDLR RQLRKAVMDHVSDSFLETNVPLLVLIEAAKNGNE KEVKEYAQVFREHANKLIEVANLACSISNNEEG VKLVRMSASQLEALCPQVINAALALAAKPQSKL AQENMDLFKEQWEKQVRVLTDAVDDITSIDDFLA VSENHILEDVNKCVIALQEKDVDGLDRTAGAIR GRAARVIHVVTSEMDNYEPGVYTEKVLEATKLL SNTVMPRFTEQVEAAVEALSSDPAQPMDENEFID ASRLVYDGIRDIRKAVLMIRTPEELDDSDFETE DFDVRSRTSVQTEDDQLIAGQSARAIMAQLPQE QKAKIAEQVASFQEEKSKLDAEVSKWDDSGNDII VLAKQMCMIMMEMTDFTRGKGPLKNTSDVISAA KKIAEAGSRMDKLGRTIADHCPDSACKQDLLAY LQRIALYCHQLNICSKVKAEVQNLGGELVVSGNC DTCGALQGLKGWPPPLCWPLTGWTAPCP*SRQP RT**MLWCRQ*RHPTSPLPNTKSHRVWLPSTFL LCHGR*RHQRKSHW*RERNRMRHRPRLNGHLRRS T*TRCRPSASSKLWTASKSAQAGRPHPSGLLNI SHCSSLK*IC*IQH*Y*IPQGNGQTEPVQVVNF PRT*FKLIKNAFRMQEPTSSCIFCMLK*K*KFIT KENPTLAC**CSDQAEMPILLVMAALGFERREF GSTSVERVQSRQLDCF*MTKDDLW*ATWAS*EA VPGEGMFPERSLEGQTHSVKHNVS*RKLILYDMK *KF*CIVIITNVRCCRTLIKLLF*ATVSRCHNQ NTLFFLFLPASNANSSLGSLLITAVFPALGLQQ KNLTT*CLLRQ*FRLYLICDYCSD*LIDYY*LQG IIYYHLI*IL*INLNVFYTN*LFPIKSTMKPRH PRAQSRLLCLQVIDLPAVSVVLWALPVSVSSRM GMPGRETVCGSRLHGSCQPIREGHARCPIECPTL HPHLGNSHVHSLRFMSTSEASLSSL*LTPLLRP SVSISLLHPSRHTYIPSYDD*LIVAFEMKTILN PMILPSWHPHPKQGSLKRSLLTFSPLPHLQSAVT LLPPPPLVKVTAFHCQPHWQLS*DLTVQAAWGR GGEEEEDKDS*TLLTRLPGHPAQVLPALLGAPS PFLGLRGCVCAHSPELSQNLACKDIQMPTSSPQS LAFITDCRLTLHSDV**ISQT*PVQTACHLYSP PPTPTPSIPFSPRHCPSPPSSCQMPKNKNSEVD L*HNGLLVTNQNSDL*NVCTIYLLPPGLSHPAVT AV*QVAAKATSHGS*RSRIWEQLSWMAVTRGLM KWPRCQLRLPSLKA*PGEDLLPDSLAWLLTEGL SSMPHGPFHRAAHSLVVAGFPRAVSASSVQMEAM VFP*NLIWRVIPHHVCILWVTQTKAGPLWKART RLPVPGGGWPQPASLPVSPTDKLSL*DRSAQVI PSSDLPTASMSLSKSQSPKGLLPALASSHHSLPS SICPWCP*SLEHAEHILPRMLPGLECTCPGTPC AGFSVSFLSPQVTLLSKGLRQAKCGGSCL*SQH SGRPRWEDRLSPGVQDLPGQHGKTLSLLKKKKKK KK

Frame 1

DTPNQDRCLTKHKQRCQSSGLAWRR*KRGVAFP KPSVSTKLPRDDFPSYFEVLK*LLSMQAT*TSS GILKV*RSGLWQLRDCWSLLLHRLQPL*TPIVKG PLIRREVVLRRPMFWLHLLNKQLRISWRRGIKL RRRASFSRRSLWLL*KMFENKVI**RLLQESSQ MIPALL*SEATWFGQLELCSLLLPGC*FWLTWQM STNYLFS*KLWKMVS*S*GMLAMNKT*ESSIKP *NLKWIS*TLWQPKDNRN*KMLAIVIRWLQLEE SCRRTFRSSILHPRHAYSTLMSQPIRPTGT*YTS SCSRRSQAFPMQPRPLPQTMPHSTRVEEEENWH MHSITLTNKSLWTP*ASARSALGLPWRSVWKAS LVGLP*WPTRPARVMTVVSELWQSVMLSARPCRT CFRSTWAMLDVKKEVMHSILQ*IK*PRRPGTCV DSSAKLSWTTFQILSWKPMFHFWY*LKLQRMEM RKKLRSMPKFSVNMPTN*LRLPTWPVPSQIMKKV *SLFECLQAS*KPSVLRLLMLHWL*QQNHRVNW PKRTWIFLKNNGKNKSVFSQMLSMTLLPLMTSW LSQRITFWKM*TNVSLLSKRRMWMAWTAQLVQFE AGQPGSFT*SPQRWTTMSQESTQRRFWKPLSCS PTQSCHVLLSK*KQPWKPSARTLPSPWMRMSLS MLPAWYMMASGTSGKQC***GPLRSWMTLTLRQK ILMSEAGRASRQKTIS**LARVPGRSWLSFPRS KKRRLRNRWPASRKKRASWMLKCPNGTTVAMTS LCWPSRCA*L*WR*QTLPEVKDHSKIHRMSSVLP RKLLRQDPGWTSLAAPLQTIAPTRLASRTCWPT CNASPSTATS*TSAARSRPRCRISAGSLLSLGT VTPAGHCKG*KAGLLPFAGHSLGGQRHVPDPGSQ ELDECCGADSEGILRRLYQIPKVTGYGFPQPSC CVMEDEGTREKAIGEERETG*DTDQD*TGISEE AREPGAGPQRVQSYGQHLSLPRPAAPTPRGS*IS VTVRHSNEFAKYNTDTRFHREMGRLNQSRW*IF QEHSLS*LKMLLECRSLLLAVFFVCLNKNKNS* PKRIPH*LVSNALTKPRCPFS**WRR*GLREGNL AQLQLRGCSPDSLTAFK*PKMTCGKQPGHLRKQ SLEKACSQKGLWRDKLTQ*NIMYHEEN*FSMT* NENFNALL*LLMYAAAGH**SCFFRLQCLDAIIR THFFSSFSQLQMQIHHWAHF**LQCFPPWACSR KT*QHSVC*GSNLDFTLFVITVVID*LITINYK V*FTITLFKFYELI*MFFTLTNFSQ*SPL*NHDI QEPKVVFSAFKS*ICPQYLWCSGPSRCPSLPGW GCPGGKLSVALGCTARANPSGRAMPVVLLSAPP CTPTLGIHMSIP*GSCQPRRHPCLHYS*PLSCVL LSAYPFCILPVTHTYQAMMID**WPSR*KPSLT P*SFPAGIPTLSKVP*REAC*HFLPFLTYSQLS PCSLHLPSSR*LPSTASHTGNCLETSLCKRPGGV AGRRRRTRTPERCSLGFQDTPLRFCLPYWGLLL LSWG*EVVSVPTLLSSARISHVKTSKCRLLLPR AWLL*LTAASRSIRMSNESLRLDLSKLLATSTRH RPPPHPPYRFPHVTAPPPLPLAKCLKIKILRWI CSTMGF**QIKILIFKMYAQFTCYRLA*AILLL LLCSKLQPKRPHTAPEGQESGSSSAGWL*LGVS* SGQDAS*GCRL*RLDLARICFQTHSRGY*QKAS VPCHMARSTEQLTAW**LASPERYLRAASRWKP WCSLKT*YGG*FPIMSVFCGSHRPRPVHCGRQGR GCQCPAVGGHSQPPCQCPPQTSCPCKTGQLRSF RAQTFQRRPCPSVKARVLKASCLLWPPLTILCH PPSAPGVPEVLNMPSTSCLVCFLAWNALAPGHPV LALVFPSCLLKSPYYQKDSDRLSVVAHACSPST REGRGGRIA*AQEFRTCLGNMAKLCLS*KKKKK KKK

Frame 2

IPQTKTDVSQNTSRDVSLQASHGEDEREGWLFQ SPASARSFHEMIFPLILKS*NDCCPCRQHKLQV GS*KSRDQDSGS*ETVGASCYTGYNPCKHQ**R AL**EERSF*EGPCFGCIC*TSN*EFLGEGG*NC EGEPVSQGGACGCCRRCSKTR*FDEGCCRRVRR *SLLFCEARQHGSGSSSFALCCYPVADFG*HGR CLQITCSAESCGRWYLEVEECWQ*TRLRNPV*SP KT*SG*AEHYGSQKTTGIERCWPS*SDGCS*RN PAEERSDPLYCIPGMPTAP*CRSL*GQQGPDIQ AAAAGGHRHFQCSPGHCLRRCLTAPGWRRRRTGI CTQ*L*QTNHCGPLELQRGAL*AFPGGASGKHH *WGCLDGRLVLHA**PS*ANCGRV*CCPPGPAG PAFGVHGQCWT*RKK*CTQFCNR*NDQEDQGLA* TAPQSCHGPRFRFFPGNQCSTFGID*SCKEWK* ERS*GVCPSFP*TCQQID*GCQLGLFHLK**RR CKACSNVCKPVRSPLSSGY*CCTGFSSKTTE*TG PREHGSF*RTMGKTSPCSHRCCR*HYFH**LLG CLRESHFGRCEQMCHCSPREGCGWPGPHSWCNS RPGSPGHSRSHLRDGQL*ARSLHREGSGSH*AAL QHSHATFY*ASRSSRGSPQLGPCPAHG*E*VYR CFPPGI*WHPGHQESSADDKDP*GVG*L*L*DR RF*CQKQDERPDRRRSADSWPECPGDHGSASPGA KSEDCGTGGQLPGRKEQAGC*SVQMGRQWQ*HH CAGQADVHDYDGDDRLYPR*RTTQKYIGCHQCC QENC*GRIQDGQAWPHHCRPLPRLGLQAGPAGLP ATHRPLLPPAEHLQQGQGRGAESRRGACCLWEL *HLRGTARAERLASSPLLATHWVDSAMSLIQAA KNLMNAVVQTVKASYVASTKYQKSQGMASLNLPA VSWKMKAPEKKPLVKREKQDETQTKIKRASQKK HVNPVQALSEFKAMDSI*VCPGRPPPPLGAPEY QSLFVTQMNLLNTTLILDSTGKWAD*TSPGGEFS KNIV*VD*KCF*NAGAYF*LYFLYA*IKIKIHN QRESHISLLVML*PSRDAHSLSDGGVRV*EKGI WLNFS*EGAVQTA*LLLNDQR*PVVSNLGILGSS PWRRHVPRKVSGGTNSLSKT*CIMKKTDSL*HE MKILMHCYNY*CTLLQDINKVAFLGYSVSMP*S EHTFFPLSPSFKCKFIIGLTSNNCSVSRLGLAAE KPDNIVFAKAVI*TLPYL*LL**LID*LLLTTR YNLLSPYLNFMN*FECFLH*LTFPNKVHYETTT SKSPKSSSLPSSHRFARSICGALGPPGVRLFQDG DAREGNCLWL*AARLVPTHQGGPCPLSY*VPHP APPPWEFTCPFLEVHVNLGGIPVFIIADPSPAS FCQHIPSASFPSHIHTKL**LIDSGLRDENHP*P HDPSQLASPP*ARFPKEKLVDIFSPSSLTVSCH LAPSTSPRQGDCLPLPATLAIVLRPHCASGLGA WRGGGGGQGLLNAAHSASRTPRSGSACLTGGSFS FPGAERLCLCPLS*AQPESRM*RHPNADFFSPE PGFYN*LPPHAPFGCLMNLSDLTCPNCLPPLLA TAHPHTLHTVFPTSLPLPPFLLPNA*K*KF*GGS VAQWASSDKSKF*SLKCMHNLPVTAWPKPSCCY CCVASCSQSDLTRLLKVKNLGAAQLDGCDSGSH EVAKMPAKAAVSEGLTWRGFASRLTRVAIDRRPQ FHATWPVPQSSSQLGSSWLPQSGICEQRPDGSH GVPLKPDMEGDSPSCLYSVGHTDQGRSTVEGKD EVASARRWVATASLPASVPHRQVVPVRQVSSGHS ELRPSNGVHVPQ*KPES*RPLACSGLLSPFSAI LHLPLVSLKS*TCRAHPASYASWPGMHLPRDTL CWL*CFLPVSSSHLIIKRTPTG*VWWLMPVVPAL GKAEVGGSLEPRSSGPAWATWQNFVSLKKKKKK KKK